CN106954861A - Ultrasonic wave prepares the method that zeins embeds resveratrol nano particle - Google Patents
Ultrasonic wave prepares the method that zeins embeds resveratrol nano particle Download PDFInfo
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Abstract
The method that zeins embeds resveratrol nano particle is prepared the invention discloses ultrasonic wave, belongs to and is related to food embedding techniques field.It is to be prepared from by the raw material of following parts by weight：Zeins：5 20 parts；Resveratrol：1 part.The present invention is applied with multi-mode ultrasonic wave during zeins embedding resveratrol, change the higher structure of zeins, open its structure, exposure active group, protein forms small aggregation, so as to promote each aggregation to provide basis by hydrophobic interaction formation nano particle for embedding bioactive ingredients.The zeins nano particle of the embedding resveratrol of the present invention has the advantages that good stability, good biocompatibility, slow-release time length, to contain rate high, can be applied to the multiple fields such as food, health products, medicine and cosmetics.
Description
Technical field
The present invention relates to food embedding techniques field, refer in particular to one kind and surpass using zeins as raw material, using multi-mode
Sonication prepares nano particle and the method embedded to resveratrol.
Background technology
The bioactive ingredients such as vitamin, unrighted acid, polyphenol compound or probiotics, with regulation human body life
The function of function is managed, the generation with preventing chronic disease can be delayed.However, above-mentioned active component is to food processing and stores
The factor such as temperature, oxygen, light, pH and metal ion is sensitive in journey, it is prone to aoxidize, isomerization, aggregation or the structure such as degrade change
Become, cause bioactivity to reduce or lose.Moreover, hydrophobicity and amphipathic active component solubility in water are very low.These
It greatly limit application of the bioactive ingredients in food and medicine industry.Bioactive ingredients are embedded
It is a kind of effective means for protecting, digesting and increasing bioavilability in food processing and storage to it.Natural life
Thing macromolecular (such as protein, polysaccharide) not only has high nutritive value, and with a variety of functional characteristics, is used extensively
Make the raw material in embedding techniques.
Zeins (zein) is once called as zein, and Japanese scholars are referred to as " prolamin ", as the term suggests belong to
Alcohol soluble protein class, it is named as zein by John Gorham within 1821.It is mixed by the polypeptide of many different peptide chains,
Molecular size, dissolubility and the electric charge of these polypeptides are different from, and McKinney proposed zeins first in 1958
(zein) two kinds of compositions of α-zein and β-zein are mainly included, alpha-alcohol soluble protein (α-zein) refers to the egg for being dissolved in 95% ethanol
In vain, the 80% of all alcohol soluble proteins is accounted for.The molten albumen of β -ol (β-zein) is dissolved in 60% ethanol and the ethanol insoluble in 95%, should
Albumen is comparatively unstable, easily occurs to be not present in retrogradation or gelling, industrially prepared zeins.With the molten egg of β -ol
In vain comparatively, alpha-alcohol soluble protein more lacks histidine, arginine, proline and methionine.Pomes proposes β -ol molten albumen point
Larger son amount is probably to be made up of alpha-alcohol soluble protein disulfide bond, because after it is hydrolyzed, gel electrophoresis occurs in that three
Main band, molecular weight is respectively：24000、22000、14000Da.
The unique structural property of zeins and amino acid composition determine that it is of low nutritive value, is difficult by organism
The characteristics of utilization, it is difficult to be used directly as raw-food material, cause its direct applied limitation in food production field.
But zeins have it is cohesives, bright, hydrophobic, hinder oxygen and the effects such as easy film forming, institute's film forming has certain toughness, and
The characteristic such as smooth, water-fast, oil resistant, anti-corrosion, has well in excipient substance, packaging material for food, biodegradable plastic field etc.
Potential application foreground, with great commercial value.Research such as Beck shows that zeins can be used as medicinal tablet
Coating.It can not be digested under one's belt, so it with regard to can be used to protecting effective component in stomach it is intact, but can
Dissolved in enteron aisle and slowly discharge its coatings (Beck M.I, Tomka I, Waysek E.Physico-chemical
charac-terization of zein as a film coating polymer-A direct compari-son with
with ethyl cellulose[J].Pharm.1996,14:137-150.).Li Xiuming etc. is at zeins (zein)
In with the addition of and gathered polypropylene carbonate (PPC), Zein-PPC laminated films are prepared by extrusion, PPC addition increases
The strong tensile strength and water resistance of composite membrane, the film of preparation for a kind of good degradable biological material (Li Xiuming, it is old
Open country, Wang Junyu waits .PPC to influence [J] Food Sciences of extrusion forming Zein-PPC laminated film properties, 2012, (23):6-
10.).Seung Yong Cho et al. have carried out the composite film packaging olive oil examination that zeins and soybean protein are made
Test, result of study shows, composite membrane can significantly slow oxidation and the corruption of olive oil, reached good resistance oxidation (Cho
SY,Lee SY,Rhee C.Edible oxygen barrier bilayer film pouches from corn zein
and soy protein isolate for olive oil packaging[J].LWT-Food Science and
Technology.2010,43(8):1234-1239.)。
Resveratrol is a kind of biological very strong natural Polyphenols anthraquinone terpene compound, also known as resvertrol, is swollen
The chemopreventive agent of knurl, is also to reduction platelet aggregation, prevention and treatment atherosclerosis, the chemistry of cardiovascular and cerebrovascular disease
Prophylactic, with effects such as anti-oxidant, antitumor, protection livers.But its property is unstable, poorly water-soluble, bioavilability
It is low, easily aoxidized under strong acid and strong base, illumination and higher temperature, and easily decompose broken in gastric environment after being taken in through human body
Badly lose activity, therefore a kind of carrier of resveratrol of research and development, it is encapsulated, to keep its activity and improve
Its bioavailability is particularly necessary.
Research finds that protein nanoparticles can play embedding and protective effect well to bioactive ingredients.Therefore
Domestic and foreign scholars have carried out the research that substantial amounts of protein is embedded to bioactive ingredients.Emulsion process is protein nanoparticles bag
The common method of bioactive ingredients is buried, the deficiency of this method shows that protein nanoparticles embed the process of bioactive ingredients
In need addition organic solvent, surfactant, glutaraldehyde cross-linking agent etc., the residual of these reagents makes protein nanoparticles
With certain toxicity；Solvent method and chemical crosslink technique is gone to be directed to the introducing of toxic cross-linking agents；Therefore these three methods are not eggs
The Perfected process of white matter nanoparticles embedded bioactive ingredients.Ionic cross-linking and self-assembly method prepare protein nano
Grain, mild condition, it is not necessary to poisonous crosslinking agent does not generate chemical combination key, only relies on non-covalent bond and be attached, thus extensive
Use.Particularly self-assembly method, for nutrient delivery system design, security etc. is relatively more preferably.But protein is after all
It is the large biological molecule of specific peculiar property, bioactive ingredients, protein molecule pair is embedded only by simple self-assembly method
The embedding efficiency of bioactive ingredients is low.
In order to solve this problem, the present invention introduces advanced multi-mode ultrasonic technology, it is desirable to which ultrasonic wave energy excites egg
Two kinds of biological macromolecule solns of white matter and polyphenol produce the resonant frequency matched with its own intrinsic frequency, produce crosslinking, obtain
A kind of high embedding efficiency, pH stabilizations, the protein nanoparticles of uniform particle diameter were obtained, the protein nanoparticles pair of gained are utilized
Bioactive ingredients are embedded and protected.
The content of the invention
To solve the above problems, the invention mainly embeds bioactive ingredients and multi-mode by protein nanoparticles
The technological means such as ultrasonication prepare zeins embedding resveratrol nano particle, and study it to resveratrol
Embedding effect.
The zeins nano particle of present invention embedding resveratrol, is by the raw material preparation of following parts by weight
Into：
Zeins：5-20 parts；
Resveratrol：1 part.
It is preferred that being prepared from by the raw material of following parts by weight：
Zeins：20 parts；
Resveratrol：1 part.
Wherein described zeins is commercially available prod.
Wherein described zeins is prepared as steps described below：
(1) cross 60 after maize yellow-powder is crushed using pulverizer to sieve, according to solid-liquid ratio 1:10 (mg/ml) add absolute ethyl alcohol；
(2) counter current ultrasonic wave aided bleaching 40min, then 4000r/min centrifuge 5min separate and collect supernatant；Filter cake is treated
Further ultrasonic extraction zeins；
(3) filter cake in step (2) is according to solid-liquid ratio 1:8 (mg/ml) add 80% ethanol, double frequency frequency sweep ultrasonic ripple auxiliary
Extract, then 4000r/min centrifuges 5min and separates and collects supernatant；
(4) above-mentioned supernatant pours into the sodium chloride solution that 2 times of volume mass concentration are 0.6%, reclaims zeins,
4 DEG C of standing 24h, then collect precipitation, wash repeatedly, after 40 DEG C of heated-air drying 4h, be ground into zeins finished product.
The design parameter of adverse current impulse ultrasound aided bleaching is 1~5s of pulse width, pulse spacing 1 wherein in step (2)
~5s, 50~80W/L of ultrasonic power density；Supersonic frequency 20kHz, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent.
Double frequency frequency sweep ultrasonic wherein described in step (3) fixes upper and lower plates spacing when working be 10cm, frequency sweep cycle
300s, intermittently than 1:1.2 (ultrasonic 10s, interval 12s), every piece of vibration plate power is 600W up and down.
Double frequency combination of frequency wherein described in step (3) is：33kHz/68kHz、33kHz/40kHz、33kHz/
28kHz；Most preferably good combination of frequency is 33kHz/68kHz.
The zeins nano particle of wherein described embedding resveratrol, is prepared into as steps described below
Arrive：
(1) zeins is dissolved into 70% ethanol, obtains zeins concentration for 0.06g/mL solution,
Magnetic agitation is to being completely dissolved；(4 DEG C) centrifugation 10min of low temperature under the conditions of 8000r/min；Take 0.75mL supernatants, vortex oscillation
Under the conditions of be distributed to dropwise in 2mL deionized waters；
(2) resveratrol will be entered into above-mentioned mixed solution under the conditions of lucifuge so that zeins and white lamb's-quarters
The mass ratio of reed alcohol is 5-20：1.
(3) multifrequency mode ultrasound processing is carried out simultaneously under the conditions of lucifuge, wherein described multiple frequency ultrasonic tupe is：
Three frequencies or double frequency or the processing of single-frequency synchronizing ultrasound, 80~120W/L of ultrasonic power density；Ultrasonic pulse working time 10s；
Interpulse period 3s, sonication treatment time is 40min.
Wherein three frequencies or double frequency or the ultrasonic frequency of single-frequency synchronizing ultrasound processing are combined as：20kHz、28kHz、
28/40kHz or 20/28/40kHz, preferably 20/28/40kHz.
(4) after ultrasound terminates, 6h is stood at room temperature, the zein solution of embedding resveratrol is produced, and spraying is dry
The zeins nano particle of resveratrol must be embedded after dry or freeze-drying.
The beneficial effects of the present invention are：
(1) present invention is applied with multi-mode ultrasonic wave during zeins embedding resveratrol, changes beautiful
The higher structure of rice alcohol soluble protein, opens its structure, exposure active group, and protein forms small aggregation, so as to promote
Each aggregation provides basis by hydrophobic interaction formation nano particle for embedding bioactive ingredients.
(2) preparation method of the zeins nano particle of resveratrol is embedded in the present invention, technological operation is simple,
Organic reagent, suitable industrialized production, and zeins cost of material are not directed in preparation process cheap, preparation technology letter
It is single.
(3) the zeins nano particle of embedding resveratrol of the invention has good stability, biocompatibility
Good, slow-release time length, the advantages of rate is high is contained, can be applied to the multiple fields such as food, health products, medicine and cosmetics.
(4) present invention is using maize yellow-powder as raw material, and separation and Extraction zeins therein is simultaneously white as embedding is prepared
The raw material of the zeins nano particle of veratryl alcohol, is conducive to the comprehensive utilization of corn processing accessory substance, while improving beautiful
The added value of cream-coloured powder.
Brief description of the drawings
Fig. 1 is the multi-mode ultrasonic wave biological processing equipment structure chart of the present invention, wherein 1,2,3 be ultrasonic vibrating plate, 4 be Sheng
Liquid device, 5 be water-bath, and 6 be temp probe, and 7 be circulating pump, and 8 be Computerized controller, and 9,10,11 be ultrasonic controller.
Fig. 2 is the equipment drawing of frequency sweep double-frequency ultrasound pretreatment unit, 1- ultrasounds pond, 2- thermometers, 3- thermostatted water bathing pools, 4-
Vibration plate under vibration plate on ultrasonic wave, 5- ultrasonic waves, 6- sample treatments region, 7- computer controllers, 8- supersonic generators.
Fig. 3 is adverse current ultrasound processing equipment structural representation, and 1 is energy-gathered ultrasonic probe, and 2 be ultrasonic wave controller, 3
It is charging aperture for ultrasonic cup type chamber, 4,5 be circulating pump, and 6 is contain liquid device, and 7 be discharging opening.
Embodiment
Used term, unless otherwise specified, typically has those of ordinary skill in the art usual in the present invention
The implication of understanding.The present invention is described in further detail with reference to specific embodiment, and with reference to data.It should be understood that these
Embodiment is of the invention solely for the purpose of illustration, rather than limits the scope of the present invention in any way.
Fig. 1 is multi-mode ultrasonic wave biological processing equipment of the invention, and the equipment is furnished with a Computerized controller 8,
Ultrasound works parameter (ultrasonic power density, frequency, pulse working time, intermittent time and processing total time) can be set to control respectively
Three ultrasonic controllers 9,10,11 are made, the ultrasonic vibrating plate 1,2,3 of three different frequencies is connected respectively, single-frequency/two can be achieved
Individual frequency/tri- frequency ultrasonic wave processing；Solution input to be processed will be needed to contain single-frequency/double frequency/multiple frequency ultrasonic is carried out in liquid device 4
Assistance enzymolysis is tested, and starts circulating pump 7 and solution is circulated.Oneself of solution temperature is realized by water-bath 5 and temp probe 6
Dynamic control.
The equipment drawing for the frequency sweep double-frequency ultrasound pretreatment unit that Fig. 2 uses for the present invention, is Jiangsu University's independent development.It is super
Acoustic generator 8 can send the ultrasonic wave for determining frequency and frequency sweep both of which, and separate unit supersonic generator power is 600w.In ultrasonic pond
Placement ultrasonic wave vibration plate upper plate 4 symmetrical above and below and ultrasonic wave lower plate 5, are controlled on ultrasonic wave vibration plate by supersonic generator 8 in 1
Plate 4 and ultrasonic wave lower plate 5；After the setting each parameter of ultrasonic wave of computer controller 7, supersonic generator 8 is controlled, sends and meets the requirements
Ultrasonic wave；3 be the thermostatted water bathing pool of equipment in the present invention, passes through the monitoring temperature, and being adjusted according to need of work in real time of thermometer 2
Save medium temperature；Need to liquid raw material to be processed be placed in sample sack to be placed in treatment fluid region 6 and carry out ultrasonication.
Fig. 3 is the adverse current ultrasound processing equipment of the present invention, and the equipment is furnished with ultrasonic wave controller 2, controls ultrasound parameter, poly-
The material that energy formula ultrasonic probe 1 enters ultrasonic cup type chamber 3 to charging aperture 4 is handled, and starts 5 pairs of circulating pump while ultrasonic
Feed liquid is circulated, and 6 is contain liquid device, and 7 be discharging opening.
Zeins in the present invention is commercially available prod (protein content is more than 90%).
Zeins in the present invention is prepared as steps described below：
(1) cross 60 after maize yellow-powder is crushed using pulverizer to sieve, according to solid-liquid ratio 1:10 (mg/ml) add absolute ethyl alcohol；
(2) counter current ultrasonic wave decolouring 40min, then 4000r/min centrifuge 5min separate and collect supernatant；Filter cake is treated into one
Walk ultrasonic extraction zeins；Design parameter is pulse width 5s, pulse spacing 5s, ultrasonic power density 80W/L；Ultrasound
Frequency 20kHz, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent.
(3) filter cake in step (2) is according to solid-liquid ratio 1:8 (mg/ml) add 80% ethanol, double frequency frequency sweep ultrasonic ripple auxiliary
Extract, then 4000r/min centrifuges 5min and separates and collects supernatant；Upper and lower plates spacing is fixed when double frequency frequency sweep ultrasonic works is
10cm, frequency sweep cycle 300s, intermittently than 1:1.2 (ultrasonic 10s, interval 12s), every piece of vibration plate power is 600W up and down；Double frequency is frequently
Rate is combined as：33kHz/68kHz.
(4) above-mentioned supernatant pours into the sodium chloride solution that 2 times of volume mass concentration are 0.6%, reclaims zeins,
4 DEG C of standing 24h, then collect precipitation, wash repeatedly, after 40 DEG C of heated-air drying 4h, be ground into zeins finished product, measure
Wherein protein content is 90.5%.
Embodiment 1-4 (is not added with ultrasound)
(1) zeins is dissolved into 70% ethanol, obtains zeins concentration for 0.06g/mL solution,
Magnetic agitation is to being completely dissolved；(4 DEG C) centrifugation 10min of low temperature under the conditions of 8000r/min；Take 0.75mL supernatants, vortex oscillation
Under the conditions of be distributed to dropwise in 2mL deionized waters；
(2) resveratrol will be entered into above-mentioned mixed solution under the conditions of lucifuge, zeins and resveratrol
Mass ratio be 5:1.
(3) 6h is stood at room temperature, the zein solution of embedding resveratrol is produced, and determines white as steps described below
Veratryl alcohol envelop rate, the Resveratrol content that dissociates is determined:The zein solution of cladding resveratrol is taken, through super filter tube
(molecular cut off 10kD) centrifuges 20min under 8 000 × g, takes the μ L of filtrate 500 constant volumes in 25mL volumetric flasks, determines A306
The light absorption value at place, and calculate free resveratrol quality.
The envelop rate and load factor of resveratrol are calculated according to following formula：
The envelop rate (%) of the resveratrol=white black false hellebore in (resveratrol gross mass-free resveratrol quality) × 100/
Alcohol gross mass；
The load factor (%) of resveratrol=(resveratrol gross mass-free resveratrol quality) × 100/ sample is total
Quality.
(4) the zeins nano particle of resveratrol must be embedded after being spray-dried or be freeze-dried.
Embodiment 2-4 preparation process be the same as Example 1, simply zeins/resveratrol quality is different, is specifically shown in Table
1 different zeins/influences of the resveratrol mass ratio to resveratrol envelop rate and load factor.
By comparative example 1-4 differences zeins/resveratrol mass ratio in table 1 to resveratrol envelop rate
Influence with load factor is as can be seen that with the amount increase of zeins, the envelop rate of resveratrol is gradually reduced, still
When zeins/resveratrol mass ratio is 20:When 1, the envelop rate of resveratrol is increased again, has reached 55.6%.
Different zeins/influences of the resveratrol mass ratio to resveratrol envelop rate and load factor of table 1
Embodiment 5-10 (plus ultrasound)
(1) zeins is dissolved into 70% ethanol, obtains zeins concentration for 0.06g/mL solution,
Magnetic agitation is to being completely dissolved；(4 DEG C) centrifugation 10min of low temperature under the conditions of 8000r/min；Take 0.75mL supernatants, vortex oscillation
Under the conditions of be distributed to dropwise in 2mL deionized waters；
(2) resveratrol will be entered into above-mentioned mixed solution under the conditions of lucifuge, zeins and resveratrol
Mass ratio be 10:1.
(3) multifrequency mode ultrasound processing is carried out simultaneously under the conditions of lucifuge, wherein described multiple frequency ultrasonic tupe is：
Three frequencies or double frequency or the processing of single-frequency synchronizing ultrasound, ultrasonic power density 120W/L；Ultrasonic pulse working time 10s；Pulse
Intermittent time 3s.Wherein synchronizing ultrasound processing ultrasonic frequency be：28kHz.
(4) 6h is stood at room temperature, the zein solution of embedding resveratrol is produced, and determines white as steps described below
Veratryl alcohol envelop rate, the Resveratrol content that dissociates is determined:The zein solution of cladding resveratrol is taken, through super filter tube
(molecular cut off 10kD) centrifuges 20min under 8 000 × g, takes the μ L of filtrate 500 constant volumes in 25mL volumetric flasks, determines A306
The light absorption value at place, and calculate free resveratrol quality.
The envelop rate and load factor of resveratrol are calculated according to following formula：
The envelop rate (%) of the resveratrol=white black false hellebore in (resveratrol gross mass-free resveratrol quality) × 100/
Alcohol gross mass；
The load factor (%) of resveratrol=(resveratrol gross mass-free resveratrol quality) × 100/ sample is total
Quality.
(5) the zeins nano particle of resveratrol must be embedded after being spray-dried or be freeze-dried.
Embodiment 6-8 preparation process be the same as Example 5, simply ultrasound parameter is slightly changed, the different ultrasound mode dialogues of table 2
The influence of veratryl alcohol envelop rate and load factor.
Influence of the different ultrasound modes of table 2 to resveratrol envelop rate and load factor
By comparative example 5-8 differences ultrasound mode in table 2 to the white black false hellebore of zeins nanoparticles embedded
The envelop rate of alcohol and the influence of load factor, it can be found that ultrasonication can significantly improve zeins nano particle bag
The envelop rate of resveratrol is buried, compared with the control (not ultrasonic), 28kHz single-frequency ultrasonication can make the bag of resveratrol
Envelope rate improves 70.4%, and the processing of 20/28/40kHz multi-frequency ultrasonic then makes the envelop rate of resveratrol improve 88.3%.
Claims (8)
1. embed the zeins nano particle of resveratrol, it is characterised in that by the raw material preparation of following parts by weight
Into：
Zeins：5-20 parts；
Resveratrol：1 part.
2. the zeins nano particle of embedding resveratrol according to claim 1, it is characterised in that by following
The raw material of parts by weight is prepared from：
Zeins：20 parts；
Resveratrol：1 part.
3. the zeins nano particle of embedding resveratrol according to claim 1, it is characterised in that wherein institute
The zeins stated is prepared as steps described below：
（1）Maize yellow-powder is crossed 60 after being crushed using pulverizer and sieved, according to solid-liquid ratio 1:10（mg/ml）Add absolute ethyl alcohol；
（2）Counter current ultrasonic wave aided bleaching 40min, then 4000r/min centrifuge 5min separate and collect supernatant；Filter cake is treated into one
Walk ultrasonic extraction zeins；
（3）Step（2）In filter cake according to solid-liquid ratio 1:8（mg/ml）Add 80% ethanol, double frequency frequency sweep ultrasonic ripple assisted extraction,
Then 4000r/min centrifuges 5min and separates and collects supernatant；
（4）Above-mentioned supernatant pours into the sodium chloride solution that 2 times of volume mass concentration are 0.6%, reclaims zeins, 4 DEG C quiet
24h is put, precipitation is then collected, washes repeatedly, after 40 DEG C of heated-air drying 4h, be ground into zeins finished product.
4. it is according to claim 3 embedding resveratrol zeins nano particle, it is characterised in that wherein its
Middle step（2）The design parameter of middle adverse current impulse ultrasound aided bleaching is 1 ~ 5s of pulse width, 1 ~ 5s of pulse spacing, ultrasonic power
The W/L of density 50 ~ 80；The kHz of supersonic frequency 20, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent.
5. the zeins nano particle of embedding resveratrol according to claim 3, it is characterised in that wherein walk
Suddenly（3）Described in the work of double frequency frequency sweep ultrasonic when to fix upper and lower plates spacing be 10cm, frequency sweep cycle 300s, intermittently than 1:1.2
（Ultrasonic 10s, interval 12s）, every piece of vibration plate power is 600W up and down.
6. the zeins nano particle of embedding resveratrol according to claim 3, it is characterised in that wherein walk
Suddenly（3）Described in double frequency combination of frequency be： 33 kHz/ 68kHz、33kHz/40kHz、33 kHz/28 kHz；It is most preferably good
Combination of frequency is 33 kHz/68 kHz.
7. the preparation method of the zeins nano particle of the embedding resveratrol described in claim 1-6, its feature exists
In carrying out as steps described below：
（1）Zeins is dissolved into 70% ethanol, zeins concentration is obtained for 0.06g/mL solution, magnetic force
Stirring is to being completely dissolved；Refrigerated centrifuge 10min under the conditions of 8000 r/min；Take under the conditions of 0.75 mL supernatants, vortex oscillation by
Drop is distributed in 2 mL deionized waters；
（2）Resveratrol will be entered into above-mentioned mixed solution under the conditions of lucifuge so that zeins and resveratrol
Mass ratio be 5-20：1；
（3）Multifrequency mode ultrasound processing is carried out simultaneously under the conditions of lucifuge, wherein described multiple frequency ultrasonic tupe is：Three frequencies
Or double frequency or the processing of single-frequency synchronizing ultrasound, the W/L of ultrasonic power density 80 ~ 120；The s of ultrasonic pulse working time 10；Pulse
The s of intermittent time 3, sonication treatment time is 40min；
（4）After ultrasound terminates, 6h is stood at room temperature, produce embedding resveratrol zein solution, spray drying or
The zeins nano particle of resveratrol must be embedded after person's freeze-drying.
8. the preparation method of the zeins nano particle of the embedding resveratrol described in a claim 7, its feature exists
In step（3）In three frequency or double frequency or single-frequency synchronizing ultrasound processing ultrasonic frequency be combined as：20kHz、28 kHz、
28/40 kHz or 20/28/40 kHz, preferably 20/28/40 kHz.
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