CN105248275A - Breeding method of purple chili selfing line - Google Patents
Breeding method of purple chili selfing line Download PDFInfo
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- CN105248275A CN105248275A CN201510797110.XA CN201510797110A CN105248275A CN 105248275 A CN105248275 A CN 105248275A CN 201510797110 A CN201510797110 A CN 201510797110A CN 105248275 A CN105248275 A CN 105248275A
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Abstract
The invention discloses a breeding method of a purple chili selfing line and belongs to the technical field of plant breeding. The breeding method comprises the following steps: (1) preparing red F1-generation hybrid seeds by red and yellow chilies; (2) taking the generation F1 as a male parent or a female parent to be hybridized with green chilies to obtain three-way hybridized seeds; (3) taking the green chilies as a male parent or a female parent to carry out backcross on the red three-way hybridized seeds to prepare a generation BC1; (4) selecting green single plants from a BC1-generation segregation population and carrying out selfing to obtain BC1F2-generation seeds; (5) selecting purple chilies from a BC1F2-generation segregation population, carrying out selfing and reserving seeds; and (6) carrying out 3-5 generation selfing and homozygosis to obtain the purple chili selfing line. The invention provides a novel breeding manner for the establishment of purple chili germplasm resources, and purple chili hybrid seeds with obvious advantages can be further cultured by using the novel breeding manner; and the breeding method can be popularized and applied in the aspects of breeding and production.
Description
Technical field
The present invention relates to field of plant variety breeding technology, particularly relate to the selection of purple chili inbred line.
Background technology
Capsicum (CapsicumannuumL), Solanaceae, Capsicum are 1 year or limited herbaceos perennial.Fruit usually conically or Long Circle, in green during prematurity, becomes cerise, green or purple after maturation, the most common with redness.Capsicum is one of important in the world vegetable crop, whole world pepper planting area fifty-five million mu, and Chinese Capsicum cultivated area is 2,300 ten thousand mu is the first capsicum producing country and major consumers state in the world, also be that capsicum exports one of maximum country, average yield per mu is more than 1000 kilograms.
Existing purple chili kind is mainly the bird-caging pimento series that external seeds company produces, and China is mainly to consume capsicum; Simultaneously under capsicum generally carries out the condition of facility cultivation, existing kind can not meet the requirement of producing its tolerance to low temperature and weak light, disease resistance, commodity and high-quality, high yield; Existing goods green pepper pericarp look can not meet the requirement of consumer to capsicum pattern.Therefore, to be suitable for the novel purple hot pepper variety of facility cultivation significant in seed selection.
China Patent Publication No. CN103026962A, publication date on 04 10th, 2013, this scheme relates to a kind of breeding method of novel purple hot pepper variety, it is characterized in that: with purple bird-caging Sweet Pepper Varieties " Zuro " for maternal, green light cage type Sweet Pepper Varieties " Soviet Union green pepper No. 13 " is the combination of male parent preparing hybrid, selfing is carried out to hybrid combination " Zuro × Soviet Union green pepper No. 13 ", builds F2 colony; Plantation F
2plant, selects commodity pericarps purple, long bird-caging, setting ability is strong continuously individual plant, and carries out selfing and reserve seed for planting, and obtains F
3strain, to F
10stable for each strain proterties, obtain purple bird-caging pimento " 008 "; Purple bird-caging pimento " 008 " and " the yellow shell in Nanjing early green pepper " are by mixed planting, then be female parent with " Nanjing yellow shell early green pepper ", purple bird-caging pimento " 008 " carries out flower bud phase artificial emasculation pollination for male parent, and the ripening fruits of gather " the yellow shell in Nanjing early green pepper ", namely obtains " color green pepper No. 1 of reviving "
China Patent Publication No. CN102524045A, publication date on 07 04th, 2012, this scheme relates to a kind of selective breeding method of spicy purple chili, it is characterized in that: maternal inbred line 9708A: strong from the west of a river green pepper growth selection impetus, early stage fruit-setting rate high, disease-resistant 92011 for maternal, from Anqing early green pepper, select to begin that flower joint position is low, prematurity good, high 92105 the carrying out synthesis for male parent and hybridize of fruit-setting rate under low temperature and poor light condition, with precocity, tolerance to low temperature and weak light, the time of infertility fruit-setting rate high for target, carry out in offspring continuous 8 generation Single-plant selection cultivate into high for inbred line; Male parent inbred line 9718: growth selection gesture strong, purple phase long basis, the large Lantern of fruit, individual plant that fruit-setting rate is high from pimento purple high official offspring, reserves seed for planting with single fruit, carries out continuous 8 generations selfing and cultivates into and meet the stable inbred line of target call major traits; Fruit cattle horn shape, fruit shape is comparatively straight, and slightly cave in fruit face, good luster, and pericarp is thinner, and meat is thick medium; The medium pungent of fruit, excellent taste.
The cultivation period of documents one is oversize, and does not relate to the homozygous inbred lines of different genotype in documents two, we be intended to short by a kind of cultivation period, economical character good, the selection of the homozygous inbred lines of different genotype.
Summary of the invention
The present invention seeks to, the problem of and edible safety single for the genotype existing for existing purple chili, provide that a kind of cultivation period is short, economical character good, the method for the homozygous inbred lines purple chili germ plasm resource of different genotype.
The object of the invention is achieved by the following technical programs.
A selection for purple chili inbred line, the method is carried out according to the following steps:
1) make male parent or female parent and yellow capsicum by red chili to hybridize, obtaining fruit color is red F
1for crossbreed;
2) by F
1for crossbreed as male parent or maternal hybridize with green capsicum, obtaining fruit color is triple hybrid that is red and yellow;
3) using green capsicum as male parent or female parent, be that red triple hybrid backcrosses and obtains the BC that backcrosses to fruit color
1generation;
4) BC
1in generation, is separated into three types of populations that fruit color is respectively red, yellow and green, therefrom selects the excellent green capsicum individual plant of Agronomic to carry out selfing and reserves seed for planting, obtain BC
1f
2for seed;
5) BC
1f
2in generation, is separated into fruit color and is respectively colony that is green and purple, therefrom selects the excellent purple chili individual plant of economical character to carry out selfing and reserves seed for planting;
6) above-mentioned selfed seed is carried out again after 3-5 isozygotys for selfing, therefrom selecting the purple chili inbred line obtaining color, economical character stable and consistent.
Described red chili is from T09-028, red rich 404 and the capsicum variety of choosing any one kind of them towards sky 148.
Described yellow capsicum choosing is the capsicum variety of choosing any one kind of them from T09-023 and CH05-019.
Described green capsicum is CH08-003.
The invention has the beneficial effects as follows:
1, the present invention utilizes existing germ plasm resource by hybridizing, backcrosses, selfing, the technological means such as separation selection carry out genetic recombination, create a kind of new method utilizing the capsicum material hybridization of redness, yellow and green three kinds of different colours to cultivate purple chili germ plasm resource, the establishment for purple chili germ plasm resource provides a kind of new breeding methods.
2, the purple chili inbred line germ plasm resource that the present invention cultivates has the genotype different from existing purple kind, utilizes it can cultivate into new purple chili crossbreed as parent material, more can fully demonstrate hybrid vigour;
3, the present invention can be the further investigation of pepper fruit color genetic development, provides new material base.
Embodiment
By following examples, the present invention is described in further detail, the explanation to material involved by following examples:
Red chili material: T09-028 is pungent red No. 1, introduces from Agricultural in Chongqing Science Institute; ' red rich 404 ' product germlines are introduced by Hong Kong Yi Nong company; Introduced towards sky 148 by Agricultural in Chongqing Science Institute;
Yellow capsicum material: T09-023 original name " color No. 1 of peace ", introduces from Anyang vegetables Science Institute; CH05-019 original name " new green pepper No. 19 ", introduces from Bayangolmongol Autonomous Prefecture Institute of agricultural sciences;
Green capsicum material: CH08-003 original name " rich green pepper No. 1 ", introduces from Lanzhou City, Gansu Province Run Fengzhong industry Co., Ltd.
The seed selection of embodiment 1:(purple chili CL1-1, CL1-2 inbred line)
1) the 5-8 month in 2009: with red chili T09-028 for female parent, hybridize for male parent with yellow capsicum No. T09-023, obtain F
1for material 2823, fruit color is red;
2) in March ,-2010 in September, 2009: with green capsicum CH08-003 for female parent, with 2823 for male parent is hybridized, obtain triple hybrid 08-003 × 2823, fruit color is red and yellow;
3) the 5-8 month in 2010: be that samsara is maternal with CH08-003, in 08-003 × 2823, red plant is male parent, carries out hybridization and obtains first backcross generation 08-003 × 2823BC
1;
4) in March ,-2011 in September, 2010: plantation first backcross generation plant 150 strain, fruit color is separated into redness, yellow and green three kinds, and therefrom growth selection, result show excellent green capsicum individual plant and carry out selfing and reserve seed for planting, and obtain BC
1f
2for seed;
5) in August ,-2011 in May, 2011: plantation BC
1f
2plant 300 strain, fruit color is separated into green and purple, and therefrom growth selection, result show excellent purple chili individual plant, and selfing is reserved seed for planting;
6) in August ,-2013 in September, 2011: obtain stable purple chili inbred line through continuous selfing 4 generations of isozygotying, therefrom select individual plant CL1-1, CL1-2 that proterties is excellent.
The seed selection of embodiment 2:(purple chili CL2-1, CL2-2 inbred line)
1) in March ,-2010 in September, 2009: with yellow capsicum CH05-019 for female parent, with red chili red rich 404 for male parent is hybridized, the F obtained
1be 1904 for material number, fruit color is red;
2) the 5-8 month in 2010: with green capsicum CH08-003 for female parent, with 1904 for male parent is hybridized, obtain triple hybrid 08-003 × 1904, fruit color is red and yellow;
3) in March ,-2011 in September, 2010: be maternal with plant red in 08-003 × 1904, take CH08-003 as samsara male parent, carry out hybridization and obtain first backcross generation 08-003 × 1904BC
1;
4) the 5-8 month in 2011: plantation first backcross generation 08-003 × 1904BC
1plant 150 strain, fruit color is separated into redness, yellow and green three kinds, and growth selection, result show excellent green capsicum individual plant selfing and reserve seed for planting, and obtain BC
1f
2for seed;
5) in March ,-2012 in September, 2011: plantation BC
1f
2plant 300 strain, fruit color is separated into green and purple, and therefrom growth selection, result show excellent purple chili individual plant, and selfing is reserved seed for planting;
6) in August ,-2014 in May, 2012: obtain stable purple chili inbred line through continuous selfing 5 generation, therefrom select individual plant CL2-1, CL2-2 that proterties is excellent.
The seed selection of embodiment 3:(purple chili CL3-1, CL3-2 inbred line)
1) the 5-8 month in 2010: be maternal towards sky 148 with red chili, with yellow capsicum CH05-019 for male parent is hybridized, F is obtained
1be 4819 for material number, fruit color is red;
2) in March ,-2011 in September, 2010: be maternal with 4819, with green capsicum CH08-003 for male parent is hybridized, obtain triple hybrid 4819 × 08-003, fruit color be redness and yellow;
3) the 5-8 month in 2011: be maternal with plant red in 4819 × 08-003 is that samsara male parent is carried out hybridization and obtained first backcross generation 4819 × 08-003BC with CH08-003
1;
4) in March ,-2012 in September, 2011: plantation first backcross generation 4819 × 08-003BC
1plant 150 strain, fruit color is separated into redness, yellow and green three kinds, and therefrom growth selection, result show excellent green capsicum individual plant selfing and reserve seed for planting, and obtain BC
1f
2for seed;
5) the 5-8 month in 2012: plantation BC
1f
2plant 300 strain, fruit color is separated into green and purple, and therefrom growth selection, result show excellent purple chili individual plant, carry out selfing and reserve seed for planting;
6) in August ,-2014 in September, 2012: obtain stable purple chili inbred line through continuous selfing 4 generation, therefrom select individual plant CL3-1, CL3-2 that proterties is excellent.
The seed selection of embodiment 4:(purple chili CL4-1, CL4-2 inbred line)
1) in March ,-2010 in September, 2009: with yellow capsicum T09-023 for female parent, with red chili towards sky 148 for male parent is hybridized, the F obtained
1be 2348 for material number, fruit color is red;
2) the 5-8 month in 2010: be maternal with 2348, with green capsicum CH08-003 for male parent is hybridized, obtain triple hybrid 2348 × 08-003, fruit color be redness and yellow;
3) in March ,-2011 in September, 2010: be female parent with CH08-003, in 2348 × 08-003, red plant is male parent, carries out hybridization and obtains first backcross generation 2348 × 08-003BC
1;
4) the 5-8 month in 2011: the strain of plantation first backcross generation plant 150, fruit color is separated into redness, yellow and green three kinds, and therefrom growth selection, result show excellent green capsicum individual plant selfing and reserve seed for planting, and obtain BC
1f
2for seed;
5) 9-2012 March in 2011: plantation BC
1f
2plant 300 strain, fruit color is separated into green and purple, and therefrom growth selection, result show excellent purple chili individual plant, carry out selfing and reserve seed for planting;
6) in August ,-2013 in May, 2012: obtain stable purple chili inbred line through continuous selfing 3 generation, therefrom select individual plant CL4-1, CL4-2 that proterties is excellent.
The purple cloud of the cultivation kind on market and the present invention is selected to contrast, as table one.
Table one
As shown in Table 1, the effect of embodiment 3 is the most obvious.Therefore, adopt red chili towards sky 148, the Breeding Effect of purple chili inbred line that yellow capsicum CH05-019 and green capsicum CH08-003 carries out is the most remarkable.Its per mu yield reaches 3450 kilograms, plant height 85cm, fruit-setting rate 87%, and average fruit weight reaches 1701.8mg/kg, and rate of growth is 32.7%.
Claims (4)
1. a selection for purple chili inbred line, is characterized in that carrying out according to the following steps:
1) with from T09-028, red rich 404 and the red chili of choosing any one kind of them in sky 148 make male parent or female parent, hybridize with the yellow capsicum of choosing any one kind of them from T09-023 and CH05-019, obtaining fruit color is red F
1for crossbreed;
2) by F
1for crossbreed as male parent or maternal hybridize with green capsicum CH08-003, obtaining fruit color is triple hybrid that is red and yellow;
3) using green capsicum CH08-003 as male parent or female parent, be that red triple hybrid backcrosses and obtains the BC that backcrosses to fruit color
1generation;
4) BC
1in generation, is separated into three types of populations that fruit color is respectively red, yellow and green, therefrom selects the excellent green capsicum individual plant of Agronomic to carry out selfing and reserves seed for planting, obtain BC
1f
2for seed;
5) BC
1f
2in generation, is separated into fruit color and is respectively colony that is green and purple, therefrom selects the excellent purple chili individual plant of economical character to carry out selfing and reserves seed for planting;
6) selfed seed is carried out again after 3-5 isozygotys for selfing, therefrom selecting the purple chili inbred line obtaining color, economical character stable and consistent;
Wherein, described red chili T09-028 original name " No. 1, Xin Hong ", described yellow capsicum T09-023 original name " color No. 1 of peace ", introduces from Anyang vegetables Science Institute; CH05-019 original name " new green pepper No. 19 ", described green capsicum CH08-003 original name " rich green pepper No. 1 ".
2. the selection of a kind of purple chili inbred line as claimed in claim 1, is characterized in that described red chili is from T09-028, red rich 404 and the capsicum variety of choosing any one kind of them towards sky 148.
3. the selection of a kind of purple chili inbred line as claimed in claim 1, is characterized in that described yellow capsicum choosing is the capsicum variety of choosing any one kind of them from T09-023 and CH05-019.
4. the selection of a kind of purple chili inbred line as claimed in claim 1, is characterized in that described green capsicum is CH08-003.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105613274A (en) * | 2016-02-01 | 2016-06-01 | 何其家 | Rapid selfing isolation method for deep yellow sweet peppers in greenhouses |
| CN106069716A (en) * | 2016-06-15 | 2016-11-09 | 湖南省蔬菜研究所 | A kind of method of seed selection excellent yellow pepper resource material |
| CN107047283A (en) * | 2017-03-22 | 2017-08-18 | 上海交通大学 | A kind of pyramiding breeding method using color colour as colored French beans seed selection standard |
| CN107173219A (en) * | 2017-05-12 | 2017-09-19 | 衢州市农业科学研究院 | A kind of method of the excellent white pepper breeding material of seed selection |
-
2015
- 2015-11-18 CN CN201510797110.XA patent/CN105248275A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105613274A (en) * | 2016-02-01 | 2016-06-01 | 何其家 | Rapid selfing isolation method for deep yellow sweet peppers in greenhouses |
| CN106069716A (en) * | 2016-06-15 | 2016-11-09 | 湖南省蔬菜研究所 | A kind of method of seed selection excellent yellow pepper resource material |
| CN106069716B (en) * | 2016-06-15 | 2019-04-02 | 湖南省蔬菜研究所 | A kind of method of the excellent yellow pepper resource material of breeding |
| CN107047283A (en) * | 2017-03-22 | 2017-08-18 | 上海交通大学 | A kind of pyramiding breeding method using color colour as colored French beans seed selection standard |
| CN107047283B (en) * | 2017-03-22 | 2019-03-12 | 上海交通大学 | A kind of take color color value as the pyramiding breeding method of colored hyacinth bean breeding standard |
| CN107173219A (en) * | 2017-05-12 | 2017-09-19 | 衢州市农业科学研究院 | A kind of method of the excellent white pepper breeding material of seed selection |
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Application publication date: 20160120 |