CN107318790A - A kind of breeding method of the sex-limited cultivated silkworm breed variety of yellowish green cocoon of robustness and application - Google Patents
A kind of breeding method of the sex-limited cultivated silkworm breed variety of yellowish green cocoon of robustness and application Download PDFInfo
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Abstract
The present invention discloses breeding method and the application of a kind of sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness.The present invention is successfully realized the cultivation of the sex-limited cultivated silkworm breed variety of yellowish green cocoon first, the kind can predict female ratio in advance in parent silkworm rearing, cocoon mirror pupa recruitment is cut in saving, reduce silkworm egg production cost, and ensure 100% male and female separation rate, a large amount of recruitments of production of hybrid seeds link are reduced simultaneously, hybrid seed yield and quality of silkworm eggs are greatly improved, so as to significantly improve efficiency and economic benefit；And can also realize that male and female cocoon separates filature, is utilized respectively on raw cocoon production, raw silk value can be effectively improved, the Raw Silk Production and grade of reeling mill is improved, economic benefit is significantly improved.The yellow silk and green silk of the yellow green cocoon institute reel silk from cocoons of the kind are natural colorful silk, the functions such as its moisturizing having, warming, antibacterial, anti-oxidant and masking ultraviolet are better than the white silk of silkworm, the characteristics of with natural, green, low-carbon, its exploitation meets Current resource saving, environment-friendly idea of development.
Description
Technical field
Field is cultivated the invention belongs to the excellent characteristic Silkworm varieties of silkworm, and in particular to a kind of sex-limited silkworm of yellowish green cocoon of robustness
The breeding method of kind and application.
Background technology
Natural colorful silk has the functions such as moisturizing, warming, antibacterial, anti-oxidant and masking ultraviolet white better than silkworm simultaneously
Color silk, natural colorful cocoon resource is the primary kind of nature, the characteristics of with " natural, green, low-carbon ", and it is developed and met
Currently the idea of development of " resources conservation, environment-friendly ", there is very wide DEVELOPMENT PROSPECT.The sex-limited germplasm of cocoon color is to use
The female pupa of 60Cor radiation exposure silkworms, makes yellow blood group translocation on its autosome to W chromosomes, obtains sex-limited cocoon mutation
System, the female silkworm knot Yellow Cocoon being bred as based on this, the white cocoon of male silkworm knot.The research of cocoon color autosexing race is in order in parent silkworm rearing
In predict female ratio in advance, cocoon mirror pupa recruitment is cut in saving, reduces silkworm egg production cost；And male and female can be also realized on raw cocoon production
Cocoon is utilized respectively, and improves raw silk value, carries out the research of cocoon color autosexing race, to significantly improving silkworm raiser and silk enterprise
Economic benefit has important practical significance.At present, it is domestic also without practical sex-limited (yellowish-white cocoon color the is sex-limited) silkworm of cocoon color of energy
Kind, the incubation of the sex-limited Silkworm varieties of yellow, green cocoon has not been reported, therefore, carries out the sex-limited silkworm breeding material of yellow, the green cocoon of robustness
Material innovation, to accelerating the diversified of Guangdong production Silkworm varieties, silk product superior, adapting to silk product structural adjustment has
Very big effect.
The content of the invention
In order to overcome the shortcoming and deficiency of prior art, it is an object of the invention to provide a kind of yellowish green cocoon of robustness is sex-limited
The breeding method of cultivated silkworm breed variety.
Another object of the present invention is to provide the application of above-mentioned breeding method.
The purpose of the present invention is achieved through the following technical solutions：
A kind of breeding method of the sex-limited cultivated silkworm breed variety of yellowish green cocoon of robustness, comprises the following steps：
(1) using Japanese chromatic cocoon autosexing race as female parent, respectively with the green cocoon kind of middle system, middle system's integral economic trait into
The good white cocoon kind of achievement and Japanese existing production are male parent with white cocoon kind, are Cocoon color during hybridization is obtained newly respectively
Autosexing race and new Japanese Cocoon color autosexing race；To acquisition it is new in be Cocoon color autosexing race and new Japanese
The F1 generation of Cocoon color autosexing race is respectively adopted the mixing of 0.2~1.0g ants and educated, and F2 generations start to use one batch rearing, and selection has spot
Seed selection that line is unified, the yellow cocoon of knot and the good individual of green cocoon, integral economic trait or moth area reserve seed for planting, by for directive breeding, cultivating more
The standby sex-limited feature of yellow green is provided, i.e., yellow cocoon is female, and green cocoon is male, and integral economic trait reaches or close to the new of commercial variety
Cocoon color autosexing race, so as to realize the cultivation purpose of the sex-limited commercial variety of yellow green；
(2) to be silkworm in being obtained in step (1) by the green cocoon mixing breed of Japanese chromatic cocoon autosexing race and middle system
Cocoon color autosexing race is female parent, using the white cocoon kind of the good practicality of middle system's integral economic trait achievement as male parent, and hybridization is obtained
It is new in be cocoon color autosexing race；To acquisition it is new in be cocoon color autosexing race respectively with general spot, knot yellow green cocoon and element
Seed selection is reserved seed for planting subculture respectively for spot, knot yellow green cocoon two kinds of Breed dirctions；
(3) obtained with passing through Japanese chromatic cocoon autosexing race and Japanese existing produce in step (1) with white cocoon mixing breed
Japanese Cocoon color autosexing race for female parent, then using it is Japanese it is existing production with white cocoon kind as male parent, hybridization obtain newly it is Japanese
Cocoon color autosexing race；Plain spot silkworm, the individual of knot yellow green cocoon or moth area are selected and remain to the new Japanese cocoon color autosexing race of acquisition
Reserve seed for planting subculture, until homozygosis is plain spot silkworm, full yellow green cocoon；
(4) to being cultivated in step (1), (2), (3) in Japanese cocoon color autosexing race focus on form in seed selection early stage
The M8003 line of shape gene and fixation, the middle and later periods focus on the stable raising of quantitative character；Complete homozygosis is limited into yellow green cocoon
Property the Japanese chromatic cocoon autosexing race of kind and parent supported with batch examination and compare, excellent Sino-Japan of selection integral economic trait
It is that yellowish green cocoon autosexing race is parent, carries out hybridization and combine binary first cross combination, carry out laboratory qualification evaluation, be
The sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness.
Described in step (1) is preferably Japanese chromatic cocoon autosexing race as maternal Japanese chromatic cocoon autosexing race
“CLJ-02”；
The green cocoon kind of the middle system as male parent described in step (1) is preferably the green cocoon kind " CCC-04 " of middle system；
Described in step (1) as being that the good white cocoon kind of integral economic trait achievement is preferably middle system in male parent
The good white cocoon kind " RC-24 " of integral economic trait achievement；
Described in step (1), (3) is preferably Japanese existing production as Japanese existing produce with white cocoon kind of male parent
With white cocoon kind " Hunan sunshine "；
Described in step (2) as being that the white cocoon kind of the good practicality of integral economic trait achievement is preferred in male parent
For the white cocoon kind " Feng Xuan " of the good practicality of middle system's integral economic trait achievement；
The unified preferably unified general spot of selection of speckle or unified selectin spot described in step (1)；
Integral economic trait achievement described in step (1), (2) is preferably well worm pupa rate 60%~100%, cocoon weight
1.00g~2.00g, Cocoon layer ratio 13.00%~28.00%；
Seed selection early stage described in step (4) focuses on the M8003 line and fixed preferably F1~F15 of morphological characters gene
In generation, is emphatically according to general spot, the yellowish green cocoon of knot and plain spot, the yellowish green two kinds of direction seed selections of cocoon of knot, emphatically speckle, cocoon color, the selection of cocoon shape；
Stable improve that the described middle and later periods focuses on quantitative character is preferably F15 generations later worm pupa rate, cocoon weight, cocoon shell weight, cocoon emphatically
The stable raising of the economic characters such as layer rate；The vitality of cultivation kind is set to reach or close in fact to realize preferably through more for seed selection
With the target of varietal level；
The breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of described robustness, preferably comprises following steps：
(1) be female parent with Japanese chromatic cocoon autosexing race " CLJ-02 ", respectively with the green cocoon kind " CCC-04 " of middle system, in
It is the good white cocoon kind " RC-24 " of integral economic trait achievement, Japanese existing production is male parent with white cocoon kind " Hunan sunshine ", point
It is Cocoon color autosexing race " CLJ-02 × CCC-04 " (referred to as " CLC-03 "), " CLJ-02 × RC- in Za Jiao not obtaining newly
24 " (referred to as " CLC-04 ") and Japanese Cocoon color autosexing race " CLJ-02 × Hunan sunshine " (referred to as " CLJ-06 "), to acquisition
" CLC-03 ", " CLC-04 " and " CLJ-06 " F1 generation are respectively adopted the mixing of 0.5g ants and educate raising, and F2 generations start to use one batch rearing
Raise.
(2) it is that the F1 generation of Cocoon color autosexing race " CLC-04 " is yellowish green for general spot, knot during hybridization is obtained in step (1)
Bai Jian, selects and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserves seed for planting subculture, and F2 is for isolating plain spot silkworm, tie yellowish green white in general spot
Cocoon, selects and remain plain spot silkworm, yellow green cocoon subculture, for homozygosis is plain spot silkworm, full yellow green cocoon to F7；
(3) it is that the F1 generation of Cocoon color autosexing race " CLC-03 " is yellowish green for general spot, knot during hybridization is obtained in step (1)
In Bai Jian, continuous 6 generation, selects and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserves seed for planting subculture, for homozygosis is general spot silkworm to F7, complete yellowish green
Color cocoon；
(4) be in being obtained using hybridization in step (1) F1 generation of Cocoon color autosexing race " CLC-03 " to be maternal, middle system
The white cocoon kind " Feng Xuan " of the good practicality of integral economic trait achievement is male parent, is cocoon color autosexing race during hybridization acquisition is new
" CLC-03 × Feng Xuan ", " CLC-03 × Feng Xuan " F1 generations are general spot, tie yellowish green white cocoon, and F2 is yellowish green white on behalf of general spot, plain spot, knot
Cocoon, selects and remain general spot, the individual of knot yellow green cocoon or moth area and reserves seed for planting subculture, be named as " CLC-05 ", select and remain plain spot, knot Yellow Cocoon
Individual or moth area reserve seed for planting subculture, be named as " CLC-06 ".
(5) " CLC-05 " one obtained in step (4) reserves seed for planting until selecting and remain general spot, the individual of knot yellow green cocoon or moth area
Subculture, for homozygosis is general spot silkworm to F5, but ties yellowish green white cocoon, for homozygosis is general spot silkworm, full yellow green cocoon to F10；Obtain
" CLC-06 " then selects and remain plain spot, the individual of knot yellow green cocoon or moth area and reserved seed for planting subculture always, is plain spot silkworm, knot to F5 band homozygosis
Yellowish green white cocoon, but do not go out homozygosis also for cocoon color to F22, still ties that yellow, powder is yellow, green, white cocoon.
(6) F1 generation for the Japanese Cocoon color autosexing race " CLJ-06 " that hybridization is obtained is general spot, ties yellowish green in step (1)
Bai Jian, selects and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserves seed for planting subculture, and F2 is for isolating plain spot silkworm, tie yellowish green white in general spot
Cocoon, selects and remain plain spot silkworm, yellow green cocoon subculture, for homozygosis is plain spot silkworm, full yellow green cocoon to F7；
(7) it is female parent to obtain Japanese silkworm autosexing race " CLJ-06 " F3 generations (plain spot silkworm) in step (6), then with Japanese
Existing production is male parent with white cocoon kind " Hunan sunshine ", and hybridization obtains new Japanese cocoon color autosexing race " CLJ-06 × Hunan sunshine " (letter
Claim " CLJ-07 "), select and remain plain spot silkworm, the individual of knot yellow green cocoon or moth area and reserve seed for planting subculture, to F13 for homozygosis be plain spot silkworm,
Full yellow green cocoon.
(8) to be in being cultivated in step (2), (3), (5), (6), (7) Cocoon color autosexing race " CLC-03 ",
" CLC-04 ", " CLC-05 ", " CLC-06 " and Japanese Cocoon color autosexing race " CLJ-06 ", " CLJ-07 " this 6 kinds exist
Seed selection early stage focuses on M8003 line and the fixation of morphological characters gene, and the middle and later periods focuses on the stable raising of quantitative character.To 5
Complete homozygosis, which into the sex-limited kind of yellow green cocoon and parent " CLJ-02 " support with batch examination, to be compared, and selects integral economic trait
Excellent middle system " CLC-04 ", Japanese " CLJ-06 ", carry out hybridization and combine binary first cross combination, carry out laboratory qualification
Evaluate, obtain the sex-limited cultivated silkworm breed variety " CLC-04 × CLJ-06 " of the yellowish green cocoon of robustness.
In step (1), the principal character characteristic of described parent " CLJ-02 " is：Japanese bivoltine four-moulter kind,
Larva body spot is general spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 21~22, sting middle pass through 14~15；Worm pupa rate 65%~
100%；Cocoon shape is girdled the waist, and yellowish green (a small number of white cocoons) cocoon is sex-limited, and yellow cocoon is female, and green, white cocoon is hero；1.4~1.8g of cocoon weight, cocoon layer
Rate 17%~20%；
In step (1), the principal character characteristic of described parent " CCC-04 " is：Middle system's bivoltine four-moulter kind,
Larva body spot is general spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 19~20, sting middle pass through 12~13；Worm pupa rate 70%~
100%；Cocoon shape is oval, and cocoon green (has the white cocoon of only a few), 1.1~1.5g of cocoon weight, Cocoon layer ratio 14%~17%；
In step (1), the principal character characteristic of described parent " RC-24 " is：Middle system's bivoltine four-moulter kind；Children
Polypide spot is plain spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 21~23, sting middle pass through 14~16；Worm pupa rate 70%~
100%；Cocoon shape is oval, and cocoon color is pure white；1.4~1.8g of cocoon weight, Cocoon layer ratio 22%~26%；
It is Cocoon color autosexing race in incubation with " CLJ-02 " and " CCC-04 " for parent in step (1)
The principal character characteristic of " CLC-03 " is：Middle system's bivoltine four-moulter kind；Larva body spot is general spot；Hasten the hatching of silkworms by 10 days, children
The worm phase passes through 19~21, stings middle pass through 14~16；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow
Cocoon is that female, green cocoon is male；1.4~1.8g of cocoon weight, Cocoon layer ratio 16%~20%；
It is Cocoon color autosexing race " CLC- in incubation with " CLJ-02 " and " RC-24 " for parent in step (1)
04 " principal character characteristic is：Middle system's bivoltine four-moulter kind；Larva body spot is plain spot；Hasten the hatching of silkworms by 10 days, larval phase warp
19~21 are spent, is stung middle pass through 13~15；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow cocoon be it is female,
Green cocoon is hero；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；
In step (1), with " CLJ-02 " and " Hunan sunshine " for parent, the Japanese Cocoon color autosexing race " CLJ- of incubation
06 " principal character characteristic is：Japanese bivoltine four-moulter kind；Larva body spot is plain spot；Hasten the hatching of silkworms by 10 days, larval phase warp
20~22 are spent, is stung middle pass through 13~15；Worm pupa rate 80%~100%；Cocoon shape is girdled the waist, and yellowish green cocoon is sex-limited, i.e., yellow cocoon be it is female,
Green cocoon is hero；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；
It is cocoon color autosexing race " CLC-05 " in incubation with " CLC-03 " and " Feng Xuan " for parent in step (4)
Principal character characteristic is：Middle system's bivoltine four-moulter kind；Larva body spot is general spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 20
~22, sting middle pass through 13~15；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow cocoon is female, green cocoon
For hero；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；It is the main spy of cocoon color autosexing race " CLC-06 " in being bred as
Intrinsic characteristics is：Middle system's bivoltine four-moulter kind；Larva body spot is plain spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 20~22,
Sting middle pass through 13~15；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green white cocoon is sex-limited, i.e., yellow cocoon is female, and green, white cocoon is
It is male；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；
In step (7), with " CLJ-06 " and " Hunan sunshine " for parent, the Japanese Cocoon color autosexing race " CLJ- of incubation
07 " principal character characteristic is：Japanese bivoltine four-moulter kind；Larva body spot is plain spot；Hasten the hatching of silkworms by 10 days, larval phase warp
20~22 are spent, is stung middle pass through 14~16；Worm pupa rate 75%~100%；Cocoon shape is girdled the waist, and yellowish green cocoon is sex-limited, i.e., yellow cocoon be it is female,
Green cocoon is hero；1.4~1.9g of cocoon weight, Cocoon layer ratio 18%~22%.
In step (8), the yellowish green cocoon of described robustness sex-limited cultivated silkworm breed variety " CLC-04 × CLJ-06 " principal character
Characteristic is：Binary first cross kind, larva body spot is plain spot；Silkworm hatching, molting, homogeneous of being placed on small straw bundles to spin cocoons, cocoon color are bright-coloured, yellowish green cocoon
Sex-limited, i.e., Yellow Cocoon is female, and green cocoon is hero；Silkworm body is strong, easily raises, easily breeds, diapause performance is stable, integral economic trait
It is excellent；
Excellent described integral economic trait is preferably worm pupa rate 80%~100%, cocoon weight 1.30g~2.2g, cocoon layer
Rate 16.00%~24.00%.
The breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of described robustness answering in silkworm improved seeds cultivation field
With.
The present invention has the following advantages and effect relative to prior art：
(1) present invention is successfully realized the cultivation of the sex-limited cultivated silkworm breed variety of yellowish green cocoon first, and the kind can utilize cocoon in pupa time
Color it is different female is separated, developmental duration, robustness, cocoon quality achievement in various degree be better than the sex-limited mesh of yellowish green (white) color cocoon
Gene parent CLJ-02 is marked, and other Major Economics reach or close to the level of commercial variety.
(2) kind can predict female ratio in advance in parent silkworm rearing, and cocoon mirror pupa recruitment is cut in saving, and reduction silkworm egg is produced into
This, and ensure 100% male and female separation rate, while reducing a large amount of recruitments of production of hybrid seeds link, greatly improve hybrid seed yield and silkworm egg
Quality, so as to significantly improve the efficiency and economic benefit of seed station；And male and female cocoon can be also realized on raw cocoon production and separates reel silk from cocoons
Silk, is utilized respectively, and can effectively improve raw silk value, improves the Raw Silk Production and grade of reeling mill, significantly improves reeling mill
Economic benefit.
(3) the yellow silk and green silk of the yellow green cocoon institute reel silk from cocoons of the kind are natural colorful silk, its moisturizing having,
The functions such as warming, antibacterial, anti-oxidant and masking ultraviolet are better than the white silk of silkworm, the spy with " natural, green, low-carbon "
Point, it develops the idea of development for meeting current " resources conservation, environment-friendly ".
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
The white cocoon kind " Feng Xuan " of the good practicality of middle system's integral economic trait achievement is in Guangdong Province's sericulture Technique Popularizing
(Guo Dingguo, Lin Jianrong, the yellow concubine of an emperor wait incubation [J] sericultures of new silkworm varieties Guangdong maple No. three (five choosings × 2832) for heart offer
Science, 2007,33 (3):466~469.)
Japanese existing production provided with white cocoon kind " Hunan sunshine " by Guangdong Silkworm Spread Center (look after one's family, Shen Chang
Flat, Yao Fuguang waits incubation [J] the Guangxi silkworm of 932 lotuses of silkworm summer and autumn new varieties × Hunan sunshine 7532 (Guangdong and Guangxi Provinces two)
Industry, 1995, (1):44~48.)
" CLJ-02 ", " CCC-04 ", " RC-24 " these three cultivated silkworm breed varieties are bought in Guangdong Silkworm Spread Center.
Embodiment 1
(1) 2005 year autumn started to be respectively female parent with Japanese chromatic cocoon autosexing race " CLJ-02 ", with the green cocoon product of middle system
The good white cocoon kind " RC-24 " of kind of " CCC-04 ", middle system's integral economic trait achievement, Japanese existing production is with white cocoon kind
" Hunan sunshine " is male parent, is Cocoon color autosexing race " CLJ-02 × CCC-04 " (referred to as " CLC- during hybridization is obtained newly respectively
03 "), " CLJ-02 × RC-24 " (referred to as " CLC-04 ") and Japanese Cocoon color autosexing race " CLJ-02 × Hunan sunshine " are (referred to as
" CLJ-06 "), the mixing of 0.5g ants is respectively adopted to the F1 generation of " CLC-03 ", " CLC-04 " and " CLJ-06 " of acquisition and educates raising,
In F2 generations, start to raise using one batch rearing, wherein, it is Cocoon color autosexing race " CLC-04 " in hybridization acquisition, F1 generation is general
Spot, tie yellowish green white cocoon, select and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserve seed for planting subculture, F2 for isolated in general spot plain spot silkworm,
Yellowish green white cocoon is tied, plain spot silkworm, yellow green cocoon subculture is selected and remain, for homozygosis is plain spot silkworm, full yellow green cocoon to F7；The F1 of " CLC-03 "
On behalf of general spot, tie yellowish green white cocoon, continuous 6 generation selects and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserved seed for planting subculture, to F7 for homozygosis
For general spot silkworm, full yellow green cocoon；The F1 generation of Japanese Cocoon color autosexing race " CLJ-06 " is general spot, ties yellowish green white cocoon, is selected and remain
General spot silkworm, the individual of yellow green cocoon or moth area reserve seed for planting subculture, and F2 selects and remain element for isolating plain spot silkworm in general spot, tying yellowish green white cocoon
Spot silkworm, yellow green cocoon subculture, for homozygosis are plain spot silkworm, full yellow green cocoon to F7.Wherein, the principal character characteristic of " CLJ-02 "
For：Japanese bivoltine four-moulter kind, larva body spot is general spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 21~22, middle warp of stinging
Spend 14~15；Worm pupa rate 65%~100%；Cocoon shape is girdled the waist, and yellowish green (a small number of white cocoons) cocoon is sex-limited, and yellow cocoon is female, and green, white cocoon is
It is male；1.4~1.8g of cocoon weight, Cocoon layer ratio 17%~20%；The principal character characteristic of " CCC-04 " is：Middle system's bivoltine four-moulter
Kind, larva body spot is general spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 19~20, sting middle pass through 12~13；Worm pupa rate
70%~100%；Cocoon shape is oval, and cocoon green (has the white cocoon of only a few), 1.1~1.5g of cocoon weight, Cocoon layer ratio 14%~17%；
The principal character characteristic of " RC-24 " is：Middle system's bivoltine four-moulter kind；Larva body spot is plain spot；Hasten the hatching of silkworms by 10 days, larva
Phase passes through 21~23, stings middle pass through 14~16；Worm pupa rate 70%~100%；Cocoon shape is oval, and cocoon color is pure white；Cocoon weight 1.4
~1.8g, Cocoon layer ratio 22%~26%.
(2) hybridized using step (1) in obtaining be the F1 generation of Cocoon color autosexing race " CLC-03 " to be maternal, middle assemblage
It is male parent to close the good white cocoon kind " Feng Xuan " of practicality of economic characters achievement, is cocoon color autosexing race during hybridization acquisition is new
" CLC-03 × Feng Xuan ", " CLC-03 × Feng Xuan " F1 generation is general spot, ties yellowish green white cocoon, and F2 is yellowish green white on behalf of general spot, plain spot, knot
Cocoon, selects and remain general spot, the individual of knot yellow green cocoon or moth area and reserves seed for planting subculture, be named as " CLC-05 ", select and remain plain spot, knot Yellow Cocoon
Individual or moth area reserve seed for planting subculture, be named as " CLC-06 ".Wherein, " CLC-05 " one is until select and remain general spot, knot yellow green cocoon
Individual or moth area reserve seed for planting subculture, for homozygosis be general spot silkworm to F5, but tie yellowish green white cocoon, to F10 for homozygosis be general spot silkworm, complete
Yellow green cocoon；" CLC-06 " obtained then selects and remain plain spot, the individual of knot yellow green cocoon or moth area and reserved seed for planting subculture always, to F5 bands
Homozygosis is plain spot silkworm, ties yellowish green white cocoon, but does not go out homozygosis also for cocoon color to F22, still ties that yellow, powder is yellow, green, white cocoon.
(3) it is female parent with the F3 generations (plain spot silkworm) of the Japanese Cocoon color autosexing race " CLJ-06 " obtained in step (1),
Again with it is Japanese it is existing production with white cocoon kind " Hunan sunshine " be male parent, hybridization obtain newly Japanese Cocoon color autosexing race " CLJ-
06 × Hunan sunshine " (referred to as " CLJ-07 "), selects and remain plain spot silkworm, the individual of knot yellow green cocoon or moth area and reserves seed for planting subculture, to F13 for pure
It is combined into plain spot silkworm, full yellow green cocoon.
(4) to be in being cultivated in step (1), (2), (3) Cocoon color autosexing race " CLC-03 ", " CLC-04 ",
" CLC-05 ", " CLC-06 " and Japanese Cocoon color autosexing race " CLJ-06 ", " CLJ-07 " this 6 kinds in seed selection early stage
The M8003 line of weight morphological characters gene and fixation, the middle and later periods focus on the stable raising of quantitative character.By the seed selection of more than 10 years,
In the Japanese kind being respectively bred as seed selection generation more than 20, at present, form and Major Economic achievement are successively stable.Wherein,
The principal character characteristic of " CLC-03 " is：Middle system's bivoltine four-moulter kind；Larva body spot is general spot；Hasten the hatching of silkworms by 10 days, children
The worm phase passes through 19~21, stings middle pass through 14~16；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow
Cocoon is that female, green cocoon is male；1.4~1.8g of cocoon weight, Cocoon layer ratio 16%~20%；The principal character characteristic of " CLC-04 " is：In
It is bivoltine four-moulter kind；Larva body spot is plain spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 19~21, passes through 13 in stinging
~15 days；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow cocoon is that female, green cocoon is male；Cocoon weight 1.5~
1.9g, Cocoon layer ratio 19%~22%；The principal character characteristic of " CLC-05 " is：Middle system's bivoltine four-moulter kind；Larva body spot
For general spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 20~22, sting middle pass through 13~15；Worm pupa rate 80%~100%；Cocoon
Shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow cocoon is that female, green cocoon is male；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；“CLC-
06 " principal character characteristic is：Middle system's bivoltine four-moulter kind；Larva body spot is plain spot；Hasten the hatching of silkworms by 10 days, larval phase warp
20~22 are spent, is stung middle pass through 13~15；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green white cocoon is sex-limited, i.e., yellow cocoon is
Female, green, white cocoon is hero；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；The principal character characteristic of " CLJ-06 " is：It is Japanese
Bivoltine four-moulter kind；Larva body spot is plain spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 20~22, middle process 13 of stinging~
15 days；Worm pupa rate 80%~100%；Cocoon shape is girdled the waist, and yellowish green cocoon is sex-limited, i.e., yellow cocoon is that female, green cocoon is male；Cocoon weight 1.5~
1.9g, Cocoon layer ratio 19%~22%.The principal character characteristic of " CLJ-07 " is：Japanese bivoltine four-moulter kind；Larva body spot
For plain spot；Hasten the hatching of silkworms by 10 days, larval phase passes through 20~22, sting middle pass through 14~16；Worm pupa rate 75%~100%；Cocoon
Pencil of forms waist, yellowish green cocoon is sex-limited, i.e., yellow cocoon is that female, green cocoon is male；1.4~1.9g of cocoon weight, Cocoon layer ratio 18%~22%.
(5) to 5 complete homozygosis carry out supporting ratio with batch examination into the sex-limited kind of yellow green cocoon and parent " CLJ-02 "
Compared with as a result showing：The developmental durations of 5 yellow green cocoon autosexing races, robustness, cocoon quality achievement in various degree better than yellowish green
The sex-limited target gene parent " CLJ-02 " of (white) color cocoon.Wherein, most prominent is middle system " CLC-04 ", Japanese " CLJ-06 ", 5 ages
Through too short 1 day, full age, full period was through too short 3 days through too short 3 days；22.45 percentage points and 10.88 hundred are respectively increased in robustness
Branch；Cocoon weight increases, and Cocoon layer ratio improves 1.67 percentage points and 0.79 percentage point (referring to table 1).
(6) according to the property stability and integral economic trait performance for newly creating each sex-limited breeding material of yellow green cocoon,
Selection is with the excellent middle system " CLC-04 " of integral economic trait and Japanese " CLJ-06 " for parent, and it is miscellaneous that hybridization combines a binary generation
Hand over combination to carry out laboratory qualification evaluation, compared with existing production kind Guangdong and Guangxi Provinces two, Guangdong.As a result show：Yellowish green cocoon cocoon color
" CLC-04 × CLJ-06 " (reciprocal cross) principal character characteristic is for sex-limited first cross combination：Hatch molting homogeneous, food mulberry is fast,
Silkworm body is elongated, full element spot silkworm；It is aging to gush together, bury cage soon, cocoon shape is neat and well spaced, and cocoon color is bright-coloured, Yellow Cocoon is female, and green cocoon is hero；Entirely
Age passes through 20 days or so, and 5 ages passed through 5 days half；Worm pupa rate 93.32% is No. two lower 1.12 percentage points than Guangdong and Guangxi Provinces；Ten thousand silkworms receive cocoon
13.79% and 15.80% is respectively increased in amount, ten thousand silkworm cocoon shell weights；Can male and female cocoon difference filature, with the male and female silk union ratio of Guangdong and Guangxi Provinces two
Compared with its male silk of cocoon one improves 17.00% up to 1246 meters, and size of cocoon filament 2.078dtex is thin by 15.74%, light folding 243Kg reductions
7.00%, 93.50 points of cleanliness improves 3.89%, and dry hair cocoon silk production rate 38.65% improves 6.39% (referring to 2~table of table 3).
The newly 6 Yellow Cocoon autosexing race subculture major traits achievement investigation of wound of table 1
" the average identification achievement (making for 2014 2,6) in the batch laboratories of CLC-04 × CLJ-06 " (reciprocal cross) 2 of table 2
" silk quality in the batch laboratories of CLC-04 × CLJ-06 " (reciprocal cross) 2 averagely identifies achievement (making for 2014 2,6) to table 3
Only 2 make the detection of sample presentation cocoon filature, cocoon rate of getting on the bus in table, silk length, reelability percentage, length of non broken filament, Cocoon Silk Weight, fiber number,
Light folding, clean, dry hair cocoon silk production rate are identified by sericulture there product quality supervision and inspection test center of the Ministry of Agriculture (Zhenjiang).CLC-
04 × CLJ-06 points female (Huang) male (green) cocoon examines silk quality respectively, and Guangdong and Guangxi Provinces two (CK) are that male and female cocoon mixes inspection.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. the breeding method of the sex-limited cultivated silkworm breed variety of a kind of yellowish green cocoon of robustness, it is characterised in that comprise the following steps：
(1) it is good with the green cocoon kind of middle system, middle system's integral economic trait achievement respectively using Japanese chromatic cocoon autosexing race as female parent
Good white cocoon kind and Japanese existing production are male parent with white cocoon kind, are that Cocoon color is sex-limited during hybridization is obtained newly respectively
Kind and new Japanese Cocoon color autosexing race；To acquisition it is new in be Cocoon color autosexing race and new Japanese silkworm
The F1 generation of cocoon color autosexing race is respectively adopted the mixing of 0.2~1.0g ants and educated, and F2 generations start to use one batch rearing, and selection has speckle system
First, tie yellow cocoon and the good individual of green cocoon, integral economic trait or moth area reserves seed for planting seed selection, by the way that for directive breeding, cultivation is provided more
The standby sex-limited feature of yellow green, i.e., yellow cocoon is female, and green cocoon is male, and integral economic trait reaches or close to the new cocoon of commercial variety
Color autosexing race, so as to realize the cultivation purpose of the sex-limited commercial variety of yellow green；
(2) to be Cocoon color in being obtained in step (1) by the green cocoon mixing breed of Japanese chromatic cocoon autosexing race and middle system
Autosexing race is female parent, using the white cocoon kind of the good practicality of middle system's integral economic trait achievement as male parent, and it is new that hybridization is obtained
In be cocoon color autosexing race；To acquisition it is new in be cocoon color autosexing race respectively with general spot, knot yellow green cocoon and plain spot, knot
Two kinds of Breed dirctions of yellow green cocoon respectively reserve seed for planting subculture by seed selection；
(3) with Japanese with white cocoon mixing breed acquisition by Japanese chromatic cocoon autosexing race and Japanese existing production in step (1)
Cocoon color autosexing race for female parent, then using it is Japanese it is existing production with white cocoon kind as male parent, hybridization obtain newly Japanese cocoon color
Autosexing race；Plain spot silkworm, the individual of knot yellow green cocoon or moth area are selected and remain to the new Japanese cocoon color autosexing race of acquisition to reserve seed for planting
Subculture, until homozygosis is plain spot silkworm, full yellow green cocoon；
(4) to being cultivated in step (1), (2), (3) in Japanese cocoon color autosexing race focus on morphological characters base in seed selection early stage
The M8003 line of cause and fixation, the middle and later periods focus on the stable raising of quantitative character；It is sex-limited into yellow green cocoon to complete homozygosis
Kind and the Japanese chromatic cocoon autosexing race of parent, which supported with batch examination, to be compared, Japanese Huang during selection integral economic trait is excellent
Green cocoon autosexing race is parent, carries out hybridization and combines binary first cross combination, carries out laboratory qualification evaluation, as strong
The yellowish green sex-limited cultivated silkworm breed variety of cocoon of property.
2. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that：
Described in step (1) is Japanese chromatic cocoon autosexing race " CLJ-02 " as maternal Japanese chromatic cocoon autosexing race.
3. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that：
The green cocoon kind of the middle system as male parent described in step (1) is the green cocoon kind " CCC-04 " of middle system.
4. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that：
Described in step (1) as being that the good white cocoon kind of integral economic trait achievement is middle system's mixed economy in male parent
The good white cocoon kind " RC-24 " of character achievement.
5. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that：
Described in step (1), (3) is the white cocoon product of Japanese existing production as Japanese existing produce with white cocoon kind of male parent
Plant " Hunan sunshine ".
6. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that：
Described in step (2) as be in male parent the white cocoon kind of the good practicality of integral economic trait achievement be middle assemblage
Close the good white cocoon kind " Feng Xuan " of practicality of economic characters achievement.
7. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that：
Speckle described in step (1) is unified for the unified general spot of selection or unified selectin spot.
8. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that：
Integral economic trait achievement described in step (1), (2) is well worm pupa rate 60%~100%, cocoon weight 1.00g~
2.00g, Cocoon layer ratio 13.00%~28.00%.
9. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that：
Seed selection early stage described in step (4) focus on the M8003 line of morphological characters gene and be fixed as F1~F15 generation emphatically by
According to general spot, tie yellowish green cocoon and plain spot, the yellowish green two kinds of direction seed selections of cocoon of knot, emphatically speckle, cocoon color, the selection of cocoon shape；In described
The stabilization that later stage focuses on quantitative character rises to F15 generations later worm pupa rate, cocoon weight, cocoon shell weight, Cocoon layer ratio economic characters emphatically
Stable raising.
10. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness described in any one of claim 1~9 is in the excellent product of silkworm
Plant the application in cultivation field.
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