CN104805162A - Preparation method of pleurotus citrinopileatus amino acid powder - Google Patents
Preparation method of pleurotus citrinopileatus amino acid powder Download PDFInfo
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- CN104805162A CN104805162A CN201510140389.4A CN201510140389A CN104805162A CN 104805162 A CN104805162 A CN 104805162A CN 201510140389 A CN201510140389 A CN 201510140389A CN 104805162 A CN104805162 A CN 104805162A
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- amino acid
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- acid powder
- yuhuang mushroom
- enzymolysis
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Abstract
The invention provides a preparation method of pleurotus citrinopileatus amino acid powder and belongs to the technical field of preparation of amino acid. The preparation method of the pleurotus citrinopileatus amino acid powder comprises washing, pulping and enzymolysis filtration, wherein enzymatic hydrolysate is subjected to filtration with a 100-mesh sieve, ultrafiltration, flash evaporation concentration, spray drying and the like. With adoption of techniques of breaking assisted by ultrasonic waves as well as enzymolysis, breaking of cell walls of pleurotus citrinopileatus is facilitated, so that nutritional ingredients can be dissolved out sufficiently, protein in pleurotus citrinopileatus can be sufficiently hydrolyzed to amino acid and small molecule peptides, and accordingly, the protein in pleurotus citrinopileatus can be conveniently absorbed by a human body. With adoption of the flash evaporation concentration and spray drying techniques, microcapsulated pleurotus citrinopileatus amino acid powder is free of residual solvents, good in embedding effect and higher in yield.
Description
Technical field
The present invention relates to amino acid whose preparing technical field, particularly relate to a kind of preparation method of YUHUANG MUSHROOM amino acid powder.
Background technology
YUHUANG MUSHROOM is a kind of edible and medicinal fungi of delicious food, containing multiple nutritional components such as rich in protein, amino acid and VITAMIN.According to surveying and determination, in the dry sporophore of YUHUANG MUSHROOM, crude protein content is 41.5%, crude fat is 3.8%, ash content is 7.8%, robust fibre is 8.4%, and total amino acid content is 28.65%, compared with edible protein in 3, L-glutamic acid, Methionin, arginine relative content are abundanter, and total free aminoacids accounts for 48% of total amino acid.12 kinds of elements such as P, K, Mg, Fe, Zn, Se, Ge are rich in YUHUANG MUSHROOM sporophore.
Amino acid classes contained in YUHUANG MUSHROOM is complete, the content of indispensable amino acid is higher, account for 41.54% of total amino acid content, wherein, L-glutamic acid, Methionin, arginic content are respectively 7.17g/100g, 1.98g/100g and 2.19g/100g, compared with other edible mushroomss, all there is obvious advantage, and due to the primary amino acid that this few seed amino acid is all edible mushrooms flavor, so YUHUANG MUSHROOM not only delicious flavour, and nutritious, comprehensive.
At present, in document both domestic and external, YUHUANG MUSHROOM polysaccharide mostly is to the research of YUHUANG MUSHROOM functional component, and the extraction process of YUHUANG MUSHROOM aminoacid component be have not been reported.
Summary of the invention
The problem to be solved in the present invention is little to the utilization of aminoacid component in YUHUANG MUSHROOM in existing technology, can not make full use of the nutritive value of YUHUANG MUSHROOM.
For solving the problems of the technologies described above, the technical solution used in the present invention is: the preparation method providing a kind of YUHUANG MUSHROOM amino acid powder.The preparation method of described YUHUANG MUSHROOM amino acid powder, comprises the following steps:
(1) clean, pull an oar: YUHUANG MUSHROOM is washed away the impurity such as silt in clear water, is cut into 1 ~ 3cm segment afterwards, adding weight is pull an oar after the pure water of YUHUANG MUSHROOM 8 ~ 15 times;
(2) enzymolysis: adopt endopeptidase and peptide ending enzyme to carry out enzymolysis to YUHUANG MUSHROOM by product slurry;
(3) filter: enzymolysis solution 100 mesh sieves are filtered;
(4) ultrafiltration: the enzymolysis solution after filtering is carried out ultrafiltration, and aperture is 8 ~ 12nm, and pressure is 0.08 ~ 0.12MPa;
(5) flash concentration: the filtrate that step (4) obtains is carried out flash concentration, makes the solid content of concentrated solution be 15 ~ 24% (weight);
(6) spraying dry: the concentrated solution that step (5) obtains is carried out spraying dry, obtains YUHUANG MUSHROOM amino acid powder.
Further, individual in described step (1), after cleaning, precook YUHUANG MUSHROOM in the water of 90 ~ 95 DEG C 5 ~ 8min, is cut into 1 ~ 3cm segment more afterwards.
Further, in described step (1), after making beating, ultrasonication 8 ~ 12min is carried out to slurries,
Preferably, the frequency of described ultrasonication is 60 ~ 100Hz.
Further, during enzymolysis, first adjusting pH is 4.0 ~ 4.5, and temperature is 45 ~ 55 DEG C, adds endopeptidase and carries out enzymolysis, and adjusting pH is afterwards 5.0 ~ 5.5, and temperature is 15 ~ 55 DEG C, adds peptide ending enzyme and carries out enzymolysis.
Preferably, the enzymolysis time 3 ~ 5h of endopeptidase, the enzymolysis time of peptide ending enzyme is 5 ~ 7h.
Further, in step (4), the condition of ultrafiltration is film water flux is 30L/ (m
2h).
Further, in described step (5), the vacuum tightness of flash concentration is 82 ~ 88kPa.
Preferably, in described step (6), spraying dry is using gum arabic and maltodextrin as wall material, wherein gum arabic: maltodextrin is for 1:1.5 ~ 2; The mass ratio of core and wall material is 4:6.
Further, in described step (6), spray-dired condition is inlet temperature is 150 ~ 160 DEG C, and air outlet temperature is 75 ~ 90 DEG C.
The advantage that the present invention has and positively effect are: the present invention, by utilizing ultrasonic assistant fragmentation and zymolysis technique, is conducive to YUHUANG MUSHROOM cell walls broken, makes the abundant stripping of nutritive ingredient, and make the protein in YUHUANG MUSHROOM fully be hydrolyzed to amino acid and small-molecular peptides; By utilizing ultra-filtration technique to make amino acid and small-molecular peptides and other compositions be separated, the amino acid purity obtained is high; Simultaneously by flash concentration and spray drying technology, make micro encapsulation YUHUANG MUSHROOM amino acid powder no solvent residue, embed effective, yield is higher.
Embodiment
For a better understanding of the present invention, below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment 1
A preparation method for YUHUANG MUSHROOM amino acid powder, comprises following step:
(1) clean, precook: YUHUANG MUSHROOM is washed away the impurity such as silt in pure water, be cut into the segment of 1 ~ 3cm afterwards, precook 5min in the water of 90 DEG C, pulled out.
(2) pull an oar: being pulled an oar by the YUHUANG MUSHROOM segment obtained in 8 times of pure water to YUHUANG MUSHROOM weight and step (1), is carry out ultrasonic assistant fragmentation, ultrasonic time 8min under the condition of 60 ~ 100Hz afterwards in frequency.
(3) enzymolysis: adopt endopeptidase and peptide ending enzyme to carry out enzymolysis to YUHUANG MUSHROOM by product slurry.First adjusting pH is 4.0, and temperature is 45 DEG C, adds endopeptidase and carries out enzymolysis, enzymolysis time 3h; Adjusting pH is afterwards 5.0, and temperature is 15 DEG C, adds peptide ending enzyme and carries out enzymolysis, and enzymolysis time is 5h.
(4) filter: enzymolysis solution 100 mesh sieves are filtered.
(5) ultrafiltration: filtrate step (4) obtained is sent in ultrafiltration apparatus and carried out ultrafiltration, and aperture is 8nm, and working pressure is 0.08MPa, and film water flux is 30L/ (m
2h), the small-molecule substance such as amino acid, polypeptide and the macromolecular substance such as polysaccharide, protein is made to be separated.
(6) flash concentration: the filtrate of collecting step (5) carries out flash concentration, and vacuum tightness is 82kPa, makes the solid content of concentrated solution be 16%.
(7) spraying dry: using gum arabic and maltodextrin as wall material, wherein gum arabic: maltodextrin for 1:1.5, core: wall material is 4:6, carry out spraying dry, wherein, inlet temperature is 150 DEG C, air outlet temperature is 75 DEG C, finally obtains YUHUANG MUSHROOM amino acid powder.
Embodiment 2
A preparation method for YUHUANG MUSHROOM amino acid powder, comprises following step:
(1) clean, precook: YUHUANG MUSHROOM is washed away the impurity such as silt in clear water, be cut into the segment of 1 ~ 3cm afterwards, precook 6min in the water of 95 DEG C, pulled out.
(2) pull an oar: being pulled an oar by the YUHUANG MUSHROOM segment obtained in 12 times of pure water to YUHUANG MUSHROOM weight and step (1), is carry out ultrasonic assistant fragmentation, ultrasonic time 10min under the condition of 60 ~ 100Hz afterwards in frequency.
(3) complex enzyme zymohydrolysis: adopt endopeptidase and peptide ending enzyme to carry out enzymolysis to YUHUANG MUSHROOM by product slurry.First adjusting pH is 4.5, and temperature is 50 DEG C, adds endopeptidase and carries out enzymolysis, enzymolysis time 4h; Adjusting pH is afterwards 5.5, and temperature is 30 DEG C, adds peptide ending enzyme and carries out enzymolysis, and enzymolysis time is 6h.
(4) filter: enzymolysis solution 100 mesh sieves are filtered.
(5) ultrafiltration: filtrate step (4) obtained is sent in ultrafiltration apparatus and carried out ultrafiltration, and aperture is 10nm, and working pressure is 0.10MPa, and film water flux is 30L/ (m
2h), the small-molecule substance such as amino acid, polypeptide and the macromolecular substance such as polysaccharide, protein is made to be separated.
(5) flash concentration: collection filtrate portion enters in flash concentration equipment and carries out flash concentration, and vacuum tightness is 85kPa, makes the solid content of concentrated solution be 20%.
(6) spraying dry: using gum arabic and maltodextrin as wall material, wherein gum arabic: maltodextrin for 1:1.8, core: wall material is 4:6, carry out spraying dry, wherein, inlet temperature is 155 DEG C, air outlet temperature is 82 DEG C, finally obtains YUHUANG MUSHROOM amino acid powder.
Embodiment 3
A preparation method for YUHUANG MUSHROOM amino acid powder, comprises following step:
(1) clean, precook: YUHUANG MUSHROOM is washed away the impurity such as silt in pure, be cut into the segment of 1 ~ 3cm afterwards, precook 8min in the water of 95 DEG C, pulled out.
(2) pull an oar: being pulled an oar by the YUHUANG MUSHROOM segment obtained in 15 times of pure water to YUHUANG MUSHROOM weight and step (1), is carry out ultrasonic assistant fragmentation, ultrasonic time 12min under the condition of 60 ~ 100Hz afterwards in frequency.
(3) complex enzyme zymohydrolysis: adopt endopeptidase and peptide ending enzyme to carry out enzymolysis to YUHUANG MUSHROOM by product slurry.First adjusting pH is 4.5, and temperature is 55 DEG C, adds endopeptidase and carries out enzymolysis, enzymolysis time 5h; Adjusting pH is afterwards 5.5, and temperature is 55 DEG C, adds peptide ending enzyme and carries out enzymolysis, and enzymolysis time is 7h.
(4) filter: enzymolysis solution 100 mesh sieves are filtered.
(5) ultrafiltration: filtrate step (4) obtained is sent in ultrafiltration apparatus and carried out ultrafiltration, and aperture is 12nm, and working pressure is 0.12MPa, and film water flux is 30L/ (m
2h), the small-molecule substance such as amino acid, polypeptide and the macromolecular substance such as polysaccharide, protein is made to be separated.
(5) flash concentration: collection filtrate portion enters in flash concentration equipment and carries out flash concentration, and vacuum tightness is 88kPa, makes the solid content of concentrated solution be 24%.
(6) spraying dry: using gum arabic and maltodextrin as wall material, wherein gum arabic: maltodextrin for 1:2, core: wall material is 4:6, carry out spraying dry, wherein, inlet temperature is 160 DEG C, air outlet temperature is 90 DEG C, finally obtains YUHUANG MUSHROOM amino acid powder.
Above embodiments of the invention have been described in detail, but described content being only preferred embodiment of the present invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the scope of the invention change and improve, and all should still belong within this patent covering scope.
Claims (10)
1. a preparation method for YUHUANG MUSHROOM amino acid powder, is characterized in that: comprise the following steps:
(1) clean, pull an oar: YUHUANG MUSHROOM is washed away the impurity such as silt in clear water, is cut into 1 ~ 3cm segment afterwards, adding weight is pull an oar after the pure water of YUHUANG MUSHROOM 8 ~ 15 times;
(2) enzymolysis: adopt endopeptidase and peptide ending enzyme to carry out enzymolysis to YUHUANG MUSHROOM by product slurry;
(3) filter: enzymolysis solution 100 mesh sieves are filtered;
(4) ultrafiltration: the enzymolysis solution after filtering is carried out ultrafiltration, and aperture is 8 ~ 12nm, and pressure is 0.08 ~ 0.12MPa;
(5) flash concentration: the filtrate that step (4) obtains is carried out flash concentration, makes the solid content of concentrated solution be 15 ~ 24% (weight);
(6) spraying dry: the concentrated solution that step (5) obtains is carried out spraying dry, obtains YUHUANG MUSHROOM amino acid powder.
2. the preparation method of YUHUANG MUSHROOM amino acid powder according to claim 1, is characterized in that: individual in described step (1), after cleaning, precook YUHUANG MUSHROOM in the water of 90 ~ 95 DEG C 5 ~ 8min, is cut into 1 ~ 3cm segment more afterwards.
3. the preparation method of YUHUANG MUSHROOM amino acid powder according to claim 1 and 2, is characterized in that: in described step (1), after making beating, carry out ultrasonication 8 ~ 12min to slurries.
4. the preparation method of YUHUANG MUSHROOM amino acid powder according to claim 3, is characterized in that: the frequency of described ultrasonication is 60 ~ 100Hz.
5. the preparation method of YUHUANG MUSHROOM amino acid powder according to claim 1, is characterized in that: during enzymolysis, and first adjusting pH is 4.0 ~ 4.5, temperature is 45 ~ 55 DEG C, adds endopeptidase and carries out enzymolysis, and adjusting pH is afterwards 5.0 ~ 5.5, temperature is 15 ~ 55 DEG C, adds peptide ending enzyme and carries out enzymolysis.
6. the preparation method of YUHUANG MUSHROOM amino acid powder according to claim 5, is characterized in that: the enzymolysis time 3 ~ 5h of endopeptidase, and the enzymolysis time of peptide ending enzyme is 5 ~ 7h.
7. the preparation method of YUHUANG MUSHROOM amino acid powder according to claim 1, is characterized in that: in step (4), and the condition of ultrafiltration is film water flux is 30L/ (m
2h).
8. the preparation method of YUHUANG MUSHROOM amino acid powder according to claim 1, is characterized in that: in described step (5), and the vacuum tightness of flash concentration is 82 ~ 88kPa.
9. the preparation method of YUHUANG MUSHROOM amino acid powder according to claim 1, is characterized in that: in described step (6), and spraying dry is using gum arabic and maltodextrin as wall material, wherein gum arabic: maltodextrin is for 1:1.5 ~ 2; The mass ratio of core and wall material is 4:6.
10. the preparation method of YUHUANG MUSHROOM amino acid powder according to claim 9, is characterized in that: in described step (6), and spray-dired condition is inlet temperature is 150 ~ 160 DEG C, and air outlet temperature is 75 ~ 90 DEG C.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109527505A (en) * | 2018-12-25 | 2019-03-29 | 大连工业大学 | A kind of preparation method of edible mushroom seasoning product |
| CN110140802A (en) * | 2019-06-18 | 2019-08-20 | 天津市食品研究所有限公司 | A kind of preparation method of the modified processing tasty agents of mushroom stems |
| CN111955608A (en) * | 2020-08-26 | 2020-11-20 | 武汉轻工大学 | Composite amino acid microcapsule and preparation method thereof |
| CN112153902A (en) * | 2018-03-01 | 2020-12-29 | 463Ip合作有限责任公司 | Microencapsulated product, transparent solution thereof and preparation method |
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2015
- 2015-03-27 CN CN201510140389.4A patent/CN104805162A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112153902A (en) * | 2018-03-01 | 2020-12-29 | 463Ip合作有限责任公司 | Microencapsulated product, transparent solution thereof and preparation method |
| JP2021515595A (en) * | 2018-03-01 | 2021-06-24 | ４６３アイピー パートナーズ， エルエルシー | Microencapsulated product, its clear solution, and method of preparation |
| JP7386817B2 (en) | 2018-03-01 | 2023-11-27 | ４６３アイピー パートナーズ， エルエルシー | Microencapsulated products, their clear solutions, and preparation methods |
| CN109527505A (en) * | 2018-12-25 | 2019-03-29 | 大连工业大学 | A kind of preparation method of edible mushroom seasoning product |
| CN110140802A (en) * | 2019-06-18 | 2019-08-20 | 天津市食品研究所有限公司 | A kind of preparation method of the modified processing tasty agents of mushroom stems |
| CN111955608A (en) * | 2020-08-26 | 2020-11-20 | 武汉轻工大学 | Composite amino acid microcapsule and preparation method thereof |
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