CN105248837A - Chlorella active polypeptide powder preparing method - Google Patents
Chlorella active polypeptide powder preparing method Download PDFInfo
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- CN105248837A CN105248837A CN201510782998.XA CN201510782998A CN105248837A CN 105248837 A CN105248837 A CN 105248837A CN 201510782998 A CN201510782998 A CN 201510782998A CN 105248837 A CN105248837 A CN 105248837A
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Abstract
The invention discloses a chlorella active polypeptide powder preparing method and relates to the chlorella deep processing and biotechnology fields. Nutritious chlorella powder prepared through combination of bacillus licheniformis fermentation and protein enzymatic hydrolysis is rich in polypeptide, technology is reasonable, rich nutrients are obtained, and the chlorella powder is fine, high in solubility and long in storage life. The chlorella polypeptide powder can be made into capsules, tablets and granules with the conventional method, can serve as health food, can make an organism generate antimicrobial active substances, kill pathogenic bacteria and improve the immunity of the human body, has remarkable effects in reducing blood pressure and blood fat, resisting tumor and radiation, preventing and controlling gastric ulcers and ulcerative colitis and the like, and has remarkable social meaning and economic meaning.
Description
Technical field
The present invention relates to chlorella deep processing and biological technical field, be specifically related to a kind of preparation method of chlorella active peptides powder.
Background technology
Chlorella is the general natural disposition monoplast green alga of a class, belongs to Chlorophyta, Chlorella, fast growth, is easy to cultivate, and using value is high.Chlorella contains abundant bioactivator and medicinal ingredient, contains great potential as a kind of novel healthy food and medicine.Chlorella has antitumor activity, increases immunity, removing toxic substances protects the liver, hypotensive effect etc., its crude protein content high (about 50%), quality better, has become that chlorella application is very active, an aspect of attention.Biologically active peptide refers to that those have the peptide class of special physiological activity or functional characteristic.Most protein are all the precursor substances of the biologically active peptide with certain function, also exist in its peptide chain structure and there is certain bioactive amino acid sequence segments (functional areas), in normal state, its functional areas peptide section is hidden in peptide chain, but once discharge from protein peptide chain separately, under suitable environment, just can demonstrate unique biologically active, this functional peptide fragment is exactly biologically active peptide.Modern biotechnology metabolism research finds: the protein of human consumption, after gastral multiple enzyme hydrolysis, is more directly absorb with the form of low peptide, has higher nutritive value and biological value.Enzyme hydrolysis method obtains the Main Means that biologically active peptide has become suitability for industrialized production biologically active peptide.
Summary of the invention
The object of this invention is to provide a kind of preparation method of chlorella active peptides powder, solve that chlorella crude protein utilization rate is low, taste bad will, have the problem of fishy smell.
To achieve these goals, the present invention is achieved by the following technical solutions:
A preparation method for chlorella active peptides powder, comprises the following steps:
(1) get the raw materials ready: the algae liquid containing live body chlorella adopts supercentrifugal process to isolate chlorella, by washed with de-ionized water, drain process algae mud and reach 45-55% to water content, for subsequent use;
(2) fermentable: bacillus licheniformis is inoculated in and expands on culture medium, the expansion carrying out 18-24h is cultivated, again the bacillus licheniformis of expansion is forwarded in the algae mud of step (1) gained, adjust ph is 6.5-8.5, be 25-40 DEG C in temperature, shaking speed is cultivate 6-10 days under 120-160rpm condition;
(3) enzymolysis: after fermentation ends, adds deionized water, and make solid-liquid ratio in zymotic fluid reach 1:10, adjusted to ph is 6.8-8.5, adds neutral proteinase 10-30U/g, lysozyme 10-20U/g, under temperature is 30-45 DEG C of condition, and enzymolysis 7-8h;
(4) spraying dry: enzymolysis liquid material spray is dry, and obtain polypeptide powder, drying process with atomizing parameter is: EAT 185-205 DEG C, leaving air temp 50-65 DEG C.
Above-mentioned steps (1) in, in described algae liquid, chlorella cells concentration is 250-500mg/L.
Above-mentioned steps (2) in, the proportioning of described expansion culture medium is corn steep liquor 10-15g, soy peptone 7-10g, glucose 15-25g, water 1L, pH7.0, sterilizing 15-20min under 0.1MPa condition.
Above-mentioned steps (2) in, the inoculum concentration of the bacillus licheniformis of expansion is the 8-10wt% of algae mud.
As preferably, above-mentioned steps (3) in, adjusted to ph is 7.0, adds neutral proteinase 20U/g, lysozyme 15U/g, under temperature is 37.5 DEG C of conditions, enzymolysis 7.5h;
As preferably, above-mentioned steps (4) in, drying process with atomizing parameter is: EAT 195 DEG C, leaving air temp 55 DEG C.
Beneficial effect of the present invention: the nutrition chlorella powder that the present invention utilizes bacillus licheniformis fermentation in combining protease method to be hydrolyzed preparation is rich in polypeptide, rational technology, nutritious, matter is thin, dissolubility is good, long shelf-life, this chlorella polypeptide powder can be prepared into capsule in conventional manner, tablet and granule, can be used as health food, body can be impelled to produce antibacterial substance, kill pathogenic bacteria, strengthen body immunity, hypotensive, reducing blood lipid, antitumor, radioresistance, prevent and treat the aspect such as gastric ulcer and ulcerative colitis and have remarkable result, there is significant social effect and economic implications.
Detailed description of the invention
The present invention is set forth further in conjunction with the embodiments, do not form and scope of the present invention is limited.
Embodiment 1
The algae liquid being 500mg/L live body chlorella containing cell concentration adopts supercentrifugal process to isolate chlorella, by washed with de-ionized water, drains process algae mud and reaches 55% to water content, for subsequent use.Corn steep liquor 15g, soy peptone 10g, glucose 25g, water 1L are mixed, regulate pH7.0, under 0.1MPa condition, sterilizing 20min obtains expanding culture medium, be inoculated in by bacillus licheniformis and expand on culture medium, the expansion carrying out 24h is cultivated, then is forwarded in the algae mud of gained by the bacillus licheniformis of expansion, inoculum concentration is 10wt%, adjust ph is 8.5, is 40 DEG C in temperature, and shaking speed is cultivate 10 days under 160rpm condition.After fermentation ends, add deionized water, make solid-liquid ratio in zymotic fluid reach 1:10, adjusted to ph is 8.5, adds neutral proteinase 30U/g, lysozyme 20U/g, under temperature is 45 DEG C of conditions, and enzymolysis 8h.Enzymolysis liquid material spray is dry, and obtain polypeptide powder, drying process with atomizing parameter is: EAT 205 DEG C, leaving air temp 65 DEG C.
Embodiment 2
The algae liquid being 250mg/L live body chlorella containing cell concentration adopts supercentrifugal process to isolate chlorella, by washed with de-ionized water, drains process algae mud and reaches 45% to water content, for subsequent use.Corn steep liquor 10g, soy peptone 7g, glucose 15g, water 1L are mixed, regulate pH7.0, under 0.1MPa condition, sterilizing 15min obtains expanding culture medium, be inoculated in by bacillus licheniformis and expand on culture medium, the expansion carrying out 18h is cultivated, then is forwarded in the algae mud of gained by the bacillus licheniformis of expansion, inoculum concentration is 8wt%, adjust ph is 6.5, is 25 DEG C in temperature, and shaking speed is cultivate 6 days under 120rpm condition.After fermentation ends, add deionized water, make solid-liquid ratio in zymotic fluid reach 1:10, adjusted to ph is 6.8, adds neutral proteinase 10U/g, lysozyme 10U/g, under temperature is 30 DEG C of conditions, and enzymolysis 7h.Enzymolysis liquid material spray is dry, and obtain polypeptide powder, drying process with atomizing parameter is: EAT 185 DEG C, leaving air temp 50 DEG C.
Embodiment 3
The algae liquid being 400mg/L live body chlorella containing cell concentration adopts supercentrifugal process to isolate chlorella, by washed with de-ionized water, drains process algae mud and reaches 50% to water content, for subsequent use.Corn steep liquor 12.5g, soy peptone 8.5g, glucose 20g, water 1L are mixed, regulate pH7.0, under 0.1MPa condition, sterilizing 17min obtains expanding culture medium, be inoculated in by bacillus licheniformis and expand on culture medium, the expansion carrying out 20h is cultivated, then is forwarded in the algae mud of gained by the bacillus licheniformis of expansion, inoculum concentration is 9wt%, adjust ph is 7.5, is 37.5 DEG C in temperature, and shaking speed is cultivate 8 days under 150rpm condition.After fermentation ends, add deionized water, make solid-liquid ratio in zymotic fluid reach 1:10, adjusted to ph is 7.0, adds neutral proteinase 20U/g, lysozyme 15U/g, under temperature is 37.5 DEG C of conditions, and enzymolysis 7.5h.Enzymolysis liquid material spray is dry, and obtain polypeptide powder, drying process with atomizing parameter is: EAT 195 DEG C, leaving air temp 55 DEG C.
Experimental example
The content of polypeptide in the polypeptide powder that detection embodiment 1-3 makes.
The detection method of conversion ratio: free amino acid adopts formol titration, protein adopts Kjeldahl's method, and polypeptide adopts biuret method to detect.
Polypeptide conversion ratio computing formula: conversion ratio=(T2-T1)/(P1-P2).In formula: T2 is content of peptides after enzymolysis; T1 is content of peptides before enzymolysis; P1 is total protein content before enzymolysis; P2 is acid-soluble protein content before enzymolysis.
Experimental result, following table record:
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Total protein content | >50％ | >50％ | >50％ |
| Water-soluble polypeptide | >15％ | >15％ | >15％ |
| Fat | <5％ | <5％ | <5％ |
| Inorganic salts | >1.5％ | >1.5％ | >1.5％ |
| Coliform count | < 30/100g | < 30/100g | < 30/100g |
Conclusion: from above-mentioned experimental result, in chlorella active peptides powder of the present invention, contained content of peptides is high, and conversion ratio is all higher, especially the chlorella active peptides powder made of embodiment 3, and the conversion ratio of polypeptide is up to 86.9%.
Claims (6)
1. a preparation method for chlorella active peptides powder, is characterized in that: comprise the following steps:
(1) get the raw materials ready: the algae liquid containing live body chlorella adopts supercentrifugal process to isolate chlorella, by washed with de-ionized water, drain process algae mud and reach 45-55% to water content, for subsequent use;
(2) fermentable: bacillus licheniformis is inoculated in and expands on culture medium, the expansion carrying out 18-24h is cultivated, again the bacillus licheniformis of expansion is forwarded in the algae mud of step (1) gained, adjust ph is 6.5-8.5, be 25-40 DEG C in temperature, shaking speed is cultivate 6-10 days under 120-160rpm condition;
(3) enzymolysis: after fermentation ends, adds deionized water, and make solid-liquid ratio in zymotic fluid reach 1:10, adjusted to ph is 6.8-8.5, adds neutral proteinase 10-30U/g, lysozyme 10-20U/g, under temperature is 30-45 DEG C of condition, and enzymolysis 7-8h;
(4) spraying dry: enzymolysis liquid material spray is dry, and obtain polypeptide powder, drying process with atomizing parameter is: EAT 185-205 DEG C, leaving air temp 50-65 DEG C.
2. the preparation method of a kind of chlorella active peptides powder according to claim 1, is characterized in that: above-mentioned steps (1) in, in described algae liquid, chlorella cells concentration is 250-500mg/L.
3. the preparation method of a kind of chlorella active peptides powder according to claim 1, it is characterized in that: above-mentioned steps (2) in, the proportioning of described expansion culture medium is corn steep liquor 10-15g, soy peptone 7-10g, glucose 15-25g, water 1L, pH7.0, sterilizing 15-20min under 0.1MPa condition.
4. the preparation method of a kind of chlorella active peptides powder according to claim 1, is characterized in that: above-mentioned steps (2) in, the inoculum concentration of the bacillus licheniformis of expansion is the 8-10wt% of algae mud.
5. the preparation method of a kind of chlorella active peptides powder according to claim 1, is characterized in that: above-mentioned steps (3) in, adjusted to ph is 7.0, adds neutral proteinase 20U/g, lysozyme 15U/g, under temperature is 37.5 DEG C of conditions, enzymolysis 7.5h.
6. the preparation method of a kind of chlorella active peptides powder according to claim 1, is characterized in that: above-mentioned steps (4) in, drying process with atomizing parameter is: EAT 195 DEG C, leaving air temp 55 DEG C.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701310A (en) * | 2016-12-05 | 2017-05-24 | 华南理工大学 | Comprehensive utilization method of green algae biomass resources |
| CN113106136A (en) * | 2021-05-21 | 2021-07-13 | 厦门元之道生物科技有限公司 | Chlorella polypeptide and preparation method thereof |
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| CN101348754A (en) * | 2007-07-16 | 2009-01-21 | 青岛啤酒股份有限公司 | Spirulina extract and preparation thereof |
| CN102613384A (en) * | 2012-04-18 | 2012-08-01 | 刘锦胜 | Method for preparing spiral seaweed polypeptide powder by using living spiral seaweeds |
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- 2015-11-16 CN CN201510782998.XA patent/CN105248837A/en active Pending
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| CN101348754A (en) * | 2007-07-16 | 2009-01-21 | 青岛啤酒股份有限公司 | Spirulina extract and preparation thereof |
| CN101265468A (en) * | 2008-05-16 | 2008-09-17 | 张明洞 | Method for preparing protein kinase, active peptide and proteinase from spirulina |
| CN102613384A (en) * | 2012-04-18 | 2012-08-01 | 刘锦胜 | Method for preparing spiral seaweed polypeptide powder by using living spiral seaweeds |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701310A (en) * | 2016-12-05 | 2017-05-24 | 华南理工大学 | Comprehensive utilization method of green algae biomass resources |
| CN113106136A (en) * | 2021-05-21 | 2021-07-13 | 厦门元之道生物科技有限公司 | Chlorella polypeptide and preparation method thereof |
| CN113106136B (en) * | 2021-05-21 | 2023-06-30 | 厦门元之道生物科技有限公司 | Chlorella polypeptide and preparation method thereof |
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Application publication date: 20160120 |