CN1817103A - Double-line hybrid breeding method for marigold with pigment - Google Patents
Double-line hybrid breeding method for marigold with pigment Download PDFInfo
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- CN1817103A CN1817103A CN 200610059056 CN200610059056A CN1817103A CN 1817103 A CN1817103 A CN 1817103A CN 200610059056 CN200610059056 CN 200610059056 CN 200610059056 A CN200610059056 A CN 200610059056A CN 1817103 A CN1817103 A CN 1817103A
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- male sterile
- marigold
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- pigment
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Abstract
A two-line hybridization method for culturing marigold used as the raw material of natural pigment (especially the xanthophyll) includes such steps as culturing the male sterile bimotype line from the natural male sterile marigold, and crossing with the selfing line as male parent to obtain the crossed variety F1, which features high yield of seeds and strong heterotic vigor.
Description
Technical field the present invention relates to a kind of method of utilizing male sterile preparing hybrid kind, is a kind of double-line hybrid breeding method for marigold with pigment specifically.
The background technology marigold with pigment is a composite family Tagetes annual herb plant, its fresh flower is the raw material that extracts natural carotenol, xanthophyll is a kind of powerful antioxidant, be mainly used in medicine, food, industries such as feed chemical industry, about 2,000,000 mu of the cultivated area in whole world every year, about 600,000 mu of China, at present, the seed of marigold with pigment all is to adopt conventional variety both at home and abroad, and mainly from U.S.'s import, the output of its fresh flower is on the low side, general per mu yield fresh flower 1500-2000 kilogram, pigment content 15-20g/kg, disease resistance is relatively poor, is badly in need of high yield in the production, disease-resistant, the crossbreed that pigment content is high replaces conventional variety.
Summary of the invention the object of the present invention is to provide a kind of double-line hybrid breeding method for marigold with pigment, can utilize heterosis, hybrid vigor with this method, cultivates the hybrid seed of high yield, disease-resistant, high-quality.
Technical scheme of the present invention is as follows:
This method is to utilize natural male sterile marigold with pigment material to cultivate male sterile amphitypy system, is that male sterile plants is made female parent with this, with hybridization between selfed lines, makes F
1For hybrid seed.
The breeding method of above-mentioned male sterile amphitypy system is: manually authorize natural male sterile flower with the pollen that homophyletic can be educated, its offspring occurs 50% and can educate, and 50% is sterile, selects the educated strain consistent with maternal character to give sterile strain pollination, continuous 5 generation brother and sister hand over, and cultivate male sterile amphitypy system.
The seed selection of above-mentioned inbred line: select for use the band objective trait to show individual plant preferably aborning, isolate and to breed or bagging selfing 2-3 generation, breed inbred line, in hybridization, make male parent.
Above-mentioned F
1The preparation of hybrid: be that 50% male sterile plants is maternal in being with above-mentioned amphitypy, same hybridization between selfed lines, the plantation male sterile plants is gone than being 1: 1 with inbred line, in 1000 meters isolated areas, spontaneous pollination, but the calm artificial supplementary pollination of pollination phase; The seed of producing in the sterile strain promptly is F
1For hybrid seed, produce use for the land for growing field crops.
The invention has the advantages that: 1, seed production efficiency height, test through the 2004-2005 production of hybrid seeds, the male sterile plant of this amphitypy system is better with a plurality of inbred line coordinate force, the spontaneous pollination ripening rate reaches 70-80%, amount to per mu yield 15-20 kilogram, save artificial 60 than artificial pollination for every mu, be fit to the large tracts of land production of hybrid seeds.2, hybrid vigour is obvious, F
1For the crossbreed increase yield significantly, through test, F
1For 3300 kilograms of fresh flower per mu yields, 2050 kilograms of volume increase 56% of the per mu yield of the calipers kind of comparison photograph.3, according to breeding objective, make the combination of a plurality of merits, the variety resistance shape that this method brings out is outstanding, but the time of infertility no pesticide grown, both saved cost, the pigment that this generation is produced does not have residue of pesticide, improves the quality of pigment; And pigment content increases substantially, and after testing, uses F
1Reach 24.79 ‰ for the fresh flower lutein content, comparison more solito kind contains xanthophyll 20 ‰ and improves 23%.
Embodiment
The breeding method of male sterile amphitypy system is: manually authorize natural male sterile flower with the pollen that homophyletic can be educated, its offspring occurs 50% and can educate, 50% is sterile, select the educated strain consistent with maternal plant height to give sterile strain pollination, continuous 5 generation brother and sister hand over and cultivate that proterties is stable, the male sterile amphitypy of neat and consistent system; Since calendar year 2001, different year, the offspring's that Various Seasonal brother and sister hand over sterile strain all is stabilized in 1: 1 with the segregation ratio that can educate strain, illustrates that this male sterile amphitypy line fertility is relatively stable.
The seed selection of inbred line: select for use and show individual plant preferably aborning, the variant that reaches " scarlet " as kind " scarlet " is spent big, isolates respectively and breeds for 3 generations, breeds inbred line, makes male parent in hybridization.
Male sterile amphitypy system breeds: in 1000 meters isolated areas or protection ground, the male sterile amphitypy is that annual brother and sister hand over breeding.
F
1The preparation of hybrid: be that male sterile plants is maternal in being with amphitypy, same hybridization between selfed lines, the plantation male sterile plants is gone than being 1: 1 with inbred line, in 1000 meters isolated areas, spontaneous pollination, but the calm artificial supplementary pollination of pollination phase; The seed of producing in the sterile strain promptly is F
1For hybrid seed, the flower of sterile strain does not have corolla, can distinguish with educating strain when seed collection yet, gathers respectively, and the seed of producing in the sterile strain is produced for the land for growing field crops and used.
Claims (4)
1, a kind of double-line hybrid breeding method for marigold with pigment is characterized in that: this method is to utilize natural male sterile marigold with pigment material to cultivate male sterile amphitypy system, is that male sterile plants is made female parent with this, with hybridization between selfed lines, makes F
1For hybrid seed.
2, double-line hybrid breeding method for marigold with pigment according to claim 1, it is characterized in that: the breeding method of its male sterile amphitypy system is: manually authorize natural male sterile flower with the pollen that homophyletic can be educated, its offspring occurs 50% and can educate, 50% is sterile, select the educated strain consistent to give sterile strain pollination with maternal character, continuous 5 generation brother and sister hand over, and cultivate male sterile amphitypy system.
3, double-line hybrid breeding method for marigold with pigment according to claim 1, it is characterized in that: the selection of its inbred line is: select for use the band objective trait to show individual plant preferably aborning, isolate and to breed or bagging selfing 2-3 generation, breed inbred line, in hybridization, make male parent.
4, double-line hybrid breeding method for marigold with pigment according to claim 1 is characterized in that: its F
1The compound method of hybrid is: be 50% male sterile plants is maternal in being with above-mentioned amphitypy, and same hybridization between selfed lines, the plantation male sterile plants is gone than being 1: 1 with inbred line, in 1000 meters isolated areas, spontaneous pollination, but the calm artificial supplementary pollination of pollination phase; The seed of producing in the sterile strain promptly is F
1For hybrid seed, produce use for the land for growing field crops.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610059056 CN1817103A (en) | 2006-02-28 | 2006-02-28 | Double-line hybrid breeding method for marigold with pigment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610059056 CN1817103A (en) | 2006-02-28 | 2006-02-28 | Double-line hybrid breeding method for marigold with pigment |
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| Publication Number | Publication Date |
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| CN1817103A true CN1817103A (en) | 2006-08-16 |
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|---|---|---|---|
| CN 200610059056 Pending CN1817103A (en) | 2006-02-28 | 2006-02-28 | Double-line hybrid breeding method for marigold with pigment |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107846858A (en) * | 2015-02-17 | 2018-03-27 | 凯敏工业公司 | Referred to as KI4662 marigold male inbred strain |
| CN107846862A (en) * | 2014-12-16 | 2018-03-27 | 凯敏工业公司 | Entitled KI4537 marigold inbred strais |
| CN115812588A (en) * | 2022-11-10 | 2023-03-21 | 北京市园林绿化科学研究院 | Method for improving female parent setting rate of marigold male sterile line |
-
2006
- 2006-02-28 CN CN 200610059056 patent/CN1817103A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107846862A (en) * | 2014-12-16 | 2018-03-27 | 凯敏工业公司 | Entitled KI4537 marigold inbred strais |
| CN107846862B (en) * | 2014-12-16 | 2021-02-02 | 凯敏工业公司 | Marigold inbred line named KI4537 |
| CN107846858A (en) * | 2015-02-17 | 2018-03-27 | 凯敏工业公司 | Referred to as KI4662 marigold male inbred strain |
| CN107846858B (en) * | 2015-02-17 | 2019-06-21 | 凯敏工业公司 | The marigold male inbred strain of referred to as KI4662 |
| CN115812588A (en) * | 2022-11-10 | 2023-03-21 | 北京市园林绿化科学研究院 | Method for improving female parent setting rate of marigold male sterile line |
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