CN102217531A - Breeding method for tropical high-oil maize self-bred line - Google Patents
Breeding method for tropical high-oil maize self-bred line Download PDFInfo
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Abstract
The invention discloses a breeding method for a tropical high-oil maize self-bred line, and belongs to the technical field of crop breeding. In the method, temperate high-oil maize hybrids is used as a donor parent, a tropical maize self-bred line is used as a recurrent parent, one-generation cross breeding, two-generation backcross and five-generation selfing are performed, in backcross and selfing, the tropical maize self-bred line is used as a male parent in backcross and selfing, large-germ seeds are selected in each generation in backcrossing and selfing, namely seeds of which seed germ planes account for more than 3/4 of seed planes are selected, and the tropical high-oil maize self-bred line is bred. The tropical high-oil maize self-bred line bred by the method integrates the high oil content of temperate high-oil maize germplasm and the high resistance of tropical maize germplasm, is high in expression quality, resistance and yield, and particularly is obviously superior to the original temperate high-oil maize in aspects of stress resistance and yield, so the basis of the temperate high-oil maize germplasm is broadened, and the excellent basis is established for the breeding of high-oil maize hybrids in tropical and subtropical zones.
Description
Technical field
The invention belongs to the crop breeding technical field, be specifically related to a kind of selection of tropical high oil corn self interweaving series.
Background technology
High oil corn is meant in mass fraction, its grain oil content reaches more than 6% (promptly in mass fraction, represent its grain oil content with full seed content of crude fat), other proterties a kind of novel high-grade maize similar to conventional corn, and the conventional corn oil content only is 3-4%.High oil corn has not only improved the total energy level of corn kernel greatly, has also improved its multiple nutritional components comprehensively, is the important source of high energy high-quality feed.Feeding experiment shows both at home and abroad, raises pigs with high oil corn, improves more than 20% than conventional corn feed daily gain, reduces feed 14%; The daily gain of raising chickens can improve 8-10%, and does not need extra interpolation fat, thereby has reduced feed cost.
High oil corn research originating in the earliest American I llinois university in 1896, it is an important topic of maize genetic breeding subject, but there is the narrow significant deficiency in blastogenesis basis in high oil corn, the high oil corn breeding all is to utilize IHO basically in the world at present, three temperate zone high oil corn colonies of Iowa and Alexho, particularly (DuPont) company of Du Pont of U.S. trans-corporation and the joint purchase of Pfister seeds company comprise the exclusive right to use of whole high oil corn germplasms of ALEXHO and IHO, high oil corn research mainly concentrates on a few mechanism, has seriously limited the development of high oil corn.China begins high oil corn research the eighties in 20th century, through constantly improvement and innovation for many years, create a plurality of high oil corn colonies, and utilize these germplasms to select more than 10 oil content at the high-oil corn variety more than 10% with complete independent intellectual property rights.But because the employed temperate zone germplasm that mostly is of China, the high-oil corn variety of breeding shows generally in China torrid zone, subtropical zone that disease resistance is relatively poor, output is lower, is difficult in China torrid zone, apply the subtropical zone.The torrid zone, subtropics corn germplasm because exchanges less with the temperate species plasmagene for a long time, hereditary difference is big, is improvement, the important germ plasm resource of the temperate zone corn that increases.Therefore, the seed selection of the torrid zone, subtropics high oil corn germplasm, utilization are the developing direction of high oil corn breeding research from now on.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the defective that prior art seed selection high oil corn blastogenesis basis is narrow, lack the high oil corn germplasm that adapts to the torrid zone, subtropical zone, its objective is the selection that a kind of tropical high oil corn self interweaving series is provided.
For solving the problems of the technologies described above, technical scheme of the present invention is as follows:
A kind of selection of tropical high oil corn self interweaving series is to be that donor parents, tropical corn inbred line are recurrent parent with temperate zone high oil corn crossbreed, by 1 generation hybridization, in 2 generations, backcrossed and the selfing of 5 generations, hybridizing and backcrossing is male parent with tropical corn inbred line all, backcross simultaneously and selfing per generation is selected big embryo seed, seed selection torrid zone high oil corn self interweaving series, described big embryo seed is that the embryo plane accounts for the seed of seed plane more than 3/4.
Described temperate zone high oil corn crossbreed is high oil 115, and described tropical corn inbred line is CML171.
The tropical high oil corn self interweaving series of institute's seed selection is CML171-1-1-2-1-1.
The high oil 115 of biomaterial involved in the present invention, CML171 and CML171-1-1-2-1-1 are all open in non-patent literature, and above-mentioned biomaterial application has preservation per capita, and the applicant guarantees to provide to the public in 20 years present patent application days.Applicant contact address: Kunming, Yunnan Province Panlong District tap street peach garden village Cereal Crops Inst., Yunnan Prov. Agricultural Academy, telephone number: 0871-5892503, postcode: 650205.
High oily 115 at Song Tongming. the high oily 115. crop magazines of 1997. corn hybrid seed agricultural universities, 4:38., Chen Xizhou, Wang Li, Fan Yuliang, Cui Qin, Zhang Fengying, Cai Yukuan. the introduction of 1998. high oily 115 corns and exploitation preliminary study. the corn science, 6(1): 58-60., Jiang Zhiliang, Wang Lijie, Zhou Qingwu. the high oily 115 corn hybrid seed introduction and Experiment of 1999. agricultural universities. external agronomy-coarse cereals crop, 19 (1): 38-39., the kingdom peak, Xing Haizhong, Zhang Xiufen, Sun Jiuxin, in richness. 2001. high oil 115 are introduced research and plantation. the corn science, 9 (1): 54-56., Gao Shibin, Li Wanchen. 2001. high-oil corn varieties " high oil 115 " are in the compatibility test and the production demonstration in Sichuan Province. the corn science, 9 (4): 65-.66, Lu Minghua, cover just refined, Deng Chengde. 2003. high oil " 115 " and local corn silage material are to the comparative test of cow producing milk performance. China's Dairy Industry, 11:20-21., Zhao Zixian, Gao Xiangkuo, Wang Yunmei, Sun Jiarong, Yankee is prosperous, Kong Jianhua, Fan Zhenghua, the text of an annotated book is farsighted. the research and utilization of 2004. Yunnan high oil corns and development countermeasure. and Yunnan Prov Agriculture University's journal, 19(6): 685-691., Song Huixin, Zhao Yongzhi, Zhou Chunjiang, Yang Peizhu, Hou Fuqiang is permitted Yongxin. 2004. high oily 115 growth regularities and corresponding cultivation techniques research. and the corn science, the 12(monograph): 84-87., Wu Jianming, beam and, Tang Shiyun, Lu Guoying. 2005. Different Fertilization levels and density are to the influence of oily 115 corn yields of height and quality. the Guangxi agricultural science, 36(2): 138-140., Gan Fengqiong, Zhang Yaping, Wu Zikai. the dry-matter accumulation rule research of 2008. little endosperm super-high oil corns and high oily 115 corns. seed, 27(6): open among the 5-7..
CML171 is at a kind Xing Ming, Tan Jing, Huang Bihua, Liu Feng. 2001. Analysis of combining ability and heterotic groups of yellow grain quality protein maize inbreeds. heredity, 23(6): 547-552., kind Xing Ming, Zhang Shihuang, Tan Jing, Li Mingshun, Li Xinhai. 2003. hybrid vigour groups according to SSR mark division high-quality protein maize inbred line. Acta Agronomica Sinica, 29(1): 105-110., Fan, X.M., J. Tan, J.Y. Yang, and H.M. Chen. 2004. Combining ability and heterotic grouping of ten temperate subtropical and tropical quality protein maize inbreds. MAYDICA, 49:267-272., kind Xing Ming, Chen Hongmei, Tan Jing, Yang Junyun, yellow sky, section Chile. 2005. high-quality protein maize inbred line Other Main Agronomic Characters Analysis of Combining Ability. Acta Agronomica Sinica, 31(5): 540-544., kind Xing Ming, Chen Hongmei, Tan Jing, Liu Feng, Han Xuerui, yellow sky, Duan Zhili. 2006. tropical and subtropical zone high-quality protein maize inbred lines and temperate zone conventional corn inbred line output COMBINING ABILITY. corn science, 14 (1): 12-15,32., Wu Jiancong, Xu Chunxia, Chen Hongmei, Tan Jing, Han Xuerui, Huang Bihua, kind emerging bright. 2007. 24 high-quality protein maize inbred lines and Chinese temperate zone corn four big advantage groups represent the coordinate force and the hybrid vigour group research of inbred line. Scientia Agricultura Sinica, 40 (6): 1288-1296., Sun Chengtao, Chen Hongmei, Tan Jing, Xu Chunxia, Li Xuezhi, Huang Bihua, kind emerging bright. the COMBINING ABILITY of 2007. 25 good temperate zone corn inbred lines and 4 tropical corn inbred line economical characters. the corn science, 15 (3): 36-41., Tan Jing, Yao Wenhua, Xu Chunxia, Luo Liming, kind emerging bright. the hereditary effect of 2008. high-quality protein maize grain characters. Acta Agronomica Sinica, 34 (5): 904-908., Fan, X.M., H.M. Chen, J. Tan, C.X. Xu, Y.M. Zhang, Y.X. Huang, and M.S. Kang. 2008. A New Maize Heterotic Pattern between Temperate and Tropical Germplasms. Agronomy Journal, 100 (3): 917-923., Yao Wenhua, Luo Liming, Wang Yanfen, yellow sky, section Chile, Xu Chunxia, Chen Hongmei, Tan Jing, kind emerging bright. 2009. utilize the SSR mark that 27 parts of corn inbred lines are carried out the hybrid vigour group divides. the corn science, 17(1): 54-58., X. M. Fan, Y. M. Zhang, W. H. Yao, H. M. Chen, J. Tan, C. X. Xu, X. L. Han, L. M. Luo, and M. S. Kang. 2009. Classifying Maize Inbred Lines into Heterotic Groups using a Factorial Mating Design. Agronomy Journal, 101:106 – 112., Tan Jing, Tian Junming, Zhang Xuemei, Bai Yongliu, Zhang Peigao, kind emerging bright. the hereditary effect analysis of 2010. high-quality protein maize Other Main Agronomic Characters. southwestern agriculture journal, 23 (4): 1032-1038., H.M. Chen, Y.D. Zhang, W. Chen, M.S. Kang, J. Tan, Y.F. Wang, J.Y. Yang, X.M. Fan. 2010. Improving Grain Yield And Yield Components Via Backcross Procedure. Maydica, 55:145-153. and Zhang Xuemei, Yao Wenhua, Tan Jing, Chen Hongmei, Xu Chunxia, Huang Bihua, kind emerging bright. 2010. temperate zone high oil corn self interweaving series and tropical corn inbred line Analysis of Combining Ability. corn science, 18 (2): open among the 5-10..
In the 42nd page of " the new variety of agricultural plant power protection communique " that CML171-1-1-2-1-1 has published on May 1st, 2007 the 3rd phase in 2007 record its be applied in the breeding as parent material.
The inventive method utilizes the high oil 115 of temperate zone high oil corn crossbreed for donor parents, good tropical corn inbred line CML171 for the feature of the tropical high oil corn self interweaving series CML171-1-1-2-1-1 of recurrent parent seed selection to be: seedling leaves green, leaf sheath green; The plant type hemicompact is gathered, 170 centimetres of plant heights, 108 centimetres of fringe positions, complete stool number of blade 18-19 sheet; Tassel branch 12-15, Ying's shell green, flower pesticide yellow, filigree white; Fruit ear is long tapered, 14.5 centimetres of average spike lengths, and thick 4.2 centimetres of fringe, tassel row number 10-12 is capable, the yellow solids of seed, cob white; Full seed crude fat 7.72%; About 128 days breeding times, anti-corn northern and southern leaf blight, head smut, ear rot and short mosaic disease, it is good to hold green property.
The feature of the high oil 115 of temperate zone high oil corn crossbreed is: sow in spring 115-125 days breeding time, indivedual high and cold mountain areas can extend to about 135 days; Plant type is open and flat, dark green leaf color, complete stool number of blade 21-23 sheet; Plant height 240-270 centimetre, the high 120-140 in fringe position centimetre, sense Maize Ear Rot and short mosaic disease; The long cartridge type of fruit ear, red axle, 19 centimetres of average spike lengths, thick 4.7 centimetres of fringe, tassel row number 14-16 are capable, and seed buff half horse flute profile, embryo are big, grain oil content 8.8%, protein content 11.3%, lysine content 0.33%, 100-grain weight 310 grams.
The feature of torrid zone corn inbred line CML171 is: seedling leaves green, leaf sheath green; The plant type hemicompact is gathered, 172 centimetres of plant heights, 112 centimetres of fringe positions, complete stool number of blade 18-19 sheet; Tassel branch 12-15, Ying's shell green, flower pesticide yellow, filigree white; Fruit ear is long tapered, 13.5 centimetres of average spike lengths, and thick 4.2 centimetres of fringe, tassel row number 10-12 is capable, the yellow solids of seed, cob white; Full seed crude fat 4.21%; About 130 days breeding times, anti-corn northern and southern leaf blight, head smut, ear rot and short mosaic disease, it is good to hold green property.
Compared with the prior art, the invention has the beneficial effects as follows:
The present invention is by hybridizing with temperate zone high oil corn crossbreed and tropical corn inbred line, backcross and the tropical high oil corn self interweaving series of selfed breeding, it is narrow to overcome prior art seed selection high oil corn blastogenesis basis, lack and adapt to the torrid zone, the high oil corn germplasm of subtropical zone, the general disease resistance of the high-oil corn variety of breeding is relatively poor, output is lower, be difficult to suit in the torrid zone, the defective that apply the subtropical zone, the floorboard with high oil content of temperate zone high oil corn germplasm and the excellent resistance of tropical corn germplasm have been concentrated with the tropical high oil corn self interweaving series that the inventive method is bred, the performance product are of fine quality, better resistance and output height, particularly have a clear superiority at more original temperate zone high oil corn aspect resistance and the output, widened the germplasm basis of temperate zone high oil corn, for seed selection adapts to the torrid zone, the high oil corn crossbreed of subtropical zone is had laid a good foundation.Reach 7.72%(in mass fraction as the tropical high oil corn self interweaving series CML171-1-1-2-1-1 grain oil content of breeding with the inventive method, be that full seed crude fat content is 7.72%), show disease-resistant again and the output height, the high-resistance corn northern and southern leaf blight is (big, the sick level of helminthosporium maydis is 2.5 grades), high-resistance corn head smut (the head smut incidence of disease is 0), high-resistance corn fringe Maize Ear Rot (fringe Maize Ear Rot average sick level be 1) and in anti-corn short mosaic disease (the short mosaic disease incidence of disease is 20.6%), in the torrid zone, Yunnan, the subtropical zone average yield can reach 320 kilograms/mu.
Embodiment
Further specify the present invention below in conjunction with embodiment, but be not construed as limiting the invention.
Embodiment 1
Introduced the high oily 115(of temperate zone high oil corn crossbreed in mass fraction from China Agricultural University in (1) 1996 year, full seed crude fat 8.8%) in Kunming, Yunnan (25 ° 12 of north latitude ', the subtropical zone) plants experimentally, high sense Maize Ear Rot and short mosaic disease, average yield per mu is promoted single No. 4 underproduction 9.4% of ordinary corn hybrid species meeting than the Yunnan large tracts of land;
(2) introduce tropical corn inbred line CML171(in mass fraction from the international corn wheat improvement center of Mexico (CIMMYT) the same year, full seed crude fat 4.21%) in Kaiyuan City, Yunnan (23 ° 71 of north latitude ', the subtropical zone) plants experimentally, the performance plant is delicate and pretty, disease-resistant, the seed commodity is good and output is high, the yellow solids of seed, anti-corn northern and southern leaf blight, head smut, fringe Maize Ear Rot and short mosaic disease etc., output reaches 316 kilograms/mu;
(3) 1996 year winter, (north latitude 21 ° 99 ', torrid areas) be that female parent, tropical corn inbred line CML171 are male parent with the high oil 115 of temperate zone high oil corn crossbreed in the Jinghong, Yunnan, the oily 115/ CML171 F of hybridization assembly acquisition height
1Seed;
(4) 1997 year spring be the high oily 115/ CML171 F of (north latitude 23 ° 71 ', subtropical zone) plantation simultaneously in Kaiyuan City, Yunnan
1And CML171, get CML171 pollen flowering stage and award high oily 115/ CML171 F
1, obtain high oily 115/ CML171//CML171 BC
1Seed;
(5) to the oily 115/ CML171//CML171 BC of height
1Seed carries out evaluation and screening, select the embryo plane account for the seed of seed plane more than 3/4 in winter planting in 1997 in the Jinghong, Yunnan (north latitude 21 ° 99 ', torrid areas), plant CML171 simultaneously; Get CML171 pollen flowering stage and award high oily 115/ CML171//CML171 BC
1, obtain high oily 115/ CML171//CML171/ // CML171 BC
2Seed;
(6) to oily 115/ CML171//CML171/ of height //CML171 BC
2Seed carries out evaluation and screening, select the embryo plane to account for the seed of seed plane 3/4 or more and plant in Kunming, Yunnan (north latitude 25 ° 12 ', subtropical zone) spring in 1998, the selfing in flowering stage obtain oily 115/ CML171//CML171/ of height //CML171 BC
2S
1Seed;
(7) to oily 115/ CML171//CML171/ of height //CML171 BC
2S
1Seed carries out evaluation and screening, select the embryo plane account for the seed of seed plane 3/4 or more in winter planting in 1998 in the Jinghong, Yunnan (north latitude 21 ° 99 ', torrid areas), the selfing in flowering stage obtain oily 115/ CML171//CML171/ of height //CML171 BC
2S
2Seed;
(8) to oily 115/ CML171//CML171/ of height //CML171 BC
2S
2Seed carries out evaluation and screening, select the embryo plane to account for the seed of seed plane 3/4 or more and plant in Kunming, Yunnan (north latitude 25 ° 12 ', subtropical zone) spring in 1999, the selfing in flowering stage obtain oily 115/ CML171//CML171/ of height //CML171 BC
2S
3Seed;
(9) to oily 115/ CML171//CML171/ of height //CML171 BC
2S
3Seed carries out evaluation and screening, select the embryo plane account for the seed of seed plane 3/4 or more in winter planting in 1999 in the Jinghong, Yunnan (north latitude 21 ° 99 ', torrid areas), the selfing in flowering stage obtain oily 115/ CML171//CML171/ of height //CML171 BC
2S
4Seed;
(10) to oily 115/ CML171//CML171/ of height //CML171 BC
2S
4Seed carries out evaluation and screening, select the embryo plane to account for the seed of seed plane 3/4 or more and plant in Kunming, Yunnan (north latitude 25 ° 12 ', subtropical zone) spring in 2000, the selfing in flowering stage obtain oily 115/ CML171//CML171/ of height //CML171 BC
2S
5Seed;
(11) through national corn improvement central laboratory to oily 115/ CML171//CML171/ of height //CML171 BC
2S
5Seed carries out oil content to be measured, and the full seed crude fat of wherein numbering the CML171-1-1-2-1-1 family reaches 7.72%, reaches 2 grades of quality standards of national high oil corn (NY/T521-2002, crude fat (butt) 〉=7.5%).CML171-1-1-2-1-1 has concentrated the advantage of temperate zone high oil corn and tropical corn, both kept higher grain oil content (all in mass fraction, its grain oil content is 7.72%, it is full seed crude fat content 7.72%,), show disease-resistant again and the output height, the high-resistance corn northern and southern leaf blight is (big, the sick level of helminthosporium maydis is 2.5 grades), high-resistance corn head smut (the head smut incidence of disease is 0), high-resistance corn fringe Maize Ear Rot (fringe Maize Ear Rot average sick level be 1) and in anti-corn short mosaic disease (the short mosaic disease incidence of disease is 20.6%), in Kunming, Yunnan (north latitude 25 ° 12 '), Muller (north latitude 24 ° 41 '), Kaiyuan City's (north latitude 23 ° 71 '), build water (north latitude 23 ° 65 '), the torrid zones such as Jinghong (north latitude 21 ° 99 '), the average yield of subtropical zone can reach 320 kilograms/mu.
(12) utilize tropical high oil corn self interweaving series CML171-1-1-2-1-1, seed selection auspicious No. 8 of tropical high oil corn crossbreed cloud.According to the test of grain quality supervision and inspection center of the Ministry of Agriculture, No. 8 full seed crude fat 9.16% of Yun Rui reach 1 grade of quality standard of national high oil corn (NY/T521-2002, crude fat (butt) 〉=8.5%); In calendar year 2001 Yunnan Province academy of agricultural sciences's varietal yield test, 656.5 kilograms of No. 8 per mu yields of Yun Rui, than fine three volume increase 10.4% of first contrast, than excellent 167 volume increase 13.9% of the second contrast cloud, shelter has first of the combination of participating in the experiment; In 2002-2003 Yunnan Province corn variety regional test (C group), No. 8 two annual per mu yields of Yun Rui 562.3kg, output and conventional corn contrast kinds emerging yellow single 892 is suitable, and the point of contrast volume increase has 23, accounts for 54.8% of test site time; Identify that according to the disease-resistant worm of Institute of Crop Science, Chinese Academy of Agricultural Science's crop resource seminar identifies, the sick level of the auspicious No. 8 large and small pintas of cloud is 3 grades (high anti-), the short mosaic disease incidence of disease is anti-among the 29.2%(), the average sick level of ear rot is that 1(is high anti-), the head smut incidence of disease is that 0(is high anti-).Cloud auspicious No. 8 at present in Yunnan, Guizhou, Sichuan large scale application, liked by mass peasants, become the kind of promoting mainly in Yunnan Province's Maize Production.
Claims (2)
1. the selection of a tropical high oil corn self interweaving series, it is characterized in that: with temperate zone high oil corn crossbreed is that donor parents, tropical corn inbred line are recurrent parent, by 1 generation hybridization, in 2 generations, backcrossed and the selfing of 5 generations, hybridizing and backcrossing is male parent with tropical corn inbred line all, backcross simultaneously and selfing per generation is selected big embryo seed, seed selection torrid zone high oil corn self interweaving series, described big embryo seed is that the embryo plane accounts for the seed of seed plane more than 3/4.
2. the selection of a kind of tropical high oil corn self interweaving series according to claim 1 is characterized in that: described temperate zone high oil corn crossbreed is high oil 115, and described tropical corn inbred line is CML171.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919118A (en) * | 2012-11-21 | 2013-02-13 | 广西大学 | Breeding method of oil-purposed micro-endosperm maize inbred line with oil content being higher than 30% |
| CN103026963A (en) * | 2012-12-28 | 2013-04-10 | 四川雅玉科技开发有限公司 | Breeding method for temperate corn employing tropical germplasm |
| CN108235943A (en) * | 2016-12-26 | 2018-07-03 | 云南省农业科学院粮食作物研究所 | A kind of breeding method of high oiling torrid zone corn |
| CN116724883A (en) * | 2023-07-13 | 2023-09-12 | 云南省农业科学院粮食作物研究所 | Breeding method of high-oil waxy corn |
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2011
- 2011-06-11 CN CN2011101559813A patent/CN102217531A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919118A (en) * | 2012-11-21 | 2013-02-13 | 广西大学 | Breeding method of oil-purposed micro-endosperm maize inbred line with oil content being higher than 30% |
| CN103026963A (en) * | 2012-12-28 | 2013-04-10 | 四川雅玉科技开发有限公司 | Breeding method for temperate corn employing tropical germplasm |
| CN108235943A (en) * | 2016-12-26 | 2018-07-03 | 云南省农业科学院粮食作物研究所 | A kind of breeding method of high oiling torrid zone corn |
| CN116724883A (en) * | 2023-07-13 | 2023-09-12 | 云南省农业科学院粮食作物研究所 | Breeding method of high-oil waxy corn |
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Application publication date: 20111019 |