CN103549110B - Method for extracting water-soluble rice bran proteins - Google Patents
Method for extracting water-soluble rice bran proteins Download PDFInfo
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- CN103549110B CN103549110B CN201310536586.9A CN201310536586A CN103549110B CN 103549110 B CN103549110 B CN 103549110B CN 201310536586 A CN201310536586 A CN 201310536586A CN 103549110 B CN103549110 B CN 103549110B
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- rice bran
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- soluble rice
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Abstract
The invention discloses a method for extracting water-soluble rice bran proteins. The method comprises the following steps: (1) preprocessing degreased rice bran; (2) ultrasonically processing; (3) carrying out protease hydrolysis; (4) carrying out centrifugal separation, and washing sediments; and (5) carrying out freeze-drying. The rice bran proteins extracted by using the method are relatively high in yield, high in purity and good in water solubility.
Description
Technical field
The present invention relates to a kind of extracting method of rice bran protein, especially relate to a kind of method adopting ultrasonic assistant protease to extract soluble rice bran.
Background technology
China is eating rice production big country, produce rice per year and reach more than 1.8 hundred million tons, and rice bran is as Rice producing byproduct, annual production is also more than 1,000 ten thousand tons of (Yao Huiyuan, Zhou Sumei, Wang Li. the exploitation [J] of rice bran and rice bran protein matter. Wuxi light industry college journal, 2002,21 (3): 312 ~ 316).Rice bran is a kind of resource having deep development and be worth, especially rice bran protein has low-allergen, its amino acid composition recommends pattern close with FAO/WHO, be of high nutritive value, can compare favourably with animal protein, exploitation (Chen Yiyong, the Wang Wei of infant food can be widely used in, Shen Zonggen, Deng. rice bran and rice bran protein deep development present situation [J]. grain processing, 2006,3 (5): 24 ~ 25).
At present, the extracting method of rice bran protein mainly contain alkaline process, Physical, enzyme process etc. (Zhu Lei, Wang Xuede, Yu Xinguo etc., comprehensive study progress [J] of rice bran protein. Chinese oil, 2013(2): 81-83).The easy sex change of rice bran protein prepared by alkali process and recovery rate is low, Physical recovery rate is low, equipment investment higher (Jiang Aizhi, Wang Yan. the progress [J] of rice bran protein extracting method. processing of farm products-innovation version, 2009 (10): 35-37).Enzyme process mainly adopts protease, in order to reach the good functional and trophism of rice bran protein, when using protease, need to control lower degree of hydrolysis (DH), the difficulty of extraction process greatly increases (Ma Haile, Zhang Lianbo. the Pulse ultrasonic-assistant extraction technology [J] of rice bran protein. Jiangsu University's journal (natural science edition), 2006 (1): 14 ~ 17).The rice bran protein that above three kinds of methods are extracted is water-soluble poor, be unfavorable for digesting and assimilating of human body, and recovery rate is low.
Summary of the invention
Technical problem to be solved by this invention overcomes the deficiencies in the prior art, take defatted rice bran as raw material, provides the extracting method of the soluble rice bran that a kind of preparation efficiency is high, and gained rice bran protein purity is high, well water-soluble.
The technical scheme that the present invention solves the employing of its technical problem is that a kind of extracting method of soluble rice bran, comprises the following steps:
(1) defatted rice bran pretreatment: 20-100 mesh sieve crossed by defatted rice bran, adds distilled water according to solid-liquid ratio 1:10-40 (g:mL) (preferred 1g:20mL), adopts delicatessen food level alkali salt that pH is adjusted to 7-10, obtains defatted rice bran aqueous mixtures;
(2) ultrasonic wave process: the defatted rice bran aqueous mixtures of step (1) gained is placed in beaker, and beaker is placed in water-bath, bath temperature keeps 10 ~ 30 DEG C (preferably 20 DEG C), ultrasonic pulse process, ultrasonic power is the preferred 600W of 400-1200W(), pulse working time 1-4 s, pulse interval 1-4 s, total processing time is the preferred 60min of 30 ~ 100 min(), this process will adopt pH meter Real-Time Monitoring to make pH be stabilized in 7-10;
(3) protease hydrolytic: the pH of the mixture after first adopting food grade hydrochloric acid step (2) to be processed is adjusted to 5-9, the protease adding the 2-4% being equivalent to defatted rice bran material quality carries out enzyme digestion reaction, hydrolysis temperature is 25-60 DEG C (preferably 45 DEG C), and the time is 2-4 h;
(4) centrifugation, washing of precipitate: the solution obtained after step (3) being reacted goes out after enzyme 5-20 min at 70-100 DEG C, centrifugal 10-20 min under 2500 ~ 3500 r/min, collect supernatant, adopt food grade salt acid for adjusting pH to 3.0-5.5, again centrifugal, gained precipitation is soluble rice bran;
(5) freeze drying: freeze drying process is carried out to the albumen precipitation soluble rice bran that step (4) obtains; Pre-freeze speed is 1-5 DEG C/min, and hothouse vacuum is 50 ~ 500 Pa, and-30 ~-10 DEG C time, pre-freeze 10-30 min, is dried to moisture≤8 %, obtains final product.
Further, the food grade alkaline salts in described step (1) is sodium carbonate.
Further, the protease in described step (3) is the bent protease of rice, and the enzyme work of described protease is 1 × 10
4-4 × 10
4u/g.
Rice bran protein recovery rate and functional characteristic evaluation method:
1. the mensuration of rice bran protein content and recovery rate
Protein content employing micro-Kjeldahl (AOAC. Official methods of analysis [M]. 15th ed. Washington D C:Association of Official Analytical Chemists, 1990) measure (conversion coefficient is 5.95); Recovery rate E=(W
1/ W
0) × 100%, W in formula
1for protein quality (g) in extract, W
0for protein quality (g) in rice bran.
2. the water-soluble evaluation method of rice bran protein
Represent with the solubility of rice bran protein, in 50mL ultra-pure water, add the tested rice bran protein quality sample of 1g, be incubated in 25 DEG C of thermostat water baths, and use agitator to stir 1h, suspension centrifugal (3000r/min) 20min, in supernatant, protein content biuret method measures.Be calculated as follows dissolubility:
The ultrasonic assistant protease of the present invention extracts rice bran protein and has been coupled alkaline process, Physical, enzyme method technique, substantially increase extraction efficiency, and the rice bran protein extracted is water-soluble is better than the water-soluble of the rice bran protein that alone alkaline process, Physical or enzyme method technique obtain.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
The extracting method of the soluble rice bran of the present embodiment, comprises the steps:
(1) defatted rice bran pretreatment: defatted rice bran is crossed 40 mesh sieves, take defatted rice bran 100g, the defatted rice bran that will sieve loads in beaker, add 2L distilled water, FE20 laboratory pH meter is used to measure the pH of solution, and adopt the food-grade sodium carbonate liquor of 1mol/L that pH is adjusted to 9, obtain defatted rice bran aqueous mixtures;
(2) ultrasonic wave process: the beaker that defatted rice bran aqueous mixtures is housed is placed in DZKW-4 electronic thermostatic water-bath, bath temperature is stabilized in 20 DEG C, then ultrasonic probe is put into beaker, carry out impulse ultrasound extraction, ultrasonic power is 500W, impulse ultrasound interval 2s, pulse working time 2s, extraction time is 60min; Period wants the pH value of the real time measure solution, makes it keep being stabilized in pH=9;
(3) protease hydrolytic: after ultrasonic wave process, the pH of the mixed solution after utilizing 1mol/L hydrochloric acid step (2) to be processed is adjusted to 7.5, and the bent protease of rice adding 2g reacts, and water-bath stability contorting is at 40 DEG C, and the reaction time is 2h;
(4) centrifugation, washing of precipitate: after enzymolysis process terminates, be adjusted to 80 DEG C by bath temperature, keep 10min, solution is gone out ferment treatment, then carry out centrifugal treating with RJ-TDL-40B low speed desk centrifuge to solution, rotating speed controls at 3000r/min, time 10min, after centrifugal end, collect supernatant, adopt 1mol/L hydrochloric acid that supernatant is regulated pH to 4.5, albumen is precipitated, and again carry out centrifugal treating, gained precipitation is soluble rice bran;
(5) freeze drying: carry out freeze drying process to the albumen precipitation soluble rice bran that step (4) obtains, pre-freeze speed is 3 DEG C/min, and hothouse vacuum is 200 Pa, and-20 DEG C time, pre-freeze 20 min is dry, and being dried to moisture is 5%, to obtain final product.
Ultrasonic assistant enzyme process extracts the evaluation of soluble rice bran: adopt micro-Kjeldahl, the recovery rate calculating rice bran protein is 79.21%, and purity is 80.34%, and the rice bran protein dissolubility of gained is 99.13%.
Embodiment 2
The extracting method of the soluble rice bran of the present embodiment, comprises the following steps:
(1) defatted rice bran pretreatment: defatted rice bran is crossed 60 mesh sieves, take the defatted rice bran 200g that sieves, the defatted rice bran that will sieve loads in beaker, add 2L distilled water, pH meter is adopted to measure the pH of solution, and adopt the food sodium carbonate liquor of 1mol/L that pH is adjusted to 8, obtain defatted rice bran aqueous mixtures;
(2) ultrasonic wave process: the beaker that step (1) is equipped with defatted rice bran aqueous mixtures is placed in electronic thermostatic water-bath, bath temperature is stabilized in 25 DEG C, then impulse ultrasound is adopted to carry out ultrasonic assistant extraction, ultrasonic power is 600W, impulse ultrasound time interval 1.8s, pulse working time 2.5s, extraction time is 70min, pH will be made to be stabilized in 8 by adopting pH meter Real-Time Monitoring during ultrasonic process;
(3) protease hydrolytic: after ultrasonic wave process, utilize the pH of mixture of 1mol/L hydrochloric acid just after step (2) process to be adjusted to 7, the bent protease of rice adding 6g carries out enzyme digestion reaction, and water-bath stability contorting is at 50 DEG C, and the reaction time is 1.5h;
(4) centrifugation, washing of precipitate: after enzymolysis process, bath temperature is adjusted to 100 DEG C, keeps 8min, solution is gone out ferment treatment, then with low speed desk centrifuge, centrifugal treating is carried out to solution, rotating speed controls at 2500r/min, time 15min, after centrifugal end, collect supernatant, adopt 1mol/L hydrochloric acid that supernatant is regulated pH to 4, albumen is precipitated, and again carries out centrifugal treating; Gained precipitation is soluble rice bran;
(5) freeze drying: freeze drying process is carried out to the albumen precipitation soluble rice bran after centrifugal, pre-freeze speed is 2 DEG C/min, and hothouse vacuum is 100 Pa, and-20 DEG C time, pre-freeze 30 min is dry; Being dried to moisture is 6%, to obtain final product.
Ultrasonic assistant enzyme process extracts the evaluation of soluble rice bran: adopt micro-Kjeldahl, the recovery rate calculating rice bran protein is 75.45%, and purity is 78.90%, and the rice bran protein dissolubility of gained is 99.20%.
Claims (8)
1. an extracting method for soluble rice bran, is characterized in that, comprises the following steps:
(1) defatted rice bran pretreatment: 20-100 mesh sieve crossed by defatted rice bran, adds distilled water according to solid-liquid ratio 1:10-40, adopts alkali salt that pH is adjusted to 7-10, obtains defatted rice bran aqueous mixtures;
(2) ultrasonic wave process: the defatted rice bran aqueous mixtures of step (1) gained is placed in beaker, and beaker is placed in water-bath, bath temperature keeps 10 ~ 30 DEG C, ultrasonic pulse process, ultrasonic power is 400-1200W, pulse working time 1-4 s, pulse interval 1-4 s, total processing time is 30 ~ 100 min, and this process will adopt pH meter Real-Time Monitoring to make pH be stabilized in 7-10;
(3) protease hydrolytic: the pH of the mixture after first adopting food grade hydrochloric acid step (2) to be processed is adjusted to 5-9, and the protease adding the 2-4% being equivalent to defatted rice bran material quality carries out enzyme digestion reaction, and hydrolysis temperature is 25-60 DEG C, and the time is 2-4 h;
Described protease is the bent protease of rice;
(4) centrifugation, washing of precipitate: the solution obtained after step (3) being reacted goes out after enzyme 5-20 min at 70-100 DEG C, centrifugal 10-20 min under 2500 ~ 3500 r/min, collect supernatant, adopt food grade salt acid for adjusting pH to 3.0-5.5, centrifugal gained precipitation is soluble rice bran again;
(5) freeze drying: freeze drying process is carried out to the albumen precipitation soluble rice bran that step (4) obtains; Pre-freeze speed is 1-5 DEG C/min, and hothouse vacuum is 50 ~ 500 Pa, and-30 ~-10 DEG C time, pre-freeze 10-30 min, is dried to moisture≤8 %, obtains final product.
2. the extracting method of soluble rice bran according to claim 1, is characterized in that, in step (1), adds distilled water according to solid-liquid ratio 1g:20mL.
3. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (2), bath temperature keeps 20 DEG C.
4. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (2), ultrasonic power is 600W.
5. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (2), ultrasonic total processing time is 60min.
6. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (3), hydrolysis temperature is 45 DEG C.
7. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, the food grade alkaline salts in described step (1) is sodium carbonate.
8. the extracting method of soluble rice bran according to claim 1 and 2, is characterized in that, in step (3), the enzyme work of described protease is 1 × 10
4-4 × 10
4u/g.
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| CN104938764B (en) * | 2015-05-25 | 2020-06-23 | 浙江大学 | Method for extracting rice bran protein |
| CN105475607A (en) * | 2015-11-06 | 2016-04-13 | 武汉轻工大学 | Method for extracting protein from low-temperature rice bran meal |
| CN112481022A (en) * | 2020-12-22 | 2021-03-12 | 霍邱县敏东米业有限公司 | Method for synchronously preparing rice bran oil and high-purity active peptide by using rice bran |
| CN112970933A (en) * | 2021-03-30 | 2021-06-18 | 江西恒顶食品有限公司 | Rice bran protein extraction method |
| CN113176361B (en) * | 2021-06-30 | 2021-11-16 | 中国农业科学院蜜蜂研究所 | Identification method and application of bee pollen allergenic protein |
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| CN101058822A (en) * | 2007-05-17 | 2007-10-24 | 中兴能源技术（武汉）有限公司 | Method of extracting rice bran polysaccharide and rice bran protein |
| CN102115690A (en) * | 2011-01-12 | 2011-07-06 | 武汉百信食品有限公司 | Method for comprehensively utilizing rice bran |
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| KR101079030B1 (en) * | 2009-06-15 | 2011-11-02 | 대상 주식회사 | Process for producing rice bran-derived protein hydrolysate having high glutaminc acid contents |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101058822A (en) * | 2007-05-17 | 2007-10-24 | 中兴能源技术（武汉）有限公司 | Method of extracting rice bran polysaccharide and rice bran protein |
| CN102115690A (en) * | 2011-01-12 | 2011-07-06 | 武汉百信食品有限公司 | Method for comprehensively utilizing rice bran |
Non-Patent Citations (3)
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| SHANHU TANG,etc.Protein Extraction from Heat-Stabilized Defatted Rice Bran. 1.Physical Processing and Enzyme Treatments.《J. Agric. Food Chem》.2002,第50卷(第25期),第7444-7448页. * |
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Effective date of registration: 20171206 Address after: Du Zhen Gang village 423000 foot Chenzhou city in Hunan province Suxian District Qi Feng (former Gang Jiao township government office) Patentee after: Hunan Feng Chu Food Technology Co., Ltd. Address before: 410004 Shaoshan South Road, Hunan, No. 498, No. Patentee before: Central South University of Forestry and Technology |
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Address after: 423000 Hunan province Chenzhou city Suxian District Qifeng Village town foot village (formerly Gang Kong township government office building) Patentee after: Hunan Fengchu Food Co.,Ltd. Address before: 423000 Hunan province Chenzhou city Suxian District Qifeng Village town foot village (formerly Gang Kong township government office building) Patentee before: Hunan Feng Chu Food Technology Co.,Ltd. |