CN101536772B - Large-scale industrialized technology for beer yeast extract - Google Patents
Large-scale industrialized technology for beer yeast extract Download PDFInfo
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- CN101536772B CN101536772B CN2008100864588A CN200810086458A CN101536772B CN 101536772 B CN101536772 B CN 101536772B CN 2008100864588 A CN2008100864588 A CN 2008100864588A CN 200810086458 A CN200810086458 A CN 200810086458A CN 101536772 B CN101536772 B CN 101536772B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses new large-scale industrialized technology for producing novel yeast extract series products 3,000 t/a by using beer yeast slurry as an initial raw material, and belongs to the technical field of preparing series biological products from yeast. The production steps comprise preliminary treatment, debitterizing process, controllable hydrolysis process, separation and refining, condensation or spray drying. The technology is characterized in that the waste beer yeast with low cost is used as the raw material, the technology combining a physical wall-breaking method, an automatic dissolution method and an enzymolysis method is provided, the hydrolysis ratio of beer yeast protein reaches 55 percent, and the total yield reaches 60 percent. The invention also discloses a biochemical controllable debitterizing process, which solves the technical bottlenecking problems of more impurities, heavy bitter taste and low yield of the yeast slurry raw material. Three series and more than 20 yeast extract series products are successfully obtained. The comprehensive utilization rate of the yeast slurry is over 90 percent; and the technology is typical environment-friendly, clean and resource-saving technology and has remarkable economic and social benefits.
Description
Technical field:
The present invention relates to utilize beer waste yeast to produce the technical field of novel yeast extract series of products, relate in particular to the integrated approach that utilizes beer yeast slurry production food-grade simultaneously and technical grade yeast extract.
Background technology:
Before the present invention, yeast extract has been converted into productivity for the scientific and technological achievement of country's 95 tackling of key scientific and technical problems emphasis special topics, some large-scale yeast extract production firms are raw material with Saccharomyces cerevisiae (Bread yeast) and active dry yeast (active dry yeast) tail powder all, production food-grade yeast extract, be sold inside the country and outlet, but common problem is that expensive power consumption is big, and faces the challenge of environmental protection and resource.
In recent years, though some are arranged is the report that raw material extracts nucleic acid merely or produces flavoring or dusty yeast merely with beer waste yeast (mud), as the open day 2003.07.23 of a kind of method Chinese patent CN1431293 that utilizes beer waste yeast to make dusty yeast of State Oceanic Administration Bureau The Third Oceanography Institute; Hao Quanyuan extracts the open day 2002.09.25 of method Chinese patent CN1370837 of nucleotides from beer waste yeast; The open day 1991.06.19 of model essay mountain method for preparing flavouring liquid from beer waste yeast Chinese patent CN1052248.But their weak point, the one, product is compared with external product, and quality is not high; Secondly product is single, and this genus of the underflow waste water after extracting nucleic acid or preparing dusty yeast is lower as discharging of waste liquid or comprehensive utilization ratio; Its three extensive industrialized technology is incomplete, and does not have the production technology practice of 3000t/a beer yeast extract.
Summary of the invention:
One of purpose of the present invention is to adopt the leftover bits and pieces beer waste yeast (Waste beer yeast) of brewery, turn waste into wealth, research and development and production yeast extract, through research and development in 5 years and industrialization exploration, difficult problems such as successfully having solved yeast paste (yeast slurry) raw material impurity is many, heavy bitter taste, yield are low.
Another object of the present invention is to adopt combine impurity elimination, debitterize etc. of physical separation and Chemical Pretreatment to separate purification process; Remove alpha-acid, iso-and bitter substances such as polyphenol substance tannin, anthocyanin that brewer's yeast adsorbed hop resin etc. in Beer Brewage carries, make it to become the positive yeast of pure flavor, with regulating and control and optimizing the biochemical hydrolysising experiment technology of Yeast protein and reached product debitterize and the requirement of adaptation flavour of food products in conjunction with the food science and technology method.
Still a further object of the present invention is researched and developed, is produced novel yeast extract, breaks through each technical bottleneck, establishes the technical foundation of large-scale production, and becomes the combination point of flavouring science and technology, produces series of products.
The present invention compared with prior art has the following advantages:
1, renewable resource utilization innovation with maximize: the employed raw material of country's 95 scientific and technological public relations projects " yeast extraction " is the Saccharomyces cerevisiae (Bread yeast) and the active dry yeast (Active dry yeast) of food-grade, quality better but production cost height.The present invention adopts discarded yeast (mud) the yeast flavor agent " yeast extraction " identical for feedstock production goes out of aging after the 4th generation that beer fermentation produces, the exploitation and the application of all succeeding at field of food, in the industrial microorganism field, in feed additive field.
2, the biochemistry of Yeast protein hydrolysate bitter peptides component regulation and control and " debitterize " technology thereof with the embedding of the control of protease screening, degree of hydrolysis and natural high molecular substance, shelter, technical measures " debitterize " preferably, " national conditions that not too are fit to China with discarded beer waste yeast as the raw material of beer yeast extract " have been broken, judgements such as " difficult quality are stable ".
3, the innovation of finished product dry technology changes protein-colloid viscosity rerum natura principle big, that easily lump with cold airflow, transform conventional spray-drying---abrasive dust and the three-step combined technology of sieving, become the advanced drying process of step spray dry forming, " pollution " and irradiation sterilization have been prevented, improved process efficiency and product quality greatly, this technology is domesticly to create first of the finished product manufacturing process with kind is dry.
4, the innovation of comprehensive utilization technique: successfully be developed to the three major types product, and its remaining this genus of underflow waste water deep analysis and research have been done, find that it contains that crude protein 23% (giving money as a gift) and 18 seed amino acids are complete to be developed to two kinds of industrial products again, make the utilization rate of beer accessory substance yeast paste surpass 90%, accomplished reduction of discharging, environmental protection and economized on resources, significant.
Further set forth the present invention below by detailed description, but embodiment not a limitation of the present invention to the specific embodiment of the invention.
Embodiment 1
1. beer waste yeast washing: in reactor, add 1000g water, 18g NaHCO
3, stirred 5 minutes, add waste yeast 500g, in 10 ℃ of water, stirred 5 minutes, it is standby to put into the drier back yeast paste that removes slag
2. debitterize: the amount of debitterize agent is 1%, and the time is 60 minutes, and debitterize gets 8% washing yeast milk
3. broken wall: add 8% yeast paste 500g in reactor, high pressure homogenizer is homogeneous twice under 60MPa, makes breaking-wall cell.
4. hold certainly: after the yeast paste 200g behind the homogeneous broken wall is added 2 times of volume sterilized waters and 5%NaCl, stir, with 4mol/LNaOH pH is transferred to 8, add mass fraction again and be 1% nuclease, temperature is to 55 ℃ of self-dissolving 24h
5. enzymolysis and sterilization: will be through from holding the back yeast paste, with faintly acid protease 1# hydrolysis 24h under the condition of 60 ℃ of pH6.4, temperature, carry out Pasteur's high-temperature short-time sterilization then.
6. centrifugation: the yeast milk after the sterilization of step 5 enzymolysis is carried out high speed centrifugation twice, obtain the polysaccharide and the supernatant of solid content.
7. the solid content that obtains in the step 6 is carried out spray-drying and obtain the powdery polysaccharide.
8. the concentrate drying of supernatant: step 6 is separated the supernatant that obtains, after concentrating by vacuum and low temperature, enter spray-drying, obtain the dusty yeast extract.
Embodiment 2
1. beer waste yeast washing: in reactor, add 1000g water, 18g NaHCO
3, stirred 5 minutes, add waste yeast 500g, in 10 ℃ of water, stirred 5 minutes, it is standby to put into the drier back yeast paste that removes slag.
2. debitterize: the amount of debitterize agent is 1%, and the time is 60 minutes, and debitterize gets 8% washing yeast milk
3. broken wall: add 8% yeast paste 500g in reactor, high pressure homogenizer is homogeneous twice under 60MPa, makes breaking-wall cell.
4. hold certainly: after the yeast paste 200g behind the homogeneous broken wall is added 2 times of volume sterilized waters and 5%NaCl, stir, with 4mol/LNaOH pH is transferred to 8, add mass fraction again and be 1% nuclease, temperature is to 55 ℃ of self-dissolving 24h
5. enzymolysis and sterilization: will be through from holding the back yeast paste, with faintly acid protease 2# hydrolysis 24h under the condition of 60 ℃ of pH6.4, temperature, carry out Pasteur's high-temperature short-time sterilization then.
6. centrifugation: the yeast milk after the sterilization of step 5 enzymolysis is carried out high speed centrifugation twice, obtain the polysaccharide and the supernatant of solid content.
7. the solid content that obtains in the step 6 is carried out spray-drying and obtain the powdery polysaccharide.
8. the concentrate drying of supernatant: step 6 is separated the supernatant that obtains, concentrate by vacuum and low temperature, allotment obtains food-grade cream yeast extract repeatedly.
Embodiment 3
1. beer waste yeast washing: in reactor, add 1000g water, 18g NaHCO
3, stirred 5 minutes, add waste yeast 500g, in 10 ℃ of water, stirred 5 minutes, it is standby to put into the drier back yeast paste that removes slag.
2. debitterize: the amount of debitterize agent is 1%, and the time is 60 minutes, and debitterize gets 8% washing yeast milk.
3. broken wall: add 8% yeast milk 500g in reactor, high pressure homogenizer is homogeneous twice under 60MPa, makes breaking-wall cell.
4. self-dissolving: after the yeast paste 200g behind the homogeneous broken wall added 2 times of volume sterilized waters and 5%NaCl, stir, with 4mol/LNaOH pH is transferred to 8, add mass fraction again and be 1% nuclease, temperature is to 55 ℃ of self-dissolving 24h.
5. enzymolysis and sterilization: will be through from holding the back yeast paste, with faintly acid protease 3 # hydrolysis 24h under the condition of 60 ℃ of pH6.4, temperature, carry out Pasteur's high-temperature short-time sterilization then.
6. centrifugation: the yeast milk after the sterilization of step 5 enzymolysis is carried out high speed centrifugation twice, obtain the polysaccharide and the supernatant of solid content.
7. the solid content that obtains in the step 6 is carried out spray-drying and obtain the powdery polysaccharide.
8. the concentrate drying of supernatant: step 6 is separated the supernatant that obtains, composite after concentrating by vacuum and low temperature through reaction, enter spray-drying and obtain powdered food level yeast extract.
Embodiment 4
1. beer waste yeast washing: in reactor, add 1000g water, 18g NaHCO
3, stirred 5 minutes, add waste yeast 500g, in 10 ℃ of water, stirred 5 minutes, put into drier and remove slag the back yeast paste fully.
2. debitterize: the amount of debitterize agent is 1%, and the time is 60 minutes, and debitterize gets 8% washing yeast milk.
3. broken wall: add 8% yeast paste 500g in reactor, high pressure homogenizer is homogeneous twice under 60MPa, makes breaking-wall cell.
4. hold certainly: after the yeast paste 200g behind the homogeneous broken wall is added 2 times of volume sterilized waters and 5%NaCl, stir, with 4mol/LNaOH pH is transferred to 8, add mass fraction again and be 1% nuclease, temperature is to 55 ℃ of self-dissolving 24h.
5. enzymolysis and sterilization: will be through from holding the back yeast paste, with faintly acid protease 1# hydrolysis 24h under the condition of 60 ℃ of pH6.4, temperature, carry out Pasteur's high-temperature short-time sterilization then.
6. centrifugation: the yeast milk after the sterilization of step 5 enzymolysis is carried out high speed centrifugation twice, obtain the polysaccharide and the supernatant of solid content.
7. the solid content that obtains in the step 6 is carried out spray-drying and obtain the powdery polysaccharide.
8. the concentrate drying of supernatant: step 6 is separated the supernatant that obtains, after concentrating by vacuum and low temperature, enter spray-drying, obtain the dusty yeast extract.
Embodiment 5
1. beer waste yeast washing: in reactor, add 1000g water, 18gNaHCO
3, stirred 5 minutes, add waste yeast 500g, in 10 ℃ of water, stirred 5 minutes, it is standby to put into the drier back yeast paste that removes slag.
2. debitterize: the amount of debitterize agent is 1%, and the time is 60 minutes, and debitterize gets 8% washing yeast milk.
3. broken wall: add 8% yeast paste 500g in reactor, high pressure homogenizer is homogeneous twice under 60MPa, makes breaking-wall cell.
4. hold certainly: after the yeast paste 200g behind the homogeneous broken wall is added 2 times of volume sterilized waters and 5%NaCl, stir, with 4mol/LNaOH pH is transferred to 8, add mass fraction again and be 1% nuclease, temperature is to 55 ℃ of self-dissolving 24h.
5. enzymolysis and sterilization: will be through from holding the back yeast paste, with faintly acid protease 1# hydrolysis 24h under the condition of 60 ℃ of pH6.4, temperature, carry out Pasteur's high-temperature short-time sterilization then.
6. centrifugation: the yeast milk after the sterilization of step 5 enzymolysis is carried out high speed centrifugation twice, obtain the polysaccharide and the supernatant of solid content.
7. the solid content that obtains in the step 6 is carried out spray-drying and obtain the powdery polysaccharide.
8. the concentrate drying of supernatant: step 6 is separated the supernatant that obtains, after concentrating by vacuum and low temperature, enter spray-drying, obtain the dusty yeast extract.
Embodiment 6:
(1) yeast paste pretreatment of raw material, the removal of impurity, and add debitterize agent debitterize.
(2) self-dissolving enzymolysis.By the endobacillary endogenous enzymes of yeast,, and add enzyme preparation in the self-dissolving later stage and carry out directionally hydrolyzing soluble amino acid of the endobacillary proteolysis of yeast and polypeptide class.
(3) centrifugation.Yeast milk behind the self-dissolving enzymolysis is carried out high speed centrifugation, remove insoluble matters such as cell membrane.
(4) concentrate drying.Supernatant with after separating is concentrated to by vacuum and low temperature, enters spray-drying, obtains the dusty yeast extract.
(5) instant noodles chicken soup material package formulation material: salt, sugar, monosodium glutamate, I+G, the smart powder of chicken, chicken essence, ginger powder, onion powder, pepper powder, garlic powder, green onion powder, anticaking agent.With using traditional chicken soup stock bag prescription, in prescription, add 2%, 3%, 4%, 5% dusty yeast extract SXF-806 (formula table is as follows) respectively, according to flavoring local flavor sense of food key element, respectively 5 parts of test specimens are estimated.
Experiment showed, same prescription, after adding 0.8% ~ 5% yeast extract, the delicate flavour of the fragrant and sweet and plant protein hydrolysate of tunable meat extract produces sweet dense abundanter flavour, makes the satisfactory careful cunning of total sense of taste, and is more aromatic, richer variation.
Claims (3)
1. technology of utilizing beer waste yeast to produce yeast extract comprises following steps:
(1) after the beer waste yeast washing, adds debitterize agent debitterize;
(2) homogeneous broken wall: high pressure homogenizer is homogeneous twice under 60MPa, makes breaking-wall cell;
(3) self-dissolving: after the yeast paste behind the homogeneous broken wall added 2 times of volume sterilized waters and 5%NaCl, stir, pH is transferred to 8, add mass fraction again and be 1% nuclease, temperature adjustment to 55 ℃ self-dissolving 24h with 4mol/LNaOH;
(4) enzymolysis sterilization: the yeast milk after the self-dissolving with faintly acid protease hydrolysis 24h under the condition of 60 ℃ of pH6.4, temperature, is carried out Pasteur's high-temperature short-time sterilization then;
(5) centrifugation, drying: the yeast milk behind the enzymolysis is carried out high speed centrifugation, get supernatant, after concentrating by vacuum and low temperature, spray-drying obtains the dusty yeast extract.
2. according to the described technology of claim 1, it is characterized in that described washing is to add 1000g water, 18g NaHCO in reactor
3, stirred 5 minutes, add beer waste yeast 500g, in 10 ℃ of water, stirred 5 minutes, put into drier and remove slag.
3. according to the described technology of claim 1, it is characterized in that described debitterize agent consumption is 1% of the beer waste yeast after washing, the debitterize time is 60 minutes.
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| CN105494922A (en) * | 2015-12-22 | 2016-04-20 | 虞涵晰 | Method for preparing selenium-enriched yeast autolysis powder through two-enzyme method |
| CN105581323A (en) * | 2015-12-22 | 2016-05-18 | 温州任和教育科技有限责任公司 | Preparation method of yeast powder high in nutritive value |
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| CN101974381B (en) * | 2010-10-21 | 2013-10-30 | 河北美邦工程科技有限公司 | Technology for recycling beer from beer waste yeast paste |
| CN102465165B (en) * | 2010-11-09 | 2014-09-03 | 安琪酵母股份有限公司 | Preparation method of bioactive peptide |
| CN102461894A (en) * | 2010-11-12 | 2012-05-23 | 天津市食品研究所有限公司 | Integrated technological method for producing yeast nutrient |
| CN102907669B (en) * | 2011-08-04 | 2014-12-31 | 上海杰康诺生物科技有限公司 | Production method for edible beer yeast powder |
| CN102488090B (en) * | 2011-11-28 | 2013-02-13 | 浙江大学 | High protein feed prepared by using waste yeast mud and method thereof |
| CN102550802B (en) * | 2011-12-19 | 2013-09-18 | 成都宏安生物科技有限公司 | Method for extracting polypeptide and amino acid from waste beer yeast |
| CN102925308B (en) * | 2012-10-31 | 2014-04-02 | 华南理工大学 | Method for preparing beer yeast paste by carboxymethyl chitosan debitterizing |
| CN104161259B (en) * | 2014-08-08 | 2016-02-10 | 广西湘桂生物科技有限公司 | A kind of breaking yeast cellule membrane also prepares the method for yeast extract |
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| CN115474681A (en) * | 2022-10-12 | 2022-12-16 | 湛江五洲生物工程有限公司 | Preparation process of yeast extract |
| CN115595221B (en) * | 2022-11-15 | 2024-01-19 | 青岛啤酒股份有限公司 | Method for removing hops bitter substances in beer yeast paste |
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Cited By (2)
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|---|---|---|---|---|
| CN105494922A (en) * | 2015-12-22 | 2016-04-20 | 虞涵晰 | Method for preparing selenium-enriched yeast autolysis powder through two-enzyme method |
| CN105581323A (en) * | 2015-12-22 | 2016-05-18 | 温州任和教育科技有限责任公司 | Preparation method of yeast powder high in nutritive value |
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