CN106086137A - A kind of method utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide - Google Patents
A kind of method utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide Download PDFInfo
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- CN106086137A CN106086137A CN201610626633.2A CN201610626633A CN106086137A CN 106086137 A CN106086137 A CN 106086137A CN 201610626633 A CN201610626633 A CN 201610626633A CN 106086137 A CN106086137 A CN 106086137A
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- penaeus seu
- seu panulirus
- crusta penaeus
- shell meal
- oxidation peptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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Abstract
The present invention provides a kind of method utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide, step is as follows: A) Crusta Penaeus seu Panulirus is cleaned remove impurity, dry 20～26 hours at 40～60 DEG C, use pulverizer pulverizing, cross 40～100 mesh sieves, make shrimp shell meal, then add water and be configured to the shrimp shell meal solution that mass percent is 4～8%；B) by the pH regulator of shrimp shell meal solution to 12.5～13, it is subsequently adding protease 1000～5000U/g, 20～80 DEG C of water-baths hydrolyze 1～4 hour, hydrolysis is carried out under stirring, and keep temperature and pH constant, obtaining Crusta Penaeus seu Panulirus protein hydrolyzate, the addition adding fermented by biological enzyme liquid is 50 60u/g；The present invention utilizes physics and biotechnology preparation method simple, and abundant raw material is easy to get, and Crusta Penaeus seu Panulirus used is the by-product of shrimps processing, with low cost, add the added value of shrimp product, and avoid environment, technique is the most ripe, and equipment is simple, and preparation time is short.
Description
Technical field
The present invention relates to Lobster utilizes field, a kind of side utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide
Method.
Background technology
Anti-oxidation peptide is one main kind of in antioxidant, generally having suppression biomacromolecule peroxidating or purged body
The biologically active peptide of interior freedom and function becomes anti-oxidation peptide.Anti-oxidation peptide is present in long chains of protein with inactive state,
Use suitable method aminosal can obtain these oligopeptide, and they have crude protein or composition aminoacid is not had
Some functions, have very close relationship with the Metabolism regulation of body, immunity, endocrine, aging etc., rise in vital movement
Very important effect.In the last few years, along with people's pay attention to day by day to synthetized oxidation preventive agent safety problem, natural anti-oxidation
Peptide, because its stronger non-oxidizability and high security, just receives more and more attention.Both at home and abroad Natural Antioxidant Peptides is entered
Having gone numerous studies, existing plurality kinds of health care product and medicine are introduced to the market, and market prospect is the most considerable.Anti-oxidation peptide is mainly by egg
White matter produces through protease hydrolysis, and the kinds of protein of employing is a lot, the most mainly land protein resource, including waiting rice
Albumen, zein, lactalbumin, casein etc..Additionally, extract albumen from marine organisms to prepare anti-oxidation peptide the most increasingly
Paid close attention to by people, such as, utilize eupolyphoge sinensis, scallop, sharkskin etc. to prepare anti-oxidation peptide.
Summary of the invention
In order to overcome the deficiency in background technology, the invention discloses a kind of method utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide.
The technical scheme is that a kind of method utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide, comprise the steps:
A) Crusta Penaeus seu Panulirus is cleaned remove impurity, dry 20～26 hours at 40～60 DEG C, use pulverizer pulverizing, cross 40～100 mesh
Sieve, makes shrimp shell meal, then adds water and be configured to the shrimp shell meal solution that mass percent is 4～8%；
B) by the pH regulator of shrimp shell meal solution to 12.5～13, it is subsequently adding protease 1000～5000U/g, at 20～80 DEG C of water
Hydrolyzing 1～4 hour in bath, hydrolysis is carried out under stirring, and keeps temperature and pH constant, obtains Crusta Penaeus seu Panulirus protein hydrolyzate,
The addition adding fermented by biological enzyme liquid is 50-60u/g；
C), by Crusta Penaeus seu Panulirus protein hydrolyzate in 85～100 DEG C of water-bath inactivated proteases 10～20 minutes, after being cooled to room temperature, add
Organic acid be mass concentration be the citric acid solution of 50%, described citric acid solution addition be material liquid volume than 50:1-60:
1；It is centrifuged 10～20 minutes under 2000～6000r/min rotating speeds, takes supernatant, with surpassing of molecular cut off≤0000Da
Filter membrane carries out ultrafiltration, obtains Crusta Penaeus seu Panulirus polypeptide ultrafiltrate；
D) carry out Crusta Penaeus seu Panulirus polypeptide ultrafiltrate being evaporated under reduced pressure concentrating and vacuum lyophilization, obtain Crusta Penaeus seu Panulirus anti-oxidation peptide product.
Preferably, in step D) in the temperature of vacuum lyophilization be-20 degree.
Beneficial effects of the present invention: the present invention utilizes physics and biotechnology preparation method simple, and abundant raw material is easy to get, institute
With the by-product that Crusta Penaeus seu Panulirus is shrimps processing, with low cost, add the added value of shrimp product, and avoid environment is caused
Polluting, technique is the most ripe, and equipment is simple, and preparation time is short, easy to operate, seriality good, produces efficiently, and product form is managed
Thinking, pollution-free, it is adaptable to industrialized production, raw material sources are extensively easy to get, and have saved the energy, it is simple to universal and popularization.
Detailed description of the invention
The embodiment provided the present invention in conjunction with specific embodiments is described in further detail:
A kind of method utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide of the present invention, comprises the steps:
A) Crusta Penaeus seu Panulirus is cleaned remove impurity, dry 20～26 hours at 40～60 DEG C, use pulverizer pulverizing, cross 40～100 mesh
Sieve, makes shrimp shell meal, then adds water and be configured to the shrimp shell meal solution that mass percent is 4～8%；
B) by the pH regulator of shrimp shell meal solution to 12.5～13, it is subsequently adding protease 1000～5000U/g, at 20～80 DEG C of water
Hydrolyzing 1～4 hour in bath, hydrolysis is carried out under stirring, and keeps temperature and pH constant, obtains Crusta Penaeus seu Panulirus protein hydrolyzate,
The addition adding fermented by biological enzyme liquid is 50-60u/g；
C), by Crusta Penaeus seu Panulirus protein hydrolyzate in 85～100 DEG C of water-bath inactivated proteases 10～20 minutes, after being cooled to room temperature, add
Organic acid be mass concentration be the citric acid solution of 50%, described citric acid solution addition be material liquid volume than 50:1-60:
1；It is centrifuged 10～20 minutes under 2000～6000r/min rotating speeds, takes supernatant, with surpassing of molecular cut off≤0000Da
Filter membrane carries out ultrafiltration, obtains Crusta Penaeus seu Panulirus polypeptide ultrafiltrate；
D) carry out Crusta Penaeus seu Panulirus polypeptide ultrafiltrate being evaporated under reduced pressure concentrating and vacuum lyophilization, obtain Crusta Penaeus seu Panulirus anti-oxidation peptide product.
Preferably, in step D) in the temperature of vacuum lyophilization be-20 degree.
In this description, the present invention is described with reference to its specific embodiment.But it is clear that still can make
Various modifications and alterations are without departing from the spirit and scope of the present invention.Therefore, description is considered as illustrative rather than limits
Property processed.
Claims (2)
1. one kind utilizes the method that Crusta Penaeus seu Panulirus prepares anti-oxidation peptide, it is characterised in that its step is as follows:
A) Crusta Penaeus seu Panulirus is cleaned remove impurity, dry 20～26 hours at 40～60 DEG C, use pulverizer pulverizing, cross 40～100 mesh
Sieve, makes shrimp shell meal, then adds water and be configured to the shrimp shell meal solution that mass percent is 4～8%；
B) by the pH regulator of shrimp shell meal solution to 12.5～13, it is subsequently adding protease 1000～5000U/g, at 20～80 DEG C of water
Hydrolyzing 1～4 hour in bath, hydrolysis is carried out under stirring, and keeps temperature and pH constant, obtains Crusta Penaeus seu Panulirus protein hydrolyzate,
The addition adding fermented by biological enzyme liquid is 50-60u/g；
C), by Crusta Penaeus seu Panulirus protein hydrolyzate in 85～100 DEG C of water-bath inactivated proteases 10～20 minutes, after being cooled to room temperature, add
Organic acid be mass concentration be the citric acid solution of 50%, described citric acid solution addition be material liquid volume than 50:1-60:
1；It is centrifuged 10～20 minutes under 2000～6000r/min rotating speeds, takes supernatant, with surpassing of molecular cut off≤0000Da
Filter membrane carries out ultrafiltration, obtains Crusta Penaeus seu Panulirus polypeptide ultrafiltrate；
D) carry out Crusta Penaeus seu Panulirus polypeptide ultrafiltrate being evaporated under reduced pressure concentrating and vacuum lyophilization, obtain Crusta Penaeus seu Panulirus anti-oxidation peptide product.
The method utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide the most according to claim 1, it is characterised in that in step D) in vacuum
Cryodesiccated temperature is-20 degree.
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| CN201610626633.2A CN106086137A (en) | 2016-08-03 | 2016-08-03 | A kind of method utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide |
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| CN201610626633.2A CN106086137A (en) | 2016-08-03 | 2016-08-03 | A kind of method utilizing Crusta Penaeus seu Panulirus to prepare anti-oxidation peptide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106880052A (en) * | 2016-12-30 | 2017-06-23 | 浙江海洋大学 | The nutrition fortifier prepared using shrimping beam trawl |
| CN106967772A (en) * | 2017-03-24 | 2017-07-21 | 中国水产科学研究院长江水产研究所 | A kind of method that utilization magnetic immobilized alkali protease extracts shrimp shell albumen |
| CN108130353A (en) * | 2017-11-22 | 2018-06-08 | 浙江海洋大学 | A kind of method that oligopeptides powder is prepared using krill shell |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676623A (en) * | 2012-05-23 | 2012-09-19 | 浙江大学 | Method for preparing antioxidant peptide from shrimp shell |
| CN105567772A (en) * | 2016-01-14 | 2016-05-11 | 华南理工大学 | High oxidation resistance protein peptide and preparation method and application thereof |
| CN105661226A (en) * | 2016-01-14 | 2016-06-15 | 华南理工大学 | Crab meat antioxidant peptide liquid and crab meat protein-peptide mixed drink |
-
2016
- 2016-08-03 CN CN201610626633.2A patent/CN106086137A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676623A (en) * | 2012-05-23 | 2012-09-19 | 浙江大学 | Method for preparing antioxidant peptide from shrimp shell |
| CN105567772A (en) * | 2016-01-14 | 2016-05-11 | 华南理工大学 | High oxidation resistance protein peptide and preparation method and application thereof |
| CN105661226A (en) * | 2016-01-14 | 2016-06-15 | 华南理工大学 | Crab meat antioxidant peptide liquid and crab meat protein-peptide mixed drink |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106880052A (en) * | 2016-12-30 | 2017-06-23 | 浙江海洋大学 | The nutrition fortifier prepared using shrimping beam trawl |
| CN106967772A (en) * | 2017-03-24 | 2017-07-21 | 中国水产科学研究院长江水产研究所 | A kind of method that utilization magnetic immobilized alkali protease extracts shrimp shell albumen |
| CN108130353A (en) * | 2017-11-22 | 2018-06-08 | 浙江海洋大学 | A kind of method that oligopeptides powder is prepared using krill shell |
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Application publication date: 20161109 |