CN107821342B - Breeding method of silkworm with multiple silks and capable of resisting blood type pyosis - Google Patents
Breeding method of silkworm with multiple silks and capable of resisting blood type pyosis Download PDFInfo
- Publication number
- CN107821342B CN107821342B CN201711238086.1A CN201711238086A CN107821342B CN 107821342 B CN107821342 B CN 107821342B CN 201711238086 A CN201711238086 A CN 201711238086A CN 107821342 B CN107821342 B CN 107821342B
- Authority
- CN
- China
- Prior art keywords
- silkworm
- variety
- breeding
- cocoon
- resistance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 184
- 238000009395 breeding Methods 0.000 title claims abstract description 77
- 239000008280 blood Substances 0.000 title claims abstract description 37
- 210000004369 blood Anatomy 0.000 title claims abstract description 34
- 230000001488 breeding effect Effects 0.000 claims abstract description 57
- 238000000034 method Methods 0.000 claims abstract description 28
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 22
- 235000003642 hunger Nutrition 0.000 claims abstract description 19
- 230000037351 starvation Effects 0.000 claims abstract description 17
- 238000009396 hybridization Methods 0.000 claims abstract description 16
- 230000000384 rearing effect Effects 0.000 claims abstract description 15
- 206010025482 malaise Diseases 0.000 claims abstract description 14
- 208000035240 Disease Resistance Diseases 0.000 claims abstract description 10
- 238000012360 testing method Methods 0.000 claims description 20
- 241000894007 species Species 0.000 claims description 13
- 240000000249 Morus alba Species 0.000 claims description 9
- 235000008708 Morus alba Nutrition 0.000 claims description 9
- 240000005020 Acaciella glauca Species 0.000 claims description 4
- 235000008582 Pinus sylvestris Nutrition 0.000 claims description 4
- 239000001839 pinus sylvestris Substances 0.000 claims description 4
- 235000013601 eggs Nutrition 0.000 description 16
- 238000011161 development Methods 0.000 description 12
- 230000018109 developmental process Effects 0.000 description 12
- 238000004519 manufacturing process Methods 0.000 description 11
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 10
- 235000011613 Pinus brutia Nutrition 0.000 description 10
- 241000018646 Pinus brutia Species 0.000 description 10
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical group [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 10
- 230000017448 oviposition Effects 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 230000013011 mating Effects 0.000 description 7
- 241000382353 Pupa Species 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 230000036541 health Effects 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 235000005205 Pinus Nutrition 0.000 description 3
- 241000218602 Pinus <genus> Species 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 210000004209 hair Anatomy 0.000 description 3
- 230000012447 hatching Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 241000256602 Isoptera Species 0.000 description 2
- 244000248021 Vitex negundo Species 0.000 description 2
- 235000010363 Vitex negundo Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- RDFLLVCQYHQOBU-GPGGJFNDSA-O Cyanin Natural products O([C@H]1[C@H](O)[C@H](O)[C@H](O)[C@H](CO)O1)c1c(-c2cc(O)c(O)cc2)[o+]c2c(c(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O3)cc(O)c2)c1 RDFLLVCQYHQOBU-GPGGJFNDSA-O 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 230000003460 anti-nuclear Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- RDFLLVCQYHQOBU-ZOTFFYTFSA-O cyanin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=[O+]C1=CC(O)=C2)C=3C=C(O)C(O)=CC=3)=CC1=C2O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 RDFLLVCQYHQOBU-ZOTFFYTFSA-O 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009432 framing Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000010396 two-hybrid screening Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a breeding method of silkworm with multi-silk content and resistance to blood type running sickness, belonging to the field of silkworm breedingIn the technical field of silkworm breeding, the method comprises the following steps: respectively hybridizing a basic material RB carrying a blood-type pyosis resistance gene with a middle-line pure seed 881 and a daily-line pure seed 882 to obtain F1Generation hybrid seeds; f to be obtained1The generation hybrid seeds are respectively and continuously backcrossed with a base material RB for a plurality of times to obtain varieties with strong resistance to the blood type pyosis, which are respectively marked as 881RB and 882 RB; after 5 days of 5-year-old rearing in the spring silkworm stage, carrying out starvation treatment to establish a plurality of alternative strains; breeding and identifying in autumn, and selecting silkworm strains with multiple silk contents and resistance to blood type running sickness for directional breeding to obtain multiple directional strains; performing matched hybridization on the obtained multiple directed strains; through identifying the disease resistance of filial generation and detecting silk amount, silkworm variety with high silk amount and high resistance to blood type pus is selected. The method combines backcross breeding and hunger treatment, and can quickly and accurately obtain the target variety.
Description
Technical Field
The invention relates to the technical field of silkworm breeding, in particular to a breeding method of silkworm with multiple silks and resistance to blood type running sickness.
Background
Domestic silkworm breeding makes great contribution to promoting the development of the silk industry of the whole country, and the breeding of the silkworms also undergoes the process of gradually developing from small to large, from few to many; the three stages of breeding local variety, introducing foreign variety and breeding into self-bred variety are advanced in sequence and developed continuously. However, in the face of the current industry development, the excellent silkworm variety is the most direct, economic and effective important material basis for obtaining high-quality and high-yield silkworm cocoons in the silkworm industry science and technology.
At present, the breeding of silkworm varieties usually adopts a hybridization technology, and a specific gene of one variety can be introduced into another variety, so that the comprehensive economic character reaches a practical level. However, due to the same variety material, even the variety bred by the hybridization method can hardly reach the level with better comprehensive economic character, in particular to a new variety of the silkworm with the characteristics of high silk yield and disease resistance; for this reason, breeders are seeking various methods to solve the deficiencies of breeding efforts.
Disclosure of Invention
The invention aims to provide a breeding method of a multi-silk-content and anti-Nuclear Polyhedrosis (NPV) silkworm.
The invention provides a breeding method of silkworm with multi-silk content and resistance to blood type running sickness, which comprises the following steps: 1) hybridizing a basic material RB carrying a blood-type sepsis-resistant gene with a middle-line pure strain 881 to obtain (881 as a template)RB)F1881×RBHybrid seeds; 2) hybridizing a basic material RB carrying a blood type pyosis resistant gene with a Japanese pure seed 882 to obtain (882 multiplied by RB) F1882×RBHybrid seeds; 3) (881 × RB) F obtained by step 1)1881×RBHybrid and (882 × RB) F obtained in step 2)1882×RBThe hybrid seeds are respectively and continuously backcrossed for a plurality of times with a base material RB carrying the blood type pyosis resistant gene to obtain varieties with strong resistance to the blood type pyosis, and the varieties are respectively marked as 881RB and 882 RB; 4) feeding 881RB and 882RB in spring rearing stage to 5 th day after 5 th day of age, performing starvation treatment, and establishing a plurality of alternative strains; 5) breeding and identifying a plurality of alternative strains obtained in the step 4) in autumn, and selecting a silkworm strain with multiple silk contents and resistance to the blood type running sickness for directional breeding to obtain a plurality of directional strains; 6) combining a plurality of oriented lines obtained in the step 5) into a hybridization combination in a form of day × middle, day/day × middle, and hybridizing; selecting silkworm varieties with high silk yield and high resistance to the blood type pyosis by identifying the disease resistance of filial generations and detecting the silk yield; no time sequence is defined between the step 1) and the step 2).
Preferably, the number of continuous backcrossing in the step 3) is 4-20.
Preferably, the starvation treatment in the step 4) is to reduce the mass of the mulberry given by the silkworms to 70-90%.
Preferably, the starvation treatment in the step 4) is to be started up 1 to 2 days in advance.
Preferably, the hybridization combination described in step 6) comprises 881RBN×881RBN、881RBN×882RB1·882RBN、881RBN·881RBN×882RB1、881RBN·881RBN×882RB1·882RBN。
Preferably, after the silkworm variety with high silk yield and strong resistance to the blood type running sickness is obtained in the step 6), the method also comprises the steps of carrying out rural trial breeding, variety quality identification, variety approval or registration on the obtained silkworm variety, and breeding the silkworm variety into a target variety.
Preferably, the rural trial breeding is to issue the silkworm variety obtained in the step 6) and the reference silkworm variety to a farmer for trial breeding, and to determine the following parameters: the silkworm breeding method comprises the steps of development process in a silkworm breeding period, cage death rate, total cocoon weight, cocoon layer rate, first-grade cocoon rate, seed-expanding cocoon yield and kilogram cocoon grain number.
The invention has the beneficial effects that: in the method, backcross breeding and hunger treatment are combined in the breeding process of the silkworms, and the improvement of the hybridization breeding method is used for backcross breeding of the multivariate hybrids of the silkworms, and disease-resistant genes RB are introduced into the hybridization and backcross so as to ensure that paired multifilamentary varieties 881 multiplied by 882 carry the disease-resistant genes; the starvation treatment is to select a strain with high conversion rate of the cut tobacco by a method of controlling the mulberry feeding amount or mounting the mulberry in advance; the method disclosed by the invention is used for carrying out starvation treatment in spring, establishing alternative strains, and selecting directional strains with strong disease resistance and high filamentation quantity under the conditions of high temperature and poor leaf quality in autumn; the target variety can be quickly and accurately obtained.
Detailed Description
The invention provides a breeding method of silkworm with multi-silk content and resistance to blood type running sickness, which comprises the following steps: 1) crossing the basic material RB carrying the blood type pyosis resisting gene with the middle line pure seed 881 to obtain (881 × RB) F1881×RBHybrid seeds; 2) hybridizing a basic material RB carrying a blood type pyosis resistant gene with a Japanese pure seed 882 to obtain (882 multiplied by RB) F1882×RBHybrid seeds; 3) (881 × RB) F obtained by step 1)1881×RBHybrid and (882 × RB) F obtained in step 2)1882×RBThe hybrid seeds are respectively and continuously backcrossed for a plurality of times with a base material RB carrying the anti-blood type pyosis gene to obtain varieties with strong resistance to the blood type pyosis, and the varieties are respectively marked as 881RB and 882 RB; 4) feeding 881RB and 882RB in spring rearing stage to 5 th day after 5 th day of age, performing starvation treatment, and establishing a plurality of alternative strains; 5) breeding and identifying a plurality of alternative strains obtained in the step 4) in autumn, and selecting a silkworm strain with multiple silk contents and resistance to the blood type running sickness for directional breeding to obtain a plurality of directional strains; 6) combining a plurality of oriented lines obtained in the step 5) into a hybridization combination in a form of day × middle, day/day × middle, and hybridizing; through identifying the disease resistance of filial generation, detecting silk amount, selecting silkworm with high silk amount and high resistance to nuclear polyhedrosisAnd (4) seed preparation.
In the invention, the 'middle and middle' is the stock of the Chinese system formed by the hybridization of the Chinese system variety and the Chinese system variety; the "day-day" is a stock species of the Japanese system obtained by crossing the Japanese system variety with the Japanese system variety; the 'ZhongzhongxRi-Ri' is a quaternary hybrid obtained by hybridizing a Chinese system stock and a Japanese system stock to obtain four parent hybrids. The hybrid form of silkworm "Zhongxi day" is a binary hybrid; "ZhongzhongxRi" and "ZhongxRi" are three-way hybrids; "Zhongzhongxariri" is a quaternary hybrid, and also Zhongxiao, a form in which a Chinese variety and a European variety are crossed.
In the present invention, a binary cross combination 881 × 882 with a multi-silk quality characteristic is selected as a vector variety, 881 is a medium line variety in the binary cross, and 882 is a Japanese system variety in the binary cross. The invention uses the two varieties of the hybridization combination as carrier varieties, namely uses a middle line variety 881 and a daily line variety 882 as hybrid receptor varieties of a middle line and a daily line respectively. In the present invention, (881 × RB) F is obtained by crossing the basic material RB carrying the anti-hemotopsis gene with the Zhongyuan pure seed 8811881×RBHybrid seeds; hybridizing a basic material RB carrying a blood type pyosis resistant gene with a Japanese pure seed 882 to obtain (882 multiplied by RB) F1882×RBAnd (4) hybridizing. In the invention, the base material RB carrying the anti-blood type running sickness gene is the variety number carrying the anti-blood type running sickness gene. The hybridization method of the present invention is not particularly limited, and a hybridization method that is conventional in the art may be used.
The invention obtains (881 multiplied by RB) F respectively1881×RBHybrid seed and (882 × RB) F1882×RBAfter hybridization, the (881 × RB) F was introduced1881×RBHybrid seed and (882 × RB) F1882×RBThe hybrid seeds are respectively and continuously backcrossed for a plurality of times with a base material RB carrying the blood type pyosis resistant gene to obtain varieties with strong resistance to the blood type pyosis, which are respectively marked as 881RB and 882 RB. The number of continuous backcrossing is preferably 4-20, and more preferably 8-16.
According to the invention, after 881RB and 882RB are obtained, 881RB and 882RB are fed in spring rearing period to 5 th day after 5 th day of age, starvation treatment is carried out, and a plurality of alternative strains are established. The spring silkworm stage is spring, the climate and the leafiness conditions of the spring silkworm stage are good, the pathogeny is few, and the silkworm silk yield is high and stable. The starvation treatment in the invention preferably reduces the mass of the mulberry given by the silkworms to 70-90%, and more preferably 80%; the starvation treatment can also be mounting in advance for 1-2 days, for example, under a certain standard temperature and humidity, 5 th-instar silkworms of a giant silkworm should be mature and grow cocoons on the 7 th day, the starvation treatment is to enable the giant silkworms to be mounted on the 5 th day or the 6 th day, and the silkworms mounted in advance start to spin and cocoons on the 8-9 th-instar silkworms. The starvation treatment aims to influence the expression of silkworm antibacterial peptide, mRNA (messenger ribonucleic acid) of a gene related to a silk gland cell cycle and the like through the starvation treatment so as to influence the silk output and resistance of silkworms. According to the invention, after starvation treatment, male and female individuals with normal and medium cocoon types, fine wrinkles, and full cocoon weight, cocoon shell weight and cocoon shell rate close to the original carrier varieties 881 and 882 are preferably selected as target individuals to establish a plurality of alternative lines. The target individual preferred markers are: 881R0601, 881R0602, 881R0603, 881R0604 … …, 882R0601, 882R0602, 882R0603, 882R0604 … ….
After a plurality of alternative strains are obtained, the obtained alternative strains are bred and identified in autumn, and a silkworm strain with multiple silk contents and capable of resisting the nuclear polyhedrosis is selected for directional breeding to obtain a plurality of directional strains. After the breeding of the silkworms is identified as the stage of crossing and matching in the breeding process of the silkworms, a plurality of alternative strain combinations are adopted for breeding observation, and yield, quality and disease resistance indexes are investigated and recorded. The invention selects the directional strain with strong disease resistance and filamentation under the conditions of high temperature in autumn and poor leaf quality; the target variety can be quickly and accurately obtained. The directional culture in the invention refers to continuous subculture aiming at a certain specific character, and continuous reinforcement of the specific character. For example, a line with a lot of silks is selected from a plurality of alternative lines, and the characteristic of strengthening the silks is continuously cultured to obtain the line with obviously improved silks.
After obtaining a plurality of oriented strains, combining the obtained plurality of oriented strains in a form of day multiplied by middle, day multiplied by middle to form a hybrid combination, and hybridizing; through identifying the disease resistance of filial generation and detecting silk amount, silkworm variety with high silk amount and high resistance to blood type pus is selected. The method preferably selects silkworm varieties with high filamentation, strong blood type pyosis resistance and good combining ability in filial generations. The mating ability described in the present invention is the ability of two hybrid parents to bind relatively. The invention preferably tests the combining ability of homologous breeders in the multi-species and also tests the combining ability of the multi-species to the cross breeders in the cross breeding of the multi-species. The testing of the combining ability in the invention can be realized by adopting the conventional combining ability testing method in the field without other special requirements.
After obtaining silkworm varieties with high silk yield and strong resistance to blood type running sickness, the method preferentially performs rural trial breeding, variety quality identification, variety approval or registration on the obtained silkworm varieties to breed target varieties. The rural trial breeding is to distribute the obtained silkworm varieties and the reference silkworm varieties to farmers for trial breeding, and measure the following parameters: the silkworm breeding method comprises the steps of development process in a silkworm breeding period, cage death rate, total cocoon weight, cocoon layer rate, first-grade cocoon rate, seed-expanding cocoon yield and kilogram cocoon grain number. The purpose of the rural test breeding is to test whether the performance of the obtained silkworm variety is stable in all aspects and whether the silkworm variety can withstand the test of actual production. The properties of the silkworm variety of the invention are superior to or at least the same as the reference silkworm variety. The variety quality identification, variety approval or registration in the invention is a routine procedure for determining new varieties of silkworms in the field, and has no other special requirements.
The breeding method of silkworms with a multi-silk content and resistance to Nuclear Polyhedrosis (NPV) according to the present invention will be described in further detail with reference to the following specific examples, and the technical solutions of the present invention include, but are not limited to, the following examples.
Example 1
Crossing the basic material RB carrying the blood type pyosis resisting gene with the middle line pure seed 881 to obtain (881 × RB) F1881×RBHybrid seeds; hybridizing a basic material RB carrying a blood type pyosis resistant gene with a Japanese pure seed 882 to obtain (882 multiplied by RB) F1882×RBHybrid seeds; 3) with (881 × RB) F1881×RBHybrid seed and (882 × RB) F1882×RBThe hybrid seeds are respectively and continuously backcrossed with a basic material RB carrying the blood type pyosis resistant gene for 16 times to obtain varieties with strong resistance to the blood type pyosis, and the varieties are respectively marked as 881RB and 882 RB; 4) feeding 881RB and 882RB in spring silkworm stage to 5 th day after 5 th day, carrying out starvation treatment, wherein silkworms of 5 th day of giant silkworms are mature and bunch and cocoon on 7 th day under standard temperature and humidity, but bunch on 5 th day or 6 th day (a bunch net is covered on the bunch to prevent the silkworms from jumping out), and silkworms forced to bunch can start spinning and cocoon after 8-9 days; establishing a plurality of alternative strains, selecting male and female individuals with normal and medium cocoon types, fine wrinkles, full cocoon weight, cocoon shell weight and cocoon shell rate close to the original carrier varieties 881 and 882 as target individuals, and marking as: 881R0601, 881R0602, 881R0603, 881R0604, …, …, …, 882R0601, 882R0602, 882R0603, 882R0604, …, …, …; breeding and identifying the obtained multiple candidate strains in autumn, and selecting silkworm strains with multiple silks and resistance to the nuclear polyhedrosis for directional breeding to obtain multiple directional strains; carrying out hybridization on a plurality of targeted lines by combining the forms of day × middle, day/day × middle, day/day × middle; through identifying the disease resistance of filial generation and detecting silk quantity, the silkworm variety with high silk quantity and blood type pyosis resistance is selected and obtained as 881R0601 & 881R0603 (Gui silkworm No. 16 middle line stock) and 882R0601 & 882R0603 (Gui silkworm No. 16 Japanese line stock).
Example 2 characteristics of the new variety of original silkworms obtained by the method of example 1 were investigated.
Control variety: pinus sylvestris (pinus sylvestris × aury species), aury (pinus sylvestris × aury species), new species: 881R0601 & 881R0603 (Chonghu No. 16 middle-series stock) and 882R0601 & 882R0603 (Chonghu No. 16 Japanese stock) as controls.
In the autumn rearing stage of 2016, after the variety character is basically stable, in order to grasp the character of two cross mating stocks of new variety hybrid combination, the Hua series stock 881R0601 & 881R0603 and the Japanese series stock 882R0601 & 882R0603 of the variety are raised, the 10 moth egg laying amount, dead egg amount, non-sperm egg amount and the like are tested and investigated, and the control variety is Cyanine pine (middle series), Haoyue moon (Japanese series), 881R0601 & 881R0603 (middle series) and 882R0601 & 882R0603 (Japanese series) which are raised and investigated by the same method.
Raising silkworm
The hatching period of the original silkworm eggs is simultaneously discharged and accelerated with identification seeds, quality guarantee and the like, and the hatching standard is carried out according to the progressive hatching standard. 0.5g of each variety of the newly hatched silkworms is bred by a common four-breeding method until the number of the newly hatched silkworms in the fourth instar is determined. Each variety is provided with two repeating cells, 400 silkworms in each cell are bred to mature cocoons, each cell is divided into two cocooning frames when cocooning frames are carried out, each cocooning frame is covered with a cocooning frame net, cocoons are collected in 6 days, cocoon coats are peeled off, and various performances such as cocoon quality, cocoon shape, cocoon color and the like are investigated in 7 days. And (3) breeding the moths after cocoon cutting and pupation, randomly mating the cross varieties, and then allowing the single moths to lay eggs, wherein the single moths are used for identifying the laying quality performance of the next generation of silkworm cocoons, and meanwhile, the laying amount and the good egg rate of the single moths are investigated. Meanwhile, after selecting eclosion normal female moths to mate with mating varieties for 4 hours, randomly selecting 10 moths to lay eggs on common seed continuous paper, framing the four sides by a wood frame, paying attention to the moth patrol, not allowing the moth eggs to lay eggs on the wood frame side or outside the continuous paper, and repeating the step of respectively repeating the step of the Hua-series female moths and the Japanese-series female moths for 4 times. The total egg yield, unfertilized eggs, dead eggs and other performances are investigated.
Seed production test
Collecting 2g of ants in each variety, breeding and cocooning according to the common breeding, collecting the hair foot cocoons after cocooning for 3d, flatly spreading the hair foot cocoons in a silk worm foil, investigating breeding results in seven days, selecting the same-uterus cocoons and the next cocoons, cutting the cocoons, identifying the pupas, breeding moths, throwing 120 moths per piece of continuous paper, paying attention to patrol moths, and transferring the continuous paper after full production.
Selecting body color of moth
Selecting 881R0601 and 881R0603 according to conventional varieties in the whole process of catching, mating and throwing the moths, fitting, folding and producing seeds and throwing the moths; 882R0601 and 882R0603 select white moths, which are fully unfolded moths and have regular scale hairs, as seed-producing moths, and exclude non-black moths and bad moths.
The spawning property tests are carried out according to the conditions that the humidity is 80%, the spawning temperature is 25 ℃, the humidity is about 75%, the open-light mating and the dark protection spawning are carried out.
Analysis of results
The time of breeding and development of silkworm and moth
The breeding and development process and the moth-raising time of the original silkworm are shown in table 1, and as can be seen from table 1, the age of the new variety of the original silkworm is shorter than that of the original silkworm in pinkish and gosteme, and is equivalent to 881R0601 & 881R0603 and 882R0601 & 882R 0603. The moth-shooting time is about 6 to 9 am, and the concentrated moth-shooting time is 7 in am: about 30, which is beneficial to the seed production by the conventional method and saves time and labor input.
TABLE 1 Gui silkworm No. 16 original silkworm rearing and moth-developing achievement table
Health and seed production performance of silkworm breeding
In terms of health, as can be seen from table 2, the pupa rate of the Guihua series variety 881R0601 & 881R0603 of Guihua No. 16 reaches 99.89%, is 1.73% higher than that of Cyanin pine, and is 1.00% higher than that of 881R0601 & 881R 0603; the pupa rate of the Japanese variety 882R0601 & 882R0603 is 99.93%, which is 2.04% higher than Haoyue, and 1.80% higher than 882R0601 & 882R 0603. The common cocoon rate is also higher, the common cocoon rate of the Huashi 881R0601 & 881R0603 reaches 98.59 percent, is 0.18 percent smaller than that of cyanine pine, and is 0.39 percent smaller than that of the 881R0601 & 881R 0603; the 882R0601 & 882R0603 of the Japanese system reaches 98.70 percent, is 1.02 percent higher than the moon and is 0.03 percent smaller than the 882R0601 & 882R 0603. In the aspect of cocoon quality, the total cocoon weight of Hua series 881R0601 & 881R0603 reaches 1.68g, the cocoon layer weight reaches 0.40g, the cocoon layer rate reaches 23.81%, and the cocoon layer rate is respectively 0.03g higher than that of cyanine pine, 0.0g and 0.14 percent lower than that of cyanine pine, and is completely equal to the total cocoon weight, the cocoon layer weight and the cocoon layer rate of 881R0601 & 881R 0603; the total cocoon weight of the Japanese series 882R0601 and 882R0603 is 1.69g, the cocoon shell weight is 0.39g, the cocoon shell rate is 23.67%, which is respectively 0.02g higher than Haoyue, 0.0g lower than Haoyue and 0.21 percentage point lower than Haoyue, and 0.02g higher than 882R0601 and 882R0603, 0.0g lower than 882R0601 and 882R0603, respectively, and 0.28 percentage point lower than Haoyue.
As a result of selecting the color of the moth, the 882R0601 and 882R0603 black moth rate is high, the moth body is normal, and the health is good.
TABLE 2 breed-making achievement table for Gui silkworm No. 16 breed
As can be seen from Table 2, the ratio of Gui silkworm No. 16 to Jing pine, Haoyue and 881R0601 & 881R0603, 882R0601 & 882R0603 is 3.81 pieces for Jing pine, 881R0601 & 881R0603 is 4.1 pieces for 881R0601 & 881R0603 and 3.74 pieces for the China series of kilogram cocoon production, and the scores of the new variety approach two control varieties; 3.65 in Haoyue of the Japanese system, 4.2 in 882R0601 & 882R0603 and 3.83 in 882R0601 & 882R0603, and the new variety score is higher than Haoyue and is close to 882R0601 & 882R 0603. 15.5 pinus cyanine of the Chinese series for termite breeding, 16.0 pinus cyanine of 881R0601 & 881R0603 and 15.5 pinus cyanine of 881R0601 & 881R0603, wherein the breeding performance of the new species is close to that of two control species; in the Japanese system, 15.25 pieces of moon, 16.5 pieces of 882R 0601. 882R0603 and 15.7 pieces of 882R 0601. 882R0603 are observed, and the new variety is higher than that in the Japanese system and is close to 882R 0601. 882R 0603.
In order to further analyze the difference significance of the performances among various varieties, the variance analysis (not shown in the table) and multiple comparison are carried out on the pupa rate, the cocoon yield and the total cocoon yield with the most representative characteristics in the cocoon quality, which are main characteristics of stress resistance in raising, the cocoon yield and the cocoon yield, in the table 3 and the table 4. As can be seen from Table 3, the pupa rate has the magnitude sequence of 882R0601 & 882R0603 > 881R0601 & 881R0603 > cyanine pine > 882R0601 & 882R0603 > Haoyue, and the new combination stock 881R0601 & 881R0603 and 882R0601 & 882R0603 are very different from cyanine pine (P is less than 0.01). The comparison of the whole cocoon weight of the original silkworm is shown in the table 4, the size sequence is 882R0601 & 882R0603 > 881R0601 & 881R0603 > 882R0601 & 882R0603 > Haoyue > Cyanine pine, and the whole cocoon weight of 2 original species of the new combination is different from that of Haoyue but is not obvious (P < 0.05).
Therefore, the original characteristics of the noble silkworm 16 variety are stable, and all the characteristics are close to or exceed those of the control variety, thereby achieving the practical level.
TABLE 3 multiple comparison of pupa rates of breeder seeds 881R0601 & 881R0603 and 882R0601 & 882R0603
Note: in the table, significant differences are indicated, and extremely significant differences are indicated.
TABLE 4 multiple comparisons of the total cocoon weight of the stock seeds 881R0601 & 881R0603, 882R0601 & 882R0603
Result of primary silkworm spawning
The results of the investigation of the egg laying amount and the egg quality of the 10 moths are shown in the table 5, and it can be seen that the egg laying amount of 881R0601 and 881R0603 averagely reaches 486 granules, and the egg laying rate reaches 97.03%; the total egg yield of 882R0601 and 882R0603 reaches 445 grains, and the good egg rate is 97.51%. Therefore, whether the variety is a Hua series or a Japanese series stock, the egg laying amount is large, the 10 moth egg laying amount is relatively average, the variety property is relatively stable, and the result is consistent with the result related to the conventional variety egg laying amount research.
TABLE 5 spawning survey of the stock species 881R0601 & 881R0603, 882R0601 & 882R0603 female moths
The breeding target of practical silkworm varieties requires easy breeding of silkworm, namely, the resistance of the original silkworm is stronger, the common cocoon rate is higher, and the seed production quantity of the termite-killing silkworm is higher. The cocoon quality requirement is excellent, which not only directly relates to the seed production quantity, but also influences the cocoon silk quality of hybrid offspring (common seed), especially the cocoon layer rate is high, and the cocoon layer rate is higher for the next generation.
From the result analysis of the original silkworm breeding, the variety of Gui silkworm No. 16 stock 881R0601 & 881R0603 and 882R0601 & 882R0603 show the characteristics of stronger resistance, higher common cocoon rate and better cocoon quality, thereby not only meeting the requirement of easy breeding of silkworm, but also achieving the next-generation achievement of better comprehensive properties.
The new variety Gui silkworm No. 16 (881R0601 & 881R0603, 882R0601 & 882R0603) with disease-resistant gene shows that the original silkworm breeding performance is regular development, good health, stronger stress resistance, higher cocoon yield and better cocoon quality. The color of male and female moths of 882R0601 and 882R0603 is gray, and the color of the moths is consistent; after the female moths are mated with the mating varieties, the female moths lay eggs earlier and more intensively, the egg laying amount is more, and the egg quality is excellent. Compared with the seed production test of the positive and negative cross of the contrast variety, the seed production quantity of the termite control is close to that of the contrast variety. According to all the results, the noble silkworm 16 is a pair of inbred stock with excellent comprehensive character, the stock character keeps the excellent production performance of the original variety noble silkworm 16 parent (881R0601 & 881R0603, 882R0601 & 882R0603) except the character with black moth mark, the egg laying amount is large, the egg color is consistent, and the hybrid is a pair of practical multielement hybrid new varieties with disease-resistant genes.
Example 3 rural pilot-plant trial of a new variety obtained by the method of example 1.
Test for rural test cultivation
1.1 test materials
The tested silkworm variety is screened from the identified disease-resistant material to be the new combined noble silkworm No. 16 (district test number), and the reference silkworm variety No. 2 is screened.
1.2 test methods
The tested silkworm variety and the reference variety are exported uniformly, and the incubation, the ant harvesting and the young silkworm co-breeding are performed at the co-breeding points of all demonstrations. The test and control species were distributed to each tester one day after being bred to the food at the 3 rd rate at 2 g/foil. The technical level is selected by the experimenter, the mulberry tree planting varieties are consistent, the planting time is consistent, and the facility equipment is relatively consistent for 3 farmers. The same technology is adopted by each experimental farmer for breeding the silkworm as much as possible, and 3-day return breeding is adopted for the whole age. In order to improve data accuracy comparatively, cocooning is carried out 6 days after cocooning of silkworms, 3 samples are randomly extracted from a batch of concentrated cocoons on 7 days, each sample is 3kg, 9 kg of silkworm cocoons are fully mixed and then extracted 3 samples, and each sample is 1 kg of silkworm cocoons to serve as survey sample cocoons. Full-term survey items: the silkworm breeding method comprises the steps of development process in a silkworm rearing period, cage death rate, total cocoon quantity, cocoon layer rate, first-grade cocoon rate (the first-grade cocoon is determined according to the purchasing standard of a company), cocoon yield of a strain and the number of kilogram cocoons. The investigation of various performances of the silkworm variety is the same as that of the test species.
2.2.1 results and analysis
2.2.1 rural trial achievement
2.2.1.1 Weining county gold bucket Zhen Hei nicun
From table 6, in spring, the Guifei No. 16 silkworm is bred in the rural area with the highest elevation of Guizhou, namely Weining county (the elevation 2200m) and the Jindouzhen town (the elevation 1700m) of Weining county in the highest elevation of Guizhou, the death rate is reduced by 10.17 percentage points compared with the control, the total cocoon weight is 1.91g, the cocoon shell weight is 0.44g, and the cocoon shell rate is 23.17%, and the death rate is respectively increased by 11.05%, 15.99% and 0.93 percentage points compared with the control; the first-class cocoon rate is 98.50 percent, which is improved by 1.18 percent compared with the control; the average cocoon yield of the strain is 42.47kg, which is 11.04 percent higher than that of the control. The yield of the strain is 1756.71 yuan, which is increased by 13.12 percent compared with the control.
TABLE 6 comparison of Gui silkworm No. 16 with Gui Zhou silkworm No. 2
(2015, 2016 average performance in spring and silkworm period-weining), altitude: 1700m
From table 7, in summer, the noble silkworm 16 is bred in the Jindou town of Weining county, the death rate is reduced by 9.77 percentage points compared with the control, the total cocoon weight is 1.90g, the cocoon shell weight is 0.43g, the cocoon shell rate is 22.66%, and the death rate is respectively improved by 10.16%, 11.69% and 0.31 percentage points compared with the control; the first-class cocoon rate is 97.22 percent, which is improved by 0.91 percent compared with the control; the average cocoon yield of the strain is 42.15kg, which is 10.48 percent higher than that of the control. The yield of the strain is 1741.66 yuan, which is increased by 11.54% compared with the control.
TABLE 7 comparison of Gui silkworm No. 16 and Gui Zhou silkworm No. 2
(2015, 2016 average performance in silkworm summer-Weining), altitude: 1700m
From the results of tables 6 and 7, in the high-altitude silkworm area, the noble silkworm No. 16 still has good adaptability, high yield and excellent cocoon quality performance, and shows high yield performance. In a silkworm area with the altitude of more than 1700 meters, the noble silkworm No. 16 has good adaptability and shows high yield performance. Rarely has the occurrence of the running sickness, regular development, good nourishment and regular aging and clustering. The cocoon quality performance is excellent, and the cocoon yield value of a single-seed are obviously improved compared with those of a control variety. And is the silkworm area with the highest cocoon quality and yield in each test area. The fact that the variety is in a plateau silkworm area and the bred Guiyan No. 16 silkworm is a silkworm variety which has good adaptability, high yield and health.
The technical key points of breeding disease-resistant varieties in plateau silkworm areas are that ① in the spring silkworm period emphasizes heat preservation at night and reduces temperature difference between day and night, ② silkworm households strictly sterilize the environment before, in and after silkworms, emphasizes silkworm tool sterilization, ③ strictly washes hands, supplies mulberry, changes shoes and enters rooms, and strictly uses the silkworm tool in different areas.
2.2.1.2 Huangping county
From table 2, the Guifei No. 16 silkworm is raised in a silkworm area with an altitude of about 640 in Guizhou in spring, the death rate is reduced by 11.82% compared with the control, the total cocoon weight is 1.90g, the cocoon shell weight is 0.43g, the cocoon shell rate is 22.79%, and the death rate is respectively improved by 13.96%, 9.31% and 1.02% compared with the control; the first-class cocoon rate is 97.13 percent, which is improved by 1.04 percent compared with the control; the average cocoon yield of the strain is 41.54kg, which is increased by 15.20% compared with the control. The yield of the strain is 1694.71 yuan, which is increased by 16.44% compared with the control.
TABLE 8 comparison of Gui silkworm No. 16 with Gui Zhou silkworm No. 2
(2015, 2016 average spring and silkworm performance-Huangping), altitude: 640m
From table 8, the noble silkworm 16 # is bred in summer in rural areas in Huangping county, the death rate is reduced by 12.32 percent compared with the control, the total cocoon weight is 1.88g, the cocoon shell weight is 0.43g, the cocoon shell rate is 22.93 percent, and the death rate is respectively improved by 12.61 percent, 18.62 percent and 1.16 percent compared with the control; the first-grade cocoon rate is 96.39%, which is improved by 0.30 percentage points compared with the control; the average cocoon yield of the strain is 40.73kg, which is 12.96 percent higher than that of the control. The yield of the strain is 1668.97 yuan, which is increased by 13.31 percent compared with the control.
Table 9 the breeding of Gui silkworm No. 16 and the control Guizhou silkworm No. 2 (2015, 2016 average summer silkworm period-Huangping), altitude: 640m
From the results in tables 8 and 9, in the middle-high-altitude silkworm area of southeast Guizhou province in Guizhou, the Guizhou No. 16 silkworm has high yield and excellent cocoon quality performance, and shows high yield performance. Rarely has the occurrence of the running sickness, regular development, good nourishment and regular aging and clustering. The cocoon quality performance is excellent, and the cocoon yield value of a single-seed are obviously improved compared with those of a control variety. The technical points are as follows: the anti-stiff powder is reasonably used, and the disinfection and the requirement before, during and after the silkworm are strict, so that the paid service is well done by the county-related departments matched with the county farm-herding bureau matched with the county development and improvement committee, the county farm-herding bureau and other multiple departments.
2.2.1.3 Lipbo county Maolanzhen
From Table 10, Gui silkworm No. 16 was bred in spring in the low-altitude rural areas such as Yaoyangcun and Yaming village in Maolan town, Libo county. The cage death rate is reduced by 7.43 percent compared with the control, the total cocoon weight is 1.84g, the cocoon shell weight is 0.43g, the cocoon shell rate is 23.20 percent, and the cage death rate is respectively improved by 12.35 percent, 22.85 percent and 2.01 percent compared with the control; the first-class cocoon rate is 98.20 percent, which is improved by 1.39 percentage points compared with the control; the average cocoon yield of the strain is 40.78kg, which is 13.97 percent higher than that of the control. The yield of the strain is 1701.97 yuan, which is 15.62 times higher than that of the control.
TABLE 10 comparison of Gui silkworm No. 16 with the control Gui Guang No. 2 Guizhou silkworm rearing
(2015, 2016 average performance in spring and silkworm period-litchi), altitude: 425m
From table 11, in summer, when the noble silkworms No. 16 are bred in villages such as Yao Anguilun village and Yao Mingcun village in Libo orchid, the death rate is reduced by 11.22 percentage points compared with the control, the total cocoon weight is 1.87g, the cocoon shell weight is 0.43g, the cocoon shell rate is 23.03%, and the death rate is respectively improved by 13.33%, 19.44% and 1.18 percentage points compared with the control; the first-grade cocoon rate is 96.39%, which is improved by 0.30 percentage points compared with the control; the average cocoon yield of the strain is 40.57kg, which is 13.74 percent higher than that of the control. The yield of the strain is 1662.47 yuan, which is 14.06% higher than that of the control.
TABLE 11 comparison of Gui silkworm No. 16 with Gui Zhou silkworm No. 2
(2015, 2016 average performance in summer rearing period-litchi), altitude: 425m
From the results of tables 10 and 11, in a low-altitude silkworm area of Guizhou with an altitude of about 400m, Guizhou No. 16 silkworm also has good adaptability, shows high yield and has high cocoon quality. Rarely has running into the pus, the stubborn disease, etc., has good development uniformity, good nourishment, and regular aging and clustering. The cocoon quality performance is excellent, and the cocoon yield value of a single-seed are obviously improved compared with those of a control variety. The method proves that the bred Guiyan No. 16 silkworm is a healthy silkworm variety with good adaptability and high yield in the low-altitude silkworm area of Guizhou.
The application in low-altitude silkworm rearing areas is characterized in that the anti-stiff powder is fully used, and the anti-stiff powder is used for silkworm rearing beds on the day of rain. The attention is paid to the ventilation of the silkworm house and the disinfection of each link is strict.
From the above embodiments, the comparison analysis of the new variety of the precious silkworm 16 obtained by the method for breeding silkworms of the present invention in the 400m elevation silkworm areas of the Guizhou with the elevation of 1700m, the elevation of 600m and the elevation of 400m using the two Guangdan No. two as the reference shows that the precious silkworm 16 has no obvious difference in each silkworm area in spring and summer. The death rate is very little developed in spring and summer, the incidence rate is obviously lower than that of a control variety, and the adaptability is strong. When the noble silkworms are bred in various silkworm areas, the yield of the noble silkworms No. 16 is high, the growth is neat, the cocoon quality is excellent, the performance is superior to that of a control species, and the method is suitable for large-area popularization and application in different altitude silkworm areas of Guizhou.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A breeding method of silkworm with multiple silks and capable of resisting blood type pyosis comprises the following steps:
1) hybridizing a basic material RB carrying a blood-type pyosis-resistant gene with a middle-line pure seed 881 to obtain a (881 multiplied RB) F1881 multiplied RB hybrid;
2) hybridizing a basic material RB carrying a blood type pyosis resistant gene with a Japanese pure seed 882 to obtain a (882 × RB) F1882 × RB hybrid;
3) continuously backcrossing (881 xRB) F1881 xRB hybrid seeds obtained in the step 1) and (882 xRB) F1882 xRB hybrid seeds obtained in the step 2) with a base material RB carrying a blood-type pyosis resistance gene for a plurality of times respectively to obtain varieties with strong resistance to the blood-type pyosis, wherein the varieties are marked as 881RB and 882RB respectively;
4) feeding 881RB and 882RB obtained in the step 3) in spring rearing, and after feeding to 5 th day of age, carrying out starvation treatment to establish a plurality of alternative strains; the starvation treatment is to reduce the mass of the mulberry given by the silkworms to 70-90% of the mass of the mulberry to be given by the variety or to mount the mulberry in advance for 1-2 days;
5) breeding and identifying a plurality of alternative strains obtained in the step 4) in autumn, and selecting a silkworm strain with multiple silk contents and resistance to the blood type running sickness for directional breeding to obtain a plurality of directional strains;
6) combining a plurality of oriented strains obtained in the step 5) into a hybridization combination for hybridization; selecting silkworm varieties with high silk yield and high resistance to the blood type pyosis by identifying the disease resistance of filial generations and detecting the silk yield; wherein "middle" is a Chinese phylogenetic species, "day" is a Japanese phylogenetic species, ". and" x "represent a cross; the hybrid combination comprises 881RBN × 881RBN, 881RBN × 882RB1 & 882RBN, 881RBN & 881RBN × 882RB1, 881RBN & 881RBN × 882RB1 & 882 RBN;
no time sequence is defined between the step 1) and the step 2).
2. A breeding method according to claim 1, characterized in that the number of successive backcrosses in step 3) is 4 to 20.
3. A breeding method as claimed in claim 1, wherein after obtaining silkworm variety with high filamentation and strong resistance to nuclear polyhedrosis in step 6), the method further comprises the steps of performing rural test breeding, variety quality identification, variety approval or registration on the obtained silkworm variety to breed the target variety.
4. A breeding method according to claim 3, characterized in that the rural test rearing is that the silkworm variety obtained in step 6) and the control silkworm variety are issued to farmers for test rearing, and the following parameters are measured: the silkworm breeding method comprises the following steps of (1) developing and cage-death rate in a silkworm rearing period, total cocoon quantity, cocoon layer rate, primary cocoon rate, seed-expanding cocoon yield and kilogram cocoon grain number; the control silkworm variety is pinus sylvestris or gosteme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711238086.1A CN107821342B (en) | 2017-11-30 | 2017-11-30 | Breeding method of silkworm with multiple silks and capable of resisting blood type pyosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711238086.1A CN107821342B (en) | 2017-11-30 | 2017-11-30 | Breeding method of silkworm with multiple silks and capable of resisting blood type pyosis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107821342A CN107821342A (en) | 2018-03-23 |
| CN107821342B true CN107821342B (en) | 2020-04-14 |
Family
ID=61646789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711238086.1A Active CN107821342B (en) | 2017-11-30 | 2017-11-30 | Breeding method of silkworm with multiple silks and capable of resisting blood type pyosis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107821342B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114698604B (en) * | 2022-04-07 | 2023-07-18 | 辽宁省蚕业科学研究所 | Method for breeding tussah larvae with hunger resistance and nutritional stress |
| CN116671499B (en) * | 2023-06-28 | 2024-06-14 | 吉林省蚕业科学研究院(吉林省农特产品研究院、吉林省北冬虫夏草研究中心) | Tussah comprehensive hybrid seed creation method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101107925A (en) * | 2007-08-27 | 2008-01-23 | 贵州省蚕业研究所 | Multicomponent cross breeding method of selection and breeding of silkworm |
| CN101270365A (en) * | 2008-04-30 | 2008-09-24 | 苏州大学 | Method for preparing transgenic cultivated silkworm with high resistance for blood type nuclear polyhedrosis |
| CN103081872A (en) * | 2013-02-04 | 2013-05-08 | 江苏科技大学 | Method for breeding double-limitation multi-silk-quantity domestic silkworm breed |
| CN103190381A (en) * | 2013-04-28 | 2013-07-10 | 贵州省蚕业研究所 | Breeding method of multivariate hybrids of double backcross breeding silkworm |
| CN104839099A (en) * | 2015-04-17 | 2015-08-19 | 江苏科技大学 | Method for improving silkworm nuclear polyhedrosis resistance through hybridization and backcross |
| CN105145500A (en) * | 2015-06-30 | 2015-12-16 | 江苏科技大学 | Bombyxmori Nucleopolyhedrovirus ( BmNPV) full-resistance breeding method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140189899A1 (en) * | 2013-01-03 | 2014-07-03 | Sadia "Ross" Barrameda | Methods of producing new types of hybrid silk and fibers using insects, animals, and plants |
-
2017
- 2017-11-30 CN CN201711238086.1A patent/CN107821342B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101107925A (en) * | 2007-08-27 | 2008-01-23 | 贵州省蚕业研究所 | Multicomponent cross breeding method of selection and breeding of silkworm |
| CN101270365A (en) * | 2008-04-30 | 2008-09-24 | 苏州大学 | Method for preparing transgenic cultivated silkworm with high resistance for blood type nuclear polyhedrosis |
| CN103081872A (en) * | 2013-02-04 | 2013-05-08 | 江苏科技大学 | Method for breeding double-limitation multi-silk-quantity domestic silkworm breed |
| CN103190381A (en) * | 2013-04-28 | 2013-07-10 | 贵州省蚕业研究所 | Breeding method of multivariate hybrids of double backcross breeding silkworm |
| CN104839099A (en) * | 2015-04-17 | 2015-08-19 | 江苏科技大学 | Method for improving silkworm nuclear polyhedrosis resistance through hybridization and backcross |
| CN105145500A (en) * | 2015-06-30 | 2015-12-16 | 江苏科技大学 | Bombyxmori Nucleopolyhedrovirus ( BmNPV) full-resistance breeding method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107821342A (en) | 2018-03-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Tong et al. | Capsicum tovarii, a new member of the Capsicum baccatum complex | |
| CN102318571B (en) | Culture method of hybrid new variety of Argopecten irradias and Argopecten purpuratus | |
| CN102301971B (en) | Method for breeding novel hybrid scallop variety of Argopecten purpuratus and Argopecten irradians irradians | |
| Tikader et al. | Muga silkworm, Antheraea assamensis (Lepidoptera: Saturniidae)-an overview of distribution, biology and breeding. | |
| CN107801631B (en) | Heat conduction temperature corn germplasm penetration type breeding improvement method | |
| CN102440179A (en) | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding | |
| CN108967185B (en) | Breeding method of high-quality rice blast resistance strong-advantage indica rice restorer line | |
| CN111727939B (en) | Method for breeding new silkworm variety suitable for artificial feed breeding of young silkworms in south subtropical regions | |
| CN105981690B (en) | A kind of meat goose selection | |
| Tikader et al. | Mulberry wild species in India and their use in crop improvement-a review. | |
| CN102499060A (en) | Transgenic helicoverpa armigera-resistant and phenol-free cotton breeding method | |
| CN107821342B (en) | Breeding method of silkworm with multiple silks and capable of resisting blood type pyosis | |
| CN113841610A (en) | Efficient breeding method of fragrant rice blast resistant rice with high whole rice percentage | |
| Gunasekaran et al. | Cotton CO17-A short duration, high yielding compact variety suitable for high density planting system | |
| CN110169355B (en) | Cultivation method of summer direct seeding cotton variety | |
| CN101361467B (en) | Common intertidal shellfish hybridization method | |
| CN114208735A (en) | Method for cultivating rapid-growth new strain of hong Kong oyster triploid by backcross breeding technology | |
| CN112790164B (en) | Breeding method of special silkworm variety suitable for industrial full-age artificial feed breeding | |
| RU2650764C2 (en) | Corn products and methods of their obtaining | |
| CN114698604B (en) | Method for breeding tussah larvae with hunger resistance and nutritional stress | |
| CN114027269B (en) | Low-temperature stress induced resistance breeding method for tussah larva | |
| CN115633635A (en) | Method for creating sweet corn DH line based on haploid breeding technology | |
| Khudjamatov et al. | Intensity of egg laying dynamics by butterflies in the first day of the caterpillar’s life’s period and their relationship with the silkworm selection characteristics | |
| CN107027718A (en) | The method that Diapause transgenic bombyx mori is made by instant corona | |
| CN103749401B (en) | Practical silkworm variety cultivation method capable of controlling male ratio and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |