CN104351464A - Preparation method of soybean peptide powder - Google Patents
Preparation method of soybean peptide powder Download PDFInfo
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- CN104351464A CN104351464A CN201410709970.9A CN201410709970A CN104351464A CN 104351464 A CN104351464 A CN 104351464A CN 201410709970 A CN201410709970 A CN 201410709970A CN 104351464 A CN104351464 A CN 104351464A
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Abstract
The invention discloses a preparation method of soybean peptide power. The soybean peptide powder is prepared from soybean meal by steps of fermenting the soybean meal by virtue of high-activity dried yeast, enzymatically hydrolyzing fermentation liquor through trypsin, carrying out high-temperature enzyme deactivation and centrifugal treatment on enzymatic hydrolysate to obtain clear liquid, and spray-drying to obtain the soybean peptide power. The soybean peptide power disclosed by the invention is directly prepared from soybean meal by fermenting with addition of the high-activity dried yeast, enzymatically hydrolyzing through trypsin and spray-drying; the soybean peptide power is broad in raw material source, gentle in technological condition, less in damage to soybean peptide, environmentally friendly and free from pollution; the prepared product is in the powdery form, and is convenient for carrying and deep processing.
Description
Technical field
The present invention relates to technical field of bioengineering, be specifically related to a kind of preparation method of soy peptide powder.
Background technology
Soybean Peptide is with soybean or derivatives thereof, and through acid system or enzymatic hydrolysis, the refining and mixtures of polypeptides that obtains, based on small-molecular peptides, also containing compositions such as a small amount of large molecular peptide, free amino acid, carbohydrate and inorganic salts, molecular weight mainly concentrates on less than 1000.Soybean Peptide, as excellent health food, not only has reducing blood lipid, easy to digest, easy absorption, promotes energetic supersession and anti-obesic action, and can dispelling fatigue, strengthen the flexible and antiallergy of muscle etc.This product integrates nutritive peculiarity, health care characteristics and processing characteristics, has multiple regulatory function to body, is widely used at food and health products production field.There is the shortcomings such as preparation cost is high, complex process, length consuming time in existing soybean peptide production method, needs to be further improved, to obtain Soybean Peptide product of high quality and at a reasonable price mostly.
Summary of the invention
The technical problem to be solved in the present invention is to provide that a kind of technique is simple, preparation cost is low, the preparation method of short soy peptide powder consuming time.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions:
A preparation method for soy peptide powder take soy meal as raw material, and by fermentation, zymotic fluid enzymolysis, high temperature go out the clear liquid obtained after enzyme, centrifugal treating more spray-dried rear obtained soy peptide powder.
In technique scheme:
The process of described fermentation is soy meal and water are mixed by the weight ratio of 1:2 ~ 5, and the amount of adding 0.001 ~ 0.1 gram with every gram of raw material adds high activity dried yeast, at room temperature sealing and fermenting 1 ~ 3 day.
The process of described enzymolysis is add trypsase, enzymolysis 0.5 ~ 4 hour under the enzymatic hydrolysis condition of temperature 40 ~ 60 DEG C, pH 7.5 ~ 8.5 with the amount of every gram of soy meal interpolation 100 ~ 1000U in zymotic fluid.
Described high temperature goes out the process of enzyme for keeping 10 ~ 20 minutes after enzymolysis liquid is warming up to 85 ~ 100 DEG C.
Centrifugal rotational speed during described centrifugal treating is 2000 ~ 6000r/min.
Described spray-dired process is that the Fructus Hordei Germinatus adding its weight 2% ~ 3% in clear liquid is stuck with paste, carry out drying again after the cycloheptaamylose of 0.5% ~ 1.5%; EAT time dry is 175 ~ 185 DEG C, and temperature of outgoing air is 70 ~ 80 DEG C.
The invention has the beneficial effects as follows:
The present invention is directly raw material with soy meal, its wide material sources, cheap, after high activity dried yeast fermentation, trypsin digestion and spraying dry, product is obtained during preparation, process conditions are gentle, few to the destruction of Soybean Peptide, environmentally friendly, pollution-free, obtained product is powdery, easy to carry, be beneficial to use for further processing.
Detailed description of the invention
Be described further with embodiment below, but the present invention is not limited to these embodiments.
Embodiment 1
The preparation method of soy peptide powder, comprises the following steps:
1) soy meal and water are pressed the weight ratio mixing of 1:3.5, the amount of adding 0.01 gram with every gram of raw material adds high activity dried yeast, at room temperature sealing and fermenting 2 days;
2) in zymotic fluid, trypsase is added, enzymolysis 2.5 hours under the enzymatic hydrolysis condition of temperature 53 DEG C, pH 8.0 with the amount of every gram of soy meal interpolation 800U; 15 minutes are kept with enzymatic activity of going out after enzymolysis liquid being warming up to 95 DEG C; Be that 4000r/min carries out centrifugal treating with centrifugal rotational speed, get its clear liquid;
3) in clear liquid, add the Fructus Hordei Germinatus paste of its weight 2.5%, the cycloheptaamylose of 1%, after mixing, carry out spraying dry; EAT time dry is 170 DEG C, and temperature of outgoing air is 75 DEG C.
Embodiment 2
The preparation method of soy peptide powder, comprises the following steps:
1) soy meal and water are pressed the weight ratio mixing of 1:2, the amount of adding 0.01 gram with every gram of raw material adds high activity dried yeast, at room temperature sealing and fermenting 3 days;
2) in zymotic fluid, trypsase is added, enzymolysis 0.5 hour under the enzymatic hydrolysis condition of temperature 50 C, pH 8.5 with the amount of every gram of soy meal interpolation 100U; 20 minutes are kept with enzymatic activity of going out after enzymolysis liquid being warming up to 90 DEG C; Be that 2000r/min carries out centrifugal treating with centrifugal rotational speed, get its clear liquid;
3) in clear liquid, add the Fructus Hordei Germinatus paste of its weight 2.5%, the cycloheptaamylose of 1.5%, after mixing, carry out spraying dry; EAT time dry is 175 DEG C, and temperature of outgoing air is 75 DEG C.
Embodiment 3
The preparation method of soy peptide powder, comprises the following steps:
1) soy meal and water are pressed the weight ratio mixing of 1:4, the amount of adding 0.1 gram with every gram of raw material adds high activity dried yeast, at room temperature sealing and fermenting 1 day;
2) in zymotic fluid, trypsase is added, enzymolysis 2.5 hours under the enzymatic hydrolysis condition of temperature 60 C, pH 7.5 with the amount of every gram of soy meal interpolation 800U; 10 minutes are kept with enzymatic activity of going out after enzymolysis liquid being warming up to 100 DEG C; Be that 4000r/min carries out centrifugal treating with centrifugal rotational speed, get its clear liquid;
3) in clear liquid, add the Fructus Hordei Germinatus paste of its weight 3%, the cycloheptaamylose of 0.5%, after mixing, carry out spraying dry; EAT time dry is 180 DEG C, and temperature of outgoing air is 80 DEG C.
Embodiment 4
The preparation method of soy peptide powder, comprises the following steps:
1) soy meal and water are pressed the weight ratio mixing of 1:5, the amount of adding 0.001 gram with every gram of raw material adds high activity dried yeast, at room temperature sealing and fermenting 3 days;
2) in zymotic fluid, trypsase is added, enzymolysis 4 hours under the enzymatic hydrolysis condition of temperature 40 DEG C, pH 8.0 with the amount of every gram of soy meal interpolation 1000U; 15 minutes are kept with enzymatic activity of going out after enzymolysis liquid being warming up to 85 DEG C; Be that 6000r/min carries out centrifugal treating with centrifugal rotational speed, get its clear liquid;
3) in clear liquid, add the Fructus Hordei Germinatus paste of its weight 2%, the cycloheptaamylose of 1%, after mixing, carry out spraying dry; EAT time dry is 185 DEG C, and temperature of outgoing air is 70 DEG C.
Claims (6)
1. a preparation method for soy peptide powder, is characterized in that: take soy meal as raw material, and by fermentation, zymotic fluid enzymolysis, high temperature go out the clear liquid obtained after enzyme, centrifugal treating more spray-dried rear obtained soy peptide powder.
2. preparation method according to claim 1, it is characterized in that: the process of described fermentation is soy meal and water are mixed by the weight ratio of 1:2 ~ 5, the amount of adding 0.001 ~ 0.1 gram with every gram of raw material adds high activity dried yeast, at room temperature sealing and fermenting 1 ~ 3 day.
3. preparation method according to claim 1, it is characterized in that: the process of described enzymolysis is add trypsase, enzymolysis 0.5 ~ 4 hour under the enzymatic hydrolysis condition of temperature 40 ~ 60 DEG C, pH 7.5 ~ 8.5 with the amount of every gram of soy meal interpolation 100 ~ 1000U in zymotic fluid.
4. preparation method according to claim 1, is characterized in that: described high temperature goes out the process of enzyme for keeping 10 ~ 20 minutes after enzymolysis liquid is warming up to 85 ~ 100 DEG C.
5. preparation method according to claim 1, is characterized in that: centrifugal rotational speed during described centrifugal treating is 2000 ~ 6000r/min.
6. preparation method according to claim 1, is characterized in that: the described spray-dired Fructus Hordei Germinatus for adding its weight 2% ~ 3% in clear liquid is stuck with paste, carry out drying again after the cycloheptaamylose of 0.5% ~ 1.5%; EAT time dry is 175 ~ 185 DEG C, and temperature of outgoing air is 70 ~ 80 DEG C.
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| CN201410709970.9A CN104351464A (en) | 2014-11-28 | 2014-11-28 | Preparation method of soybean peptide powder |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782768A (en) * | 2015-04-03 | 2015-07-22 | 傅水娟 | Functional drink food and preparation method thereof |
| CN105104756A (en) * | 2015-09-15 | 2015-12-02 | 江苏省苏微微生物研究有限公司 | Mushroom bran protein peptide capable of replacing fish meal and preparation method and application of mushroom bran protein peptide in aquaculture |
| CN105941823A (en) * | 2016-05-25 | 2016-09-21 | 刘新旗 | High-soluble soybean peptide dry powder and preparation method thereof |
| CN108041404A (en) * | 2017-11-27 | 2018-05-18 | 余雪平 | Nutrient plant Gly-His-Lys and its production technology |
| CN109744532A (en) * | 2017-11-01 | 2019-05-14 | 北京神飞航天应用技术研究院 | A kind of suitable spacefarer adjusts the solid beverage and preparation method thereof of blood lipid and improvement memory |
| CN116746686A (en) * | 2023-06-20 | 2023-09-15 | 大连非得生物产业有限公司 | Tricholoma matsutake composite oligopeptide powder and preparation method thereof |
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| CN101691596A (en) * | 2009-10-15 | 2010-04-07 | 哈尔滨商业大学 | Methods of hydrolyzing soyabean protein to produce exorphins |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782768A (en) * | 2015-04-03 | 2015-07-22 | 傅水娟 | Functional drink food and preparation method thereof |
| CN105104756A (en) * | 2015-09-15 | 2015-12-02 | 江苏省苏微微生物研究有限公司 | Mushroom bran protein peptide capable of replacing fish meal and preparation method and application of mushroom bran protein peptide in aquaculture |
| CN105104756B (en) * | 2015-09-15 | 2018-08-07 | 江苏省苏微微生物研究有限公司 | A kind of mushroom bran protein peptides of alternative fish meal and preparation method thereof and the application in aquaculture |
| CN105941823A (en) * | 2016-05-25 | 2016-09-21 | 刘新旗 | High-soluble soybean peptide dry powder and preparation method thereof |
| CN109744532A (en) * | 2017-11-01 | 2019-05-14 | 北京神飞航天应用技术研究院 | A kind of suitable spacefarer adjusts the solid beverage and preparation method thereof of blood lipid and improvement memory |
| CN108041404A (en) * | 2017-11-27 | 2018-05-18 | 余雪平 | Nutrient plant Gly-His-Lys and its production technology |
| CN116746686A (en) * | 2023-06-20 | 2023-09-15 | 大连非得生物产业有限公司 | Tricholoma matsutake composite oligopeptide powder and preparation method thereof |
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