CN109438582A - The method of multi-mode ultrasonic wave assisted extraction arrowhead starch - Google Patents
The method of multi-mode ultrasonic wave assisted extraction arrowhead starch Download PDFInfo
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Abstract
The invention discloses the methods of multi-mode ultrasonic wave assisted extraction arrowhead starch, belong to plant amylum extractive technique field.Arrowhead raw material is cleaned up into the water, and carries out peeling impurity elimination processing；Arrowhead raw material is weighed, adds water to be beaten by 1: 1 mass ratio, starch homogenate is put under different ultrasound conditions and carries out ultrasonic pretreatment；Centrifugally operated is then carried out, takes lower sediment after being centrifuged 20min, is put into clean culture dish and is placed in baking oven dry 48h, is ground up, sieved, up to arrowhead starch.The present invention accelerates the movement of solvent molecule in aqueous systems, multiple frequency ultrasonic assisted extraction arrowhead starch, through the cavitation of ultrasonic wave and mechanical oscillation effect, promotes the recovery rate of target product；It is more energy saving using multiple frequency ultrasonic mode.This method operating procedure is simple and easy, is greatly improved the recovery rate of arrowhead starch, and energy consumption is lower, overcomes traditional arrowhead starch isolation in the process using the deficiency of a large amount of lye.
Description
Technical field
The present invention relates to plant amylum extractive technique field, refer in particular to one kind using arrowhead as raw material, it is auxiliary using multi-mode ultrasound
Help the method for extracting arrowhead starch.
Background technique
Arrowhead is also referred to as Herba Passiflorae Cupiformis, it is a kind of aquatic herbaceous plant, and planting patterns is low-lying paddy field.Fresh arrowhead is in
Now long oval or ellipse, length are about 2.2-4.5cm, and diameter is about 1.8-3.2cm.Compendium of Material Medica records kind, I
State is the source area of arrowhead, mainly lives in low-lying paddy field area.Arrowhead is to be distributed in world wide, in China, court
Arrowhead is all edible in the area such as fresh, Japanese, India, Korea.In China, whenever winter-spring season, vegetable supply not in time,
Arrowhead can serve as one of the main vegetables in supply dull season.It is edible that arrowhead acts not only as a kind of vegetables, and its medicine
It is also very high with health value.From the perspective of traditional Chinese medicine, arrowhead have the characteristics that it is sweet in flavor, slightly cold, nontoxic edible,
Has effects that the efficacy of a drug of cool blood, cough-relieving, hemostasis, removing toxic substances, at the same time, the method for eating arrowhead is also more and more.Due to arrowhead
Not only can be as drug, but also can be as food, therefore receive more concerns.Meanwhile medicinal grind is eaten about arrowhead
Studying carefully also becomes more.But so far, we about the processing of arrowhead still based on the preliminary working of the place of production, i.e., directly it is in bulk on
City, or be vacuum-packed and sell after arrowhead is cleaned, the research report of the deep processing about arrowhead is still less.A large number of studies show that
The ingredients such as the starch being rich in arrowhead, therefore novel organic functional food or organic food ingredient can be developed into, it mentions
The added value of high arrowhead.
Statistics can obtain, and global arrowhead annual output is up to 5,000,000 tons.The content of starch is very high in arrowhead, at the same time,
The content of phosphorus is also very high in arrowhead, and with water chestnut compared with lotus root, the carbohydrate content of arrowhead is also very high.But due to arrowhead
Moisture is very high, seasonal very strong, therefore arrowhead is easy to corruption and breaks down.Meanwhile the Exploitation degree of arrowhead is far from enough.Due to kind
Seasonal stronger, some reasons such as single limitation of processing method of aunt itself, the research report of the deep processing about arrowhead is still
It is old less.Studies have shown that the solubility of arrowhead starch is lower.The molecular weight very little of arrowhead amylose and amylopectin, therefore
Its particle is also smaller, and in addition to this, the lipid material of arrowhead also plays apparent inhibition to the dissolution of arrowhead starch granules and makees
With.At the same time, the starch granules in arrowhead is firmly wrapped in interior by protein reticular structure and cement, also gives arrowhead starch
Extraction cause certain difficulty.Therefore, the deep processing of arrowhead starch is restricted, it is difficult to be produced in enormous quantities.
Ultrasonic wave is a kind of sound wave of the vibration frequency higher than 20kHz, it has high-frequency, short wavelength, strong penetrating power, orientation
Property it is good, be easily obtained relatively concentrate sound energy the characteristics of.In the experiment for extracting arrowhead starch, the effect of ultrasonic wave is to arrowhead original
The pretreatment of material.Many experiments show that the solubility of arrowhead starch is lower, and the structure that this is primarily due to arrowhead starch granules is special
It levies and causes difficulty to the extraction of arrowhead starch.Therefore, the deep processing of arrowhead starch is restricted, it is difficult to be produced in enormous quantities.
And ultrasonic wave has very high supersonic frequency, very strong substance penetrability, the very accurately very big spy of azimuthal orientation, energy
Point, lot of documents also indicate that, ultrasonic wave auxiliary extraction technology have the advantages that it is very much, it is such as easy to operate, the time is short, temperature is low
Deng.Therefore, using the technology of ultrasonic wave assisted extraction arrowhead starch, available purity is more preferable, the higher arrowhead of recovery rate is formed sediment
Powder.
Summary of the invention
To solve the above problems, arrowhead cleaning treatment is utilized multi-mode ultrasound assisted extraction technological means system by the invention
Standby arrowhead starch, and study arrowhead starch extraction rate.
The method of multi-mode ultrasonic wave assisted extraction arrowhead starch of the present invention carries out as steps described below:
(1) arrowhead raw material is cleaned up into the water, and carries out peeling impurity elimination processing；
(2) arrowhead raw material is weighed, adds water to be beaten by 1: 1 mass ratio, starch homogenate is put into different multi-mode ultrasounds
Under the conditions of carry out ultrasonic pretreatment；Centrifugally operated is then carried out, lower sediment is taken after being centrifuged 20min, is put into clean culture
It is placed in baking oven dry 48h in ware, is ground up, sieved, up to arrowhead starch.
Wherein carry out ultrasonic pretreatment under the different multi-mode ultrasound conditions to refer to: triple-frequency ultrasonic processing surpasses
Acoustic frequency combination are as follows: 20/40/60kHz, 20/28/40kHz.It is preferred that 20/40/60kHz.
Wherein carry out ultrasonic pretreatment under the different multi-mode ultrasound conditions to refer to: ultrasonic time is respectively
5-30min；Determine that optimal ultrasonic time is 8min.
It wherein carries out ultrasonic pretreatment under the different ultrasound conditions to refer to: ultrasonic power density: 180W-
630W/150mL determines that optimal ultrasonic power density is 540W/150mL.
Wherein the ultrasound works mode of the multi-mode ultrasonic wave is synchronous multifrequency or sequence multifrequency, is preferably synchronized more
Frequently.
The beneficial effects of the present invention are:
The moisture for the raw material arrowhead that the present invention chooses is very high, seasonal very strong, therefore processes not in time, and arrowhead is easy to corruption
Lose rotten, extracting arrowhead starch as raw material using it helps to solve seasonal stronger, the processing method Single-issue of arrowhead itself,
The expansion for being conducive to arrowhead industrial chain extends, while effectively improving the economic value of arrowhead.
The present invention passes through the cavitation and machinery of ultrasonic wave in aqueous systems, multiple frequency ultrasonic assisted extraction arrowhead starch
Effect of vibration accelerates the movement of solvent molecule, promotes the recovery rate of target product；Using multiple frequency ultrasonic mode, reduce reaction
The heating of system, it is more energy saving.This method operating procedure is simple and easy, is greatly improved the recovery rate of arrowhead starch, energy consumption compared with
It is low, traditional arrowhead starch isolation is overcome in the process using the deficiency of a large amount of lye.
Detailed description of the invention
Fig. 1 is multi-mode ultrasonic wave biological processing equipment structure chart of the invention, wherein 1,2,3 be ultrasonic vibrating plate,
4 be liquid holding device, and 5 be water-bath, and 6 be temp probe, and 7 be circulating pump, and 8 be Computerized controller, 9,10,11
For ultrasonic controller.
Specific embodiment
Term as used in the present invention can generally be managed unless otherwise specified by those of ordinary skill in the art
Solution.The present invention is described in further detail below with reference to specific embodiment, and referring to data.Illustrate hereby, these embodiments
It is of the invention solely for the purpose of illustration, rather than limit the scope of the invention in any way.
Fig. 1 is multi-mode ultrasonic wave biological processing equipment of the invention, which is furnished with a Computerized controller 8,
Ultrasound works parameter can be set (when ultrasonic power density, frequency, pulse working time, intermittent time and total processing
Between) three ultrasonic controllers 9,10,11 are controlled respectively, it is separately connected the ultrasonic vibrating plate 1,2,3 of three different frequencies,
The processing of single-frequency/two frequency/tri- frequency ultrasonic wave can be achieved；Solution to be treated is put into liquid holding device
Single-frequency/double frequency/multiple frequency ultrasonic assistance enzymolysis test is carried out in 4, starting circulating pump 7 recycles solution.Pass through water-bath 5
The automatic control of solution temperature is realized with temp probe 6.
(ultrasound is not added) in comparative example 1
Firstly, arrowhead raw material is cleaned up into the water, and carry out peeling impurity elimination processing.Then arrowhead raw material is weighed
75g adds water to be beaten by 1: 1 mass ratio, then carries out centrifugally operated, takes lower sediment after being centrifuged 20min, is put into clean training
It supports and is placed in dry 48h in baking oven in ware, be ground up, sieved, weigh；The recovery rate of arrowhead starch and pure is measured by the following method
Degree.
The measuring method of arrowhead starch extraction rate is as follows in the present invention:
Starch extraction rate is the percentage that pure starch quality accounts for starch quality in raw material in extract.
In formula: XwThe recovery rate (g/100g) of measured arrowhead starch；
The content (g/100g) of the surveyed arrowhead starch of X-；
mwStarch quality (g) in raw material；
M- sample mass (g).
The measuring method of arrowhead purity of starch is as follows in the present invention:
The measurement of arrowhead purity of starch uses enzyme hydrolysis method.Firstly, by by being ultrasonically treated or not being ultrasonically treated kind
Aunt's starch sample is ground to powdered, sieving.Then, 3g arrowhead starch powder is weighed in beaker, sequentially adds ether, second
Alcohol, abundant degreasing desugar.Then, arrowhead starch sample is subjected to constant volume processing, and is gelatinized arrowhead heating starch, after its is cold
Enzyme operation is carried out, arrowhead starch is made to be decomposed into disaccharide, monosaccharide step by step.It is spare finally by its constant volume.
Arrowhead purity of starch is the ratio of pure starch quality and desciccate quality in extracted rear desciccate.
The calculation formula of content of starch in sample:
In formula: XsThe content (g/100g) of surveyed arrowhead starch；
X1The amount (mg) of glucose in alleged sample；
X0Reagent blank value (mg)；
0.9- glucose is transformed to the conversion coefficient of arrowhead starch；
M- sample mass (g).
Comparative example 2-6 (single-frequency and double frequency difference supersonic frequency)
The single-frequency and dual-frequency ultrasonic wave of arrowhead starch pre-process
(1) firstly, arrowhead raw material is cleaned up into the water, and peeling impurity elimination processing is carried out.Then arrowhead original is weighed
Expect 75g, add water to be beaten by 1: 1 mass ratio, starch homogenate is put under different ultrasound conditions and carries out ultrasonic pretreatment.With
After carry out centrifugally operated, take lower sediment after being centrifuged 20min, be put into clean culture dish and be placed in baking oven dry 48h, grind
Mill, sieving, weighing.
(2) experiment of single factor of supersonic frequency: determining that ultrasonic time is 20min, and ultrasonic power density is 180W/150mL,
Ultrasound works model selection synchronizes multifrequency.This supersonic frequency for carrying out the processing of arrowhead starch has 20kHz, 28kHz, 35kHz,
40kHz, 60kHz.Double frequency frequency is freely combined on its basis, total to have 5 kinds of double frequencies.
(3) experiment of single factor of ultrasound works mode: the selected optimal supersonic frequency measured, optimal ultrasonic time and most
Excellent ultrasonic power density, other conditions are constant.The operating mode of ultrasound: synchronous multifrequency, sequence multifrequency.
It finally determines optimal ultrasound condition, calculates the recovery rate and purity of starch.
The measuring method of arrowhead starch extraction rate and purity is referring to comparative example 1.
Comparative example 2-7 preparation process is identical, and only supersonic frequency is different, is specifically shown in Table 1 different ultrasonic power and forms sediment to arrowhead
The influence of powder recovery rate.
Embodiment 1-2 (multifrequency difference supersonic frequency)
The multi-mode ultrasonic pretreatment of arrowhead starch
Firstly, arrowhead raw material is cleaned up into the water, and carry out peeling impurity elimination processing.Then arrowhead raw material is weighed
75g adds water to be beaten by 1: 1 mass ratio, and starch homogenate is put under different ultrasound conditions and carries out ultrasonic pretreatment.Then
Centrifugally operated is carried out, takes lower sediment after being centrifuged 20min, is put into clean culture dish and is placed in baking oven dry 48h, grind
Mill, sieving, weighing.
(1) experiment of single factor of supersonic frequency: determining that ultrasonic time is 20min, and ultrasonic power density is 180W/150mL,
Ultrasound works model selection synchronizes multifrequency.This multiple frequency ultrasonic frequency for carrying out the processing of arrowhead starch has: 20/40/60kHz,
20/28/40kHz。
(2) experiment of single factor of ultrasound works mode: the selected optimal supersonic frequency measured, optimal ultrasonic time and most
Excellent ultrasonic power density, other conditions are constant.The operating mode of ultrasound: synchronous multifrequency, sequence multifrequency.
It finally determines optimal ultrasound condition, calculates the recovery rate and purity of starch.
The measuring method of arrowhead starch extraction rate and purity is referring to comparative example 1.
Embodiment 1-2 preparation process is identical, and only supersonic frequency is different, is specifically shown in Table 1 different ultrasonic power and forms sediment to arrowhead
The influence of powder recovery rate.
Influence of the different supersonic frequencies of table 1 to arrowhead starch extraction rate
Table 1 is arrowhead starch extraction rate measured by multi-frequency ultrasonic treatment front and back.After measured, not ultrasonic arrowhead starch
Recovery rate is 52.68%.When single-frequency is 20kHz, recovery rate 59.60%, than not sonicated arrowhead starch isolation
Rate is higher by 6.92%.Therefore, single-frequency is ultrasonic compared with not ultrasonic control group, starch extraction rate have conspicuousness raising (p <
0.05).Double frequency handles arrowhead starch, and when double frequency is 20/28kHz, the recovery rate of arrowhead starch is 68.80%, more ultrasonic than not
The arrowhead starch of processing improves 9.20%.When double frequency is 20/35kHz, when 20/40kHz, the recovery rate of arrowhead starch is respectively
71.60%, 79.84%；16.12%, 18.92% is improved than not ultrasonic arrowhead starch respectively, is 20/ than double frequency frequency
The arrowhead starch of 28kHz improves l6.58%, and 14.50%.As shown in table 1, double-frequency ultrasound is compared with not ultrasonic arrowhead starch,
Be significantly improved (p < 0.05).From table 1 we it can be found that compared with the arrowhead starch not being ultrasonically treated, three again and again
Rate ultrasound can significantly improve the recovery rate of arrowhead starch.Meanwhile the significant difference very little between triple-frequency ultrasonic, because
The triple-frequency ultrasonic of this two kinds of frequencies is close to the extraction rate impact of arrowhead starch.It can be obtained by table 1, supersonic frequency mentions arrowhead starch
Take the influence of rate are as follows: three frequency > double frequencies > single-frequency.Select optimal frequency: 20/40/60kHz, starch extraction rate: 88.17%.
Influence of the different supersonic frequencies of table 2 to arrowhead purity of starch
| Embodiment | Supersonic frequency (kHz) | Purity (%) |
| Comparative example 1 | Ultrasound is not added | 49.40 |
| Comparative example 2 | 20 | 55.21 |
| Comparative example 3 | 28 | 51.93 |
| Comparative example 4 | 35 | 56.05 |
| Comparative example 5 | 20/28 | 59.03 |
| Comparative example 6 | 20/35 | 65.61 |
| Comparative example 7 | 20/40 | 73.53 |
| Embodiment 1 | 20/40/60 | 75.95 |
| Embodiment 2 | 20/28/40 | 74.60 |
Influence of the different supersonic frequencies of table 2 to arrowhead purity of starch.After measured, ultrasonic arrowhead purity of starch is not
49.40%.When single-frequency is 20kHz, the purity of arrowhead starch is 55.21%, than not sonicated arrowhead starch extraction rate
It is higher by 5.81%.As shown in table 2, the arrowhead starch of single-frequency ultrasonic treatment is compared with the control group not being ultrasonically treated, purity of starch
There is the raising (p < 0.05) of conspicuousness.Analyze double-frequency ultrasound: when double frequency is 20/28kHz, the purity of arrowhead starch is
56.05%, it is compared than not sonicated arrowhead starch and improves 6.65%.When double frequency be 20/35kHz,
When 20/40kHz, the purity of arrowhead starch is respectively 65.61%, 73.53%；Respectively than not ultrasonic arrowhead starch
Purity improves 9.63%, 16.22%, improves 6.58%, 14.50% than the arrowhead starch that double frequency frequency is 20/28kHz.To three
Frequency ultrasound is analyzed: when three supersonic frequencies are 20/28/40kHz, the purity of arrowhead starch is 74.60%, works as triple-frequency ultrasonic
When frequency is 20/40/60kHz, the purity of arrowhead starch is 75.95%.From table 2 we it has also been discovered that, triple-frequency ultrasonic it is aobvious
Sex differernce very little is write, therefore the triple-frequency ultrasonic of two kinds of frequencies is close to the impurities affect of arrowhead starch.Therefore, it can be obtained, be surpassed by table 2
Influence of the acoustic frequency to arrowhead purity of starch are as follows: three frequency > double frequencies > single-frequency.Determine optimal frequency condition are as follows: 20/40/60kHz forms sediment
Powder purity: 75.949%.
Embodiment 3 (multifrequency difference ultrasonic time)
The multi-mode ultrasonic pretreatment of arrowhead starch
Firstly, arrowhead raw material is cleaned up into the water, and carry out peeling impurity elimination processing.Then arrowhead raw material is weighed
75g adds water to be beaten by 1: 1 mass ratio, and starch homogenate is put under different ultrasound conditions and carries out ultrasonic pretreatment.Then
Centrifugally operated is carried out, takes lower sediment after being centrifuged 20min, is put into clean culture dish and is placed in baking oven dry 48h, grind
Mill, sieving, weighing.
(1) experiment of single factor of supersonic frequency: ultrasonic power density is 180W/150mL, and ultrasound works model selection is synchronous
Multifrequency.This carries out the multiple frequency ultrasonic frequency of arrowhead starch processing: 20/40/60kHz.
(2) experiment of single factor of ultrasound works mode: ultrasonic time 5-30min.
It finally determines optimal ultrasound condition, calculates the recovery rate and purity of starch.
The measuring method of arrowhead starch extraction rate and purity is referring to comparative example 1.
Influence of the different ultrasonic times of table 3 to arrowhead starch extraction rate
Table 3 is the arrowhead starch extraction rate that different ultrasonic times is handled.After measured, when ultrasonic 5min, arrowhead starch
Recovery rate is 90.05%；When ultrasonic 8min, recovery rate 95.24%；When ultrasonic 10min, recovery rate 92.73%.With
Ultrasonic arrowhead starch is not compared, and recovery rate has been respectively increased 37.37%, 42.56%, 40.05%, there is apparent raising (p
<0.05).As shown in Table 3, it can be found that the change of ultrasonic time also has a great impact (p < 0.05) to starch extraction rate.Such as
Shown in table 3,8min is the optimal time, and recovery rate is 95.24%.
Influence of the different ultrasonic times of table 4 to arrowhead purity of starch
| Embodiment | Ultrasonic time (min) | Purity (%) |
| Comparative example 1 | Ultrasound is not added | 49.40 |
| Embodiment 3 | 5 | 90.05 |
| Embodiment 3 | 8 | 95.24 |
| Embodiment 3 | 10 | 92.73 |
| Embodiment 3 | 20 | 88.17 |
| Embodiment 3 | 30 | 83.32 |
Table 4 is the arrowhead purity of starch under different ultrasonic times.After measured, the purity of ultrasonic 5min, arrowhead starch is
79.45%；Ultrasonic 8min, purity 83.25%；Ultrasonic 10min, purity 80.80%；As can be seen from Table 4, when
Between experiment of single factor in, arrowhead purity of starch has first to rise drops phenomenon again.Acoustic frequency is determined as on the basis of 20/40/60kHz,
Its dependent variable is controlled, ultrasonic time is only changed, it can be found that ultrasonic time change makes purity of starch, great changes have taken place (p <
0.05).As shown in table 4, when ultrasonic time 8min, 10min, purity of starch is all up to 80% or more.Optimal ultrasonic time is
8min, purity of starch is up to 83.25%.
Embodiment 4 (multifrequency different ultrasonic power density)
The multi-mode ultrasonic pretreatment of arrowhead starch
Firstly, arrowhead raw material is cleaned up into the water, and carry out peeling impurity elimination processing.Then arrowhead raw material is weighed
75g adds water to be beaten by 1: 1 mass ratio, and starch homogenate is put under different ultrasound conditions and carries out ultrasonic pretreatment.Then
Centrifugally operated is carried out, takes lower sediment after being centrifuged 20min, is put into clean culture dish and is placed in baking oven dry 48h, grind
Mill, sieving, weighing.
(1) experiment of single factor of supersonic frequency: ultrasonic time 8min.Ultrasound works model selection synchronizes multifrequency.This
Carry out the multiple frequency ultrasonic frequency of arrowhead starch processing: 20/40/60kHz.
(2) experiment of single factor of ultrasound works mode: ultrasonic power density: 180W/150mL, 270W/150mL, 360W/
150mL, 540W/150mL, 630W/150mL.
It finally determines optimal ultrasound condition, calculates the recovery rate and purity of starch.
The measuring method of arrowhead starch extraction rate and purity is referring to comparative example 1.
Influence of the different ultrasonic times of table 5 to arrowhead starch extraction rate
| Embodiment | Ultrasonic power density | Recovery rate (%) |
| Comparative example 1 | Ultrasound is not added | 52.68 |
| Embodiment 4 | 180W/150mL | 95.24 |
| Embodiment 4 | 270W/150mL | 95.60 |
| Embodiment 4 | 360W/150mL | 96.25 |
| Embodiment 4 | 540W/150mL | 97.07 |
| Embodiment 4 | 630W/150mL | 96.46 |
Table 5 is the recovery rate of the arrowhead starch under different ultrasonic power density.As can be seen from Table 5, in supersonic frequency
It is determined as 20/40/60kHz, ultrasonic time is determined as on the basis of 8min, and it controls its dependent variable and only changes ultrasonic power density,
It can be found that the recovery rate of arrowhead starch has been respectively increased 42.56% compared with not sonicated arrowhead starch,
42.92%, 43.57%, 44.39%, 43.78%, but simultaneously there was no significant difference for the change of power density, starch extraction rate
Range between 95%-98%.Under comparing, optimal ultrasonic power density is 540W/150mL, and starch extraction rate reaches
97.07%.
Influence of the different ultrasonic times of table 6 to arrowhead purity of starch
| Embodiment | Ultrasonic power density | Purity (%) |
| Comparative example 1 | Ultrasound is not added | 49.40 |
| Embodiment 4 | 180W/150mL | 83.25 |
| Embodiment 4 | 270W/150mL | 83.57 |
| Embodiment 4 | 360W/150mL | 84.14 |
| Embodiment 4 | 540W/150mL | 84.86 |
| Embodiment 4 | 630W/150mL | 84.32 |
Table 6 is the arrowhead purity of starch under different ultrasonic power density.As can be seen from Table 6, it is determined as in supersonic frequency
20/40/60kHz, ultrasonic time are determined as on the basis of 8min, control its dependent variable and only change ultrasonic power density, Ke Yifa
Now compared with not ultrasonic arrowhead starch, the arrowhead purity of starch under different capacity density is respectively increased 68.50%~
71.78%.But the purity difference of the arrowhead starch under different capacity density is little.Under comparing, optimal ultrasonic power
Density is 540W/150mL, and purity of starch is up to 84.86%.
Embodiment 5 (multifrequency difference ultrasound works mode)
The multi-mode ultrasonic pretreatment of arrowhead starch
Firstly, arrowhead raw material is cleaned up into the water, and carry out peeling impurity elimination processing.Then arrowhead raw material is weighed
75g adds water to be beaten by 1: 1 mass ratio, and starch homogenate is put under different ultrasound conditions and carries out ultrasonic pretreatment.Then
Centrifugally operated is carried out, takes lower sediment after being centrifuged 20min, is put into clean culture dish and is placed in baking oven dry 48h, grind
Mill, sieving, weighing.
(1) experiment of single factor of supersonic frequency: ultrasonic time 8min.Ultrasonic power density: 540W/150mL.This into
The multiple frequency ultrasonic frequency of row arrowhead starch processing: 20/40/60kHz.
(2) experiment of single factor of ultrasound works mode: ultrasound works model selection synchronous multifrequency, sequence multifrequency.
It finally determines optimal ultrasound condition, calculates the recovery rate and purity of starch.
The measuring method of arrowhead starch extraction rate and purity is referring to comparative example 1.
Influence of the different ultrasound works modes of table 7 to arrowhead starch extraction rate and purity
| Ultrasound works mode | Starch extraction rate (%) | Purity of starch (%) |
| Synchronous multifrequency | 97.07 | 84.86 |
| Sequence multifrequency | 96.10 | 84.01 |
Table 7 is the arrowhead starch extraction rate under different ultrasound modes.It can be obtained by table 7, in optimal supersonic frequency, optimal ultrasound
On the basis of time and optimal ultrasonic power density determine, control in the case that its dependent variable only changes ultrasound works mode,
The arrowhead starch extraction rate that sequence multifrequency is surveyed is 96.10%, and arrowhead starch extraction rate measured by synchronous multifrequency is 97.07%,
By data measured it is found that influence of the change of operating mode to starch extraction rate is smaller, the two in contrast, determines operating mode
For synchronous multifrequency, starch extraction rate 97.07%.The arrowhead purity of starch that sequence multifrequency is surveyed is 84.01%, synchronous multifrequency institute
Measuring arrowhead purity of starch is 84.86%, by data measured it is found that influence of the change of operating mode to purity of starch is smaller,
The two in contrast, determines operating mode for synchronous multifrequency, purity of starch 84.86%.
Claims (8)
1. the method for multi-mode ultrasonic wave assisted extraction arrowhead starch, it is characterised in that carry out as steps described below:
(1) arrowhead raw material is cleaned up into the water, and carries out peeling impurity elimination processing；
(2) arrowhead raw material is weighed, adds water to be beaten by 1: 1 mass ratio, starch homogenate is put into different multi-mode ultrasound conditions
Lower carry out ultrasonic pretreatment；Centrifugally operated is then carried out, lower sediment is taken after being centrifuged 20min, is put into clean culture dish
It is placed in baking oven dry 48h, is ground up, sieved, up to arrowhead starch.
2. the method for multi-mode ultrasonic wave assisted extraction arrowhead starch according to claim 1, it is characterised in that wherein institute
It carries out ultrasonic pretreatment under the different multi-mode ultrasound conditions stated to refer to: triple-frequency ultrasonic processing, supersonic frequency combination are as follows:
20/40/60kHz, 20/28/40kHz.
3. the method for multi-mode ultrasonic wave assisted extraction arrowhead starch according to claim 1, it is characterised in that wherein institute
It carries out ultrasonic pretreatment under the different multi-mode ultrasound conditions stated to refer to: ultrasonic time 5-30min.
4. the method for multi-mode ultrasonic wave assisted extraction arrowhead starch according to claim 1, it is characterised in that wherein institute
Carry out ultrasonic pretreatment under the different ultrasound conditions stated to refer to: ultrasonic power density: 180W-630W/150mL is determined most
Excellent ultrasonic power density is 540W/150mL.
5. the method for multi-mode ultrasonic wave assisted extraction arrowhead starch according to claim 1, it is characterised in that wherein institute
The ultrasound works mode for the multi-mode ultrasonic wave stated is synchronous multifrequency or sequence multifrequency, preferably synchronous multifrequency.
6. the method for multi-mode ultrasonic wave assisted extraction arrowhead starch according to claim 3, it is characterised in that wherein institute
It carries out ultrasonic pretreatment under the different multi-mode ultrasound conditions stated to refer to: ultrasonic time 8min.
7. the method for multi-mode ultrasonic wave assisted extraction arrowhead starch according to claim 4, it is characterised in that wherein institute
Carry out ultrasonic pretreatment under the different ultrasound conditions stated to refer to: ultrasonic power density is 540W/150mL.
8. the method for multi-mode ultrasonic wave assisted extraction arrowhead starch according to claim 1, it is characterised in that wherein institute
The ultrasound works mode for the multi-mode ultrasonic wave stated is synchronous multifrequency.
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| CN110003348A (en) * | 2019-04-30 | 2019-07-12 | 漳州科技职业学院 | A kind of method that pure physics extracts longan seed starch |
| CN111228414A (en) * | 2020-02-02 | 2020-06-05 | 江苏大学 | Health food or Chinese herbal compound for improving male sexual function and ultrasonic preparation method |
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