CN107821342A - A kind of multifibres amount, the breeding method of anti-nuclear polyhedrosis silkworm - Google Patents
A kind of multifibres amount, the breeding method of anti-nuclear polyhedrosis silkworm Download PDFInfo
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Abstract
The present invention relates to a kind of multifibres amount, the breeding method of anti-nuclear polyhedrosis silkworm, belong to silkworm breeding technical field, comprise the following steps：It is purebred 881, Japanese purebred 882 to hybridize respectively with the basic material RB for carrying anti-nuclear polyhedrosis gene, acquisition F1For cenospecies；By the F of acquisition1For cenospecies respectively with basic material RB continuous backcrosses several times, obtain the kind to nuclear polyhedrosis resistance, be designated as 881RB and 882RB respectively；Nature enemy is carried out after spring silkworm rearing season raises the 5th day 5 ages, establishes multiple alternative strains；Raising identification is carried out in the fall, is selected the silkworm strain of multifibres amount and anti-nuclear polyhedrosis to be oriented cultivation, is obtained multiple orientation strains；Multiple orientation strains of acquisition are carried out assembling hybridization；By identifying disease resistance, the detection silk amount of filial generation, selection obtains the strong cultivated silkworm breed variety of multifibres amount, anti-nuclear polyhedrosis.This method is combined backcross breeding with Nature enemy, can fast and accurately obtain target variety.
Description
Technical field
The present invention relates to silkworm breeding technical field, and in particular to the breeding of a kind of multifibres amount, anti-nuclear polyhedrosis silkworm
Method.
Background technology
To promoting whole national Silk Industry development to make major contribution, the breeding of silkworm also experienced China's silkworm breeding
From small to large, from less to more, the process progressively developed；By raising local varieties-Introduced From Abroad kind-incubation voluntarily product
In these three stages of kind, step by step, continue to develop.But in face of the great development of current industry, excellent Silkworm varieties are sericulture science and technology
In it is most direct, most economical, most effective acquisition good quality and high output silk cocoon important substance basis.
The seed selection of cultivated silkworm breed variety at present generally all uses hybridization technique, a kind of specific gene of kind can be imported another
In kind, integral economic trait is set to reach practical level.But due to duplicating for kind material, i.e., it is bred as using hybridizing method
Kind is also difficult also to reach that integral economic trait is preferably horizontal, especially has that silk amount is high and the silkworm of disease resistance feature is new concurrently simultaneously
Kind；Therefore, breeding scholar is insufficient present in breeding work to solve seeking various methods.
The content of the invention
It is an object of the invention to provide a kind of multifibres amount, the breeding method of anti-nuclear polyhedrosis (NPV) silkworm.
The invention provides a kind of multifibres amount, the breeding method of anti-nuclear polyhedrosis silkworm, comprise the following steps：1) with taking
Basic material RB with anti-nuclear polyhedrosis gene is purebred 881 hybridization with, obtains (881 × RB) F1881×RBCenospecies；2)
With the basic material RB and Japanese purebred 882 hybridization for carrying anti-nuclear polyhedrosis gene, (882 × RB) F is obtained1882×RBCenospecies；
3) (881 × RB) F obtained with step 1)1881×RB(882 × RB) F that cenospecies and step 2) obtain1882×RBCenospecies is distinguished
Basic material RB continuous backcrosses with carrying anti-nuclear polyhedrosis gene obtain the product to nuclear polyhedrosis resistance several times
Kind, 881RB and 882RB are designated as respectively；4) Nature enemy is carried out after spring silkworm rearing season raising 881RB and 882RB to the 5th day 5 ages, built
Found multiple alternative strains；5) the multiple choices for being obtained step 4) tie up to autumn and carry out raising identification, select multifibres amount simultaneously
And the silkworm strain of anti-nuclear polyhedrosis is oriented cultivation, multiple orientation strains are obtained；6) it is multiple fixed step 5) to be obtained
Carry out day to strain × in, everyday × in, day × in, everyday × middle form combines cross combination, carries out
Hybridization；By identifying disease resistance, the detection silk amount of filial generation, selection obtains the strong silkworm product of multifibres amount, anti-nuclear polyhedrosis
Kind；Limited between the step 1) and step 2) without time sequencing.
Preferably, the number of the continuous backcross described in step 3) is 4~20 times.
Preferably, the Nature enemy described in step 4) is that silkworm is reduced into original 70~90% to Sang Zhiliang.
Preferably, the Nature enemy described in step 4) is to be placed on small straw bundles to spin cocoons for 1~2 day in advance.
Preferably, the cross combination described in step 6) includes 881RBN×881RBN、881RBN×882RB1·882RBN、
881RBN·881RBN×882RB1、881RBN·881RBN×882RB1·882RBN。
Preferably, after step 6) obtains the strong cultivated silkworm breed variety of multifibres amount, anti-nuclear polyhedrosis, in addition to the silkworm to acquisition
Kind carries out field trial, variety and quality identification, variety certification or registration, is bred as target variety.
Preferably, the field trial is that the cultivated silkworm breed variety for obtaining step 6) is issued to peasant household with compareing cultivated silkworm breed variety and entered
Row examination is supported, and determines following parameter：Big silkworm rearing season developmental duration, dead cage rate, cocoon weight, cocoon shell weight, Cocoon layer ratio, one-level cocoon rate,
The kind amount of cocoon production and kilogram cocoon grain number.
Beneficial effects of the present invention：Method of the present invention is in silkworm breeding process, at backcross breeding and starvation
Reason combines, and backcross breeding silkworm polyspecific hybrids are that cross breeding method is improved, hybridization and Backcross introgression disease-resistant gene
RB, it is ensured that paired multifibres amount kind 881 × 882 carries disease-resistant gene；Nature enemy is then by controlling to mulberry amount or carrying
Before the method be placed on small straw bundles to spin cocoons, select the high strain of conversion rate of silk leaves；Method of the present invention carries out Nature enemy in spring, establishes
Alternative strain, selects that disease resistance is strong, the orientation strain of multifibres amount under conditions of high temperature and the food value of leaf are poor in the fall；Can quickly,
Accurately obtain target variety.
Embodiment
The invention provides a kind of multifibres amount, the breeding method of anti-nuclear polyhedrosis silkworm, comprise the following steps：1) with taking
Basic material RB with anti-nuclear polyhedrosis gene is purebred 881 hybridization with, obtains (881 × RB) F1881×RBCenospecies；2)
With the basic material RB and Japanese purebred 882 hybridization for carrying anti-nuclear polyhedrosis gene, (882 × RB) F is obtained1882×RBCenospecies；
3) (881 × RB) F obtained with step 1)1881×RB(882 × RB) F that cenospecies and step 2) obtain1882×RBCenospecies is distinguished
Basic material RB continuous backcrosses with carrying anti-blood group pus illness gene obtain the kind to nuclear polyhedrosis resistance several times,
881RB and 882RB are designated as respectively；4) Nature enemy is carried out after spring silkworm rearing season raising 881RB and 882RB to the 5th day 5 ages, established
Multiple alternative strains；5) the multiple choices for being obtained step 4) tie up to autumn and carry out raising identification, select multifibres amount and
The silkworm strain of anti-nuclear polyhedrosis is oriented cultivation, obtains multiple orientation strains；6) the multiple orientations obtained to step 5)
Strain carries out day × in, everyday × in, day × in, everyday × middle form combines cross combination, carries out miscellaneous
Hand over；By identifying disease resistance, the detection silk amount of filial generation, selection obtains the strong cultivated silkworm breed variety of multifibres amount, anti-nuclear polyhedrosis.
In the present invention, described " in " turns into Chinese system for Chinese system kind and Chinese system mixing breed
Original seed；" everyday " is the original seed that Japanese system kind turns into Japanese system with Japanese system mixing breed；Described " in
In × everyday " hybridize for Chinese system original seed and Japanese system original seed, obtain the tetra hybrid of four parents.Silkworm
Hybrid versions " in × day " be two-way cross kind；" in × day ", " in × everyday " it is three way cross kind；" in
× everyday " it is tetra hybrid, in also having × Europe, i.e. Chinese variety and the form of European varieties hybridization.
In the present invention, two-way cross combination 881 × 882 of the selection with multifibres flow characteristic is carrier kind, and 881 be two
It is kind in member hybridization, 882 be the Japanese system kind in two-way cross kind.The present invention is with two of this cross combination
Kind is carrier kind, i.e., system and Japanese hybrid receptor kind in being respectively with middle system's kind 881 and Japanese kind 882.
In the present invention, it is purebred 881 hybridization with the basic material RB of the anti-nuclear polyhedrosis gene of carrying, obtains (881 × RB)
F1881×RBCenospecies；With the basic material RB and Japanese purebred 882 hybridization for carrying anti-nuclear polyhedrosis gene, obtain (882 × RB)
F1882×RBCenospecies.In the present invention, the basic material RB for carrying anti-nuclear polyhedrosis gene is with anti-blood type purulence
The kind numbering of ospc gene.The present invention is not particularly limited to the hybridizing method, using the conventional hybridizing method in this area.
The present invention is obtaining (881 × RB) F respectively1881×RBCenospecies and (882 × RB) F1882×RBAfter cenospecies, by described in
(881 × RB) F1881×RBCenospecies and (882 × RB) F1882×RBCenospecies is respectively with carrying the base of anti-nuclear polyhedrosis gene
Plinth material RB continuous backcrosses several times, obtain the kind to nuclear polyhedrosis resistance, are designated as 881RB and 882RB respectively.This hair
The number of continuous backcross described in bright is preferably 4~20 times, more preferably 8~16 times.
It is hungry to progress after the 5th day 5 ages that the present invention raises 881RB and 882RB after 881RB and 882RB is obtained, in spring silkworm rearing season
Processing is starved, establishes multiple alternative strains.Heretofore described spring silkworm rearing season is spring, the weather and food value of leaf condition of the spring silkworm rearing season
Good, cause of disease is few, and silkworm silk yield is high and stably.Heretofore described Nature enemy is preferably to drop silkworm to Sang Zhiliang
For original 70~90%, more preferably 80%；Described Nature enemy can also be to be placed on small straw bundles to spin cocoons for 1~2 day in advance, such as in certain kind
Under standard atmosphere grown silkworm 5 age silkworm should it is aging at the 7th day on cluster cocoon, Nature enemy is allowed on grown silkworm at the 5th day or the 6th day
Cluster, the grown silkworm being placed on small straw bundles to spin cocoons in advance started spinning and cocoond 8~9 days 5 ages.The purpose of Nature enemy of the present invention is to pass through famine
Starving processing influences cultivated silkworm antimicrobial peptide, silk gland cell cycle associated genes mRNA etc. expression, so as to influence the spinning amount of silkworm and
Resistance.The present invention preferably selected after Nature enemy cocoon type just, partially thin, cocoon weight, cocoon shell weight, cocoon layer in median size, contracting wrinkle
The rate male and female individual close with original vector kind 881,882 is used as target individual, establishes multiple alternative strains.The target individual
Preferable mark for：881R0601,881R0602,881R0603,881R0604 ..., 882R0601,882R0602,
882R0603、882R0604……。
The present invention ties up to autumn after multiple alternative strains are obtained, by multiple choices of acquisition and carries out raising identification, selects
The silkworm strain for selecting multifibres amount and anti-nuclear polyhedrosis is oriented cultivation, obtains multiple orientation strains.Institute in the present invention
State raising to be accredited as after silkworm breeding process assembles the stage into hybridization, take and raising sight is carried out to multiple alternative strain groups
Examine, investigation records Yield and quality and Resistance index.The present invention selects disease resistance under conditions of high temperature and the food value of leaf are poor in the fall
By force, the orientation strain of multifibres amount；Target variety can fast and accurately be obtained.Heretofore described post-directed training, refer to be directed to
Some specific character constantly carries out Secondary Culture, constantly strengthens specific trait.Such as selected from multiple alternative strains more
This characteristic that the strain of silk amount, constantly culture strengthen multifibres amount obtains the strain that multifibres amount character significantly improves.
The present invention carries out day after multiple orientation strains are obtained, to multiple orientation strains of acquisition × in, everyday ×
In in, day × in, everyday × middle form combines cross combination, is hybridized；By identifying the anti-of filial generation
Characteristic of disease, detection silk amount, selection obtain the strong cultivated silkworm breed variety of multifibres amount, anti-nuclear polyhedrosis.The present invention is preferred in after hybridization
Select multifibres amount, the cultivated silkworm breed variety that anti-nuclear polyhedrosis is strong and coordinate force is good.Heretofore described coordinate force is two miscellaneous
With respect to the ability combined between friendship parent.The present invention is preferable in the selection cross of polynary kind both to have test homology original in polynary kind
The coordinate force of kind, while also to test coordinate force of the polynary kind to friendship original seed.The test of heretofore described coordinate force is using this
The conventional coordinate force testing method in field, without other particular/special requirements.
The present invention is after the strong cultivated silkworm breed variety of multifibres amount, anti-nuclear polyhedrosis is obtained, preferably to the cultivated silkworm breed variety of acquisition
Field trial, variety and quality identification, variety certification or registration are carried out, is bred as target variety.The field trial in the present invention
Supported for the cultivated silkworm breed variety of acquisition to be issued to peasant household and carry out examination with compareing cultivated silkworm breed variety, and determine following parameter：Big silkworm rearing season development
By, dead cage rate, cocoon weight, cocoon shell weight, Cocoon layer ratio, one-level cocoon rate, kind of the amount of cocoon production and kilogram cocoon grain number.The field trial
Purpose be to examine whether the various aspects of performance of cultivated silkworm breed variety obtained is stablized, the inspection of actual production can be withstood.This
The above-mentioned character of cultivated silkworm breed variety described in invention should be better than control cultivated silkworm breed variety or at least identical with control cultivated silkworm breed variety.The present invention
Described in variety and quality identification, variety certification or the program for being registered as the conventional determination silkworm novel species in this area, it is special without other
It is different to require.
With reference to specific embodiment to multifibres amount of the present invention, the breeding side of anti-nuclear polyhedrosis (NPV) silkworm
Method is further described in detail, and technical scheme includes but is not limited to following examples.
Embodiment 1
It is purebred 881 hybridization with the basic material RB of the anti-nuclear polyhedrosis gene of carrying, obtains (881 × RB)
F1881×RBCenospecies；With the basic material RB and Japanese purebred 882 hybridization for carrying anti-nuclear polyhedrosis gene, obtain (882 × RB)
F1882×RBCenospecies；3) (881 × RB) F is used1881×RBCenospecies and (882 × RB) F1882×RBCenospecies is respectively with carrying anti-blood
The basic material RB continuous backcrosses of type pus illness gene 16 times, obtain the kind to nuclear polyhedrosis resistance, are designated as 881RB respectively
And 882RB；4) Nature enemy is carried out after spring silkworm rearing season raising 881RB and 882RB to the 5th day 5 ages, grown silkworm 5 age silkworm is in standard temperature
Under humidity should it is aging at the 7th day on cluster cocoon, but allowed at the 5th day or the 6th day thereon (it is anti-in addition to cover cluster net to cluster for capping on cluster
Jump), treat 8~9 days, be forced the silkworm egg of upper cluster and can also start spinning to cocoon；Establish multiple alternative strains, select cocoon type just, it is medium
Partially thin in size, contracting wrinkle, cocoon weight, cocoon shell weight, Cocoon layer ratio and the close male and female individual of original vector kind 881,882 are used as target
Individual, it is labeled as：881R0601,881R0602,881R0603,881R0604 ... ... ..., 882R0601,882R0602,
882R0603,882R0604 ... ... ...,；The multiple choices obtained are tied up into autumn and carry out raising identification, select multifibres
Measure and the silkworm strain of anti-nuclear polyhedrosis is oriented cultivation, obtain multiple orientation strains；Multiple orientation strains are carried out
Day × in, everyday × in, day × in, everyday × middle form combines cross combination, is hybridized；Pass through mirror
Determine disease resistance, the detection silk amount of filial generation, selection obtains the strong cultivated silkworm breed variety of multifibres amount, anti-nuclear polyhedrosis, is
881R0601881R0603 (being original seed in your silkworm No. 16), 882R0601882R0603 (No. 16 Japanese original seeds of your silkworm).
The new varieties original silkworm behavior study that method obtains in the embodiment 1 of embodiment 2.
Check variety：Cyanines pine (being original seed in Jingsong×Haoyue), bright moon (the Japanese original seed of Jingsong×Haoyue), new varieties：
881R0601881R0603 (being original seed in your silkworm No. 16), 882R0601882R0603 (No. 16 Japanese original seeds of your silkworm) are pair
According to.
Autumn silkworm rearing season in 2016, after varietal character is basicly stable, in order to grasp new varieties cross combination two to handing over original seed
Character, the China to the kind is original seed 881R0601881R0603, and Japanese original seed 882R0601882R0603 is raised
Educate, investigation is tested to 10 moth egg amounts, dead ovum amount, nonfertilization ovum amount etc., check variety is cyanines loose (middle system), bright moon (day
System), 881R0601881R0603 (middle system), 882R0601882R0603 (Japanese) raise and investigate in the same way.
Original silkworm rearing
The original silkworm egg outbound time hastens the hatching of silkworms with the outbound simultaneously such as identification kind and product guarantor, and standard of hastening the hatching of silkworms is entered by the gradual standard of hastening the hatching of silkworms
OK.Each kind is collected ants 0.5g, is educated with common four times after method is raised to four-aged silkworm and is determined silkworm number.Each kind sets two repetitions
Cell, each 400 silkworms of cell, raises to aging and cocoons, and two a small bundle of straw, etc. for silkworms to spin cocoons ons in every small differentiation when being placed on small straw bundles to spin cocoons, per a small bundle of straw, etc. for silkworms to spin cocoons on, a small bundle of straw, etc. for silkworms to spin cocoons on net, 6 sufficient days are covered in capping
Harvesting of cocoon, peel every achievement such as husks, 7 sufficient day investigation cocoon qualities, cocoon shape, cocoon color off.Cut open cocoon mirror pupa after the moth appearance production of hybrid seeds, to hand over kind with
Machine post-coitum list moth egg, identified as Cocoon quantity and quality achievement of future generation, while investigate single moth egg amount and good ovum rate.Simultaneously
The normal female moth of choosing emergence is mated with to friendship kind after 4h, and 10 moths of random selection connect laying eggs on paper in common species, and four are framed with wooden frame
Side, and pay attention to patrolling moth, do not allow it that silkworm seed is originated on wooden frame side or connected outside paper, China is that female moth is respectively repeated 4 times with Japanese female moth.Adjust
Look into the achievements such as total egg laying amount, nonfertilization ovum, dead ovum.
The production of hybrid seeds is tested
Collect ants 2 grams per kind, educated feeding as common and cocoond, 3d of cocooing adopts immature cocoon and is laid in bamboo tray for raising silkworms, adjusts at seven sufficient days
Breeding achievement is looked into, selects same palace and light defective cocoon, cuts open cocoon mirror pupa, the moth appearance production of hybrid seeds, every company's paper throws moth of moth 120 or so, pays attention to patrolling moth, produces
Man Houhuan moves even paper.
Select moth body colour
In catching moth, moth is thrown in mating, intending throwing moth overall process to, the production of hybrid seeds to, folding, 881R0601881R0603 routinely product
Kind selection；882R0601882R0603 selects moth color canescence, and the silkworm moth that moth wing is sufficiently spread out, palea is neat is production of hybrid seeds moth,
Pottery eliminates non-black moth and bad moth.
Above-mentioned every spawning character experiment, by 80% humidity, 25 DEG C of temperature of laying eggs, humidity 75% or so, Mingguang City's mating,
Dark protection spawning.
Interpretation of result
Original silkworm rearing developmental duration and moth appearance time
Original silkworm rearing developmental duration is shown in Table 1 with the moth appearance time, and as seen from Table 1, new varieties original silkworm, its age passes through compared with cyanines
Pine, bright moon are short, suitable with 881R0601881R0603,882R0601882R0603.The moth appearance time is in the morning 6 up to 9
When or so, the moth appearance time is concentrated in the morning 7：30 or so, this is advantageous to the conventional method production of hybrid seeds, saves the time and is thrown with labour
Enter.
No. 16 original silkworm rearings of your silkworm of table 1 and moth appearance list of results
Original silkworm rearing health and production of hybrid seeds achievement
In terms of health, as seen from Table 2, No. 16 China of your silkworm are that kind 881R0601881R0603 worm pupa rates reach
99.89%, than cyanines pine 1.00 percentage point high 1.73 percentage points, higher than 881R0601881R0603；Japanese kind
882R0601882R0603 worm pupas rate is 99.93%, higher than bright moon 2.04 percentage points, higher than 882R0601882R0603
1.80 percentage point.General cocoon rate is also higher, and the 881R0601881R0603 of system of China general cocoon rate is smaller than cyanines pine up to 98.59%
It is 0.18 percentage point, smaller than 8,81R,060,188,1R0,603 0.39 percentage point；Japanese 882R0601882R0603 reaches
98.70%, it is higher than bright moon 1.02 percentage points, it is smaller than 8,82R,060,188,2R0,603 0.03 percentage point.In terms of cocoon quality, system of China
881R0601881R0603 cocoon weight is higher than cyanines pine respectively up to 1.68g, cocoon shell weight up to 0.40g, Cocoon layer ratio 23.81%
0.03g, 0.0g and small 0.14 percentage point, the complete phase of cocoon weight, cocoon shell weight, Cocoon layer ratio with 881R0601881R0603
Deng；Japanese 882R0601882R0603 cocoon weight 1.69g, cocoon shell weight 0.39g, Cocoon layer ratio 23.67% are higher than bright moon respectively
0.02g, 0.0g and small 0.21 percentage point, respectively 0.28 percentage higher 0.02g, 0.0g than 882R0601882R0603 and small
Point.
Moth color selection result, the black moth rates of 882R0601882R0603 are high, and moth body is normal, and health is good.
No. 16 kind production of hybrid seeds list of results of your silkworm of table 2
As can also be seen from Table 2, your silkworm No. 16 and cyanines pine, bright moon and 881R0601881R0603,882R0601
882R0603 ratios, the cyanines pine of kilogram system of cocoon production of hybrid seeds amount China is 3.81, and 881R0601881R0603 is 4.1,
881R0601881R0603 is 3.74, and new varieties achievement compares kind close to two；Japanese bright moon is 3.65,
882R0601882R0603 is 4.2, and 882R0601882R0603 is 3.83, and new varieties achievement is higher than bright moon, is approached
882R0601·882R0603.The cyanines pine of system of gram ant production of hybrid seeds China is 15.5, and 881R0601881R0603 is 16.0,
881R0601881R0603 is 15.5, and new varieties production of hybrid seeds achievement compares kind close to two；Japanese bright moon is 15.25,
882R0601882R0603 is 16.5, and 882R0601882R0603 is 15.7, it is seen that new varieties are higher than bright moon, approach
882R0601·882R0603。
In order to further analyze the significance of difference of achievement between each kind, to the major traits worm pupa of resistance in feeding
Rate, most representational cocoon weight carries out variance analysis (table summary) and Multiple range test in amount of cocoon production major traits amount of cocoon crop and cocoon quality
In table 3, table 4.As can be seen from Table 3, the size order of worm pupa rate is 882R0601882R0603 ＞ 881R0601
881R0603 ＞ 881R0601881R0603 ＞ cyanines pine ＞ 882R0601882R0603 ＞ bright moons, Combination nova original seed
Pole significant difference (P ＜ 0.01) with cyanines pine be present in 881R0601881R0603,882R0601882R0603.Former silkworm whole cocoon
Amount, which compares, is shown in Table 4, and size order is 882R0601882R0603 ＞ 881R0601881R0603 ＞ 881R0601
881R0603 ＞ 882R0601882R0603 ＞ bright moon ＞ cyanines pine, 2 original seed cocoon weights of Combination nova have differences with bright moon
But not significantly (P ＜ 0.05).
It can be seen that No. 16 kind original silkworm characters of your silkworm are relatively stable, each character is near or above check variety, up to practical water
It is flat.
Table 3 original seed 881R0601881R0603,882R0601882R0603 raise worm pupa rate Multiple range test
Note：* represents significant difference in table, and * * represent pole significant difference, similarly hereinafter.
Original seed 881R0601881R0603,882R0601882R0603 the cocoon weight Multiple range test of table 4
Original silkworm spawning achievement
10 moth egg amounts and archiblast investigation result are shown in Table 5, it can be seen that 881R0601881R0603 egg laying amount average out tos
486, good ovum rate is up to 97.03%；The total egg laying amounts of 882R0601882R0603 are up to 445, good ovum rate 97.51%.It can be seen that nothing
By being system of China or Japanese original seed, egg laying amount is more, and 10 moth egg amounts are average, shows that varietal character is relatively stable, this and conventional product
Kind spawning quantity research correlated results coincide.
Original seed 881R0601881R0603,882R0601882R0603 mother moth egg of table 5 is investigated
Silkworm commercial variety breeding objective requires that silkworm egg is easily numerous, i.e., the resistance of former silkworm is stronger, and general cocoon rate is high, gram ant
Production of hybrid seeds amount is just high.Cocoon quality requires excellent, is not only directly connected to the height of production of hybrid seeds amount, also influences the silk of filial generation (general kind)
Quality, particularly Cocoon layer ratio are high, and just there will be higher Cocoon layer ratio in lower generation.
Find out from the interpretation of result of the above-mentioned rearing of parent silkworm, No. 16 kind original seed 881R0601881R0603 of your silkworm,
882R0601882R0603 shows better resistance, and general cocoon rate is higher, cocoon quality preferably feature, therefore not only can reach silkworm egg
Yi Fan requirement, it is preferably horizontal that lower generation achievement also can reach Comprehensive Traits.
The expensive silkworm of new varieties with disease-resistant gene No. 16 (881R0601881R0603,882R0601882R0603), its
Rearing of parent silkworm performance is development is neat, health is good, resistance is relatively strong, the amount of cocoon production is higher, cocoon quality is more excellent.Moth appearance is more early,
Moth appearance is neat, and 882R0601882R0603 male and female moth color shows canescence, and moth color is consistent；Female moth mates with to friendship kind
Afterwards, its spawning is more early, relatively concentrates, egg laying amount is more, and archiblast is excellent.Compared with production of hybrid seeds experiment being carried out with check variety reciprocal cross, its
Gram ant production of hybrid seeds amount is close to control kind.Comprehensive every achievement can show that your silkworm No. 16 is that a pair of comprehensive characters are excellent to handing over original seed, is removed
Outside band black moth target character, its original seed character maintain the expensive silkworm of original variety No. 16 parents (881R0601881R0603,
Excellent production performance 882R0601882R0603), egg laying amount is more, production it is attached it is good, ovum color is consistent, be a pair with disease-resistant gene
Practical multiple cross new varieties.
The field trial experiment for the new varieties that method obtains in the embodiment 1 of embodiment 3.
Field trial is tested
1.1 material to be tested
It is the expensive silkworm of Combination nova No. 16 (area's examination numbering) to be filtered out from the disease-resistant material identified for examination Silkworm varieties, compares silkworm product
Kind Guangdong and Guangxi Provinces 2.
1.2 test method
Unify outbound for examination Silkworm varieties and check variety, educate altogether with being unified in each demonstration and a little hasten the hatching of silkworms, collect ants and the cooperative rearing of young silkworm.
Experiment kind and control kind are educated to 3 age first feedings per paper tinsel 2g ant amounts and are distributed to each experiment family one day after altogether.Test family selection technique water
Flat, plant mulberry tree breed is consistent, plant time consistency, and 3 relatively uniform family peasant households of facilities and equipment are carried out.Rearing of grown silkworm is respectively tested
Identical technical finesse is taken in family as far as possible, and full age educates raising using daily 3 times.In order to compare raising data accuracy, in silkworm
Sheng adopts a small bundle of straw, etc. for silkworms to spin cocoons on the 6th day after being placed on small straw bundles to spin cocoons, the 7th day from concentrate harvesting of cocoon it is a collection of in randomly select 3 samples, each 3 kilograms of sample, by 9 kilograms of silkworms
Cocoon therefrom extracts 3 samples again after being sufficiently mixed, and each 1 kilogram of silk cocoon of sample is investigated sample cocoon.Full period survey item：Big silkworm rearing season development
By, dead cage rate, cocoon weight, cocoon shell weight, Cocoon layer ratio, one-level cocoon rate (one-level cocoon determines standard by corporate buyout's standard), kind
The amount of cocoon production, kilogram cocoon grain number.It is identical with experiment kind to compare the investigation of Silkworm varieties items achievement.
2.2.1 result and analysis
2.2.1 field trial achievement
2.2.1.1 prestige Ning County gold bushel town Hei Ni villages
From table 6, No. 16 spring of your silkworm, Guizhou height above sea level was most in Guizhou height above sea level highest county prestige Ning County (height above sea level 2200m)
High sericulture area prestige Ning County gold bushel town (height above sea level 1700m) rural area raising, dead cage rate reduce by 10.17 percentage points than control, whole cocoon
1.91g, cocoon shell weight 0.44g, Cocoon layer ratio 23.17% are measured, improves 11.05%, 15.99% and 0.93 percentage than control respectively
Point；One-level cocoon rate 98.50%, 1.18 percentage points are improved than control；Average kind of amount of cocoon production 42.47kg, is improved than control
11.04%.Kind of 1756.71 yuan of an output value is opened, relatively control increases by 13.12%.
Table 6 your silkworm No. 16 is compared with the raising of control No. 2 Guizhou, Guangdong and Guangxi Provinces Bu Can regions
(spring silkworm rearing season average achievement-Wei Ning in 2015,2016), height above sea level：1700m
From table 7, No. 16 summers of your silkworm raise in prestige Ning County gold bushel town rural area, and dead cage rate reduces by 9.77 percentages than control
Point, cocoon weight 1.90g, cocoon shell weight 0.43g, Cocoon layer ratio 22.66%, improve 10.16%, 11.69% and 0.31 than control respectively
Percentage point；One-level cocoon rate 97.22%, 0.91 percentage point is improved than control；Average kind of amount of cocoon production 42.15kg, than control
Improve 10.48%.Kind of 1741.66 yuan of an output value is opened, relatively control increases by 11.54%.
Table 7 your silkworm No. 16 is compared with the raising of control No. 2 Guizhou, Guangdong and Guangxi Provinces Bu Can regions
(the silkworm rearing season average achievement-Wei Ning of summer in 2015,2016), height above sea level：1700m
In terms of table 6, the achievement of table 7, in High aititude silkworm area, the adaptability your silkworm is No. 16 is still fine, and yield is high, cocoon quality achievement
It is excellent, show high yield characteristic.In more than 1700 meters of silkworm area of height above sea level, your silkworm No. 16 has good adaptability, shows rich
Produce performance.Few that pus illness occurs, develops and supports neatly, well, aging upper cluster is neat.Cocoon quality achievement is excellent, kind of an amount of cocoon production, kind of a production
Value substantially improves than check variety.And it is cocoon quality in each trial zone, yield highest silkworm area.Illustrate the kind in plateau silkworm
Area, it is that an adaptability is good to raise your silkworm No. 16, is got bumper crops and the Silkworm varieties of health.
Raise disease-resistant kind technical essential in plateau silkworm area：1. spring silkworm rearing season is focused on being incubated at night, day and night temperature is reduced；2. silkworm
Before the strict silkworm in family, in silkworm, environment disinfected after silkworm, focus on disinfection of tools for silkworm rearing.3. strictly wash one's hands to mulberry, change footwear and enter the room system, strict silkworm
Has subregion land use reform.
2.2.1.2 Huangping County
From table 2, No. 16 spring of your silkworm raise in the silkworm area of Guizhou height above sea level 640 or so, and dead cage rate reduces than control
11.82%, cocoon weight 1.90g, cocoon shell weight 0.43g, Cocoon layer ratio 22.79%, improve 13.96%, 9.31% and than control respectively
1.02 percentage point；One-level cocoon rate 97.13%, 1.04 percentage points are improved than control；Average kind of amount of cocoon production 41.54kg, than
Control improves 15.20%.Kind of 1694.71 yuan of an output value is opened, relatively control increases by 16.44%.
Table 8 your silkworm No. 16 is compared with the raising of control No. 2 Guizhou, Guangdong and Guangxi Provinces Bu Can regions
(spring silkworm rearing season average achievement-Huangping in 2015,2016), height above sea level：640m
From table 8, No. 16 summer silkworm rearing seasons of your silkworm are raised in Huangping County rural area, and dead cage rate reduces by 12.32 percentages than control,
Cocoon weight 1.88g, cocoon shell weight 0.43g, Cocoon layer ratio 22.93%, improve 12.61%, 18.62% and 1.16 hundred than control respectively
Branch；One-level cocoon rate 96.39%, 0.30 percentage point is improved than control；Average kind of amount of cocoon production 40.73kg, is improved than control
12.96%.Kind of 1668.97 yuan of an output value is opened, relatively control increases by 13.31%.
Table 9 your silkworm No. 16 compared with the raising of control No. 2 Guizhou, Guangdong and Guangxi Provinces Bu Can regions (summer silkworm rearing season in 2015,2016 it is average into
Achievement-Huangping), height above sea level：640m
In terms of table 8, the achievement of table 9, in Guizhou southeast of Guizhou Province Middle altitude silkworm area, No. 16 yield height of your silkworm, cocoon quality achievement are excellent,
Show high yield characteristic.Few that pus illness occurs, develops and supports neatly, well, aging upper cluster is neat.Cocoon quality achievement is excellent, kind of a production cocoon
Amount, kind of an output value substantially improve than check variety.Technical essential：Reasonable employment mascardine-proof dust, sterilized before strict silkworm, in silkworm, after silkworm,
It is required that relevant departments of county coordinate agriculture and animal husbandry bureau of county to coordinate the Committee of Development and Reform of county, Deng Duojia departments of agriculture and animal husbandry bureau of county, paid service is carried out.
2.2.1.3 Libo County Mao Lan towns
From table 10, No. 16 spring of your silkworm raise in the rural area such as the Libo County Mao Lan towns Yao Ai villages of low altitude area and Yao Ming villages.
Dead cage rate reduces by 7.43 percentage points than control, cocoon weight 1.84g, cocoon shell weight 0.43g, Cocoon layer ratio 23.20%, respectively than control
Improve 12.35%, 22.85%, 2.01 percentage point；One-level cocoon rate 98.20%, 1.39 percentage points are improved than control；It is average
Kind of an amount of cocoon production 40.78kg is opened, 13.97% is improved than control.Kind of 1701.97 yuan of an output value is opened, relatively control improves 15.62.
Table 10 your silkworm No. 16 is compared with the raising of control No. 2 Guizhou, Guangdong and Guangxi Provinces Bu Can regions
(spring silkworm rearing season average achievement-Libo in 2015,2016), height above sea level：425m
From table 11, No. 16 summers of your silkworm raise in the rural area such as Libo Mao Lanzhen Yao Ai villages and Yao Ming villages, and dead cage rate compares
According to reducing by 11.22 percentage points, cocoon weight 1.87g, cocoon shell weight 0.43g, Cocoon layer ratio 23.03%, improved respectively than control
13.33%th, 19.44% and 1.18 percentage points；One-level cocoon rate 96.39%, 0.30 percentage point is improved than control；Average kind
Amount of cocoon production 40.57kg, 13.74% is improved than control.Kind of 1662.47 yuan of an output value is opened, relatively control increases by 14.06%.
Table 11 your silkworm No. 16 is compared with the raising of control No. 2 Guizhou, Guangdong and Guangxi Provinces Bu Can regions
(summer silkworm rearing season average achievement-Libo in 2015,2016), height above sea level：425m
In terms of table 10, the achievement of table 11, in Guizhou low altitude area silkworm area, the silkworm area of 400 meters or so of height above sea level, your silkworm No. 16 also have
Good adaptability, high yield characteristic is shown, yield is high, cocoon quality achievement is excellent.Few that pus illness, stiff disease etc. occurs, development is neat
Spend, be good foster, aging upper cluster is neat.Cocoon quality achievement is excellent, and kind of an amount of cocoon production, kind of an output value substantially improve than check variety.Say
For the bright kind in Guizhou low altitude area silkworm area, it is that an adaptability is good to raise your silkworm No. 16, the Silkworm varieties of high yield and health.
Applied in low altitude area silkworm area, technical essential is fully to make good use of mascardine-proof dust, meets the day rainy day to silkworm body and seat with once
Mascardine-proof dust.It is ventilated to focus on cocoonery, strict each link sterilization.
From above-described embodiment, the expensive silkworm of new silkworm variety No. 16 that breeding method of mulberry silkworm of the present invention obtains is expensive
State 1700m height above sea level, 600m height above sea level, 400m height above sea level silkworm areas are compared analysis for No. two with Guangdong and Guangxi Provinces for control, the results showed that, your silkworm 16
Number in spring, Xia Liangjige silkworms area without obviously difference.Then seldom develop the silkworms such as pus illness, stiff disease in spring and summer in terms of dead cage rate
Disease, the incidence of disease are substantially less than check variety, strong adaptability.Each silkworm area raising, No. 16 yield height of your silkworm, development are neat, and cocoon quality is excellent
Good, achievement is suitable for the large scale application of Guizhou Different Altitude silkworm area better than control kind.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of multifibres amount, the breeding method of anti-nuclear polyhedrosis silkworm, comprise the following steps：
1) it is purebred 881 hybridization with the basic material RB of the anti-nuclear polyhedrosis gene of carrying, obtains (881 × RB) F1881×RB
Cenospecies；
2) with the basic material RB and Japanese purebred 882 hybridization for carrying anti-nuclear polyhedrosis gene, (882 × RB) F is obtained1882×RBIt is miscellaneous
Hand over kind；
3) (881 × RB) F obtained with the step 1)1881×RB(882 × RB) F that cenospecies and step 2) obtain1882×RBIt is miscellaneous
Hand over kind respectively with carry anti-nuclear polyhedrosis gene basic material RB continuous backcrosses several times, obtain to nuclear polyhedrosis resistance
Strong kind, is designated as 881RB and 882RB respectively；
4) 881RB and 882RB obtained in the spring silkworm rearing season raising step 3), hungry place is carried out after raising to the 5th day 5 ages
Reason, establishes multiple alternative strains；
5) the multiple choices for being obtained step 4) tie up to autumn and carry out raising identification, select multifibres amount and anti-blood type purulence
The silkworm strain of disease is oriented cultivation, obtains multiple orientation strains；
6) the multiple orientation strain days obtained to step 5) × in, everyday × in, day × in, everyday × middle group
Cross combination is made into, is hybridized；By identifying disease resistance, the detection silk amount of filial generation, selection obtains multifibres amount, anti-blood
The strong cultivated silkworm breed variety of type pus illness；Wherein described " in " it is Chinese system kind, " day " is Japanese system kind, " " and "×" table
Show hybridization；
Limited between the step 1) and step 2) without time sequencing.
2. breeding method according to claim 1, it is characterised in that the number of the continuous backcross described in step 3) is 4
~20 times.
3. breeding method according to claim 1, it is characterised in that the Nature enemy described in step 4) is to give silkworm
Sang Zhiliang is reduced to the kind should be to the 70~90% of Sang Zhiliang.
4. breeding method according to claim 1, it is characterised in that the Nature enemy described in step 4) is in advance 1~2
It is placed on small straw bundles to spin cocoons.
5. breeding method according to claim 1, it is characterised in that the cross combination described in step 6) includes 881RBN
×881RBN、881RBN×882RB1·882RBN、881RBN·881RBN×882RB1、881RBN·881RBN×882RB1·
882RBN。
6. breeding method according to claim 1, it is characterised in that it is strong that step 6) obtains multifibres amount, anti-nuclear polyhedrosis
Cultivated silkworm breed variety after, in addition to field trial, variety and quality identification, variety certification or registration are carried out to the cultivated silkworm breed variety of acquisition,
It is bred as target variety.
7. breeding method according to claim 6, it is characterised in that the field trial is the silkworm for obtaining step 6)
Kind is issued to peasant household and carries out examination with compareing cultivated silkworm breed variety and supports, and determines following parameter：Big silkworm rearing season developmental duration, dead cage rate, entirely
Cocoon amount, cocoon shell weight, Cocoon layer ratio, one-level cocoon rate, kind of the amount of cocoon production and kilogram cocoon grain number.
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| CN114698604A (en) * | 2022-04-07 | 2022-07-05 | 辽宁省蚕业科学研究所 | Breeding method for resisting hunger and nutrition stress of tussah larva |
| CN116671499A (en) * | 2023-06-28 | 2023-09-01 | 吉林省蚕业科学研究院(吉林省农特产品研究院、吉林省北冬虫夏草研究中心) | Tussah comprehensive hybrid seed creation method |
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