CN107258706A - A kind of cultural method for improving the silkworm amount of cocooing - Google Patents
A kind of cultural method for improving the silkworm amount of cocooing Download PDFInfo
- Publication number
- CN107258706A CN107258706A CN201710577834.2A CN201710577834A CN107258706A CN 107258706 A CN107258706 A CN 107258706A CN 201710577834 A CN201710577834 A CN 201710577834A CN 107258706 A CN107258706 A CN 107258706A
- Authority
- CN
- China
- Prior art keywords
- silkworm
- mulberry leaf
- cocooing
- weight
- cultural method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 119
- 238000000034 method Methods 0.000 title claims abstract description 44
- 240000000249 Morus alba Species 0.000 claims abstract description 66
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 65
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 238000003306 harvesting Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 30
- 235000013717 Houttuynia Nutrition 0.000 claims description 25
- 238000001914 filtration Methods 0.000 claims description 22
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 18
- 241000894006 Bacteria Species 0.000 claims description 16
- 235000014655 lactic acid Nutrition 0.000 claims description 15
- 239000004310 lactic acid Substances 0.000 claims description 15
- 229940067866 dandelion extract Drugs 0.000 claims description 13
- 235000020691 dandelion extract Nutrition 0.000 claims description 13
- 239000001845 taraxacum officinale leaf extract Substances 0.000 claims description 13
- 229920002752 Konjac Polymers 0.000 claims description 12
- 229920002472 Starch Polymers 0.000 claims description 12
- 241000245665 Taraxacum Species 0.000 claims description 12
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 claims description 12
- 235000021552 granulated sugar Nutrition 0.000 claims description 12
- 238000005286 illumination Methods 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 235000019698 starch Nutrition 0.000 claims description 12
- 239000008107 starch Substances 0.000 claims description 12
- 238000002604 ultrasonography Methods 0.000 claims description 12
- 238000009987 spinning Methods 0.000 claims description 11
- -1 compound amino acid Chemical class 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 10
- 150000003839 salts Chemical class 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 9
- 241000235342 Saccharomycetes Species 0.000 claims description 9
- 229930003268 Vitamin C Natural products 0.000 claims description 9
- 230000001476 alcoholic effect Effects 0.000 claims description 9
- 235000010485 konjac Nutrition 0.000 claims description 9
- 235000016709 nutrition Nutrition 0.000 claims description 9
- 235000019154 vitamin C Nutrition 0.000 claims description 9
- 239000011718 vitamin C Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 8
- 239000003513 alkali Substances 0.000 claims description 6
- CDAWCLOXVUBKRW-UHFFFAOYSA-N 2-aminophenol Chemical compound NC1=CC=CC=C1O CDAWCLOXVUBKRW-UHFFFAOYSA-N 0.000 claims description 5
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 5
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 5
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 5
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 5
- 230000035764 nutrition Effects 0.000 claims description 4
- 240000000691 Houttuynia cordata Species 0.000 claims 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 238000009366 sericulture Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 34
- 241000758769 Houttuynia Species 0.000 description 20
- 238000012258 culturing Methods 0.000 description 8
- 210000004907 gland Anatomy 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000005059 dormancy Effects 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000004904 shortening Methods 0.000 description 5
- 150000003384 small molecules Chemical class 0.000 description 5
- 230000001360 synchronised effect Effects 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- 241000186660 Lactobacillus Species 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000003054 hormonal effect Effects 0.000 description 4
- 229940039696 lactobacillus Drugs 0.000 description 4
- 244000205754 Colocasia esculenta Species 0.000 description 3
- 235000006481 Colocasia esculenta Nutrition 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 239000004576 sand Substances 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 1
- 235000006721 Morus bombycis Nutrition 0.000 description 1
- 101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000009940 knitting Methods 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Fodder In General (AREA)
Abstract
The invention mainly relates to technical field of breeding silkworms, a kind of cultural method for improving the silkworm amount of cocooing is disclosed, including：Mulberry leaf harvesting, three to four age silkworms, fifth instar larva, cocoon；Method is simple, the amount of cocooing of every silkworm is reached 51.3kg, the filament length of silk cocoon makes the income of sericulture peasant household improve 15.4% up to 1988m；Fresh-keeping liquid is sprayed immediately after mulberry leaf harvesting to be stored, fresh keeping time is long, it is to avoid harvesting daily, use manpower and material resources sparingly, fresh-keeping liquid contains many kinds of substance, and obtains after being fermented through multiple-microorganism, and pH is 4.2 ~ 4.6, mulberry leaf can be kept light green, silkworm can be promoted to digest and assimilate mulberry leaf simultaneously, improve silkworm resistance against diseases, reduced silkworm medicine and use, residual is avoided, silk quality is improved.
Description
Technical field
The invention mainly relates to technical field of breeding silkworms, more particularly to a kind of cultural method for improving the silkworm amount of cocooing.
Background technology
Silk is a kind of natural fiber, is also that our people is elongated using one of earliest animal origin, silk light weight,
Fabric sheen is good, comfortable and easy to wear, and feel is smooth plentiful, poor heat conductivity, and moisture absorption is breathed freely, and for the various silks and satins of the system of knitting and knitwear, goes back
It can be used for industry, national defence and medicine and other fields.But in the cultural method of the current silkworm of China, but the cultivation of silkworm at present
In method, the time of cocooing of silkworm, the amount of cocooing of every silkworm was 35 ~ 45kg, and spinning amount otherness is big, cocoon for two days two nights
Filament length be 700 ~ 1500m, the income of sericulture peasant household has been had a strong impact on, accordingly, it would be desirable to which a kind of improve the silkworm amount of cocooing
Cultural method.
The content of the invention
In order to make up the defect of prior art, it is an object of the invention to provide a kind of cultivation side for improving the silkworm amount of cocooing
Method.
A kind of cultural method for improving the silkworm amount of cocooing, comprises the following steps：
（1）Mulberry leaf are harvested：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, and fresh-keeping liquid contains a variety of things
Matter, and obtain after being fermented through multiple-microorganism, mulberry leaf can be kept light green, while digestion of the silkworm to mulberry leaf can be promoted to inhale
Receive, improve silkworm resistance against diseases, reduce silkworm medicine and use, it is to avoid residual, improve silk quality, every 2 days harvesting mulberry leaf are once；
（2）Three to four age silkworms：After molting keeping temperature be 26 DEG C, ambient humidity be 75 ~ 77%, control light application time small for 14 ~ 15
When, intensity of illumination is 200 ~ 300lx, and daily with ultraviolet irradiation once, 320~400nm of wavelength, each irradiation time is 8 ~ 10
Minute, promote growing for domestic natural silk gland, accelerate dormancy and cast off a skin, shorten the culturing time of silkworm, improve cultivation efficiency, feeding
Nutrient solution uniformly is sprayed during mulberry leaf, rich in small molecule nutritional ingredient, promotes to absorb, accelerates the synthesis of sericterium internal protein, improve knot
Cocoon amount；
（3）Fifth instar larva：After molting keeping temperature be 27 DEG C, ambient humidity be 73 ~ 75%, it is 11 ~ 12 hours to control light application time,
Intensity of illumination is 150 ~ 200lx, and cultivation temperature is further improved, and is promoted sericterium growth, is shortened light application time, silkworm is adapted to knot
The illumination condition of cocoon phase, accelerates spinning and cocoons；
（4）Cocoon：The 5th day of five ages, edible alkali solution is uniformly sprayed to mulberry leaf, and mass concentration is 0.2 ~ 0.4%, makes silkworm
PH is raised, and adjusts the hormonal readiness of silkworm, is made silkworm synchronous ripe and is cocoond, shortening is cocoond the time, hence it is evident that improves supporting for silkworm
Efficiency is grown, fountain height carries out spinning and cocoond for matured silkworm is placed on cluster device after 0.3 ~ 0.5%, 20 ~ 24 hours of mulberry leaf weight.
The step（1）Fresh-keeping liquid, fountain height be mulberry leaf weight 1.2 ~ 1.6%, by the raw material system of following parts by weight
Into：White granulated sugar 12 ~ 14, lactic acid bacteria 2 ~ 4, saccharomycete 2 ~ 4, salt 1.2 ~ 1.4, vitamin C 0.03 ~ 0.05, by white granulated sugar and dimension
Raw element C is added to the water, and the mass concentration for making white granulated sugar is 6 ~ 8%, accesses lactic acid bacteria, is fermented 11 ~ 12 hours in 38 ~ 40 DEG C, access
Saccharomycete, ferments 28 ~ 30 hours in 22 ~ 24 DEG C, adds salt, stirs, obtain fresh-keeping liquid.
Described lactic acid bacteria, is made up of the bacterial strain of following parts by weight：Lactobacillus acidophilus 13 ~ 15, Lactobacillus plantarum 8 ~ 10, guarantor
Plus Leah lactobacillus 5 ~ 7.
The step（2）Nutrient solution, fountain height be mulberry leaf weight 0.6 ~ 0.8%, by the raw material group of following parts by weight
Into：Konjak starch 17 ~ 19, dandelion extract 6 ~ 8, houttuynia extract 6 ~ 8, compound amino acid 2 ~ 4, konjak starch is added
In water, the mass concentration for making konjak starch is 0.4 ~ 0.6%, heating fully gelatinization, is cooled to after room temperature, addition is remaining original,
Stir, obtain nutrient solution.
Described dandelion extract, after dandelion is crushed, adds the water of 80 ~ 100 times of amounts of dandelion weight, heating is boiled
It is original 1/10 to boil to volume, and filtering adds the boiling water of 40 ~ 50 times of amounts of filter residue weight, in 30 ~ 35 points of 33 ~ 35kHz ultrasounds
Clock, filtering merges all filtrates, and drying to water content is 10 ~ 12%, obtains dandelion extract.
Described houttuynia extract, after cordate houttuynia is crushed, the volume fraction for adding 60 ~ 70 times of amounts of cordate houttuynia weight is
77 ~ 79% alcoholic solution, in 31 ~ 33kHz ultrasounds 30 ~ 35 minutes, filtering added 40 ~ 50 times of amounts of filter residue weight into filter residue
Volume fraction is 35 ~ 37% alcoholic solution, and ultrasound 20 ~ 25 minutes, filtering adds the water of 40 ~ 50 times of amounts of filter residue weight, decocted
It is original 1/10 to volume, filtering merges all filtrates, and drying to water content is 8 ~ 10%, obtains houttuynia extract.
Described compound amino acid, is commercially available composite aminophenol powder.
The cultural method for improving the silkworm amount of cocooing, obtained silkworm.
The silkworm, spin the silk cocoon formed.
It is an advantage of the invention that：The cultural method for the raising silkworm amount of cocooing that the present invention is provided, method is simple, makes every family
The amount of cocooing of silkworm reaches 51.3kg, and the filament length of silk cocoon makes the income of sericulture peasant household improve 15.4% up to 1988m；Mulberry leaf are adopted
Fresh-keeping liquid is sprayed after receipts immediately to be stored, fresh keeping time is long, it is to avoid harvesting daily, used manpower and material resources sparingly, fresh-keeping liquid contains many
Kind of material, and obtain after being fermented through multiple-microorganism, pH is 4.2 ~ 4.6, mulberry leaf can be kept light green, while can promote silkworm
Mulberry leaf are digested and assimilated, silkworm resistance against diseases is improved, silkworm medicine is reduced and uses, it is to avoid residual, improve silk quality；Three to four ages
The cultivation temperature of silkworm is improved, and extends light application time, and carries out long wave ultraviolet light irradiation in short-term, ultraviolet light is entered silkworm cortex,
Promote growing for domestic natural silk gland, accelerate dormancy and cast off a skin, shorten the culturing time of silkworm, improve cultivation efficiency；Sprayed in mulberry leaf
Nutrient solution is spilt, rich in small molecule nutritional ingredient, promotes to absorb, accelerates the synthesis of sericterium internal protein, improve the amount of cocooing；Supported during five ages
Grow temperature further to improve, promote sericterium growth, shorten light application time, silkworm is adapted to the illumination condition of phase of cocooing, accelerate to tell
Silk is cocoond；Edible alkali solution is sprayed in the 5th day of five ages to mulberry leaf, the pH of silkworm is raised 0.2 ~ 0.4, adjusts the hormone water of silkworm
It is flat, make silkworm synchronous ripe and cocoon, make time shortening 1/4 of cocooing, hence it is evident that improve the cultivation efficiency of silkworm.
Embodiment
Illustrate the present invention with specific embodiment below.
Embodiment 1
A kind of cultural method for improving the silkworm amount of cocooing, comprises the following steps：
（1）Mulberry leaf are harvested：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, and fresh-keeping liquid contains a variety of things
Matter, and obtain after being fermented through multiple-microorganism, mulberry leaf can be kept light green, while digestion of the silkworm to mulberry leaf can be promoted to inhale
Receive, improve silkworm resistance against diseases, reduce silkworm medicine and use, it is to avoid residual, improve silk quality, every 2 days harvesting mulberry leaf are once；
（2）Three to four age silkworms：After molting keeping temperature be 26 DEG C, ambient humidity be 75%, it is 14 hours, light to control light application time
It is 200lx according to intensity, daily with ultraviolet irradiation once, wavelength 320nm, each irradiation time is 8 minutes, promotes domestic natural silk gland
Grow, accelerate dormancy cast off a skin, shorten silkworm culturing time, improve cultivation efficiency, uniformly spray battalion during feeding mulberry leaf
Nutrient solution, rich in small molecule nutritional ingredient, promotes to absorb, accelerates the synthesis of sericterium internal protein, improve the amount of cocooing；
（3）Fifth instar larva：After molting keeping temperature be 27 DEG C, ambient humidity be 73%, it is 11 hours to control light application time, and illumination is strong
Spend for 150lx, cultivation temperature is further improved, promote sericterium growth, shorten light application time, silkworm is adapted to cocoon the illumination of phase
Condition, accelerates spinning and cocoons；
（4）Cocoon：The 5th day of five ages, edible alkali solution is uniformly sprayed to mulberry leaf, and mass concentration is 0.2%, makes the pH liters of silkworm
Height, adjusts the hormonal readiness of silkworm, makes silkworm synchronous ripe and cocoons, shortening is cocoond the time, hence it is evident that improve the cultivation effect of silkworm
Rate, fountain height for mulberry leaf weight 0.3%, 20 ~ 24 hours after matured silkworm is placed on cluster device, carry out spinning cocoon.
The step（1）Fresh-keeping liquid, fountain height be mulberry leaf weight 1.2%, be made up of the raw material of following parts by weight：In vain
Granulated sugar 12, lactic acid bacteria 2, saccharomycete 2, salt 1.2, vitamin C 0.03, white granulated sugar and vitamin C are added to the water, make white sand
The mass concentration of sugar is 6%, accesses lactic acid bacteria, is fermented 11 hours in 38 DEG C, accesses saccharomycete, is fermented 28 hours in 22 DEG C, is added
Salt, stirs, and obtains fresh-keeping liquid.
Described lactic acid bacteria, is made up of the bacterial strain of following parts by weight：Lactobacillus acidophilus 13, Lactobacillus plantarum 8, Bulgaria
Lactobacillus 5.
The step（2）Nutrient solution, fountain height be mulberry leaf weight 0.6%, be made up of the raw material of following parts by weight：Evil spirit
Taro starch 17, dandelion extract 6, houttuynia extract 6, compound amino acid 2, konjak starch is added to the water, konjaku is formed sediment
The mass concentration of powder is 0.4%, heating fully gelatinization, is cooled to after room temperature, addition is remaining original, stirs, obtains nutrition
Liquid.
Described dandelion extract, after dandelion is crushed, adds the water of 80 times of amounts of dandelion weight, heating boil to
Volume is original 1/10, and filtering adds the boiling water of 40 times of amounts of filter residue weight, ultrasonic 30 minutes in 33kHz, and filtering merges institute
There is filtrate, drying to water content is 10 ~ 12%, obtains dandelion extract.
Described houttuynia extract, after cordate houttuynia is crushed, the volume fraction for adding 60 times of amounts of cordate houttuynia weight is 77%
Alcoholic solution, in 31kHz ultrasounds 30 minutes, filtering, the volume fraction that 40 times of amounts of filter residue weight are added into filter residue was 35%
Alcoholic solution, ultrasound 20 minutes, filtering adds the water of 40 times of amounts of filter residue weight, and it is original 1/10 to decoct to volume, filtering,
Merge all filtrates, drying to water content is 8 ~ 10%, obtains houttuynia extract.
Described compound amino acid, is commercially available composite aminophenol powder.
The cultural method for improving the silkworm amount of cocooing, obtained silkworm.
The silkworm, spin the silk cocoon formed.
Embodiment 2
A kind of cultural method for improving the silkworm amount of cocooing, comprises the following steps：
（1）Mulberry leaf are harvested：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, and fresh-keeping liquid contains a variety of things
Matter, and obtain after being fermented through multiple-microorganism, mulberry leaf can be kept light green, while digestion of the silkworm to mulberry leaf can be promoted to inhale
Receive, improve silkworm resistance against diseases, reduce silkworm medicine and use, it is to avoid residual, improve silk quality, every 2 days harvesting mulberry leaf are once；
（2）Three to four age silkworms：After molting keeping temperature be 26 DEG C, ambient humidity be 76%, it is 14 hours, light to control light application time
It is 250lx according to intensity, daily with ultraviolet irradiation once, wavelength 360nm, each irradiation time is 9 minutes, promotes domestic natural silk gland
Grow, accelerate dormancy cast off a skin, shorten silkworm culturing time, improve cultivation efficiency, uniformly spray battalion during feeding mulberry leaf
Nutrient solution, rich in small molecule nutritional ingredient, promotes to absorb, accelerates the synthesis of sericterium internal protein, improve the amount of cocooing；
（3）Fifth instar larva：After molting keeping temperature be 27 DEG C, ambient humidity be 74%, it is 11 hours to control light application time, and illumination is strong
Spend for 180lx, cultivation temperature is further improved, promote sericterium growth, shorten light application time, silkworm is adapted to cocoon the illumination of phase
Condition, accelerates spinning and cocoons；
（4）Cocoon：The 5th day of five ages, edible alkali solution is uniformly sprayed to mulberry leaf, and mass concentration is 0.3%, makes the pH liters of silkworm
Height, adjusts the hormonal readiness of silkworm, makes silkworm synchronous ripe and cocoons, shortening is cocoond the time, hence it is evident that improve the cultivation effect of silkworm
Rate, fountain height for mulberry leaf weight 0.4%, 20 ~ 24 hours after matured silkworm is placed on cluster device, carry out spinning cocoon.
The step（1）Fresh-keeping liquid, fountain height be mulberry leaf weight 1.4%, be made up of the raw material of following parts by weight：In vain
Granulated sugar 13, lactic acid bacteria 3, saccharomycete 3, salt 1.3, vitamin C 0.04, white granulated sugar and vitamin C are added to the water, make white sand
The mass concentration of sugar is 7%, accesses lactic acid bacteria, is fermented 11 hours in 39 DEG C, accesses saccharomycete, is fermented 29 hours in 23 DEG C, is added
Salt, stirs, and obtains fresh-keeping liquid.
Described lactic acid bacteria, is made up of the bacterial strain of following parts by weight：Lactobacillus acidophilus 14, Lactobacillus plantarum 9, Bulgaria
Lactobacillus 6.
The step（2）Nutrient solution, fountain height be mulberry leaf weight 0.7%, be made up of the raw material of following parts by weight：Evil spirit
Taro starch 18, dandelion extract 7, houttuynia extract 7, compound amino acid 3, konjak starch is added to the water, konjaku is formed sediment
The mass concentration of powder is 0.5%, heating fully gelatinization, is cooled to after room temperature, addition is remaining original, stirs, obtains nutrition
Liquid.
Described dandelion extract, after dandelion is crushed, adds the water of 90 times of amounts of dandelion weight, heating boil to
Volume is original 1/10, and filtering adds the boiling water of 45 times of amounts of filter residue weight, ultrasonic 33 minutes in 34kHz, and filtering merges institute
There is filtrate, drying to water content is 10 ~ 12%, obtains dandelion extract.
Described houttuynia extract, after cordate houttuynia is crushed, the volume fraction for adding 65 times of amounts of cordate houttuynia weight is 78%
Alcoholic solution, in 32kHz ultrasounds 33 minutes, filtering, the volume fraction that 45 times of amounts of filter residue weight are added into filter residue was 36%
Alcoholic solution, ultrasound 23 minutes, filtering adds the water of 45 times of amounts of filter residue weight, and it is original 1/10 to decoct to volume, filtering,
Merge all filtrates, drying to water content is 8 ~ 10%, obtains houttuynia extract.
Described compound amino acid, is commercially available composite aminophenol powder.
The cultural method for improving the silkworm amount of cocooing, obtained silkworm.
The silkworm, spin the silk cocoon formed.
Embodiment 3
A kind of cultural method for improving the silkworm amount of cocooing, comprises the following steps：
（1）Mulberry leaf are harvested：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, and fresh-keeping liquid contains a variety of things
Matter, and obtain after being fermented through multiple-microorganism, mulberry leaf can be kept light green, while digestion of the silkworm to mulberry leaf can be promoted to inhale
Receive, improve silkworm resistance against diseases, reduce silkworm medicine and use, it is to avoid residual, improve silk quality, every 2 days harvesting mulberry leaf are once；
（2）Three to four age silkworms：After molting keeping temperature be 26 DEG C, ambient humidity be 77%, it is 15 hours, light to control light application time
It is 300lx according to intensity, daily with ultraviolet irradiation once, wavelength 400nm, each irradiation time is 10 minutes, promotes domestic silkworm silk
Growing for gland, accelerates dormancy and casts off a skin, shorten the culturing time of silkworm, improves cultivation efficiency, is uniformly sprayed during feeding mulberry leaf
Nutrient solution, rich in small molecule nutritional ingredient, promotes to absorb, accelerates the synthesis of sericterium internal protein, improve the amount of cocooing；
（3）Fifth instar larva：After molting keeping temperature be 27 DEG C, ambient humidity be 75%, it is 12 hours to control light application time, and illumination is strong
Spend for 200lx, cultivation temperature is further improved, promote sericterium growth, shorten light application time, silkworm is adapted to cocoon the illumination of phase
Condition, accelerates spinning and cocoons；
（4）Cocoon：The 5th day of five ages, edible alkali solution is uniformly sprayed to mulberry leaf, and mass concentration is 0.4%, makes the pH liters of silkworm
Height, adjusts the hormonal readiness of silkworm, makes silkworm synchronous ripe and cocoons, shortening is cocoond the time, hence it is evident that improve the cultivation effect of silkworm
Rate, fountain height for mulberry leaf weight 0.5%, 20 ~ 24 hours after matured silkworm is placed on cluster device, carry out spinning cocoon.
The step（1）Fresh-keeping liquid, fountain height be mulberry leaf weight 1.6%, be made up of the raw material of following parts by weight：In vain
Granulated sugar 14, lactic acid bacteria 4, saccharomycete 4, salt 1.4, vitamin C 0.05, white granulated sugar and vitamin C are added to the water, make white sand
The mass concentration of sugar is 8%, accesses lactic acid bacteria, is fermented 12 hours in 40 DEG C, accesses saccharomycete, is fermented 30 hours in 24 DEG C, is added
Salt, stirs, and obtains fresh-keeping liquid.
Described lactic acid bacteria, is made up of the bacterial strain of following parts by weight：Lactobacillus acidophilus 15, Lactobacillus plantarum 10, Bao Jiali
Sub- lactobacillus 7.
The step（2）Nutrient solution, fountain height be mulberry leaf weight 0.8%, be made up of the raw material of following parts by weight：Evil spirit
Taro starch 19, dandelion extract 8, houttuynia extract 8, compound amino acid 4, konjak starch is added to the water, konjaku is formed sediment
The mass concentration of powder is 0.6%, heating fully gelatinization, is cooled to after room temperature, addition is remaining original, stirs, obtains nutrition
Liquid.
Described dandelion extract, after dandelion is crushed, adds the water of 100 times of amounts of dandelion weight, heating is boiled
It is original 1/10 to volume, filtering adds the boiling water of 50 times of amounts of filter residue weight, ultrasonic 35 minutes in 35kHz, filters, and merges
All filtrates, drying to water content is 10 ~ 12%, obtains dandelion extract.
Described houttuynia extract, after cordate houttuynia is crushed, the volume fraction for adding 70 times of amounts of cordate houttuynia weight is 79%
Alcoholic solution, in 33kHz ultrasounds 35 minutes, filtering, the volume fraction that 50 times of amounts of filter residue weight are added into filter residue was 37%
Alcoholic solution, ultrasound 25 minutes, filtering adds the water of 50 times of amounts of filter residue weight, and it is original 1/10 to decoct to volume, filtering,
Merge all filtrates, drying to water content is 8 ~ 10%, obtains houttuynia extract.
Described compound amino acid, is commercially available composite aminophenol powder.
The cultural method for improving the silkworm amount of cocooing, obtained silkworm.
The silkworm, spin the silk cocoon formed.
Comparative example 1
Removal step（1）In fresh-keeping liquid, remaining method, be the same as Example 1.
Comparative example 2
Step（2）In temperature be 24 DEG C, remaining method, be the same as Example 1.
Comparative example 3
Removal step（2）In ultraviolet light, remaining method, be the same as Example 1.
Comparative example 4
Step（2）In be natural lighting, remaining method, be the same as Example 1.
Comparative example 5
Step（3）In temperature be 25 DEG C, remaining method, be the same as Example 1.
Comparative example 6
Step（3）In be natural lighting, remaining method, be the same as Example 1.
Comparative example 7
Removal step（4）, remaining method, be the same as Example 1.
Comparative example 8
Traditional cultural method of existing silkworm.
The culture efficiency of embodiment and comparative example silkworm cultural method：
The cultivation of silkworm is carried out according to the method for each group respectively, every group of age silkworm one is supported according to the method for the group respectively
Grow, observe and record breeding process, record culturing time, required mulberry leaf weight and matured silkworm body weight the results are shown in Table 1, during five ages
The matured silkworm 10 of each group is randomly choosed, according to the method for " measure of Pancreas Glutamate-Pyruvate Transaminase Vigor in several tissues of silkworm, China just etc. ",
Detect the silk cocoon each 10 of the protein content of each group matured silkworm posterior division of silkgland and middle division of silkgland, random selection embodiment and comparative example
Individual, after the silk cocoon of each group is cooked one by one, abundant dryness in the sun to water content is 0 ~ 2%, and silk is flat contained by detection each group silk cocoon
Equal length and average diameter, the results are shown in Table 2.
Table 1：Embodiment and the cultivation situation of comparative example silkworm
| Project | Culturing time/（d） | Mulberry leaf weight/（kg） | Matured silkworm body weight/（G/10 fresh weights） |
| Embodiment 1 | 24 | 662 | 46.4 |
| Embodiment 2 | 24 | 667 | 46.3 |
| Embodiment 3 | 24 | 663 | 46.6 |
| Comparative example 1 | 25 | 685 | 47.3 |
| Comparative example 2 | 25 | 688 | 48.5 |
| Comparative example 3 | 26 | 684 | 47.4 |
| Comparative example 4 | 26 | 685 | 48.3 |
| Comparative example 5 | 26 | 696 | 48.8 |
| Comparative example 6 | 26 | 715 | 48.1 |
| Comparative example 7 | 26 | 722 | 48.6 |
| Comparative example 8 | 26 | 784 | 50.3 |
Show from the result of table 1, the cultural method of the raising silkworm amount of cocooing of embodiment, culturing time is substantially short compared with comparative example,
The mulberry leaf weight used is substantially few compared with comparative example, and matured silkworm body weight is slightly smaller compared with comparative example, illustrates the raising silkworm knot that the present invention is provided
The cultural method of cocoon amount has good cultivation side effect.
Table 2：The feature of embodiment and comparative example silk
| Project | Posterior division of silkgland/（mg/g） | Middle division of silkgland/（mg/g） | Length/（m） | Cocoon/（Kg/） |
| Embodiment 1 | 18.2 | 4.5 | 1976 | 50.8 |
| Embodiment 2 | 18.4 | 4.9 | 1988 | 51.3 |
| Embodiment 3 | 18.3 | 4.7 | 1972 | 50.7 |
| Comparative example 1 | 14.6 | 3.7 | 1265 | 41.8 |
| Comparative example 2 | 15.3 | 3.6 | 1384 | 38.1 |
| Comparative example 3 | 15.2 | 3.2 | 1253 | 38.8 |
| Comparative example 4 | 15.7 | 3.4 | 1236 | 38.5 |
| Comparative example 5 | 14.3 | 3.3 | 1276 | 39.4 |
| Comparative example 6 | 15.4 | 3.6 | 1295 | 38.2 |
| Comparative example 7 | 15.6 | 3.7 | 1287 | 39.7 |
| Comparative example 8 | 11.7 | 3.1 | 1114 | 37.9 |
Show from the result of table 2, the cultural method of the raising silkworm amount of cocooing of embodiment, make the posterior division of silkgland and middle part silk of matured silkworm
The protein content of gland is substantially high compared with comparative example, and the silk length contained of single silk cocoon is substantially big compared with comparative example, every silkworm
The amount of cocooing is substantially high compared with comparative example, illustrates the cultural method for the raising silkworm amount of cocooing that the present invention is provided in the same of increase spinning amount
When can also save mulberry leaf consumption, with preferable culture efficiency.
Claims (9)
1. a kind of cultural method for improving the silkworm amount of cocooing, it is characterised in that comprise the following steps：
（1）Mulberry leaf are harvested：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, every 2 days harvesting mulberry leaf one
It is secondary；
（2）Three to four age silkworms：After molting keeping temperature be 26 DEG C, ambient humidity be 75 ~ 77%, control light application time small for 14 ~ 15
When, intensity of illumination is 200 ~ 300lx, and daily with ultraviolet irradiation once, 320~400nm of wavelength, each irradiation time is 8 ~ 10
Minute, uniformly spray nutrient solution during feeding mulberry leaf；
（3）Fifth instar larva：After molting keeping temperature be 27 DEG C, ambient humidity be 73 ~ 75%, it is 11 ~ 12 hours to control light application time,
Intensity of illumination is 150 ~ 200lx；
（4）Cocoon：The 5th day of five ages, edible alkali solution is uniformly sprayed to mulberry leaf, and mass concentration is 0.2 ~ 0.4%, and fountain height is
Matured silkworm, is placed on cluster device by the 0.3 ~ 0.5% of mulberry leaf weight after 20 ~ 24 hours, carries out spinning and cocoons.
2. the cultural method of the silkworm amount of cocooing is improved according to claim 1, it is characterised in that the step（1）It is fresh-keeping
Liquid, fountain height is the 1.2 ~ 1.6% of mulberry leaf weight, is made up of the raw material of following parts by weight：White granulated sugar 12 ~ 14, lactic acid bacteria 2 ~ 4, ferment
Female bacterium 2 ~ 4, salt 1.2 ~ 1.4, vitamin C 0.03 ~ 0.05, white granulated sugar and vitamin C are added to the water, and make the matter of white granulated sugar
Amount concentration is 6 ~ 8%, accesses lactic acid bacteria, is fermented 11 ~ 12 hours in 38 ~ 40 DEG C, accesses saccharomycete, in 22 ~ 24 DEG C of fermentations 28 ~ 30
Hour, salt is added, stirs, obtains fresh-keeping liquid.
3. according to claim 2 improve the silkworm amount of cocooing cultural method, it is characterised in that described lactic acid bacteria, by with
The bacterial strain composition of lower parts by weight：Lactobacillus acidophilus 13 ~ 15, Lactobacillus plantarum 8 ~ 10, lactobacillus bulgaricus 5 ~ 7.
4. the cultural method of the silkworm amount of cocooing is improved according to claim 1, it is characterised in that the step（2）Nutrition
Liquid, fountain height is the 0.6 ~ 0.8% of mulberry leaf weight, is made up of the raw material of following parts by weight：Konjak starch 17 ~ 19, dandelion are extracted
Thing 6 ~ 8, houttuynia extract 6 ~ 8, compound amino acid 2 ~ 4, konjak starch is added to the water, and the mass concentration for making konjak starch is
0.4 ~ 0.6%, heating fully gelatinization is cooled to after room temperature, addition is remaining original, stirs, obtains nutrient solution.
5. the cultural method of the silkworm amount of cocooing is improved according to claim 4, it is characterised in that described dandelion is extracted
Thing, after dandelion is crushed, adds the water of 80 ~ 100 times of amounts of dandelion weight, it is original 1/10, mistake that heating, which is boiled to volume,
Filter, adds the boiling water of 40 ~ 50 times of amounts of filter residue weight, and in 33 ~ 35kHz ultrasounds 30 ~ 35 minutes, filtering merged all filtrates, drying
It is 10 ~ 12% to water content, obtains dandelion extract.
6. the cultural method of the silkworm amount of cocooing is improved according to claim 4, it is characterised in that described cordate houttuynia is extracted
Thing, after cordate houttuynia is crushed, the volume fraction for adding 60 ~ 70 times of amounts of cordate houttuynia weight is 77 ~ 79% alcoholic solution, in 31 ~
33kHz ultrasounds 30 ~ 35 minutes, filtering, the volume fraction that 40 ~ 50 times of amounts of filter residue weight are added into filter residue is 35 ~ 37% alcohol
Solution, ultrasound 20 ~ 25 minutes, filtering adds the water of 40 ~ 50 times of amounts of filter residue weight, and it is original 1/10, mistake to decoct to volume
Filter, merges all filtrates, and drying to water content is 8 ~ 10%, obtains houttuynia extract.
7. the cultural method of the silkworm amount of cocooing is improved according to claim 4, it is characterised in that described compound amino acid,
For commercially available composite aminophenol powder.
8. a kind of any one of claim 1 ~ 7 cultural method for improving the silkworm amount of cocooing, obtained silkworm.
9. silkworm described in a kind of claim 8, spin the silk cocoon formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710577834.2A CN107258706A (en) | 2017-07-15 | 2017-07-15 | A kind of cultural method for improving the silkworm amount of cocooing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710577834.2A CN107258706A (en) | 2017-07-15 | 2017-07-15 | A kind of cultural method for improving the silkworm amount of cocooing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107258706A true CN107258706A (en) | 2017-10-20 |
Family
ID=60072120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710577834.2A Pending CN107258706A (en) | 2017-07-15 | 2017-07-15 | A kind of cultural method for improving the silkworm amount of cocooing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107258706A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077197A (en) * | 2018-02-07 | 2018-05-29 | 四川红旗丝绸有限公司 | A kind of method of young silkworm farm breeds |
| CN110330557A (en) * | 2019-06-25 | 2019-10-15 | 浙江大学 | A method of improving silkworm seed weight |
| CN114680085A (en) * | 2020-12-29 | 2022-07-01 | 肥西吉新蚕桑专业合作社 | Method for cultivating colored silkworms in greenhouse |
| CN114680084A (en) * | 2020-12-29 | 2022-07-01 | 肥西吉新蚕桑专业合作社 | Breeding method for improving activity and health of young silkworms |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1931007A (en) * | 2006-10-09 | 2007-03-21 | 杨琦慧 | Silkworm feed additive |
| CN104509496A (en) * | 2014-11-28 | 2015-04-15 | 太和县金秋农民养殖专业合作社 | Silkworm breeding method |
| CN104543490A (en) * | 2014-12-18 | 2015-04-29 | 广西大学 | Additive for young silkworm feed and using method thereof |
| CN106306611A (en) * | 2016-08-29 | 2017-01-11 | 徐彬 | Supplementary nutritive feed for silkworms |
-
2017
- 2017-07-15 CN CN201710577834.2A patent/CN107258706A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1931007A (en) * | 2006-10-09 | 2007-03-21 | 杨琦慧 | Silkworm feed additive |
| CN104509496A (en) * | 2014-11-28 | 2015-04-15 | 太和县金秋农民养殖专业合作社 | Silkworm breeding method |
| CN104543490A (en) * | 2014-12-18 | 2015-04-29 | 广西大学 | Additive for young silkworm feed and using method thereof |
| CN106306611A (en) * | 2016-08-29 | 2017-01-11 | 徐彬 | Supplementary nutritive feed for silkworms |
Non-Patent Citations (1)
| Title |
|---|
| 张海玲等: "浅谈家蚕室内饲养", 《农技服务》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077197A (en) * | 2018-02-07 | 2018-05-29 | 四川红旗丝绸有限公司 | A kind of method of young silkworm farm breeds |
| CN110330557A (en) * | 2019-06-25 | 2019-10-15 | 浙江大学 | A method of improving silkworm seed weight |
| CN114680085A (en) * | 2020-12-29 | 2022-07-01 | 肥西吉新蚕桑专业合作社 | Method for cultivating colored silkworms in greenhouse |
| CN114680084A (en) * | 2020-12-29 | 2022-07-01 | 肥西吉新蚕桑专业合作社 | Breeding method for improving activity and health of young silkworms |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103815135B (en) | A kind of mulberry leaf fermented feed batching and production method thereof and purposes | |
| CN105002116B (en) | A kind of microbial straw feed microbial inoculum and preparation method thereof | |
| CN107258706A (en) | A kind of cultural method for improving the silkworm amount of cocooing | |
| CN110150458A (en) | A method of forage protein is prepared using microbial fermentation black soldier flies larva | |
| CN104542461A (en) | Breeding method for Laiwu pigs | |
| CN106615806A (en) | Biologically fermented feed for improving pork quality and production method and application of biologically fermented feed | |
| CN107373022B (en) | Pig feed | |
| CN101449706A (en) | Lily corn lactobacillus milk beverage and production method thereof | |
| CN106417915A (en) | Bream parent fish feed additive | |
| CN105053602A (en) | Mulberry pomace chicken feed and preparation method thereof | |
| CN105707442A (en) | Method for preparing protein feed and protein feed additive by culturing fly maggots with defined medium | |
| CN106615611A (en) | Method for manufacturing rice straw fermented feed | |
| CN110353087A (en) | The preparation method and fermented feed of rape stalk fermented feed | |
| CN106983042A (en) | A kind of pool cowfish feed and preparation method thereof | |
| CN106721674A (en) | A kind of feed of grass carp for adding fermented silkworms and fermentation silkworm excrement and application thereof | |
| CN108575885A (en) | A kind of black swine rearing method | |
| CN103098983B (en) | Method for producing straw biological feed | |
| CN108497183A (en) | A kind of black pig fermented feed and preparation method thereof | |
| CN106718042A (en) | A kind of method of utilization bamboo rat excrement Xinbao mushroom culturing | |
| CN107279074B (en) | Method for increasing silk spinning amount of mulberry silkworms | |
| CN109496981A (en) | A method of for improving Egg Production of Laying Hens quality | |
| CN107279523A (en) | Kind horse feed and preparation method thereof | |
| CN107333722A (en) | A kind of silkworm cultural method for saving mulberry leaf | |
| CN112772778A (en) | Feed and preparation method and application thereof | |
| CN105053529A (en) | Feeding bean material applicable to young Oreochromis mossambicus and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171020 |
|
| RJ01 | Rejection of invention patent application after publication |