CN107318790B - A kind of breeding method of the sex-limited cultivated silkworm breed variety of yellowish green cocoon of robustness and application - Google Patents
A kind of breeding method of the sex-limited cultivated silkworm breed variety of yellowish green cocoon of robustness and application Download PDFInfo
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Abstract
The present invention discloses breeding method and the application of a kind of sex-limited cultivated silkworm breed variety of yellowish green cocoon of robustness.The present invention is successfully realized the cultivation of the sex-limited cultivated silkworm breed variety of yellowish green cocoon for the first time, the kind can predict female ratio in advance in parent silkworm rearing, cocoon mirror pupa recruitment is cut in saving, reduce silkworm egg production cost, and the male and female separation rate of guarantee 100%, the a large amount of recruitments for reducing production of hybrid seeds link simultaneously, greatly improve hybrid seed yield and quality of silkworm eggs, to significantly improve labor efficiency and economic benefit；And can also realize that male and female cocoon separates filature on raw cocoon production, it is utilized respectively, raw silk utility value can be effectively improved, improve the Raw Silk Production and grade of reeling mill, significantly improve economic benefit.The yellow silk of the yellow green cocoon institute reel silk from cocoons of the kind and green silk are natural colorful silk, its moisturizing having the function of, warming, antibacterial, anti-oxidant and masking ultraviolet light etc. are better than silkworm white silk, has the characteristics that natural, green, low-carbon, its exploitation meets Current resource saving, environmental-friendly idea of development.
Description
Technical field
The invention belongs to the excellent characteristic Silkworm varieties of silkworm to cultivate field, and in particular to a kind of sex-limited silkworm of yellowish green cocoon of robustness
The breeding method of kind and application.
Background technique
Natural colorful silk has the function of that moisturizing, warming, antibacterial, anti-oxidant and masking ultraviolet light etc. are white better than silkworm simultaneously
Color silk, natural colorful cocoon resource are the primary kinds of nature, have the characteristics that " natural, green, low-carbon ", develop and meet to it
The currently idea of development of " resource-effective, environmental-friendly ", there is very wide development prospect.The sex-limited germplasm of cocoon color is to use
The female pupa of 60Cor radiation exposure silkworm makes on the yellow blood group translocation to W chromosome on its autosome, obtains sex-limited cocoon mutation
System, the female silkworm knot Yellow Cocoon being bred as based on this, male silkworm knot white cocoon.The research of cocoon color autosexing race is in order in parent silkworm rearing
In predict female ratio in advance, cocoon mirror pupa recruitment is cut in saving, reduces silkworm egg production cost；And male and female can also be realized on raw cocoon production
Cocoon is utilized respectively, and improves raw silk utility value, carries out the research of cocoon color autosexing race, to significantly improving silkworm raiser and silk enterprise
Economic benefit has important practical significance.Currently, domestic sex-limited (yellowish-white cocoon color the is sex-limited) silkworm of cocoon color that not yet can be practical
The incubation of kind, the sex-limited Silkworm varieties of yellow, green cocoon has not been reported, and therefore, carries out the sex-limited silkworm breeding material of yellow, the green cocoon of robustness
Material innovation, superior to the diversification, the silk product that accelerate Guangdong production Silkworm varieties, adapting to silk product structural adjustment has
Very big effect.
Summary of the invention
In order to overcome the disadvantages and deficiencies of the prior art, the purpose of the present invention is to provide a kind of yellowish green cocoon of robustness is sex-limited
The breeding method of cultivated silkworm breed variety.
Another object of the present invention is to provide the applications of above-mentioned breeding method.
The purpose of the invention is achieved by the following technical solution:
A kind of breeding method of the sex-limited cultivated silkworm breed variety of yellowish green cocoon of robustness, includes the following steps:
(1) be female parent with Japanese chromatic cocoon autosexing race, respectively with middle system green cocoon kind, middle system's integral economic trait at
The good white cocoon kind of achievement and Japanese existing produce with white cocoon kind are male parent, and it is Cocoon color that hybridization, which obtains in new, respectively
Autosexing race and new Japanese Cocoon color autosexing race；To acquisition it is new in be Cocoon color autosexing race and new Japanese
The F1 generation of Cocoon color autosexing race is respectively adopted the mixing of 0.2~1.0g ant amount and educates, and F2 generation starts using one batch rearing, selection tool
Dapple unification ties yellow cocoon and the good individual or moth area of green cocoon, integral economic trait is reserved seed for planting breeding, by mostly for directive breeding,
It cultivates and has the sex-limited feature of yellow green, i.e., Huang cocoon is female, and green cocoon is hero, and integral economic trait reaches or approaches commercial variety
New cocoon color autosexing race, to realize the cultivation purpose of the sex-limited commercial variety of yellow green；
(2) in step (1) by the green cocoon mixing breed of Japanese chromatic cocoon autosexing race and middle system obtain in be silkworm
Cocoon color autosexing race is female parent, and using the middle white cocoon kind of the good practicability of system's integral economic trait achievement as male parent, hybridization is obtained
It is new in be cocoon color autosexing race；To acquisition it is new in be cocoon color autosexing race respectively with general spot, knot yellow green cocoon and element
Breeding is reserved seed for planting subculture respectively for spot, knot two kinds of Breed dirctions of yellow green cocoon；
(3) to be obtained in step (1) by Japanese chromatic cocoon autosexing race and the Japanese existing white cocoon mixing breed of production
Japanese Cocoon color autosexing race be female parent, then using it is Japanese it is existing production with white cocoon kind as male parent, hybridization obtain newly it is Japanese
Cocoon color autosexing race；Plain spot silkworm, the individual for tying yellow green cocoon or moth area are selected and remain to the new Japanese cocoon color autosexing race of acquisition
It reserves seed for planting subculture, until homozygous for plain spot silkworm, full yellow green cocoon；
(4) early period form is focused in breeding to Japanese cocoon color autosexing race in cultivating in step (1), (2), (3)
The M8003 line of shape gene and fixation, middle and later periods focus on stablizing for quantitative character and improve；It is limited at yellow green cocoon completely homozygous
Property the Japanese chromatic cocoon autosexing race of kind and parent carry out trying to support with batch comparing, select excellent Sino-Japan of integral economic trait
Be yellowish green cocoon autosexing race be parent, carry out hybridization combine binary first cross combination, carry out laboratory qualification evaluation, as
The sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness.
Japanese chromatic cocoon autosexing race described in step (1) as female parent is preferably Japanese chromatic cocoon autosexing race
"CLJ-02"；
Preferably middle as middle system's green cocoon kind of male parent described in step (1) is green cocoon kind " CCC-04 "；
Described in step (1) as be in male parent the good white cocoon kind of integral economic trait achievement preferably in system
The good white cocoon kind " RC-24 " of integral economic trait achievement；
Japanese existing produce with white cocoon kind described in step (1), (3) as male parent is preferably Japanese existing production
With white cocoon kind " Hunan sunshine "；
As being that the white cocoon kind of the good practicability of integral economic trait achievement is preferred in male parent described in step (2)
For the white cocoon kind " Feng Xuan " of the middle good practicability of system's integral economic trait achievement；
The unified preferably unified general spot of selection of speckle described in step (1) or unified selectin spot；
Integral economic trait achievement described in step (1), (2) is well preferably worm pupa rate 60%~100%, cocoon weight
1.00g~2.00g, Cocoon layer ratio 13.00%~28.00%；
The M8003 line and fixed preferably F1~F15 of morphological characters gene are focused in breeding described in step (4) early period
In generation, is emphatically according to general spot, the yellowish green cocoon of knot and plain spot, the yellowish green two kinds of direction breedings of cocoon of knot, the emphatically selection of speckle, cocoon color, cocoon shape；
The middle and later periods focuses on stablizing for quantitative character and improves preferably F15 generation later worm pupa rate, cocoon weight, cocoon shell weight, cocoon emphatically
Stablizing for the economic characters such as layer rate improves；Preferably through mostly for breeding to realize that the vitality for making cultivation kind reaches or approaches reality
With the target of varietal level；
The breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of the robustness, preferably comprises following steps:
(1) be female parent with Japanese chromatic cocoon autosexing race " CLJ-02 ", respectively with middle system green cocoon kind " CCC-04 ", in
It is the good white cocoon kind " RC-24 " of integral economic trait achievement, Japanese existing production is male parent with white cocoon kind " Hunan sunshine ", point
Za Jiao not obtain is Cocoon color autosexing race " CLJ-02 × CCC-04 " (referred to as " CLC-03 "), " CLJ-02 × RC- in new
24 " (referred to as " CLC-04 ") and Japanese Cocoon color autosexing race " CLJ-02 × Hunan sunshine " (referred to as " CLJ-06 "), to acquisition
The F1 generation of " CLC-03 ", " CLC-04 " and " CLJ-06 " are respectively adopted the mixing of 0.5g ant amount and educate raising, and F2 generation starts using single moth
Educate raising.
It (2) is that the F1 generation of Cocoon color autosexing race " CLC-04 " is yellowish green for general spot, knot in hybridization acquisition in step (1)
Bai Jian selects and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserves seed for planting subculture, and F2 is yellowish green white for plain spot silkworm, knot is isolated in general spot
Cocoon selects and remain plain spot silkworm, yellow green cocoon subculture, until F7 generation is homozygous for plain spot silkworm, full yellow green cocoon；
It (3) is that the F1 generation of Cocoon color autosexing race " CLC-03 " is yellowish green for general spot, knot in hybridization acquisition in step (1)
In Bai Jian, continuous 6 generation, select and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserve seed for planting subculture, until F7 generation is homozygous is general spot silkworm, complete yellowish green
Color cocoon；
(4) with hybridization in step (1) obtain in be Cocoon color autosexing race " CLC-03 " F1 on behalf of female parent, in
It is the white cocoon kind " Feng Xuan " of the good practicability of integral economic trait achievement is male parent, it is the sex-limited product of cocoon color that hybridization, which obtains in new,
Kind " CLC-03 × Feng Xuan ", " CLC-03 × Feng Xuan " F1 generation are general spot, the yellowish green white cocoon of knot, and F2 is yellowish green white on behalf of general spot, plain spot, knot
Cocoon selects and remain general spot, ties the individual of yellow green cocoon or moth area reserves seed for planting subculture, is named as " CLC-05 ", selects and remain plain spot, knot Yellow Cocoon
Individual or moth area reserve seed for planting subculture, be named as " CLC-06 ".
(5) " CLC-05 " one obtained in step (4) reserves seed for planting until selecting and remain general spot, the individual of knot yellow green cocoon or moth area
Subculture until it is general spot silkworm that F5 generation is homozygous, but ties yellowish green white cocoon, until it is general spot silkworm, full yellow green cocoon that F10 generation is homozygous；It obtains
" CLC-06 " then select and remain always plain spot, tie the individual of yellow green cocoon or moth area reserves seed for planting subculture, until F5 band is homozygous for plain spot silkworm, knot
Yellowish green white cocoon, but do not go out homozygosis also for cocoon color to F22, still tie yellow, yellow, green, the white cocoon of powder.
(6) it is yellowish green for general spot, knot to hybridize the F1 generation of the Japanese Cocoon color autosexing race " CLJ-06 " obtained in step (1)
Bai Jian selects and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserves seed for planting subculture, and F2 is yellowish green white for plain spot silkworm, knot is isolated in general spot
Cocoon selects and remain plain spot silkworm, yellow green cocoon subculture, until F7 generation is homozygous for plain spot silkworm, full yellow green cocoon；
It (7) is female parent to obtain Japanese silkworm autosexing race " CLJ-06 " F3 generation (plain spot silkworm) in step (6), then with Japanese
Existing production is male parent with white cocoon kind " Hunan sunshine ", and hybridization obtains the (letter of new Japanese cocoon color autosexing race " CLJ-06 × Hunan sunshine "
Claim " CLJ-07 "), select and remain plain spot silkworm, tie the individual of yellow green cocoon or moth area reserves seed for planting subculture, until F13 generation it is homozygous for plain spot silkworm,
Full yellow green cocoon.
(8) to be in being cultivated in step (2), (3), (5), (6), (7) Cocoon color autosexing race " CLC-03 ",
" CLC-04 ", " CLC-05 ", " CLC-06 " and Japanese Cocoon color autosexing race " CLJ-06 ", " CLJ-07 " this 6 kinds exist
M8003 line and the fixation of morphological characters gene are focused in breeding early period, and the middle and later periods focuses on stablizing for quantitative character and improves.To 5
It is completely homozygous to carry out trying to support with batch comparing at the sex-limited kind of yellow green cocoon and parent " CLJ-02 ", select integral economic trait
Excellent middle system " CLC-04 ", Japanese " CLJ-06 " carry out hybridization and combine binary first cross combination, carry out laboratory qualification
Evaluation obtains the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness " CLC-04 × CLJ-06 ".
In step (1), the main feature characteristic of the parent " CLJ-02 " are as follows: Japanese bivoltine four-moulter kind,
Larva body spot is general spot；It hastens the hatching of silkworms by 10 days, larval phase passes through 21~22, passes through 14~15 in bruising；Worm pupa rate 65%~
100%；Cocoon shape is with a tight waist, and yellowish green (a small number of white cocoons) cocoon is sex-limited, yellow cocoon be it is female, green, white cocoon is hero；1.4~1.8g of cocoon weight, cocoon layer
Rate 17%~20%；
In step (1), the main feature characteristic of the parent " CCC-04 " are as follows: in be bivoltine four-moulter kind,
Larva body spot is general spot；It hastens the hatching of silkworms by 10 days, larval phase passes through 19~20, passes through 12~13 in bruising；Worm pupa rate 70%~
100%；Cocoon shape is oval, cocoon green (having only a few white cocoon), 1.1~1.5g of cocoon weight, Cocoon layer ratio 14%~17%；
In step (1), the main feature characteristic of the parent " RC-24 " are as follows: in be bivoltine four-moulter kind；Children
Polypide spot is plain spot；It hastens the hatching of silkworms by 10 days, larval phase passes through 21~23, passes through 14~16 in bruising；Worm pupa rate 70%~
100%；Cocoon shape is oval, and cocoon color is pure white；1.4~1.8g of cocoon weight, Cocoon layer ratio 22%~26%；
It is Cocoon color autosexing race in incubation with " CLJ-02 " and " CCC-04 " for parent in step (1)
The main feature characteristic of " CLC-03 " are as follows: in be bivoltine four-moulter kind；Larva body spot is general spot；It hastens the hatching of silkworms by 10 days, children
The worm phase passes through 19~21, passes through 14~16 in bruising；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow
Cocoon is that female, green cocoon is male；1.4~1.8g of cocoon weight, Cocoon layer ratio 16%~20%；
It is Cocoon color autosexing race " CLC- in incubation with " CLJ-02 " and " RC-24 " for parent in step (1)
04 " main feature characteristic are as follows: in be bivoltine four-moulter kind；Larva body spot is plain spot；It hastens the hatching of silkworms by 10 days, larval phase passes through
19~21 are spent, is passed through 13~15 in bruising；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow cocoon is
Female, green cocoon is hero；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；
In step (1), with " CLJ-02 " and " Hunan sunshine " for parent, the Japanese Cocoon color autosexing race " CLJ- of incubation
06 " main feature characteristic are as follows: Japanese bivoltine four-moulter kind；Larva body spot is plain spot；It hastens the hatching of silkworms by 10 days, larval phase passes through
20~22 are spent, is passed through 13~15 in bruising；Worm pupa rate 80%~100%；Cocoon shape is with a tight waist, and yellowish green cocoon is sex-limited, i.e., yellow cocoon is
Female, green cocoon is hero；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；
It is cocoon color autosexing race " CLC-05 " in incubation with " CLC-03 " and " Feng Xuan " for parent in step (4)
Main feature characteristic are as follows: in be bivoltine four-moulter kind；Larva body spot is general spot；It hastens the hatching of silkworms by 10 days, larval phase passes through 20
~22, pass through 13~15 in bruising；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow cocoon is female, green cocoon
For hero；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；It is the main spy of cocoon color autosexing race " CLC-06 " in being bred as
Intrinsic characteristics are as follows: in be bivoltine four-moulter kind；Larva body spot is plain spot；It hastens the hatching of silkworms by 10 days, larval phase, passes through 20~22,
Pass through 13~15 in bruising；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green white cocoon is sex-limited, i.e., Huang cocoon is female, and green, white cocoon is
It is male；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；
In step (7), with " CLJ-06 " and " Hunan sunshine " for parent, the Japanese Cocoon color autosexing race " CLJ- of incubation
07 " main feature characteristic are as follows: Japanese bivoltine four-moulter kind；Larva body spot is plain spot；It hastens the hatching of silkworms by 10 days, larval phase passes through
20~22 are spent, is passed through 14~16 in bruising；Worm pupa rate 75%~100%；Cocoon shape is with a tight waist, and yellowish green cocoon is sex-limited, i.e., yellow cocoon is
Female, green cocoon is hero；1.4~1.9g of cocoon weight, Cocoon layer ratio 18%~22%.
In step (8), the main feature of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of the robustness " CLC-04 × CLJ-06 "
Characteristic are as follows: binary first cross kind, larva body spot are plain spot；Silkworm hatching, molting, homogeneous of being placed on small straw bundles to spin cocoons, cocoon color is bright-coloured, yellowish green cocoon
Sex-limited, i.e., Yellow Cocoon is female, and green cocoon is hero；Silkworm body is strong, and easily raising, easily breeding, diapause performance are stablized, integral economic trait
It is excellent；
The excellent preferably worm pupa rate 80%~100% of the integral economic trait, cocoon weight 1.30g~2.2g, cocoon layer
Rate 16.00%~24.00%.
The breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of the robustness answering in silkworm excellent variety cultivation field
With.
The present invention compared with the existing technology, have following advantages and effects
(1) present invention is successfully realized the cultivation of the sex-limited cultivated silkworm breed variety of yellowish green cocoon for the first time, which can utilize cocoon in pupa time
Color difference female is separated, developmental duration, robustness, cocoon quality achievement it is different degrees of be better than yellowish green (white) the sex-limited mesh of color cocoon
Gene parent CLJ-02 is marked, and other Major Economics reach or approach the level of commercial variety.
(2) kind can predict female ratio in advance in parent silkworm rearing, and saving cuts cocoon mirror pupa recruitment, reduces silkworm egg and be produced into
This, and the male and female separation rate of guarantee 100%, while a large amount of recruitments of production of hybrid seeds link are reduced, greatly improve hybrid seed yield and silkworm egg
Quality, to significantly improve the labor efficiency and economic benefit of seed station；And male and female cocoon can also be realized on raw cocoon production and separates reel silk from cocoons
Silk, is utilized respectively, can effectively improve raw silk utility value, improve the Raw Silk Production and grade of reeling mill, significantly improve reeling mill
Economic benefit.
(3) the yellow silk of the yellow green cocoon institute reel silk from cocoons of the kind and green silk are natural colorful silk, the moisturizing having,
The functions such as warming, antibacterial, anti-oxidant and masking ultraviolet light are better than silkworm white silk, have the spy of " natural, green, low-carbon "
Point, exploitation meet the idea of development of current " resource-effective, environmental-friendly ".
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
The middle white cocoon kind " Feng Xuan " of the good practicability of system's integral economic trait achievement is by Guangdong Province's sericulture Technique Popularizing
(Guo Dingguo, Lin Jianrong, the yellow concubine of an emperor wait incubation [J] silkworm of new silkworm variety Guangdong maple No. three (five choosings × 2832) for heart offer
Industry science, 2007,33 (3): 466~469.)
Japanese existing production with white cocoon kind " Hunan sunshine " by Guangdong Silkworm Spread Center's offer (look after one's family, Shen Chang
Flat, Yao Fuguang waits 932 lotuses of silkworm summer and autumn new varieties × Hunan sunshine 7532 (Guangdong and Guangxi Provinces two) Guangxi incubation [J] silkworm
Industry, 1995, (1): 44~48.)
" CLJ-02 ", " CCC-04 ", the purchase of " RC-24 " these three cultivated silkworm breed varieties are in Guangdong Silkworm Spread Center.
Embodiment 1
(1) 2005 year autumn started respectively with Japanese chromatic cocoon autosexing race " CLJ-02 " for female parent, with the green cocoon product of middle system
Kind " CCC-04 ", the good white cocoon kind " RC-24 " of middle system's integral economic trait achievement, the white cocoon kind of Japanese existing production
" Hunan sunshine " is male parent, and it is Cocoon color autosexing race " CLJ-02 × CCC-04 " (referred to as " CLC- in new that hybridization, which obtains, respectively
03 "), " CLJ-02 × RC-24 " (referred to as " CLC-04 ") and Japanese Cocoon color autosexing race " CLJ-02 × Hunan sunshine " (abbreviation
" CLJ-06 "), the mixing of 0.5g ant amount is respectively adopted to the F1 generation of " CLC-03 " of acquisition, " CLC-04 " and " CLJ-06 " and educates feeding
It supports, F2 generation starts to raise using one batch rearing, wherein hybridizing in acquisition is Cocoon color autosexing race " CLC-04 ", and F1 generation is
General spot, the yellowish green white cocoon of knot select and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserve seed for planting subculture, and F2 is for isolating plain spot in general spot
Silkworm, the yellowish green white cocoon of knot, select and remain plain spot silkworm, yellow green cocoon subculture, until F7 generation is homozygous for plain spot silkworm, full yellow green cocoon；"CLC-03"
F1 generation be general spot, the yellowish green white cocoon of knot, in continuous 6 generation, selects and remain general spot silkworm, the individual of yellow green cocoon or moth area and reserves seed for planting subculture, until F7 generation
Homozygosis is general spot silkworm, full yellow green cocoon；The F1 generation of Japanese Cocoon color autosexing race " CLJ-06 " is general spot, ties yellowish green white cocoon,
It selects and remain general spot silkworm, the individual of yellow green cocoon or moth area to reserve seed for planting subculture, F2 is for isolating plain spot silkworm, the yellowish green white cocoon of knot, choosing in general spot
Plain spot silkworm, yellow green cocoon subculture are stayed, until F7 generation is homozygous for plain spot silkworm, full yellow green cocoon.Wherein, the main feature of " CLJ-02 " is special
Property are as follows: Japanese bivoltine four-moulter kind, larva body spot are general spot；It hastens the hatching of silkworms by 10 days, larval phase passes through 21~22, in bruising
By 14~15 days；Worm pupa rate 65%~100%；Cocoon shape is with a tight waist, and yellowish green (a small number of white cocoons) cocoon is sex-limited, and yellow cocoon is female, green, Bai Jian
For hero；1.4~1.8g of cocoon weight, Cocoon layer ratio 17%~20%；The main feature characteristic of " CCC-04 " are as follows: in be bivoltine four
Hibernating worm kind, larva body spot are general spot；It hastens the hatching of silkworms by 10 days, larval phase passes through 19~20, passes through 12~13 in bruising；Worm
Pupa rate 70%~100%；Cocoon shape is oval, cocoon green (having only a few white cocoon), 1.1~1.5g of cocoon weight, and Cocoon layer ratio 14%~
17%；The main feature characteristic of " RC-24 " are as follows: in be bivoltine four-moulter kind；Larva body spot is plain spot；It hastens the hatching of silkworms by 10
Day, larval phase, passes through 21~23, passes through 14~16 in bruising；Worm pupa rate 70%~100%；Cocoon shape is oval, and cocoon color is pure white；Entirely
Cocoon 1.4~1.8g of amount, Cocoon layer ratio 22%~26%.
(2) to be the F1 generation of Cocoon color autosexing race " CLC-03 " in step (1) hybridization acquisition for female parent, middle assemblage
The conjunction white cocoon kind " Feng Xuan " of the good practicability of economic characters achievement is male parent, and it is cocoon color autosexing race that hybridization, which obtains in new,
" CLC-03 × Feng Xuan ", the F1 generation of " CLC-03 × Feng Xuan " are general spot, the yellowish green white cocoon of knot, and F2 is yellowish green white on behalf of general spot, plain spot, knot
Cocoon selects and remain general spot, ties the individual of yellow green cocoon or moth area reserves seed for planting subculture, is named as " CLC-05 ", selects and remain plain spot, knot Yellow Cocoon
Individual or moth area reserve seed for planting subculture, be named as " CLC-06 ".Wherein, " CLC-05 " one is until select and remain general spot, knot yellow green cocoon
Individual or moth area reserve seed for planting subculture, until it is general spot silkworm that F5 generation is homozygous, but yellowish green white cocoon is tied, until F10 generation is homozygous is general spot silkworm, complete
Yellow green cocoon；" CLC-06 " obtained then select and remain always plain spot, tie the individual of yellow green cocoon or moth area reserves seed for planting subculture, until F5 band
Homozygosis is plain spot silkworm, ties yellowish green white cocoon, but do not go out homozygosis also for cocoon color to F22, still ties yellow, yellow, green, the white cocoon of powder.
It (3) is female parent with the F3 generation (plain spot silkworm) of the Japanese Cocoon color autosexing race " CLJ-06 " obtained in step (1),
Again with it is Japanese it is existing production with white cocoon kind " Hunan sunshine " be male parent, hybridization obtain newly Japanese Cocoon color autosexing race " CLJ-
06 × Hunan sunshine " (referred to as " CLJ-07 ") selects and remain plain spot silkworm, ties the individual of yellow green cocoon or moth area reserves seed for planting subculture, until F13 is for pure
It is combined into plain spot silkworm, full yellow green cocoon.
(4) to be in being cultivated in step (1), (2), (3) Cocoon color autosexing race " CLC-03 ", " CLC-04 ",
" CLC-05 ", " CLC-06 " and Japanese Cocoon color autosexing race " CLJ-06 ", " CLJ-07 " this 6 kinds are in breeding early period
The M8003 line of weight morphological characters gene and fixation, middle and later periods focus on stablizing for quantitative character and improve.By breeding in more than 10 years,
In the Japanese kind being respectively bred as breeding more than 20 generations, currently, form and Major Economic achievement are successively stablized.Wherein,
The main feature characteristic of " CLC-03 " are as follows: in be bivoltine four-moulter kind；Larva body spot is general spot；It hastens the hatching of silkworms by 10 days, children
The worm phase passes through 19~21, passes through 14~16 in bruising；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., yellow
Cocoon is that female, green cocoon is male；1.4~1.8g of cocoon weight, Cocoon layer ratio 16%~20%；The main feature characteristic of " CLC-04 " are as follows: in
It is bivoltine four-moulter kind；Larva body spot is plain spot；It hastens the hatching of silkworms by 10 days, larval phase passes through 19~21, and 13 are passed through in bruising
~15 days；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green cocoon is sex-limited, i.e., it is male that yellow cocoon, which is female, green cocoon,；Cocoon weight 1.5~
1.9g, Cocoon layer ratio 19%~22%；The main feature characteristic of " CLC-05 " are as follows: in be bivoltine four-moulter kind；Larva body spot
For general spot；It hastens the hatching of silkworms by 10 days, larval phase passes through 20~22, passes through 13~15 in bruising；Worm pupa rate 80%~100%；Cocoon
Shape is oval, and yellowish green cocoon is sex-limited, i.e., it is male that yellow cocoon, which is female, green cocoon,；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；"CLC-
06 " main feature characteristic are as follows: in be bivoltine four-moulter kind；Larva body spot is plain spot；It hastens the hatching of silkworms by 10 days, larval phase passes through
20~22 are spent, is passed through 13~15 in bruising；Worm pupa rate 80%~100%；Cocoon shape is oval, and yellowish green white cocoon is sex-limited, i.e., yellow cocoon is
Female, green, white cocoon is hero；1.5~1.9g of cocoon weight, Cocoon layer ratio 19%~22%；The main feature characteristic of " CLJ-06 " are as follows: Japanese
Bivoltine four-moulter kind；Larva body spot is plain spot；Hasten the hatching of silkworms by 10 days, larval phase, passes through 20~22, in bruising by 13~
15 days；Worm pupa rate 80%~100%；Cocoon shape is with a tight waist, and yellowish green cocoon is sex-limited, i.e., it is male that yellow cocoon, which is female, green cocoon,；Cocoon weight 1.5~
1.9g, Cocoon layer ratio 19%~22%.The main feature characteristic of " CLJ-07 " are as follows: Japanese bivoltine four-moulter kind；Larva body spot
For plain spot；It hastens the hatching of silkworms by 10 days, larval phase passes through 20~22, passes through 14~16 in bruising；Worm pupa rate 75%~100%；Cocoon
Pencil of forms waist, yellowish green cocoon is sex-limited, i.e., it is male that yellow cocoon, which is female, green cocoon,；1.4~1.9g of cocoon weight, Cocoon layer ratio 18%~22%.
(5) 5 completely homozygous support at the sex-limited kind of yellow green cocoon and parent " CLJ-02 " progress with batch examination are compared
It is different degrees of compared with the developmental duration of, the results showed that 5 yellow green cocoon autosexing races, robustness, cocoon quality achievement better than yellowish green
The sex-limited target gene parent " CLJ-02 " of (white) color cocoon.Wherein, most outstanding is middle system " CLC-04 ", Japanese " CLJ-06 ", 5 ages
Through too short 1 day, full age, full period was through too short 3 days through too short 3 days；22.45 percentage points and 10.88 hundred are respectively increased in robustness
Branch；Cocoon weight increases, and Cocoon layer ratio improves 1.67 percentage points and 0.79 percentage point (see Table 1 for details).
(6) basis newly creates the property stability and integral economic trait performance of each sex-limited breeding material of yellow green cocoon,
It selects with the excellent middle system " CLC-04 " of integral economic trait and Japanese " CLJ-06 " as parent, it is miscellaneous that hybridization combines a binary generation
Combination is handed over to carry out laboratory qualification evaluation, No. two compare with existing production kind Guangdong and Guangxi Provinces, Guangdong.The result shows that: yellowish green cocoon cocoon color
The main feature characteristic of sex-limited first cross combination " CLC-04 × CLJ-06 " (reciprocal cross) is: hatching molting homogeneous, food mulberry is fast,
Silkworm body is elongated, full element spot silkworm；It is aging to gush together, bury that cage is fast, and cocoon shape is neat and well spaced, cocoon color is bright-coloured, Yellow Cocoon be it is female, green cocoon is hero；Entirely
Age passes through 20 days or so, and 5 ages passed through 5 days half；Worm pupa rate 93.32%, than No. two low 1.12 percentage points of Guangdong and Guangxi Provinces；Ten thousand silkworms receive cocoon
13.79% and 15.80% is respectively increased in amount, ten thousand silkworm cocoon shell weights；Can male and female cocoon distinguish filature, with the male and female silk union ratio of Guangdong and Guangxi Provinces two
Compared with male one silk of cocoon improves 17.00% up to 1246 meters, and 2.078 dtex of the size of cocoon filament is thin by 15.74%, and light rolls over 243Kg drop
Low 7.00%, cleanliness 93.50 divides raising 3.89%, and dry hair cocoon silk production rate 38.65% improves 6.39% (see Table 2 for details~table 3).
The newly 6 Yellow Cocoon autosexing race subculture major traits achievement investigation of wound of table 1
The average identification achievement (making for 2014 2,6) in 2 batch laboratory of table 2 " CLC-04 × CLJ-06 " (reciprocal cross)
The silk quality in 2 batch laboratory of table 3 " CLC-04 × CLJ-06 " (reciprocal cross) averagely identifies achievement (making within 2014 2,6)
Only 2 make the detection of sample presentation cocoon filature, cocoon rate of getting on the bus in table, silk length, reelability percentage, length of non broken filament, Cocoon Silk Weight, fiber number,
Light folding, cleaning, dry hair cocoon silk production rate are identified by sericulture there product quality supervision and inspection test center, the Ministry of Agriculture (Zhenjiang).CLC-
04 × CLJ-06 points female (Huang) male (green) cocoons examine silk quality respectively, and Guangdong and Guangxi Provinces two (CK) are that the mixing of male and female cocoon is examined.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of breeding method of the sex-limited cultivated silkworm breed variety of yellowish green cocoon of robustness, it is characterised in that include the following steps:
It (1) is female parent with Japanese chromatic cocoon autosexing race, it is good with the green cocoon kind of middle system, middle system's integral economic trait achievement respectively
Good white cocoon kind and Japanese existing produce with white cocoon kind are male parent, and it is that Cocoon color is sex-limited that hybridization, which obtains in new, respectively
Kind and new Japanese Cocoon color autosexing race；To acquisition it is new in be Cocoon color autosexing race and new Japanese silkworm
The F1 generation of cocoon color autosexing race is respectively adopted the mixing of 0.2~1.0g ant amount and educates, and F2 generation starts using one batch rearing, and selection has speckle
It is unified, tie yellow cocoon and the good individual or moth area of green cocoon, integral economic trait is reserved seed for planting breeding, by mostly for directive breeding, cultivating
Have the sex-limited feature of yellow green, i.e., yellow cocoon be it is female, green cocoon is hero, and integral economic trait reaches or approaches the new of commercial variety
Cocoon color autosexing race, to realize the cultivation purpose of the sex-limited commercial variety of yellow green；
(2) in step (1) by the green cocoon mixing breed of Japanese chromatic cocoon autosexing race and middle system obtain in be Cocoon color
Autosexing race is female parent, using the middle white cocoon kind of the good practicability of system's integral economic trait achievement as male parent, hybridizes the new of acquisition
In be cocoon color autosexing race；To acquisition it is new in be cocoon color autosexing race respectively with general spot, knot yellow green cocoon and plain spot, knot
Two kinds of Breed dirctions of yellow green cocoon respectively reserve seed for planting subculture by breeding；
(3) Japanese to be obtained in step (1) by Japanese chromatic cocoon autosexing race and the white cocoon mixing breed of Japanese existing production
Cocoon color autosexing race be female parent, then using it is Japanese it is existing production with white cocoon kind as male parent, hybridization obtain newly Japanese cocoon color
Autosexing race；Plain spot silkworm, the individual of knot yellow green cocoon or moth area is selected and remain to the new Japanese cocoon color autosexing race of acquisition to reserve seed for planting
Subculture, until homozygous for plain spot silkworm, full yellow green cocoon；
(4) early period morphological characters base is focused in breeding to Japanese cocoon color autosexing race in cultivating in step (1), (2), (3)
The M8003 line of cause and fixation, middle and later periods focus on stablizing for quantitative character and improve；To completely homozygous sex-limited at yellow green cocoon
Kind and the Japanese chromatic cocoon autosexing race of parent carry out trying to support with batch comparing, select integral economic trait it is excellent in Japanese Huang
Green cocoon autosexing race is parent, carries out hybridization and combines binary first cross combination, carries out laboratory qualification evaluation, as strong
The yellowish green sex-limited cultivated silkworm breed variety of cocoon of property.
2. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that:
Japanese chromatic cocoon autosexing race described in step (1) as female parent is Japanese chromatic cocoon autosexing race " CLJ-02 ".
3. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that:
It as middle system's green cocoon kind of male parent is middle system green cocoon kind " CCC-04 " described in step (1).
4. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that:
Described in step (1) as be in male parent the good white cocoon kind of integral economic trait achievement be middle system's mixed economy
The good white cocoon kind " RC-24 " of character achievement.
5. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that:
The white cocoon kind of Japanese existing production described in step (1), (3) as male parent is the white cocoon product of Japanese existing production
Kind " Hunan sunshine ".
6. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that:
Described in step (2) as be in male parent the white cocoon kind of the good practicability of integral economic trait achievement be middle assemblage
Close the white cocoon kind " Feng Xuan " of the good practicability of economic characters achievement.
7. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that:
Speckle described in step (1) is unified for the general spot of unified selection or unified selectin spot.
8. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that:
Integral economic trait achievement described in step (1), (2) is well worm pupa rate 60%~100%, cocoon weight 1.00g~
2.00g, Cocoon layer ratio 13.00%~28.00%.
9. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to claim 1, it is characterised in that:
Breeding described in step (4) focus on early period morphological characters gene M8003 line and be fixed as F1~F15 generation emphatically by
According to general spot, the yellowish green cocoon of knot and plain spot, the yellowish green two kinds of direction breedings of cocoon of knot, the emphatically selection of speckle, cocoon color, cocoon shape；In described
The stabilization that later period focuses on quantitative character rises to F15 generation later worm pupa rate, cocoon weight, cocoon shell weight, Cocoon layer ratio economic characters emphatically
Stablize improve.
10. the breeding method of the sex-limited cultivated silkworm breed variety of the yellowish green cocoon of robustness according to any one of claims 1 to 9 is in the excellent product of silkworm
Application in kind cultivation field.
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