Summary of the invention
The object of the invention is the physilogical characteristics for laying hen chick stage, immune system is not yet grown completely, Development of Digestive Organs is imperfect, body heat regulation incomcopetence etc., by repeatedly proving and laboratory screening, develop with corn, fermented bean dregs, expanded soybean, soya-bean oil, glucose, compound amino acid, complex enzyme formulation, probiotic etc. as primary raw material, adopt digestible aminoacids design trophic level, and adopt scale of feeding accurately, ensure that chicken searches for food effective nutritional amt, is applicable to novel fodder and the feeding method of big-and-middle-sized laying hen field.
Described feeding method is simple and practical 4-168 nutrient combo application model, this set meal key is the organic assembling of a kind of nutrition science dispensing and accurate scale of feeding technology of each stage stage by stage, efficiently solves the key problem that the current breeding layer chicken level of production is low, of poor benefits.
Described 4-168 set meal pattern-be applicable to brood time, finishing period.
Described 4-168 pattern is specific as follows:
4-brood early stage: 0-2 all high standard chick manufactured feeds, 4 bags/thousand, namely every chicken scale of feeding is 200 grams, and 2 week age, opisthosoma weighed 120 grams;
16-brood the later stage: 3-6 all high standard chick manufactured feeds, 16 bags/thousand, namely every chicken scale of feeding is 800 grams, 6 weekend body weight reach 450 grams;
8-finishing period: 7-15 all high standards are bred as manufactured feeds, and 80 bags/thousand, namely every chicken scale of feeding is 4000 grams, 15 weekend body weight reach 1300 grams;
Described novel fodder nutrient formulation consists of:
1, corresponding set meal 4-nutrient formulation components by weight percent in age in brood time 0-2 week be:
Corn 20-40 part, wheat 10-30 part, flour 1-5 part, expanded soybean 1-6 part, glucose 1-8 part, dregs of beans 10-20 part, corn protein powder 1-3 part, fermented bean dregs 1-5 part, cottonseed protein 1-5 part, fish meal 1-3 part, soya-bean oil 1-3 part, salt 0.2-0.4 part, calcium dihydrogen phosphate 1-1.6 part, stone flour 0.5-1.3 part, synthesizing amino acid 0.2-1.2 part, compound premix 1 part, proteinase-10 .03 part, probiotic 0.05 part, choline 0.05-0.1 part;
2, corresponding set meal 16-the nutrient formulation components by weight percent of brood time 3-6 high standard chick manufactured feed in age in week be: corn 30-50 part, wheat 5-35 part, rice bran 1-8 part, flour 1-8 part, dregs of beans 8-25 part, cottonseed protein 1-5 part, Cottonseed Meal 1-3 part, DDGS1-5 part, salt 0.2-0.4 part, calcium dihydrogen phosphate 1-1.6 part, stone flour 0.7-1.8 part, soya-bean oil 0.5-2 part, synthesizing amino acid 0.1-1.5 part, compound premix 1 part, complex enzyme formulation 0.03-0.08 part, choline 0.05-0.1 part;
3, the formulation weight component of the finishing period high standard incubation manufactured feed of corresponding set meal 8 is: corn 20-55 part, wheat 5-30 part, rice bran 1-8 part, wheat bran 2-12 part, dregs of beans 5-20 part, rapeseed dregs 1-5 part, Cottonseed Meal 1-3 part, DDGS1-8 part, corn bran 1-8 part, salt 0.2-0.4 part, calcium monohydrogen phosphate 1-1.6 part, stone flour 0.5-1.8 part, synthesizing amino acid 0.1-0.8 part, compound premix 1 part, complex enzyme formulation 0.03-0.08 part, probiotic 0.05-0.1 part, choline 0.05-0.1 part.
Described synthesizing amino acid comprises two or more composition of lysine, methionine and threonine.
In described synthesizing amino acid, the parts by weight of lysine, threonine and methionine are: lysine parts by weight are 2-8 part, threonine parts by weight 1-2 part, methionine parts by weight 1-5 part;
Described compound premix is that Liaoning He Feng animal husbandry Co., Ltd produces, and pre-word (2010) 003008 is raised by product authentication code--the Liao Dynasty, and product consists of B B-complex and composite trace element, and trade mark is that standing grain is rich, commercially available.
In described complex enzyme formulation, the parts by weight content of each component is as follows: zytase 1-3 part, mannase 1-3 part, protease 3-6 parts, amylase 5-8 part;
The enzyme activity unit of above-mentioned enzyme is: zytase 20000-30000U/g, mannase 1000-40000U/g, protease 3 000-5000U/g, amylase 3000-6000U/g.
The parts by weight content of each component of described probiotic is as follows: bacillus subtilis microbial agent 2-4 part, Lactobacillus rhamnosus microbial inoculum 1-3 part, saccharomyces cerevisiae microbial inoculum 2-5 part.
Beneficial effect
The present invention according to laying hen physilogical characteristics in vegetative period, the nutrient that dispensing is scientific and reasonable accordingly, and ensure scale of feeding accurately, reach each organ and systematic growth good; And guarantee that laying hen can be up to standard each week age, 15 week age Mo i.e. finishing period terminates body weight and reaches kind requirement namely 1320 grams, importantly body weight and the long uniformity of shin are all more than 90%, cultivation physique is strong, intestinal health state is good, premunition is strong, and replacement pullet is that laying hen voluminous egg in all one's life is laid a solid foundation.Product of the present invention is added with probio bacillus subtilis microbial agent, Lactobacillus rhamnosus microbial inoculum and saccharomyces cerevisiae microbial inoculum in feed.Described microbial inoculum can effectively safeguard laying hen intestinal health, improve its immunity, after particularly feed enters stomach, the glucose that Lactobacillus rhamnosus microbial inoculum utilizes it to adhere in moistening stomach environment ferments, utilize its strong acid producing ability, there is provided environment for exogenous enzyme preparations effectively digests nutriment, promote effective digestion of nutriment; Thus improve the value of final feed.Bacillus subtilis microbial agent,
The interpolation of Lactobacillus rhamnosus microbial inoculum and saccharomyces cerevisiae microbial inoculum effectively can improve the immunity of chicken, improves its resistance against diseases.
Embodiment:
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Lactobacillus provided by the present invention is Lactobacillus rhamnosus (Lactobacillus rhamnosus), this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCCNo.4430, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.Preservation date on December 08th, 2010.
This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is shaft-like, and width is less than 1 μm, and 2 to 3 bacillus are easy to be linked to be and link together; On solid culture medium, this bacterium bacterium colony is milky, and smooth surface is moistening, and thickness, edge is more neat.Compared with original bacteria, this mutagenic strain is morphologically significantly less than starting strain.
Starting strain Lactobacillus rhamnosus CGMCCNo.1.2134 is purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms center.
Lactobacillus rhamnosus of the present invention adopts following flow process to carry out seed selection:
The original bacterial classification that sets out → test tube activation → high temperature acclimation → dithyl sulfate (DES) mutagenesis → high sugared plate screening → nitrosoguanidine (NTG) mutagenesis screening → high temperature bacterium screening → shaking flask sieves → mitotic stability test → 5L fermentation tank test again
Bacterial strain CGMCC No.4430 genetic stability result shows: through continuous passage ten times, property indices is all more stable, and inheritance is better, and proterties is not replied, therefore using the object bacterial strain that bacterial strain CGMCC No.4430 obtains as seed selection.
Object bacterial strain CGMCC No.4430 is done the experiment of 5L lactic acid fermentation tank, result shows: compared with starting strain, and CGMCCNo.4430 glucose-tolerant concentration can reach 270g/L, improves 95% compared with original bacteria; After fermentation ends, lactic acid content is 60g/L, improves 158% compared with original bacteria.
Saccharomyces cerevisiae provided by the present invention (Saccharomyces cerevisiae), this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.4429, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.Preservation date on December 08th, 2010.
This bacterial strain feature is as follows:
Examine under a microscope, the cell of this bacterial strain is avette, one end budding, and size is about 1 × 5 μm; On solid culture medium, this bacterium bacterium colony is milky, and smooth surface is moistening, thickness, and edge is more neat and medium bigger than normal.Compared with original bacteria, this mutagenic strain is morphologically significantly less than starting strain.
Starting strain saccharomyces cerevisiae CICC31481 is purchased from Chinese industrial Microbiological Culture Collection administrative center.
Yeast strain of the present invention adopts following flow process to carry out seed selection:
The original bacterial classification that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → height ooze plate screening → nitrosoguanidine (NTG) mutagenesis screening → height ooze dull and stereotyped primary dcreening operation → shaking flask sieve again → mitotic stability test
The present invention first adopts DES to carry out mutagenesis to starting strain, flat board (150g/L KCl) medium primary dcreening operation is oozed by brewer's wort is high after mutagenesis, then NTG mutagenesis is proceeded to the bacterial strain selected, flat board (200g/L KCl) medium primary dcreening operation is oozed by brewer's wort is high, then adopt 250mL triangular flask to sieve again, the yeast strain that seed selection is excellent, is then cooked passage assays, evaluate its genetic stability, and measure the metabolite content in zymotic fluid with liquid chromatogram, gas chromatograph, gas chromatography mass spectrometry.
Bacterial strain CGMCC No.4429 genetic stability result shows: through continuous passage ten times, property indices is all more stable, and inheritance is better, and proterties is not replied, therefore using the object bacterial strain that bacterial strain CGMCC No.4429 obtains as seed selection.
Object bacterial strain CGMCC No.4429 is done the experiment of 7L fermentation tank, result shows: compared with starting strain, and CGMCC No.4429 initial glucose tolerable concentration can reach 300g/L, improves 50% compared with original bacteria; After fermentation ends, residual sugar is 0.5g/L, glycerol content is 1g/L, improves 10% compared with original bacteria; Acetic acid content is 0.8g/L, lactic acid content is 0.4g/L, and fundamental sum original bacteria is suitable; Concentration of alcohol is 175g/L, improves 108% compared with original bacteria.
The preparation of bacillus subtilis microbial agent:
(1) first order seed is cultivated: bacillus subtilis slant strains accessed in 500 ml shake flasks, medium loading amount 100 milliliters, rotary shaker 180 revs/min, cultivation temperature 30 DEG C, incubation time 24 hours;
(2) secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, medium loading amount 1000 milliliters, rotary shaker 100 revs/min, cultivation temperature 30 DEG C, incubation time 24 hours;
(4) first class seed pot is cultivated: the first class seed pot being 150L with 10% inoculum concentration access total measurement (volume) by three grades of seeds, fermentation medium loading amount 100L, cultivation temperature 28 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours;
(5) fermented and cultured: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification is accessed total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 1 ton, condition of culture cultivation temperature 28 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours, cultivates and terminates bacteria concentration 5.0 × 10
8individual/ml.
(6) centrifugation: adopt centrifuge to be separated and obtain thalline.
(7) vacuum cooling drying: adopt drying process with atomizing process to obtain dry bacteria after adding protectant; Protectant consists of skim milk 10%, lactose 5%, glycerine 5%.Vacuum freeze-drying technique writes culture presevation handbook see Institute of Microorganism, Academia Sinica, within 1980, publishes. 610-653.
Medium forms: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO
31%, pH6.8.
The preparation of Lactobacillus rhamnosus agent:
(1) first order seed is cultivated: accessed by Lactobacillus plantarum bacterial classification in 500 ml shake flasks, medium loading amount 100 milliliters, cultivation temperature 30 DEG C, incubation time 24 hours;
(2) secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, medium loading amount 1000 milliliters, cultivation temperature 30 DEG C, incubation time 24 hours;
(4) first class seed pot is cultivated: the first class seed pot being 150L with 5% inoculum concentration access total measurement (volume) by three grades of seeds, fermentation medium loading amount 100L, cultivation temperature 30 DEG C, tank pressure 0.05Mpa, incubation time 18 hours;
(5) fermentation tank culture: it is 3 tons of secondary seed tanks that first class seed pot bacterial classification is accessed total measurement (volume) with 5% inoculum concentration, fermentation medium loading amount 2 tons, condition of culture cultivation temperature 30 DEG C, tank pressure 0.05Mpa, incubation time 24 hours, cultivates and terminates bacteria concentration 4.0 × 10
8individual/ml.
(6) centrifugation: adopt centrifuge to be separated and obtain thalline.
(7) vacuum cooling drying: add protectant and adopt vacuum refrigeration to carry out dry treatment process, protectant consists of the aqueous solution of skim milk 10%, trehalose 5%, glycerine 5%, glucose 5%.Vacuum freeze-drying technique writes culture presevation handbook see Institute of Microorganism, Academia Sinica, 1980.610-653.2
Medium consists of: casein peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, citric acid diamines 0.2%, Tween800.1%, K
2hPO
40.2%, MgSO
4.7H
2o0.02%, MnSO
4.H
2o0.005%, CaCO
32%, agar 1.5%, pH6.8.
Bacillus subtilis CGMCC1.210, saccharomyces cerevisiae CGMCC4429, Lactobacillus rhamnosus CGMCC4430.
Embodiment 1
The parts by weight of the nutrient formulation component of 0-2 high standard chick manufactured feed in age in week are:
Corn 36.3 parts, wheat 20.01 parts, 4.0 parts, flour, expanded soybean 5 parts, glucose 3 parts, dregs of beans 17.2 parts, corn protein powder 1.1 parts, fermented bean dregs 3 parts, cottonseed protein 2 parts, fish meal 1 part, 2 parts, soya-bean oil, salt 0.3 part, calcium dihydrogen phosphate 1.3 parts, stone flour 1.2 parts, synthesizing amino acid 1 part, compound premix 1 part, proteinase-10 .03 part, probiotic 0.05 part, 0.1 part, choline.
3-6 age in week high standard chick manufactured feed the parts by weight of nutrition composition be:
Corn 42.5 parts, wheat 15.5 parts, 4.3 parts, rice bran, 5 parts, flour, dregs of beans 18 parts, cottonseed protein 3 parts, Cottonseed Meal 1.5 parts, DDGS4.6 part, salt 0.3 part, calcium dihydrogen phosphate 1.5 parts, stone flour 1.38 parts, 0.8 part, soya-bean oil, synthesizing amino acid 1 part, compound premix 1 part, complex enzyme formulation 0.05 part, 0.08 part, choline.
7-15 age in week high standard laying hen finishing period manufactured feed the parts by weight of nutrition composition be:
Corn 47.76 parts, wheat 5.5 parts, 4.1 parts, rice bran, wheat bran 12 parts, dregs of beans 10.0 parts, rapeseed dregs 3 parts, Cottonseed Meal 1 part, DDGS7.1 part, corn bran 5 parts, salt 0.35 part, calcium monohydrogen phosphate 1.2 parts, stone flour 1.3 parts, synthesizing amino acid 0.5 part, complex enzyme formulation 0.04 part, probiotic 0.05 part, 0.05 part, choline.
In described synthesizing amino acid, the parts by weight of lysine, threonine and methionine are: lysine parts by weight are 5 parts, threonine parts by weight 1 part, methionine parts by weight 3 parts.
In described complex enzyme formulation, the parts by weight content of each component is as follows: zytase 2 parts, mannase 2 parts, 5 parts, protease, amylase 6 parts.
The parts by weight content of each component of described probiotic is as follows: bacillus subtilis microbial agent 3 parts, Lactobacillus rhamnosus microbial inoculum 2 parts, saccharomyces cerevisiae microbial inoculum 3 parts.
Embodiment 2
The nutrient formulation mass percent of 0-2 high standard chick manufactured feed in age in week consists of:
Corn 20 parts, wheat 10 parts, 1 part, flour, expanded soybean 1 part, glucose 1 part, dregs of beans 10 parts, corn protein powder 2 parts, fermented bean dregs 1 part, cottonseed protein 1 part, fish meal 1 part, 1 part, soya-bean oil, salt 0.2 part, calcium dihydrogen phosphate 1 part, stone flour 0.5 part, synthesizing amino acid 0.2 part, compound premix 1 part, proteinase-10 .03 part, probiotic 0.05 part, acidulant 0.3 part, 0.05 part, choline.
The nutrition technique embodiment of 3-6 high standard chick manufactured feed in age in week is:
Corn 30 parts, wheat 5 parts, 1 part, rice bran, 1 part, flour, dregs of beans 8 parts, cottonseed protein 1 part, Cottonseed Meal 1 part, DDGS1 part, salt 0.2 part, calcium dihydrogen phosphate 1 part, stone flour 0.7 part, 0.5 part, soya-bean oil, synthesizing amino acid 0.1 part, compound premix 1 part, complex enzyme formulation 0.03 part, 0.05 part, choline.
7-15 week age high standard laying hen finishing period manufactured feed nutrition technique embodiment be:
Corn 20 parts, wheat 20 parts, 1 part, rice bran, wheat bran 2 parts, dregs of beans 5 parts, rapeseed dregs 1 part, Cottonseed Meal 2 parts, DDGS1 part, corn bran 1 part, salt 0.2 part, calcium monohydrogen phosphate 1 part, stone flour 0.5 part, synthesizing amino acid 0.1 part, complex enzyme formulation 0.03 part, probiotic 0.08 part, 0.08 part, choline.
In described synthesizing amino acid, the parts by weight of lysine, threonine and methionine are: lysine parts by weight are 2 parts, threonine parts by weight 1 part, methionine parts by weight 1 part.
In described complex enzyme formulation, the parts by weight content of each component is as follows: zytase 1 part, mannase 1 part, protease 3 part, amylase 5 parts.
The parts by weight content of each component of described probiotic is as follows: bacillus subtilis microbial agent 2 parts, Lactobacillus rhamnosus microbial inoculum 1 part, saccharomyces cerevisiae microbial inoculum 2 parts.
Embodiment 3
The nutrient formulation mass percent of 0-2 high standard chick manufactured feed in age in week consists of:
Corn 40 parts, wheat 30 parts, 5 parts, flour, expanded soybean 6 parts, glucose 8 parts, dregs of beans 20 parts, corn protein powder 3 parts, fermented bean dregs 5 parts, cottonseed protein 5 parts, fish meal 3 parts, 3 parts, soya-bean oil, salt 0.4 part, calcium dihydrogen phosphate 1.6 parts, stone flour 1.3 parts, synthesizing amino acid 1.2 parts, compound premix 1 part, proteinase-10 .03 part, probiotic 0.05 part, 0.1 part, choline.
3-6 week age high standard chick manufactured feed nutrition technique embodiment be:
Corn 50 parts, wheat 35 parts, 8 parts, rice bran, 8 parts, flour, dregs of beans 25 parts, cottonseed protein 5 parts, Cottonseed Meal 3 parts, DDGS5 part, salt 0.4 part, calcium dihydrogen phosphate 1.6 parts, stone flour 1.8 parts, 2 parts, soya-bean oil, synthesizing amino acid 1.5 parts, compound premix 1 part, complex enzyme formulation 0.08 part, 0.1 part, choline.
The nutrition technique embodiment of 7-15 high standard laying hen finishing period manufactured feed in age in week is
Corn 55 parts, wheat 30 parts, 8 parts, rice bran, wheat bran 12 parts, dregs of beans 20 parts, rapeseed dregs 5 parts, Cottonseed Meal 3 parts, DDGS8 part, corn bran 8 parts, salt 0.4 part, calcium monohydrogen phosphate 1.6 parts, stone flour 1.8 parts, synthesizing amino acid 0.8 part, complex enzyme formulation 0.08 part, probiotic 0.1 part, 0.1 part, choline.
In described synthesizing amino acid, the parts by weight of lysine, threonine and methionine are: lysine parts by weight are 8 parts, threonine parts by weight 1.5 parts, methionine parts by weight 5 parts.
In described complex enzyme formulation, the parts by weight content of each component is as follows: zytase 1 part, mannase 1 part, protease 3 part, amylase 5 parts.
The parts by weight content of each component of described probiotic is as follows: bacillus subtilis microbial agent 4 parts, Lactobacillus rhamnosus microbial inoculum 3 parts, saccharomyces cerevisiae microbial inoculum 5 parts.
Result of use experimental example
Employing embodiment inspires the 4-168 laying hen nutrition set meal application model technical combinations of educating strong physique and does following test with the scheme combined of precisely feeding.
1 test material
1.1 test period
Tested on December 25th, 1 2011 on September 10th, 2011, last 105 days.
1.2 test site
Test and implement at the rich Experimental Base of Huan Ren Xinhua of Liaoning Province chicken house standing grain.
Control group is common laying hen material, 0-6 ages in week, 7-15 ages in week.Experimental group is that the present invention one group that feeds inspires the 4-168 laying hen nutrition set meal application model of educating strong physique, and divided stages was 0-2 ages in week, 3-6 ages in week, 7-12 ages in week.
Test chicken divides into groups: test adopts single factor experiment design, and experiment material selects 3600 blue brown 1 age in days laying hens in sea, and be divided into 2 groups, often group establishes 6 repetitions, each repetition 300 chickens;
Fed control group and feed group of the present invention respectively.
Feeding method: control group is according to existing market situation free choice feeding; Experimental group precisely measure according to 4-168 set meal pattern fed for 0-2 ages 200 grams in week/only, 3-6 ages 800 grams in week/only, 7-15 ages 4000 grams in week/only.
Feeding and management is carried out according to chicken house daily management.This experiment proceeds to 105 days continuously.
Testing index: 2 weekend body weight, 6 weekend body weight, 15 weekend body weight; Tibia length (2 weekends, 6 weekends, 15 weekends), calculates the body weight uniformity and the shin bone uniformity; Dead wash in a pan number, calculate brood be bred as at the end of survival rate; Feed intake.
Table 1 the present invention is on the impact of Laying chicks 2 productivity at weekend
Note: female different expression significant difference (P < 0.05) of colleague's shoulder marking-up.
Table 2 the present invention is on the impact of laying hen 6 productivity at weekend
Note: female different expression significant difference (P < 0.05) of colleague's shoulder marking-up.
Table 3 the present invention is on the impact of laying hen 15 productivity at weekend
Note: female different expression significant difference (P < 0.05) of colleague's shoulder marking-up.
As can be seen from above result of the test, feed group of the present invention is up to standard in body weight, Tibia Development, and the many indexs such as bodily form body condition and survival rate measure and are all obviously better than common material group, have absolutely proved science and the advance of product of the present invention.Antibiotic dosage test shows: experimental group reduces by 75.3% than control group antibiotic dosage.Adopt ten point system standard to give a mark to the hair color of chicken, marking value is 9.6 points, and control group is 7.1 points simultaneously.Result shows that product of the present invention can improve body immunity, reduces antibiotic dosage, increases cultivation quality and benefits.