CN102499060A - Transgenic helicoverpa armigera-resistant and phenol-free cotton breeding method - Google Patents
Transgenic helicoverpa armigera-resistant and phenol-free cotton breeding method Download PDFInfo
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Abstract
The invention relates to a transgenic helicoverpa armigera-resistant and phenol-free cotton breeding method, which belongs to the cotton breeding field, and is characterized in that: eglandular high-yield low-phenol cotton specimen Hunan cotton No.18 with the gene model as Gl2eGl2e as a female parent to be hybrid with a male parent which is high-energetic fiber helicoverpa armigera-resistant specimen Jiangsu 76149. Due to the adoption of the method, the high energetic fibers can be quickly polymerized, high yield can be obtained, disease resistance can be realized, transgenic Bt insect resistance can be realized, a dominant phenol-free property can be realized, the selection efficiency can be remarkably improved, and the breeding period can be shortened. The new bred cotton specimen not only has stable resistance to the helicoverpa armigera and mice so as to reduce the application of helicoverpa armigera control medicine for 2 to 3 times, but also can reduce the production cost, and the cotton seed is safe and reliable to store and transport; and since no cotton phenol and similar substance are contained, the seeds of the cotton can be used as raw materials for processing human edible oil, food and healthcare products, so economic income of the cotton farmers can be increased for 1750 Yuan per Mu, and a promising popularization and application prospect in the cotton production region in China can be realized.
Description
Technical field
The present invention relates to a kind of transgenic bollworm resisting and plant the breeding method that benevolence does not have the phenol cotton, belong to the application of cotton genetic improvement and biotechnology.
Background technology:
Cotton is important fiber crop and economic crops.In results product unginned cotton, gined cotton accounts for 40%, plant benevolence accounts for 35%, plants that shell accounts for 20%, short flannel accounts for 5%.Plant benevolence except that containing the 30%-35% vegetable fat, also contain the 40%-43% high-quality protein.Plant in the cotton benevolence powder after benevolence is extracted oil; Protein content is up to more than 60%; And be rich in 8 seed amino acids of needed by human; Its protein content and trophic component surpass seeds such as paddy rice, wheat, corn and peanut, therefore plant benevolence and are not only important vegetable oil material source, and be the plant protein resource that is only second to soybean in the world.Yet; The cotton variety of China and even global commerial growing mainly is to have phenol cotton at present; Gossypol in its plant body has biochemical resistance to cotton pest; Plant simultaneously and contain 1% gossypol (gossypol) and derivative thereof in the benevolence, can damage its reproduction, blood vessel and nervous system after people and non-ruminant animal are edible, cause intoxicating phenomenons such as male no sperm, female miscarriage.Low-phenol cotton (low gossypol cotton) is the germplasm resource for cotton of non-pigment body of gland among a kind of plant organ, and the gossypol content in its kind benevolence is lower than the food sanitation standard (0.04%) and the national standard (0.02%) of FAO's regulation.Low-phenol cotton kind benevolence is direct-edible or feeding, can produce high-quality edible oil with its oil expression, need not refining, helps the comprehensive utilization of cotton seed.Up to now, successively identify gl both at home and abroad
1, gl
2, gl
3, gl
4, gl
5, gl
6Form relevant gene loci Deng 6 with body of gland, but only gl is arranged
2Gl
3And Gl
2 eThese two kinds of homozygous genotypes show as plant does not have body of gland, plant the low phenol of benevolence.Nineteen fifty-nine U.S. McMichael breeds by double recessive gene (gl from like word filial generation cotton and gined cotton suddenly first
2Gl
3) control no body of gland cotton variety 23B, its plant and seed do not have body of gland, the kind benevolence can directly eat.Subsequently the cotton state of world's main product with this no body of gland kind as backbone parent, through with the hybridization of improved seeds or backcross and carry out the low-phenol cotton breeding.The no body of gland proterties of tradition low phenol cotton variety is by two couples of recessive gene (gl
2Gl
3) control, receive the cotton pollen contamination of phenol easily, thereby cause the degeneration that mixes of low phenol cotton variety.Egyptian scholar Afifi in 1966 etc. pass through
32The low phenol sea-island cotton kind Bahtim 110 of first dominance is cultivated in p mutagenesis, becomes the important germ plasm resource of global dominance low-phenol cotton flower breeding of new variety.Genetic research shows that Bahtim 110 no body of gland proterties receive 1 couple of incomplete dominant gene Gl
2 eControl.States such as Egypt, the U.S. and China hybridize, backcross Bahtim 110 dominant non-gland proterties transformations in upland cotton through upland cotton and sea-island cotton subsequently, breed the upland cotton kind (being) of the low phenol of a series of dominance.
Up to now, the seed selection of low-phenol cotton new varieties and use existing more than 50 year history, yet low-phenol cotton apply the formation scale of always failing.Trace it to its cause and be: (one) low phenol cotton variety lint yield level is lower, and general have a phenol kind underproduction about 5% than popularization at that time; (2) cotton plants non-pigment body of gland lacks the resistance to cotton-plant pest-insects; (3) the low phenol proterties of double recessive is prone to receive the pollution of phenol kind pollen, plants the edible safety of benevolence and is on the hazard; (4) low-phenol cotton unginned cotton phenol content is low, and mouse harm seriously causes the breeding and preservation difficulty of seed; (5) oil of low-phenol cotton kind benevolence is failed exploitation with, edible function, and the industrial chain of its comprehensive utilization remains to be cultivated.Yet shortage high yield, dominance do not have phenol, pest-resistant cultivar is to cause low-phenol cotton not have the one of the main reasons of the market competitiveness.Breeding of the foundation of cotton pyramiding breeding technology and high yield, the low phenol upland cotton kind (being) of dominance and high-intensity fiber, soil of transgenic Bt cotton kind (being) is for seed selection high-quality, high yield, pest-resistant, no phenol new cotton variety provide technical support and breeding parent.Jiangsu is economically well-developed province, of flowing rhythm along with unginned cotton's purchasing price over past ten years, and sown areas of cotton constantly reduce, and plant low recovery and the development that is seriously restricting cotton production of cotton comparative effectiveness.Jiangsu is again the big province of textile industry, and the whole province's cotton textiles industry production scale in 2007 and yarn, cotton output all occupy the big front three of economizing of China's weaving.Since two thousand four; Jiangsu Province's annual weaving amount of cotton is stabilized in more than 700,000 tons, calculates the non-weaving amount of cotton according to 7% of aggregate demand, and the annual requirement of the whole province's raw cotton is stabilized in more than 750,000 tons; Yet the same period, the whole province's gined cotton gross output only reached about 350,000 tons, and insufficiency of supply-demand reaches more than 400,000 tons.Seed selection and popularizing planting high yield, pest-resistant, no phenol new cotton variety; Edible oil, food and health products function through the no phenol cotton seed benevolence of exploitation; Increase significantly and plant cotton benefit, thereby improve cotton grower's the cotton enthusiasm of planting, guarantee the supply safety of China's grain, raw cotton and edible oil.Therefore, a kind of anti insect gene of Bt efficiently of invention, Gl
2 eThe low phenolic group of dominance because of transformation, evaluation and system of selection, pest-resistant, the low phenol of polymerization, high yield, high-intensity fiber, disease-resistant gene are aims of the present invention in one.
Summary of the invention
Technical problem
The objective of the invention is to: to low phenol kind in the cotton production practice be prone to receive the phenol kind pollen contamination, lack resistance problem to cotton pest and mouse; Through the genetic improvement approach; Adopt the assisted Selection of sexual hybridization and incorporation of markings proterties; Polymerization is pest-resistant, low phenol, high yield, high-intensity fiber, merit such as disease-resistant, cultivates transgenic bollworm resisting, dominance low-phenol cotton flower new varieties, and the oil of development and use low-phenol cotton kind benevolence is with, edible function; Improve and to plant cotton economic benefit, for the cotton successful transformation is that grain, cotton, oily dual-purpose type crops provide kind to support.
Technical scheme
Content of the present invention is a kind of transgenic bollworm resisting, plants the breeding method that benevolence does not have the phenol cotton, it is characterized in that:
1) selecting the non-gland gene type for use is Gl
2 eGl
2 eHigh yield low-phenol cotton flower variety Hunan be that maternal (♀) is male parent with high-tenacity fiber, bollworm resisting strain Soviet Union 76149 cotton No. 18
The hybridization combo;
2) plantation F
1Colony, whole individual plants of maturing stage mix gather in the crops F
2
3) plantation F
2, F
3, F
4, colony's planting scale is greater than 300 strains, and pulled out body of gland cotton seedling through the surface has, the evaluation of non-pigment body of gland to cotton seedling organ (stem, cotyledon, true leaf and petiole) tri-leaf period, selects the cotton seedling of no body of gland; Six leaf phases are surperficial with writing brush spot printing 2000ppm kanamycin solution at the tender true leaf of children; Observe the blade face after one week and yellowish-white spot whether occurs; Eliminate the sense kanamycin plant that presents the chlorosis spot, keep the anti-kanamycin plant of the green of acting normally, receipts are mixed in ripe back all individual plant;
4) under planting density 36,000 strains/hectare condition, F
5Begin to select that boll per plant is several more than 25, average bell is heavy>=5.2 grams, the no body of gland individual plant of ginning outturn>=39%, anti-kanamycin;
5) plantation F
6-F
7Each system of strain from generation to generation, with the sense worm phenol kind Soviet Union being arranged is contrast cotton No. 22:
Indoor bollworm resistance is identified.
In view of field resistance is identified the influence that is subject to environmental condition, resistance to insects is identified and is taked indoor young leaflet tablet to connect the worm method., each adopts back 20 stem tender leafs (every strain is adopted 1 at most) in waiting to reflect strain system and check variety colony at random in cotton bud stage; Every culture dish is put 2 tender leafs; Packing tightly petiole base with absorbent cotton preserves moisture; In each culture dish, connect 6 of identical cotton bollworm low instar larvaes in the length of time, observe the dead borer population of statistics behind the 72h, the borer population of living, live the worm length of time and the killed degree of blade etc.
With reference to the standard of formulations in 1997 such as Shu Chune, the killed degree of blade is divided into 3 grades, the I level: being injured, it is not in blocks to be the syringe needle shape; II level: be injured into small pieces, be no more than 1/4 of leaf area; The III level: killed in blocks, surpass 1/4 of leaf area.
Resistance to insects is divided into 3 grades, high anti-: larva corrected mortality >=80%, the killed one-tenth of blade I level; In anti-: larva corrected mortality 60%-79%, the killed one-tenth of blade II level; Low anti-: larva corrected mortality＜59%, the killed one-tenth of blade III level.In the mensuration, if control group larval mortality＞20% then must redeterminate.
Select the resistance grade to reach the strain system that height is anti-, economical character is stable.
Planting the benevolence gossypol content detects.
Maturing stage results plant does not have body of gland, the high resistance helicoverpa armigera strain is the unginned cotton, dries back cotton ginning acquisition cotton seed naturally.Adopt clamp separation cotton seed hull and plant benevolence, (standard GB/T13086-1991) the kind benevolence free gossypol content of roguing system and check variety is gone in detection to adopt " assay method of free gossypol in the feed ".Select gossypol content to be lower than the strain system of the cotton threshold value 0.0004% of no phenol, be the transgenic bollworm resisting that is obtained, the strain that kind benevolence does not have the phenol cotton.
In conjunction with land for growing field crops cotton bollworm abiogenesis resistance screening result, under planting density 36,000 strains/hectare condition, F
6Beginning is only at gland gene type Gl
2 eGl
2 e, high resistance helicoverpa armigera several more than 25, the average bell of cell selecting boll per plant heavy>=5.2 grams, ginning outturn>=39%; And the individual plant of fibre length>=29mm, specific strength>=28c N/tex, mic value 3.7-4.8; Obtain pest-resistant, plant benevolence and do not have phenol new lines " Su Yan 606 ", its plant height 95-100cm, boll per plant count 25.8-27.2; The heavy 5.4-5.6 gram of bell, ginning outturn 40.77%; Fibre length 29.70mm, specific strength 30.6c N/tex, mic value 4.5; Plant benevolence free gossypol content 0.000197%, reach the cotton standard of no phenol.
Beneficial effect
Advantage of the present invention and effect are:
Adopt high yield, dominance low-phenol cotton flower variety Hunan cotton No. 18 as one of parent, pest-resistant, the no phenol new cotton variety of breeding, its kind benevolence does not have the phenol proterties and receives 1 couple of dominant gene Gl
2 eControl.Cotton belongs to entomophilous flower, 2 couples of recessive gene gl
2Gl
3The low phenol cotton variety of control, if insects such as honeybee will have phenol kind pollen to take on the low phenol kind column cap in flowering stage, contemporary a part of cotton seed kind benevolence performance has phenol, the edible safety of planting benevolence is affected, and loses value immediately.And 1 couple of dominant gene Gl
2 eEven the low phenol cotton variety of control receives the influence of honeybee natural pollination, contemporary cotton seed kind benevolence still shows no phenol, has overcome the drawback that the double recessive low phenol cotton variety is subject to biology hybrid.
The present invention is at the low generation (F of breeding
2～F
4) in the colony, adopt with the trans Bt gene bollworm resisting respectively, plant benevolence and do not have the anti-kanamycin of the closely-related mark property of phenol, plant and do not have body of gland and select indirectly, in conjunction with south numerous added-generation, can improve efficiency of selection, shortening the breeding cycle significantly.And at the advanced lines (F of breeding
6～F
7) in the colony, two objective trait trans Bt gene bollworm resistings that genotype has been isozygotied, plant benevolence and do not have phenol and directly carry out character test, can concentrate one's energy that a spot of strain system is carried out selfing and isozygoty, identify and selects, the thing of realization breeding effect is partly and merit times.
The new varieties polymerization that the inventive method is bred hybrid strain both sides' high-intensity fiber, high yield, bollworm resisting, kind benevolence do not have phenol, characteristic such as disease-resistant; Through no phenol seed kernel oil with, the minimizing of edible and function development for health care and agricultural chemicals usage amount; Can improve significantly and plant cotton economic benefit; Promote agricultural produce, increasing peasant income, have broad application prospects in vast cotton region.
Description of drawings
Fig. 1 harvest time pest-resistant, no phenol new lines Su Yan 606 (right sides) and sense worm, have phenol kind Soviet Union cotton No. 22 (left sides) to plant that pigment gland (stain) distributes relatively in Electronic Speculum visual field, benevolence vertical section.
Fig. 2 Su Yan 606 seed selection processes
Embodiment
Select the parent
Cotton No. 18 of Hunan [Zhang Xuelin, Jin Lin, Ceng Zhaoyun etc. the seed selection preliminary study that Hunan is cotton No. 18, Chinese cotton, 2000,27 (10): 18-19], high yield, dominance low-phenol cotton flower variety, Hunan Province's Cotton Research Institute nineteen ninety-five is bred, and names through Hunan Province's variety certification in 2000.The 123 days time of infertility of this kind, under planting density 36,000 strains/hectare condition, plant height 100-105cm, boll per plant 24.5-26.2, single bell weighs 5.7 grams, ginning outturn 42.48%.Fiber upper half mean length 28.3mm, fracture specific strength 29.3cN/tex, mic value 5.1.Plant benevolence free gossypol content 0.00304%, be lower than national food sanitation standard 0.02%, belong to the low-phenol cotton type.
Soviet Union 76149 [Wu Qiaojuan, Xiao Songhua, Liu Jianguang etc. the cultivation of high-quality pest-resistant cotton new germ plasm; The Jiangsu agricultural science; 2008 (6): 42-44], high-tenacity fiber, bollworm resisting cotton strain, Commercial Crop Inst., Jiangsu Prov. Academy of Agricultural Sciences bred and named in 2004.The 135 days time of infertility of this strain, under planting density 36,000 strains/hectare condition, plant height 95-100cm, boll per plant 21.6-23.8, single bell weighs 6.4 grams, ginning outturn 34.89%.Fiber upper half mean length 32.6mm, fracture specific strength 40.0cN/tex, mic value 4.2.Cotton bollworm larvae corrected mortality 86.05%, the killed one-tenth of blade I level, high resistance helicoverpa armigera.Plant benevolence free gossypol content 0.35608%, be higher than national food sanitation standard 0.02%, genus has the cotton type of phenol.
Breed breeding
Selected for use high yield, cotton No. 18 the high-tenacity fibers in low-phenol cotton flower variety Hunan, bollworm resisting strain Soviet Union 76149 to be male parent
hybridization combo in summer in 2005 for maternal (♀) and self-fertile in Nanjing.Plant F in The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute wild cotton plantation in Sanya, Hainan Province winter then
1Spring in 2006 in Nanjing, then winter in the Sanya, spring in 2007 planted F respectively in Nanjing
2, F
3, F
4, population size is greater than 300 strains, and pulled out body of gland cotton seedling through the surface has, the evaluation of non-pigment body of gland to cotton seedling organ (stem, cotyledon, true leaf and petiole) tri-leaf period, selects the cotton seedling of no body of gland; Six leaf phases are surperficial with writing brush spot printing 2000ppm kanamycin solution at the tender true leaf of children; Observe the blade face after one week and yellowish-white spot whether occurs; Eliminate the sense kanamycin plant that presents the chlorosis spot, keep the anti-kanamycin plant of the green of acting normally, receipts are mixed in ripe back all individual plant.Winter in 2007 was planted F in the Sanya
5, more than population size 300 strains, under planting density 36,000 strains/hectare condition, select that boll per plant is several more than 25, average bell is heavy the maturing stage>=5.2 grams, 56 of the no body of gland individual plants of ginning outturn>=39%, anti-kanamycin; Individual plant gined cotton samples using HVI900 series fiber quality analyzer is measured the fiber quality index; According to the requirement of fibre length>=29mm, specific strength>=28c N/tex, mic value 3.7-4.8, final election obtains 15 high-intensity fibers, high yield, anti-kanamycin, no body of gland individual plant.Spring in 2008 is in Nanjing, plant F winter respectively then in the Sanya
6-F
7Strain system, and to feel worm, cotton No. 22 of phenol kind Soviet Union (Soviet Union examines cotton 200204) to be arranged as contrast.
Indoor bollworm resistance is identified: resistance to insects is identified and is taked indoor young leaflet tablet to connect the worm method;, each adopts back 20 stem tender leafs in cotton bud stage at random in waiting to reflect strain system and check variety colony; Every strain is adopted 1 at most, and every culture dish is put 2 tender leafs, packs tightly petiole base with absorbent cotton and preserves moisture; In each culture dish, connect 6 of identical cotton bollworm low instar larvaes in the length of time, observe the dead borer population of statistics behind the 72h, the borer population of living, live the worm length of time and the killed degree of blade; With reference to the standard of formulations in 1997 such as Shu Chune, the killed degree of blade is divided into 3 grades, the I level: being injured, it is not in blocks to be the syringe needle shape; II level: be injured into small pieces, be no more than 1/4 of leaf area; The III level: killed in blocks, surpass 1/4 of leaf area; Resistance to insects is divided into 3 grades, high anti-: larva corrected mortality >=80%, the killed one-tenth of blade I level; In anti-: larva corrected mortality 60%-79%, the killed one-tenth of blade II level; Low anti-: larva corrected mortality＜59%, the killed one-tenth of blade III level, in the mensuration, if control group larval mortality＞20% then must redeterminate, selection resistance grade reaches high anti-strain and is:
Planting the benevolence gossypol content detects: it is the unginned cotton that the plant of maturing stage results high resistance helicoverpa armigera does not have the body of gland strain; Naturally dry the back cotton ginning and obtain cotton seed; Adopt clamp to separate cotton seed hull and kind benevolence; Adopt standard GB/T13086-1991 " assay method of free gossypol in the feed " to detect the kind benevolence free gossypol content of roguing system and check variety, select gossypol content to be lower than the strain system of the cotton threshold value 0.0004% of no phenol, acquisition transgenic bollworm resisting, kind benevolence do not have 6 of the new lines of phenol cotton.
Spring in 2009 served as to supply the examination material cotton No. 22 with pest-resistant, no phenol new lines and Soviet Union; The multiple spot test is carried out in Xu Huai, coastal, riverine cotton region in Jiangsu Province; Through to output, fiber quality, pest-resistant, disease-resistant and plant the evaluation of proterties such as benevolence gossypol content, obtain high-intensity fiber, high yield, pest-resistant, no phenol cotton excellent strain Su Yan 606.
Transgenic bollworm resisting, kind benevolence through above method is bred do not have phenol cotton new lines " Su Yan 606 ", cotton bollworm larvae corrected mortality 89.67%, the killed one-tenth of blade I level, high resistance helicoverpa armigera.Plant benevolence gossypol content 0.000197%,, reach the cotton standard of no phenol far below national food sanitation standard 0.02%.Under planting density 36,000 strains/hectare condition, plant height 95-100cm, boll per plant count 25.8-27.2, the heavy 5.4-5.6 gram of bell, ginning outturn 40.77%; Fiber upper half mean length 29.70mm, fracture specific strength 30.6c N/tex, mic value 4.5.
Claims (2)
- A transgenic bollworm resisting with plant benevolence and do not have the breeding method of phenol cotton, it is characterized in that:1) selecting the non-gland gene type for use is Gl 2 eGl 2 eHigh yield, low-phenol cotton flower variety Hunan be maternal ♀ cotton No. 18, reviving with high-tenacity fiber, bollworm resisting strain 76149 is male parentThe hybridization combo;
2) plantation F 1Colony, whole individual plants of maturing stage mix gather in the crops F 23) plantation F 2, F 3, F 4, colony's planting scale is greater than 300 strains, and pulled out body of gland cotton seedling through to cotton seedling organ stem, cotyledon, true leaf with the petiole surface has, the evaluation of non-pigment body of gland tri-leaf period, selects no body of gland cotton seedling; Six leaf phases are surperficial with writing brush spot printing 2000ppm kanamycin solution at the tender true leaf of children; Observe the blade face after one week and yellowish-white spot whether occurs; Eliminate the sense kanamycin plant that presents the chlorosis spot, keep the anti-kanamycin plant of the green of acting normally, receipts are mixed in ripe back all individual plant;4) under planting density 36,000 strains/hectare condition, F 5Select that boll per plant is several more than 25, average bell is heavy>=5.2 grams, the no body of gland individual plant of ginning outturn>=39%, anti-kanamycin;5) plantation F 6-F 7Each system of strain from generation to generation, with the sense worm phenol kind Soviet Union being arranged is contrast cotton No. 22:Indoor bollworm resistance is identified:Resistance to insects is identified and is taked indoor young leaflet tablet to connect the worm method;, each adopts back 20 stem tender leafs in cotton bud stage at random in waiting to reflect strain system and check variety colony; Every strain is adopted 1 at most, and every culture dish is put 2 tender leafs, packs tightly petiole base with absorbent cotton and preserves moisture; In each culture dish, connect 6 of identical cotton bollworm low instar larvaes in the length of time, observe the dead borer population of statistics behind the 72h, the borer population of living, live the worm length of time and the killed degree of blade;With reference to the standard of formulations in 1997 such as Shu Chune, the killed degree of blade is divided into 3 grades, the I level: being injured, it is not in blocks to be the syringe needle shape; II level: be injured into small pieces, be no more than 1/4 of leaf area; The III level: killed in blocks, surpass 1/4 of leaf area;Resistance to insects is divided into 3 grades, high anti-: larva corrected mortality >=80%, the killed one-tenth of blade I level; In anti-: larva corrected mortality 60%-79%, the killed one-tenth of blade II level; Low anti-: larva corrected mortality＜59%, the killed one-tenth of blade III level:Select the resistance grade to reach the high strain system that resists;Planting the benevolence gossypol content detects:Maturing stage results plant does not have body of gland, the high resistance helicoverpa armigera strain is the unginned cotton; Obtain cotton seed; Adopt standard GB/T13086-1991 " assay method of free gossypol in the feed " to detect roguing system kind of benevolence free gossypol content; Select gossypol content to be lower than the strain system of the cotton threshold value 0.0004% of no phenol, be the transgenic bollworm resisting that is obtained, the strain that kind benevolence does not have the phenol cotton. - 2. method according to claim 1 is characterized in that:Under planting density 36,000 strains/hectare condition, F 5Select simultaneously that the knot bell is several more than 25, average bell is heavy>=5.2 grams, the no body of gland individual plant of ginning outturn>=39%, fibre length>=29mm, specific strength>=28c N/tex, mic value 3.7-4.8, anti-kanamycin.
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| CN103493724A (en) * | 2013-08-28 | 2014-01-08 | 山东农兴种业有限责任公司 | Seed selection and hybrid seed production method of pest-resistant, low-gossypol and stigma-exposed bilinear hybrid cotton |
| CN103493724B (en) * | 2013-08-28 | 2016-04-27 | 山东农兴种业有限责任公司 | The cotton seed selection of a kind of pest-resistant low phenol stigma appearing double-line hybrid and cross-breeding method |
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| CN103884848B (en) * | 2014-04-02 | 2015-08-05 | 江苏省农业科学院 | A kind of method predicting transgenic Bt cotton insect resistace intensity |
| CN104719122A (en) * | 2015-02-11 | 2015-06-24 | 国营全椒县棉花原种总场 | Culture method of transgenic insect-resistant hybrid cotton |
| CN105494079A (en) * | 2015-12-02 | 2016-04-20 | 中国农业科学院棉花研究所 | Method for acquiring cotton plants with high insect resistance |
| CN105706914A (en) * | 2016-04-01 | 2016-06-29 | 山西农业大学 | New germplasm characterized by delayed-formation of cotyledons pigment glands of cotton and breeding method of new germplasm |
| CN112167054A (en) * | 2020-10-21 | 2021-01-05 | 湖南省棉花科学研究所 | Breeding method of new transgenic high-quality upland cotton variety |
| CN112167054B (en) * | 2020-10-21 | 2022-07-01 | 湖南省棉花科学研究所 | Breeding method of new transgenic high-quality upland cotton variety |
| CN113207681A (en) * | 2021-05-31 | 2021-08-06 | 河北省农林科学院棉花研究所（河北省农林科学院特种经济作物研究所） | Breeding method of recessive glandless cotton |
| CN114532175A (en) * | 2022-02-18 | 2022-05-27 | 新疆农垦科学院 | Method for identifying physiological aphid resistance based on cotton leaf downy characteristics |
| CN114532175B (en) * | 2022-02-18 | 2023-10-27 | 新疆农垦科学院 | Identification method for physiological aphid resistance based on fuzz characteristics of cotton leaves |
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