CN114680085A - Method for cultivating colored silkworms in greenhouse - Google Patents
Method for cultivating colored silkworms in greenhouse Download PDFInfo
- Publication number
- CN114680085A CN114680085A CN202011584935.0A CN202011584935A CN114680085A CN 114680085 A CN114680085 A CN 114680085A CN 202011584935 A CN202011584935 A CN 202011584935A CN 114680085 A CN114680085 A CN 114680085A
- Authority
- CN
- China
- Prior art keywords
- silkworms
- colored
- greenhouse
- stirring
- cultivating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title claims abstract description 21
- 240000000249 Morus alba Species 0.000 claims abstract description 22
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 22
- 238000005286 illumination Methods 0.000 claims abstract description 14
- 229930003231 vitamin Natural products 0.000 claims abstract description 12
- 235000013343 vitamin Nutrition 0.000 claims abstract description 12
- 239000011782 vitamin Substances 0.000 claims abstract description 12
- 229940088594 vitamin Drugs 0.000 claims abstract description 12
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 8
- 230000000384 rearing effect Effects 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims description 44
- 244000157072 Hylocereus undatus Species 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 238000002156 mixing Methods 0.000 claims description 18
- 235000010837 Echinocereus enneacanthus subsp brevispinus Nutrition 0.000 claims description 17
- 235000006850 Echinocereus enneacanthus var dubius Nutrition 0.000 claims description 17
- 244000005700 microbiome Species 0.000 claims description 17
- 239000008367 deionised water Substances 0.000 claims description 15
- 229910021641 deionized water Inorganic materials 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 15
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- 235000013379 molasses Nutrition 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 235000018481 Hylocereus undatus Nutrition 0.000 claims description 8
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 238000009210 therapy by ultrasound Methods 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 5
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 5
- 239000004571 lime Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000004537 pulping Methods 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 229930003756 Vitamin B7 Natural products 0.000 claims description 4
- 229930003761 Vitamin B9 Natural products 0.000 claims description 4
- 235000011912 vitamin B7 Nutrition 0.000 claims description 4
- 239000011735 vitamin B7 Substances 0.000 claims description 4
- 235000019159 vitamin B9 Nutrition 0.000 claims description 4
- 239000011727 vitamin B9 Substances 0.000 claims description 4
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 3
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 3
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 3
- 229930003451 Vitamin B1 Natural products 0.000 claims description 3
- 229930003268 Vitamin C Natural products 0.000 claims description 3
- 241000235015 Yarrowia lipolytica Species 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 3
- 229960003495 thiamine Drugs 0.000 claims description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 3
- 235000010374 vitamin B1 Nutrition 0.000 claims description 3
- 239000011691 vitamin B1 Substances 0.000 claims description 3
- 235000019154 vitamin C Nutrition 0.000 claims description 3
- 239000011718 vitamin C Substances 0.000 claims description 3
- 241000186840 Lactobacillus fermentum Species 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 2
- 229940012969 lactobacillus fermentum Drugs 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 10
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 4
- 238000004043 dyeing Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000007639 printing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003157 biological pigment Substances 0.000 description 1
- 125000004057 biotinyl group Chemical group [H]N1C(=O)N([H])[C@]2([H])[C@@]([H])(SC([H])([H])[C@]12[H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C(*)=O 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 125000003929 folic acid group Chemical group 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/143—Fermentum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for cultivating colored silkworms in a greenhouse, which comprises the following steps: firstly, primary rearing of color young silkworms: soaking folium Mori in treating solution, performing illumination treatment with purple light with illumination intensity of 260-270Lx for 10-20min, and feeding color young silkworm with folium Mori until the young silkworm reaches 1-2 years old. According to the colored silkworm breeding method, colored young silkworms are firstly bred, mulberry leaves are sent into a treatment solution for soaking after the primary breeding, and then are irradiated by purple light with the illumination intensity of 260Lx, so that the obtained mulberry leaves have high vitamin content and antibacterial capacity, and the survival capacity of the newly bred silkworms can be improved.
Description
Technical Field
The invention relates to the technical field of greenhouse cultivation, in particular to a cultivation method for cultivating colored silkworms in a greenhouse.
Background
At present, silk products with various bright colors on the market are mostly obtained by carrying out post-treatment processes such as refining, dyeing, printing and the like on white silkworm cocoons after boiling, reeling and weaving silk. Although the silk is rich in color and stable in color, the used chemical dyes pollute the environment, influence the wearing comfort and are not in accordance with the concept of green environmental protection. The pigment of the natural colored silk comes from biological pigment synthesized in the body of the silkworm, is nontoxic and harmless, has soft and bright color, does not need dyeing on fabrics, and avoids pollution caused by printing and dyeing.
The prior color silkworms have low breeding efficiency and low silk yield, which limits breeding technology, thereby requiring further improved treatment.
Disclosure of Invention
The invention aims to provide a method for cultivating colored silkworms in a greenhouse, which solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention provides a method for cultivating colored silkworms in a greenhouse, which comprises the following steps:
firstly, primary rearing of color young silkworms: soaking mulberry leaves in a treatment solution, then carrying out illumination treatment for 10-20min by adopting purple light with illumination intensity of 260-270Lx, and feeding color young silkworms with the mulberry leaves until the color young silkworms reach 1-2 instars;
step two, building a greenhouse: building a greenhouse, scattering a layer of lime powder on the ground of the greenhouse, transferring the colored young silkworms into the greenhouse, and feeding the mulberry leaves for 2-3 times every day until the 3 rd instar;
step three, preparing a nutritional agent: mixing molasses, K2HPO4 inorganic salt and microorganism according to the weight ratio of 3:2:1, stirring, adding deionized water 2 times of the total amount of molasses, and stirring for 10-20 min;
step four, mulberry leaf treatment and feeding: spraying nutrient on folium Mori, and feeding colored silkworm until the age of colored silkworm is 4-5.
Preferably, the preparation method of the treatment fluid comprises the following steps: 5-10 parts of vitamin is placed in 20-30 parts of 10-20% sodium chloride solution by mass for stirring for 10-20min, then 1-5 parts of pitaya peel extracting agent is added, and then stirring is carried out for 10-20min at the rotating speed of 100-200r/min, and the treatment fluid is obtained after the stirring is finished.
Preferably, the vitamin is one or more of vitamin B11, vitamin B7, vitamin C and vitamin B1.
Preferably, the preparation method of the pitaya peel extracting agent comprises the following steps:
s1: cleaning pitaya peel with clear water, adding deionized water which is 1-3 times of the weight of the pitaya peel, pulping, adding cellulase, stirring and performing enzymolysis for 3-4 hours to obtain an enzymolysis material;
s2: filtering and centrifuging the enzymolysis material, adding deionized water, boiling, filtering to obtain filtrate, and mixing the filtrates;
s3: adding the filter residue into 1-2 times of 75-80% alcohol solution, ultrasonic treating, centrifuging, mixing all centrifugates, and mixing with the filtrate in S2 to obtain the dragon fruit peel extract.
Preferably, the temperature of stirring enzymolysis is 35-40 ℃, and the stirring speed is 50-100 r/min.
Preferably, the temperature of stirring enzymolysis is 37.5 ℃, and the stirring speed is 75 r/min.
Preferably, the ultrasonic conditions in S3 are: ultrasonic treatment at 20-30kHz for 55-65 min.
Preferably, the microorganism is one of lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus fermentum, candida lipolytica and wine yeast.
Preferably, the inoculation amount of the microorganism is 2-4%.
Preferably, the inoculation amount of the microorganism is 3%.
Compared with the prior art, the invention has the following beneficial effects:
according to the color silkworm breeding method, color young silkworms are bred firstly, mulberry leaves are fed into a treatment solution for soaking after the initial breeding, then the mulberry leaves are irradiated by purple light with the illumination intensity of 260Lx, the obtained mulberry leaves have high vitamin content and antibacterial capacity, so that the survival capacity of the newly bred silkworms can be improved, a foundation is laid for subsequent greenhouse breeding, the growth requirements of the color silkworms are met by spraying the mulberry leaves with a nutritional agent in different breeding methods of 3-year and 4-year stages in the greenhouse breeding, and the bred silkworms can produce high-quality silks and have high growth activity, so that the production yield of the silkworms is improved.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1:
the method for cultivating the colored silkworms in the greenhouse comprises the following steps:
firstly, primary rearing of color young silkworms: soaking folium Mori in the treating solution, performing illumination treatment with purple light with illumination intensity of 260Lx for 10min, and feeding color young silkworm with folium Mori until the young silkworm reaches 1 year of age;
step two, building a greenhouse: building a greenhouse, scattering a layer of lime powder on the ground of the greenhouse, transferring the colored young silkworms into the greenhouse, and feeding the mulberry leaves for 2 times every day till the 3 rd instar;
step three, preparing a nutrient: mixing molasses, K2HPO4 inorganic salt and microorganism according to the weight ratio of 3:2:1, stirring, adding deionized water 2 times of the total amount of molasses, and stirring for 10 min;
step four, mulberry leaf treatment and feeding: spraying nutrient on folium Mori, and feeding colored silkworm until the age of colored silkworm is 4.
The preparation method of the treatment solution of this example was: and (2) placing 5 parts of vitamin into 20 parts of 10% sodium chloride solution by mass fraction, stirring for 10min, then adding 1 part of pitaya peel extracting agent, stirring for 10min at the rotating speed of 100r/min, and obtaining the treatment solution after stirring.
The vitamin in this example is vitamin B11.
The preparation method of the pitaya peel extracting agent comprises the following steps:
s1: cleaning dragon fruit peel with clear water, adding deionized water 1-3 times of the weight of the dragon fruit peel, pulping, adding cellulase, stirring and performing enzymolysis for 3h to obtain an enzymolysis material;
s2: filtering and centrifuging the enzymolysis material, adding deionized water, boiling, filtering to obtain filtrate, and mixing the filtrates;
s3: adding the filter residue into 1 time of 75% alcohol solution by volume fraction, performing ultrasonic treatment, centrifuging, combining all centrifugates, and then combining with the filtrate in S2 to obtain the pitaya peel extractant.
The temperature of stirring enzymolysis in this embodiment is 35 ℃, and the stirring rotation speed is 50 r/min.
The ultrasonic conditions in S3 of the present example were: 20kHz ultrasonic wave is carried out for 55 min.
The microorganism of this example is lactobacillus plantarum.
The inoculum size of the microorganism of this example was 2%.
Example 2:
the method for cultivating the colored silkworms in the greenhouse comprises the following steps:
firstly, primary rearing of color young silkworms: soaking folium Mori in the treating solution, performing illumination treatment with purple light with illumination intensity of 270Lx for 20min, and feeding color young silkworm with folium Mori until the young silkworm reaches 2 years old;
step two, building a greenhouse: building a greenhouse, scattering a layer of lime powder on the ground of the greenhouse, transferring the colored young silkworms into the greenhouse, and feeding the mulberry leaves for 3 times every day till the 3 rd instar;
step three, preparing a nutrient: mixing molasses, K2HPO4 inorganic salt and microorganism according to the weight ratio of 3:2:1, stirring, adding deionized water 2 times of the total amount of molasses, and stirring for 10-20 min;
step four, mulberry leaf treatment and feeding: spraying nutrient on folium Mori, and feeding colored silkworm until the stage of 5 years.
The preparation method of the treatment solution of this example was: and (2) placing 10 parts of vitamin into 30 parts of sodium chloride solution with the mass fraction of 20%, stirring for 20min, then adding 5 parts of pitaya peel extracting agent, stirring at the rotating speed of 200r/min for 20min, and obtaining the treatment solution after stirring.
The vitamin in this example is vitamin B7.
The preparation method of the pitaya peel extracting agent comprises the following steps:
s1: cleaning dragon fruit peel with clear water, adding deionized water 1-3 times of the weight of the dragon fruit peel, pulping, adding cellulase, stirring and performing enzymolysis for 4h to obtain an enzymolysis material;
s2: filtering and centrifuging the enzymolysis material, adding deionized water, boiling, filtering to obtain filtrate, and mixing the filtrates;
s3: adding the filter residue into 2 times of 80% alcohol solution by volume fraction, performing ultrasonic treatment, centrifuging, mixing all centrifugates, and mixing with the filtrate in S2 to obtain the pitaya peel extractant.
The temperature of stirring enzymolysis in this embodiment is 40 ℃, and the stirring rotation speed is 100 r/min.
The ultrasonic conditions in S3 of the present example were: 30kHz ultrasonic wave for 65 min.
The microorganism of this example was lactobacillus rhamnosus.
The inoculum size of the microorganism of this example was 4%.
Example 3:
the method for cultivating the colored silkworms in the greenhouse comprises the following steps:
firstly, primary rearing of color young silkworms: soaking folium Mori in the treating solution, performing illumination treatment with purple light with illumination intensity of 265Lx for 15min, and feeding color young silkworm with folium Mori until the young silkworm is 1.5 years old;
step two, building a greenhouse: building a greenhouse, scattering a layer of lime powder on the ground of the greenhouse, transferring the colored young silkworms into the greenhouse, and feeding the mulberry leaves for 3 times every day till the 3 rd instar;
step three, preparing a nutrient: mixing molasses, K2HPO4 inorganic salt and microorganism according to the weight ratio of 3:2:1, stirring, adding deionized water 2 times of the total amount of molasses, and stirring for 15 min;
step four, mulberry leaf treatment and feeding: spraying nutrient on folium Mori, and feeding colored silkworm until the stage of 5 years.
The preparation method of the treatment solution of this example was: and (2) placing 7.5 parts of vitamin into 25 parts of 15% sodium chloride solution by mass, stirring for 15min, then adding 3 parts of pitaya peel extracting agent, stirring for 15min at the rotating speed of 150r/min, and obtaining the treatment solution after stirring.
The vitamin of the embodiment is one or more of vitamin B11, vitamin B7, vitamin C and vitamin B1.
The preparation method of the pitaya peel extracting agent comprises the following steps:
s1: cleaning pitaya peel with clear water, adding deionized water which is 2 times of the weight of the pitaya peel, pulping, adding cellulase, stirring and performing enzymolysis for 3.5 hours to obtain an enzymolysis material;
s2: filtering and centrifuging the enzymolysis material, adding deionized water, boiling, filtering to obtain filtrate, and mixing the filtrates;
s3: adding the filter residue into 1.5 times of 77.5% alcohol solution by volume fraction, performing ultrasonic treatment, centrifuging, mixing all centrifugates, and mixing with the filtrate in S2 to obtain the pitaya peel extract.
In the embodiment, the temperature of stirring enzymolysis is 37.5 ℃, and the stirring speed is 75 r/min.
The ultrasonic conditions in S3 of the present example were: ultrasonic treatment at 20-30kHz for 55-65 min.
The microorganism of this example was Candida lipolytica.
The inoculation amount of the microorganism of this example was 3%.
Comparative example 1:
directly adopts mulberry leaves to breed silkworms.
The cultivation of silkworms was carried out according to examples 1 to 3 and comparative example 1, one per group of one-instar silkworms, the cultivation was carried out according to the method of the group, the cultivation process was observed and recorded, the cultivation time, the required weight of mulberry leaves and the weight of mature silkworms were recorded, and the results were as follows
Group of | Weight of silkworm (g) | Silk yield increase (%) |
Example 1 | 49.3 | 29.2 |
Example 2 | 49.5 | 30.1 |
Example 3 | 50.9 | 30.9 |
Comparative example 1 | 45.1 | 21.2 |
The examples 1-3 and the comparative example 1 show that the color silkworm breeding method can effectively improve the yield of the silk, and the yield improvement rate can reach 30.9%.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. A method for cultivating colored silkworms in a greenhouse is characterized by comprising the following steps:
firstly, primary rearing of color young silkworms: soaking mulberry leaves in a treatment solution, then carrying out illumination treatment for 10-20min by adopting purple light with illumination intensity of 260-270Lx, and feeding color young silkworms with the mulberry leaves until the color young silkworms reach 1-2 instars;
step two, building a greenhouse: building a greenhouse, scattering a layer of lime powder on the ground of the greenhouse, transferring the colored young silkworms into the greenhouse, and feeding the mulberry leaves for 2-3 times every day until the 3 rd instar;
step three, preparing a nutrient: mixing molasses, K2HPO4 inorganic salt and microorganism according to the weight ratio of 3:2:1, stirring, adding deionized water 2 times of the total amount of molasses, and stirring for 10-20 min;
step four, mulberry leaf treatment and feeding: spraying nutrient on folium Mori, and feeding colored silkworm until the age of colored silkworm is 4-5.
2. The method for cultivating greenhouse-cultivated colored silkworms according to claim 1, wherein the treatment solution is prepared by: 5-10 parts of vitamin is placed in 20-30 parts of 10-20% sodium chloride solution by mass for stirring for 10-20min, then 1-5 parts of pitaya peel extracting agent is added, and then stirring is carried out for 10-20min at the rotating speed of 100-200r/min, and the treatment fluid is obtained after the stirring is finished.
3. The method for cultivating the colored silkworms in the greenhouse as claimed in claim 2, wherein the vitamin is one or more of vitamin B11, vitamin B7, vitamin C and vitamin B1.
4. The method for cultivating greenhouse-cultivated colored silkworms according to claim 2, wherein the preparation method of the pitaya peel extractant comprises the following steps:
s1: cleaning dragon fruit peel with clear water, adding deionized water 1-3 times of dragon fruit peel weight, pulping, adding cellulase, stirring for enzymolysis for 3-4h to obtain enzymolysis material;
s2: filtering and centrifuging the enzymolysis material, adding deionized water, boiling, filtering to obtain filtrate, and mixing the filtrates;
s3: adding the filter residue into 1-2 times of 75-80% alcohol solution, ultrasonic treating, centrifuging, mixing all centrifugates, and mixing with the filtrate in S2 to obtain the dragon fruit peel extract.
5. The method for cultivating greenhouse-cultivated colored silkworms according to claim 4, wherein the temperature of stirring enzymolysis is 35-40 ℃ and the stirring rotation speed is 50-100 r/min.
6. The method for cultivating greenhouse-cultivated colored silkworms according to claim 5, wherein the temperature of stirring enzymolysis is 37.5 ℃ and the stirring rotation speed is 75 r/min.
7. The method for cultivating greenhouse-cultivated colored silkworms according to claim 4, wherein the ultrasonic conditions in S3 are as follows: ultrasonic treatment at 20-30kHz for 55-65 min.
8. The method for cultivating greenhouse-cultivated colored silkworms according to claim 1, wherein the microorganism is one of lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus fermentum, candida lipolytica, and wine yeast.
9. The method according to claim 1, wherein the amount of the microorganism is 2-4%.
10. The method according to claim 9, wherein the amount of the microorganism is 3%.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011584935.0A CN114680085A (en) | 2020-12-29 | 2020-12-29 | Method for cultivating colored silkworms in greenhouse |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202011584935.0A CN114680085A (en) | 2020-12-29 | 2020-12-29 | Method for cultivating colored silkworms in greenhouse |
Publications (1)
Publication Number | Publication Date |
---|---|
CN114680085A true CN114680085A (en) | 2022-07-01 |
Family
ID=82129903
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202011584935.0A Pending CN114680085A (en) | 2020-12-29 | 2020-12-29 | Method for cultivating colored silkworms in greenhouse |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114680085A (en) |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106804539A (en) * | 2016-11-30 | 2017-06-09 | 河池市金城江区科学技术情报研究所 | A kind of cooperative rearing of young silkworm method |
CN107258707A (en) * | 2017-07-15 | 2017-10-20 | 合肥市聚丰制丝有限责任公司 | A kind of cultural method for improving the silkworm amount of cocoon production and production cocoon quality amount |
CN107258706A (en) * | 2017-07-15 | 2017-10-20 | 合肥市聚丰制丝有限责任公司 | A kind of cultural method for improving the silkworm amount of cocooing |
CN109006716A (en) * | 2018-10-31 | 2018-12-18 | 十堰家楼真丝工贸有限公司 | A kind of cultural method improving Production of Bombyx Mori cocoon quality amount |
CN109160955A (en) * | 2018-11-05 | 2019-01-08 | 贺州学院 | The method of dragon fruit pericarp extraction polysaccharide |
CN109439492A (en) * | 2018-12-24 | 2019-03-08 | 广西壮族自治区农业科学院葡萄与葡萄酒研究所 | A kind of brewage process of BPH resistant rice variety red meat Pitaya wine |
CN109439497A (en) * | 2018-12-24 | 2019-03-08 | 广西壮族自治区农业科学院葡萄与葡萄酒研究所 | A kind of brewage process of dragon fruit sweet taste dry-type fruit wine |
-
2020
- 2020-12-29 CN CN202011584935.0A patent/CN114680085A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106804539A (en) * | 2016-11-30 | 2017-06-09 | 河池市金城江区科学技术情报研究所 | A kind of cooperative rearing of young silkworm method |
CN107258707A (en) * | 2017-07-15 | 2017-10-20 | 合肥市聚丰制丝有限责任公司 | A kind of cultural method for improving the silkworm amount of cocoon production and production cocoon quality amount |
CN107258706A (en) * | 2017-07-15 | 2017-10-20 | 合肥市聚丰制丝有限责任公司 | A kind of cultural method for improving the silkworm amount of cocooing |
CN109006716A (en) * | 2018-10-31 | 2018-12-18 | 十堰家楼真丝工贸有限公司 | A kind of cultural method improving Production of Bombyx Mori cocoon quality amount |
CN109160955A (en) * | 2018-11-05 | 2019-01-08 | 贺州学院 | The method of dragon fruit pericarp extraction polysaccharide |
CN109439492A (en) * | 2018-12-24 | 2019-03-08 | 广西壮族自治区农业科学院葡萄与葡萄酒研究所 | A kind of brewage process of BPH resistant rice variety red meat Pitaya wine |
CN109439497A (en) * | 2018-12-24 | 2019-03-08 | 广西壮族自治区农业科学院葡萄与葡萄酒研究所 | A kind of brewage process of dragon fruit sweet taste dry-type fruit wine |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102584430B (en) | Method for manufacturing amino-acid fishpond fertilizer water aqua by waste bave protein | |
KR100264675B1 (en) | Preparation method of natural indigo from polygoum tinctorlum | |
CN101491229A (en) | Vitro culturing method of pearl shell mantle tissue and culture medium | |
CN103478028A (en) | Directional cultivation method of white or purple fresh water pearl | |
CN102648704A (en) | Method for making mulberry silkworm spin glossy dark green silkworm cocoon | |
CN104372034B (en) | A kind of method that hairy roots of giant knotweed produces resveratrol and expansion culture | |
CN103598129A (en) | Method for cultivating iridescence fresh water pearls | |
CN114680085A (en) | Method for cultivating colored silkworms in greenhouse | |
CN102618486B (en) | Culture medium for culturing cell slices of blue pearls | |
DE1792362A1 (en) | Process for the production of β-amylase | |
CN109757377A (en) | A kind of cultural method for accelerating fritillaria thunbergii reproduction speed | |
CN102337220B (en) | Penicillium purpurogenum DB1 strain and preparation and application thereof | |
CN114214211A (en) | Golden flower bacterium-aspergillus kawachii strain and preparation method and application thereof | |
CN113141969A (en) | Method for cultivating ganoderma lucidum | |
CN1123327A (en) | Method for preparation of high colour-value water-soluble red pigment of red rice | |
CN108849658B (en) | Method for improving metamorphosis attachment rate of pinctada martensii eyespot larvae | |
CN102329737B (en) | Eupenicillium javanicum DB4 strain as well as preparation and application thereof | |
CN105463861A (en) | Biological flax roving degumming method | |
CN111296176A (en) | Preparation method of ganoderma lucidum nutrient solution and rapid ganoderma lucidum cultivation technology | |
CN115824757B (en) | Dyeing method for endophytic fungi of camellia oleifera root system | |
CN114108336B (en) | Preparation method of color bacterial cellulose mask | |
KR101689850B1 (en) | Manufacture method of indigo plant dyestuff and Dyeing method | |
CN108782375A (en) | The breeding method of nucleated pearl | |
CN116515331B (en) | Preparation and application methods of apple bagging-free film agent | |
CN114197226B (en) | Preparation method of colored bacterial cellulose |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220701 |
|
RJ01 | Rejection of invention patent application after publication |