CN103536635A - Preparation method of holothuria nobilis and application thereof in treatment of diabetes mellitus - Google Patents
Preparation method of holothuria nobilis and application thereof in treatment of diabetes mellitus Download PDFInfo
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Abstract
The invention provides a method for preparing holothuria nobilis from fresh ginseng, an effect of the holothuria nobilis on diabetes mellitus, and application of the holothuria nobilis in a drug for treating the diabetes mellitus. The holothuria nobilis is characterized by being prepared from the fresh ginseng by steps of repeatedly steaming by high-temperature steam for 2-9 times and drying. A diabetes mellitus animal model proves that the holothuria nobilis has the effects of reducing blood sugar level, improving glucose tolerance, regulating blood fat, protecting and repairing damaged islet after administration treatment of the holothuria nobilis. The substance can be applied to prevention and treatment of the diabetes mellitus.
Description
Technical field
The present invention relates to processing method and the application aspect treatment diabetes thereof of the black ginseng of processed goods of fresh ginseng.
Background technology
Diabetes and complication thereof are one of principal diseases threatening human health, and mortality rate is only second to tumor and cardiovascular disease, occupy at present the 3rd in various diseases.Diabetes are mainly divided into 1 type and type 2 diabetes mellitus, and type 2 diabetes mellitus accounts for 90%.In recent years, global diabetics number is increasing rapidly with surprising rapidity.Diabetes branch of Chinese Medical Association in 2010 shows about the result of China's diabetes prevalence investigation, in 20 years old above crowd of China, the prevalence of masculinity and femininity diabetes reaches respectively 10.6% and 8.8%, overall prevalence has reached 9.7%, according to the reckoning result of this survey result gained, shows that the ill total number of persons of China's diabetes reaches more than 9,200 ten thousand.It is predicted, global diabetics will increase to 3.8 hundred million examples of 2025 from 2.46 hundred million examples of 2006.At present, diabetes have become the main public health problem that has a strong impact on human lives and health.
At present, diabetes be take western medical treatment clinically as main, mainly contain the five large class medicines such as insulin, biguanides, Insulin secretagogues, euglycemic agent and alpha-glucosidase inhibitor.Though Western medicine can reduce blood sugar level, diabetes are had to certain therapeutical effect, long-term taking can produce serious side effects, especially gastrointestinal tract, kidney, liver, cardiovascular system etc. is all had to toxic and side effects in various degree.Chinese medicine is the treasure that the Chinese nation accumulates for thousands of years, and with respect to Western medicine, Chinese medicine is not only effective, and takes saferly, and toxic and side effects is little, and prolonged application treatment by Chinese herbs has obvious advantage aspect prevent diabetes complication.
Radix Ginseng is the traditional valuable ingredient of Chinese medicine of araliaceae ginseng plant ，Shi China.From ancient times as nourishing and fit keeping function, strengthening the body resistance, happy Fructus Alpiniae Oxyphyllae, make light of one's life by commiting suicide and prolong life top grade.Black ginseng is the deep processed product of fresh ginseng, through high-temperature steam repeatedly, is heated to black, therefore be called black ginseng, also has the report of manufacturing black ginseng with biological fermentation process abroad.After processing of Panax ginseng is black ginseng, after measured, its rare saponin content significantly raises, as ginsenoside Rg
3, Rg
5, Rk
1, CK etc., and there are some researches prove that this type of rare saponin has stronger antidiabetic effect at present.
Based on above research, this experiment is induced 1 type and type 2 diabetes mellitus animal model with streptozotocin (STZ), studies the impact of black ginseng on 1 type and type 2 diabetes mellitus mice, finds that black ginseng has good curative effect aspect the treatment of diabetes.Therefore, black ginseng is having considerable development and application to be worth aspect prevention and treatment diabetes medicament.
Through retrieval, have no black ginseng report aspect experiment in anti-1 type and type 2 diabetes mellitus animal body.
Summary of the invention
good effect of the present invention is:black ginseng has blood sugar lowering, improves glucose tolerance, regulates the effect of blood fat and protection and the impaired islets of langerhans of reparation.Black ginseng can be applicable to prevention and treatment diabetes.
Pharmaceutically acceptable carrier is conventional pharmaceutical carrier, comprises diluent, excipient, filler, adhesive, disintegrating agent, absorption enhancer, surfactant, absorption carrier, sweeting agent etc.
The present invention can be applied to the patient for the treatment of diabetes with the form of single or compositions by Oral administration.Also can be prepared into tablet, electuary, capsule.
Formulation rate of the present invention can be according to route of administration and combined situation, the variations such as patient age, body weight, disease and ill degree, and daily dose is 1-100mg/Kg.
Ginsenoside's containing quantity research in the black ginseng of experimental example 1
1 experiment material
Get Radix Ginseng Rubra, black ginseng sample (life in 6 years), self-control; Reference substance ginsenoside Rb
1, Rb
2, Rc, Rd, Re, Rf, Rg
1purchased from pharmaceutical college of Jilin University; Ginsenoside Rg
3, Rg
5, Compound K(CK) be the self-control of this laboratory, reference substance purity >98%.
2 instruments and reagent
| Shimadzu LC-20A high performance liquid chromatograph (SPD-20A UV-detector, LC-20AT pump, CTO-10AS VP column oven, LC-solution work station) | Japan Shimadzu company |
| The serial Rotary Evaporators of EYELA-DPE 2110 | Tokyo physics and chemistry apparatus Co., Ltd. |
| Sartorius BP211D type electronic analytical balance (100,000/) | Sartorius AG |
| KQ-250DB type numerical control ultrasonic cleaner | Kunshan ultrasonic instrument company limited |
| FW135 crusher for Chinese herbal medicine | Tianjin Stettlen Instrument Ltd. |
| The automatic dual pure water distillator of SZ-93 | Shanghai Yarong Biochemical Instrument Plant |
| Acetonitrile (chromatographically pure) | U.S. Tedia company |
| Methanol (analytical pure) | Beijing Chemical Plant |
| Water is the dual distilled water of self-control | ? |
3 methods and result
The preparation of 3.1 need testing solutions
Get respectively the Radix Ginseng Rubra after pulverizing, black ginseng sample powder (crossing 80 mesh sieves) approximately 1 g, accurately weighed.Above sample is put in tool plug conical flask, added 50 mL methanol, ultrasonic (250 W, 40 kHz) extract 2 times, and each 30 min filter, and merge filtrate 2 times, concentrating under reduced pressure, and with in methanol constant volume to 25 mL measuring bottle, shake up, cross 0.45 μ m filter membrane, obtain.
3.2 chromatographic condition
Yi Lite Hypersil ODS2(4.6 mm * 250 mm, 5 μ m) chromatographic column; Mobile phase: acetonitrile-water binary gradient elution; Flow velocity: 1 mL/min; Detect wavelength: 203 nm; Column temperature: 30 ℃; Sample size 20 μ L.Gradient elution program is in Table 2-1.
Table 1 Radix Ginseng Rubra and black ginseng eluent gradient eluting table
Tab.2-2?Gradient?elution?of?mobile?phase?of?red?and?black?ginseng
| Time (min) | Acetonitrile (%) | Water (%) |
| 0 | 19 | 81 |
| 25 | 20 | 80 |
| 36 | 31 | 69 |
| 60 | 40 | 60 |
| 90 | 80 | 20 |
| 95 | 100 | 0 |
3.3 ginsenoside's measurement results
Fresh ginseng and processed goods saponin content thereof the results are shown in Table 2.Radix Ginseng Rubra and black ginseng are the hot-working product of Radix Ginseng, and total saponins is respectively 2.962%, 5.534%, and the more undressed Radix Ginseng of its contained saponin kind increases, ginsenoside Rg
3, Rg
5, the rare saponin content of CK increases gradually, in black ginseng, conventional saponin content is extremely low, ginsenoside Rg
3, Rg
5, CK content significantly increases.
the black ginseng manufacture method of experimental example 2
Get bright ginseng and clean, remove its foreign material, put into drying baker 60-70 ℃ of dry 8-10h, after be dried in high-temperature steam 95-115 ℃ steam 2h, after having steamed, in 60-70 ℃ of dry 6-10h, 9 times so repeatedly, it is below 15% that taking-up is dried to water content, obtains black ginseng.Analyze after measured ginsenoside Rg in black ginseng
3, Rg
5, Rk
1, the rare saponin content such as CK significantly increases, and in fresh ginseng, this type of saponin content is few.The content of finding ginsenoside in the black ginseng of different processing number of times in ginsenoside's detection all changes, and comprises that the total Ginsenosides Content of rare saponin is between 1%-7%.
the anti-type 1 diabetes of the black ginseng of experimental example 3
1 experimental technique
Get healthy male mouse of kunming some, adaptability is raised one week, get at random 10 as blank group, all the other mice fasting be can't help after water 12 h, lumbar injection STZ 150 mg/kg(are dissolved in the citric acid buffer salt solution of the new preparation of 0.1 M, and pH 4.5), after 6 days, measure fasting glucose, the mice of blood glucose value >=8.00 mmol/L is as modeling success mice.The successful mice of modeling is divided into 3 groups at random, 10 every group.Dosage is insulin 10U/Kg, black ginseng 900mg/Kg, and normal group and model group are given isopyknic placebo.Be unified in every morning 8:00-9:00 and be administered once, successive administration 4 weeks, records Mouse Weight, food ration, amount of drinking water before administration every day.
After mice administration 4 weeks, each organizes mouse assay fasting glucose.After mice fasting 12 h (can't help water), eye socket rear vein beard is got blood, centrifugal 10 min of 3500 r/min, and separation of serum, by glucose kit method, in 96 orifice plate application of samples, microplate reader is measured, and calculates mice 12h blood glucose on an empty stomach.
Mice administration 4 weeks, after administration 2 h, each is organized mice empty stomach gavage and gives glucose 2 g/kg the last time, and mouse orbit rear vein beard is got blood, measures respectively 0 min, 30 min, 60 min, 120 min blood glucose values, and area (AUC) under calculated curve.Evaluate and respectively organize the impact of medicine on type 1 diabetes mice oral glucose tolerance.
2 data statisticss
Data result represents with mean+SD.With SPSS17.0 software, carry out statistical analysis, with ANOVA variance analysis, carry out deal with data, * * represents and the poor heteropole of model group remarkable (P < 0.01);
##represent and the poor heteropole of normal group remarkable (P < 0.01).
3 experimental results
3.1 impacts of black ginseng on type 1 diabetes mice fasting glucose
After administration 4 weeks, mice fasting blood glucose level obviously improves, as shown in Figure 1.After injection STZ, diabetic mice compared with normal group mouse blood sugar level obviously raises.Model group is with placebo administration after 4 weeks, and blood glucose value raises to some extent; Insulinize group mice blood glucose after the treatment of 4 weeks is tending towards normally, and black ginseng group mice fasting glucose obviously declines, and blood glucose value is down to 7.11 ± 1.28 mmol/L, approaches normal level, compared with model group difference extremely significantly (p<0.01).Therefore, black ginseng hypoglycemic effect is good.
The impact of 3.2 black ginsengs on type 1 diabetes mice oral glucose tolerance
After mice administration 4 weeks, post-prandial glycemia curve as shown in Figure 2, is compared with model group mice, and each treatment group mice oral glucose tolerance all has clear improvement.As shown in Figure 3, model group area under curve (AUC) is apparently higher than blank group, and difference is (p<0.01) extremely significantly; With respect to model group, the AUC range of decrease of black ginseng group is 36.65%, and difference is (p<0.01) extremely significantly.Therefore, black ginseng can effectively be improved the oral glucose tolerance of type 1 diabetes mice, and effect is remarkable.
the anti-type 2 diabetes mellitus of the black ginseng of experimental example 4
1 experimental technique
Get healthy male ICR mouse some, normal feedstuff adaptability is raised one week, get at random 10 as blank group, all the other mices are given high lipid food (fat 22%, protein 20%, carbohydrate 48%, other compositions that comprise cellulose and moisture account for 10%, total amount of heat is 44.3 kJ/kg) raising surrounding, mice fasting be can't help after water 12 h, lumbar injection streptozotocin 100 mg/kg(are dissolved in the citric acid buffer salt solution of the new preparation of 0.1 M, pH 4.5), continuation with fed with high surrounding after, measure fasting glucose, blood glucose value higher than the mice of 8.00 mmol/L as type 2 diabetes mellitus modeling success mice.The successful mice of modeling is divided into 3 groups at random, 10 every group.Dosage is respectively metformin 250 mg/kg, black ginseng 900 mg/kg, and normal group and model group are given isopyknic placebo.Be unified in every morning 8:00-9:00 and be administered once, successive administration 6 weeks, records Mouse Weight, food ration, amount of drinking water before administration every day.
After administration 6 weeks, each organizes mouse assay fasting glucose.After mice fasting 12 h (can't help water), eye socket rear vein beard is got blood, centrifugal 10 min of 3500 r/min, and separation of serum, by glucose kit method, in 96 orifice plate application of samples, microplate reader is measured, and calculates mice 12 h blood glucose on an empty stomach.
After administration 6 weeks, each is organized mice empty stomach gavage and gives glucose 2 g/kg, measures respectively 0 min, 30 min, 60 min, 120 min blood glucose values, and area under calculated curve.Evaluate and respectively organize the impact of medicine on type 2 diabetes mellitus mice oral glucose tolerance.
After administration 6 weeks, each is organized eyeball of mouse and gets blood, centrifugal 10 min of 3500 r/min, and separation of serum, according to TC, TG, HDL-C, LDL-C test kit method, respectively at 96 orifice plate application of samples, microplate reader is measured, and calculates mice serum TC, TG, HDL-C, LDL-C level.
After administration 6 weeks, each is organized the dislocation of mice cervical vertebra and puts to death, and takes out rapidly pancreas, is fixed in 4% formaldehyde, soaks 24-48h.By internal organs, through gradient ethanol dehydration, the ethanol with in dimethylbenzene replacement tissue, makes it transparent, puts into the paraffin waxdip of melting and removes bubble, then immersing cooled and solidified in new paraffin.By the wax stone section of fixing, carry out hematoxylin-eosin (HE) dyeing, after dyeing, then carry out gradient ethanol dehydration, the ethanol in dimethylbenzene replacement tissue sheet, neutral gum mounting.Micro-Microscopic observation pancreas form.
2 data statisticss
Data result represents with mean+SD.With SPSS17.0 software, carry out statistical analysis, with ANOVA variance analysis, carry out deal with data, * represent and model group comparing difference remarkable (P < 0.05), * * represents and the poor heteropole of model group remarkable (P < 0.01); # represents and normal group comparing difference significantly (P < 0.05), and ## represents and the poor heteropole of normal group significantly (P < 0.01).
3 experimental results
3.1 impacts of black ginseng on type 2 diabetes mellitus mice fasting glucose
After administration 6 weeks, the result of mice fasting blood glucose level as shown in Figure 4.Compare with model group, black ginseng group and positive drug metformin group blood glucose all have obvious decline, wherein, black ginseng group blood glucose significantly declines, model group mouse blood sugar reaches 18.19 mmol/L, black ginseng group blood glucose has reduced by 20.18%, and black ginseng group is compared with model group, has significant difference (p<0.05).Therefore, black ginseng all has certain hypoglycemic activity to type 2 diabetes mellitus mice, and effect is better than metformin.
The impact of 3.2 black ginsengs on type 2 diabetes mellitus mice oral glucose tolerance
Through the treatment of 6 weeks, post-prandial glycemia curve as shown in Figure 5, was compared with model group mice, and each treatment group mice oral glucose tolerance all has clear improvement.As shown in Figure 6, model group area under curve (AUC) is apparently higher than matched group (p < 0.01), with respect to model group (AUC is 2796.45 ± 287.53 mmolmin/L), metformin group AUC has declined 25.07%, and black ginseng group AUC has declined 20.03%, and with respect to model group significant difference (p < 0.05).Therefore, black ginseng can significantly be improved type 2 diabetes mellitus mice oral glucose tolerance.
3.3 impacts of black ginseng on type 2 diabetes mellitus lipid of mice index
After administration 6 weeks, lipid of mice index is as shown in table 1.Model group mice serum cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) level are all significantly higher than matched group, and HDL-C (HDL-C) level is significantly lower than matched group.Black ginseng can reduce type 2 diabetes mellitus mice serum TC and TG level, and serum TC level has trend and the effect of reduction generally, but not statistically signigicant; And relative model group under black ginseng group TG level 26.40%, has significant difference (p < 0.05); In addition, can raise type 2 diabetes mellitus mice serum HDL-C level reduce LDL-C level of black ginseng, with respect to model group, black ginseng group HDL-C level raises 26.02%, has significant difference (p < 0.05).Therefore, black ginseng has the effect that regulates type 2 diabetes mellitus lipid of mice, can reduce mice serum TC, TG, LDL-C level, elevating HDL-C level, and action effect is better than positive drug metformin.
The impact (Mean ± SD, mmol/L) of the black ginseng type 2 diabetes mellitus of table 1 lipid of mice index
| Group | TC | TG | HDL-C | LDL-C |
| Matched group | 2.46±0.45 | 0.72±0.11 | 2.73±0.33 | 2.07±0.37 |
| Model group | 3.72±0.81 | 1.32±0.28 # | 1.96±0.31 | 2.87±0.52 |
| Metformin | 3.18±0.68 | ?1.15±0.25 * | 2.25±0.28 | 2.27±0.41 |
| Black ginseng | 3.11±0.47 | ?0.97±0.21 * | ?2.47±0.27 * | 2.55±0.46 |
#p < 0.05 vs matched group,
*p < 0.05 vs model group
3.4 impacts of black ginseng on type 2 diabetes mellitus mice pancreatic tissue
Accompanying drawing 7 is shown in pancreatic tissue HE dyeing.Compare with Normal group, the islets of langerhans atrophy in type 2 diabetes mellitus mice pancreatic, islet area reduces, and islets of langerhans b cell quantity reduces, and in the middle of islets of langerhans, has cavity, and edge is imperfect, and islet cells is arranged mixed and disorderly, and inflammatory cell infiltration is obvious.After Or Metformin In Treating, compare with model group, islets of langerhans has inflammatory cell infiltration, and islets of langerhans b cell number increases, and edge is comparatively complete; After black ginseng treatment, compare and make moderate progress with model group, islets of langerhans has inflammatory infiltration, and islets of langerhans b cell number increases, and edge is more complete, and islet area obviously increases compared with model group.
accompanying drawing explanation
The impact of the black ginseng of accompanying drawing 1 on type 1 diabetes mice fasting glucose
## p < 0.01 vs matched group, * * p < 0.01 vs model group
Accompanying drawing 2 type 1 diabetes mice oral glucose tolerance figure
The impact of the black ginseng of accompanying drawing 3 on type 1 diabetes mice oral glucose tolerance area under curve (AUC)
## p < 0.01 vs matched group, * * p < 0.01 vs model group
The impact of the black ginseng of accompanying drawing 4 on type 2 diabetes mellitus mice fasting glucose
## p < 0.01 vs matched group, * * p < 0.01 vs model group
Accompanying drawing 5 type 2 diabetes mellitus mice oral glucose tolerance figure
The impact of the black ginseng of accompanying drawing 6 on type 2 diabetes mellitus mice oral glucose tolerance area under curve (AUC)
## p < 0.01 vs matched group, * * p < 0.01 vs model group
Accompanying drawing 7 type 2 diabetes mellitus mice pancreatics are organized HE dyeing (* 200)
A. matched group; B. model group; C. metformin; D. black ginseng
Saponin reference substance HPLC figure in the black ginseng of accompanying drawing 8
1,Rg
1;?2,Re;?3,Rf;?4,Rg
2;?5,Rb
1;?6,Rc;?7,Rb
2;?8,Rb
3;?9,Rd;?10,Rg
3;?11,Compound?K;?12,Rg
5
5 specific embodiments
the manufacture method of embodiment 1 black ginseng:get bright ginseng and clean, remove foreign material, put into 60 ℃ of dry 8h of drying baker, after be dried in high-temperature steam 115 ℃ steam 2h, after having steamed, in 60-70 ℃ of dry 8h, it is below 15% that taking-up is dried to water content, obtains black ginseng 1.Through HPLC determination and analysis total saponins 21.06 ± 0.98 mg/g, wherein rare saponin Rg
3, Compound K and Rg
5be respectively 0.52 ± 0.01,0,35 ± 0.01 and 2.72 ± 0.02 (mg/g).
the manufacture method of embodiment 2 black ginsengs:get bright ginseng and clean, remove foreign material, put into 60 ℃ of dry 8h of drying baker, after be dried in high-temperature steam 115 ℃ of steaming 2h, after having steamed, in 60-70 ℃ of dry 8h, so repeat 2 times, obtain black ginseng 2.Through HPLC determination and analysis total saponins 50.28 ± 1.29 mg/g, wherein rare saponin Rg
3, Compound K and Rg
5be respectively 3.52 ± 0.03,3.61 ± 0.03 and 27.51 ± 0.12 (mg/g).
the manufacture method of embodiment 3 black ginsengs:get bright ginseng and clean, remove foreign material, put into 60 ℃ of dry 8h of drying baker, after be dried in high-temperature steam 115 ℃ of steaming 2h, after having steamed, in 60-70 ℃ of dry 8h, so repeat 3 times, obtain black ginseng 3.Through HPLC determination and analysis total saponins 65.50 ± 1.69 mg/g, wherein rare saponin Rg
3, Compound K and Rg
5be respectively 5.42 ± 0.04,5.61 ± 0.04 and 65.51 ± 1.65 (mg/g).
the manufacture method of embodiment 4 black ginsengs:get bright ginseng and clean, remove foreign material, put into 60 ℃ of dry 8h of drying baker, after be dried in high-temperature steam 115 ℃ of steaming 2h, after having steamed, in 60-70 ℃ of dry 8h, so repeat 4 times, obtain black ginseng 4.Through HPLC determination and analysis total saponins 79.45 ± 1.11 mg/g, wherein rare saponin Rg
3, Compound K and Rg
5be respectively 7.52 ± 0.04,7.16 ± 0.06 and 79.51 ± 1.12 (mg/g).
the manufacture method of embodiment 5 black ginsengs:get bright ginseng and clean, remove foreign material, put into 60 ℃ of dry 8h of drying baker, after be dried in high-temperature steam 115 ℃ of steaming 2h, after having steamed, in 60-70 ℃ of dry 8h, so repeat 5 times, obtain black ginseng 5.Through HPLC determination and analysis total saponins 67.98 ± 1.29 mg/g, wherein rare saponin Rg
3, Compound K and Rg
5be respectively 7.08 ± 0.04,5.88 ± 0.04 and 50.78 ± 0.29 (mg/g).
the manufacture method of embodiment 6 black ginsengs:get bright ginseng and clean, remove foreign material, put into 60 ℃ of dry 8h of drying baker, after be dried in high-temperature steam 115 ℃ of steaming 2h, after having steamed, in 60-70 ℃ of dry 8h, so repeat 6 times, obtain black ginseng 6.Through HPLC determination and analysis total saponins, 80.93 ± 0.45 mg/g, wherein rare saponin Rg
3, Compound K and Rg
5be respectively 6.78 ± 0.05,6.75 ± 0.04 and 50.78 ± 0.29 (mg/g).
the manufacture method of embodiment 7 black ginsengs:get bright ginseng and clean, remove foreign material, put into 60 ℃ of dry 8h of drying baker, after be dried in high-temperature steam 115 ℃ of steaming 2h, after having steamed, in 60-70 ℃ of dry 8h, so repeat 5 times, obtain black ginseng 7.Through HPLC determination and analysis, total saponins 75.63 ± 2.00 mg/g, wherein rare saponin Rg
3, Compound K and Rg
5be respectively 5.96 ± 0.05,6.09 ± 0.04 and 59.78 ± 0.49 (mg/g).
the black ginseng blood sugar lowering of embodiment 8 capsule
Get the black ginseng powder of embodiment 4 (crossing 80-200 mesh sieve), incapsulate, every containing black ginseng 0.1 ~ 0.5g.Other requirements meet the relevant regulations of 2010 editions relevant capsules of < < Pharmacopoeia of People's Republic of China > >.
the black ginseng blood sugar lowering of embodiment 9 tablet
Get the black ginseng powder of embodiment 4 (crossing 80-200 mesh sieve) 5g, cyclodextrin 20g, dry starch is appropriate, and carboxymethyl cellulose is appropriate, mixes, and pelletizing press sheet, makes the heavy 0.50g of tablet, containing black ginseng 5%-20% (g/g).Other requirements meet the relevant regulations of 2010 editions relevant tablets of < < Pharmacopoeia of People's Republic of China > >.
the black ginseng blood sugar lowering of embodiment 10 effervescent tablet
Get the black ginseng powder of embodiment 4 (crossing 80-200 mesh sieve) 10g, add excipient appropriate, mix homogeneously, pelletize tabletting, makes effervescent tablet, and every containing black ginseng 5-20% (g/g).Sundry item meets the related application of 2010 editions relevant effervescent tablets of < < Pharmacopoeia of People's Republic of China > >.
the black ginseng blood sugar lowering of embodiment 11 concentrated pill
Get the black ginseng powder of embodiment 4 (crossing 80-200 mesh sieve) 5g, cyclodextrin 20g, carboxymethyl cellulose is appropriate, mixes, pulverize, pure water, the general ball of making, every containing ginsenoside M1 fatty-acid monoester 5-20% (g/g).Other requirements meet the relevant regulations of 2010 editions relevant pills of < < Pharmacopoeia of People's Republic of China > >.
the black ginseng blood sugar lowering of embodiment 12 granule
Get the black ginseng powder of embodiment 4 (crossing 80-200 mesh sieve) 10g, soluble starch 200g, Icing Sugar is appropriate, and magnesium stearate 3g mixes, granule processed, the packing of composite aluminium plastic bag, loading amount 5.0g/ bag.Other requirements meet the relevant regulations of 2010 editions relevant granules of < < Pharmacopoeia of People's Republic of China > >.
the black ginseng Hypoylycemic health tea of embodiment 13
Get the black ginseng powder of embodiment 4 (crossing 80-200 mesh sieve) 10g, dextrin 50g, soluble starch 50g, Icing Sugar is appropriate, mixes tea making piece, minute packing, loading amount 5.0g/ bag.Other requirements meet the relevant regulations of 2010 editions relevant medicinal tea of < < Pharmacopoeia of People's Republic of China > >.
the black ginseng auxiliary hyperglycemic function of embodiment 14 cookies
Get the black ginseng powder of embodiment 4 (crossing 100 mesh sieves) 10g, edible crude fibre 100g, starch 100g, edible essence is appropriate, and food coloring is appropriate, and drinking pure is appropriate, mixes, and molding, cures, packing.Other requirements meet the related request of functions of the state food.
Claims (4)
1. a black ginseng preparation method with antidiabetic effect, a kind of method of preparing black ginseng from fresh ginseng
,concrete grammar: bright ginseng is cleaned, and removes its foreign material, puts into drying baker 60-70 ℃ of dry 8-10h, after being dried in high-temperature steam 95-115 ℃ steam 2h, after having steamed in 60-70 ℃ of dry 6-10h, 2-9 time so repeatedly, it is below 15% that taking-up is dried to water content, obtains black ginseng.
2. according to the black ginseng of claim 1 gained, there is blood sugar lowering, improve glucose tolerance, regulate the effect of blood fat and protection and the impaired islets of langerhans of reparation.
3. according to the black ginseng of claim 1 gained, have antidiabetic effect, black ginseng can be used for making the medicine of prevention or treatment diabetes.
4. pharmaceutical composition, wherein contains material and pharmaceutically acceptable carrier and dosage form described in claim 1.
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| CN107296279A (en) * | 2017-07-12 | 2017-10-27 | 延边韩工坊健康制品有限公司 | Black ginseng oral liquid and preparation method rich in the fatigue-relieving of ginsenoside Rg 5 |
| CN113797237A (en) * | 2021-10-14 | 2021-12-17 | 天津科技大学 | Method for extracting ginsenoside |
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2012
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| CN103800413A (en) * | 2014-03-13 | 2014-05-21 | 吉林农业大学 | Compound scrophulariahenryi hemsl powder and preparation process thereof |
| WO2016110168A1 (en) * | 2015-01-09 | 2016-07-14 | 富力 | Applications of 20(r)-ginsenosides rg3 in preparing medicament or healthcare product for relief and/or treatment of diabetes and medicament |
| JP2016150910A (en) * | 2015-02-17 | 2016-08-22 | 日本メナード化粧品株式会社 | Endurance-improving agent |
| CN105925656A (en) * | 2016-06-30 | 2016-09-07 | 吉林厚存生物科技股份有限公司 | Preparation method of rare-ginsenoside-Rg3-rich black ginseng |
| CN106333365A (en) * | 2016-09-08 | 2017-01-18 | 吉林医药学院 | Hovenia acerba and ginseng effervescent tablet and preparation method |
| CN107259535A (en) * | 2017-07-12 | 2017-10-20 | 延边韩工坊健康制品有限公司 | A kind of high content Rg5 black ginseng product and preparation method |
| CN107296279A (en) * | 2017-07-12 | 2017-10-27 | 延边韩工坊健康制品有限公司 | Black ginseng oral liquid and preparation method rich in the fatigue-relieving of ginsenoside Rg 5 |
| CN113797237A (en) * | 2021-10-14 | 2021-12-17 | 天津科技大学 | Method for extracting ginsenoside |
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