CN102648704A - Method for making mulberry silkworm spin glossy dark green silkworm cocoon - Google Patents
Method for making mulberry silkworm spin glossy dark green silkworm cocoon Download PDFInfo
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- CN102648704A CN102648704A CN2012101518057A CN201210151805A CN102648704A CN 102648704 A CN102648704 A CN 102648704A CN 2012101518057 A CN2012101518057 A CN 2012101518057A CN 201210151805 A CN201210151805 A CN 201210151805A CN 102648704 A CN102648704 A CN 102648704A
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- silkworm
- dark green
- mulberry
- glossy dark
- silk
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Abstract
The invention relates to a mulberry silkworm breeding technology, and aims at providing a method for making mulberry silkworm spin glossy dark green silkworm cocoon. The method comprises the following steps of: selecting and hatching eggs of mulberry silkworm hybrid varieties-HUABI and LVZHU, breeding hybrid silkworm larvae to the fourth day in the fifth instar stage for standby according to a standardized mulberry silkworm breeding method; preparing Nile blue hydrochloride aqueous solution with percentage concentration by weight of 0.01 percent; spraying the Nile blue hydrochloride aqueous solution uniformly onto mulberry leaves by utilizing an atomizer, spraying 1L of feed additives onto 7kg of fresh mulberry leaves, and then feeding the mulberry silkworm at the fourth day in the fifth instar stage with the fresh mulberry leaves after being slightly dried; continuing the feeding method until the mounting of the silkworm to acquire the glossy dark green mulberry silkworm. The mulberry leaves treated with the additives have no toxicity or side effect on the mulberry silkworm, pigment can penetrate, can be assimilated by the intestinal wall of fifth instar silkworm larvae and can enter silkgland cells through a silkgland body so as to secrete the glossy dark green silk protein, so that the silkworm can spin glossy dark green silk and spin the glossy dark green cocoon, and the silk has an ideal color fastness. The feed additives and the application technology are free from utilizing any antibiotic and assistant, and the method is simple and environment-friendly.
Description
Technical field
The present invention relates to the silkworm breeding technology, particularly a kind of method that makes silkworm tie glossy dark green look silk cocoon.
Technical background
Along with expanding economy, the raising of people's living standard is to the requirement of dress ornament also more and more personalization, comfortableization, high materialization and environmental protectionization.Therefore, silk fabrics still can become the product that is full of glamour and hope.The silk production of China at present occupies absolute leading position in the world.But because kind product single and low surcharge is main, lack the competitive advantage on the price in the international market for a long time.How to adopt new and high technology, exploitation characteristic silk new product has become the key of promoting traditional silk industry.Traditional silk stresses that already the raw material cocoon must be pure white, filature or knit silk fabric after dye again, obtain multicoloured silk fabrics.But; The modern advocates nature, to the reinforcement of environmental consciousness, produces natural colorful silk; Healthy and helpful to people not only; And can avoid printing and dyeing industry to the pressure that environmental protection caused, and be a kind of environmental friendly product that extensive prospect is arranged, meet the industry sustainable development policy of country.The method of producing colorful silk at present has following several kinds:
1. utilize the natural colored cocoon gene of silkworm breeding natural color cocoon silkworm kind; Though the silkworm kind of this method seed selection can be produced redness, golden yellow and yellow green silk cocoon; But the raw silk of reel silk from cocoons system all is a Yellow series, because pigment is many in silk gum, the raw silk refining process fades seriously; Its product is easy to decolouring after seeing light, so be difficult to practicability.
2. utilize bionic transgenosis means to produce natural colorful silk, this technology is consuming time expensive big, and technical difficulty is high, still can only produce the green fluorescence cocoon at present, and fluorescent lamp shows greenly down in the dark, so Practical significance is little.
3. in feed, add pigment,, up to the present can only produce redness, yellow and blue silk cocoon, can not produce the silk cocoon of green-series with the method because silkworm is very responsive to chemical substance.
Summary of the invention
The technical problem that the present invention will solve is, overcomes deficiency of the prior art, provides a kind of and can make silkworm secrete glossy dark green look fibroin, and the silk cocoon of tying is the uniform glossy dark green look of color, and the silk of preparation is not met with the breeding technology that light fades.
For solving the problems of the technologies described above, the invention provides a kind of method that makes silkworm tie glossy dark green look silk cocoon, may further comprise the steps:
(1) select for use the silkworm crossbreed green * hatching of hastening the hatching of silkworms of the silkworm seed of green pearl, it is subsequent use the 4th day age to raise its crossbreed silkworm larva to five by standardization silkworm raising method;
(2) preparation can make silkworm secrete the feed addictive of glossy dark green look fibroin, and this feed addictive is that weight percent concentration is 0.01% the Nile blue hydrochloride aqueous solution;
(3) said feed addictive evenly is sprayed on the mulberry leaf the new fresh mulberry leaf of 1L additive spray 7kg, the silkworm of feeding the 4th day five ages after dried slightly with sprayer; Continue this feeding method until being placed on small straw bundles to spin cocoons, obtain the silk cocoon of glossy dark green look.
The Nile blue hydrochloride is claimed Nile blue again, Nile blue.English name Nile Blue A (CAS:3625-57-8), molecular weight: 732.85, molecular formula: C
40H
40N
6O
2.O
4S; Green crystal property powder has metallic luster.Be dissolved in hot water and be blue, be slightly soluble in cold water and ethanol.As acid-base indicator, color change interval pH value 10.1 (indigo plant)～11.1 (red) is usually used in preparing the soda acid mixed indicator; Also as biological stain.
The present invention has following obvious advantage:
The mulberry leaf of this additive treating are nontoxic without any side effects to silkworm; Pigment can permeate by five age silkworm the intestines wall absorb and see through the sericterium body and get into silk gland cell; Secrete glossy dark green look fibroin; Thereby make the spue silk of glossy dark green look of silkworm, bear the silk cocoon of glossy dark green look, and silk has very good color fastness.Feed addictive of the present invention and operation technique need not used any antibiotic and assistant agent, and method is simple, environmental protection.
Embodiment
The crossbreed silkworm egg of spending green and green pearl that uses among the present invention, its direct sources is: available from animal science institute of Zhejiang University, any public all can get in touch and obtain voluntarily.Spending green and green pearl is the variety source that professor Chen Yuyin of animal science institute of Zhejiang University utilizes this laboratory long preservation; Adopt the marker assisted selection means; Use the special-purpose new varieties that crossbreeding technology is cultivated through directive breeding for many years, Zhejiang University has intellectual property.
The original seed primary source of crossbreed silkworm is respectively: Hangzhoupro flower, Bi Lian, P50, Soviet Union 12, and these genetic resources kinds all come from animal science institute of Hangzhou, Zhejiang province city Zhejiang University, and all kind primary sources all are Chinese Zhejiang Province.
Strong good the supporting of its larva physique of this crossbreed silkworm; Its larva contains the Gc gene; The Gc gene is given silkworm body midgut epithelial cells and is had the ability that absorbs said additive pigment; And can see through silk gland cell from blood with action of chemicals artificial oil green pigment in the silkworm body, make the sericterium body secrete glossy dark green look silkworm and mulberry albumen.
In the present embodiment, may further comprise the steps:
A kind of method that makes silkworm tie glossy dark green look silk cocoon that present embodiment provides comprises the steps:
(1) select for use the silkworm crossbreed green * silkworm seed of green pearl is according to the routine techniques hatching of hastening the hatching of silkworms, it is subsequent use the 4th day age to raise its crossbreed silkworm larva to five by standardization silkworm raising method;
(2) preparing the feed addictive that can make silkworm secrete glossy dark green look fibroin is the Nile blue hydrochloride aqueous solution, English name Nile Blue A (CAS:3625-57-8), and molecular weight: 732.85, molecular formula: C
40H
40N
6O
2.O
4S; By weight, contain Luo Lan hydrochloride 0.01% in the said aqueous solution.
(3) will the time state the feed addictive aqueous solution and evenly be sprayed on the mulberry leaf with sprayer, the new fresh mulberry leaf of 1L additive spray 7kg, the silkworm of feeding the 4th day five ages after dried slightly, add food after one day the silkworm larva body colour become blue.Continue to cocoon to being placed on small straw bundles to spin cocoons, can obtain the silk cocoon of glossy dark green look with said method raising.
All distortion of from content disclosed by the invention, deriving or associating it is to be noted that above enumerating only is some specific embodiments of the present invention, the invention is not restricted to above instance, also many distortion can be arranged, if all should be thought protection scope of the present invention.
Claims (1)
1. a method that makes silkworm tie glossy dark green look silk cocoon is characterized in that, may further comprise the steps:
(1) select for use the silkworm crossbreed green * hatching of hastening the hatching of silkworms of the silkworm seed of green pearl, it is subsequent use the 4th day age to raise its crossbreed silkworm larva to five by standardization silkworm raising method;
(2) preparation can make silkworm secrete the feed addictive of glossy dark green look fibroin, and this feed addictive is that weight percent concentration is 0.01% the Nile blue hydrochloride aqueous solution;
(3) said feed addictive evenly is sprayed on the mulberry leaf the new fresh mulberry leaf of 1L feed addictive spray 7kg, the silkworm of feeding the 4th day five ages after dried slightly with sprayer; Continue this feeding method until being placed on small straw bundles to spin cocoons, obtain the silk cocoon of glossy dark green look.
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| CN2012101518057A CN102648704A (en) | 2012-05-16 | 2012-05-16 | Method for making mulberry silkworm spin glossy dark green silkworm cocoon |
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| CN2012101518057A CN102648704A (en) | 2012-05-16 | 2012-05-16 | Method for making mulberry silkworm spin glossy dark green silkworm cocoon |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986602A (en) * | 2012-12-28 | 2013-03-27 | 苏州大学 | Method for cultivating natural green cocoon silkworm variety |
| CN103004699A (en) * | 2012-12-19 | 2013-04-03 | 安徽省农业科学院水产研究所 | Snail ecological breeding system |
| CN103416354A (en) * | 2013-07-31 | 2013-12-04 | 李学义 | Mulberry shoot silkworm breeding method achieved through topping, repeated shearing and leaf picking |
| CN104705261A (en) * | 2015-03-12 | 2015-06-17 | 霍山县天衡桑蚕制种有限公司 | Cultivating method of colorized cocoon |
| CN105903027A (en) * | 2016-05-31 | 2016-08-31 | 福建傲农生物科技集团股份有限公司 | Method for long-term preservation of antibiotics at room temperature |
| US10160058B2 (en) | 2012-08-17 | 2018-12-25 | Nippon Steel & Sumitomo Metal Corporation | Electric resistance welded pipe welding apparatus |
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| JP2000000094A (en) * | 1998-06-15 | 2000-01-07 | Natl Inst Of Sericultural & Entomological Science | SILKWORM Bm white GENE |
| JP2005143428A (en) * | 2003-11-18 | 2005-06-09 | National Institute Of Agrobiological Sciences | Utilization of dna encoding kynurenine oxidase gene of silkworm |
| CN101285215A (en) * | 2008-06-12 | 2008-10-15 | 江苏苏豪国际集团股份有限公司 | Process for preparing yellow silk |
| CN101411397A (en) * | 2008-11-11 | 2009-04-22 | 苏州大学 | Feedstuff additive for preparing coloured cocoon fibre |
-
2012
- 2012-05-16 CN CN2012101518057A patent/CN102648704A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000000094A (en) * | 1998-06-15 | 2000-01-07 | Natl Inst Of Sericultural & Entomological Science | SILKWORM Bm white GENE |
| JP2005143428A (en) * | 2003-11-18 | 2005-06-09 | National Institute Of Agrobiological Sciences | Utilization of dna encoding kynurenine oxidase gene of silkworm |
| CN101285215A (en) * | 2008-06-12 | 2008-10-15 | 江苏苏豪国际集团股份有限公司 | Process for preparing yellow silk |
| CN101411397A (en) * | 2008-11-11 | 2009-04-22 | 苏州大学 | Feedstuff additive for preparing coloured cocoon fibre |
Non-Patent Citations (2)
| Title |
|---|
| JOHN M.KOLYER: "The feeding of coloring matters to Pieris Rapae larvae", 《JOURNAL OF RESEARCH OF THE LEPIDOPTERA》, vol. 4, no. 3, 31 December 1965 (1965-12-31), pages 159 - 172 * |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10160058B2 (en) | 2012-08-17 | 2018-12-25 | Nippon Steel & Sumitomo Metal Corporation | Electric resistance welded pipe welding apparatus |
| CN103004699A (en) * | 2012-12-19 | 2013-04-03 | 安徽省农业科学院水产研究所 | Snail ecological breeding system |
| CN103004699B (en) * | 2012-12-19 | 2014-10-22 | 安徽省农业科学院水产研究所 | Snail ecological breeding system |
| CN102986602A (en) * | 2012-12-28 | 2013-03-27 | 苏州大学 | Method for cultivating natural green cocoon silkworm variety |
| CN103416354A (en) * | 2013-07-31 | 2013-12-04 | 李学义 | Mulberry shoot silkworm breeding method achieved through topping, repeated shearing and leaf picking |
| CN104705261A (en) * | 2015-03-12 | 2015-06-17 | 霍山县天衡桑蚕制种有限公司 | Cultivating method of colorized cocoon |
| CN105903027A (en) * | 2016-05-31 | 2016-08-31 | 福建傲农生物科技集团股份有限公司 | Method for long-term preservation of antibiotics at room temperature |
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Application publication date: 20120829 |