CN105494922A - Method for preparing selenium-enriched yeast autolysis powder through two-enzyme method - Google Patents
Method for preparing selenium-enriched yeast autolysis powder through two-enzyme method Download PDFInfo
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- CN105494922A CN105494922A CN201510965673.5A CN201510965673A CN105494922A CN 105494922 A CN105494922 A CN 105494922A CN 201510965673 A CN201510965673 A CN 201510965673A CN 105494922 A CN105494922 A CN 105494922A
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Abstract
The invention relates to a method for preparing selenium-enriched yeast autolysis powder through a two-enzyme method. The yeast autolysis powder is rich in glutathione and organic selenium, and mother liquor obtained after glutathione is extracted is pretreated, mixed with fresh yeast, subjected to enzymolysis and dried to obtain the yeast autolysis powder. The method includes the concrete steps of pretreating glutathione extracting mother liquor, conducting mother liquor sedimentation, conducting homogeneous wall breaking, carrying out primary enzymolysis, carrying out secondary enzymolysis and carrying out spray drying after secondary enzymolysis is finished to obtain the selenium-enriched yeast autolysis powder. The method has the advantages that the mother liquor obtained after glutathione is extracted is recovered in a reasonable mode, waste is turned into wealth, and waste of glutathione and amino acid polypeptide in the mother liquor is avoided; obtained autolysis powder is high in content of total nitrogen and amino acid nitrogen, high in nutritive value, low in ash content and good in product quality.
Description
Technical field
The present invention relates to a kind of preparation method of yeast derivative, relate to the method that two enzymes method prepares Se-enriched yeast self-dissolving powder specifically.
Background technology
Yeast is widely used in the field such as bread fermentation, beer fermentation, yeast of a great variety, comprises Saccharomyces cerevisiae, brewer's yeast, bakers' yeast, containing the material such as rich in protein, amino acid in yeast, by to yeast hydrolysis, the materials such as the protein of yeast or amino acid can be obtained.
Autolysing yeast powder selects special yeast cake to be raw material, adopt the new and high technology such as high-efficiency wall breaking and multi-joint enzymolysis, form through purification, product is rich in nucleotides, the multiple nutritional components such as the little peptide of nutrition, glutathione and yeast cell wall polysaccharide, not containing carrier, high temperature resistant, can granulate, to animal, there is splendid attractant, immunity and growth promotion.Be applicable to aquatic products, livestock and poultry and feed for pet.
Rich glutathione yeast is for the acquisition glutathione that ferments, the extracting method that traditional cost is minimum is mantoquita method, yeast extract containing GSH and cuprous oxide react to generate and precipitate GSCu, then reduction obtains GSH, but remove in the mother liquor after precipitation still containing a large amount of glutathione and amino acid, polypeptide, and in prior art, mother liquor is mostly discharged into treatment tank and does wastewater treatment, does not reclaim comprehensive utilization.
Summary of the invention
The object of the invention is to solve existing deficiency, provide two enzymes method to prepare the method for Se-enriched yeast self-dissolving powder, glutathione is extracted mother liquor turns waste into wealth, and obtains the Se-enriched yeast powder that glutathione content is high, be of high nutritive value.
The technical solution adopted for the present invention to solve the technical problems is:
Two enzymes method prepares the method for Se-enriched yeast self-dissolving powder, and described Se-enriched yeast self-dissolving powder is rich in Organic Selenium and glutathione, and the preparation method of described Se-enriched yeast self-dissolving powder carries out as follows:
(1) glutathione extracts mother liquor pretreatment: add vulcanized sodium by 0.5-0.8Kg/ ton mother liquor, in stirring at normal temperature reaction 10-15min, then mother liquor pH to 5.6-5.8 is adjusted with barium hydroxide, stir 30-45min, ensure that barium hydroxide and mother liquor fully react, the pH value that described glutathione extracts mother liquor is 1.8-2.5, glutathione content 0.5-0.6g/L, and solid quality content is 5-5.5%;
(2) mother liquor sedimentation: the completely reacted mother liquor of step (1) delivers to more than subsider natural subsidence 20-24h, then supernatant or suction filtration or plate compression, fully remove impurity stand-by;
(3) collect the Se-enriched yeast mud of fresh water capacity 40-50%, add the mother liquor of step (2), be warming up to 55-60 DEG C, stir, then adopt high pressure homogenizer homogeneous 1-2 time;
(4) primary enzymolysis, yeast juice NaOH after high-pressure homogeneous adjusts pH to 7.20-7.5, then adjusting temperature is 50-55 DEG C, the alkali protease that by volume mark 10-12 ‰ adds 10-20 ten thousand unit carries out primary enzymolysis, stir rear beginning timing enzymolysis 5-8 hour, process temperature maintains 50-55 DEG C, pH allows it naturally decline, and when pH to 6.0-6.1 left and right, primary enzymolysis terminates;
(5) secondary enzymolysis: after primary enzymolysis, adjust pH to 5.4, temperature 50-55 DEG C with dilute sulfuric acid, then by volume mark 0.8-1 ‰ adds the bromelain of 80-100 ten thousand unit, and enzymolysis 0.5-1.5 hour, process pH allow it naturally decline;
(6) dry: after secondary enzymolysis terminates, to be warming up to 80-82 DEG C, maintain 5-10min inactivator, then spray-dried acquisition rich glutathione nucleic acid yeast powder.
In step (1), vulcanized sodium is existing uses water-soluble solution, and the mode then added with stream adds in mother liquor to be pre-treated, and limit stream edged stirs.
In step (3), the mass ratio of mother liquor and yeast paste is 1:1, and the organic selenium content of Se-enriched yeast counts 0.15-0.2% with butt.
The invention has the beneficial effects as follows: (1) is reclaimed by reasonable manner and extracted the mother liquor after glutathione, turns waste into wealth, avoid the waste of glutathione in mother liquor and amino acid polypeptide composition, reduce cost of sewage disposal; (2) adopt rational enzymolysis process, the Se-enriched yeast powder total nitrogen of acquisition, amino acid nitrogen content are high, and be of high nutritive value, ash content is low, superior product quality.
Detailed description of the invention
Below by specific embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1:
Two enzymes method prepares the method for Se-enriched yeast self-dissolving powder, and described Se-enriched yeast self-dissolving powder is rich in Organic Selenium and glutathione, and the preparation method of described Se-enriched yeast self-dissolving powder carries out as follows:
(1) glutathione extracts mother liquor pretreatment: add vulcanized sodium by 0.5Kg/ ton mother liquor, in stirring at normal temperature reaction 10min, then mother liquor pH to 5.6 is adjusted with barium hydroxide, stir 30min, ensure that barium hydroxide and mother liquor fully react, the pH value that described glutathione extracts mother liquor is 1.8, glutathione content 0.5g/L, and solid quality content is 5%;
(2) mother liquor sedimentation: the completely reacted mother liquor of step (1) delivers to more than subsider natural subsidence 20h, then supernatant or suction filtration or plate compression, fully remove impurity stand-by;
(3) collect the Se-enriched yeast mud of fresh water capacity 40%, add the mother liquor of step (2), be warming up to 55 DEG C, stir, then adopt high pressure homogenizer homogeneous 1 time;
(4) primary enzymolysis, yeast juice NaOH after high-pressure homogeneous adjusts pH to 7.20, then adjusting temperature is 50 DEG C, the alkali protease of by volume mark 10 ‰ interpolation 200,000 units carries out primary enzymolysis, stir rear beginning timing enzymolysis 5 hours, process temperature maintains 50 DEG C, and pH allows it naturally decline, when pH to about 6.0 time primary enzymolysis terminate;
(5) secondary enzymolysis: after primary enzymolysis, adjust pH to 5.4 with dilute sulfuric acid, temperature 50 C, then by volume mark 0.8 ‰ adds the bromelain of 1,000,000 units, enzymolysis 0.5 hour, and process pH allows it naturally decline;
(6) dry: after secondary enzymolysis terminates, to be warming up to 80 DEG C, maintain 5min inactivator, then spray-dried acquisition rich glutathione nucleic acid yeast powder.
In step (1), vulcanized sodium is existing uses water-soluble solution, and the mode then added with stream adds in mother liquor to be pre-treated, and limit stream edged stirs.
In step (3), the mass ratio of mother liquor and yeast paste is 1:1, and the organic selenium content of Se-enriched yeast counts 0.15% with butt.
Embodiment 2:
Two enzymes method prepares the method for Se-enriched yeast self-dissolving powder, and described Se-enriched yeast self-dissolving powder is rich in Organic Selenium and glutathione, and the preparation method of described Se-enriched yeast self-dissolving powder carries out as follows:
(1) glutathione extracts mother liquor pretreatment: add vulcanized sodium by 0.8Kg/ ton mother liquor, in stirring at normal temperature reaction 15min, then mother liquor pH to 5.8 is adjusted with barium hydroxide, stir 45min, ensure that barium hydroxide and mother liquor fully react, the pH value that described glutathione extracts mother liquor is 2.5, glutathione content 0.6g/L, and solid quality content is 5.5%;
(2) mother liquor sedimentation: the completely reacted mother liquor of step (1) delivers to more than subsider natural subsidence 24h, then supernatant or suction filtration or plate compression, fully remove impurity stand-by;
(3) collect the Se-enriched yeast mud of fresh water capacity 50%, add the mother liquor of step (2), be warming up to 60 DEG C, stir, then adopt high pressure homogenizer homogeneous 2 times;
(4) primary enzymolysis, yeast juice NaOH after high-pressure homogeneous adjusts pH to 7.5, then adjusting temperature is 55 DEG C, the alkali protease of by volume mark 12 ‰ interpolation 200,000 units carries out primary enzymolysis, stir rear beginning timing enzymolysis 8 hours, process temperature maintains 55 DEG C, and pH allows it naturally decline, when pH to about 6.1 time primary enzymolysis terminate;
(5) secondary enzymolysis: after primary enzymolysis, adjust pH to 5.4 with dilute sulfuric acid, temperature 55 DEG C, then by volume mark 1 ‰ adds the bromelain of 800,000 units, enzymolysis 1.5 hours, and process pH allows it naturally decline;
(6) dry: after secondary enzymolysis terminates, to be warming up to 82 DEG C, maintain 10min inactivator, then spray-dried acquisition rich glutathione nucleic acid yeast powder.
In step (1), vulcanized sodium is existing uses water-soluble solution, and the mode then added with stream adds in mother liquor to be pre-treated, and limit stream edged stirs.
In step (3), the mass ratio of mother liquor and yeast paste is 1:1, and the organic selenium content of Se-enriched yeast counts 0.2% with butt.
In step (3), the mass ratio of mother liquor and yeast paste is 1:1.
The quality index of the selenium yeast self-dissolving powder that embodiment 1-2 prepares is as follows:
| Embodiment | Glutathione content | Total nitrogen content | Se content | Amino acid nitrogen content | Ash content |
| 1 | 0.685% | 12.8% | 0.12% | 4.2% | 0.98% |
| 2 | 0.82% | 13.5% | 0.18% | 4.5% | 1.0% |
Above-described embodiment is one of the present invention preferably scheme, not does any pro forma restriction to the present invention, also has other variant and remodeling under the prerequisite not exceeding the technical scheme described in claim.
Claims (3)
1. two enzymes method prepares the method for Se-enriched yeast self-dissolving powder, it is characterized in that: described Se-enriched yeast self-dissolving powder is rich in Organic Selenium and glutathione, and the preparation method of described Se-enriched yeast self-dissolving powder carries out as follows:
(1) glutathione extracts mother liquor pretreatment: add vulcanized sodium by 0.5-0.8Kg/ ton mother liquor, in stirring at normal temperature reaction 10-15min, then mother liquor pH to 5.6-5.8 is adjusted with barium hydroxide, stir 30-45min, ensure that barium hydroxide and mother liquor fully react, the pH value that described glutathione extracts mother liquor is 1.8-2.5, glutathione content 0.5-0.6g/L, and solid quality content is 5-5.5%;
(2) mother liquor sedimentation: the completely reacted mother liquor of step (1) delivers to more than subsider natural subsidence 20-24h, then supernatant or suction filtration or plate compression, fully remove impurity stand-by;
(3) collect the Se-enriched yeast mud of fresh water capacity 40-50%, add the mother liquor of step (2), be warming up to 55-60 DEG C, stir, then adopt high pressure homogenizer homogeneous 1-2 time;
(4) primary enzymolysis, yeast juice NaOH after high-pressure homogeneous adjusts pH to 7.20-7.5, then adjusting temperature is 50-55 DEG C, the alkali protease that by volume mark 10-12 ‰ adds 10-20 ten thousand unit carries out primary enzymolysis, stir rear beginning timing enzymolysis 5-8 hour, process temperature maintains 50-55 DEG C, pH allows it naturally decline, and when pH to 6.0-6.1 left and right, primary enzymolysis terminates;
(5) secondary enzymolysis: after primary enzymolysis, adjust pH to 5.4, temperature 50-55 DEG C with dilute sulfuric acid, then by volume mark 0.8-1 ‰ adds the bromelain of 80-100 ten thousand unit, and enzymolysis 0.5-1.5 hour, process pH allow it naturally decline;
(6) dry: after secondary enzymolysis terminates, to be warming up to 80-82 DEG C, maintain 5-10min inactivator, then spray-dried acquisition rich glutathione nucleic acid yeast powder.
2. the preparation method of rich glutathione nucleic acid yeast powder according to claim 1, is characterized in that: in step (1), vulcanized sodium is existing uses water-soluble solution, and the mode then added with stream adds in mother liquor to be pre-treated, and limit stream edged stirs.
3. the preparation method of rich glutathione nucleic acid yeast powder according to claim 1, is characterized in that: in step (3), the mass ratio of mother liquor and yeast paste is 1:1, and the organic selenium content of Se-enriched yeast counts 0.15-0.2% with butt.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755228A (en) * | 2016-11-21 | 2017-05-31 | 安徽省华信生物药业股份有限公司 | The technique for preparing the oligomeric Gly-His-Lys of selenium yeast using Se-enriched yeast |
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| CN101756151A (en) * | 2008-12-24 | 2010-06-30 | 安琪酵母股份有限公司 | Yeast extract with high glutamic acid content and preparation method thereof |
| CN101536772B (en) * | 2008-03-17 | 2011-04-20 | 济宁圣齐生物工程有限责任公司 | Large-scale industrialized technology for beer yeast extract |
| CN102293356A (en) * | 2011-09-21 | 2011-12-28 | 许文昌 | Yeast autolysate and preparation method thereof |
| CN102550802A (en) * | 2011-12-19 | 2012-07-11 | 成都宏安生物科技有限公司 | Method for extracting polypeptide and amino acid from waste beer yeast |
| CN104489601A (en) * | 2014-12-19 | 2015-04-08 | 山东圣琪生物有限公司 | Production method of beer yeast extract |
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- 2015-12-22 CN CN201510965673.5A patent/CN105494922A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101536772B (en) * | 2008-03-17 | 2011-04-20 | 济宁圣齐生物工程有限责任公司 | Large-scale industrialized technology for beer yeast extract |
| CN101756151A (en) * | 2008-12-24 | 2010-06-30 | 安琪酵母股份有限公司 | Yeast extract with high glutamic acid content and preparation method thereof |
| CN102293356A (en) * | 2011-09-21 | 2011-12-28 | 许文昌 | Yeast autolysate and preparation method thereof |
| CN102550802A (en) * | 2011-12-19 | 2012-07-11 | 成都宏安生物科技有限公司 | Method for extracting polypeptide and amino acid from waste beer yeast |
| CN104489601A (en) * | 2014-12-19 | 2015-04-08 | 山东圣琪生物有限公司 | Production method of beer yeast extract |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755228A (en) * | 2016-11-21 | 2017-05-31 | 安徽省华信生物药业股份有限公司 | The technique for preparing the oligomeric Gly-His-Lys of selenium yeast using Se-enriched yeast |
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