CN102919118A - Breeding method of oil-purposed micro-endosperm maize inbred line with oil content being higher than 30% - Google Patents
Breeding method of oil-purposed micro-endosperm maize inbred line with oil content being higher than 30% Download PDFInfo
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- CN102919118A CN102919118A CN2012104749123A CN201210474912A CN102919118A CN 102919118 A CN102919118 A CN 102919118A CN 2012104749123 A CN2012104749123 A CN 2012104749123A CN 201210474912 A CN201210474912 A CN 201210474912A CN 102919118 A CN102919118 A CN 102919118A
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Abstract
The invention provides a breeding method of an oil-purposed micro-endosperm maize inbred line with oil content being higher than 30%. According to the breeding method, a nuclear magnetic resonance spectrometer is used for selecting micro-endosperm maize seeds with oil content being higher than 24% to form an original population; and after 8-10 rounds of mass selection and improvement, a superhigh-oil micro-endosperm maize population with oil content being higher than 30%; the inbred line is separated from the superhigh-oil micro-endosperm maize population; and through multiple generations of self breeding, the micro-endosperm maize inbred line with oil content being stably higher than 30% is obtained.
Description
Technical field
The present invention relates to a kind of oil content and use the selection of type micro-endosperm corn inbred line greater than 30% oil, belong to plant variety seed selection field.
Background technology
Corn oil is high-quality edible oil, and its main component is unsaturated fatty acid, and wherein oleic acid and linoleic acid account for more than 80%, and is rich in vitamin E, phytosterol, phosphatide etc.Corn oil is called as " health-nutrition oil ", has the function that reduces cholesterol and low-density lipoprotein content, can play reducing blood lipid, softening blood vessel, prevention cardiovascular disease, improves immunity of organisms, delay senility.
The production of corn oil is along with the breeding and promoting of high-oil corn variety, and the edible oil that grows up gradually.By the industry standard NY/T521-2002 of Ministry of Agriculture regulation, oil content is higher than 6% corn, can be referred to as high oil corn.But because high oil corn is still to produce starch as main, corn oil is as the by product of producing starch or production corn ethanol, and to produce corn oil with common high oil corn, must first embryo be separated again lixiviate or squeeze corn oil from embryo from seed.Manufacturing procedure is complicated, and processing cost is high, and in process, the natural nutrient component loss is serious.
In order to simplify the manufacturing procedure of corn oil, can directly press corn oil, and can keep the natural organic nutrition composition of corn oil.Corn seminar of Guangxi University has selected a kind of micro-endosperm corn germplasm through the breeding of more than ten years, and the ripe seed of this corn is comprised of pericarp, aleurone layer and embryo, does not substantially contain the storage endosperm.The mature seed oil content of this corn can be used as novel oil crop and directly squeezes corn oil with whole kernel corn greater than 20%.Because corn is to utilize the earliest heterotic crop, therefore all have more the improvement prospect than any other oil crop.But the highest oil content of the micro-endosperm corn hybrid combination of institute's assembly is 27% at present, not yet breaks through 30%.In order to improve micro-endosperm corn as the economic benefit of oil crop, need further to improve the oil content that crossbreed is used in micro-endosperm corn production, at first the micro-endosperm corn inbred line of the higher oil content of seed selection (greater than 30%) is hybridized assembly as the parent for this reason.But by the method for the general Breeding of Inbred Lines such as pedigree method, the oil content of selected micro-endosperm corn inbred line is up to 26%, can not break through 30%.
Summary of the invention
The purpose of this invention is to provide a kind of oil content greater than 30% the oil selection with type micro-endosperm corn inbred line, by the present invention, can select oil content greater than 30% micro-endosperm corn inbred line.
The inventor's corn seminar finds after deliberation, there is widely variation in the oil content of micro-endosperm corn, and show by multiple genetic design methods analysts such as Major genes plus polygenes inheritance model analysis, hereditary effect analysis, Analysis of Combining Ability, the oil content of micro-endosperm corn has very high heritability, and this explanation is feasible by the oil content that the method for mixing the recurrent selections such as selection further improves micro-endosperm corn.The inventor is based on this principle, cultivated oil content greater than 30% micro-endosperm corn inbred line.
Technical scheme of the present invention is:
Oil content is used the selection of type micro-endosperm corn inbred line greater than 30% oil, comprise the steps:
A. initial population is synthetic: utilize the various corn germ plasm resources of collecting, comprise conventional corn, sweet corn, waxy corn and common high oil corn etc., utilize their intergenic mutual works, carry out multiple hybridization between all kinds corn, therefrom select the corn material of little endosperm type, from these micro-endosperm corn materials, select the preferably plant institute seed of fringe as a result of economical character, then utilize NMR to select oil content greater than 24% micro-endosperm corn seed, fully select the seed of more fruit ear, synthetic micro-endosperm corn initial population as far as possible;
B. the mixing of many samsaras is selected: the oil content that step a is selected carries out isolation implant greater than 24% micro-endosperm corn seed, open-pollinated, approximately 1500 strains of plantation micro-endosperm corn, carry out to mix and select, after the maturation, select seed fuller and without the fruit ear of damage by disease and insect, natural air drying, single fringe threshing, each fruit ear is got 3 samples, each sample is got 5 corn seeds at random, after measuring 5 weights with electronic balance, use again the nmr determination oil content, select oil content greater than 25% and the heavy heavier fruit ear of grain, isolation implant, open-pollinated allows their gene do fully mutually, carries out the mixing of a new round after the maturation and selects.Along with samsara algebraically increases, progressively improve the oil content of fruit ear, progressively select oil content greater than 28% and the heavy heavier fruit ear of grain, select plantation through the mixing of 8-10 wheel, the average oil content of more fruit ear is arranged greater than 30% in the colony that obtains;
C. separate inbred line: mix the micro-endosperm corn colony that obtains after the selection through the 8-10 samsara, therefrom select the good individual plant of economical character, selfing, every continuously selfing 4-6 is for once handing over each other, in per generation, selected oil content greater than 30%, and the seed of the fruit ear that economical character is good is as the material of next generation's plantation.Until the major traits of each fruit ear of obtaining of plantation is when basically identical, conservation or suitably take to hand over mutually each other in being after, conservation.Can obtain thus oil content stable greater than little endosperm Super-high Oil Maize inbred line of 30%.
The benefit that the present invention produces is:
The micro-endosperm corn inbred line oil content that selects by method of the present invention has raising by a relatively large margin.The oil content majority that passes through at present the micro-endosperm corn inbred line of this kind method seed selection surpasses 30%, and the oil content of the inbred line that an existing comprehensive agronomy the Characters is good reaches 32.7%; Other has a strain, and the highest oil content of single fringe is 37.25%, and the highest oil content of simple grain is 39.68%.Therefore use method of the present invention with these inbred lines as the parent, be used for assembly, cultivate the micro-endosperm corn crossbreed of higher oil content, can further improve micro-endosperm corn as the oil production of oil crop, increase economic efficiency.
Embodiment
Below in conjunction with embodiment effect of the present invention is described in detail:
Utilize the various corn germ plasm resources of collecting, comprise conventional corn, sweet corn, waxy corn and common high oil corn etc., utilize intergenic mutual work, carry out multiple hybridization between all kinds, therefrom select little endosperm type, and its bad economical character constantly improved, obtain the micro-endosperm corn material.Utilize NMR from these micro-endosperm corn materials, to select oil content greater than 24% micro-endosperm corn seed, they are mixed sowing, isolation open-pollinated, select seed fuller and without the fruit ear of damage by disease and insect after ripe, behind the results natural air drying, use the nmr determination oil content, select oil content greater than 25% fruit ear, threshing, mix, continue the next round plantation and carry out mixing, the selection of a new round, so carry out the mixing of such 8 samsaras and select.Along with the increase of samsara algebraically, progressively select oil content greater than 28% and the heavy heavier fruit ear of grain, therefrom select again oil content greater than 30% simple grain, mix the high oily colony of a synthetic new round.From mix the little endosperm Super-high Oil Maize colony of selecting improvement through too much samsara, select oil content greater than 30% corn kernel, sowing is when plant strain growth is weaved silk the phase to loose powder, the bagging selfing, select agronomy after the maturation better, oil content is greater than 30% seed, as the seed of selfing of future generation, whenever carry out 4-6 for selfing, carry out handing over each other in the subsystem, until each major traits of fruit ear is basically identical, the acquisition oil content is stablized the little endosperm Super-high Oil Maize inbred line seed greater than 30%.Take 5 oil contents of measuring the selfing fruit ear as unit, wherein the oil content of 10 micro-endosperm corn inbred lines is shown in the second row of table 1 by NMR;
Adopt conventional pedigree method, after namely selecting to produce micro-endosperm corn by the resulting second filial generation of all kinds corn intermolecular hybrid, select good individual plant, the plantation single-strain seed becomes strain, by going out the micro-endosperm corn inbred line for selfed breeding, take 5 oil contents of measuring fruit ear as unit, wherein the oil content of 10 micro-endosperm corn inbred lines is shown in the first row of table 1 by NMR;
The comparison of the oil content of the micro-endosperm corn inbred line that table 1 the inventive method and conventional pedigree method select
Data from table find out, adopt the oil content of the micro-endosperm corn inbred line that the inventive method selects all to surpass 30%, and oil content is largely increased than pedigree method.
Claims (1)
1. an oil content is characterized in that: may further comprise the steps greater than 30% the oil selection with type micro-endosperm corn inbred line:
A. initial population is synthetic: conventional corn, sweet corn, waxy corn and the mixed planting of common high oil corn seed, carry out multiple hybridization between all kinds corn, therefrom select the corn material of little endosperm type after the maturation; From these micro-endosperm corn materials, select the preferably plant institute seed of fringe as a result of economical character, then utilize NMR to select oil content greater than 24% micro-endosperm corn seed, be micro-endosperm corn initial population;
B. the mixing of many samsaras is selected: the oil content that step a is selected carries out isolation implant, open-pollinated greater than 24% micro-endosperm corn seed; After the maturation, select seed fuller and without the fruit ear of damage by disease and insect, natural air drying, single fringe threshing; Use the nmr determination oil content, select oil content greater than 25% and the heavy heavier fruit ear of grain, isolation implant, open-pollinated carries out the mixing of a new round and selects after the maturation; Along with samsara algebraically increases, progressively improve the oil content of fruit ear, progressively select oil content greater than 28% and the heavy heavier fruit ear of grain, select plantation through the mixing of 8-10 wheel, the average oil content of more fruit ear is arranged greater than 30% in the colony that obtains;
C. separate inbred line: mix the little endosperm Super-high Oil Maize colony that obtains after selecting through the 8-10 samsara, therefrom select the good individual plant of economical character, selfing, every continuously selfing 4-6 is for once handing over each other, in per generation, selected oil content greater than 30%, and the seed of the fruit ear that economical character is good is as the material of next generation's plantation; Until the major traits of each fruit ear of obtaining of plantation is when basically identical, conservation or suitably take to hand over mutually each other in being after conservation, can obtain thus oil content stable greater than little endosperm Super-high Oil Maize inbred line of 30%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197574A (en) * | 2018-11-13 | 2019-01-15 | 南京林业大学 | A kind of oil selection of tree peony fine individual plant |
| CN110305733A (en) * | 2019-06-26 | 2019-10-08 | 广西壮族自治区林业科学研究院 | A method of corn oil is prepared using corn whole grain |
| CN112056209A (en) * | 2020-08-11 | 2020-12-11 | 沈阳特亦佳玉米科技有限公司 | Popcorn breeding method for improving puffing coefficient and improving flower shape |
| CN113508749A (en) * | 2021-08-24 | 2021-10-19 | 云南珍禾丰种业有限公司 | High-oil waxy corn hybridization breeding method |
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| CN1401222A (en) * | 2002-09-16 | 2003-03-12 | 吴子恺 | Method for breeding microendosperm supersweet superhigh oil content dualpurpose maize |
| CN1443441A (en) * | 2003-01-06 | 2003-09-24 | 黄炳生 | High-oil high-quality protein glutinous corn intercross guality improvement and yield increment |
| CN102217531A (en) * | 2011-06-11 | 2011-10-19 | 云南省农业科学院粮食作物研究所 | Breeding method for tropical high-oil maize self-bred line |
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Patent Citations (3)
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| CN1401222A (en) * | 2002-09-16 | 2003-03-12 | 吴子恺 | Method for breeding microendosperm supersweet superhigh oil content dualpurpose maize |
| CN1443441A (en) * | 2003-01-06 | 2003-09-24 | 黄炳生 | High-oil high-quality protein glutinous corn intercross guality improvement and yield increment |
| CN102217531A (en) * | 2011-06-11 | 2011-10-19 | 云南省农业科学院粮食作物研究所 | Breeding method for tropical high-oil maize self-bred line |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197574A (en) * | 2018-11-13 | 2019-01-15 | 南京林业大学 | A kind of oil selection of tree peony fine individual plant |
| CN110305733A (en) * | 2019-06-26 | 2019-10-08 | 广西壮族自治区林业科学研究院 | A method of corn oil is prepared using corn whole grain |
| CN112056209A (en) * | 2020-08-11 | 2020-12-11 | 沈阳特亦佳玉米科技有限公司 | Popcorn breeding method for improving puffing coefficient and improving flower shape |
| CN112056209B (en) * | 2020-08-11 | 2022-12-27 | 沈阳特亦佳玉米科技有限公司 | Breeding method of cracked corn with improved expansion coefficient and improved flower shape |
| CN113508749A (en) * | 2021-08-24 | 2021-10-19 | 云南珍禾丰种业有限公司 | High-oil waxy corn hybridization breeding method |
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Application publication date: 20130213 |