CN109042309A - A kind of breeding method of high yielding soybeans - Google Patents
A kind of breeding method of high yielding soybeans Download PDFInfo
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- CN109042309A CN109042309A CN201811139624.6A CN201811139624A CN109042309A CN 109042309 A CN109042309 A CN 109042309A CN 201811139624 A CN201811139624 A CN 201811139624A CN 109042309 A CN109042309 A CN 109042309A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to crop breeding technical field, in particular to a kind of breeding method of high yielding soybeans.Choosing the soil that pH is 5.8-7.3 is planting site, and isolated area is arranged in planting site surrounding；Choosing high yielding soybeans strain is female parent, and choosing the stable wild soybean strain of character is male parent, and hybridization obtains F1 generation；It is selfed a generation after F1 generation plantation, obtains F2 generation；Using F2 generation as male parent, continue to be returned with former female parent, until screening yield and female parent line yield comparable Fm generation；Fm generation continuous selfing, until obtaining character stable high yield Fn generation.The present invention combines a variety of breeding modes such as hybridization, backcrossing, selfing and mutation that can efficiently obtain the new soybean varieties of high yield, enriches Soybean genetic resources.
Description
Technical field
The invention belongs to crop breeding technical field, in particular to a kind of breeding method of high yielding soybeans.
Background technique
Soybean scientific name is max (L) Merrill, alias: soya bean (including green soya bean, semen sojae atricolor, purple beans and spot tea Giycine beans
Deng).Soybean belongs to pulse family, Papillionoideae, Glycine, Soybean Species.It is annual herb plant, originates in China.China is from ancient times
Cultivation has plantation history in 5000 so far.The whole nation is generally planted now, in northeast, North China, Shan, river and Lower Changjiang River region
Produce, it is more with the Yangtze river basin and southwest cultivation, with northeast soybean optimal quality.
Nearly 40 years, the soybean area in the world expanded 3 times, and total output increases 6 times, and the China of its cradle, development
But very slowly, lower mainly due to soybean price at this stage, and soybean acreage is caused to reduce.Soybean is extricated oneself from a plight most
Good method is to improve per unit area yield and reduce production cost, needs scientific research personnel that more high yields, high quality soybean new varieties are pushed to city
?.Soybean is self pollination crop, and selfing rate is less than 1%, and intensive agriculture production keeps soybean varieties increasingly single, hereditary basis
Increasingly narrow, resistance is gradually reduced.Therefore, the breakthrough and progress of soybean breeder from now on, should be dependent on fine germplasm resources
It widens, improve and applies.At present there is breakthrough transgenic breeding method to cause heated argument in the world, recognizes
There is great potential danger for genetically modified crops, may threaten to human health and environment for human survival.Therefore, such as
What, which finds one kind, can break through traditional biological breeding low-level repetition operation and change the breeding of gene character, safety and environmental protection
Means become the task of top priority.
For this purpose, the application has studied a kind of breeding method of high yielding soybeans.
Summary of the invention
A variety of breeding modes such as present invention combination hybridization, backcrossing, selfing and mutation can efficiently obtain the soybean new product of high yield
Kind, enrich Soybean genetic resources.
The present invention provides a kind of breeding methods of high yielding soybeans, comprising the following steps:
S1, choosing the soil that pH is 5.8-7.3 is planting site, and isolated area is arranged in planting site surrounding；
S2, in spring, it is female parent that high yielding soybeans strain is chosen at 10-15 DEG C of temperature on average, chooses stable wild big of character
Beans strain is male parent, and both makes flower synchronization, maternal emasculation and and paternal hybrid, obtain F1 generation；
S3, is selfed a generation after F1 generation is planted, and F1 generation germination, to Seedling Stage 40W ultraviolet light, frequency is
2-3d/ times, each 20-30min obtains F2 generation；
In S4, the F2 using in S3 generation, are returned as the female parent line selected in male parent, with S2, and production is chosen from backcross progeny
High strain is measured as male parent, lasting backcrossing, until screening yield and female parent line yield comparable Fm generation；
In Fm generation in S4, is selfed by S5, and the strain that then per generation filters out high yield stalwartness enters next round selfing, until
Obtain character stable high yield Fn generation.
Preferably, to soil deep ploughing 25-35cm before the planting site plantation in S1,200-300kg/ mus apply muck and filling
It irrigates, makes soil moisture content up to 70%-80%.
Preferably, female parent line is one of the Changjiang river spring No. 1, Chongqing beans 2, Chongqing beans 9, Shanxi beans 23 or several in S2
Kind.
Preferably, female parent uses bunch planting when hybridizing or be returned, and every cave 2-3 seeds, spacing in the rows 50-80cm, male parent is using single
Grain program request, row plantation between male parent and female parent.
Compared with prior art, beneficial effects of the present invention:
The present invention is hybridized using merits such as saline-alkali tolerant, the disease-resistant, cold-resistants of wild species soybean with existing high-yield variety, is led to
It crosses a selfing generation and mutagenesis obtains slightly stable wild characters, high-yielding strain is then obtained by backcrossing, recycle selfing to reinforce high
It produces characteristic, stablize other characters, the present invention combines a variety of breeding modes such as hybridization, backcrossing, selfing and mutation that can efficiently obtain height
The new soybean varieties of production, enriches Soybean genetic resources.
Specific embodiment
Several specific embodiments of the invention are described in detail below, it is to be understood that protection scope of the present invention
It is not limited by the specific implementation.Experimental method used in embodiment is conventional method unless otherwise specified, real
Material used in example is applied, is commercially available.
Wild soybean strain: for the distinctive wild soybean kind in the Huanghe delta, Agricultural University Of Nanjing's soybean resource is derived from
Library；The Changjiang river spring No. 1: combining training with nuclear technology research on utilization institute and Chongqing Agricultural Academy of Science by Zhejiang Academy of Agricultural Science crop
The new soybean varieties educated；Chongqing beans 2 and beans 9, Chongqing: Chongqing Academy of Agricultural Sciences is derived from；Shanxi beans 23: academy of agricultural sciences of Shanxi Province warp
Ji crop research institute.
Embodiment 1
A kind of breeding method of high yielding soybeans, comprising the following steps:
S1, choosing the soil that pH is 5.8 is planting site, and soil deep ploughing 25cm, 200kg/ mus apply muck and irrigation, make soil
Isolated area is arranged up to 70%, and in planting site surrounding in earth water content；
S2, in spring, it is female parent that high yielding soybeans strain is chosen at 10 DEG C of temperature on average, and female parent line is the Changjiang river spring No. 1, Chongqing beans
No. 2, choosing character to stablize excellent (saline-alkali tolerant, disease-resistant, cold-resistant) wild soybean strain is male parent, and makes the two flower synchronization,
Maternal emasculation and and paternal hybrid, obtain F1 generation；
S3, is selfed a generation after F1 generation is planted, and F1 generation germination, to Seedling Stage 40W ultraviolet light, frequency is
2-3d/ times, each 20min obtains F2 generation；
In S4, the F2 using in S3 generation, are returned as the female parent line selected in male parent, with S2, and production is chosen from backcross progeny
High strain is measured as male parent, lasting backcrossing, until screening yield and female parent line yield comparable F6 generation；
Female parent uses bunch planting when hybridization or backcrossing, and every cave 2-3 seeds, spacing in the rows 50cm, male parent is using simple grain program request, male parent
The row plantation between female parent；
In F6 generation in S4, is selfed by S5, and the strain that then per generation filters out high yield stalwartness enters next round selfing, until
Obtain character stable high yield F9 generation.The F9 generation that embodiment 1 obtains yield under identical planting conditions is higher than the maternal the Changjiang river spring 1
Number, Chongqing beans 2, illustrate breeding method of the invention have application prospect.
Embodiment 2
A kind of breeding method of high yielding soybeans, comprising the following steps:
S1, choosing the soil that pH is 7.3 is planting site, and soil deep ploughing 35cm, 300kg/ mus apply muck and irrigation, make soil
Isolated area is arranged up to 80%, and in planting site surrounding in earth water content；
S2, in spring, it is female parent that high yielding soybeans strain is chosen at 15 DEG C of temperature on average, and female parent line is beans 9, Chongqing, the property chosen
It is male parent that shape, which stablizes excellent (saline-alkali tolerant, disease-resistant, cold-resistant) wild soybean strain, and makes the two flower synchronization, and maternal emasculation is simultaneously
With paternal hybrid, F1 generation is obtained；
S3, is selfed a generation after F1 generation is planted, and F1 generation germination, to Seedling Stage 40W ultraviolet light, frequency is
3d/ times, each 30min obtains F2 generation；
In S4, the F2 using in S3 generation, are returned as the female parent line selected in male parent, with S2, and production is chosen from backcross progeny
High strain is measured as male parent, lasting backcrossing, until screening yield and female parent line yield comparable F5 generation；
Female parent uses bunch planting when hybridization or backcrossing, and every cave 2-3 seeds, spacing in the rows 80cm, male parent is using simple grain program request, male parent
The row plantation between female parent；
In F5 generation in S4, is selfed by S5, and the strain that then per generation filters out high yield stalwartness enters next round selfing, until
Obtain character stable high yield F10 generation.The F10 generation that embodiment 2 obtains yield under identical planting conditions is higher than maternal Chongqing beans 9
Number, illustrate that breeding method of the invention has application prospect.
Embodiment 3
A kind of breeding method of high yielding soybeans, comprising the following steps:
S1, choosing the soil that pH is 6.3 is planting site, and soil deep ploughing 29cm, 250kg/ mus apply muck and irrigation, make soil
Isolated area is arranged up to 75%, and in planting site surrounding in earth water content；
S2, in spring, it is female parent that high yielding soybeans strain is chosen at 13 DEG C of temperature on average, and female parent line is one in Shanxi beans 23
Kind is several, and choosing character to stablize excellent (saline-alkali tolerant, disease-resistant, cold-resistant) wild soybean strain is male parent, and makes the two florescence
Meet, maternal emasculation and and paternal hybrid, obtain F1 generation；
S3, is selfed a generation after F1 generation is planted, and F1 generation germination, to Seedling Stage 40W ultraviolet light, frequency is
2-3d/ times, each 25min obtains F2 generation；
In S4, the F2 using in S3 generation, are returned as the female parent line selected in male parent, with S2, and production is chosen from backcross progeny
High strain is measured as male parent, lasting backcrossing, until screening yield and female parent line yield comparable F6 generation；
Female parent uses bunch planting when hybridization or backcrossing, and every cave 2-3 seeds, spacing in the rows 70cm, male parent is using simple grain program request, male parent
The row plantation between female parent；
In F6 generation in S4, is selfed by S5, and the strain that then per generation filters out high yield stalwartness enters next round selfing, until
Obtain character stable high yield F11 generation.The F11 generation that embodiment 3 obtains yield under identical planting conditions is higher than maternal Shanxi beans 23
Number, illustrate that breeding method of the invention has application prospect.
It should be noted that the step method used in claims of the present invention is same as the previously described embodiments, in order to anti-
It only repeats, description of the invention preferred embodiment, once a person skilled in the art knows basic creative general
It reads, then additional changes and modifications can be made to these embodiments.So it includes preferred real that the following claims are intended to be interpreted as
It applies example and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (4)
1. a kind of breeding method of high yielding soybeans, which comprises the following steps:
S1, choosing the soil that pH is 5.8-7.3 is planting site, and isolated area is arranged in planting site surrounding；
S2, in spring, it is female parent that high yielding soybeans strain is chosen at 10-15 DEG C of temperature on average, chooses the stable wild soybean product of character
System is male parent, and both makes flower synchronization, maternal emasculation and and paternal hybrid, obtain F1 generation；
S3, is selfed a generation after F1 generation is planted, and F1 generation germination is to Seedling Stage 40W ultraviolet light, frequency 2-3d/
Secondary, each 20-30min obtains F2 generation；
In S4, the F2 using in S3 generation, are returned as the female parent line selected in male parent, with S2, and yield height is chosen from backcross progeny
Strain as male parent, it is lasting to be returned, until screening yield and female parent line yield comparable Fm generation；
In Fm generation in S4, is selfed by S5, and the strain that then per generation filters out high yield stalwartness enters next round selfing, until obtaining
Character stable high yield Fn generation.
2. the breeding method of high yielding soybeans as described in claim 1, which is characterized in that soil before the planting site plantation in S1
Deep plough 25-35cm, and 200-300kg/ mus apply muck and irrigation, makes soil moisture content up to 70%-80%.
3. the breeding method of high yielding soybeans as described in claim 1, which is characterized in that in S2 female parent line be the Changjiang river spring No. 1,
Change one or more of beans 2, Chongqing beans 9, Shanxi beans 23.
4. the breeding method of high yielding soybeans as described in claim 1, which is characterized in that female parent uses cave when hybridization or backcrossing
It broadcasts, every cave 2-3 seeds, spacing in the rows 50-80cm, male parent uses simple grain program request, row plantation between male parent and female parent.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811139624.6A CN109042309A (en) | 2018-09-28 | 2018-09-28 | A kind of breeding method of high yielding soybeans |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811139624.6A CN109042309A (en) | 2018-09-28 | 2018-09-28 | A kind of breeding method of high yielding soybeans |
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| Publication Number | Publication Date |
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| CN109042309A true CN109042309A (en) | 2018-12-21 |
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| CN201811139624.6A Pending CN109042309A (en) | 2018-09-28 | 2018-09-28 | A kind of breeding method of high yielding soybeans |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112673953A (en) * | 2020-12-18 | 2021-04-20 | 安徽省寿县板桥草制工艺品有限公司 | Breeding method for new variety of high-yield mat grass |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101023735A (en) * | 2006-02-22 | 2007-08-29 | 河南省农业科学院棉花油料作物研究所 | Method for culturing soy-beam with stem exposed |
| KR20140102152A (en) * | 2013-02-13 | 2014-08-21 | 경북대학교 산학협력단 | Soybean plant with controlled fatty acid content and production method thereof |
| CN104585014A (en) * | 2014-04-01 | 2015-05-06 | 张大跃 | Improved variety soybean breeding method |
-
2018
- 2018-09-28 CN CN201811139624.6A patent/CN109042309A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101023735A (en) * | 2006-02-22 | 2007-08-29 | 河南省农业科学院棉花油料作物研究所 | Method for culturing soy-beam with stem exposed |
| KR20140102152A (en) * | 2013-02-13 | 2014-08-21 | 경북대학교 산학협력단 | Soybean plant with controlled fatty acid content and production method thereof |
| CN104585014A (en) * | 2014-04-01 | 2015-05-06 | 张大跃 | Improved variety soybean breeding method |
Non-Patent Citations (1)
| Title |
|---|
| 战明奎等: "我国大豆诱变育种概况", 《中国核科技报告》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112673953A (en) * | 2020-12-18 | 2021-04-20 | 安徽省寿县板桥草制工艺品有限公司 | Breeding method for new variety of high-yield mat grass |
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Application publication date: 20181221 |