CN103549110A - Method for extracting water-soluble rice bran proteins - Google Patents
Method for extracting water-soluble rice bran proteins Download PDFInfo
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- CN103549110A CN103549110A CN201310536586.9A CN201310536586A CN103549110A CN 103549110 A CN103549110 A CN 103549110A CN 201310536586 A CN201310536586 A CN 201310536586A CN 103549110 A CN103549110 A CN 103549110A
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- rice bran
- extracting method
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- soluble rice
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Abstract
The invention discloses a method for extracting water-soluble rice bran proteins. The method comprises the following steps: (1) preprocessing degreased rice bran; (2) ultrasonically processing; (3) carrying out protease hydrolysis; (4) carrying out centrifugal separation, and washing sediments; and (5) carrying out freeze-drying. The rice bran proteins extracted by using the method are relatively high in yield, high in purity and good in water solubility.
Description
Technical field
The present invention relates to a kind of extracting method of rice bran protein, especially relate to a kind of method that adopts ultrasonic wave auxilin enzyme to extract soluble rice bran.
Background technology
China is rice big producing country, producing rice per year reaches more than 1.8 hundred million tons, and rice bran is as rice processed side product, annual production is 10 million ton (Yao Huiyuan also, Zhou Sumei, Wang Li. the exploitation [J] of rice bran and rice bran protein matter. Wuxi light industry college journal, 2002,21 (3): 312 ~ 316).Rice bran is a kind of resource that deep development is worth that has, especially rice bran protein has low-allergen, its amino acid forms approaches with FAO/WHO recommendation pattern, be of high nutritive value, can compare favourably with animal protein, can be widely used in exploitation (Chen Yiyong, the Wang Wei of infant food, Shen Zonggen, Deng. rice bran and rice bran protein deep development present situation [J]. grain processing, 2006,3 (5): 24 ~ 25).
At present, the extracting method of rice bran protein mainly contain alkaline process, Physical, enzyme process etc. (Zhu Lei, Wang Xuede ，Yu Xin state etc., the comprehensive study progress [J] of rice bran protein. Chinese oil, 2013(2): 81-83).The easy sex change of rice bran protein and recovery rate prepared by alkali process are low, and Physical recovery rate is low, equipment investment higher (Jiang Aizhi, Wang Yan. the progress of rice bran protein extracting method [J]. processing of farm products-innovation version, 2009 (10): 35-37).Enzyme process mainly adopts protease, in order to reach the functional and trophism that rice bran protein is good, when using protease, need to control lower degree of hydrolysis (DH), the difficulty of extraction process greatly increases (Ma Haile, Zhang Lianbo. the impulse ultrasound auxiliary extraction technology [J] of rice bran protein. Jiangsu University's journal (natural science edition), 2006 (1): 14 ~ 17).The rice bran protein that above three kinds of methods are extracted is water-soluble poor, be unfavorable for digesting and assimilating of human body, and recovery rate is low.
Summary of the invention
Technical problem to be solved by this invention is, overcomes the deficiencies in the prior art, take defatted rice bran as raw material, and the extracting method of the soluble rice bran that a kind of preparation efficiency is high is provided, and gained rice bran protein purity is high, water-soluble good.
The technical scheme that the present invention solves its technical problem employing is that a kind of extracting method of soluble rice bran, comprises the following steps:
(1) defatted rice bran pretreatment: defatted rice bran is crossed 20-100 mesh sieve, adds distilled water according to solid-liquid ratio 1:10-40 (g:mL) (preferably 1g:20mL), adopts delicatessen food level alkali salt that pH is adjusted to 7-10, obtains defatted rice bran aqueous mixtures;
(2) ultrasonic wave is processed: the defatted rice bran aqueous mixtures of step (1) gained is placed in to beaker, and beaker is placed in to water-bath, bath temperature keeps 10 ~ 30 ℃ (preferably 20 ℃), ultrasonic pulse is processed, ultrasonic power is the preferred 600W of 400-1200W(), pulse working time 1-4 s, pulse interval 1-4 s, total processing time is the preferred 60min of 30 ~ 100 min(), this process will adopt pH meter Real-Time Monitoring to make pH be stabilized in 7-10;
(3) protease hydrolytic: first adopt the pH of the mixture after food grade hydrochloric acid is processed step (2) to be adjusted to 5-9, add the protease of the 2-4% that is equivalent to defatted rice bran material quality to carry out enzyme digestion reaction, hydrolysis temperature is 25-60 ℃ (preferably 45 ℃), and the time is 2-4 h;
(4) centrifugation, washing of precipitate: the solution obtaining after step (3) reaction is gone out after enzyme 5-20 min at 70-100 ℃, centrifugal 10-20 min under 2500 ~ 3500 r/min, collect supernatant, adopt food grade salt acid for adjusting pH to 3.0-5.5, again centrifugal, gained precipitation is soluble rice bran;
(5) freeze drying: the albumen precipitation soluble rice bran that step (4) is obtained carries out freeze drying processing; Pre-freeze speed is 1-5 ℃/min, and hothouse vacuum is 50 ~ 500 Pa, and in the time of-30 ~-10 ℃, pre-freeze 10-30 min, is dried to moisture≤8 %, obtains.
Further, the food grade alkaline salts in described step (1) is sodium carbonate.
Further, the protease in described step (3) is the bent protease of rice, and the enzyme work of described protease is 1 * 10
4-4 * 10
4u/g.
Rice bran protein recovery rate and functional characteristic evaluation method:
1. the mensuration of rice bran protein content and recovery rate
Protein content employing micro-Kjeldahl (AOAC. Official methods of analysis [M]. 15th ed. Washington D C:Association of Official Analytical Chemists, 1990) mensuration (conversion coefficient is 5.95); Recovery rate E=(W
1/ W
0) * 100%, W in formula
1for protein quality in extract (g), W
0for protein quality in rice bran (g).
2. the water-soluble evaluation method of rice bran protein
Solubility with rice bran protein represents, in 50mL ultra-pure water, add the tested rice bran protein quality sample of 1g, in 25 ℃ of thermostat water baths, be incubated, and use agitator to stir 1h, suspension centrifugal (3000r/min) 20min, in supernatant, protein content is measured with biuret method.Be calculated as follows dissolubility:
The present invention's ultrasonic wave auxilin enzyme extracts rice bran protein and has been coupled alkaline process, Physical, enzyme method technique, greatly improved extraction efficiency, and the rice bran protein extracting is water-soluble is better than the water-soluble of rice bran protein that alone alkaline process, Physical or enzyme method technique make.
The specific embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
The extracting method of the soluble rice bran of the present embodiment, comprises the steps:
(1) defatted rice bran pretreatment: defatted rice bran is crossed to 40 mesh sieves, take defatted rice bran 100g, the defatted rice bran that will sieve packs in beaker, add 2L distilled water, use FE20 laboratory pH meter to measure the pH of solution, and adopt the food-grade sodium carbonate liquor of 1mol/L that pH is adjusted to 9, obtain defatted rice bran aqueous mixtures;
(2) ultrasonic wave is processed: the beaker that defatted rice bran aqueous mixtures is housed is placed in to DZKW-4 electronic thermostatic water-bath, bath temperature is stabilized in 20 ℃, then ultrasonic probe is put into beaker, carry out impulse ultrasound extraction, ultrasonic power is 500W, impulse ultrasound interval 2s, pulse working time 2s, extraction time is 60min; Want during this time the pH value of the real time measure solution, make its maintenance be stabilized in pH=9;
(3) protease hydrolytic: after ultrasonic wave is processed, utilize the pH of the mixed solution after 1mol/L hydrochloric acid is processed step (2) to be adjusted to 7.5, add the bent protease of rice of 2g to react, water-bath is stable is controlled at 40 ℃, and the reaction time is 2h;
(4) centrifugation, washing of precipitate: after enzymolysis process finishes, bath temperature is adjusted to 80 ℃, keeps 10min, the solution enzyme that goes out is processed, then with RJ-TDL-40B low speed desk centrifuge, solution is carried out to centrifugal treating, rotating speed is controlled at 3000r/min, time 10min, after centrifugal end, collect supernatant, adopt 1mol/L hydrochloric acid that supernatant is regulated to pH to 4.5, albumen is precipitated, and again carry out centrifugal treating, gained precipitation is soluble rice bran;
(5) freeze drying: the albumen precipitation soluble rice bran that step (4) is obtained carries out freeze drying processing, pre-freeze speed is 3 ℃/min, and hothouse vacuum is 200 Pa, and in the time of-20 ℃, pre-freeze 20 min are dry, and being dried to moisture is 5%, obtains.
Ultrasound wave auxiliary enzyme method is extracted the evaluation of soluble rice bran: adopt micro-Kjeldahl, the recovery rate that calculates rice bran protein is 79.21%, and purity is 80.34%, and the rice bran protein dissolubility of gained is 99.13%.
Embodiment 2
The extracting method of the soluble rice bran of the present embodiment, comprises the following steps:
(1) defatted rice bran pretreatment: defatted rice bran is crossed to 60 mesh sieves, take the defatted rice bran 200g that sieves, the defatted rice bran that will sieve packs in beaker, add 2L distilled water, adopt pH meter to measure the pH of solution, and adopt the food sodium carbonate liquor of 1mol/L that pH is adjusted to 8, obtain defatted rice bran aqueous mixtures;
(2) ultrasonic wave is processed: the beaker that step (1) is equipped with to defatted rice bran aqueous mixtures is placed in electronic thermostatic water-bath, bath temperature is stabilized in 25 ℃, then adopt impulse ultrasound to carry out ultrasonic wave assisted extraction, ultrasonic power is 600W, impulse ultrasound time interval 1.8s, pulse working time 2.5s, extraction time is 70min, will be by adopting pH meter Real-Time Monitoring to make pH be stabilized in 8 during ultrasonic processing;
(3) protease hydrolytic: after ultrasonic wave is processed, utilize 1mol/L hydrochloric acid just the pH of the mixture of step (2) after processing be adjusted to 7, add the bent protease of rice of 6g to carry out enzyme digestion reaction, water-bath is stable is controlled at 50 ℃, the reaction time is 1.5h;
(4) centrifugation, washing of precipitate: after enzymolysis process, bath temperature is adjusted to 100 ℃, keeps 8min, the solution enzyme that goes out is processed, then with low speed desk centrifuge, solution is carried out to centrifugal treating, rotating speed is controlled at 2500r/min, and time 15min, after centrifugal end, collect supernatant, adopt 1mol/L hydrochloric acid that supernatant is regulated to pH to 4, albumen is precipitated, and again carry out centrifugal treating; Gained precipitation is soluble rice bran;
(5) freeze drying: the albumen precipitation soluble rice bran after centrifugal is carried out to freeze drying processing, and pre-freeze speed is 2 ℃/min, and hothouse vacuum is 100 Pa, in the time of-20 ℃, pre-freeze 30 min are dry; Being dried to moisture is 6%, obtains.
Ultrasound wave auxiliary enzyme method is extracted the evaluation of soluble rice bran: adopt micro-Kjeldahl, the recovery rate that calculates rice bran protein is 75.45%, and purity is 78.90%, and the rice bran protein dissolubility of gained is 99.20%.
Claims (9)
1. an extracting method for soluble rice bran, is characterized in that, comprises the following steps:
(1) defatted rice bran pretreatment: defatted rice bran is crossed 20-100 mesh sieve, and 1:10-40 adds distilled water according to solid-liquid ratio, adopts alkali salt that pH is adjusted to 7-10, obtains defatted rice bran aqueous mixtures;
(2) ultrasonic wave is processed: the defatted rice bran aqueous mixtures of step (1) gained is placed in to beaker, and beaker is placed in to water-bath, bath temperature keeps 10 ~ 30 ℃, ultrasonic pulse is processed, ultrasonic power is 400-1200W, pulse working time 1-4 s, pulse interval 1-4 s, total processing time is 30 ~ 100 min, and this process will adopt pH meter Real-Time Monitoring to make pH be stabilized in 7-10;
(3) protease hydrolytic: first adopt the pH of the mixture after food grade hydrochloric acid is processed step (2) to be adjusted to 5-9, add the protease of the 2-4% that is equivalent to defatted rice bran material quality to carry out enzyme digestion reaction, hydrolysis temperature is 25-60 ℃, and the time is 2-4 h;
(4) centrifugation, washing of precipitate: the solution obtaining after step (3) reaction is gone out after enzyme 5-20 min at 70-100 ℃, centrifugal 10-20 min under 2500 ~ 3500 r/min, collect supernatant, adopt food grade salt acid for adjusting pH to 3.0-5.5, centrifugal gained precipitation is soluble rice bran again;
(5) freeze drying: the albumen precipitation soluble rice bran that step (4) is obtained carries out freeze drying processing; Pre-freeze speed is 1-5 ℃/min, and hothouse vacuum is 50 ~ 500 Pa, and in the time of-30 ~-10 ℃, pre-freeze 10-30 min, is dried to moisture≤8 %, obtains.
2. the extracting method of soluble rice bran according to claim 1, is characterized in that, in step (1), according to solid-liquid ratio, 1g:20mL adds distilled water.
3. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (2), bath temperature keeps 20 ℃.
4. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (2), ultrasonic power is 600W.
5. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (2), ultrasonic total processing time is 60min.
6. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (3), hydrolysis temperature is 45 ℃.
7. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, the food grade alkaline salts in described step (1) is sodium carbonate.
8. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, the protease in described step (3) is the bent protease of rice.
9. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (3), the enzyme work of described protease is 1 * 10
4-4 * 10
4u/g.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938764A (en) * | 2015-05-25 | 2015-09-30 | 浙江大学 | Method for extracting rice bran proteins |
| CN105475607A (en) * | 2015-11-06 | 2016-04-13 | 武汉轻工大学 | Method for extracting protein from low-temperature rice bran meal |
| CN112481022A (en) * | 2020-12-22 | 2021-03-12 | 霍邱县敏东米业有限公司 | Method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran |
| CN112970933A (en) * | 2021-03-30 | 2021-06-18 | 江西恒顶食品有限公司 | Rice bran protein extraction method |
| CN113176361A (en) * | 2021-06-30 | 2021-07-27 | 中国农业科学院蜜蜂研究所 | Identification method and application of bee pollen allergenic protein |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104938764A (en) * | 2015-05-25 | 2015-09-30 | 浙江大学 | Method for extracting rice bran proteins |
| CN104938764B (en) * | 2015-05-25 | 2020-06-23 | 浙江大学 | Method for extracting rice bran protein |
| CN105475607A (en) * | 2015-11-06 | 2016-04-13 | 武汉轻工大学 | Method for extracting protein from low-temperature rice bran meal |
| CN112481022A (en) * | 2020-12-22 | 2021-03-12 | 霍邱县敏东米业有限公司 | Method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran |
| CN112970933A (en) * | 2021-03-30 | 2021-06-18 | 江西恒顶食品有限公司 | Rice bran protein extraction method |
| CN113176361A (en) * | 2021-06-30 | 2021-07-27 | 中国农业科学院蜜蜂研究所 | Identification method and application of bee pollen allergenic protein |
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| CN103549110B (en) | 2015-06-24 |
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Effective date of registration: 20171206 Address after: Du Zhen Gang village 423000 foot Chenzhou city in Hunan province Suxian District Qi Feng (former Gang Jiao township government office) Patentee after: Hunan Feng Chu Food Technology Co., Ltd. Address before: 410004 Shaoshan South Road, Hunan, No. 498, No. Patentee before: Central South University of Forestry and Technology |
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Address after: 423000 Hunan province Chenzhou city Suxian District Qifeng Village town foot village (formerly Gang Kong township government office building) Patentee after: Hunan Fengchu Food Co.,Ltd. Address before: 423000 Hunan province Chenzhou city Suxian District Qifeng Village town foot village (formerly Gang Kong township government office building) Patentee before: Hunan Feng Chu Food Technology Co.,Ltd. |