CN104430214B - A kind of robustness is from breeding method and the application going out the double sex-limited cultivated silkworm breed variety of cocoon speckle - Google Patents

Abstract

The invention belongs to silkworm improved seeds and cultivate field, be specifically related to a kind of robustness from breeding method and the application going out the double sex-limited cultivated silkworm breed variety of cocoon speckle.Method particularly includes: first speckle sex limited gene is imported in production Silkworm race, thus improve the integral economic trait of speckle autosexing race, hybridization is used to import short period of duration blood relationship again, certainly go out the methods such as cocoon in conjunction with Bombycis mori speckle autosexing race is hybridized and domestication and improvement, this kind cultivated can utilize the sex-limited feature of speckle of silkworm by female male silkworm separately in larval phase, and can sprout wings by cocoon-break voluntarily in pupa time, certainly go out cocoon rate and reach more than 95%.The double autosexing race of speckle that the present invention cultivates has that period of duration is short, body constitution is strong, Quality of Stored Cocoon And Silk is excellent, certainly go out cocoon rate high.

Description

A kind of robustness is from breeding method and the application going out the double sex-limited cultivated silkworm breed variety of cocoon speckle

Technical field

The invention belongs to silkworm improved seeds and cultivate field, be specifically related to a kind of robustness from going out the double limit of cocoon speckle The breeding method of property cultivated silkworm breed variety and application.

Background technology

Silkworm egg is the important means of production that Sericultural production is indispensable, is that Silk industry is depended on for existence and development Basis.But owing to being continuously increased by labor cost, the factor such as the means of production significantly rise in price affected, cause silkworm Plant field benefit to go from bad to worse, add that China's economic society fast development, urbanization paces are accelerated, rural surplus Labour force is in short supply, ageing phenomenon is serious, and particularly seed station cuts the cocoon point male and female link working time at kind of a cocoon Tightly, task weight, recruitment cost rises steadily (such as seed station day work just 10 yuan/person/day when 1996, but closely Year has risen to 110～120 yuan/person/day) and the most also ask not enough people, these problems the most seriously govern silkworm egg The survival and development of production unit, cause indivedual seed station the most significantly to lose, production scale and productivity effect Seriously it is wound.

The sex-limited system of speckle is on speckle group translocation to W chromosome, makes the female for speckle of incubation Silkworm varieties Silkworm, hero is element speckle silkworm.The double sex-limited Silkworm varieties of speckle, it is simply that in, Japanese original seed and common species, in larval phase Can carry out labelling sex with obvious speckle, the most female silkworm is common speckle (flower silkworm), and male silkworm is element speckle (white silkworm), Thus the special silkworm of male and female sex by the presence or absence of speckle, can be distinguished exactly in 4～5 ages of larva Kind.But under normal circumstances, the conventional Silkworm varieties of the most existing production or the sex-limited Silkworm varieties of speckle, Owing to its Cocoon layer ratio is generally higher than 20%, cause Bombycis mori sprout wings after more difficult cocoon-break voluntarily and go out, therefore produce on Go out cocoon rate to improve the emergence of Bombycis mori, commonly used cut cocoon after sprout wings again, copulation and the production of hybrid seeds, these are the most Cut cocoon and male and female in pupa time judge the low produced production cost of efficiency.Therefore Labor-saving high-efficiency new silkworm breed is accelerated Cultivate, it is achieved with the sex-limited feature of speckle utilizing the silkworm larva phase, female male silkworm is separated and the Bombycis mori cocoon-break production of hybrid seeds voluntarily Labor-saving, high efficiency breeding objective, extremely the most urgent, to help seed station solve and be released in kind Cocoon cuts the labour demand of cocoon point male and female link, reduces a large amount of recruitments of production of hybrid seeds link, significantly improves seed station Labor efficiency and economic benefit have important practical significance.Meanwhile, going deep into along with Comprehensive Unilization of Silkworm Mulberry Expanding, the application prospect of the non-raw silk Silkworm varieties with larva speckle labelling sex, Bombycis mori cocoon-break voluntarily is wide Wealthy.

Summary of the invention

In order to overcome the shortcoming of prior art with not enough, the primary and foremost purpose of the present invention is to provide a kind of robustness From the breeding method going out the double sex-limited cultivated silkworm breed variety of cocoon speckle.

Another object of the present invention is to provide the application of above-mentioned breeding method.

The purpose of the present invention is achieved through the following technical solutions:

A kind of robustness goes out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle certainly, comprises the steps of:

(1) respectively with middle system, Japanese pattern of silkworm autosexing race as female parent, with middle system, Japanese existing production Being male parent with cultivated silkworm breed variety, hybridization obtains new man of middle system pattern of silkworm autosexing race and new Japanese silkworm respectively Speckle autosexing race；New man of middle system pattern of silkworm autosexing race and Japanese new pattern of silkworm to obtaining are sex-limited In F1～the F5 generation of kind, is respectively adopted 0.2～1.0g ant mixing and educates, and F6 generation begins with one batch rearing, selects tool Reserve seed for planting selection-breeding in individuality that finiteness speckle and cocoon shape, the nearly male parent of cocoon color, integral economic trait are good or moth district, logical Too much for directive breeding, cultivate and possess the sex-limited feature of speckle, and integral economic trait reaches or close production With the new speckle autosexing race of kind, thus realize importing in production Silkworm varieties by speckle sex limited gene, Improve the purpose of the integral economic trait of speckle autosexing race；

(2) respectively with new man of the middle system pattern of silkworm autosexing race cultivated in step (1), new Japanese Family pattern of silkworm autosexing race be female parent, with in be short period of duration low Cocoon layer ratio kind, the Japanese low cocoon of short period of duration Layer rate kind is male parent, and hybridization is short period of duration speckle autosexing race and Japanese short period of duration speckle limit in obtaining Property kind；To obtain in be short period of duration speckle autosexing race and Japanese short period of duration speckle autosexing race In F1～F4 generation, is respectively adopted 0.2～1.0g ant mixing and educates, and selects the individuality that integral economic trait is good, uses silkworm Moth is from the method moth appearance production of hybrid seeds going out cocoon；In F5 generation, begins with one batch rearing, and select integral economic trait good Ti Huoe district, uses Bombycis mori to reserve seed for planting selection-breeding from the method going out cocoon；Selected above through 7 generations, cultivate respectively From go out cocoon rate and reach in more than 95% be the short sex-limited Silkworm varieties of period of duration speckle and Japanese short period of duration speckle sex-limited Silkworm varieties；

(3) new man of the middle system pattern of silkworm using Bombycis mori to cultivate step (1) from the method going out cocoon is sex-limited Kind, Japanese pattern of silkworm autosexing race proceed by domestication and improvement from F16 so that it is certainly go out cocoon rate and improve extremely More than 95%, it is thus achieved that man of the middle system pattern of silkworm autosexing race after domestication and improvement, Japanese pattern of silkworm autosexing race；

(4) respectively during step (2) is cultivated to be the short sex-limited Silkworm varieties of period of duration speckle, Japanese short Man of middle system pattern of silkworm autosexing race after domestication and improvement, day in the sex-limited Silkworm varieties of period of duration speckle and step (3) Be family's pattern of silkworm autosexing race be parent, with in × in, day × day be made into double cross original seed, in, Japanese double cross Original seed hybridization obtains quaternary first cross combination, is robustness from going out the double sex-limited cultivated silkworm breed variety of cocoon speckle；

Man of the middle system pattern of silkworm autosexing race as female parent described in step (1) is family's pattern of silkworm in being preferably Autosexing race " SLC-13 "；

Japanese the pattern of silkworm autosexing race as female parent described in step (1) is preferably Japanese pattern of silkworm Autosexing race " SLJ-01 "；

Described in step (1) as male parent is existing production cultivated silkworm breed variety be preferably in be existing life Produce with cultivated silkworm breed variety " 932 "；

The Japanese existing production cultivated silkworm breed variety as male parent described in step (1) is preferably Japanese existing life Produce with cultivated silkworm breed variety " 7532 "；

Described in step (2) as male parent is short period of duration low Cocoon layer ratio kind be preferably in be short life Phase of educating low Cocoon layer ratio kind " SGC-01 "；

The Japanese short period of duration low Cocoon layer ratio kind as male parent described in step (2) is preferably Japanese short life Phase of educating low Cocoon layer ratio kind " SGJ-01 "；

Described Bombycis mori is after the Pupa bombycis moth that the method going out cocoon refers in Bombyx bombycis, and Bombycis mori is by secretion one Bombyx bombycis is broken a duck eye by protease molten cocoon enzyme, and then Bombycis mori crawls out and carries out mate and oviposit；

Integral economic trait described in step (1), (2) is preferably well worm pupa rate 60%～100%, whole cocoon Amount 1.00g～2.00g, Cocoon layer ratio 13.00%～28.00%；

Domestication and improvement described in step (3) refers to raise the kind of cultivation under the conditions of high temperature and humidity, then Select the individual method moth appearance production of hybrid seeds using Bombycis mori certainly to go out cocoon that integral economic trait is good, select certainly go out cocoon Individuality is reserved seed for planting subculture；

Described high temperature and humidity condition is preferably temperature 25 DEG C～32 DEG C, humidity 65%～100%；

Step (3) makes the vitality of cultivation kind reach or close to real preferably through repeatedly domestication and improvement with realization By the level of kind with certainly go out cocoon rate and reach the target of more than 95%；

Described robustness, from going out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle, preferably comprises following steps:

(1) respectively with man of middle system pattern of silkworm autosexing race " SLC-13 ", Japanese pattern of silkworm autosexing race " SLJ-01 " is maternal, with in be that existing production cultivated silkworm breed variety " 932 ", Japanese existing production use silkworm product Planting " 7532 " is male parent, is speckle autosexing race " SLC-14 " and Japanese speckle during hybridization acquisition is new respectively Stricture of vagina autosexing race " SLJ-02 ", is respectively adopted 0.5g to F1～the F5 generation of SLC-14 and SLJ-02 obtained Ant mixing is educated, and F6 generation begins with one batch rearing, selects have sex-limited speckle and cocoon shape, cocoon color close relative basis 932 With 7532, the good individuality of integral economic trait or moth district reserve seed for planting subculture；By many for directive breeding, cultivate Possess the sex-limited feature of speckle, and integral economic trait reaches or close to the new sex-limited product of speckle of producer goods kind Plant SLC-14 and SLJ-02, thus realize importing in production Silkworm varieties by speckle sex limited gene, improve speckle The purpose of the integral economic trait of autosexing race；

(2) man of middle system pattern of silkworm autosexing race " SLC-14 " that cultivates with step (1) respectively, Japanese Family pattern of silkworm autosexing race " SLJ-02 " be female parent, with in be short period of duration low Cocoon layer ratio kind " SGC-01 ", In Japanese short period of duration low Cocoon layer ratio kind " SGJ-01 ", the individuality of larva element speckle is male parent, during hybridization obtains It is short period of duration speckle autosexing race " SGSLC " and Japanese short period of duration speckle autosexing race " SGSLJ "； F1～F4 of " SGSLC " and " SGSLJ " obtained is educated for being respectively adopted the mixing of 0.5g ant, selects The individuality that integral economic trait is good, uses Bombycis mori from the method moth appearance production of hybrid seeds going out cocoon；In F5 generation, begins with unilateral tonsillitis Educate, and select individuality or the moth district that integral economic trait is good, use Bombycis mori to reserve seed for planting subculture from going out cocoon method；Warp Selected above after 7 generations, cultivation come from cocoon rate to reach in more than 95% respectively is the short sex-limited silkworm of period of duration speckle Kind " SGSLC " and the sex-limited Silkworm varieties of Japanese short period of duration speckle " SGSLJ "；

(3) use Bombycis mori from go out " SLC-14 " and " SLJ-02 " that step (1) cultivated by cocoon method from F16 proceeds by domestication and improvement, to improve it from going out cocoon rate so that it is certainly go out cocoon rate and improve to more than 95%, obtain Obtain man of the middle system pattern of silkworm autosexing race after domestication and improvement, Japanese pattern of silkworm autosexing race, be respectively designated as " SLC-14 is certainly " and " SLJ-02 is certainly "；

(4) respectively during step (2) is cultivated to be the short sex-limited Silkworm varieties of period of duration speckle, Japanese short In period of duration speckle sex-limited Silkworm varieties SGSLC, SGSLJ and step (3) after domestication and improvement in be silkworm Speckle autosexing race, Japanese pattern of silkworm autosexing race SLC-14 from, SLJ-02 from being parent, with in × in, Be made into double cross original seed day × day, in, Japanese double cross original seed hybridization obtain quaternary first cross combination, can group It is made into the double sex-limited four-place combination of robustness skin speckle, it is thus achieved that robustness is from going out the double sex-limited cultivated silkworm breed variety of cocoon speckle " SLC-14 from SGSLC × SLJ-02 from SGSLJ "；

In step (1), the principal character characteristic of described parent " SLC-13 " is: in be bivoltine four Hibernating worm kind；Larva has sex-limited speckle, and female silkworm is general speckle, and male silkworm is element speckle；Hasten the hatching of silkworms through 10 days, children The worm phase, through 19～23 days, stings middle through 14～15 days；Worm pupa rate 70%～100%；Cocoon shape is oval, cocoon Color is pure white；Cocoon weight 1.3～1.7g, Cocoon layer ratio 22%～26%；

In step (1), the principal character characteristic of described parent " SLJ-01 " is: Japanese bivoltine four Hibernating worm kind；Larva has sex-limited speckle, and female silkworm is general speckle, and male silkworm is element speckle；Hasten the hatching of silkworms through 10 days, children The worm phase, through 21～23 days, stings middle through 15～16 days；Worm pupa rate 40% 100%；Cocoon shape is girdled the waist, cocoon Color is pure white；Cocoon weight 1.4～1.8g, Cocoon layer ratio 19%～23%；

In step (1), with " SLC-13 " and " 932 " as parent, incubation is the sex-limited product of speckle The principal character characteristic of kind of " SLC-14 " is: in be bivoltine four-moulter kind；Larva has sex-limited speckle, Female silkworm is general speckle, and male silkworm is element speckle；Hasten the hatching of silkworms through 10 days, larval phase through 20～22 days, middle process of stinging 13～14 days；Worm pupa rate 80%～100%；Cocoon shape is oval, and cocoon color is pure white；Cocoon weight 1.2～1.5g, cocoon Layer rate 19%～22%；

In step (1), with " SLJ-01 " and " 7532 " as parent, the sex-limited product of Japanese speckle of incubation The principal character characteristic planting " SLJ-02 " is: Japanese bivoltine four-moulter kind；Larva has sex-limited speckle, Female silkworm is general speckle, and male silkworm is element speckle；Hasten the hatching of silkworms through 10 days, larval phase through 20～22 days, middle process of stinging 13～15 days；Worm pupa rate 80%～100%；Cocoon shape is girdled the waist, and cocoon color is pure white；Cocoon weight 1.2～1.7g, cocoon Layer rate 19%～22%；

In step (2), the principal character characteristic of described parent " SGC-01 " is: in be bivoltine four Hibernating worm kind；Larva element speckle；Hasten the hatching of silkworms through 10 days, larval phase through 17～19 days, sting middle through 12～ 13 days；Worm pupa rate 80%～100%；Cocoon shape is oval, and cocoon color is pure white；Cocoon weight 1.2～1.4g, Cocoon layer ratio 16%～18%；

In step (2), the principal character characteristic of described parent " SGJ-01 " is: Japanese bivoltine Four-moulter kind；Ovum redness of the skin or complexion；The general speckle of larva (has minority element speckle silkworm)；Hasten the hatching of silkworms through 10 days, larval phase warp Spend 20～22, sting middle through 13～14 days；Worm pupa rate 40%～100%；Cocoon shape is girdled the waist, and cocoon color is pure white； Cocoon weight 1.2～1.4g, Cocoon layer ratio 15%～17%；

In step (2), with " SLC-14 " and " SGC-01 " as parent, it incubation is short fertility The principal character characteristic of phase speckle autosexing race " SGSLC " is: in be bivoltine four-moulter kind；Larva Having sex-limited speckle, female silkworm is general speckle, and male silkworm is element speckle；Hastening the hatching of silkworms through 10 days, larval phase, is through 18～20 Day, sting middle through 12～14 days, worm pupa rate 90%～100%；Cocoon shape is oval, and cocoon color is pure white；Cocoon weight 1.1～1.7g, Cocoon layer ratio 17%～19%, certainly go out cocoon rate 90%～100%；

In step (2), with " SLJ-02 " and " SGJ-01 " as parent, the Japanese short period of duration of incubation Speckle autosexing race " SGSLJ ", because being initially separated out redness of the skin or complexion ovum and black ovum from F2 generation, two kinds of ovum colors are equal Hatching red ant and black ant, so only selecting the ovum circle hatching completely black newly-hatched silkworm in black ovum to reserve seed for planting, selecting through 6 generations Select, be black ovum, black ant to F7 generation.Its principal character characteristic is: Japanese bivoltine four-moulter kind； Larva has sex-limited speckle, and female silkworm is general speckle, and male silkworm is element speckle；Hasten the hatching of silkworms through 10 days, larval phase through 18～ 22, sting middle through 12～14 days, worm pupa rate 80%～100%；Cocoon shape is girdled the waist, and cocoon color is pure white；Whole cocoon Amount 1.2～1.5g, Cocoon layer ratio 16%～19%, certainly go out cocoon rate 90%～100%；

In step (3), described " SLC-14 from " and " SLJ-02 from " its principal character characteristic and " SLC-14 " and " SLJ-02 " is more or less the same (without significant difference), but certainly goes out cocoon rate and increase；

In step (4), " SLC-14 is from SGSLC from going out the double sex-limited cultivated silkworm breed variety of cocoon speckle for described robustness × SLJ-02 is from SGSLJ " principal character characteristic be: quaternary first cross kind, larva has sex-limited Speckle, female silkworm is general speckle, and male silkworm is element speckle；Silkworm hatching, molting, homogeneous of being placed on small straw bundles to spin cocoons, silkworm body is strong, from Going out cocoon rate and reach more than 95%, easily raise, easily breed, moultinism and diapause stable performance, integral economic trait is excellent Good；

Described integral economic trait well be preferably worm pupa rate 80%～100%, cocoon weight 1.10g～1.80g, Cocoon layer ratio 16.00%～23.00%.

The present invention has such advantages as relative to prior art and effect:

(1) the double autosexing race of the speckle that the present invention cultivates can utilize the sex-limited feature of speckle of silkworm by female in larval phase Male silkworm separately, and Pupa bombycis after cocoon internalization moth can voluntarily cocoon-break and go out, certainly going out cocoon rate reaches more than 95%, other Major Economic reaches or close to the level of commercial variety.

(2) this kind has that period of duration is short, body constitution is strong, Quality of Stored Cocoon And Silk is excellent, certainly go out cocoon rate high. Period of duration is short to be meant that and decreases with Mulberry amount and artificial making time, and larva judges that male and female are accurate soon and certainly go out The advantage of cocoon can help seed station solution labour force in great shortage and save a large amount of people expended because kind of cocoon cuts cocoon mirror pupa Power, material resources and financial resources expenditure, reduce silkworm egg production cost, can directly exempt seed station amount of labour used demand maximum Kind cocoon cut cocoon point male and female link, and ensure the male and female separation rate of 100%, reduce a large amount of of production of hybrid seeds link simultaneously Recruitment, is greatly improved hybrid seed yield and quality of silkworm eggs, thus significantly improves labor efficiency and the economy of seed station Benefit.

(3) it addition, by realize female male silkworm divide support and male and female cocoon separate filature, also can be effectively improved reeling mill Raw Silk Production and grade, significantly improve the economic benefit of reeling mill；Medicine silkworm is eaten in exploitation also to be had extensively Wealthy application prospect.

Detailed description of the invention

Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention do not limit In this.

In be existing production cultivated silkworm breed variety to be " 932 ", Japanese existing production cultivated silkworm breed variety " 7532 " There is provided by Guangdong Silkworm Spread Center that (looking after one's family, Shen Changping, Yao Fuguang, etc. silkworm summer autumn new varieties The incubation [J] of 932 Hibisci Mutabilises × Hunan sunshine 7532 (Guangdong and Guangxi Provinces two). Guangxi silkworm industry, 1995, (1): 44～48.)

" SLC-13 ", " SLJ-01 ", " SGC-01 ", " SGJ-01 " these four cultivated silkworm breed varieties are bought in Guangdong Province silkworm industry Technique Popularizing center；

Embodiment 1

(1) with man of middle system pattern of silkworm autosexing race " SLC-13 " for maternal, with in be that silkworm is used in existing production Kind " 932 " is male parent, and hybridization is speckle autosexing race " SLC-14 " in obtaining；With Japanese pattern of silkworm Autosexing race " SLJ-01 " is maternal, with Japanese existing production cultivated silkworm breed variety " 7532 " as male parent, and hybridization Obtain speckle autosexing race Japanese " SLJ-02 "；To " SLC-14 " and F1～F5 of " SLJ-02 " that obtain In generation, is respectively adopted the mixing of 0.5g ant and educates, and F6 generation begins with one batch rearing, select to have sex-limited speckle and cocoon shape, Cocoon color close relative this 932 and 7532, the good individuality of integral economic trait or moth district reserve seed for planting subculture, fixed by 17 generations To cultivation, it is bred as new family's integument speckle autosexing race " SLC-14 " and " SLJ-02 ", has possessed speckle sex-limited Feature, and integral economic trait reaches or close to producer goods kind, illustrates successfully by speckle sex limited gene Import in production Silkworm varieties.Wherein, the principal character characteristic of " SLC-14 " is: in be bivoltine four-moulter Kind；Larva has sex-limited speckle, and female silkworm is general speckle, and male silkworm is element speckle；Hasten the hatching of silkworms through 10 days, larval phase Through 21 days, sting middle through 14 days；Worm pupa rate 92.36%；Cocoon shape is oval, and cocoon color is pure white；Cocoon weight 1.42g, Cocoon layer ratio 20.33%；The principal character characteristic of " SLJ-02 " is: Japanese bivoltine four-moulter kind；Larva has Finiteness speckle, female silkworm is general speckle, and male silkworm is element speckle；Hasten the hatching of silkworms through 10 days, larval phase through 21 days, Sting middle through 14 days；Worm pupa rate 89.01%；Cocoon shape is girdled the waist, and cocoon color is pure white；Cocoon weight 1.35g, Cocoon layer ratio 19.43%.

(2) " SLC-14 " cultivated with step (1) is for maternal, and middle is short period of duration low Cocoon layer ratio product Planting " SGC-01 " is male parent, and hybridization is short period of duration speckle autosexing race " SGSLC " in obtaining；With step (1) " SLJ-02 " cultivated is female parent, children in Japanese short period of duration low Cocoon layer ratio kind " SGJ-01 " The silkworm of worm element speckle is male parent, and hybridization obtains Japanese short period of duration speckle autosexing race " SGSLJ "；To obtain In F1～the F4 generation of " SGSLC " and " SGSLJ ", is respectively adopted the mixing of 0.5g ant and educates, and selects economic characters Good individuality, uses from going out cocoon method moth appearance production of hybrid seeds subculture；In F5 generation, begins with one batch rearing, and selects economy Individuality that character is good or moth district, use and reserve seed for planting subculture, through the cultivation in 15～20 generations, respectively from going out cocoon method It is the short sex-limited Silkworm varieties of period of duration speckle and Japanese short period of duration speckle that cultivation come from cocoon rate to reach in more than 95% The sex-limited Silkworm varieties of stricture of vagina；F6 proceeds by combining ability test in early days, and the two hybridization is made into binary first cross combination " SGSLC × SGSLJ ", compares with existing production kind Guangdong and Guangxi Provinces, Guangdong two (9 Hunan, cottonrose hibiscus × 7), Analyzed by combining ability early prediction, to predict the production capacity of the double autosexing race of speckle that target is bred as；Its In, the principal character characteristic of " SGSLC " that cultivate is: in be bivoltine four-moulter kind；Larva has Finiteness speckle, female silkworm is general speckle, and male silkworm is element speckle；Hasten the hatching of silkworms through 10 days, larval phase through 19 days, Sting middle through 14 days, worm pupa rate 94.38%；Cocoon shape is oval, and cocoon color is pure white；Cocoon weight 1.52g, Cocoon layer ratio 17.97%, certainly go out cocoon rate 95.20%；Described " SGSLJ ", because being initially separated out redness of the skin or complexion ovum from F2 generation With black ovum, two kinds of ovum colors all hatching red ant and black ant, hatching completely black newly-hatched silkworm so only selecting in black ovum Ovum circle reserve seed for planting subculture.Select through 6 generations, be black ovum, black ant to F7 generation.Its principal character characteristic is: Japanese bivoltine four-moulter kind；Larva has sex-limited speckle, and female silkworm is general speckle, and male silkworm is element speckle；Hasten the hatching of silkworms Through 10 days, larval phase, through 20 days, stung middle through 14 days, worm pupa rate 85.55%；Cocoon shape is girdled the waist, cocoon Color is pure white；Cocoon weight 1.37g, Cocoon layer ratio 18.04%, certainly go out cocoon rate 97.18%.

(3) enter from going out " SLC-14 " and " SLJ-02 " that step (1) cultivated by cocoon method with Bombycis mori Row domestication and improvement (raises training under high temperature and humidity condition (temperature 25 DEG C～32 DEG C, humidity 65%～100%) The kind educated, then selects the individual employing Bombycis mori that integral economic trait is good from going out the cocoon method moth appearance production of hybrid seeds, selects Select and can certainly go out the individuality of cocoon and reserve seed for planting subculture), it is respectively designated as " SLC-14 from " and " SLJ-02 oneself "；Pass through Domestication and improvement found that, it is thus achieved that " SLC-14 from " and " SLJ-02 from " its principal character characteristic and " SLC-14 " and " SLJ-02 " is more or less the same, but certainly goes out cocoon rate and increase (being shown in Table 1).

(4) the speckle autosexing race cultivated with step (3) is SLC-14 from and step (2) cultivation The SGSLC gone out is parent, and assembling in cutification speckle sex-limited double cross original seed is " SLC-14 is from SGSLC ".With The SGSLC that the Japanese SLC-14 of speckle autosexing race oneself and the step (2) cultivated with step (3) is cultivated For parent, assemble cutification speckle sex-limited double cross original seed Japanese " SLJ-02 is from SGSLJ ", investigate its sericulture achievement And certainly go out cocoon rate, refer to table 2.Result shows, the worm pupa rate of middle system original seed and Japanese original seed is respectively 96.55%, 92.75%, certainly go out cocoon rate and be respectively 96.75%, 97.65%.

(5) be Experimental Comparison be bred as " SGSLC " and " SGSLJ " certainly go out cocoon level, the present invention divides " SLC-14 is certainly " that " SLC-14 ", " SLJ-02 " that do not obtain with step (1) and step (3) obtain " SLJ-02 is certainly " is comparison, carries out in July, 2012, in October, 2012 and in June, 2013 From going out cocoon contrast test, refer to table 1.Result shows: the cocoon rate that certainly goes out of " SGSLC " is 96.92%, than " SLC-14 ", " SLC-14 is certainly " difference high 6.29% and 4.43%；And the cocoon rate that certainly goes out of " SGSLJ " is 96.96, higher by 9.27 than " SLJ-02 ", " SLJ-02 from ", 7.55%.

(6) step (4) is assembled double cross original seed " SLC-14 is from SGSLC " and " SLJ-02 of acquisition From SGSLJ " hybridization combines the double sex-limited four-place combination of robustness skin speckle " SLC-14 is from SGSLC × SLJ-02 From SGSLJ ", compare with the existing production in Guangdong kind " 9 Hunan, cottonrose hibiscus × 7 ", carry out test of breeding silkworms, The most every economic characters with inspection SLC-14 from, SGSLC, SLJ-02 from and SGSLJ between Combining ability, thus predict the production capacity of the double autosexing race of speckle of incubation, refer to table 3.Result shows: " SLC-14 from SGSLC × SLJ-02 from SGSLJ " its full age is through the ratio short 19h of comparison kind, worm pupa Rate, a silk length, unwinding Silk Mill, reelability percentage, certainly go out cocoon rate and all exceed comparison kind, wherein reach from going out cocoon rate 98.94%, illustrate that the double autosexing race of speckle that the present invention cultivates has that period of duration is short, body constitution is strong, silk product Of fine quality good, certainly go out cocoon rate high.

The Bombycis mori of each kind of table 1 is from going out cocoon rate

The sericulture achievement of table 2 skin speckle sex-limited double cross original seed

Table 3 " SLC-14 from SGSLC × SLJ-02 from SGSLJ " (reciprocal crosses) laboratory qualification achievement

Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not by above-mentioned reality Execute the restriction of example, the change made under other any spirit without departing from the present invention and principle, modification, Substitute, combine, simplify, all should be the substitute mode of equivalence, within being included in protection scope of the present invention.

Claims (10)

1. a robustness is from the breeding method going out the double sex-limited cultivated silkworm breed variety of cocoon speckle, it is characterised in that comprise as Lower step:

(1) respectively with middle system, Japanese pattern of silkworm autosexing race as female parent, with middle system, Japanese existing production Being male parent with cultivated silkworm breed variety, hybridization obtains new man of middle system pattern of silkworm autosexing race and new Japanese silkworm respectively Speckle autosexing race；New man of middle system pattern of silkworm autosexing race and Japanese new pattern of silkworm to obtaining are sex-limited In F1～the F5 generation of kind, is respectively adopted 0.2～1.0g ant amount mixing and educates, and F6 generation begins with one batch rearing, selects There is the good individuality of sex-limited speckle and cocoon shape, the nearly male parent of cocoon color, integral economic trait or reserve seed for planting selection-breeding in moth district, Cultivate by many possess the sex-limited feature of speckle for directive breeding, and integral economic trait reaches or close life The new speckle autosexing race of product kind；

(2) respectively with new man of the middle system pattern of silkworm autosexing race cultivated in step (1), new Japanese Family pattern of silkworm autosexing race be female parent, with in be short period of duration low Cocoon layer ratio kind, the Japanese low cocoon of short period of duration Layer rate kind is male parent, and hybridization is short period of duration speckle autosexing race and Japanese short period of duration speckle limit in obtaining Property kind；To obtain in be short period of duration speckle autosexing race and Japanese short period of duration speckle autosexing race In F1～F4 generation, is respectively adopted 0.2～1.0g ant amount mixing and educates, and selects the individuality that integral economic trait is good, uses Bombycis mori is from the method moth appearance production of hybrid seeds going out cocoon；In F5 generation, begins with one batch rearing, and selects integral economic trait good Individual or moth district, uses Bombycis mori to reserve seed for planting selection-breeding from the method going out cocoon；Selected above through 7 generations, cultivate respectively Come from cocoon rate reaching in more than 95% is the short sex-limited Silkworm varieties of period of duration speckle and Japanese short period of duration speckle limit Property Silkworm varieties；

(3) new man of the middle system pattern of silkworm using Bombycis mori to cultivate step (1) from the method going out cocoon is sex-limited Kind, Japanese pattern of silkworm autosexing race proceed by domestication and improvement from F16 so that it is certainly go out cocoon rate and improve extremely More than 95%, it is thus achieved that man of the middle system pattern of silkworm autosexing race after domestication and improvement, Japanese pattern of silkworm autosexing race；

(4) respectively during step (2) is cultivated to be the short sex-limited Silkworm varieties of period of duration speckle, Japanese short Man of middle system pattern of silkworm autosexing race after domestication and improvement, day in the sex-limited Silkworm varieties of period of duration speckle and step (3) Be family's pattern of silkworm autosexing race be parent, with in × in, day × day be made into double cross original seed, in, Japanese double cross Original seed hybridization obtains quaternary first cross combination, is robustness from going out the double sex-limited cultivated silkworm breed variety of cocoon speckle.

Robustness the most according to claim 1 goes out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle certainly, It is characterized in that:

Described in step (1) is that man of middle system pattern of silkworm is sex-limited as maternal man of middle system pattern of silkworm autosexing race Kind " SLC-13 ".

Robustness the most according to claim 1 goes out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle certainly, It is characterized in that:

Described in step (1) is that Japanese pattern of silkworm is sex-limited as Japanese maternal pattern of silkworm autosexing race Kind " SLJ-01 ".

Robustness the most according to claim 1 goes out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle certainly, It is characterized in that:

Described in step (1) as male parent is during existing production cultivated silkworm breed variety is be existing produce use Cultivated silkworm breed variety " 932 ".

Robustness the most according to claim 1 goes out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle certainly, It is characterized in that:

The Japanese existing production cultivated silkworm breed variety as male parent described in step (1) is that Japanese existing production is used Cultivated silkworm breed variety " 7532 ".

Robustness the most according to claim 1 goes out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle certainly, It is characterized in that:

Described in step (2) as male parent being be short period of duration during short period of duration low Cocoon layer ratio kind is Low Cocoon layer ratio kind " SGC-01 ".

Robustness the most according to claim 1 goes out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle certainly, It is characterized in that:

The Japanese short period of duration low Cocoon layer ratio kind as male parent described in step (2) is Japanese short period of duration Low Cocoon layer ratio kind " SGJ-01 ".

Robustness the most according to claim 1 goes out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle certainly, It is characterized in that:

Integral economic trait described in step (1), (2) is well worm pupa rate 60%～100%, cocoon weight 1.00g～2.00g, Cocoon layer ratio 13.00%～28.00%.

Robustness the most according to claim 1 goes out the breeding method of the double sex-limited cultivated silkworm breed variety of cocoon speckle certainly, It is characterized in that:

Domestication and improvement described in step (3) refers to raise the kind of cultivation under the conditions of high temperature and humidity, then Select the individual method moth appearance production of hybrid seeds using Bombycis mori certainly to go out cocoon that integral economic trait is good, select certainly go out cocoon Individuality is reserved seed for planting subculture；

Described high temperature and humidity condition is temperature 25 DEG C～32 DEG C, humidity 65%～100%.

10. the robustness described in any one of claim 1～9 is from the cultivation going out the double sex-limited cultivated silkworm breed variety of cocoon speckle Method application in silkworm improved seeds cultivation field.