CN104719122A - Culture method of transgenic insect-resistant hybrid cotton - Google Patents
Culture method of transgenic insect-resistant hybrid cotton Download PDFInfo
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- CN104719122A CN104719122A CN201510073381.0A CN201510073381A CN104719122A CN 104719122 A CN104719122 A CN 104719122A CN 201510073381 A CN201510073381 A CN 201510073381A CN 104719122 A CN104719122 A CN 104719122A
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Abstract
The invention relates to the technical field of planting industry, and discloses a culture method of transgenic insect-resistant hybrid cotton. The method comprises the following steps: culturing a female parent material integrating high yield and disease resistance properties; culturing a male parent material integrating the insect resistance and disease resistance properties; preparing a hybridized combination; obtaining the heterosis; performing purification and reproduction for parents. With the adoption of the method, the cultured transgenic insect-resistant hybrid cotton has comprehensive outstanding properties, such as high yield, high quality, disease resistance and insect resistance; the purification and reproduction are performed for parents, so that the heterosis can be remained continuously; the prepared hybrid cotton is high in purity and long in production and application time.
Description
Technical field
The present invention relates to plant husbandry technical field, refer more particularly to a kind of breeding method of Insect resistant crosses.
Background technology
The research of China's transgene cotton starts from early 1990s.In anti insect gene research, succeed in developing China's first GFM Cry I A killing gene, and this channel genes cotton is obtained the new cotton variety " anti-cotton No. 1 of state " of high resistance helicoverpa armigera, " anti-cotton No. 12 of state ", " cotton No. 26 of Shanxi " etc., China become independently succeed in developing Insect Resistant Cotton in the world and have second country of independent intellectual property right.In order to prevent the lepidoptera pests such as cotton bollworm, resistance is produced to unit price Bt gene cotton, China takes the lead in again succeeding in developing Cry I A+CpTI divalent insect-resistant gene, import bivalent transgenic pest-resistant cotton new varieties " SGK321 ", " CCRI 41 ", " CCRI 45 " etc. that cotton obtains bollworm resisting, technical merit is leading in the world.
New cotton variety transgenosis is the main trend of cotton development both at home and abroad in recent years.According to the study, the entirety of the cotton new varieties of trans-exogenous gene pest-resistant is pest-resistant stable lasting, and pest-resistant spectrum is also comparatively wide, in Insect Resistant Cotton is applied, have clear superiority.Therefore, the Extrinsic Anti-insect Genes of importing is by unit price gene to bivalent gene even multivalent genetic future development, and resistance to multiple resistance future development, just becomes inevitable development trend by single resistance.
Cotton hybrid vigor is strong, is hybridized by Parent, realizes parents and has complementary advantages.Hybrid cotton is higher in China's popularity, and wherein cotton region, the Yangtze river basin is the maximum high cotton growing area of China's hybrid cotton popularizing area, and hybrid cotton cultivated area accounts for more than 95% of this cotton region; The hybrid cotton cultivated area in cotton region, the Huanghe valley accounts for more than 50% of this cotton region; Along with the popularization of cotton seed sowing seeds technology, Xinjiang cotton is also just spread plantation hybrid cotton.
The yielding ability of foreign gene resistance to insects and hybrid cotton, quality are incorporated into one by Insect resistant crosses, and comprehensive advantage is obvious, and development potentiality is huge, has a extensive future.
" Q2088 " is Insect resistant crosses, and the combination properties such as its yielding ability, quality, resistance to insects are given prominence to, and promotes " Q2088 " great to promotion Cotton in China production Significance of Sustainable Development.
Summary of the invention
The object of the present invention is to provide a kind of breeding method of Insect resistant crosses, the hybrid cotton of cultivation is polymerized good yielding ability and quality and stronger disease resistance and resistance to insects, yield stability is good, wide adaptability.
For achieving the above object, the present invention is by the following technical solutions:
A breeding method for Insect resistant crosses, comprises following sequential steps:
(1) female parent material of polymerization high yield and disease resistance trait is cultivated;
(2) male parent material of being polymerized pest-resistant and disease resistance trait is cultivated;
(3) step (1) female parent material, step (2) male parent material preparing hybrid is utilized to combine;
(4) parent's pure keeping and breeding.
Further, the female parent stating step (1) is that directive breeding forms from " the cotton No. 4 × CCRI 12 of nasal mucus " offspring.
Further, the male parent of described step (2) is that systematic breeding forms from " 99 × 86-1 " offspring.
Further, described step (3) is that the material above-mentioned steps (1) cultivated is as female parent, material above-mentioned steps (2) cultivated, as male parent, adopts the method preparing hybrid kind of artificial emasculation, thus obtains Insect resistant crosses new varieties.
Further, pure keeping and the propagation technique of described step (4) comprising: select rice belt to breed parental seed, the independent isolation implant of Parent, forbid the cottons such as plant cotton within parent propagation field surrounding 500 meters; Omnidistance examination parental trait, seals colored selfing to the preferred plant with typical proterties, after examining or check the performance of Single boll weight, ginning outturn, fiber quality and the feature such as shape of the seed and size again after point individual plant results, using the parent of Superior line mixing as hybrid seeding.
The present invention compared with prior art, has the following advantages:
(1) to be polymerized the material of high yield and disease resistance trait for female parent, to be polymerized the material of pest-resistant and disease resistance trait for male parent, the Insect resistant crosses cultivated by heterosis utilization has high yield, high-quality, the comprehensively merit such as disease-resistant, pest-resistant;
(2) by parent's pure keeping and breeding, can continue to keep hybrid vigour not fail, the purity of hybrid of preparation is high, long in the production application time.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is further described in detail.
A breeding method for Insect resistant crosses, comprises following sequential steps:
(1) female parent material of polymerization high yield and disease resistance trait is cultivated;
" Q2088 " female parent material code name is " B2638 ", is that cotton original seed resultant field directive breeding from " the cotton No. 4 × CCRI 12 of nasal mucus " offspring in Quanjiao County forms.Wherein " cotton No. 4 of nasal mucus " is the high yield of Siyang, Jiangsu cotton protospecies field seed selection, disease-resistant, high-quality cotton new varieties, and be typical high yield Type Cotton kind, lint percentage reaches 43.3%, and gined cotton per mu yield is than check variety " cotton No. 3 of nasal mucus " volume increase 8.48%; Fiber quality is excellent, fiber upper half mean length (HVICC calibration) 29.23 millimeters, strength 27.93 lis of ox/Tekes, micron value 4.5, regularity 83.73%.But " cotton No. 4 of nasal mucus " disease resistance performance is general.The present invention mainly utilizes its yielding ability.The cotton variety of " CCRI 12 " Cotton Inst., Chinese Agricultural Academy's seed selection, has good double resistance to cotton wilt and verticillium wilt.Through qualification, " CCRI 12 " fusarium wilt index 4.1, reaches high resistance to wilt good level, verticillium wilt index 14.3, reaches resisting verticillium level.
The present invention select " nasal mucus cotton No. 4 " for maternal, " CCRI 12 " hybridize for male parent, filial generation, at Infected Field continuous 5 years M8003 line, is cultivated and had successfully not only got bumper crops but also disease-resistant cotton new material " B2638 ".
(2) male parent material of being polymerized pest-resistant and disease resistance trait is cultivated;
" Q2088 " male parent material code name is " A5688 ", and being Quanjiao County cotton original seed resultant field from " 99 × 86-1 " offspring, directive breeding forms.Wherein " in 99 " is high yield, the pest-resistant cotton strain that Cotton Inst., Chinese Agricultural Academy cultivates, and in 1986-1987 years pest-resistant Combined Trials of national cotton, two annual lint yields are 94.8 kilograms, occupy 16 pest resistant strain first places.
Anti-blight, the high-yield variety that seed selection becomes is presided over by Institute of Plant Protection of " 86-1 " the Chinese Academy of Agricultural Sciences, in cotton breeding, be used as anti-source material more.The present invention mainly utilizes its disease resistance.
(3) preparing hybrid combination, obtains hybrid vigour;
" Q2088 " with not only get bumper crops but also disease-resistant material " B2638 " for maternal, with not only pest-resistant but also disease-resistant material " A5688 " for the combination of male parent preparing hybrid.Adopt artificial emasculation technology to produce crossbreed, thus obtain hybrid vigour.But " Q2088 " hybrid seed production technology has mainly carried out following innovation: one is selection rice belt, hybrid seeding plot and is the area that labour is abundant, can accomplish that nature is isolated, prevent and treat the pollination of other cottons, guaranteed hybrid seed purity; Two is hybrid seeding cotton culture is selected paddy soil plant parent, can alleviate disease, can prevent again lodging, combine technology such as going flower bud morning simultaneously, promotes that female parent is concentrated into bell, improves hybrid seed production yields; Three is adopt complete stool hybridization, guarantees that stripping completes artificial pollination work, to ensure hybrid seeding quality before having spent male nine o'clock of morning next day.
(4) parent's pure keeping and breeding;
For improving parental purity, guarantee that hybridizing all property does not degenerate, the present invention selects rice belt to breed " Q2088 " parental seed, forbids the cottons such as plant cotton within parent propagation field surrounding 500 meters.The independent isolation implant of Parent, seedling stage, squaring period, flowering stage and the term of opening bolls whole process parental trait is examined or check, strict eliminate the individual plant that economical character does not meet parent's standard, reduce the generation of residual variation on genetics.Colored selfing is sealed to the preferred plant that each has parent typical case proterties simultaneously, ripe time-division individual plant results, after the performance of examination Single boll weight, ginning outturn, fiber quality and the feature such as shape of the seed and size, using the parent of preferred individual plant mixing as hybrid seeding.
The present invention adopts above-mentioned technical method to carry out totally 6 generations breeding in 4 years to " Q2088 " Parent, and still can keep parental seed purity more than 99%, and kind property is continued to keep, the crossbreed of preparation still has stronger hybrid vigour.
It should be noted last that, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (5)
1. a breeding method for Insect resistant crosses, is characterized in that, comprises following sequential steps:
(1) female parent material of polymerization high yield and disease resistance trait is cultivated;
(2) male parent material of being polymerized pest-resistant and disease resistance trait is cultivated;
(3) step (1) female parent material, step (2) male parent material preparing hybrid is utilized to combine;
(4) parent's pure keeping and breeding.
2. the breeding method of Insect resistant crosses according to claim 1, is characterized in that, the female parent of described step (1) is that directive breeding forms from " the cotton No. 4 × CCRI 12 of nasal mucus " offspring.
3. the breeding method of Insect resistant crosses according to claim 1, is characterized in that, the male parent of described step (2) is that systematic breeding forms from " 99 × 86-1 " offspring.
4. the breeding method of Insect resistant crosses according to claim 1, it is characterized in that, described step (3) is that the material above-mentioned steps (1) cultivated is as female parent, material above-mentioned steps (2) cultivated is as male parent, adopt the method preparing hybrid kind of artificial emasculation, thus obtain Insect resistant crosses new varieties.
5. the breeding method of Insect resistant crosses according to claim 1, it is characterized in that, the pure keeping of described step (4) and propagation technique select rice belt to breed parental seed, the independent isolation implant of Parent, forbids the cottons such as plant cotton within parent propagation field surrounding 500 meters; Omnidistance examination parental trait, seals colored selfing to the preferred plant with typical proterties, after examining or check the performance of Single boll weight, ginning outturn, fiber quality and the feature such as shape of the seed and size again after point individual plant results, using the parent of Superior line mixing as hybrid seeding.
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Citations (4)
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| CN101427653A (en) * | 2007-11-09 | 2009-05-13 | 中国农业科学院棉花研究所 | Breeding method for pest-resistant green fibre new species of cotton |
| CN102119654A (en) * | 2010-11-29 | 2011-07-13 | 山东冠丰种业科技有限公司 | Method for breeding new variety of insect-resistant cotton |
| CN102499060A (en) * | 2011-11-01 | 2012-06-20 | 江苏省农业科学院 | Transgenic helicoverpa armigera-resistant and phenol-free cotton breeding method |
| CN102948364A (en) * | 2012-11-11 | 2013-03-06 | 潜江市特色作物高科研究所 | High-yield and good-quality insect-resistant cotton hybrid with big cotton bolls and breeding method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101427653A (en) * | 2007-11-09 | 2009-05-13 | 中国农业科学院棉花研究所 | Breeding method for pest-resistant green fibre new species of cotton |
| CN102119654A (en) * | 2010-11-29 | 2011-07-13 | 山东冠丰种业科技有限公司 | Method for breeding new variety of insect-resistant cotton |
| CN102499060A (en) * | 2011-11-01 | 2012-06-20 | 江苏省农业科学院 | Transgenic helicoverpa armigera-resistant and phenol-free cotton breeding method |
| CN102948364A (en) * | 2012-11-11 | 2013-03-06 | 潜江市特色作物高科研究所 | High-yield and good-quality insect-resistant cotton hybrid with big cotton bolls and breeding method thereof |
Non-Patent Citations (2)
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| 王霞等: "转基因杂交棉新品种Q2088的选育及栽培技术", 《现代农业科技》 * |
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