CN106942703A - Zeins chitosan complex coacervation thing and multi-mode supersonically preparation method - Google Patents
Zeins chitosan complex coacervation thing and multi-mode supersonically preparation method Download PDFInfo
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Abstract
The invention discloses zeins chitosan complex coacervation thing and multi-mode supersonically preparation method, belong to food embedding techniques field.It is to be prepared from by the raw material of following parts by weight：Zeins：1~10 part；Chitosan：1~2.5 part.The present invention adds chitosan in zeins, change the higher structure of zeins, open its structure, exposure active group, protein and polysaccharide form small aggregation simultaneously, so as to promote each aggregation to provide basis by hydrophobic interaction formation nano particle for embedding bioactive ingredients.The present invention is in the preparation process of the zeins chitosan complex coacervation thing of embedding resveratrol simultaneously, use multi-mode ultrasonication technology, protein is promoted by the method for physics and polysaccharide is cross-linked into aggregation, so as to promote each aggregation to provide basis by hydrophobic interaction formation nano particle for embedding bioactive ingredients.
Description
Technical field
The present invention relates to food embedding techniques field, refer in particular to a kind of using zeins and chitosan as raw material, use
The ultrasonically treated method for preparing complex coacervation thing and being embedded to resveratrol of multi-mode.
Background technology
The bioactive ingredients such as vitamin, unrighted acid, polyphenol compound or probiotics, with regulation human body life
The function of function is managed, the generation with preventing chronic disease can be delayed.However, above-mentioned active component is to food processing and stores
The factor such as temperature, oxygen, light, pH and metal ion is sensitive in journey, it is prone to aoxidize, isomerization, aggregation or the structure such as degrade change
Become, cause bioactivity to reduce or lose.Moreover, hydrophobicity and amphipathic active component solubility in water are very low.These
It greatly limit application of the bioactive ingredients in food and medicine industry.Bioactive ingredients are embedded
It is a kind of effective means for protecting, digesting and increasing bioavilability in food processing and storage to it.Natural life
Thing macromolecular (such as protein, polysaccharide) not only has high nutritive value, and with a variety of functional characteristics, is used extensively
Make the raw material in embedding techniques.
Zeins (zein) is once called as zein, and Japanese scholars are referred to as " prolamin ", as the term suggests belong to
Alcohol soluble protein class, it is named as zein by John Gorham within 1821.It is mixed by the polypeptide of many different peptide chains,
Molecular size, dissolubility and the electric charge of these polypeptides are different from, and McKinney proposed zeins first in 1958
(zein) two kinds of compositions of α-zein and β-zein are mainly included, alpha-alcohol soluble protein (α-zein) refers to the egg for being dissolved in 95% ethanol
In vain, the 80% of all alcohol soluble proteins is accounted for.The molten albumen of β -ol (β-zein) is dissolved in 60% ethanol and the ethanol insoluble in 95%, should
Albumen is comparatively unstable, easily occurs to be not present in retrogradation or gelling, industrially prepared zeins.With the molten egg of β -ol
In vain comparatively, alpha-alcohol soluble protein more lacks histidine, arginine, proline and methionine.Pomes proposes β -ol molten albumen point
Larger son amount is probably to be made up of alpha-alcohol soluble protein disulfide bond, because after it is hydrolyzed, gel electrophoresis occurs in that three
Main band, molecular weight is respectively：24000、22000、14000Da.
The unique structural property of zeins and amino acid composition determine that it is of low nutritive value, is difficult by organism
The characteristics of utilization, it is difficult to be used directly as raw-food material, cause its direct applied limitation in food production field.
But zeins have it is cohesives, bright, hydrophobic, hinder oxygen and the effects such as easy film forming, institute's film forming has certain toughness, and
The characteristic such as smooth, water-fast, oil resistant, anti-corrosion, has well in excipient substance, packaging material for food, biodegradable plastic field etc.
Potential application foreground, with great commercial value.Research such as Beck shows that zeins can be used as medicinal tablet
Coating.It can not be digested under one's belt, so it with regard to can be used to protecting effective component in stomach it is intact, but can
Dissolved in enteron aisle and slowly discharge its coatings (Beck M.I, Tomka I, Waysek E.Physico-chemical
charac-terization of zein as a film coating polymer-A direct compari-son with
with ethyl cellulose[J].Pharm.1996,14:137-150.).Li Xiuming etc. is at zeins (zein)
In with the addition of and gathered polypropylene carbonate (PPC), Zein-PPC laminated films are prepared by extrusion, PPC addition increases
The strong tensile strength and water resistance of composite membrane, the film of preparation for a kind of good degradable biological material (Li Xiuming, it is old
Open country, Wang Junyu waits .PPC to influence [J] Food Sciences of extrusion forming Zein-PPC laminated film properties, 2012, (23):6-
10.).Seung Yong Cho et al. have carried out the composite film packaging olive oil examination that zeins and soybean protein are made
Test, result of study shows, composite membrane can significantly slow oxidation and the corruption of olive oil, reached good resistance oxidation (Cho
SY,Lee SY,Rhee C.Edible oxygen barrier bilayer film pouches from corn zein
and soy protein isolate for olive oil packaging[J].LWT-Food Science and
Technology.2010,43(8):1234-1239.)。
Chitosan (chitosan) is also known as chitosan, is chitin (chitin) warp being widely present by nature
Cross what deacetylation was obtained, chemical name is Chitosan (1-4) -2- amino-B-D glucose.It is only second in nature
The second largest natural polysaccharide of cellulose, abundance has the advantages that safety non-toxic, degradable, good biocompatibility.Cause
Free amine group is carried in chitosan molecule, in an acidic solution easily into salt, in cationic property.Chitosan with its molecule contain ammonia
Radix amount increases, and its amino characteristic is more notable, the place of this exactly its peculiar property, has thus established many lifes of chitosan
The basis of thing characteristic and processing characteristics.Thus, chitosan is not only a kind of natural resources, even more a kind of new material medicine,
There is good application prospect in the fields such as chemical industry, agricultural.
Resveratrol is a kind of biological very strong natural Polyphenols anthraquinone terpene compound, also known as resvertrol, is swollen
The chemopreventive agent of knurl, is also to reduction platelet aggregation, prevention and treatment atherosclerosis, the chemistry of cardiovascular and cerebrovascular disease
Prophylactic, with effects such as anti-oxidant, antitumor, protection livers.But its property is unstable, poorly water-soluble, bioavilability
It is low, easily aoxidized under strong acid and strong base, illumination and higher temperature, and easily decompose broken in gastric environment after being taken in through human body
Badly lose activity, therefore a kind of carrier of resveratrol of research and development, it is encapsulated, to keep its activity and improve
Its bioavailability is particularly necessary.
The interphase interaction of research discovery protein and polysaccharide, formation complex coacervation thing can overcome one-component solubility
Bioactive ingredients are played embedding and protective effect well by difference, the deficiency that stability is poor, embedding efficiency is low.Therefore it is domestic
The research that outer scholar has carried out the preparation method of substantial amounts of protein-polysaccharide complex coacervation thing and embedded to bioactive ingredients.
Emulsion process is the common method for preparing complex coacervation thing, and the deficiency of this method, which to be shown, needed in the preparation process of complex coacervation thing and add
Plus organic solvent, surfactant, glutaraldehyde cross-linking agent etc., the residual of these reagents makes having for complex coacervation thing certain malicious
Property；Solvent method and chemical crosslink technique is gone to be directed to the introducing of toxic cross-linking agents；Therefore these three methods are not to prepare complex coacervation
The Perfected process of thing.Ionic cross-linking and self-assembly method preparation complex coacervation thing, mild condition, it is not necessary to poisonous crosslinking agent,
Chemical combination key is not generated, non-covalent bond is only relied on and is attached, thus is widely used.Particularly self-assembly method, is passed for nutrition
Send for system design, security etc. is relatively more preferably.But protein and polysaccharide are two kinds of macromoleculars of different nature after all, only
Only it is condensed by simple self-assembly method, flocculating result is poor, condensation product is low to the embedding efficiency of bioactive ingredients.
In order to solve this problem, the present invention introduces advanced multi-mode ultrasonic technology, it is desirable to which ultrasonic wave energy excites egg
Two kinds of biological macromolecule solns of white matter and polysaccharide produce the resonant frequency matched with its own intrinsic frequency, produce crosslinking, obtain
Obtained that a kind of embedding efficiency is high, pH is stable, the protein-polysaccharide complex coacervation thing of uniform particle diameter, and utilized the protein-many of gained
Sugared complex coacervation thing is embedded and protected to bioactive ingredients.
The content of the invention
To solve the above problems, the invention mainly passes through zeins-chitosan complex coacervation, the white black false hellebore of addition
The technological means such as alcohol, multi-mode ultrasonication prepare zeins-chitosan complex coacervation thing, and study its dialogue lamb's-quarters
The embedding effect of reed alcohol.
Zeins of the present invention-chitosan complex coacervation thing, is prepared from by the raw material of following parts by weight：
Zeins：1~10 part；
Chitosan：1~2.5 part.
It is preferred that being prepared from by the raw material of following parts by weight：
Zeins：1 part；
Chitosan：2.5 parts.
Wherein described chitosan molecule amount is 100,000 dalton or 150,000-30 ten thousand dalton.
Wherein described zeins is commercially available prod.
Wherein described zeins is prepared as steps described below：
(1) cross 60 after maize yellow-powder is crushed using pulverizer to sieve, according to solid-liquid ratio 1:10 (mg/ml) add absolute ethyl alcohol；
(2) counter current ultrasonic wave aided bleaching 40min, then 4000r/min centrifuge 5min separate and collect supernatant；Filter cake is treated
Further ultrasonic extraction zeins；
(3) filter cake in step (2) is according to solid-liquid ratio 1:8 (mg/ml) add 80% ethanol, double frequency frequency sweep ultrasonic ripple auxiliary
Extract, then 4000r/min centrifuges 5min and separates and collects supernatant；
(4) above-mentioned supernatant pours into the sodium chloride solution that 2 times of volume mass concentration are 0.6%, reclaims zeins,
4 DEG C of standing 24h, then collect precipitation, wash repeatedly, after 40 DEG C of heated-air drying 4h, be ground into zeins finished product.
The design parameter of adverse current impulse ultrasound aided bleaching is 1~5s of pulse width, pulse spacing 1 wherein in step (2)
~5s, 50~80W/L of ultrasonic power density；Supersonic frequency 20kHz, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent.
Double frequency frequency sweep ultrasonic wherein described in step (3) fixes upper and lower plates spacing when working be 10cm, frequency sweep cycle
300s, intermittently than 1:1.2 (ultrasonic 10s, interval 12s), every piece of vibration plate power is 600W up and down.
Double frequency combination of frequency wherein described in step (3) is：33kHz/68kHz、33kHz/40kHz、33kHz/
28kHz；Most preferably good combination of frequency is 33kHz/68kHz.
Wherein described zeins-chitosan complex coacervation thing, is prepared as steps described below：
(1) zeins is dissolved into 70% ethanol, obtains zeins concentration for 0.06g/mL solution,
Magnetic agitation is to being completely dissolved；(4 DEG C) centrifugation 10min of low temperature under the conditions of 8000r/min；Take 0.75mL supernatants, vortex oscillation
Under the conditions of be distributed to 2mL deionized waters dropwise；
(2) resveratrol will be entered into above-mentioned mixed solution under the conditions of lucifuge so that zeins and white lamb's-quarters
The mass ratio of reed alcohol is 10：1.
(3) chitosan will be entered into above-mentioned mixed solution by a certain percentage so that zeins and chitosan
Mass ratio is：(1~10)：(1~2.5).
(4) multifrequency mode ultrasound processing is carried out simultaneously under the conditions of lucifuge, wherein described multiple frequency ultrasonic tupe is：
Three frequencies or double frequency or the processing of single-frequency synchronizing ultrasound, 80~120W/L of ultrasonic power density；Ultrasonic pulse working time 10s；
Interpulse period 3s, sonication treatment time is 40min.
Wherein three frequencies or double frequency or the ultrasonic frequency of single-frequency synchronizing ultrasound processing are combined as：20kHz、28kHz、
28/40kHz or 20/28/40kHz, preferably 20/28/40kHz.
(5) after ultrasound terminates, 6h is stood at room temperature, and the zeins-chitosan for producing embedding resveratrol is combined
Zeins-chitosan complex coacervation of resveratrol must be embedded after condensation product solution, spray drying or freeze-drying
Composition granule.
The beneficial effects of the present invention are：
(1) present invention adds chitosan in zeins, changes the higher structure of zeins, makes its structure
Open, exposure active group, while protein and polysaccharide form small aggregation, so that it is hydrophobic mutual to promote each aggregation to pass through
Effect forms nano particle and provides basis for embedding bioactive ingredients.
(2) present invention makes in the preparation process of zeins-chitosan complex coacervation thing of embedding resveratrol
With multi-mode ultrasonication technology, protein is promoted by the method for physics and polysaccharide is cross-linked into aggregation, so as to promote
Each aggregation provides basis by hydrophobic interaction formation nano particle for embedding bioactive ingredients.
(3) preparation method of zeins-chitosan complex coacervation thing of resveratrol, technique are embedded in the present invention
It is simple to operate, organic reagent, suitable industrialized production, and zeins and chitosan raw material valency are not directed in preparation process
Lattice are cheap, and preparation technology is simple.
(4) zeins-chitosan complex coacervation thing of embedding resveratrol of the invention has good stability, life
Thing compatibility is good, slow-release time length, contain the advantages of rate is high, can be applied to multiple necks such as food, health products, medicine and cosmetics
Domain.
(5) present invention is using maize yellow-powder as raw material, and separation and Extraction zeins therein is simultaneously white as embedding is prepared
The raw material of the zeins of veratryl alcohol-chitosan complex coacervation thing, is conducive to the comprehensive utilization of corn processing accessory substance, together
The added value of Shi Tigao maize yellow-powders.
Brief description of the drawings
Fig. 1 is the multi-mode ultrasonic wave biological processing equipment structure chart of the present invention, wherein 1,2,3 be ultrasonic vibrating plate, 4 be Sheng
Liquid device, 5 be water-bath, and 6 be temp probe, and 7 be circulating pump, and 8 be Computerized controller, and 9,10,11 be ultrasonic controller.
Fig. 2 is the equipment drawing of frequency sweep double-frequency ultrasound pretreatment unit, 1- ultrasounds pond, 2- thermometers, 3- thermostatted water bathing pools, 4-
Vibration plate under vibration plate on ultrasonic wave, 5- ultrasonic waves, 6- sample treatments region, 7- computer controllers, 8- supersonic generators.
Fig. 3 is adverse current ultrasound processing equipment structural representation, and 1 is energy-gathered ultrasonic probe, and 2 be ultrasonic wave controller, 3
It is charging aperture for ultrasonic cup type chamber, 4,5 be circulating pump, and 6 is contain liquid device, and 7 be discharging opening.
Embodiment
Used term, unless otherwise specified, typically has those of ordinary skill in the art usual in the present invention
The implication of understanding.The present invention is described in further detail with reference to specific embodiment, and with reference to data.It should be understood that these
Embodiment is of the invention solely for the purpose of illustration, rather than limits the scope of the present invention in any way.
Fig. 1 is multi-mode ultrasonic wave biological processing equipment of the invention, and the equipment is furnished with a Computerized controller 8,
Ultrasound works parameter (ultrasonic power density, frequency, pulse working time, intermittent time and processing total time) can be set to control respectively
Three ultrasonic controllers 9,10,11 are made, the ultrasonic vibrating plate 1,2,3 of three different frequencies is connected respectively, single-frequency/two can be achieved
Individual frequency/tri- frequency ultrasonic wave processing；Solution input to be processed will be needed to contain single-frequency/double frequency/multiple frequency ultrasonic is carried out in liquid device 4
Assistance enzymolysis is tested, and starts circulating pump 7 and solution is circulated.Oneself of solution temperature is realized by water-bath 5 and temp probe 6
Dynamic control.
The equipment drawing for the frequency sweep double-frequency ultrasound pretreatment unit that Fig. 2 uses for the present invention, is Jiangsu University's independent development.It is super
Acoustic generator 8 can send the ultrasonic wave for determining frequency and frequency sweep both of which, and separate unit supersonic generator power is 600w.In ultrasonic pond
Placement ultrasonic wave vibration plate upper plate 4 symmetrical above and below and ultrasonic wave lower plate 5, are controlled on ultrasonic wave vibration plate by supersonic generator 8 in 1
Plate 4 and ultrasonic wave lower plate 5；After the setting each parameter of ultrasonic wave of computer controller 7, supersonic generator 8 is controlled, sends and meets the requirements
Ultrasonic wave；3 be the thermostatted water bathing pool of equipment in the present invention, passes through the monitoring temperature, and being adjusted according to need of work in real time of thermometer 2
Save medium temperature；Need to liquid raw material to be processed be placed in sample sack to be placed in treatment fluid region 6 and carry out ultrasonication.
Fig. 3 is the adverse current ultrasound processing equipment of the present invention, and the equipment is furnished with ultrasonic wave controller 2, controls ultrasound parameter, poly-
The material that energy formula ultrasonic probe 1 enters ultrasonic cup type chamber 3 to charging aperture 4 is handled, and starts 5 pairs of circulating pump while ultrasonic
Feed liquid is circulated, and 6 is contain liquid device, and 7 be discharging opening.
Chitosan in the present invention is commercially available prod, and molecular weight is 100,000 dalton or 150,000-30 ten thousand dalton.
Zeins in the present invention is commercially available prod.
Zeins in the present invention is prepared as steps described below：
(1) cross 60 after maize yellow-powder is crushed using pulverizer to sieve, according to solid-liquid ratio 1:10 (mg/ml) add absolute ethyl alcohol；
(2) counter current ultrasonic wave decolouring 40min, then 4000r/min centrifuge 5min separate and collect supernatant；Filter cake is treated into one
Walk ultrasonic extraction zeins；Design parameter is pulse width 5s, pulse spacing 5s, ultrasonic power density 80W/L；Ultrasound
Frequency 20kHz, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent.
(3) filter cake in step (2) is according to solid-liquid ratio 1:8 (mg/ml) add 80% ethanol, double frequency frequency sweep ultrasonic ripple auxiliary
Extract, then 4000r/min centrifuges 5min and separates and collects supernatant；Upper and lower plates spacing is fixed when double frequency frequency sweep ultrasonic works is
10cm, frequency sweep cycle 300s, intermittently than 1:1.2 (ultrasonic 10s, interval 12s), every piece of vibration plate power is 600W up and down；Double frequency is frequently
Rate is combined as：33kHz/68kHz.
(4) above-mentioned supernatant pours into the sodium chloride solution that 2 times of volume mass concentration are 0.6%, reclaims zeins,
4 DEG C of standing 24h, then collect precipitation, wash repeatedly, after 40 DEG C of heated-air drying 4h, be ground into zeins finished product, measure
Wherein protein content is 90.5%.
Embodiment 1-6 (is not added with ultrasound)
The ratio of zeins and chitosan is shown in Table 1, and chitosan molecule amount is 100,000 dalton.
Zeins-chitosan complex coacervation thing, is prepared as steps described below：
(1) zeins is dissolved into 70% ethanol, obtains zeins concentration for 0.06g/mL solution,
Magnetic agitation is to being completely dissolved；(4 DEG C) centrifugation 10min of low temperature under the conditions of 8000r/min；Take 0.75mL supernatants, vortex oscillation
Under the conditions of be distributed to 2mL deionized waters dropwise；
(2) resveratrol will be entered into above-mentioned mixed solution under the conditions of lucifuge so that zeins and white lamb's-quarters
The mass ratio of reed alcohol is 10：1.
(3) chitosan will be entered into above-mentioned mixed solution by a certain percentage, the quality of zeins and chitosan
Ratio is shown in Table 1.
(4) 6h is stood at room temperature, produces zeins-chitosan complex coacervation thing solution of embedding resveratrol,
Resveratrol envelop rate is determined as steps described below, and the Resveratrol content that dissociates is determined:Take the corn alcohol of cladding resveratrol molten
Albumen-chitosan complex coacervation thing solution, centrifuges 20min under 8 000 × g through super filter tube (molecular cut off 10kD), takes filter
The μ L of liquid 500 constant volumes in 25mL volumetric flasks, determine the light absorption value at A306, and calculate free resveratrol quality.According to following formula
Calculate the envelop rate and load factor of resveratrol：
The envelop rate (%) of the resveratrol=white black false hellebore in (resveratrol gross mass-free resveratrol quality) × 100/
Alcohol gross mass；
The load factor (%) of resveratrol=(resveratrol gross mass-free resveratrol quality) × 100/ sample is total
Quality.
Embodiment 2-6 preparation process be the same as Example 1, simply zeins/chitosan mass is different, is specifically shown in Table 1
Different zeins/chitosan mass compares the influence of resveratrol envelop rate and load factor.
Different zeins/the chitosan masses of table 1 compare the influence of resveratrol envelop rate and load factor
By comparative example 1-6 differences zeins/chitosan mass in table 1 compare resveratrol envelop rate and
The influence of load factor can be seen that (is not added with chitosan, i.e. embodiment 6) compared with the control, it can be found that addition chitosan can be with
The envelop rate that zeins embeds resveratrol is significantly improved, zeins/chitosan mass ratio is 1:1
When can make resveratrol envelop rate improve 36.2%, and zeins/chitosan mass ratio be 1:2.5
Shi Ze makes the envelop rate of resveratrol improve 42.0%.
Embodiment 7-10 (plus ultrasound)
Zeins：1 part；
Chitosan：1 part, chitosan molecule amount is 100,000 dalton.
Zeins-chitosan complex coacervation thing, is prepared as steps described below：
(1) zeins is dissolved into 70% ethanol, obtains zeins concentration for 0.06g/mL solution,
Magnetic agitation is to being completely dissolved；(4 DEG C) centrifugation 10min of low temperature under the conditions of 8000r/min；Take 0.75mL supernatants, vortex oscillation
Under the conditions of be distributed to 2mL deionized waters dropwise；
(2) resveratrol will be entered into above-mentioned mixed solution under the conditions of lucifuge so that zeins and white lamb's-quarters
The mass ratio of reed alcohol is 10：1.
(3) chitosan will be entered into above-mentioned mixed solution by a certain percentage, the quality of zeins and chitosan
Ratio is 1：1.
(4) multifrequency mode ultrasound processing is carried out simultaneously under the conditions of lucifuge, wherein described multiple frequency ultrasonic tupe is：
Three frequencies or double frequency or the processing of single-frequency synchronizing ultrasound, ultrasonic power density 120W/L；Ultrasonic pulse working time 10s；Pulse
Intermittent time 3s.Wherein ultrasonically treated ultrasonic frequency is 28kHz.
(5) after ultrasound terminates, 6h is stood at room temperature, and the zeins-chitosan for producing embedding resveratrol is combined
Condensation product solution, determines resveratrol envelop rate as steps described below, and the Resveratrol content that dissociates is determined:Take cladding resveratrol
Zeins-chitosan complex coacervation thing solution, centrifuged through super filter tube (molecular cut off 10kD) under 8 000 × g
20min, takes the μ L of filtrate 500 constant volumes in 25mL volumetric flasks, determines the light absorption value at A306, and calculate free resveratrol matter
Amount.The envelop rate and load factor of resveratrol are calculated according to following formula：
The envelop rate (%) of the resveratrol=white black false hellebore in (resveratrol gross mass-free resveratrol quality) × 100/
Alcohol gross mass；
The load factor (%) of resveratrol=(resveratrol gross mass-free resveratrol quality) × 100/ sample is total
Quality.
(6) zeins-chitosan complex coacervation thing solution for embedding resveratrol is spray-dried or freezed
Zeins-chitosan complex coacervation composition granule of resveratrol must be embedded after drying.
Embodiment 8-10 preparation process be the same as Example 7, simply ultrasound parameter is slightly changed, the different ultrasound modes pair of table 2
The influence of resveratrol envelop rate and load factor.
By influence of the comparative example 7-10 differences ultrasound mode to resveratrol envelop rate and load factor in table 2,
It can be found that ultrasonication can significantly improve the encapsulating that zeins-chitosan complex coacervation thing embeds resveratrol
Rate, compared with the control (not ultrasonic), 28kHz single-frequency ultrasonication can make the envelop rate raising 25% of resveratrol, and
20/28/40kHz multi-frequency ultrasonic processing then makes the envelop rate of resveratrol improve 38%.
Influence of the different ultrasound modes of table 2 to resveratrol envelop rate and load factor
Claims (9)
1. zeins-chitosan complex coacervation thing, it is characterised in that be prepared from by the raw material of following parts by weight：
Zeins：1~10 part；
Chitosan：1~2.5 part.
2. zeins according to claim 1-chitosan complex coacervation thing, it is characterised in that by following parts by weight
Raw material be prepared from：
Zeins：1 part；
Chitosan：2.5 parts.
3. zeins according to claim 1 or 2-chitosan complex coacervation thing, it is characterised in that wherein described
Chitosan molecule amount be 100,000 dalton or 150,000-30 ten thousand dalton.
4. zeins according to claim 1 or 2-chitosan complex coacervation thing, it is characterised in that wherein described
Zeins prepared as steps described below：
（1）Maize yellow-powder is crossed 60 after being crushed using pulverizer and sieved, according to solid-liquid ratio 1:10（mg/ml）Add absolute ethyl alcohol；
（2）Counter current ultrasonic wave aided bleaching 40min, then 4000r/min centrifuge 5min separate and collect supernatant；Filter cake is treated into one
Walk ultrasonic extraction zeins；
（3）Step（2）In filter cake according to solid-liquid ratio 1:8（mg/ml）Add 80% ethanol, double frequency frequency sweep ultrasonic ripple assisted extraction,
Then 4000r/min centrifuges 5min and separates and collects supernatant；
（4）Above-mentioned supernatant pours into the sodium chloride solution that 2 times of volume mass concentration are 0.6%, reclaims zeins, 4 DEG C quiet
24h is put, precipitation is then collected, washes repeatedly, after 40 DEG C of heated-air drying 4h, be ground into zeins finished product.
5. zeins according to claim 3-chitosan complex coacervation thing, it is characterised in that wherein step（2）
The design parameter of middle adverse current impulse ultrasound aided bleaching is 1 ~ 5s of pulse width, 1 ~ 5s of pulse spacing, ultrasonic power density 50 ~ 80
W/L；The kHz of supersonic frequency 20, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent.
6. zeins according to claim 3-chitosan complex coacervation thing, it is characterised in that wherein step（3）
Described in the work of double frequency frequency sweep ultrasonic when to fix upper and lower plates spacing be 10cm, frequency sweep cycle 300s, intermittently than 1:1.2（Ultrasound
10s, interval 12s）, every piece of vibration plate power is 600W up and down.
7. zeins according to claim 3-chitosan complex coacervation thing, it is characterised in that wherein step（3）
Described in double frequency combination of frequency be： 33 kHz/ 68kHz、33kHz/40kHz、33 kHz/28 kHz；Most preferably good frequency
It is combined as 33 kHz/68 kHz.
8. the preparation method of zeins according to claim 1 or 2-chitosan complex coacervation thing, its feature exists
In carrying out as steps described below：
（1）Zeins is dissolved into 70% ethanol, zeins concentration is obtained for 0.06g/mL solution, magnetic force
Stirring is to being completely dissolved；Low temperature under the conditions of 8000 r/min（4℃）Centrifuge 10min；Take 0.75 mL supernatants, vortex oscillation bar
2 mL deionized waters are distributed under part dropwise；
（2）Resveratrol will be entered into above-mentioned mixed solution under the conditions of lucifuge so that zeins and resveratrol
Mass ratio be 10：1；
（3）Chitosan will be entered into above-mentioned mixed solution by a certain percentage so that the quality of zeins and chitosan
Ratio is：（1~10）：（1~2.5）；
（4）Multifrequency mode ultrasound processing is carried out simultaneously under the conditions of lucifuge, wherein described multiple frequency ultrasonic tupe is：Three frequencies
Or double frequency or the processing of single-frequency synchronizing ultrasound, the W/L of ultrasonic power density 80 ~ 120；The s of ultrasonic pulse working time 10；Pulse
The s of intermittent time 3, sonication treatment time is 40min；
（5）After ultrasound terminates, 6h is stood at room temperature, produces zeins-chitosan complex coacervation of embedding resveratrol
Zeins-chitosan complex coacervation thing of resveratrol must be embedded after thing solution, spray drying or freeze-drying
Grain.
9. the preparation method of zeins according to claim 8-chitosan complex coacervation thing, it is characterised in that its
In three frequency or double frequency or single-frequency synchronizing ultrasound processing ultrasonic frequency be combined as：20kHz, 28 kHz, 28/40 kHz or
The kHz of person 20/28/40, preferably 20/28/40 kHz.
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| CN108034061A (en) * | 2017-12-01 | 2018-05-15 | 蒋春霞 | A kind of preparation method of bio-compatible type chitosan film |
| CN108606993A (en) * | 2018-05-25 | 2018-10-02 | 姜香 | A kind of preparation method of anti-migration type albumen transport agent material |
| CN109105616A (en) * | 2018-08-29 | 2019-01-01 | 江苏大学 | A kind of method that scanning frequency pulse supersonic synergic alkali prepares walnut protein |
| CN109438582A (en) * | 2018-10-16 | 2019-03-08 | 宝应县有机食品质量监督检验中心 | The method of multi-mode ultrasonic wave assisted extraction arrowhead starch |
| CN110754651A (en) * | 2019-10-30 | 2020-02-07 | 武汉轻工大学 | Preparation method of starch gel food additive |
| CN111150050A (en) * | 2020-01-11 | 2020-05-15 | 浙江工商大学 | Preparation method of resveratrol microcapsule |
| CN112205628A (en) * | 2020-09-18 | 2021-01-12 | 广州大学 | Composite condensate with double embedding functions and preparation method and application thereof |
| CN112402291A (en) * | 2020-11-26 | 2021-02-26 | 上海应用技术大学 | Composite wall material particle coated with fat-soluble sunscreen agent, preparation method and application |
| CN114098076A (en) * | 2021-11-18 | 2022-03-01 | 江南大学 | Preparation method of genipin-crosslinked quercetin-zein/pectin/chitosan nanoparticles |
| CN114557440A (en) * | 2022-02-15 | 2022-05-31 | 江南大学 | Stable zein-glycyrrhizic acid-chitosan Pickering emulsion gel and preparation method thereof |
| CN115251262A (en) * | 2022-08-11 | 2022-11-01 | 齐鲁工业大学 | Spray drying method for combined viscosity reduction of mulberry protein extract and chitosan |
| CN115530357A (en) * | 2022-09-28 | 2022-12-30 | 江南大学 | Genipin cross-linked alcohol-soluble protein carboxymethyl cellulose co-embedded functional factor hydrogel and preparation method thereof |
| CN115568545A (en) * | 2021-06-21 | 2023-01-06 | 扬州大学 | Chitosan-modified egg white protein-nisin nanoparticle as well as preparation method and antibacterial application thereof |
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| CN108034061A (en) * | 2017-12-01 | 2018-05-15 | 蒋春霞 | A kind of preparation method of bio-compatible type chitosan film |
| CN108606993A (en) * | 2018-05-25 | 2018-10-02 | 姜香 | A kind of preparation method of anti-migration type albumen transport agent material |
| CN109105616A (en) * | 2018-08-29 | 2019-01-01 | 江苏大学 | A kind of method that scanning frequency pulse supersonic synergic alkali prepares walnut protein |
| CN109438582A (en) * | 2018-10-16 | 2019-03-08 | 宝应县有机食品质量监督检验中心 | The method of multi-mode ultrasonic wave assisted extraction arrowhead starch |
| CN110754651A (en) * | 2019-10-30 | 2020-02-07 | 武汉轻工大学 | Preparation method of starch gel food additive |
| CN111150050A (en) * | 2020-01-11 | 2020-05-15 | 浙江工商大学 | Preparation method of resveratrol microcapsule |
| CN111150050B (en) * | 2020-01-11 | 2022-09-30 | 浙江工商大学 | Preparation method of resveratrol microcapsule |
| CN112205628A (en) * | 2020-09-18 | 2021-01-12 | 广州大学 | Composite condensate with double embedding functions and preparation method and application thereof |
| CN112402291A (en) * | 2020-11-26 | 2021-02-26 | 上海应用技术大学 | Composite wall material particle coated with fat-soluble sunscreen agent, preparation method and application |
| CN115568545A (en) * | 2021-06-21 | 2023-01-06 | 扬州大学 | Chitosan-modified egg white protein-nisin nanoparticle as well as preparation method and antibacterial application thereof |
| CN115568545B (en) * | 2021-06-21 | 2024-05-17 | 扬州大学 | Chitosan modified egg white protein-nisin nanoparticle as well as preparation method and antibacterial application thereof |
| CN114098076A (en) * | 2021-11-18 | 2022-03-01 | 江南大学 | Preparation method of genipin-crosslinked quercetin-zein/pectin/chitosan nanoparticles |
| CN114557440A (en) * | 2022-02-15 | 2022-05-31 | 江南大学 | Stable zein-glycyrrhizic acid-chitosan Pickering emulsion gel and preparation method thereof |
| CN115251262A (en) * | 2022-08-11 | 2022-11-01 | 齐鲁工业大学 | Spray drying method for combined viscosity reduction of mulberry protein extract and chitosan |
| CN115251262B (en) * | 2022-08-11 | 2023-11-17 | 齐鲁工业大学 | Spray drying method for reducing viscosity by combining mulberry protein extract and chitosan |
| CN115530357A (en) * | 2022-09-28 | 2022-12-30 | 江南大学 | Genipin cross-linked alcohol-soluble protein carboxymethyl cellulose co-embedded functional factor hydrogel and preparation method thereof |
| CN115530357B (en) * | 2022-09-28 | 2024-02-27 | 江南大学 | Genipin crosslinked prolamin carboxymethyl cellulose co-embedded functional factor hydrogel and preparation method thereof |
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