CN105925656A - Preparation method of rare-ginsenoside-Rg3-rich black ginseng - Google Patents
Preparation method of rare-ginsenoside-Rg3-rich black ginseng Download PDFInfo
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- 238000000034 method Methods 0.000 abstract description 9
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- 238000012545 processing Methods 0.000 abstract description 2
- XIRZPICFRDZXPF-UHFFFAOYSA-N Ginsenoside Rg3 Natural products CC(C)=CCCC(C)(O)C1CCC(C2(CC(O)C3C4(C)C)C)(C)C1C(O)CC2C3(C)CCC4OC1OC(CO)C(O)C(O)C1OC1OC(CO)C(O)C(O)C1O XIRZPICFRDZXPF-UHFFFAOYSA-N 0.000 abstract 2
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- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
- C12P33/20—Preparation of steroids containing heterocyclic rings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
- C12P33/005—Degradation of the lateral chains at position 17
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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Abstract
The invention relates to a preparation method of rare-ginsenoside-Rg3-rich black ginseng, belonging to the technical field of food processing. The method comprises the following steps: fermenting glutinous rice to prepare a fermentation liquid, carrying out ginseng fermentation, steaming and drying to obtain the rare-ginsenoside-Rg3-rich black ginseng product. The detection indicates that the ginsenoside Rg3 content in the black ginseng is 0.20%. The probiotics are utilized to ferment the target raw material so as to generate the specific invertase, and the enzymolysis catalytic action is utilized on the ginseng to obtain the rare-ginsenoside-Rg3-rich black ginseng product. The method is simple to operate, has the advantages of high efficiency and low cost, maintains the complete ginseng body, and obtains the ginsenoside-Rg3-rich black ginseng with low loss of other nutrients.
Description
Technical field:
The invention belongs to food-making technology field, relate to the preparation method of a kind of black ginseng.
Background technology:
In Radix Ginseng, principle active component is ginsenoside.Up to now, scientific research institution is from joining internal at least separation and Extraction to more than 40 kinds ginsenoside monomer.There are some rare saponin in ginsenoside monomer, its content in Radix Ginseng and Radix Ginseng is very little, it is necessary to its content just can be promoted to raise by ad hoc fashion Synthesis.
Ginsenoside Rg3It is exactly a kind of rare saponin, the ginsenoside Rg that existing scientific and technological level can be extracted from Radix Ginseng3Content is only 0.003%.Pharmacological research shows, Rg3The immunologic function of tumor-bearing mice can be significantly improved；Suppression intra-tumor new vessels is formed；Improve cardiovascular function, anti-platelet aggregation, protection cranial nerve cell, improve immunity of organisms, and there is the most anticancer, Anticancer metastasis action.
But it is existing to ginsenoside Rg3Extractive technique mainly by after Radix Ginseng is carried out pretreatment (such as broken, acid and alkali hydrolysis etc.), generating on the basis of panoxadiol organizes substrate, by the structural rearrangement to conventional saponin, and then extracting and prepare ginsenoside Rg3Monomer.If Cheng Leqin et al. (Chinese patent, application number 201310207242.3) is exactly preparation ginsenoside Rg on the basis of Radix Ginseng powder3.Yin Cheng et al. (Chinese patent, application number 200910217990.3) is then to utilize Alternaria bacterium that Radix Ginseng total saponins carries out fermentation preparation ginsenoside Rg3Monomer.Although said method can prepare ginsenoside Rg3Monomer, but all obtain on the basis of destroying Radix Ginseng configuration, other the precious compositions in Radix Ginseng are rejected, and cause great nutrition waste.Monomer saponin Rg after simultaneously preparing3Also being unfavorable for that consumer buys edible, actual development is worth and has a greatly reduced quality.
Black ginseng is the deep processed product of Radix Ginseng, gains the name because its ginseng body is brownish black.Black participation Radix Ginseng is compared, and has good raising immunity of organisms, anti-cancer, anticancer effect, simultaneously because its dry property significantly reduces, is more suitable for people of weak constitution group and eats.The black ginseng goods of tradition mainly shine technique by the steaming of nine iterative cycles and prepare, although also contain a certain amount of Rg3Composition, but it is complicated to there is process for integrally manufacturing, wastes time and energy, Rg3The drawback that content is unstable.
Summary of the invention:
The technical problem to be solved in the present invention is open a kind of rich in rare ginsenoside Rg3The preparation method of black ginseng.
It is to use following steps to complete that the present invention solves the scheme of technical problem:
1) preparation of fermentation liquid
Take processed after Oryza glutinosa soaks, add aspergillus oryzae by 0.2-0.8% weight ratio, then press 1-1.5% weight ratio access unartificial yeast, ferment 24-168 hour under the conditions of 35-45 DEG C, take its Oryza glutinosa fermented liquid standby；
Choose new fresh schisandra chinensis respectively and Fructus Crataegi is cleaned and smashs to pieces, mix according to the weight ratio of 5:1-2 and be placed in fermentation tank, add Oryza glutinosa fermented liquid, the weight of Oryza glutinosa fermented liquid is 5-10 times of weight after Fructus Schisandrae Chinensis and Fructus Crataegi mixing, access Lactobacillus plantarum, sealing and fermenting tank according to the ratio of feed liquid gross weight 0.2%-0.5%, temperature is set to 35-39 DEG C, ferment at constant temperature 72-120 hour, prepares fermented ginseng liquid；
2) preparation of fermented ginseng
The fresh ginseng cleaned up is placed in fermentation tank, adds fermented ginseng liquid according to the part by weight of 1:3-10, the temperature of fermentation tank is maintained at 35-39 DEG C, ferment at constant temperature 48-72 hour；
3) steam and be dried
After fermented ginseng completes, take out and be placed on High Temperature Rotating steaming ginseng case, under 80-100 DEG C of steam turbine cycles effect, steam ginseng body, steam in this way 4-6 hour, be then put in vacuum drier and be dried 2-4 hour；Temperature and time keep identical on the premise of, repetitive operation steams and drying steps twice, i.e. available rich in rare ginsenoside Rg3Black ginseng goods.
Water vapour is recycled by airtight reflux, and effective ingredient does not runs off.
After testing, ginsenoside Rg in the black ginseng prepared3Content is 0.20%.
The black ginseng goods making three batches according to such scheme compare Rg with Radix Ginseng3Content.By high efficiency liquid phase chromatographic analysis method, obtain following assay.And then proving that this technique content can be obviously improved and data stabilization, repeatability is high.
Assay
Wherein Oryza glutinosa is traditional staple food of the south China area common people, and it is rich in abundant amylopectin component, is suitable for making viscous foodstuff.Oryza glutinosa is nutritious, containing protein, fat, saccharide, calcium, phosphorus, ferrum, vitamin B1, vitamin B2, the composition such as nicotinic acid and starch.
Lactobacillus plantarum is the probiotic bacteria of a kind of anaerobic type, can produce specific lactobacillin and beta-glucosidase during it was bred, and existing science and technology proves that this enzyme has Radix Ginseng routine saponin Rb1Change into rare saponin Rg3Ability.This strain has been put into edible strain list, is a kind of safe and reliable lactic acid bacteria.
The present invention utilizes probiotic bacteria by target material fermentation is generated specific invertase, in Radix Ginseng and then obtains a kind of rich in rare ginsenoside Rg by enzymolysis catalytic action3Black ginseng goods and processing technology, the method is easy and simple to handle, efficient, low cost, both remained and completely join body, again rich in ginsenoside Rg3, other nutrient component damages are less simultaneously.
Detailed description of the invention:
Example 1, take processed after 100 kilograms of Oryza glutinosa soak, add 0.2 kilogram-metre aspergillosis, then access 1.2 kilograms of unartificial yeasts, ferment 48 hours under the conditions of 37 DEG C, take its Oryza glutinosa fermented liquid standby；
Take 5 kilograms of Fructus Schisandrae Chinensis and 1 kilogram of Fructus Crataegi, clean after blending, add 30 kilograms of Oryza glutinosa fermented liquids, access 0.072 kilogram of Lactobacillus plantarum, in fermentation tank under the conditions of 36 DEG C, ferment at constant temperature 80 hours, prepare fermentation liquid.
Take 2 kilograms of Radix Ginsengs cleanings to be placed in fermentation tank, add 6 kilograms of fermented ginseng liquid, under the conditions of 37 DEG C, ferment at constant temperature 96 hours.
Radix Ginseng after fermentation is placed in High Temperature Rotating steam in ginseng case, utilizes 80 DEG C of steam to steam 4 hours, be put in vacuum drier subsequently and be dried 2 hours.Repeat to steam and drying steps twice, i.e. can get 1.2kg rich in rare ginsenoside Rg3Black ginseng goods.
After testing, ginsenoside Rg in the black ginseng prepared3Content is 0.22%.
Example 2, taking processed after 100 kilograms of Oryza glutinosa soak, access 0.4 kilogram-metre aspergillosis, accesses 1.5 kilograms of unartificial yeasts, under the conditions of 39 DEG C, fermentation 36 hours, take its Oryza glutinosa fermented liquid standby；
Take 5 kilograms of Fructus Schisandrae Chinensis and 2 kilograms of Fructus Crataegis, clean after blending, add 49 kilograms of Oryza glutinosa fermented liquids, always re-access 56g Lactobacillus plantarum according to feed liquid, in fermentation tank under the conditions of 39 DEG C, ferment at constant temperature 96 hours, prepare fermentation liquid.
Take 3 kilograms of Radix Ginsengs cleanings to be placed in fermentation tank, add 6 kilograms of fermented ginseng liquid, under the conditions of 38 DEG C, ferment at constant temperature 48 hours.
Radix Ginseng after fermentation is placed in High Temperature Rotating steam in ginseng case, utilizes 100 DEG C of steam to steam 6 hours, be put in vacuum drier subsequently and be dried 4 hours.Repeat to steam and drying steps twice, i.e. can get 3.2kg rich in rare ginsenoside Rg3Black ginseng goods.
After testing, ginsenoside Rg in the black ginseng prepared3Content is 0.21%.
Example 3, taking processed after 100 kilograms of Oryza glutinosa soak, add 0.5 kilogram-metre aspergillosis, accesses 1.1% kilogram of unartificial yeast, under the conditions of 38 DEG C, fermentation 30 hours, take its Oryza glutinosa fermented liquid standby；
Take 5 kilograms of part Fructus Schisandrae Chinensis and 1.5 kilograms of Fructus Crataegis are cleaned and blend, add 52 kilograms of Oryza glutinosa fermented liquids, always re-access 40g Lactobacillus plantarum according to feed liquid, in fermentation tank under the conditions of 38 DEG C, ferment at constant temperature 90 hours, prepare fermented ginseng liquid.
Take 300 grams of Radix Ginsengs cleanings to be placed in fermentation tank, add 1.5 kilograms of fermentation liquids, under the conditions of 35 DEG C, ferment at constant temperature 45 hours.
Radix Ginseng after fermentation is placed in High Temperature Rotating steam in ginseng case, utilizes 95 DEG C of steam to steam 5 hours, be put in vacuum drier subsequently and be dried 3 hours.Repeat to steam and drying steps twice, i.e. can get 180g rich in rare ginsenoside Rg3Black ginseng goods.Black ginseng goods are pulverized, i.e. can get black ginseng powder.
After testing, ginsenoside Rg in the black ginseng powder prepared3Content is 0.25%.
Claims (2)
1. one kind rich in rare ginsenoside Rg3The preparation method of black ginseng, it is characterised in that by following steps
Complete:
1) preparation of fermentation liquid
Take processed after Oryza glutinosa soaks, add aspergillus oryzae by 0.2-0.8% weight ratio, then press 1-1.5% weight ratio
Access unartificial yeast, ferment 24-168 hour under the conditions of 35-45 DEG C, take its Oryza glutinosa fermented liquid standby；
Choose new fresh schisandra chinensis respectively and Fructus Crataegi is cleaned and smashs to pieces, mix to be placed according to the weight ratio of 5:1-2 and send out
In ferment tank, adding Oryza glutinosa fermented liquid, the weight of Oryza glutinosa fermented liquid is the 5-10 of weight after Fructus Schisandrae Chinensis and Fructus Crataegi mixing
Times, access Lactobacillus plantarum, sealing and fermenting tank according to the ratio of feed liquid gross weight 0.2%-0.5%, temperature is arranged
For 35-39 DEG C, ferment at constant temperature 72-120 hour, prepare fermented ginseng liquid；
2) preparation of fermented ginseng
The fresh ginseng cleaned up is placed in fermentation tank, adds Radix Ginseng according to the part by weight of 1:3-10 and send out
Ferment liquid, is maintained at 35-39 DEG C by the temperature of fermentation tank, ferment at constant temperature 48-72 hour；
3) steam and be dried
After fermented ginseng completes, take out and be placed on High Temperature Rotating steaming ginseng case, follow at 80-100 DEG C of steam turbine
Steam ginseng body under ring effect, steam in this way 4-6 hour, be then put in vacuum drier and be dried 2-4
Hour；Temperature and time keep identical on the premise of, repetitive operation steams and drying steps twice,
Obtain rich in rare ginsenoside Rg3Black ginseng goods.
Preparation method the most according to claim 1, it is characterised in that: water vapour is by airtight backflow dress
Put and recycle.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106361792A (en) * | 2016-11-02 | 2017-02-01 | 无锡西玛生物科技有限公司 | Improved production method of black ginseng |
| CN107259535A (en) * | 2017-07-12 | 2017-10-20 | 延边韩工坊健康制品有限公司 | A kind of high content Rg5 black ginseng product and preparation method |
| CN107929339A (en) * | 2017-12-28 | 2018-04-20 | 沈阳冠豪生物技术开发有限公司 | A kind of preparation method of black ginseng |
| CN108142924A (en) * | 2016-12-06 | 2018-06-12 | 张成中 | The preparation and its application of purplish red ginseng |
| CN114404465A (en) * | 2022-01-06 | 2022-04-29 | 解慧勇 | A black ginseng paste for treating tumor and its preparation method |
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| CN103536635A (en) * | 2012-07-16 | 2014-01-29 | 郑毅男 | Preparation method of holothuria nobilis and application thereof in treatment of diabetes mellitus |
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| CN108142924A (en) * | 2016-12-06 | 2018-06-12 | 张成中 | The preparation and its application of purplish red ginseng |
| CN107259535A (en) * | 2017-07-12 | 2017-10-20 | 延边韩工坊健康制品有限公司 | A kind of high content Rg5 black ginseng product and preparation method |
| CN107929339A (en) * | 2017-12-28 | 2018-04-20 | 沈阳冠豪生物技术开发有限公司 | A kind of preparation method of black ginseng |
| CN107929339B (en) * | 2017-12-28 | 2021-03-16 | 沈阳冠豪生物技术开发有限公司 | Preparation method of black ginseng |
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