CN106376454A - Breeding method of anti-nicosulfuron millet germplasm and application - Google Patents
Breeding method of anti-nicosulfuron millet germplasm and application Download PDFInfo
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Abstract
The invention discloses a breeding method of anti-nicosulfuron millet germplasm, belonging to the technical field of cross breeding. The breeding is performed through distant sexual hybridization before backcrossing. The breeding method comprises the following steps: (1) performing distant hybridization by adopting an anti-nicosulfuron green foxtail material as a male parent and cultivated millet as a female parent; and spraying nicosulfuron to the F1 plant seedling to obtain a hybrid plant with nicosulfuron resistance; and (2) further hybridizing the hybrid with the cultivated millet for 2-4 generations, selecting the resistant single plant and performing selfing for 2-3 generations; and screening out the seeds with agronomic trait of millet as well as nicosulfuron resistance to obtain new germplasm of anti-nicosulfuron millet. According to the breeding method disclosed by the invention, the hybridization process is simplified, and the accuracy and credibility of hybridization and backcrossing success are improved. The new germplasm of anti-nicosulfuron millet bred by the method disclosed by the invention has relatively good agronomic trait as well as nicosulfuron resistance.
Description
Technical field
The invention belongs to the technical field of crossbreeding, be related to the initiative of millet new germ plasm with breed breeding and in particular to
A kind of method for creating of anti-nicosulfuron (Nicosulfuron) millet germplasm and its application in Millet Breeding and application side
Method.Selection of the present invention, seed selection is simple, workable, and success rate of breeding is high.
Background technology
Millet itself does not have antiweed or Bar gene, but the Wild related germplasm resource of millet is very rich
Richness, has extensive hereditary variation type, and the antiweed breeding to millet has great importance (Wang Tianyu etc., 2000).
For many years, domestic and international researcher's priority is found that different resisting in millet Wild related germplasm Green foxtail natural mutation colony
Herbicide resistance gene, by hybridizing with millet, obtains anti-Atrazine, anti-trefanocide, anti-Sethoxydin, anti-imazethapyr etc. many
Kind resistance millet variety (Darmency and Pernes, 1985；Darmency and Pernes 1989；Wang Tianyu etc.,
2000；Shi Zhigang etc., 2010).
Nicosulfuron is the sulfonylurea herbicide with efficient, low toxicity, strong selectivity and low consumption, is beautiful agriculture pleasure again,
Most annual, perennial grassy weeds and broad leaved weed and nutgrass flatsedge can effectively be prevented and kill off, can be with other multiple herbicides, peaces
Full agent is used with.Register within 1996 for corn, foreign patent (CN87100436) in January, 2007 expired (Liu Minghong,
2012).In China, former dose of nicosulfuron achieves rapidly localization, and cost is greatly lowered, and yield rises rapidly.Cut
To in March, 2014, the nicosulfuron product in the term of validity of registration has 383, wherein active compound product 42, formulation products 341,
Compared to other several herbicides, medicine source is quite varied.Therefore, millet new germ plasm and the new varieties of anti-nicosulfuron are cultivated, right
Millet produces and is significant.
Content of the invention
It is an object of the invention to provide a kind of method for creating of anti-nicosulfuron millet germplasm and in Millet Breeding and production
In application process.The present invention successfully formulates out the millet germplasm of anti-nicosulfuron, available anti-on producing for cultivating
Nicosulfuron herbicide agent Millet Variety is laid a good foundation.Anti- nicosulfuron millet variety is safely and effectively applied on producing,
The major transformation that millet will be caused to produce.Manpower and financial resources can be saved in a large number, set up intensive system agricultural industry, tool
Have wide practical use, huge economic benefit and social benefit will be produced.
The present invention is to realize its purpose employed technical scheme comprise that：
The selection of anti-nicosulfuron millet germplasm, using remote edge sexual hybridization, the method seed selection being then returned again, bag
Include following steps：
(1), with anti-nicosulfuron Green foxtail material as male parent, carry out distant hybridization to cultivate millet for female parent, F1 plants
Strain Seedling Stage sprays nicosulfuron, obtains the hybrid with nicosulfuron resistance；
(2), to cultivate millet as recurrent parent, hybrid and cultivation millet are carried out 2-4 generation backcrossing, selects resistance individual plant certainly
Hand over 2-3 generation, filtering out Agronomic is millet and the seed with nicosulfuron resistance, obtains anti-nicosulfuron millet
New germ plasm.
Anti- nicosulfuron green bristlegrass material is obtained by following methods：
A, from the Green foxtail material of anti-imazethapyr, screening has the Green foxtail material of anti-nicosulfuron proterties concurrently
Material；
B, the anti-imazethapyr Green foxtail material of anti-nicosulfuron proterties is carried out the strong and weak screening of nicosulfuron resistance,
Select the material of nicosulfuron resistance, obtain anti-nicosulfuron Green foxtail material.
Male parent, female parent are hybridized by the way of sowing respectively, comprise the steps：Maternal normal plantation, line-spacing
0.6-0.8cm, male parent is planted in flowerpot, runs into the male parent with maternal flower synchronization, transplants male parent and carries out to planting of maternal area
Hybridization.The present invention control line-spacing 0.6-0.8cm just middle interval a line, be easy to centre let off fireworks basin hybridization, line-spacing be more than 0.8cm,
The flowerpot put can not simultaneously with both sides hybridization of female parent, reduce efficiency；Line-spacing is less than 0.6, spacing a bit little it has not been convenient to flowerpot
Mobile, do operation when hybridizing also more difficult.
The described nicosulfuron that sprays is 95.5% nicosulfuron active compound, and sprayed dose scope is 30-60g a.i./hm2.
Cultivate monoclonal antibody nicosulfuron strain kind using anti-nicosulfuron millet new germ plasm；New using anti-nicosulfuron millet
Germplasm cultivates the multiline variety that " monoclonal antibody nicosulfuron strain+not the homotype Sister Lines of antiweed or NIL " forms.
The cultivation of monoclonal antibody nicosulfuron strain kind：With the millet new germ plasm of anti-nicosulfuron as male parent, with not anti-weeding
The key millet parent material hybridization of agent, F1 generation sprays the pseudostationary that nicosulfuron kills not antiweed, then selfing 4-5
In generation, go out monoclonal antibody nicosulfuron Millet Variety in advanced lines final election.
The multiline variety that " monoclonal antibody nicosulfuron strain+not the homotype Sister Lines of antiweed or NIL " forms
Cultivate：With the millet new germ plasm of anti-nicosulfuron as male parent, hybridize with the key millet parent material of not antiweed, F1 generation is sprayed
Apply the pseudostationary that nicosulfuron kills not antiweed, then selfing 4-5 generation or the key millet parent with not antiweed
Material is recurrent parent, is returned 4-5 generation, goes out anti-nicosulfuron millet new lines, seed selection simultaneously and this anti-cigarette in advanced lines final election
The Sister Lines of the not anti-nicosulfuron of Sulfometuron Methyl millet new lines Comprehensive Traits identical or NIL, the two mixture, obtain
The multiline variety that " monoclonal antibody nicosulfuron strain+not the homotype Sister Lines of antiweed or NIL " forms.
In the cultivation of multiline variety that " the homotype Sister Lines of the antiweed of monoclonal antibody nicosulfuron strain+not " form, F1 generation
Spray nicosulfuron to kill not after the pseudostationary of antiweed, proceed as follows：
A, F2, F3 spray, for Seedling Stage, the paddy seedling that nicosulfuron kills not antiweed completely, ripe front selection agronomy
The excellent individual plant of shape, and the Some seeds that each individual plant that F2, F3 generation is selected harvests carry out seedling stage antiweed identification, eliminate
The individual plant of completely anti-or inwhole antiweeds, stays resistance to there is detached individual plant；
In B, F4 generation, seedling stage each generation no longer sprayed nicosulfuron later, carried out before maturation in the excellent family of economical character
Single-plant selection, the individual plant that is, full seed, pest and disease damage are little, lodging is light, and the Some seeds that each individual plant is harvested carry out seedling stage
Antiweed identification, selects anti-and not anti-nicosulfuron individual plant, and the next generation will resist except the strain of nicosulfuron, not anti-nicosulfuron
System carries out contrast and selects, until selecting, each proterties is stable, Comprehensive Traits identical anti-nicosulfuron millet new lines, not anti-cigarette
The Sister Lines of Sulfometuron Methyl.
In the cultivation of multiline variety that " NIL of the antiweed of monoclonal antibody nicosulfuron strain+not " forms, F1 generation
Spray nicosulfuron to kill not after the pseudostationary of antiweed, proceed as follows：
A, with the key millet parent material of not antiweed as recurrent parent, be returned 4-5 generation, per generation backcrossing all in seedling stage
Spray nicosulfuron；
Economical character elite seedling is selected, that is, full seed, pest and disease damage be little, lodging when B, last backcross population are ripe
Light individual plant, then selfing 2-3 generation, therefrom select the millet new lines of anti-nicosulfuron, select Comprehensive Traits identical simultaneously
The NIL of not anti-nicosulfuron, the two mixture forms multiline variety.
The invention has the beneficial effects as follows：The present invention utilizes the method for sexual hybridization and backcrossing by the phonetic sulphur of anti-cigarette of Green foxtail
Grand gene transfer, in cultivation millet, formulates out the Millet Breeding new germ plasm of anti-nicosulfuron, for cultivating the paddy of anti-nicosulfuron
Sub- new varieties establish material foundation.The anti-nicosulfuron millet new germ plasm economical character that the method for the present invention selects is preferable, tool
There is nicosulfuron resistance.The selection hybridization procedures of the present invention simplify, and improve hybridization, be returned the successfully degree of accuracy, confidence level.
Male parent, female parent are hybridized by the way of sowing respectively, and hybridization success rate is high, solves male parent grain weight less
And the problem of male parent, maternal flowering asynchronism.
Specific embodiment
With reference to specific embodiment, the present invention is further illustrated.
Embodiment 1
1st, the acquisition of anti-nicosulfuron Green foxtail material：
2006, Grain Institute, Provincial Academy of Agriculture and Forestry, Hebei Provinee was from your Er Fu university (University of Canadian
Guelph, Canada) introduce the Green foxtail material (Setaria viridis) of anti-imazethapyr (Imazethapyr).
Filter out the material having anti-nicosulfuron proterties concurrently within 2011 from the Green foxtail material of anti-imazethapyr；Will
The anti-imazethapyr Green foxtail material of anti-nicosulfuron proterties carries out the strong and weak screening of nicosulfuron resistance, selects the phonetic sulphur of anti-cigarette
The strong material of grand proterties, obtains anti-nicosulfuron Green foxtail material, is named as anti-nicosulfuron green bristlegrass 1S herein.
2nd, preparing hybrid combination：
Winter in 2011 and spring in 2012, are maternal in greenhouse with paddy 12 of weighing, with the Green foxtail 1S of anti-nicosulfuron
Combine 15 for male parent preparing hybrid.
3rd, the acquisition of anti-nicosulfuron millet germplasm：
Summer in 2012, filter out F1 generation anti-nicosulfuron material 10.Simultaneously with cultivation millet variety (Ji Gu 31, Ji
Paddy 32, Jin Gu 1, Henan paddy 18) hybridization (, winter in 2012 obtains F1 generation, and 3 leaf phases sprayed nicosulfuron and leave the miscellaneous of antiweed
Kind, continue and key millet variety backcrossing；The spring F2 3 leaf phases of generation in 2013 spray nicosulfuron, leave the hybrid of antiweed,
Elite seedling is selected to continue and key millet variety backcrossing, the summer F3 3 leaf phases of generation in 2013 spray nicosulfuron, carry out before maturation
Substantial amounts of Single-plant selection, and the Some seeds that each individual plant is harvested carry out seedling stage antiweed identification, select anti-nicosulfuron
Individual plant, through winter in 2013, generation spring two in 2014, summer in 2014 filter out economical character preferably, there is nicosulfuron
The millet new germ plasm 14H06 of resistance.This germplasm plant height 115.6cm, 86 days breeding times, economical character preferably, has nicosulfuron
Resistance, the Millet Variety for cultivating anti-nicosulfuron has established material foundation.
14H06 derives from combination " Henan paddy 18 × (weighing apparatus paddy 12 × anti-nicosulfuron green bristlegrass 1S) ".Wherein, Henan paddy 18 is river
The Millet Variety of southern Anyang academy of agricultural sciences's seed selection, weighing apparatus paddy 12 is precocious millet variety, and anti-nicosulfuron green bristlegrass 1S is from drawing
The material of the better resistance filtering out in the anti-imazethapyr green bristlegrass material entering.
Embodiment 2, hybridization technique
Anti- nicosulfuron green bristlegrass material 1S breeding time is too short, plant short and small it is not easy to millet hybridization.Traditional method
It is interval sowing.But for anti-nicosulfuron material, breeding time is too short, and the florescence is not easy to run into, and grain weight is limited, no
It is easy to design excessive gradient；And plant is short and small, more difficult in operation when hybridizing with millet.By repeatedly groping, we adopt
Take with anti-nicosulfuron green bristlegrass material as male parent, with key millet variety as female parent, the method that male parent, female parent are sowed respectively：
Different millet varieties make female parent, normally in field planting, male parent point different times precision drilling in flowerpot, and run into flower synchronization
Female parent, male parent flowerpot is moved near female parent, suitable distance is reserved according to the maternal plant height with male parent, in order to female parent
Bending is hybridized with male parent.The method hybridization success rate is higher, has stronger operability.
Embodiment 3, the application of anti-nicosulfuron Millet Variety
Anti- nicosulfuron millet new germ plasm can apply to the following aspects：
(1) anti-nicosulfuron millet germplasm is utilized to cultivate monoclonal antibody nicosulfuron kind：Millet novel species with anti-nicosulfuron
Matter is male parent, hybridizes with the key millet parent material of not antiweed, and F1 generation sprays nicosulfuron and kills not antiweed
Pseudostationary, in then selfing 4-5 generation, goes out monoclonal antibody nicosulfuron Millet Variety in advanced lines final election.Wherein, between precision drilling not
Seedling, suitable application rate (kg/hm2Suitable (ten thousand plants/hm of the density of reserved seedlings of)=local2) × kind mass of 1000 kernel ÷ germination percentage ÷ emergence rate
÷ survival rate of seedlings ÷ 100, the 2-5 leaf phase sprays nicosulfuron, can kill weeds；
(2) anti-nicosulfuron millet new germ plasm is utilized to cultivate monoclonal antibody nicosulfuron strain kind；Using anti-nicosulfuron paddy
Sub- new germ plasm cultivates the polyphyly that " the homotype Sister Lines (or NIL) of the antiweed of monoclonal antibody nicosulfuron strain+not " form
Kind.
The cultivation of monoclonal antibody nicosulfuron strain kind：With the millet new germ plasm of anti-nicosulfuron as male parent, with not anti-weeding
The key millet parent material hybridization of agent, F1 generation sprays the pseudostationary that nicosulfuron kills not antiweed, then selfing 4-5
In generation, go out monoclonal antibody nicosulfuron Millet Variety in advanced lines final election, may be directly applied to produce, and using nicosulfuron
Learn weeding.
The multiline variety that " the homotype Sister Lines (or NIL) of the antiweed of monoclonal antibody nicosulfuron strain+not " form
Cultivation：With the millet new germ plasm of anti-nicosulfuron as male parent, hybridize with the key millet parent material of not antiweed, F1 generation
Spray the pseudostationary that nicosulfuron kills not antiweed, then selfing 4-5 generation is (or close with the key millet of not antiweed
This material is recurrent parent, is returned 4-5 generation), the anti-nicosulfuron millet new lines of objective trait are gone out to meet in advanced lines final election,
The Sister Lines (or NIL) of the not anti-nicosulfuron basically identical with this kind Comprehensive Traits of seed selection simultaneously, the two presses one
Certainty ratio mixture (such as 1: 1), obtain " the homotype Sister Lines of the antiweed of monoclonal antibody nicosulfuron strain+not (or near isogene
System) " multiline variety that forms.
The multiline variety that " anti-nicosulfuron strain+not antiweed homotype Sister Lines " forms is to be obtained using following methods
?：
A, F2, F3 spray, for Seedling Stage, the paddy seedling that nicosulfuron kills not antiweed completely, the excellent list of ripe front selection
Strain, and the Some seeds that F2, F3 each individual plant of selecting of generation is harvested carry out seedling stage antiweed identification, eliminate do not resist completely or
All the individual plant of antiweed, stays resistance to there is detached individual plant；
B, F4 generation seedling stage each generation no longer herbicide spraying later, carries out substantial amounts of individual plant before maturation in excellent family
Select, and the Some seeds that each individual plant is harvested carry out seedling stage antiweed identification, select anti-and not anti-nicosulfuron list
Strain, the next generation is by antiweed, the strain of antiweed does not carry out contrast selection, until it is stable, comprehensive to select each proterties
The Sister Lines of the basically identical antiweed of shape, not antiweed.
The multiline variety that " anti-nicosulfuron strain+not antiweed NIL " forms is to be obtained using following methods
?：
A, with the millet new germ plasm of anti-nicosulfuron as male parent, key millet parent material hybridization with not antiweed,
F1 generation sprays the pseudostationary that nicosulfuron kills not antiweed, with the key millet parent material of not antiweed for samsara parent
This, be returned 4-5 generation, and per generation backcrossing is all in seedling spraying nicosulfuron.
B, last backcross population select fine individual plant when ripe in a large number, then selfing 2 generation, therefrom select anti-nicosulfuron
Millet new lines, select the NIL of the basically identical not anti-nicosulfuron of Comprehensive Traits, the two presses certain simultaneously
Ratio mixture forms multiline variety.
Wherein, semi-accuracy seeding, the two application rate ratio 1：(1-1.5), wherein, anti-nicosulfuron strain application rate (kg/
hm2Suitable (ten thousand plants/hm of the density of reserved seedlings of)=local2) × kind mass of 1000 kernel ÷ germination percentage ÷ emergence rate ÷ survival rate of seedlings ÷ 100,2-5
The leaf phase sprays homotype Sister Lines or the NIL that nicosulfuron kills not antiweed, realizes chemical thinning, and kills miscellaneous
Grass.
Embodiment 4
The resistant analysis of antiweed nicosulfuron：
(1) present invention anti-nicosulfuron millet germplasm genetics of resistance is stable, and inbreeding of more generation resistance level does not change.
(2) anti-sense material hybridization, first-filial generation shows antiweed, and F2 generation meets 3:1 anti-sense separates, and first backcross generation accords with
Close 1:1 anti-sense separates.Just giving reciprocal cross result consistent.Illustrate millet anti-nicosulfuron proterties by a pair of core dominant gene control
System.
Embodiment 5
Second effect analysis of anti-nicosulfuron millet germplasm resistant gene：
(1) at different densities (30 plants/square metre, 50 plants/square metre), three kinds of different modes plant anti-sense NIL
(100% anti-nicosulfuron colony, 50% resistance, 50% perceptual population mixture, 100% perceptual colony), 5 repetitions, often little
15 square metres of area；
(2) different times are investigated to economical character, by comparing different groups, different condition anti-sense NIL
Result；
(3) result of study shows that millet obtains antiweed nicosulfuron gene and will not reduce economical character level effect.
The selection of the present invention is by the base of the antiweed nicosulfuron in Green foxtail by distant hybridization method
Because importing to cultigen millet, then resistance is obtained by backcrossing and reach realistic scale, inheritance stability, good the resisting of economical character
Nicosulfuron herbicide agent millet new germ plasm, belongs to non-transgenic means, enriches further and imports external source desirable genes to millet
Technology path.
The present invention adopts distant hybridization method to formulate anti-nicosulfuron herbicide agent millet new germ plasm, is not belonging to transgenosis model
Farmland, enriches the technology path importing external source desirable genes to millet further；Simultaneously it is proposed that cultivating anti-sense nicosulfuron elder sister
Younger sister system forms multiline variety, realizes millet chemistry thinning it is achieved that the efficient profit of anti-herbicide gene while chemical weed control
With.
The present invention, by the way of Parent hybridization, has the advantage that,
(1) maternal normal plantation, line-spacing 0.6-0.8cm, male parent is planted in flowerpot, runs into the father with maternal flower synchronization
This, transplant male parent and hybridized to planting of maternal area.
Advantage：
A, male parent are anti-nicosulfuron green bristlegrass materials, breeding time short it is not easy to maternal flower synchronization, its kind is being spent
In basin, and by maternal a large amount of plantation in the ground, being easy to male parent flexibly moves, and runs into florescence suitably female parent and moves to appropriate area and does
Hybridization；
B, paternal plant are short and small, can increase its height using flowerpot, if or not, thing can be padded under flowerpot；
C, male parent grain weight are little, are so conducive to saving grain weight.
(2) by male parent interlacing kind in the ground, plant female parent by stages, in a large number in other region, run into the father of flower synchronization
Female parent, transplants female parent to male parent interlacing region.
Advantage：
, in field planting, growing way is good for A, male parent, and survival rate is high.
B, alternative female parent scope are big, and male parent utilization rate is high.
Claims (8)
1. the selection of anti-nicosulfuron millet germplasm, using remote edge sexual hybridization, the method seed selection being then returned again, it is special
Levy and be, comprise the following steps：
(1), with anti-nicosulfuron Green foxtail material as male parent, distant hybridization, F1 plant children are carried out to cultivate millet for female parent
Seedling spraying nicosulfuron, obtains the hybrid with nicosulfuron resistance；
(2), hybrid and cultivation millet are carried out 2-4 generation hybridization, select resistance individual plant selfing 2-3 generation, filter out economical character table
It is now millet and the seed with nicosulfuron resistance, obtain anti-nicosulfuron millet new germ plasm.
2. anti-nicosulfuron millet germplasm according to claim 1 selection it is characterised in that：Anti- nicosulfuron dog
Tail grass material is obtained by following methods：
A, from the Green foxtail material of anti-imazethapyr, screening has the Green foxtail material of anti-nicosulfuron proterties concurrently；
B, the anti-imazethapyr Green foxtail material of anti-nicosulfuron proterties is carried out the strong and weak screening of nicosulfuron resistance, select
The material of nicosulfuron resistance, obtains anti-nicosulfuron Green foxtail material.
3. the selection of anti-nicosulfuron millet germplasm according to claim 1 is it is characterised in that male parent, female parent are adopted
Hybridized with the mode sowed respectively, comprised the steps：Maternal normal plantation, line-spacing 0.6-0.8cm, male parent is planted in flower
In basin, run into the male parent with maternal flower synchronization, transplant male parent and hybridized to planting of maternal area.
4. a kind of application of anti-as claimed in claim 1 nicosulfuron millet germplasm it is characterised in that
Cultivate monoclonal antibody nicosulfuron strain kind using anti-nicosulfuron millet new germ plasm；Using anti-nicosulfuron millet new germ plasm
Cultivate the multiline variety that " monoclonal antibody nicosulfuron strain+not the homotype Sister Lines of antiweed or NIL " forms.
5. the application of anti-nicosulfuron millet germplasm according to claim 4 is it is characterised in that monoclonal antibody nicosulfuron strain
The cultivation of kind：With the millet new germ plasm of anti-nicosulfuron as male parent, hybridize with the key millet parent material of not antiweed,
F1 generation sprays the pseudostationary that nicosulfuron kills not antiweed, then selfing 4-5 generation, goes out the phonetic sulphur of monoclonal antibody cigarette in advanced lines final election
Grand Millet Variety.
6. the application of anti-nicosulfuron millet germplasm according to claim 4 is it is characterised in that " monoclonal antibody nicosulfuron product
The cultivation of the multiline variety that system+not the homotype Sister Lines of antiweed or NIL " forms：Millet with anti-nicosulfuron
New germ plasm is male parent, the key millet parent material hybridization with not antiweed, and F1 generation sprays nicosulfuron and kills not anti-weeding
The pseudostationary of agent, then selfing 4-5 generation or with the key millet parent material of not antiweed as recurrent parent, backcrossing 4-5
In generation, go out anti-nicosulfuron millet new lines in advanced lines final election, seed selection simultaneously is comprehensive with this anti-nicosulfuron millet new lines
The Sister Lines of the not anti-nicosulfuron of shape identical or NIL, the two mixture, obtain " monoclonal antibody nicosulfuron strain+do not resist
The multiline variety that the homotype Sister Lines of herbicide or NIL " forms.
7. the application of anti-nicosulfuron millet germplasm according to claim 6 is it is characterised in that " monoclonal antibody nicosulfuron product
In the cultivation of multiline variety that the homotype Sister Lines of system+not antiweed " form, F1 generation sprays nicosulfuron and kills not anti-weeding
After the pseudostationary of agent, proceed as follows：
A, F2, F3 spray, for Seedling Stage, the paddy seedling that nicosulfuron kills not antiweed completely, ripe front selection full seed
Individual plant, and the Some seeds that F2, F3 each individual plant of selecting of generation is harvested carry out seedling stage antiweed identification, eliminate completely not
The individual plant of anti-or whole antiweeds, stays resistance to there is detached individual plant；
In B, F4 generation, seedling stage each generation no longer sprayed nicosulfuron later, carries out Single-plant selection, and each individual plant is harvested before maturation
Some seeds carry out seedling stage antiweed identification, select anti-and not anti-nicosulfuron individual plant, of future generation by phonetic for anti-smoke abatement sulphur
The strain of grand, not anti-nicosulfuron carries out contrast and selects, until selecting, each proterties is stable, the phonetic sulphur of the anti-cigarette of Comprehensive Traits identical
Grand millet new lines, the Sister Lines of not anti-nicosulfuron.
8. the application of anti-nicosulfuron millet germplasm according to claim 6 is it is characterised in that " monoclonal antibody nicosulfuron product
In the cultivation of multiline variety that the NIL of system+not antiweed " forms, F1 generation sprays nicosulfuron and kills not anti-weeding
After the pseudostationary of agent, proceed as follows：
A, with the key millet parent material of not antiweed as recurrent parent, be returned 4-5 generation, per generation backcrossing all in seedling spraying
Nicosulfuron；
The individual plant of full seed is selected when B, last backcross population are ripe, then selfing 2-3 generation, therefrom select anti-nicosulfuron
Millet new lines, select the NIL of the not anti-nicosulfuron of Comprehensive Traits identical, the two mixture forms many simultaneously
It is kind.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106857128A (en) * | 2017-02-16 | 2017-06-20 | 贵州红四方农业发展股份有限公司 | A kind of hybrid rice male parent rice transplanting method |
| CN108575735A (en) * | 2018-07-19 | 2018-09-28 | 安阳市农业科学院 | A kind of breeding method of rapidly and efficiently selection and breeding eurytopicity millet variety |
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| CN1586159A (en) * | 2004-08-13 | 2005-03-02 | 河北省农林科学院谷子研究所 | Simplifying cultivating millet variety breeding and its cultivating method |
| CN105706917A (en) * | 2016-03-30 | 2016-06-29 | 河北省农林科学院谷子研究所 | Breeding method of special foxtail millet for forage grass |
| CN105861734A (en) * | 2016-06-17 | 2016-08-17 | 河北省农林科学院谷子研究所 | Rapid identification method for homozygosis, heterozygosis and resistance-free performance of anti-nicosulfuron genes of foxtail millet |
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2016
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| CN1586159A (en) * | 2004-08-13 | 2005-03-02 | 河北省农林科学院谷子研究所 | Simplifying cultivating millet variety breeding and its cultivating method |
| CN105706917A (en) * | 2016-03-30 | 2016-06-29 | 河北省农林科学院谷子研究所 | Breeding method of special foxtail millet for forage grass |
| CN105861734A (en) * | 2016-06-17 | 2016-08-17 | 河北省农林科学院谷子研究所 | Rapid identification method for homozygosis, heterozygosis and resistance-free performance of anti-nicosulfuron genes of foxtail millet |
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| D.S. VOLENBERG ET AL.: "Green foxtail (Setaria viridis) resistance to acetolactate synthase inhibitors", 《PHYTOPROTECTION》 * |
| 王天宇等: "抗除草剂谷子新种质的创制、鉴定与利用", 《中国农业科技导报》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106857128A (en) * | 2017-02-16 | 2017-06-20 | 贵州红四方农业发展股份有限公司 | A kind of hybrid rice male parent rice transplanting method |
| CN108575735A (en) * | 2018-07-19 | 2018-09-28 | 安阳市农业科学院 | A kind of breeding method of rapidly and efficiently selection and breeding eurytopicity millet variety |
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| CN106376454B (en) | 2018-12-25 |
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