CN106376454B - The selection of anti-nicosulfuron millet germplasm and application - Google Patents
The selection of anti-nicosulfuron millet germplasm and application Download PDFInfo
- Publication number
- CN106376454B CN106376454B CN201610817719.3A CN201610817719A CN106376454B CN 106376454 B CN106376454 B CN 106376454B CN 201610817719 A CN201610817719 A CN 201610817719A CN 106376454 B CN106376454 B CN 106376454B
- Authority
- CN
- China
- Prior art keywords
- nicosulfuron
- millet
- antiweed
- generation
- lines
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The selection of anti-nicosulfuron millet germplasm, belong to the technical field of crossbreeding, using remote edge sexual hybridization, the method breeding being returned again, the following steps are included: (1), using anti-nicosulfuron Green foxtail material as male parent, distant hybridization is carried out to cultivate millet to be maternal, the F1 plant shoots phase sprays nicosulfuron, obtains the hybrid plant with nicosulfuron resistance；(2), hybrid is continued to carry out 2-4 for hybridizing with cultivation millet, selects resistance individual plant selfing 2-3 generation, filtering out Agronomic is millet and the seed with nicosulfuron resistance, obtains anti-nicosulfuron millet new germ plasm.Selection hybridization procedures of the invention simplify, and improve hybridization, are returned successfully accuracy, confidence level.The anti-nicosulfuron millet new germ plasm economical character that method of the invention selects is preferable, has nicosulfuron resistance.
Description
Technical field
The invention belongs to the technical fields of crossbreeding, are related to initiative and the breed breeding of millet new germ plasm, and in particular to
A kind of method for creating of anti-nicosulfuron (Nicosulfuron) millet germplasm and its application and application side in Millet Breeding
Method.Selection of the present invention, breeding is simple, strong operability, and success rate of breeding is high.
Background technique
Millet itself does not have antiweed or Bar gene, but the Wild related germplasm resource of millet is very rich
Richness has extensive hereditary variation type, has great importance (Wang Tianyu etc., 2000) to the antiweed breeding of millet.
For many years, domestic and international researcher successively has found different resist in millet Wild related germplasm Green foxtail natural mutation group
It is more to obtain anti-Atrazine, anti-trefanocide, anti-Sethoxydin, anti-imazethapyr etc. by hybridizing with millet for herbicide resistance gene
Kind of resistance millet variety (Darmency and Pernes, 1985；Darmency and Pernes 1989；Wang Tianyu etc.,
2000；Shi Zhigang etc., 2010).
Nicosulfuron is the sulfonylurea herbicide with efficient, low toxicity, strong selectivity and low dosage, is called Yu Nongle,
Most annual, perennial gramineae weeds and broadleaf weeds and nutgrass flatsedge can be effectively prevented and kill off, it can be with other a variety of herbicides, peace
Full agent is mixed.1996 registration be used for corn, foreign patent (CN87100436) in January, 2007 it is expired (Liu Minghong,
2012).In China, nicosulfuron original agent realizes rapidly localization, and cost is greatly lowered, and yield rises rapidly.It cuts
To in March, 2014, the nicosulfuron product in the term of validity of registration has 383, wherein raw medicine product 42, and formulation products 341,
Compared to other several herbicides, medicine source is very extensive.Therefore, the millet new germ plasm and new varieties of anti-nicosulfuron are cultivated, it is right
Millet production is of great significance.
Summary of the invention
The object of the present invention is to provide a kind of method for creating of anti-nicosulfuron millet germplasm and in Millet Breeding and production
In application method.The present invention successfully formulates out the millet germplasm of anti-nicosulfuron, upper available anti-in production to cultivate
Nicosulfuron herbicide agent Millet Variety is laid a good foundation.Anti- nicosulfuron millet variety is safely and effectively applied in production,
The major transformation that millet will be caused to produce.Manpower and financial resources can be largely saved, intensive system agricultural industry is established, is had
Have wide practical use, huge economic benefit and social benefit will be generated.
The present invention be realize its purpose the technical solution adopted is that:
The selection of anti-nicosulfuron millet germplasm, using remote edge sexual hybridization, the method breeding being then returned again, packet
Include following steps:
(1), using anti-nicosulfuron Green foxtail material as male parent, distant hybridization is carried out to cultivate millet to be maternal, F1 plants
Strain Seedling Stage sprays nicosulfuron, obtains the hybrid with nicosulfuron resistance；
(2), to cultivate millet as recurrent parent, by hybrid and millet progress 2-4 generation backcrossing is cultivated, selects resistance single plant certainly
2-3 generation is handed over, filtering out Agronomic is millet and the seed with nicosulfuron resistance, obtains anti-nicosulfuron millet
New germ plasm.
Anti- nicosulfuron herba setariae viridis material is obtained by following methods:
A, from the Green foxtail material of anti-imazethapyr, screening has both the Green foxtail material of anti-nicosulfuron character
Material；
B, the anti-imazethapyr Green foxtail material of anti-nicosulfuron character is subjected to the screening of nicosulfuron resistance power,
The material for selecting nicosulfuron resistance, obtains anti-nicosulfuron Green foxtail material.
Male parent, female parent are hybridized by the way of sowing respectively, include the following steps: maternal normal plantation, line-spacing
0.6-0.8cm, male parent are planted in flowerpot, encounter the male parent with maternal flower synchronization, and transplanting male parent to planting of maternal area carries out
Hybridization.The present invention control line-spacing 0.6-0.8cm just in be spaced a line, convenient for centre let off fireworks basin hybridization, line-spacing be greater than 0.8cm,
The flowerpot put cannot simultaneously and both sides hybridization of female parent, reduction efficiency；Line-spacing is less than 0.6 ㎝, and spacing is a bit small, it has not been convenient to flowerpot
It is mobile, it is also more difficult to do operation when hybridizing.
The nicosulfuron that sprays is 95.5% nicosulfuron raw medicine, and sprayed dose range is 30-60g a.i./hm2。
Monoclonal antibody nicosulfuron strain kind is cultivated using anti-nicosulfuron millet new germ plasm；It is new using anti-nicosulfuron millet
Germplasm cultivates the multiline variety of " the homotype Sister Lines or near isogenic lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition.
The cultivation of monoclonal antibody nicosulfuron strain kind: using the millet new germ plasm of anti-nicosulfuron as male parent, with not anti-weeding
The backbone millet parent material of agent hybridizes, and F1 generation sprays the pseudostationary that nicosulfuron kills not antiweed, is then selfed 4-5
In generation, goes out monoclonal antibody nicosulfuron Millet Variety in advanced lines final election.
The multiline variety of " the homotype Sister Lines or near isogenic lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition
It cultivates: using the millet new germ plasm of anti-nicosulfuron as male parent, hybridizing with the backbone millet parent material of not antiweed, F1 generation spray
The pseudostationary that nicosulfuron kills not antiweed is applied, is selfed 4-5 generation or then with the backbone millet parent of not antiweed
Material is recurrent parent, is returned 4-5 generation, goes out anti-nicosulfuron millet new lines, while breeding and the anti-cigarette in advanced lines final election
The Sister Lines or near isogenic lines of the identical not anti-nicosulfuron of Sulfometuron Methyl millet new lines Comprehensive Traits, the two mixture, obtain
The multiline variety of " the homotype Sister Lines or near isogenic lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition.
In the cultivation of the multiline variety of " the homotype Sister Lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition, F1 generation
It sprays nicosulfuron to kill not after the pseudostationary of antiweed, proceed as follows:
A, F2, F3 spray nicosulfuron for Seedling Stage and kill the not paddy seedling of antiweed completely, select agronomy before mature
The excellent single plant of shape, and the identification of seedling stage antiweed is carried out to the Some seeds for each single plant harvest that F2, F3 generation are selected, it eliminates
The single plant of completely anti-or inwhole antiweeds, leaves resistance and there is isolated single plant；
B, later each seedling stage generation in F4 generation no longer sprays nicosulfuron, carries out in the excellent family of economical character before mature
Single-plant selection, i.e. full seed, the single plant that pest and disease damage is small, lodging is light, and seedling stage is carried out to the Some seeds of each single plant harvest
Antiweed identification, the anti-single plant with not anti-nicosulfuron of selection are next-generation by the anti-strain except nicosulfuron, not anti-nicosulfuron
System compares selection, until selecting, each character is stable, the identical anti-nicosulfuron millet new lines of Comprehensive Traits, not anti-cigarette
The Sister Lines of Sulfometuron Methyl.
In the cultivation of the multiline variety of " near isogenic lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition, F1 generation
It sprays nicosulfuron to kill not after the pseudostationary of antiweed, proceed as follows:
A, using the backbone millet parent material of not antiweed as recurrent parent, it is returned 4-5 generation, per generation backcrossing is in seedling stage
Spray nicosulfuron；
B, economical character elite seedling is selected when last time backcross population maturation, i.e., full seed, pest and disease damage are small, lodge
Light single plant, then it is selfed 2-3 generation, the millet new lines of anti-nicosulfuron are therefrom selected, while it is identical to select Comprehensive Traits
The near isogenic lines of not anti-nicosulfuron, the two mixture form multiline variety.
The beneficial effects of the present invention are: the present invention utilizes the method for sexual hybridization and backcrossing by the phonetic sulphur of anti-cigarette of Green foxtail
Grand gene transfer formulates out the Millet Breeding new germ plasm of anti-nicosulfuron into cultivation millet, for the paddy for cultivating anti-nicosulfuron
Sub- new varieties establish material foundation.The anti-nicosulfuron millet new germ plasm economical character that method of the invention selects is preferable, has
There is nicosulfuron resistance.Selection hybridization procedures of the invention simplify, and improve hybridization, are returned successfully accuracy, confidence level.
Male parent, female parent are hybridized by the way of sowing respectively, and hybridization success rate is high, and it is less to solve male parent grain weight
And the problem of male parent, maternal flowering asynchronism.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below.
Embodiment 1
1, the acquisition of anti-nicosulfuron Green foxtail material:
2006, Grain Institute, Provincial Academy of Agriculture and Forestry, Hebei Provinee was from your Er Fu university (University of of Canada
Guelph, Canada) introduce the Green foxtail material (Setaria viridis) of anti-imazethapyr (Imazethapyr).
The material for having both anti-nicosulfuron character is filtered out in Green foxtail material from anti-imazethapyr in 2011；It will
The anti-imazethapyr Green foxtail material of anti-nicosulfuron character carries out the screening of nicosulfuron resistance power, selects the phonetic sulphur of anti-cigarette
The strong material of grand character obtains anti-nicosulfuron Green foxtail material, is named as anti-nicosulfuron herba setariae viridis 1S herein.
2, preparing hybrid combines:
Winter in 2011 and spring in 2012, in greenhouse with paddy 12 of weighing for female parent, with the Green foxtail 1S of anti-nicosulfuron
15 are combined for male parent preparing hybrid.
3, the acquisition of anti-nicosulfuron millet germplasm:
Summer in 2012 filters out the anti-nicosulfuron material of F1 generation 10.Simultaneously with cultivation millet variety (Ji Gu 31, Ji
Paddy 32, Jin Gu 1, Henan paddy 18) hybridization (, winter in 2012 obtains F1 generation, and 3 leaf phases sprayed nicosulfuron and leave the miscellaneous of antiweed
Kind, continue to be returned with backbone millet variety；The 3 leaf phases of generation spring F2 in 2013 spray nicosulfuron, leave the hybrid of antiweed,
Elite seedling is selected to continue to be returned with backbone millet variety, the 3 leaf phases of generation summer F3 in 2013 spray nicosulfuron, carry out before mature
A large amount of Single-plant selection, and the identification of seedling stage antiweed is carried out to the Some seeds of each single plant harvest, select anti-nicosulfuron
Single plant, through winter in 2013, two generation of spring in 2014, summer in 2014 filter out economical character preferably, have nicosulfuron
The millet new germ plasm 14H06 of resistance.Germplasm plant height 115.6cm, breeding time 86 days, economical character was preferable, had nicosulfuron
Resistance has established material foundation to cultivate the Millet Variety of anti-nicosulfuron.
14H06 is from combination " Henan paddy 18 × (weighing apparatus paddy 12 × anti-nicosulfuron herba setariae viridis 1S) ".Wherein, Henan paddy 18 is river
The Millet Variety of southern Anyang academy of agricultural sciences breeding, weighing apparatus paddy 12 is precocious millet variety, and anti-nicosulfuron herba setariae viridis 1S is from drawing
Into anti-imazethapyr herba setariae viridis material in the material of better resistance that filters out.
Embodiment 2, hybridization technique
Anti- nicosulfuron herba setariae viridis material 1S breeding time is too short, and plant is short and small, it is not easy to hybridize with millet.Traditional method
It is interval sowing.But for anti-nicosulfuron material, breeding time is too short, the florescence is not easy to encounter, and grain weight is limited, no
Convenient for designing excessive gradient；And plant is short and small, and it is more difficult in operation when hybridizing with millet.By groping repeatedly, we are adopted
It takes using anti-nicosulfuron herba setariae viridis material as male parent, take backbone millet variety as maternal, male parent, the maternal method sowed respectively:
Different millet varieties make female parent, and normally in field planting, male parent divides different times precision drilling in flowerpot, encounters flower synchronization
Female parent, male parent flowerpot is moved near female parent, suitable distance is reserved according to the maternal plant height with male parent, in order to female parent
Bending is hybridized with male parent.The method hybridization success rate is higher, has stronger operability.
The application of embodiment 3, anti-nicosulfuron Millet Variety
Anti- nicosulfuron millet new germ plasm can be applied to the following aspects:
(1) monoclonal antibody nicosulfuron kind is cultivated using anti-nicosulfuron millet germplasm: with the millet novel species of anti-nicosulfuron
Matter is male parent, hybridize with the backbone millet parent material of not antiweed, and F1 generation sprays nicosulfuron and kills not antiweed
Then pseudostationary is selfed 4-5 generation, goes out monoclonal antibody nicosulfuron Millet Variety in advanced lines final election.Wherein, between precision drilling not
Seedling is suitable for application rate (kg/hm2Suitable (ten thousand plants/hm of the density of reserved seedlings of)=local2) × kind mass of 1000 kernel ÷ germination percentage ÷ emergence rate
÷ survival rate of seedlings ÷ 100,2-5 the leaf phase spray nicosulfuron, can kill weeds；
(2) monoclonal antibody nicosulfuron strain kind is cultivated using anti-nicosulfuron millet new germ plasm；Utilize anti-nicosulfuron paddy
Sub- new germ plasm cultivates the polyphyly of " the homotype Sister Lines (or near isogenic lines) of the antiweed of monoclonal antibody nicosulfuron strain+not " composition
Kind.
The cultivation of monoclonal antibody nicosulfuron strain kind: using the millet new germ plasm of anti-nicosulfuron as male parent, with not anti-weeding
The backbone millet parent material of agent hybridizes, and F1 generation sprays the pseudostationary that nicosulfuron kills not antiweed, is then selfed 4-5
In generation, goes out monoclonal antibody nicosulfuron Millet Variety in advanced lines final election, may be directly applied to produce, and uses nicosulfuron
Learn weeding.
The multiline variety of " the homotype Sister Lines (or near isogenic lines) of the antiweed of monoclonal antibody nicosulfuron strain+not " composition
Cultivation: using the millet new germ plasm of anti-nicosulfuron as male parent, hybridize with the backbone millet parent material of not antiweed, F1 generation
The pseudostationary that nicosulfuron kills not antiweed is sprayed, is then selfed 4-5 generation (or with the backbone millet parent of not antiweed
This material is recurrent parent, is returned 4-5 generation), go out to meet the anti-nicosulfuron millet new lines of objective trait in advanced lines final election,
The Sister Lines (or near isogenic lines) of breeding and the almost the same not anti-nicosulfuron of the kind Comprehensive Traits simultaneously, the two press one
Certainty ratio mixture (such as 1: 1), obtain " the homotype Sister Lines of the antiweed of monoclonal antibody nicosulfuron strain+not (or near isogene
System) " composition multiline variety.
The multiline variety of " anti-nicosulfuron strain+not antiweed homotype Sister Lines " composition is obtained using following methods
:
A, F2, F3 spray nicosulfuron for Seedling Stage and kill the not paddy seedling of antiweed completely, it is mature before the excellent list of selection
Strain, and the identification of seedling stage antiweed is carried out to the Some seeds for each single plant harvest that F2, F3 generation are selected, eliminate it is completely not anti-or
The single plant of whole antiweeds leaves resistance and there is isolated single plant；
B, F4 generation later no longer herbicide spraying of each seedling stage generation, carries out a large amount of single plant before mature in excellent family
Selection, and the identification of seedling stage antiweed is carried out to the Some seeds of each single plant harvest, selection resists and the list of not anti-nicosulfuron
Strain, the next generation is not by antiweed, the strain of antiweed compares selection, until selecting each character stabilization, comprehensive
The Sister Lines of the almost the same antiweed of shape, not antiweed.
The multiline variety of " anti-nicosulfuron strain+not antiweed near isogenic lines " composition is obtained using following methods
:
A, using the millet new germ plasm of anti-nicosulfuron as male parent, hybridize with the backbone millet parent material of not antiweed,
F1 generation sprays the pseudostationary that nicosulfuron kills not antiweed, using the backbone millet parent material of not antiweed as circulation parent
This, is returned 4-5 generation, and per generation backcrossing sprays nicosulfuron in seedling stage.
B, fine individual plant is largely selected when last time backcross population maturation, then was selfed for 2 generations, therefrom selects anti-nicosulfuron
Millet new lines, while selecting the near isogenic lines of the almost the same not anti-nicosulfuron of Comprehensive Traits, the two is by certain
Ratio mixture forms multiline variety.
Wherein, semi-accuracy seeding, the two application rate ratio 1:(1-1.5), wherein anti-nicosulfuron strain application rate (kg/
hm2Suitable (ten thousand plants/hm of the density of reserved seedlings of)=local2) × kind mass of 1000 kernel ÷ germination percentage ÷ emergence rate ÷ survival rate of seedlings ÷ 100,2-5
The leaf phase sprays the homotype Sister Lines or near isogenic lines that nicosulfuron kills not antiweed, realizes chemical thinning, and kill miscellaneous
Grass.
Embodiment 4
The resistant analysis of antiweed nicosulfuron:
(1) the anti-nicosulfuron millet germplasm genetics of resistance of the present invention is stablized, and inbreeding of more generation resistance level does not change.
(2) anti-sense material hybridization, first-filial generation show antiweed, and F2 generation meets the anti-sense separation of 3:1, first backcross generation symbol
Close the anti-sense separation of 1:1.Just giving reciprocal cross result consistent.Illustrate the anti-nicosulfuron character of millet by a pair of of core dominant gene control
System.
Embodiment 5
Second effect analysis of anti-nicosulfuron millet germplasm resistant gene:
(1) at different densities (30 plants/square metre, 50 plants/square metre), three kinds of different modes plant anti-sense near isogenic lines
(100% anti-nicosulfuron group, 50% resistance, 50% perceptual population mixture, 100% perceptual group), 5 repetitions, per small
15 square metres of area；
(2) different times investigate economical character, by comparing different groups, the anti-sense near isogenic lines of different condition
Result；
(3) result of study shows that millet obtains antiweed nicosulfuron gene and will not reduce economical character level effect.
Selection of the invention is by distant hybridization method by the base of the antiweed nicosulfuron in Green foxtail
Because importeding into cultivar millet, resistance is then obtained by backcrossing and reaches good anti-of realistic scale, inheritance stability, economical character
Nicosulfuron herbicide agent millet new germ plasm, belongs to non-transgenic means, further enriches to millet and imports external source desirable genes
Technology path.
The present invention formulates anti-nicosulfuron herbicide agent millet new germ plasm using distant hybridization method, is not belonging to transgenosis model
Farmland further enriches the technology path that external source desirable genes are imported to millet；Meanwhile it proposing and cultivating anti-sense nicosulfuron elder sister
Younger sister is to form multiline variety, and the thinning of millet chemistry is realized while chemical weed control, realizes the efficient benefit of anti-herbicide gene
With.
The present invention using Parent hybridization by the way of, have the advantage that,
(1) maternal normal plantation, line-spacing 0.6-0.8cm, male parent are planted in flowerpot, encounter the father with maternal flower synchronization
This, transplanting male parent to planting of maternal area is hybridized.
Advantage:
A, male parent is anti-nicosulfuron herba setariae viridis material, and breeding time is short, it is not easy to maternal flower synchronization, by its kind in flower
It in basin, and in the ground by maternal a large amount of plantation, is flexibly moved convenient for male parent, encounters florescence suitable female parent and move to appropriate area and do
Hybridization；
B, paternal plant is short and small, can increase its height using flowerpot, if still not enough, thing can be padded under flowerpot；
C, male parent grain weight is small, is conducive to save grain weight in this way.
(2) in the ground by male parent interlacing kind, other region by stages, largely plantation it is maternal, encounter the father of flower synchronization
Female parent, transplanting are maternal to male parent interlacing region.
Advantage:
A, male parent is in field planting, and growing way is good, high survival rate.
B, alternative maternal range is big, and male parent utilization rate is high.
Claims (6)
1. the selection of anti-nicosulfuron millet germplasm, using remote edge sexual hybridization, the method breeding being then hybridized is special
Sign is, comprising the following steps:
(1), using anti-nicosulfuron Green foxtail material as male parent, distant hybridization, F1 plant children are carried out to cultivate millet to be maternal
Seedling spraying nicosulfuron obtains the hybrid with nicosulfuron resistance；
(2), hybrid is subjected to 2-4 for hybridizing with cultivation millet, selects resistance individual plant selfing 2-3 generation, filters out economical character table
Now for millet and with the seed of nicosulfuron resistance, anti-nicosulfuron millet new germ plasm is obtained；
Anti- nicosulfuron herba setariae viridis material is obtained by following methods:
A, from the Green foxtail material of anti-imazethapyr, screening has both the Green foxtail material of anti-nicosulfuron character；
B, the anti-imazethapyr Green foxtail material of anti-nicosulfuron character is subjected to the screening of nicosulfuron resistance power, selection
The material of nicosulfuron resistance obtains anti-nicosulfuron Green foxtail material；
Male parent, female parent are hybridized by the way of sowing respectively, include the following steps: maternal normal plantation, line-spacing 0.6-
0.8cm, male parent are planted in flowerpot, encounter the male parent with maternal flower synchronization, and transplanting male parent to planting of maternal area is hybridized.
2. a kind of application for the anti-nicosulfuron millet germplasm that selection according to claim 1 obtains, which is characterized in that
Monoclonal antibody nicosulfuron strain kind is cultivated using anti-nicosulfuron millet new germ plasm；Utilize anti-nicosulfuron millet new germ plasm
Cultivate the multiline variety of " the homotype Sister Lines or near isogenic lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition.
3. the anti-nicosulfuron millet germplasm that a kind of selection according to claim 1 according to claim 2 obtains
Application, which is characterized in that the cultivation of monoclonal antibody nicosulfuron strain kind: using the millet new germ plasm of anti-nicosulfuron as male parent,
Hybridize with the backbone millet parent material of not antiweed, F1 generation sprays the pseudostationary that nicosulfuron kills not antiweed, so
It is selfed 4-5 generation afterwards, goes out monoclonal antibody nicosulfuron Millet Variety in advanced lines final election.
4. the anti-nicosulfuron millet germplasm that a kind of selection according to claim 1 according to claim 2 obtains
Application, which is characterized in that " the homotype Sister Lines or near isogenic lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition
The cultivation of multiline variety: the backbone millet parent material using the millet new germ plasm of anti-nicosulfuron as male parent, with not antiweed
Hybridization, F1 generation spray the pseudostationary that nicosulfuron kills not antiweed, are selfed 4-5 generation or then with the bone of not antiweed
The sub- parent material of gulch is recurrent parent, is returned 4-5 generation, goes out anti-nicosulfuron millet new lines in advanced lines final election, select simultaneously
Educate the Sister Lines or near isogenic lines of not anti-nicosulfuron identical with the anti-nicosulfuron millet new lines Comprehensive Traits, the two
Mixture obtains the multiline variety of " the homotype Sister Lines or near isogenic lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition.
5. the anti-nicosulfuron millet germplasm that a kind of selection according to claim 1 according to claim 4 obtains
Application, which is characterized in that the training of the multiline variety of " the homotype Sister Lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition
In educating, F1 generation sprays nicosulfuron and kills not after the pseudostationary of antiweed, proceeds as follows:
A, F2, F3 spray nicosulfuron for Seedling Stage and kill the not paddy seedling of antiweed completely, it is mature before selection full seed
Single plant, and the identification of seedling stage antiweed is carried out to the Some seeds for each single plant harvest that F2, F3 generation are selected, it eliminates and does not resist completely
Or the single plant of whole antiweeds, it leaves resistance and there is isolated single plant；
B, later each seedling stage generation in F4 generation no longer sprays nicosulfuron, Single-plant selection is carried out before mature, and harvest to each single plant
Some seeds carry out the identification of seedling stage antiweed, selection is anti-and the single plant of not anti-nicosulfuron, it is next-generation by anti-nicosulfuron,
The strain of not anti-nicosulfuron compares selection, until selecting, each character is stable, the identical anti-nicosulfuron of Comprehensive Traits
The Sister Lines of millet new lines, not anti-nicosulfuron.
6. the anti-nicosulfuron millet germplasm that a kind of selection according to claim 1 according to claim 4 obtains
Application, which is characterized in that the training of the multiline variety of " near isogenic lines of the antiweed of monoclonal antibody nicosulfuron strain+not " composition
In educating, F1 generation sprays nicosulfuron and kills not after the pseudostationary of antiweed, proceeds as follows:
A, using the backbone millet parent material of not antiweed as recurrent parent, it is returned 4-5 generation, per generation backcrossing sprays in seedling stage
Nicosulfuron；
B, the single plant of full seed is selected when last time backcross population maturation, then is selfed 2-3 generation, therefrom selects anti-nicosulfuron
Millet new lines, while selecting the near isogenic lines of the identical not anti-nicosulfuron of Comprehensive Traits, the two mixture forms more
It is kind.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610817719.3A CN106376454B (en) | 2016-09-13 | 2016-09-13 | The selection of anti-nicosulfuron millet germplasm and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610817719.3A CN106376454B (en) | 2016-09-13 | 2016-09-13 | The selection of anti-nicosulfuron millet germplasm and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106376454A CN106376454A (en) | 2017-02-08 |
| CN106376454B true CN106376454B (en) | 2018-12-25 |
Family
ID=57936380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610817719.3A Active CN106376454B (en) | 2016-09-13 | 2016-09-13 | The selection of anti-nicosulfuron millet germplasm and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106376454B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106857128A (en) * | 2017-02-16 | 2017-06-20 | 贵州红四方农业发展股份有限公司 | A kind of hybrid rice male parent rice transplanting method |
| CN108575735A (en) * | 2018-07-19 | 2018-09-28 | 安阳市农业科学院 | A kind of breeding method of rapidly and efficiently selection and breeding eurytopicity millet variety |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586159A (en) * | 2004-08-13 | 2005-03-02 | 河北省农林科学院谷子研究所 | Simplifying cultivating millet variety breeding and its cultivating method |
| CN105706917A (en) * | 2016-03-30 | 2016-06-29 | 河北省农林科学院谷子研究所 | Breeding method of special foxtail millet for forage grass |
| CN105861734A (en) * | 2016-06-17 | 2016-08-17 | 河北省农林科学院谷子研究所 | Rapid identification method for homozygosis, heterozygosis and resistance-free performance of anti-nicosulfuron genes of foxtail millet |
-
2016
- 2016-09-13 CN CN201610817719.3A patent/CN106376454B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586159A (en) * | 2004-08-13 | 2005-03-02 | 河北省农林科学院谷子研究所 | Simplifying cultivating millet variety breeding and its cultivating method |
| CN105706917A (en) * | 2016-03-30 | 2016-06-29 | 河北省农林科学院谷子研究所 | Breeding method of special foxtail millet for forage grass |
| CN105861734A (en) * | 2016-06-17 | 2016-08-17 | 河北省农林科学院谷子研究所 | Rapid identification method for homozygosis, heterozygosis and resistance-free performance of anti-nicosulfuron genes of foxtail millet |
Non-Patent Citations (3)
| Title |
|---|
| Green foxtail (Setaria viridis) resistance to acetolactate synthase inhibitors;D.S. Volenberg et al.;《Phytoprotection》;20021231;第83卷(第2期);第99-109页 * |
| 应用种间杂交方法进行谷子抗除草剂育种的初步研究;马金丰等;《黑龙江农业科学》;20051231(第2期);第18-20页 * |
| 抗除草剂谷子新种质的创制、鉴定与利用;王天宇等;《中国农业科技导报》;20001231(第5期);第1-5页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106376454A (en) | 2017-02-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Barrett | Crop mimicry in weeds | |
| Suman | Plant tissue culture: A promising tool of quality material production with special reference to micropropagation of banana. | |
| CN105010129B (en) | Precocious upland cotton new germ plasm selection | |
| CN108925423B (en) | A kind of heat-resisting germplasm selection of Rise's boot period | |
| CN106665332A (en) | Method for carrying out recurrent selection breeding on water-saving and drought-resisting rice by utilizing dominant nuclear male sterile material | |
| Ninou et al. | Wheat landraces are better qualified as potential gene pools at ultraspaced rather than densely grown conditions | |
| CN108967182A (en) | A kind of selection of precocious, high ginning outturn cotton line | |
| Yakushiji et al. | Ceratocystis canker resistance in BC1 populations of interspecific hybridization of fig (Ficus carica) and F. erecta | |
| CN103975810B (en) | A kind of paddy rice companion planting method | |
| CN102090326A (en) | Method for cultivating wheat cultivar | |
| CN105961192B (en) | A kind of method using the Backcrossing methods transformation Chinese sorghum sterile line when stablizing | |
| CN106376454B (en) | The selection of anti-nicosulfuron millet germplasm and application | |
| CN106613900A (en) | Breeding and cultivating method for millet varieties resisting sethoxydim and imidazolone herbicides | |
| Majidian et al. | The effect of four levels of GA3 and BA on the quantitative and qualitative characteristics of Zantedeschia aethiopica cv. Childsiana pot plant | |
| CN108935079B (en) | Simplified seed production method of rape hybrid F1 seed | |
| CN106718850A (en) | A kind of breeding method of muskmelon cenospecies | |
| CN106508671A (en) | Method for breeding earlier germinated eriobotrya japonica seeds | |
| CN108419670A (en) | A kind of long fringe neck novel rice sterile line breeding method | |
| Hladni et al. | Sunflower and abiotic stress: genetics and breeding for resistance in the—omics era sunflower abiotic stress breeding | |
| Mahmud et al. | Development of mutants in sugarcane through callus culture | |
| CN101171906A (en) | Tobacco gamma-ray and mutagenesis idioplasm innovation and breed selecting and cultivating technique | |
| Rakha | Interspecific hybridization between Cucurbita Maxima Duch. and Cucurbita moschata Duch. for high efficient development of Cucurbit Rootstocks | |
| CN103238515B (en) | New rice variety breeding method | |
| CN109042309A (en) | A kind of breeding method of high yielding soybeans | |
| Mogili et al. | Mulberry breeding for higher leaf productivity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |