CN108034061A - A kind of preparation method of bio-compatible type chitosan film - Google Patents
A kind of preparation method of bio-compatible type chitosan film Download PDFInfo
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- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/06—Pectin; Derivatives thereof
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- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
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Abstract
The invention discloses a kind of preparation method of bio-compatible type chitosan film, belong to biomaterial preparing technical field.Curcumin and zeins ethanol solution are mixed and stirred for rear curcumin in chitosan film special interaction occurs with zeins, micro-current is produced between negative oxygen ion in carbon nano tube surface and curcumin phenyl ring after phenolic hydroxyl group dehydrogenation, be conducive to the transmission information of bioelectricity, lift the biological conductive performance of chitosan film；Beet pectin has a variety of physiological activity in the present invention, can be by stimulating intestines peristalsis to remove noxious material in enteron aisle, curcumin is wrapped in inside zeins particle, since the dissolubility of curcumin in water is minimum, it can be separated out from water phase, make chitosan slow mechanism dissolved in vivo, play slow releasing function, curcumin can reduce surrounding lipids contents and have antioxidation, so as to strengthen the antioxygenic property of chitosan film, have a extensive future in medical science field.
Description
Technical field
The invention discloses a kind of preparation method of bio-compatible type chitosan film, belongs to biomaterial technology of preparing neck
Domain.
Background technology
Chitosan is also known as chitosan, is that the chitin being widely present by nature is obtained by deacetylation
, from 1859, after Frenchman obtains chitosan first, the biological functionality and compatibility of this natural polymer, blood phase
The excellent performances such as capacitive, security, microbic resolvability by all trades and professions extensive concern, medicine, food, chemical industry, cosmetics,
The application study of the numerous areas such as water process, METAL EXTRACTION and recycling, biochemistry and biomedical engineering achieves major progress.Pin
To patient, chitosan reducing blood lipid, hypoglycemic effect have research report.Meanwhile chitosan is by as thickener, fruit glaze agent row
Enter state food additive and use standard GB-2760.Excellent film forming causes it to be increasingly valued by people.Medicine is led
Domain is one of important application field of chitosan film, and it is more that it is mainly used for suture, Wound dressing and organizational project
In the stent of hole.
The method that tradition prepares chitosan film has three kinds of wet spinning process, dry spinning method and method of electrostatic spinning.Dry method
Spinning and wet spinning difference lies in：Stoste thread will first pass through one section of air layer before coagulating bath is entered, and this section of sky
Gas-bearing formation plays a crucial role the structure and performance of film.Method of electrostatic spinning is added in polymer solution or melt
The high-pressure electrostatic of upper kilovolt or higher, makes powered polymer drop be acted on capillary tip by electric field force and is accelerated to be formed
One taylor cone, when electric field force is sufficiently large, and polymer drop can be made to overcome surface tension, polymer drop will form injection
Thread, jet stream solvent is evaporated in course of injection, is fallen curing molding on the reception device, is formd chitosan film.
Current biological electrochemistry is more and more big to the demand applied to the conductive material inside organism, and passes through Wet-spinning
Chitosan film low cross-intensity and electric conductivity prepared by silk method, dry spinning method and method of electrostatic spinning is poor, it is difficult to
Such field is applied.The prior art production chitosan film also there are antioxygenic property it is poor the shortcomings that.
Therefore, a kind of conduct electricity very well and chitosan film that antioxygenic property is good is to chitosan film technology of preparing is invented
Field has positive effect.
The content of the invention
Present invention mainly solves technical problem, for chitosan film be applied to organism inside when electric conductivity it is poor,
A kind of the defects of antioxygenic property is poor, there is provided preparation method of bio-compatible type chitosan film.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of preparation method of bio-compatible type chitosan film, it is characterised in that specifically preparation process is：
（1）30~35g chitosans are taken to be placed in 100~120mL acetic acid solutions, heat temperature raising, starts blender, is stirred,
Until after chitosan is completely dissolved, continuously adds 50~80mL distilled water and obtain chitosan film liquid；（2）Take 70~80g corn eggs
White powder is put into mortar and grinds, and sieving obtains corn protein powder, and corn protein powder is put into 200~230mL petroleum ethers and is soaked,
Filter and be put into after removing filtrate in vacuum drying chamber, after dry, obtain decoloration corn protein powder；
（3）Decoloration corn protein powder is mixed with ethanol solution, heat temperature raising obtains corn protein powder suspension, by corn egg
White powder suspension, which is placed in ultrasonic machine, to be ultrasonically treated, and obtains sonification fluid；
（4）Sonification fluid is placed in centrifugal treating in centrifugal separator, lower sediment thing is removed, obtains supernatant liquor i.e. corn
The ethanol solution of alcohol soluble protein, 30~35g beet pectins is added in the beaker equipped with 150~160mL deionized waters, to beaker
Middle addition 140~160mL chitosan film liquids, are mixed with magnetic stirrer, obtain composite glue solution；
（5）10~15g curcumins, 6~8g carbon nanometers are added into the ethanol solution of above-mentioned 200~220mL zeins
Pipe, obtains mixed liquor, composite glue solution is mixed with mixed liquor, is put into Rotary Evaporators, rotary evaporation, after still aging, is obtained
To complex sol；
（6）Complex sol is coated on one piece of poly (methyl methacrylate) plate, it is organic with another piece of poly (methyl methacrylate) plate and coating sol liquid
After glass plate fitting compresses, it is transferred in baking oven, after dry, takes out double-deck poly (methyl methacrylate) plate, after cooled to room temperature, by two
Block organic glass is separated along edge, and surface film of tearing, obtains chitosan film.
Step（1）The deacetylation of the chitosan is 90~95%, and the mass fraction of acetic acid solution is 5%, and heating rises
Temperature is 60~70 DEG C after temperature, and speed of agitator is 300~350r/min.
Step（2）It is 30~40min that the corn protein powder, which is put into milling time in mortar, and specification of sieving is 200
Mesh, soaking time are 2~3h, and the design temperature of vacuum drying chamber is 70~75 DEG C, and drying time is 4~5h.
Step（3）The mass fraction of the ethanol solution is 80%, the matter that decoloration corn protein powder is mixed with ethanol solution
Measure ratio be 1 ︰ 10, after heat temperature raising temperature be 50~55 DEG C, sonication treatment time is 20~25min, control ultrasound unit frequency be
22~25kHz.
Step（4）The centrifugal separator rotating speed is 7000~8000r/min, and the centrifugal treating time is 15~20min,
Magnetic stirring apparatus rotating speed is 700~800r/min, and it is 30~35min to be stirred the time.
Step（5）The volume ratio that the composite glue solution is mixed with mixed liquor is 1 ︰ 2, the rotating speeds of Rotary Evaporators for 80~
90r/min, rotary evaporation time are 20~30min, and the still aging time is 12~14h.
Step（6）The complex sol controls coating thickness when being coated be 0.8~1.2mm, and baking oven design temperature is 50
~55 DEG C, drying time is 4~5h.
The beneficial effects of the invention are as follows：
（1）Chitosan is put into acetic acid solution by the present invention, and heating stirring dissolves to obtain chitosan film liquid, and corn protein powder is ground
Decolorization in petroleum ether is soaked in after mill, sieving, then through filtering, being dried to obtain decoloration corn protein powder, by the corn egg that decolourizes
White powder is mixed with ethanol solution by certain mass ratio, and heating obtains corn protein powder suspension, by supersound process, high speed centrifugation
Obtain the ethanol solution of zeins, by beet pectin add deionized water in, then mix chitosan film liquid obtain it is compound
Glue, after the ethanol solution of composite glue solution, zeins is mixed incorporation curcumin, carbon nanotubes, process is still aging
Complex sol is obtained, after complex sol is coated on poly (methyl methacrylate) plate, is bonded therewith with another piece of poly (methyl methacrylate) plate, after dry
Film of tearing obtains bio-compatible type chitosan film, mixes curcumin and zeins ethanol solution in chitosan film
After merging stirring, with zeins special interaction occurs for curcumin, and part curcumin molecule enters the molten egg of corn alcohol
In white hydrophobic cavities, curcumin is a kind of plant polyphenol, and the electron transport rate on phenyl ring is very fast, and carbon nanotubes is in organism
Sour environment in make carbon nano tube surface positively charged, carbon nano tube surface is born with the oxygen after phenolic hydroxyl group dehydrogenation on curcumin phenyl ring
Micro-current is produced between ion, is conducive to the transmission information of bioelectricity, lifts the biological conductive performance of chitosan film；
（2）Beet pectin has a variety of physiological activity in the present invention, can be by stimulating intestines peristalsis to remove Toxic in enteron aisle
Matter, due to not having the digestive ferment of polysaccharide in stomach and intestine, thus pectin is not easy to be digested in stomach and intestine, in the glucosides of colonic microflora secretion
Be decomposed under enzyme effect, active material discharged in colon, garden beet pectin as protective layer, can realize nutriment,
The conveying of bioactive ingredients, the curcumin in zeins, occurs in self assembling process, curcumin is wrapped in jade
Inside rice alcohol soluble protein particle, since the dissolubility of curcumin in water is minimum, curcumin enters supersaturation in water phase, can be from water
Separated out in phase, be deposited on zeins particle surface, the zeins particle surface of surface deposition curcumin is more
It is hydrophobic, make chitosan slow mechanism dissolved in vivo, play slow releasing function, curcumin can reduce surrounding lipids contents and have
Antioxidation, makes in chitosan film the readily oxidizable substance such as carbon nanotubes not be decomposed oxidation, so as to strengthen chitosan film
Antioxygenic property, have a extensive future in medical science field.
Embodiment
It is molten that the chitosan for taking 30~35g deacetylations to be 90~95% is placed in the acetic acid that 100~120mL mass fractions are 5%
In liquid, 60~70 DEG C are heated to, starts blender, is stirred with the rotating speed of 300~350r/min, until chitosan is complete
After fully dissolved, continuously add 50~80mL distilled water and obtain chitosan film liquid；Take 70~80g corn protein powders to be put into mortar to grind
Grind 30~40min, sieving obtains 200 mesh corn protein powders, by corn protein powder be put into 200~230mL petroleum ethers immersion 2~
3h, filters and is put into after removing filtrate in the vacuum drying chamber that design temperature is 70~75 DEG C, after dry 4~5h, it is beautiful to obtain decoloration
Rice gluten powder；With mass fraction it is that 80% ethanol solution is that 1 ︰ 10 is mixed in mass ratio by decoloration corn protein powder, is heated to
50~55 DEG C, obtain corn protein powder suspension, by corn protein powder suspension be placed in ultrasonic machine be ultrasonically treated 20~
25min, it is 22~25kHz to control ultrasonic unit frequency, obtains sonification fluid；Sonification fluid is placed in centrifugal separator,
With rotating speed 15~20min of centrifugal treating of 7000~8000r/min, lower sediment thing is removed, obtains supernatant liquor i.e. corn alcohol
The ethanol solution of molten albumen, 30~35g beet pectins is added in the beaker equipped with 150~160mL deionized waters, into beaker
140~160mL chitosan film liquids are added, 30~35min is stirred with the rotating speed of 700~800r/min with magnetic stirring apparatus,
Obtain composite glue solution；10~15g curcumins, 6~8g are added into the ethanol solution of above-mentioned 200~220mL zeins
Carbon nanotubes, obtains mixed liquor, and composite glue solution is mixed for 1 ︰ 2 by volume with mixed liquor, is put into Rotary Evaporators, with 80
The rotating speed of~90r/min, after rotary evaporation 20~30min, still aging 12~14h, obtains complex sol；Complex sol is applied
For cloth on one piece of poly (methyl methacrylate) plate, it is 0.8~1.2mm to control coating thickness, with another piece of poly (methyl methacrylate) plate and coating sol liquid
Poly (methyl methacrylate) plate fitting compress after, it is in 50~55 DEG C of baking oven to be transferred to design temperature, after dry 4~5h, is taken out double-deck
Poly (methyl methacrylate) plate, after cooled to room temperature, two pieces of organic glasses is separated along edge, surface film of tearing, obtains shell and gather
Sugared film.
Example 1
The chitosan for taking 30g deacetylations to be 90% is placed in the acetic acid solution that 100mL mass fractions are 5%, is heated to 60
DEG C, start blender, be stirred with the rotating speed of 300r/min, until after chitosan is completely dissolved, continuously add 50mL distillations
Water obtains chitosan film liquid；Taking 70g corn protein powders to be put into mortar and grind 30min, sieving obtains 200 mesh corn protein powders,
Corn protein powder is put into 200mL petroleum ethers and soaks 2h, being put into the vacuum that design temperature is 70 DEG C after suction filtration removal filtrate does
In dry case, after dry 4h, decoloration corn protein powder is obtained；Decoloration corn protein powder is pressed with mass fraction for 80% ethanol solution
Mass ratio mixes for 1 ︰ 10, is heated to 50 DEG C, obtains corn protein powder suspension, corn protein powder suspension is placed in super
20min is ultrasonically treated in sound machine, it is 22kHz to control ultrasonic unit frequency, obtains sonification fluid；Sonification fluid is placed in centrifugation
In seperator, with the rotating speed centrifugal treating 15min of 7000r/min, lower sediment thing is removed, it is molten to obtain the i.e. corn alcohol of supernatant liquor
The ethanol solution of albumen, 30g beet pectins are added in the beaker equipped with 150mL deionized waters, 140mL shells are added into beaker
Glycan film liquid, is stirred 30min with the rotating speed of 700r/min with magnetic stirring apparatus, obtains composite glue solution；It is beautiful to above-mentioned 200mL
10g curcumins, 6g carbon nanotubes are added in the ethanol solution of rice alcohol soluble protein, mixed liquor is obtained, by composite glue solution and mixed liquor
Mix, be put into Rotary Evaporators, with the rotating speed of 80r/min, rotary evaporation 20min, still aging 12h for 1 ︰ 2 by volume
Afterwards, complex sol is obtained；Complex sol is coated on one piece of poly (methyl methacrylate) plate, it is 0.8mm to control coating thickness, with another piece
After poly (methyl methacrylate) plate is bonded compression with the poly (methyl methacrylate) plate of coating sol liquid, it is transferred in the baking oven that design temperature is 50 DEG C, does
After dry 4h, double-deck poly (methyl methacrylate) plate is taken out, after cooled to room temperature, two pieces of organic glasses are separated along edge, table of tearing
Face film, obtains chitosan film.
Example 2
The chitosan for taking 32g deacetylations to be 92% is placed in the acetic acid solution that 110mL mass fractions are 5%, is heated to 65
DEG C, start blender, be stirred with the rotating speed of 320r/min, until after chitosan is completely dissolved, continuously add 60mL distillations
Water obtains chitosan film liquid；Taking 75g corn protein powders to be put into mortar and grind 35min, sieving obtains 200 mesh corn protein powders,
Corn protein powder is put into 210mL petroleum ethers and soaks 2.5h, filters and is put into the vacuum that design temperature is 72 DEG C after removing filtrate
In drying box, after dry 4.5h, decoloration corn protein powder is obtained；Decoloration corn protein powder and mass fraction is molten for 80% ethanol
Liquid mixes for 1 ︰ 10 in mass ratio, is heated to 52 DEG C, obtains corn protein powder suspension, corn protein powder suspension is put
22min is ultrasonically treated in ultrasonic machine, it is 24kHz to control ultrasonic unit frequency, obtains sonification fluid；Sonification fluid is placed in
In centrifugal separator, with the rotating speed centrifugal treating 17min of 7500r/min, lower sediment thing is removed, obtains supernatant liquor i.e. corn
The ethanol solution of alcohol soluble protein, 32g beet pectins are added in the beaker equipped with 155mL deionized waters, are added into beaker
150mL chitosan film liquids, are stirred 32min with the rotating speed of 750r/min with magnetic stirring apparatus, obtain composite glue solution；To above-mentioned
12g curcumins, 7g carbon nanotubes are added in the ethanol solution of 210mL zeins, obtains mixed liquor, by composite glue solution with
Mixed liquor mixes for 1 ︰ 2 by volume, is put into Rotary Evaporators, and with the rotating speed of 85r/min, rotary evaporation 25min, stands old
After changing 13h, complex sol is obtained；Complex sol is coated on one piece of poly (methyl methacrylate) plate, it is 1.0mm to control coating thickness, is used
After another piece of poly (methyl methacrylate) plate is bonded compression with the poly (methyl methacrylate) plate of coating sol liquid, the baking oven that design temperature is 52 DEG C is transferred to
In, after dry 4.5h, take out double-deck poly (methyl methacrylate) plate, after cooled to room temperature, two pieces of organic glasses separated along edge,
Tear surface film, obtain chitosan film.
Example 3
The chitosan for taking 35g deacetylations to be 95% is placed in the acetic acid solution that 120mL mass fractions are 5%, is heated to 70
DEG C, start blender, be stirred with the rotating speed of 350r/min, until after chitosan is completely dissolved, continuously add 80mL distillations
Water obtains chitosan film liquid；Taking 80g corn protein powders to be put into mortar and grind 40min, sieving obtains 200 mesh corn protein powders,
Corn protein powder is put into 230mL petroleum ethers and soaks 3h, being put into the vacuum that design temperature is 75 DEG C after suction filtration removal filtrate does
In dry case, after dry 5h, decoloration corn protein powder is obtained；Decoloration corn protein powder is pressed with mass fraction for 80% ethanol solution
Mass ratio mixes for 1 ︰ 10, is heated to 55 DEG C, obtains corn protein powder suspension, corn protein powder suspension is placed in super
25min is ultrasonically treated in sound machine, it is 25kHz to control ultrasonic unit frequency, obtains sonification fluid；Sonification fluid is placed in centrifugation
In seperator, with the rotating speed centrifugal treating 20min of 8000r/min, lower sediment thing is removed, it is molten to obtain the i.e. corn alcohol of supernatant liquor
The ethanol solution of albumen, 35g beet pectins are added in the beaker equipped with 160mL deionized waters, 160mL shells are added into beaker
Glycan film liquid, is stirred 35min with the rotating speed of 800r/min with magnetic stirring apparatus, obtains composite glue solution；It is beautiful to above-mentioned 220mL
15g curcumins, 8g carbon nanotubes are added in the ethanol solution of rice alcohol soluble protein, mixed liquor is obtained, by composite glue solution and mixed liquor
Mix, be put into Rotary Evaporators, with the rotating speed of 90r/min, rotary evaporation 30min, still aging 14h for 1 ︰ 2 by volume
Afterwards, complex sol is obtained；Complex sol is coated on one piece of poly (methyl methacrylate) plate, it is 1.2mm to control coating thickness, with another piece
After poly (methyl methacrylate) plate is bonded compression with the poly (methyl methacrylate) plate of coating sol liquid, it is transferred in the baking oven that design temperature is 55 DEG C, does
After dry 5h, double-deck poly (methyl methacrylate) plate is taken out, after cooled to room temperature, two pieces of organic glasses are separated along edge, table of tearing
Face film, obtains chitosan film.
Comparative example
With the bio-compatible type chitosan film of Shanghai company production as a comparison case to bio-compatible type produced by the present invention
Bio-compatible type chitosan film in chitosan film and comparative example carries out performance detection, and testing result is as shown in table 1：
1st, test method：
The ionic conductivity that ionic conductivity surveys film is measured on electrochemical apparatus by double probe AC impedance spectrometries, is tested
Frequency range is 0.1~1.0Hz.
Antioxygenic property test method：Accelerated oxidation test is done using Schaal Oven Methods, by example 1~3 and comparative example
In chitosan film be respectively placed in the relatively good insulating box of air and store 4d, sampled every 24h, according to national regulation
The method measure peroxide value of GB/T5009.37-2003, it is as follows to measure result：
Table 1
| Test event | Example 1 | Example 2 | Example 3 | Comparative example |
| Ionic conductivity（S·cm-1） | 1.05×10-2 | 1.06×10-2 | 1.08×10-2 | 0.7×10-2 |
| 24 it is small when peroxide value（meq/kg） | 1.61 | 1.57 | 1.52 | 3.14 |
| 48 it is small when peroxide value（meq/kg） | 2.15 | 2.11 | 2.08 | 4.35 |
| 72 it is small when peroxide value（meq/kg） | 3.21 | 3.19 | 3.15 | 6.86 |
| 96 it is small when peroxide value（meq/kg） | 4.54 | 4.51 | 4.46 | 8.57 |
Bio-compatible shell glycan membrane ion electric conductivity produced by the present invention greatly enhances according to above-mentioned middle data, ion
Electrical conductivity is up to 1.08 × 10-2（S·cm-1）, bio-compatible type chitosan film of the invention is than the chitosan film in comparative example
Peroxide value is small, slows down oxidation rate, and oxidation stability is greatly improved, and antioxygenic property is greatly improved, and has
Wide application prospect.
Claims (7)
1. a kind of preparation method of bio-compatible type chitosan film, it is characterised in that specifically preparation process is：
（1）30~35g chitosans are taken to be placed in 100~120mL acetic acid solutions, heat temperature raising, starts blender, is stirred,
Until after chitosan is completely dissolved, continuously adds 50~80mL distilled water and obtain chitosan film liquid；（2）Take 70~80g corn eggs
White powder is put into mortar and grinds, and sieving obtains corn protein powder, and corn protein powder is put into 200~230mL petroleum ethers and is soaked,
Filter and be put into after removing filtrate in vacuum drying chamber, after dry, obtain decoloration corn protein powder；
（3）Decoloration corn protein powder is mixed with ethanol solution, heat temperature raising obtains corn protein powder suspension, by corn egg
White powder suspension, which is placed in ultrasonic machine, to be ultrasonically treated, and obtains sonification fluid；
（4）Sonification fluid is placed in centrifugal treating in centrifugal separator, lower sediment thing is removed, obtains supernatant liquor i.e. corn
The ethanol solution of alcohol soluble protein, 30~35g beet pectins is added in the beaker equipped with 150~160mL deionized waters, to beaker
Middle addition 140~160mL chitosan film liquids, are mixed with magnetic stirrer, obtain composite glue solution；
（5）10~15g curcumins, 6~8g carbon nanometers are added into the ethanol solution of above-mentioned 200~220mL zeins
Pipe, obtains mixed liquor, composite glue solution is mixed with mixed liquor, is put into Rotary Evaporators, rotary evaporation, after still aging, is obtained
To complex sol；
（6）Complex sol is coated on one piece of poly (methyl methacrylate) plate, it is organic with another piece of poly (methyl methacrylate) plate and coating sol liquid
After glass plate fitting compresses, it is transferred in baking oven, after dry, takes out double-deck poly (methyl methacrylate) plate, after cooled to room temperature, by two
Block organic glass is separated along edge, and surface film of tearing, obtains chitosan film.
A kind of 2. preparation method of bio-compatible type chitosan film according to claim 1, it is characterised in that：
Step（1）The deacetylation of the chitosan is 90~95%, and the mass fraction of acetic acid solution is 5%, after heat temperature raising
Temperature is 60~70 DEG C, and speed of agitator is 300~350r/min.
A kind of 3. preparation method of bio-compatible type chitosan film according to claim 1, it is characterised in that：
Step（2）It is 30~40min that the corn protein powder, which is put into milling time in mortar, and specification of sieving is 200 mesh, leaching
The bubble time is 2~3h, and the design temperature of vacuum drying chamber is 70~75 DEG C, and drying time is 4~5h.
A kind of 4. preparation method of bio-compatible type chitosan film according to claim 1, it is characterised in that：
Step（3）The mass fraction of the ethanol solution is 80%, the mass ratio that decoloration corn protein powder is mixed with ethanol solution
For 1 ︰ 10, temperature is 50~55 DEG C after heat temperature raising, and sonication treatment time be 20~25min, control ultrasound unit frequency be 22~
25kHz。
A kind of 5. preparation method of bio-compatible type chitosan film according to claim 1, it is characterised in that：
Step（4）The centrifugal separator rotating speed is 7000~8000r/min, and the centrifugal treating time is 15~20min, magnetic force
Agitator speed is 700~800r/min, and it is 30~35min to be stirred the time.
A kind of 6. preparation method of bio-compatible type chitosan film according to claim 1, it is characterised in that：
Step（5）The volume ratio that the composite glue solution is mixed with mixed liquor is 1 ︰ 2, and the rotating speed of Rotary Evaporators is 80~90r/
Min, rotary evaporation time are 20~30min, and the still aging time is 12~14h.
A kind of 7. preparation method of bio-compatible type chitosan film according to claim 1, it is characterised in that：
Step（6）The complex sol controls coating thickness when being coated be 0.8~1.2mm, and baking oven design temperature is 50~55
DEG C, drying time is 4~5h.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN109517226A (en) * | 2019-01-07 | 2019-03-26 | 福建农林大学 | A kind of light power bacteria reducing preservative film for packaged instant jellyfish |
| TWI736293B (en) * | 2020-05-29 | 2021-08-11 | 國立臺灣大學 | Method for forming chitin film |
| CN114805876A (en) * | 2022-05-13 | 2022-07-29 | 江南大学 | Preparation method of one-way water-blocking self-cleaning chitosan zein double-layer film |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108912700A (en) * | 2018-08-09 | 2018-11-30 | 云智前沿科技发展（深圳）有限公司 | A kind of composite material and preparation method and application with biological photoelectric dual function |
| CN109517226A (en) * | 2019-01-07 | 2019-03-26 | 福建农林大学 | A kind of light power bacteria reducing preservative film for packaged instant jellyfish |
| CN109517226B (en) * | 2019-01-07 | 2021-03-30 | 福建农林大学 | Photodynamic bacterium-reducing preservative film for packaging instant jellyfishes |
| TWI736293B (en) * | 2020-05-29 | 2021-08-11 | 國立臺灣大學 | Method for forming chitin film |
| US20210371601A1 (en) * | 2020-05-29 | 2021-12-02 | National Taiwan University | Method for forming chitin film |
| US11725083B2 (en) * | 2020-05-29 | 2023-08-15 | National Taiwan University | Method for forming chitin film |
| CN114805876A (en) * | 2022-05-13 | 2022-07-29 | 江南大学 | Preparation method of one-way water-blocking self-cleaning chitosan zein double-layer film |
| CN114805876B (en) * | 2022-05-13 | 2023-09-01 | 江南大学 | Preparation method of unidirectional water-blocking self-cleaning chitosan zein bilayer membrane |
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