CN110372807A - A kind of Polysaccharide in Pleurotus eryngii extraction process - Google Patents
A kind of Polysaccharide in Pleurotus eryngii extraction process Download PDFInfo
- Publication number
- CN110372807A CN110372807A CN201910626044.8A CN201910626044A CN110372807A CN 110372807 A CN110372807 A CN 110372807A CN 201910626044 A CN201910626044 A CN 201910626044A CN 110372807 A CN110372807 A CN 110372807A
- Authority
- CN
- China
- Prior art keywords
- pleurotus eryngii
- polysaccharide
- powder
- extraction process
- additive amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
A kind of Polysaccharide in Pleurotus eryngii extraction process after the steps include: that (1) cleans up fresh Pleurotus eryngii, cuts fritter and is put into drying in vacuum oven, to Pleurotus eryngii perseverance quality, be put into pulverizer and crush, sieving obtains Pleurotus eryngii powder, spare；(2) it is transferred in heatproof container after mixing Pleurotus eryngii powder with 10-20 times of Pleurotus eryngii powder weight of distilled water, is cooled to room temperature after boiling water boiling 10min；(3) adjusting pH value is 5-7, is added cellulase and neutral proteinase in coolant liquid, water enzyme digestion after a certain period of time, ultrasonic wave extraction；(4) the Pleurotus eryngii degradation in step (3) is filtered and takes filtrate, rotary evaporation concentration.The present invention adds cellulase and neutral proteinase and digests to Pleurotus eryngii, strict control enzymatic hydrolysis condition, and is ultrasonically treated in enzymolysis process, extracts polysaccharide, greatly improves recovery rate, then analyses polysaccharide with dehydrated alcohol alcohol, obtains Polysaccharide in Pleurotus eryngii.
Description
Technical field
The present invention relates to Pleurotus eryngii technical field, especially a kind of Polysaccharide in Pleurotus eryngii extraction process.
Background technique
Pleurotus eryngii also known as pleurotus eryngii are gained the name because of the mouthfeel of its fragrance and bacterial context plumpness such as abalone with almond, apricot
Abalone mushroom bacterial context is plump, and quality is tender and crisp, is suitble to fresh-keeping, processing, and Pleurotus eryngii is full of nutrition, rich in protein, carbohydrate, dimension life
Element and the minerals such as calcium, magnesium copper, zinc, can be improved immune function of human body, to human body have anticancer, reducing blood lipid, ease constipation stomach and
Beautification function firmly gets liking for people, but Pleurotus eryngii leftover bits and pieces is often dropped, and cheap, nutritive value is poor with Pleurotus eryngii
It is not smaller, also contain polysaccharide isoreactivity substance, polysaccharide is one of pharmaceutical component important in edible mushroom, and Polysaccharide in Pleurotus eryngii is as one
A special immunomodulator, it is mediated with strong host in activated T lymphocytes, antibody can be stimulated to be formed, enhanced
Body immunity plays antitumaous effect, so, it is very heavy to provide a kind of process that polysaccharide is extracted using Pleurotus eryngii leftover bits and pieces
It wants.
Summary of the invention
The present invention provides in order to solve the above problem, and Pleurotus eryngii leftover bits and pieces high efficiency extraction apricot can be made full use of by providing one kind
The technique of abalone mushroom polysaccharide.
The technical solution used in the present invention:
A kind of Polysaccharide in Pleurotus eryngii extraction process, the steps include:
(1) after fresh Pleurotus eryngii being cleaned up, cut fritter be put into vacuum oven it is dry, to Pleurotus eryngii perseverance quality,
It is put into pulverizer and crushes, sieving obtains Pleurotus eryngii powder, spare；
(2) it is transferred in heatproof container after mixing Pleurotus eryngii powder with 10-20 times of Pleurotus eryngii powder weight of distilled water,
It is cooled to room temperature after boiling water boiling 10min；
(3) it is 5-7 that the coolant liquid in step (2), which adjusts pH value to it, and cellulase and neutral egg are added in coolant liquid
White enzyme, water enzyme digestion after a certain period of time, supersonic frequency be 120-140kHz under, ultrasonic wave extraction 4-7min, interval 8-
12min, at ultrasonic 160-180kHz, ultrasonic wave extraction 4-7min, boiling water enzyme deactivation 10min terminate enzymatic hydrolysis, obtain Pleurotus eryngii enzyme
Solve slurries；
(4) the Pleurotus eryngii degradation in step (3) is filtered and takes filtrate, rotary evaporation is concentrated to the 1/4-1/ of former filtrate volume
After 3, dehydrated alcohol is added with the volume ratio of 1:3, at 4 DEG C of constant temperature overnight, 20min is centrifuged at 4000r/min, discards supernatant
Liquid takes precipitating, and gained precipitating is cleaned 3-4 times repeatedly with dehydrated alcohol, and vacuum freeze drying obtains Pleurotus eryngii Thick many candies.
The partial size of Pleurotus eryngii powder is 100-200 mesh in the step (1).
The additive amount of described step (3) cellulase is the 0.8- of Pleurotus eryngii powder additive amount weight in step (2)
1%.
The additive amount of neutral proteinase in the step (3) is Pleurotus eryngii powder additive amount weight in step (2)
0.1-0.3%.
The temperature of water enzyme digestion is 40-55 DEG C in the step (3).
The time of water enzyme digestion is 2-4h in the step (3).
Beneficial effects of the present invention: the present invention adds cellulase and neutral proteinase and digests to Pleurotus eryngii, strict control
Enzymatic hydrolysis condition, and be ultrasonically treated in enzymolysis process, polysaccharide is extracted, recovery rate is greatly improved, then with dehydrated alcohol alcohol
Polysaccharide is analysed, Polysaccharide in Pleurotus eryngii is obtained.
Specific embodiment
A kind of Polysaccharide in Pleurotus eryngii extraction process, the steps include:
(1) after fresh Pleurotus eryngii being cleaned up, cut fritter be put into vacuum oven it is dry, to Pleurotus eryngii perseverance quality,
It is put into pulverizer and crushes, sieving obtains Pleurotus eryngii powder, spare；
(2) it is transferred in heatproof container after mixing Pleurotus eryngii powder with 10-20 times of Pleurotus eryngii powder weight of distilled water,
It is cooled to room temperature after boiling water boiling 10min；
(3) it is 5-7 that the coolant liquid in step (2), which adjusts pH value to it, and cellulase and neutral egg are added in coolant liquid
White enzyme, water enzyme digestion after a certain period of time, supersonic frequency be 120-140kHz under, ultrasonic wave extraction 4-7min, interval 8-
12min, at ultrasonic 160-180kHz, ultrasonic wave extraction 4-7min, boiling water enzyme deactivation 10min terminate enzymatic hydrolysis, obtain Pleurotus eryngii enzyme
Solve slurries；
(4) the Pleurotus eryngii degradation in step (3) is filtered and takes filtrate, rotary evaporation is concentrated to the 1/4-1/ of former filtrate volume
After 3, dehydrated alcohol is added with the volume ratio of 1:3, at 4 DEG C of constant temperature overnight, 20min is centrifuged at 4000r/min, discards supernatant
Liquid takes precipitating, and gained precipitating is cleaned 3-4 times repeatedly with dehydrated alcohol, and vacuum freeze drying obtains Pleurotus eryngii Thick many candies.
The partial size of Pleurotus eryngii powder is 100-200 mesh in the step (1).
The additive amount of described step (3) cellulase is the 0.8- of Pleurotus eryngii powder additive amount weight in step (2)
1%.
The additive amount of neutral proteinase in the step (3) is Pleurotus eryngii powder additive amount weight in step (2)
0.1-0.3%.
The temperature of water enzyme digestion is 40-55 DEG C in the step (3).
The time of water enzyme digestion is 2-4h in the step (3).
Below in conjunction with specific embodiment, the present invention will be described in detail.
Embodiment 1
A kind of Polysaccharide in Pleurotus eryngii extraction process, the steps include:
(1) after fresh Pleurotus eryngii being cleaned up, cut fritter be put into vacuum oven it is dry, to Pleurotus eryngii perseverance quality,
It is put into pulverizer and crushes, sieving obtains Pleurotus eryngii powder, and the partial size of Pleurotus eryngii powder is 100 mesh, spare；
(2) it is transferred in heatproof container, boils after mixing Pleurotus eryngii powder with 10 times of Pleurotus eryngii powder weight of distilled water
It is cooled to room temperature after boiling 10min；
(3) it is 5 that the coolant liquid in step (2), which adjusts pH value to it, and cellulase and neutral protein are added in coolant liquid
Enzyme, the additive amount of cellulase are the 0.8% of Pleurotus eryngii powder additive amount weight, and the additive amount of neutral proteinase is pleurotus eryngii powder
The 0.1% of last additive amount weight, after digesting 2h at 40 DEG C of bath temperature, in the case where supersonic frequency is 120kHz, ultrasonic wave extraction
4min, interval 8min, at ultrasonic 160kHz, ultrasonic wave extraction 4min, boiling water enzyme deactivation 10min terminate enzymatic hydrolysis, obtain Pleurotus eryngii
Digest slurries；
(4) the Pleurotus eryngii degradation in step (3) is filtered after taking filtrate, rotary evaporation to be concentrated to the 1/4 of former filtrate volume,
Dehydrated alcohol is added with the volume ratio of 1:3, at 4 DEG C of constant temperature overnight, is centrifuged 20min at 4000r/min, discards supernatant liquid,
Precipitating is taken, gained precipitating is cleaned 3 times repeatedly with dehydrated alcohol, and vacuum freeze drying obtains Pleurotus eryngii Thick many candies.
Embodiment 2
A kind of Polysaccharide in Pleurotus eryngii extraction process, the steps include:
(1) after fresh Pleurotus eryngii being cleaned up, cut fritter be put into vacuum oven it is dry, to Pleurotus eryngii perseverance quality,
It is put into pulverizer and crushes, sieving obtains Pleurotus eryngii powder, and the partial size of Pleurotus eryngii powder is 150 mesh, spare；
(2) it is transferred in heatproof container, boils after mixing Pleurotus eryngii powder with 15 times of Pleurotus eryngii powder weight of distilled water
It is cooled to room temperature after boiling 10min；
(3) it is 5.5 that the coolant liquid in step (2), which adjusts pH value to it, and cellulase and neutral egg are added in coolant liquid
White enzyme, the additive amount of cellulase are the 0.85% of Pleurotus eryngii powder additive amount weight, and the additive amount of neutral proteinase is apricot Bao
The 0.15% of mushroom powder end additive amount weight, after digesting 2.5h at 45 DEG C of bath temperature, in the case where supersonic frequency is 130kHz, ultrasonic wave
5min, interval 9min are extracted, at ultrasonic 170kHz, ultrasonic wave extraction 5min, boiling water enzyme deactivation 10min terminate enzymatic hydrolysis, obtain apricot
Abalone mushroom digests slurries；
(4) the Pleurotus eryngii degradation in step (3) is filtered after taking filtrate, rotary evaporation to be concentrated to the 1/3 of former filtrate volume,
Dehydrated alcohol is added with the volume ratio of 1:3, at 4 DEG C of constant temperature overnight, is centrifuged 20min at 4000r/min, discards supernatant liquid,
Precipitating is taken, gained precipitating is cleaned 4 times repeatedly with dehydrated alcohol, and vacuum freeze drying obtains Pleurotus eryngii Thick many candies.
Embodiment 3
A kind of Polysaccharide in Pleurotus eryngii extraction process, the steps include:
(1) after fresh Pleurotus eryngii being cleaned up, cut fritter be put into vacuum oven it is dry, to Pleurotus eryngii perseverance quality,
It is put into pulverizer and crushes, sieving obtains Pleurotus eryngii powder, and the partial size of Pleurotus eryngii powder is 180 mesh, spare；
(2) it is transferred in heatproof container, boils after mixing Pleurotus eryngii powder with 18 times of Pleurotus eryngii powder weight of distilled water
It is cooled to room temperature after boiling 10min；
(3) it is 6 that the coolant liquid in step (2), which adjusts pH value to it, and cellulase and neutral protein are added in coolant liquid
Enzyme, the additive amount of cellulase are the 0.9% of Pleurotus eryngii powder additive amount weight, and the additive amount of neutral proteinase is pleurotus eryngii powder
The 0.2% of last additive amount weight, after digesting 3h at 50 DEG C of bath temperature, in the case where supersonic frequency is 130kHz, ultrasonic wave extraction
6min, interval 10min, at ultrasonic 170kHz, ultrasonic wave extraction 6min, boiling water enzyme deactivation 10min terminate enzymatic hydrolysis, obtain apricot Bao
Mushroom digests slurries；
(4) the Pleurotus eryngii degradation in step (3) is filtered after taking filtrate, rotary evaporation to be concentrated to the 1/4 of former filtrate volume,
Dehydrated alcohol is added with the volume ratio of 1:3, at 4 DEG C of constant temperature overnight, is centrifuged 20min at 4000r/min, discards supernatant liquid,
Precipitating is taken, gained precipitating is cleaned 3 times repeatedly with dehydrated alcohol, and vacuum freeze drying obtains Pleurotus eryngii Thick many candies.
Embodiment 4
A kind of Polysaccharide in Pleurotus eryngii extraction process, the steps include:
(1) after fresh Pleurotus eryngii being cleaned up, cut fritter be put into vacuum oven it is dry, to Pleurotus eryngii perseverance quality,
It is put into pulverizer and crushes, sieving obtains Pleurotus eryngii powder, and the partial size of Pleurotus eryngii powder is 160 mesh, spare；
(2) it is transferred in heatproof container, boils after mixing Pleurotus eryngii powder with 13 times of Pleurotus eryngii powder weight of distilled water
It is cooled to room temperature after boiling 10min；
(3) it is 7 that the coolant liquid in step (2), which adjusts pH value to it, and cellulase and neutral protein are added in coolant liquid
Enzyme, the additive amount of cellulase are the 1% of Pleurotus eryngii powder additive amount weight, and the additive amount of neutral proteinase is Pleurotus eryngii powder
The 0.3% of additive amount weight, after digesting 4h at 55 DEG C of bath temperature, in the case where supersonic frequency is 130kHz, ultrasonic wave extraction 7min,
Interval 12min, at ultrasonic 180kHz, ultrasonic wave extraction 7min, boiling water enzyme deactivation 10min terminate enzymatic hydrolysis, obtain Pleurotus eryngii enzymatic hydrolysis
Slurries；
(4) the Pleurotus eryngii degradation in step (3) is filtered after taking filtrate, rotary evaporation to be concentrated to the 1/3 of former filtrate volume,
Dehydrated alcohol is added with the volume ratio of 1:3, at 4 DEG C of constant temperature overnight, is centrifuged 20min at 4000r/min, discards supernatant liquid,
Precipitating is taken, gained precipitating is cleaned 3 times repeatedly with dehydrated alcohol, and vacuum freeze drying obtains Pleurotus eryngii Thick many candies.
One embodiment of the present invention has been described in detail above, but the content is only preferable implementation of the invention
Example, should not be considered as limiting the scope of the invention.It is all according to all the changes and improvements made by the present patent application range
Deng should still be within the scope of the patent of the present invention.
Claims (6)
1. a kind of Polysaccharide in Pleurotus eryngii extraction process, which is characterized in that the steps include:
(1) it after cleaning up fresh Pleurotus eryngii, cuts fritter and is put into drying in vacuum oven, to Pleurotus eryngii perseverance quality, be put into
It is crushed in pulverizer, sieving obtains Pleurotus eryngii powder, spare；
(2) it is transferred in heatproof container after mixing Pleurotus eryngii powder with 10-20 times of Pleurotus eryngii powder weight of distilled water, boiling water
It is cooled to room temperature after boiling 10min；
(3) it is 5-7 that the coolant liquid in step (2), which adjusts pH value to it, and cellulase and neutral proteinase are added in coolant liquid,
After a certain period of time, in the case where supersonic frequency is 120-140kHz, ultrasonic wave extraction 4-7min, interval 8-12min are surpassing water enzyme digestion
Under sound 160-180kHz, ultrasonic wave extraction 4-7min, boiling water enzyme deactivation 10min terminate enzymatic hydrolysis, obtain Pleurotus eryngii enzymatic hydrolysis slurries；
(4) the Pleurotus eryngii degradation in step (3) is filtered after taking filtrate, rotary evaporation to be concentrated to the 1/4-1/3 of former filtrate volume,
Dehydrated alcohol is added with the volume ratio of 1:3, at 4 DEG C of constant temperature overnight, is centrifuged 20min at 4000r/min, discards supernatant liquid,
Precipitating is taken, gained precipitating is cleaned 3-4 times repeatedly with dehydrated alcohol, and vacuum freeze drying obtains Pleurotus eryngii Thick many candies.
2. Polysaccharide in Pleurotus eryngii extraction process according to claim 1, which is characterized in that Pleurotus eryngii in the step (1)
The partial size of powder is 100-200 mesh.
3. Polysaccharide in Pleurotus eryngii extraction process according to claim 1, which is characterized in that cellulose in the step (3)
The additive amount of enzyme is the 0.8-1% of Pleurotus eryngii powder additive amount weight in step (2).
4. Polysaccharide in Pleurotus eryngii extraction process according to claim 1, which is characterized in that the neutrality in the step (3)
The additive amount of protease is the 0.1-0.3% of Pleurotus eryngii powder additive amount weight in step (2).
5. Polysaccharide in Pleurotus eryngii extraction process according to claim 1, which is characterized in that water-bath enzyme in the step (3)
The temperature of solution is 40-55 DEG C.
6. Polysaccharide in Pleurotus eryngii extraction process according to claim 1, which is characterized in that water-bath enzyme in the step (3)
The time of solution is 2-4h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910626044.8A CN110372807A (en) | 2019-07-11 | 2019-07-11 | A kind of Polysaccharide in Pleurotus eryngii extraction process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910626044.8A CN110372807A (en) | 2019-07-11 | 2019-07-11 | A kind of Polysaccharide in Pleurotus eryngii extraction process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110372807A true CN110372807A (en) | 2019-10-25 |
Family
ID=68252816
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910626044.8A Pending CN110372807A (en) | 2019-07-11 | 2019-07-11 | A kind of Polysaccharide in Pleurotus eryngii extraction process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110372807A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115381087A (en) * | 2022-06-22 | 2022-11-25 | 深圳市维龄可伴生物科技有限公司 | Efficient extraction process and application of needle mushroom powder |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006119782A1 (en) * | 2005-05-10 | 2006-11-16 | Mubarak City For Scientific Research And Technology Applications | Anticancer effect of polysaccharides occuring in pleurotus ostreatus mycelia |
| CN104448022A (en) * | 2014-12-11 | 2015-03-25 | 丽水学院 | Extraction method of pleurotus eryngii polysaccharide and application thereof |
| CN109134691A (en) * | 2018-11-16 | 2019-01-04 | 绩溪县徽菜宝生物科技有限公司 | A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii |
-
2019
- 2019-07-11 CN CN201910626044.8A patent/CN110372807A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006119782A1 (en) * | 2005-05-10 | 2006-11-16 | Mubarak City For Scientific Research And Technology Applications | Anticancer effect of polysaccharides occuring in pleurotus ostreatus mycelia |
| CN104448022A (en) * | 2014-12-11 | 2015-03-25 | 丽水学院 | Extraction method of pleurotus eryngii polysaccharide and application thereof |
| CN109134691A (en) * | 2018-11-16 | 2019-01-04 | 绩溪县徽菜宝生物科技有限公司 | A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii |
Non-Patent Citations (2)
| Title |
|---|
| 王雅等: ""超声波协同复合酶法提取杏鲍菇多糖及抗氧化活性的研究"", 《食品工业》 * |
| 苗敬芝等: ""超声协同酶法提取杏鲍菇中可溶性膳食纤维及抗氧化活性研究"", 《食品工业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115381087A (en) * | 2022-06-22 | 2022-11-25 | 深圳市维龄可伴生物科技有限公司 | Efficient extraction process and application of needle mushroom powder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102643368B (en) | Method for synchronously extracting taurine, chitin and polypeptide from shrimp heads and shrimp leftovers | |
| CN109645399A (en) | Sweet potato whole powder prevent-browning processing method | |
| CN108753894A (en) | A kind of extracting method of American Ginseng peptide | |
| CN109134691A (en) | A kind of method of high efficiency extraction Polysaccharide in Pleurotus eryngii | |
| CN106666640A (en) | Natural plant nutritional salt capable of replacing sodium chloride and process for preparing natural plant nutritional salt | |
| KR102332053B1 (en) | Method for producing functional salt | |
| CN106723026A (en) | A kind of Barley Greeg and its preparation technology | |
| CN102839047A (en) | Method for extracting fish oil and producing taurine seafood condiments by using hairtail and leftovers of hairtail | |
| CN110372807A (en) | A kind of Polysaccharide in Pleurotus eryngii extraction process | |
| CN106832021A (en) | A kind of preparation method of Chinese cassia tree slag Thick many candies | |
| CN107267571A (en) | A kind of extracting method of rice bran polysaccharide | |
| CN103621929A (en) | Method for preparing sugar-free and smell-free pumpkin powder | |
| CN105433293A (en) | Minced euphausia superba rich in phospholipid and preparing method thereof | |
| CN110915850A (en) | Method for simultaneously preparing fishy smell removed fishbone powder and antioxidant by utilizing fishbone | |
| KR101455209B1 (en) | seasonings containing collagen by using byproduct of dried yellow pollack | |
| CN104805162A (en) | Preparation method of pleurotus citrinopileatus amino acid powder | |
| CN107801974A (en) | A kind of preparation method of sea cucumber peptide salt | |
| CN107232572A (en) | A kind of method that utilization needle mushroom pin produces food-grade tasty agents | |
| CN107164441A (en) | A kind of method for improving dried sea-cucumber albumen and polysaccharide extract rate | |
| CN106632208A (en) | Extraction method for anthocyanin from purple sweet potato | |
| CN106892989A (en) | A kind of blackberry, blueberry preparation method | |
| CN109123062A (en) | A kind of preparation method and application of peptide calcium chelate | |
| CN104770744A (en) | Bamboo sprout amino acid preparation method and application thereof | |
| CN104131060B (en) | Corbicula fluminea anti-oxidative peptide and preparation method thereof | |
| CN108925741A (en) | A kind of method of ultrasonic wave added subcritical abstraction preparation squid albumen powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191025 |