CN107333722A - A kind of silkworm cultural method for saving mulberry leaf - Google Patents
A kind of silkworm cultural method for saving mulberry leaf Download PDFInfo
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- 240000000249 Morus alba Species 0.000 title claims abstract description 178
- 235000008708 Morus alba Nutrition 0.000 title claims abstract description 178
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 125
- 238000000034 method Methods 0.000 title claims abstract description 46
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 15
- 238000003306 harvesting Methods 0.000 claims abstract description 15
- 238000000855 fermentation Methods 0.000 claims abstract description 3
- 230000004151 fermentation Effects 0.000 claims abstract description 3
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 30
- 230000035611 feeding Effects 0.000 claims description 24
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 18
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- 235000014655 lactic acid Nutrition 0.000 claims description 15
- 239000004310 lactic acid Substances 0.000 claims description 15
- 235000021552 granulated sugar Nutrition 0.000 claims description 12
- 150000003839 salts Chemical class 0.000 claims description 10
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 9
- 229930003268 Vitamin C Natural products 0.000 claims description 9
- 235000019154 vitamin C Nutrition 0.000 claims description 9
- 239000011718 vitamin C Substances 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 5
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 5
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 10
- 239000003814 drug Substances 0.000 abstract description 7
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- 230000001488 breeding effect Effects 0.000 abstract description 6
- 229940079593 drug Drugs 0.000 abstract description 6
- 235000008935 nutritious Nutrition 0.000 abstract description 6
- 238000012258 culturing Methods 0.000 abstract description 5
- 239000002207 metabolite Substances 0.000 abstract description 5
- 238000009366 sericulture Methods 0.000 abstract description 2
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- 101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
- A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
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- A23V2400/169—Plantarum
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Abstract
The invention mainly relates to sericulture technical field, discloses a kind of silkworm cultural method for saving mulberry leaf, including：Mulberry leaf harvesting, an age silkworm, two to four age silkworms, fifth instar larva；Method is simple, and whole breeding process saves mulberry leaf 17 ~ 19%, shortens culturing time, and whole breeding process is 24 days, hence it is evident that improves breeding efficiency, income is improved 17.3%；Fresh-keeping liquid is sprayed after mulberry leaf harvesting, is stored, avoids harvesting mulberry leaf daily, labour is saved, fresh-keeping liquid adds Multiple components, formed by a variety of strain fermentations, it is nutritious, pH is 4.6 ~ 4.8, mulberry leaf can be kept bud green, while metabolite can promote silkworm to grow caused by saccharomycete and lactobacillus-fermented, strengthen resistance against diseases, mulberry leaf are saved, reduce silkworm medication, improve silk quality.
Description
Technical field
The invention mainly relates to sericulture technical field, more particularly to a kind of silkworm cultural method for saving mulberry leaf.
Background technology
Silk is a kind of natural fiber, and our people, using one of earliest animal origin, silk is light and elongated,
Fabric sheen is good, comfortable and easy to wear, and feel is smooth plentiful, and poor heat conductivity, moisture absorption is breathed freely, and for the various silks and satins of the system of knitting and knitwear, goes back
It can be used for industry, national defence and medicine and other fields.But in the cultural method of the current silkworm in China, the mulberry leaf amount of a silkworm needs
For 750~800kg, existing a small number of methods for saving mulberry leaf, it is about 50kg that every silkworm, which saves mulberry leaf amount, but 700 ~ 750kg
Mulberry leaf still need more man power and material, aquaculture cost is higher.
The content of the invention
The defects of in order to make up prior art, it is an object of the invention to provide a kind of silkworm cultural method for saving mulberry leaf.
A kind of silkworm cultural method for saving mulberry leaf, comprises the following steps：
（1）Mulberry leaf harvest：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, nutritious, pH be 4.6 ~
4.8, promote silkworm to digest and assimilate mulberry leaf, mulberry leaf can be kept bud green, while generation caused by saccharomycete and lactobacillus-fermented
Thanking to product can promote silkworm to grow, and strengthen resistance against diseases, save mulberry leaf, reduce silkworm medication, improve silk quality, harvesting amount
To be available for the silkworm amount of 2 ~ 3 days；
（2）One age silkworm：The ambient humidity for keeping silkworm room is 76 ~ 78%, and fresh mulberry is added again after removing residual mulberry leaf every morning
Leaf, the enough mulberry leaf of daily feeding, promote growth, improve resistance against diseases；
（3）Two to four age silkworms：Keeping temperature is 26 DEG C, ambient humidity is 74 ~ 76%, and it is 14 ~ 15 hours to control light application time, early
Upper and noon respectively adds mulberry leaf once, and morning, addition was the 35 ~ 40% of whole day mulberry leaf amount, and remaining mulberry leaf are added at noon
Add, improve temperature, extend illumination, promote the sericterium of silkworm ripe, shorten the growth time of each age silkworm, save mulberry leaf addition, enter
Stop addition mulberry leaf within 18 ~ 20 hours before dormancy, accelerate to fall asleep；
（4）Fifth instar larva：12 ~ 14 hours first feedings after molting, keeping temperature is 27 DEG C, ambient humidity is 73 ~ 75%, when controlling illumination
Between be 11 ~ 12 hours, mulberry leaf addition is the 35 ~ 40% of whole day mulberry leaf amount morning, and morning, mulberry leaf addition is whole day mulberry leaf amount
30 ~ 35%, remaining mulberry leaf are added at dusk, the growth of promotion sericterium and ripe, five times in age of shortening, daily on a small quantity repeatedly
Feeding mulberry leaf, feeding are sufficient, shorten light application time, and silkworm can be made to cocoon concentrations, be easy to be managed collectively, saving manpower and thing
Power.
The step（1）Fresh-keeping liquid, fountain height be mulberry leaf weight 1.2 ~ 1.6%, by the raw material system of following parts by weight
Into：White granulated sugar 12 ~ 14, lactic acid bacteria 2 ~ 4, saccharomycete 2 ~ 4, salt 1.2 ~ 1.4, vitamin C 0.03 ~ 0.05, by white granulated sugar and dimension
Raw plain C is added to the water, and the mass concentration for making white granulated sugar is 6 ~ 8%, accesses lactic acid bacteria, is fermented 11 ~ 12 hours in 38 ~ 40 DEG C, access
Saccharomycete, fermented 28 ~ 30 hours in 22 ~ 24 DEG C, add salt, stir, obtain fresh-keeping liquid.
Described lactic acid bacteria, it is made up of the bacterial strain of following parts by weight：Lactobacillus acidophilus 13 ~ 15, Lactobacillus plantarum 8 ~ 10, protect
Add Leah lactobacillus 5 ~ 7.
The step（2）New fresh mulberry leaf, each addition be an age silkworm eaten at 8 ~ 10 hours.
The step（3）Whole day mulberry leaf amount, the full mulberry leaf weight of two age silkworms is 1.0 ~ 1.2kg, the full mulberry leaf weight of three age silkworms
For 3.2 ~ 3.6kg, the full mulberry leaf weight of four age silkworms is 11 ~ 13kg.
The step（4）Whole day mulberry leaf amount, weight is 94 ~ 96kg.
The silkworm cultural method for saving mulberry leaf, obtained silkworm.
The silkworm, spin the silk cocoon formed.
It is an advantage of the invention that：The silkworm cultural method provided by the invention for saving mulberry leaf, method is simple, entirely cultivates
Cheng Jieyue mulberry leaf 17 ~ 19%, shorten culturing time, and whole breeding process is 24 days, hence it is evident that improves breeding efficiency, makes income
Improve 17.3%；Fresh-keeping liquid is sprayed after mulberry leaf harvesting, is stored, avoids harvesting mulberry leaf daily, saves labour, fresh-keeping liquid adds
Enter Multiple components, formed by a variety of strain fermentations, nutritious, pH is 4.6 ~ 4.8, promotes silkworm to digest and assimilate mulberry leaf,
Mulberry leaf can be kept bud green, while metabolite can promote silkworm to grow caused by saccharomycete and lactobacillus-fermented, enhancing is anti-
Sick ability, mulberry leaf are saved, reduce silkworm medication, improve silk quality；The enough mulberry leaf of the one daily feeding of age silkworm, promote growth,
Improve resistance against diseases；Two to four age silkworms improve temperature per 2 feeding mulberry leaf of natural gift, extend illumination, promote the sericterium of silkworm ripe,
Shorten the growth time of each age silkworm, save mulberry leaf addition, and stop mulberry leaf addition before falling asleep in advance, accelerate to fall asleep；Five ages
The temperature of silkworm further raises, and promotes sericterium growth and ripe, five times in age of shortening, repeatedly feeding mulberry leaf a small amount of daily, feeding
It is sufficient, shorten light application time, silkworm can be made to cocoon concentrations, be easy to be managed collectively, saving man power and material.
Embodiment
Illustrate the present invention with specific embodiment below.
Embodiment 1
A kind of silkworm cultural method for saving mulberry leaf, comprises the following steps：
（1）Mulberry leaf harvest：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, nutritious, pH be 4.6 ~
4.8, mulberry leaf can be kept bud green, while metabolite can promote silkworm to grow caused by saccharomycete and lactobacillus-fermented, increase
Strong resistance against diseases, mulberry leaf are saved, reduce silkworm medication, improve silk quality, harvesting amount is to be available for the silkworm amount of 2 ~ 3 days；
（2）One age silkworm：The ambient humidity for keeping silkworm room is 76%, adds new fresh mulberry leaf again after removing residual mulberry leaf every morning, often
The enough mulberry leaf of its feeding, promote growth, improve resistance against diseases；
（3）Two to four age silkworms：Keeping temperature is 26 DEG C, ambient humidity 74%, and it is 14 hours to control light application time, and morning is with
Noon respectively adds mulberry leaf once, and morning, addition was the 35% of whole day mulberry leaf amount, and remaining mulberry leaf are added at noon, improved temperature
Degree, extend illumination, promote the sericterium of silkworm ripe, shorten the growth time of each age silkworm, save mulberry leaf addition, fall asleep first 18 hours
Stop addition mulberry leaf, accelerate to fall asleep；
（4）Fifth instar larva：12 hours first feedings after molting, keeping temperature is 27 DEG C, ambient humidity 73%, and it is 11 to control light application time
Hour, morning, mulberry leaf addition was the 35% of whole day mulberry leaf amount, and morning, mulberry leaf addition was the 30% of whole day mulberry leaf amount, remaining mulberry
Leaf is added at dusk, promotes sericterium growth and ripe, five times in age of shortening, and a small amount of daily repeatedly feeding mulberry leaf, feeding fill
Foot, shorten light application time, silkworm can be made to cocoon concentrations, be easy to be managed collectively, saving man power and material.
The step（1）Fresh-keeping liquid, fountain height be mulberry leaf weight 1.2%, be made up of the raw material of following parts by weight：In vain
Granulated sugar 12, lactic acid bacteria 2, saccharomycete 2, salt 1.2, vitamin C 0.03, white granulated sugar and vitamin C are added to the water, make white sand
The mass concentration of sugar is 6%, accesses lactic acid bacteria, is fermented 11 hours in 38 DEG C, accesses saccharomycete, is fermented 28 hours in 22 DEG C, is added
Salt, stir, obtain fresh-keeping liquid.
Described lactic acid bacteria, it is made up of the bacterial strain of following parts by weight：Lactobacillus acidophilus 13, Lactobacillus plantarum 8, Bulgaria
Lactobacillus 5.
The step（2）New fresh mulberry leaf, each addition be an age silkworm eaten at 8 ~ 10 hours.
The step（3）Whole day mulberry leaf amount, the full mulberry leaf weight of two age silkworms is 1.0 ~ 1.2kg, the full mulberry leaf weight of three age silkworms
For 3.2 ~ 3.6kg, the full mulberry leaf weight of four age silkworms is 11 ~ 13kg.
The step（4）Whole day mulberry leaf amount, weight is 94 ~ 96kg.
The silkworm cultural method for saving mulberry leaf, obtained silkworm.
The silkworm, spin the silk cocoon formed.
Embodiment 2
A kind of silkworm cultural method for saving mulberry leaf, comprises the following steps：
（1）Mulberry leaf harvest：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, nutritious, pH be 4.6 ~
4.8, mulberry leaf can be kept bud green, while metabolite can promote silkworm to grow caused by saccharomycete and lactobacillus-fermented, increase
Strong resistance against diseases, mulberry leaf are saved, reduce silkworm medication, improve silk quality, harvesting amount is to be available for the silkworm amount of 2 ~ 3 days；
（2）One age silkworm：The ambient humidity for keeping silkworm room is 77%, adds new fresh mulberry leaf again after removing residual mulberry leaf every morning, often
The enough mulberry leaf of its feeding, promote growth, improve resistance against diseases；
（3）Two to four age silkworms：Keeping temperature is 26 DEG C, ambient humidity 75%, and it is 14 hours to control light application time, and morning is with
Noon respectively adds mulberry leaf once, and morning, addition was the 38% of whole day mulberry leaf amount, and remaining mulberry leaf are added at noon, improved temperature
Degree, extend illumination, promote the sericterium of silkworm ripe, shorten the growth time of each age silkworm, save mulberry leaf addition, fall asleep first 19 hours
Stop addition mulberry leaf, accelerate to fall asleep；
（4）Fifth instar larva：13 hours first feedings after molting, keeping temperature is 27 DEG C, ambient humidity 74%, and it is 11 to control light application time
Hour, morning, mulberry leaf addition was the 38% of whole day mulberry leaf amount, and morning, mulberry leaf addition was the 33% of whole day mulberry leaf amount, remaining mulberry
Leaf is added at dusk, promotes sericterium growth and ripe, five times in age of shortening, and a small amount of daily repeatedly feeding mulberry leaf, feeding fill
Foot, shorten light application time, silkworm can be made to cocoon concentrations, be easy to be managed collectively, saving man power and material.
The step（1）Fresh-keeping liquid, fountain height be mulberry leaf weight 1.4%, be made up of the raw material of following parts by weight：In vain
Granulated sugar 13, lactic acid bacteria 3, saccharomycete 3, salt 1.3, vitamin C 0.04, white granulated sugar and vitamin C are added to the water, make white sand
The mass concentration of sugar is 7%, accesses lactic acid bacteria, is fermented 11 hours in 39 DEG C, accesses saccharomycete, is fermented 29 hours in 23 DEG C, is added
Salt, stir, obtain fresh-keeping liquid.
Described lactic acid bacteria, it is made up of the bacterial strain of following parts by weight：Lactobacillus acidophilus 14, Lactobacillus plantarum 9, Bulgaria
Lactobacillus 6.
The step（2）New fresh mulberry leaf, each addition be an age silkworm eaten at 8 ~ 10 hours.
The step（3）Whole day mulberry leaf amount, the full mulberry leaf weight of two age silkworms is 1.0 ~ 1.2kg, the full mulberry leaf weight of three age silkworms
For 3.2 ~ 3.6kg, the full mulberry leaf weight of four age silkworms is 11 ~ 13kg.
The step（4）Whole day mulberry leaf amount, weight is 94 ~ 96kg.
The silkworm cultural method for saving mulberry leaf, obtained silkworm.
The silkworm, spin the silk cocoon formed.
Embodiment 3
A kind of silkworm cultural method for saving mulberry leaf, comprises the following steps：
（1）Mulberry leaf harvest：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, nutritious, pH be 4.6 ~
4.8, mulberry leaf can be kept bud green, while metabolite can promote silkworm to grow caused by saccharomycete and lactobacillus-fermented, increase
Strong resistance against diseases, mulberry leaf are saved, reduce silkworm medication, improve silk quality, harvesting amount is to be available for the silkworm amount of 2 ~ 3 days；
（2）One age silkworm：The ambient humidity for keeping silkworm room is 78%, adds new fresh mulberry leaf again after removing residual mulberry leaf every morning, often
The enough mulberry leaf of its feeding, promote growth, improve resistance against diseases；
（3）Two to four age silkworms：Keeping temperature is 26 DEG C, ambient humidity 76%, and it is 15 hours to control light application time, and morning is with
Noon respectively adds mulberry leaf once, and morning, addition was the 40% of whole day mulberry leaf amount, and remaining mulberry leaf are added at noon, improved temperature
Degree, extend illumination, promote the sericterium of silkworm ripe, shorten the growth time of each age silkworm, save mulberry leaf addition, fall asleep first 20 hours
Stop addition mulberry leaf, accelerate to fall asleep；
（4）Fifth instar larva：14 hours first feedings after molting, keeping temperature is 27 DEG C, ambient humidity 75%, and it is 12 to control light application time
Hour, morning, mulberry leaf addition was the 40% of whole day mulberry leaf amount, and morning, mulberry leaf addition was the 35% of whole day mulberry leaf amount, remaining mulberry
Leaf is added at dusk, promotes sericterium growth and ripe, five times in age of shortening, and a small amount of daily repeatedly feeding mulberry leaf, feeding fill
Foot, shorten light application time, silkworm can be made to cocoon concentrations, be easy to be managed collectively, saving man power and material.
The step（1）Fresh-keeping liquid, fountain height be mulberry leaf weight 1.6%, be made up of the raw material of following parts by weight：In vain
Granulated sugar 14, lactic acid bacteria 4, saccharomycete 4, salt 1.4, vitamin C 0.05, white granulated sugar and vitamin C are added to the water, make white sand
The mass concentration of sugar is 8%, accesses lactic acid bacteria, is fermented 12 hours in 40 DEG C, accesses saccharomycete, is fermented 30 hours in 24 DEG C, is added
Salt, stir, obtain fresh-keeping liquid.
Described lactic acid bacteria, it is made up of the bacterial strain of following parts by weight：Lactobacillus acidophilus 15, Lactobacillus plantarum 10, Bao Jiali
Sub- lactobacillus 7.
The step（2）New fresh mulberry leaf, each addition be an age silkworm eaten at 8 ~ 10 hours.
The step（3）Whole day mulberry leaf amount, the full mulberry leaf weight of two age silkworms is 1.0 ~ 1.2kg, the full mulberry leaf weight of three age silkworms
For 3.2 ~ 3.6kg, the full mulberry leaf weight of four age silkworms is 11 ~ 13kg.
The step（4）Whole day mulberry leaf amount, weight is 94 ~ 96kg.
The silkworm cultural method for saving mulberry leaf, obtained silkworm.
The silkworm, spin the silk cocoon formed.
Comparative example 1
Removal step（1）In fresh-keeping liquid, remaining method, with embodiment 1.
Comparative example 2
Step（1）In an age silkworm daily feeding 3 ~ 5 times according to a conventional method, remaining method, with embodiment 1.
Comparative example 3
Step（3）In temperature be 24 DEG C, remaining method, with embodiment 1.
Comparative example 4
Step（3）In be natural lighting, remaining method, with embodiment 1.
Comparative example 5
Step（3）In traditionally carry out feeding, remaining method, with embodiment 1.
Comparative example 6
Step（4）In temperature be 25 DEG C, remaining method, with embodiment 1.
Comparative example 7
Step（4）In be natural lighting, remaining method, with embodiment 1.
Comparative example 8
Step（4）In traditionally carry out feeding, remaining method, with embodiment 1.
Comparative example 9
The tradition of existing silkworm saves the cultural method of mulberry leaf.
The culture efficiency of embodiment and comparative example silkworm cultural method：
The cultivation of silkworm is carried out according to the method for each group respectively, every group of age silkworm one opens, supported respectively according to the method for the group
Grow, observe and record breeding process, record culturing time, required mulberry leaf weight and matured silkworm body weight the results are shown in Table 1, during five ages
The matured silkworm 10 of each group is randomly choosed, according to the method for " measure of Pancreas Glutamate-Pyruvate Transaminase Vigor in several tissues of silkworm, China just etc. ",
The protein content of each group matured silkworm posterior division of silkgland and middle division of silkgland is detected, randomly chooses the silk cocoon each 10 of embodiment and comparative example
Individual, after the silk cocoon of each group is cooked one by one, abundant dryness in the sun to water content is 0 ~ 2%, detects the flat of silk contained by each group silk cocoon
Equal length and average diameter, the results are shown in Table 2.
Table 1：Embodiment and the cultivation situation of comparative example silkworm
| Project | Culturing time/（d） | Mulberry leaf weight/（kg） | Matured silkworm body weight/（G/10 head fresh weights） |
| Embodiment 1 | 24 | 641 | 46.6 |
| Embodiment 2 | 24 | 647 | 46.8 |
| Embodiment 3 | 24 | 644 | 46.9 |
| Comparative example 1 | 25 | 675 | 47.2 |
| Comparative example 2 | 25 | 668 | 47.6 |
| Comparative example 3 | 26 | 684 | 47.4 |
| Comparative example 4 | 26 | 679 | 47.3 |
| Comparative example 5 | 25 | 696 | 47.8 |
| Comparative example 6 | 26 | 715 | 48.1 |
| Comparative example 7 | 26 | 722 | 47.6 |
| Comparative example 8 | 25 | 734 | 48.3 |
| Comparative example 9 | 26 | 788 | 50.3 |
Show from the result of table 1, the silkworm cultural method of the saving mulberry leaf of embodiment, culturing time is substantially short compared with comparative example, makes
Mulberry leaf weight is substantially few compared with comparative example, and matured silkworm body weight is slightly smaller compared with comparative example, illustrates the mulberry of saving mulberry leaf provided by the invention
Silkworm cultural method has the side's of cultivation effect well.
Table 2：The feature of embodiment and comparative example silk
| Project | Posterior division of silkgland/（mg/g） | Middle division of silkgland/（mg/g） | Length/（m） | Diameter/（μm） |
| Embodiment 1 | 17.2 | 4.5 | 1456 | 22.9 |
| Embodiment 2 | 17.2 | 4.1 | 1475 | 23.3 |
| Embodiment 3 | 17.5 | 4.2 | 1512 | 23.1 |
| Comparative example 1 | 13.9 | 3.9 | 1265 | 22.8 |
| Comparative example 2 | 15.2 | 3.8 | 1384 | 23.1 |
| Comparative example 3 | 14.8 | 3.5 | 1253 | 22.6 |
| Comparative example 4 | 13.7 | 3.3 | 1236 | 22.8 |
| Comparative example 5 | 16.2 | 3.2 | 1276 | 23.1 |
| Comparative example 6 | 15.8 | 3.7 | 1295 | 22.4 |
| Comparative example 7 | 13.9 | 3.4 | 1287 | 23.1 |
| Comparative example 8 | 16.8 | 3.2 | 1254 | 22.4 |
| Comparative example 9 | 11.5 | 3.1 | 1114 | 22.5 |
Show from the result of table 2, the silkworm cultural method of the saving mulberry leaf of embodiment, make the posterior division of silkgland and middle division of silkgland of matured silkworm
Protein content it is substantially high compared with comparative example, the silk length contained of single silk cocoon is substantially big compared with comparative example, the diameter of monofilament
With comparative example no significant difference, illustrate that the silkworm cultural method of saving mulberry leaf provided by the invention will not while mulberry leaf are saved
Spinning amount is reduced, spinning amount can be increased on the contrary, there is preferable culture efficiency.
Claims (8)
1. a kind of silkworm cultural method for saving mulberry leaf, it is characterised in that comprise the following steps：
（1）Mulberry leaf harvest：The mulberry leaf harvested every time are transported to mulberry leaf room immediately, uniformly spray fresh-keeping liquid, and harvesting amount is to be available for silkworm 2
The amount of ~ 3 days；
（2）One age silkworm：The ambient humidity for keeping silkworm room is 76 ~ 78%, and fresh mulberry is added again after removing residual mulberry leaf every morning
Leaf；
（3）Two to four age silkworms：Keeping temperature is 26 DEG C, ambient humidity is 74 ~ 76%, and it is 14 ~ 15 hours to control light application time, early
Upper and noon respectively adds mulberry leaf once, and morning, addition was the 35 ~ 40% of whole day mulberry leaf amount, and remaining mulberry leaf are added at noon
Add, stop addition mulberry leaf within 18 ~ 20 hours before falling asleep；
（4）Fifth instar larva：12 ~ 14 hours first feedings after molting, keeping temperature is 27 DEG C, ambient humidity is 73 ~ 75%, when controlling illumination
Between be 11 ~ 12 hours, mulberry leaf addition is the 35 ~ 40% of whole day mulberry leaf amount morning, and morning, mulberry leaf addition is whole day mulberry leaf amount
30 ~ 35%, remaining mulberry leaf are added at dusk.
2. the silkworm cultural method of mulberry leaf is saved according to claim 1, it is characterised in that the step（1）Fresh-keeping liquid,
Fountain height is the 1.2 ~ 1.6% of mulberry leaf weight, is made up of the raw material of following parts by weight：White granulated sugar 12 ~ 14, lactic acid bacteria 2 ~ 4, yeast
Bacterium 2 ~ 4, salt 1.2 ~ 1.4, vitamin C 0.03 ~ 0.05, white granulated sugar and vitamin C are added to the water, and make the quality of white granulated sugar
Concentration is 6 ~ 8%, accesses lactic acid bacteria, is fermented 11 ~ 12 hours in 38 ~ 40 DEG C, accesses saccharomycete, small in 22 ~ 24 DEG C of fermentations 28 ~ 30
When, salt is added, stirs, obtains fresh-keeping liquid.
3. the silkworm cultural method of mulberry leaf is saved according to claim 2, it is characterised in that described lactic acid bacteria, by following
The bacterial strain composition of parts by weight：Lactobacillus acidophilus 13 ~ 15, Lactobacillus plantarum 8 ~ 10, lactobacillus bulgaricus 5 ~ 7.
4. the silkworm cultural method of mulberry leaf is saved according to claim 1, it is characterised in that the step（2）Fresh mulberry
Leaf, each addition are that an age silkworm ate at 8 ~ 10 hours.
5. the silkworm cultural method of mulberry leaf is saved according to claim 1, it is characterised in that the step（3）Whole day mulberry
Ye Liang, the full mulberry leaf weight of two age silkworms are 1.0 ~ 1.2kg, and the full mulberry leaf weight of three age silkworms is 3.2 ~ 3.6kg, the full mulberry leaf weight of four age silkworms
For 11 ~ 13kg.
6. the silkworm cultural method of mulberry leaf is saved according to claim 1, it is characterised in that the step（4）Whole day mulberry
Ye Liang, weight are 94 ~ 96kg.
7. a kind of any one of claim 1 ~ 6 silkworm cultural method for saving mulberry leaf, obtained silkworm.
8. silkworm described in a kind of claim 7, spin the silk cocoon formed.
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| CN114680084A (en) * | 2020-12-29 | 2022-07-01 | 肥西吉新蚕桑专业合作社 | Breeding method for improving activity and health of young silkworms |
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