CN102124943A - Method for producing hybrid cotton seeds by inducing male sterility of cotton with chemical male gametocide - Google Patents
Method for producing hybrid cotton seeds by inducing male sterility of cotton with chemical male gametocide Download PDFInfo
- Publication number
- CN102124943A CN102124943A CN2010105613070A CN201010561307A CN102124943A CN 102124943 A CN102124943 A CN 102124943A CN 2010105613070 A CN2010105613070 A CN 2010105613070A CN 201010561307 A CN201010561307 A CN 201010561307A CN 102124943 A CN102124943 A CN 102124943A
- Authority
- CN
- China
- Prior art keywords
- cotton
- male
- acid
- hybrid
- chemical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a method for producing hybrid cotton seeds by inducing the male sterility of cotton with a chemical male gametocide, comprising the following steps of: (1) screening the chemical male gametocide, which has a thorough castration performance and a stable effect, and cotton varieties or strains which correspond to the chemical male gametocide; (2) backcrossing for 4-6 generations by using varieties with genes to be transformed and the screened cotton varieties, spraying each backcrossed generation by using the screened chemical male gametocide, and selecting plants, which are subjected to thorough castration and free from influences harmful to the growth and the development of cotton plants, from a colony to establish a plant line till the plant line in accordance with a transformed object is selected, wherein the selected plant line is used for producing the hybrid cotton seeds after being propagated. The method provided by the invention has a good castration effect and is beneficial to cost and labor saving; the chemical male gametocide has thorough and stable effect; and the production cost of the hybrid cotton seeds is reduced by about 30 percent compared with that of artificial castration and pollination.
Description
Technical field
The invention belongs to the cotton seeds production method, be applicable on the Cotton Production to utilize intervarietal hybrid (F in the upland cotton kind
1Generation) advantage is the hybrid cotton seed production of purpose.
Background technology
Upland cotton, sea-island cotton, Asian cotton and four cultivated speciess of cotton are arranged in the Gossypium.Cotton in China is produced and is gone up the cotton seed of using at present, and except that southern Xinjiang had sea-island cotton, all the other were upland cotton.Over past ten years, Cotton in China is produced and is gone up to utilize intervarietal hybrid (F in the upland cotton kind
1Generation) advantage is that the hybrid cotton area of purpose enlarges rapidly, accounts for 10.9% of national acreage under cotton by 1999, develop into 2008 45%, be the cotton region, the Yangtze river basin of main planting patterns especially with seedling raising and transplanting, the hybrid cotton area accounts for acreage under cotton more than 80%.With live be the landlocked cotton region in the Huanghe valley of main planting patterns and northwest, the hybrid cotton area also is very fast developing state.China has become maximum in the world hybrid cotton plantation state.Domestic hybrid cotton seed production more than 98% is the method that adopts the artificial emasculation pollination, this method has been spent hero by pursuing by hand, has been pollinated by flower, not only time-consuming taking a lot of work, and seed production cost height, cause commercial seed on the high side, price general charged is at 120 ~ 140 yuan/kg, and conventional variety commercial seed price is at 8 ~ 9 yuan/kg, and this has become " bottleneck " that the restriction hybrid cotton further develops.
Summary of the invention
The problem that this invention exists at artificial emasculation pollination's method, by male sterilant inducing cotton stamen sterile (hereinafter to be referred as " chemical emasculation "), reach the castration purpose, both can remove the link of artificial emasculation in the hybrid cotton seed production process from, can realize killing thoroughly male again, the seed quality of producing meets Ministry of Agriculture's " hybrid cotton corresponding seed quality criteria ", and to reducing seed production cost and selling price, promoting China's hybrid cotton sustainable development has positive effect.
The different formulations that the inventor utilizes existing various crop male sterilant is found after the repetition test having carried out on the cotton: (1) same cotton variety is different to the reaction of different chemical male gametocide; Different cotton varieties also are different to the reaction of same male sterilant.This means between cotton variety (being) and the male sterilant to have selectivity, utilize existing male sterilant can find the thorough and stable cotton variety of gametocidal effect (being).(2) the chemical emasculation effect is heritable, be subjected to a pair of Gene Handling, belong to qualitative character heredity, so adopt the method for backcrossing, can be in the female parent of required production hybrid cotton seed kind, the strain of heterosis utilization target (or meet) with the gene transformation of control chemical emasculation effect, thus time and the cost that medicament screens reduced.
The technical scheme of employing of the present invention is that the male sterile hybrid cotton seed of a kind of male sterilant inducing cotton production method comprises the steps:
(1) male sterilant and the corresponding with it cotton variety or the strain of thoroughly male and effect stability killed in screening; The cotton variety that filters out or strain as the cotton variety that can be used for the chemical emasculation production of hybrid seeds, are designated as kind A; With the corresponding male sterilant called after of A kind CH;
(2) the gene backcross transformation of control chemical emasculation effect: P need be allowed kind A and P backcross 4-6 generation by the kind of transformation gene; Backcross from generation to generation for each, CH sprays with male sterilant, selects gametocidal effect thoroughly and to growth rate of cotton plant to grow the plant that has no adverse effects from colony, sets up strain system, until selecting the strain system that meets the transformation target, after the expansion of roguing system is numerous, be used for the production of hybrid cotton seed.
The inventive method was risen cotton Co., Ltd already in 2008 by Jiangsu Province section and is used for the production of hybrid cotton seed, seeding 2500kg in 2008,6000kg in 2009, put goods on the market, production practices are the result show, this method hybridizing rate reaches 100%, effect stability, the seed quality of being produced meets " the hybrid cotton corresponding seed quality criteria " that the Ministry of Agriculture issues, and more than 100kg/ mu, the comparable employing artificial emasculation pollination's of seed production cost method reduces about 30% to seeding amount (hair seed).6 of the new lines that adopts the backcross transformation method to breed to be used for the chemical emasculation seeding technique.
Compared with prior art, this invention advantage is as follows:
1, technical thought has novelty.This invention is on the basis of fully researching and analysing the relevant this respect documents and materials of forefathers, from the angle of genetic breeding but not the method that the angle research chemical emasculation of chemical emasculation novel form development is produced the hybrid cotton seed, broken through the technical thought of existing " is target to develop the new chemical male gametocide ".In addition, the clear and definite backcross transformation method that adopts can be with the gene transformation of control chemical emasculation effect in the female parent of required production hybrid cotton seed kind, the strain of heterosis utilization target (or meet), with saving medicament screening time and cost.
2, good, the abridged edition saving of labor of gametocidal effect.The chemical emasculation effect is thorough and stable, and the seed production cost reduces about 30% than the artificial emasculation pollination.
Description of drawings
Fig. 1 is a breeding screening collection of illustrative plates of the present invention.
Embodiment
(1) male gametocide and the corresponding with it cotton variety (being) thereof of thoroughly male and effect stability killed in screening
1, male sterilant: the male sterilant that uses among the present invention amounts to 16 kinds for once proof on various crop such as cotton, paddy rice, wheat and vegetables.Comprise: (1) dichloroacetic acid, (2) dichloropropionic acid, (3) chloropon, (4) dichloro sodium methacrylate, (5) Triiodobenzoic acid, (6) Dichloroethyl phosphoric acid, (7) maleic acid hydrazine, (8) dichlorphenoxyacetic acid, (9) sodium nucleinate, (10) Dichloroethyl trimethyl potassium chloride, (11) sulfamic acid, (12) are to aniline sulfonic acid, (13) diphenylamine sulfonic acid, (14) dichloropropionic acid, (15) Tianjin quinoline difficult to understand and (16) benzene pyridazine propyl ester.
2, screening technique: with above-mentioned 16 kinds of male sterilants, the concentration gradient according to 0.1% ~ 1.0% (gradient is spaced apart 0.1 percentage point) is sprayed cotton variety.Grow index such as have no adverse effects according to gametocidal effect (killing hero with whether thoroughly is standard) with to cotton growth, determine male sterilant and corresponding with it cotton variety (being) thereof.
(2) transfer method
This invention adopts gene transformation that backcross method will control gametocidal effect in the required female parent of producing the hybrid cotton seed (or meet the required kind of heterosis utilization target, be).Backcross 4 ~ 6 times.
Specific procedure following (as Fig. 1):
Setting A is to filter out the cotton variety (being) that can be used for the chemical emasculation production of hybrid seeds, and P is need be by the kind of transformation gene (being), and CH is and the corresponding male sterilant of A kind (being).
Backcross from generation to generation for each, CH sprays with male sterilant, selects gametocidal effect thoroughly and to growth rate of cotton plant to grow the plant that has no adverse effects from colony, sets up strain system, until selecting the strain system that meets the transformation target, after the expansion of roguing system is numerous, be used for the production of hybrid cotton seed.
(3) cotton variety that has filtered out (being) and corresponding chemical male gametocide prescription are as table 1
Table 1. cotton variety (being) and corresponding chemical male gametocide prescription
| Kind (being) | Key property | The chemical emasculation agent prescription |
| 1, section is cotton No. 2 | Anti-blight, sense verticillium wilt, bell is big, and product are of fine quality. | (3) 60%+(5) 40% |
| 2, section is cotton No. 4 | Pest-resistant, anti-blight, anti-verticillium wilt, yielding ability is good, and coordinate force is good, with the F of most of kind preparations 1Combination hybrid vigour is strong, and filtering out can be higher for the probability of production application combination. | （8）50%+（13）30%+(4)20% |
| 3,781 | Be utilization backcross transformation method with the new lines of screening after the control chemical emasculation gene transformation in cotton No. 4 of the section.Principal character and section cotton No. 4 identical. | Equal cotton No. 4 |
| 4,465 | With 781, but the resisting verticillium performance is better than 781. | Equal cotton No. 4 |
| 5,902 | With 781, comprehensive proterties such as yielding ability, resistance and quality is better than 781. | Equal cotton No. 4 |
| 6,538 | With 781, high anti-blight. | Equal cotton No. 4 |
| 7, Da Feng 30 | Pest-resistant, anti-blight, bell is big, coordinate force good. | (8) 20%+(16 60%+(6)) 10%+ (10) 10% |
| 8,359 | The new lines that filters out after the gene transformation of utilization backcross transformation method with control chemical emasculation among the Da Feng 30.Key property is with Da Feng 30. | With Da Feng 30 |
| 9,232 | Pest-resistant, anti-blight, sense verticillium wilt.Bell is big (7.0g) especially, ginning outturn height (42%). | (10) 60%+(1) 40% |
| 10,380 | The new lines that filters out after the gene transformation of utilization backcross transformation method with control chemical emasculation in 232 strains.Principal character is with 232, but anti-verticillium wilt. | With 232 |
| 11,470 | Comprehensive proterties such as output, quality and resistance is better, but bell (5.4g) less than normal.Coordinate force is better. | (3) 50%+(15) 50% |
| 12,630 | High anti-blight, pest-resistant, bell (5.4g) less than normal, ginning outturn higher (42%), coordinate force is better. | (8) 20%+(13 70%+(1)) 10% |
| 13,618 | High ginning outturn (45%).Pest-resistant, anti-blight, anti-verticillium wilt.Fiber quality is better. | （10）80%+（5）20% |
Annotate: male sterilant title in the digitized representation in the chemical emasculation agent prescription bracket " four, (), 1 ".
(4) spraying time and number of times
Spray date the 5th day after 10% cotton plant reveals first flower bud for the first time, medicament killed the male term of validity 10 ~ 14 days.After for the first time spraying medicine, every spray in 8 ~ 9 days once, spray altogether 4 times.If rain in 6 hours behind the spray medicine, then need after rain stops, to fill spray once.If spray medicine given time is met rain, spray date should be shifted to an earlier date, and spray medicine number of times is increased by 1 ~ 2 time.Stop the flower bud to grow after the artificial pollination, the manual removal.
Claims (2)
1. the male sterile hybrid cotton seed of a male sterilant inducing cotton production method is characterized in that, comprises the steps: that (1) screening kills male sterilant and the corresponding with it cotton variety or the strain of thoroughly male and effect stability; The cotton variety that filters out or strain as the cotton variety that can be used for the chemical emasculation production of hybrid seeds, are designated as kind A; With the corresponding male sterilant called after of A kind CH;
(2) the gene backcross transformation of control chemical emasculation effect: P need be allowed kind A and P backcross 4-6 generation by the kind of transformation gene; Backcross from generation to generation for each, CH sprays with male sterilant, selects gametocidal effect thoroughly and to growth rate of cotton plant to grow the plant that has no adverse effects from colony, sets up strain system, until selecting the strain system that meets the transformation target, after the expansion of roguing system is numerous, be used for the production of hybrid cotton seed.
2. method according to claim 1, it is characterized in that, in the step (1) the employed male sterilant of screening be dichloroacetic acid, dichloropropionic acid, chloropon, dichloro sodium methacrylate, Triiodobenzoic acid, Dichloroethyl phosphoric acid, maleic acid hydrazine, dichlorphenoxyacetic acid, sodium nucleinate, Dichloroethyl trimethyl potassium chloride, sulfamic acid, to aniline sulfonic acid, diphenylamine sulfonic acid, dichloropropionic acid, Tianjin quinoline difficult to understand and benzene pyridazine propyl ester.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105613070A CN102124943A (en) | 2010-11-26 | 2010-11-26 | Method for producing hybrid cotton seeds by inducing male sterility of cotton with chemical male gametocide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105613070A CN102124943A (en) | 2010-11-26 | 2010-11-26 | Method for producing hybrid cotton seeds by inducing male sterility of cotton with chemical male gametocide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102124943A true CN102124943A (en) | 2011-07-20 |
Family
ID=44263400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105613070A Pending CN102124943A (en) | 2010-11-26 | 2010-11-26 | Method for producing hybrid cotton seeds by inducing male sterility of cotton with chemical male gametocide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102124943A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499057A (en) * | 2011-10-26 | 2012-06-20 | 龚辉 | Cotton castration hybrid seed production method |
| CN103081798A (en) * | 2012-11-12 | 2013-05-08 | 陕西省杂交油菜研究中心 | Method for improving rape hybrid by chemically inducing male sterility |
| CN104920041A (en) * | 2015-06-25 | 2015-09-23 | 李小海 | Sunlight greenhouse pepper high-yield cultivation method |
| CN106305407A (en) * | 2016-08-19 | 2017-01-11 | 李建永 | Method for culturing rape seeds high in sterol level |
-
2010
- 2010-11-26 CN CN2010105613070A patent/CN102124943A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499057A (en) * | 2011-10-26 | 2012-06-20 | 龚辉 | Cotton castration hybrid seed production method |
| CN102499057B (en) * | 2011-10-26 | 2013-08-14 | 龚辉 | Cotton castration hybrid seed production method |
| CN103081798A (en) * | 2012-11-12 | 2013-05-08 | 陕西省杂交油菜研究中心 | Method for improving rape hybrid by chemically inducing male sterility |
| CN103081798B (en) * | 2012-11-12 | 2015-04-22 | 陕西省杂交油菜研究中心 | Method for improving rape hybrid by chemically inducing male sterility |
| CN104920041A (en) * | 2015-06-25 | 2015-09-23 | 李小海 | Sunlight greenhouse pepper high-yield cultivation method |
| CN106305407A (en) * | 2016-08-19 | 2017-01-11 | 李建永 | Method for culturing rape seeds high in sterol level |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106069720A (en) | Brassica campestris L dihaploid induction system's selection-breeding cabbage type rape variety and the method for material | |
| CN104488695A (en) | Method for cultivating 213 of new extra-early high-ginning-outturn strain | |
| Herath et al. | Anther culture performance in selected high yielding indica (of Sri Lanka) and japonica rice varieties and their inter sub-specific hybrids | |
| CN102124943A (en) | Method for producing hybrid cotton seeds by inducing male sterility of cotton with chemical male gametocide | |
| CN1826875A (en) | Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character | |
| CN105918111A (en) | Breeding method for novel japonica rice cytoplasmic sterile line | |
| CN104206268A (en) | Novel method for sensitively cultivating efficient rice intelligent sterile line by using herbicides | |
| CN105993922B (en) | A kind of Cotton Breeding Methods of cold plasma processing pollen and application | |
| CN1075346C (en) | Technique for breeding of hybridized rice | |
| CN104115754A (en) | Radish maintainer line with Ogura male-sterile cytoplasms and breeding method for radish maintainer line with Ogura male-sterile cytoplasms | |
| Devaux | Comparison of Anther Culture and the Hordeum bulbosum Method for the Production of Doubled Haploids in Winter Barley: II. Variation of Chromosome Number and Seed Set in the Progeny | |
| CN103098700A (en) | Breeding method of cotton cytoplasmic male sterile line | |
| CN115777526A (en) | Breeding method of fine grain fragrance type high-quality temperature-sensitive rice genic male sterile line | |
| CN112616651B (en) | Breeding method of glyphosate-resistant cotton genic male sterile dual-purpose line | |
| CN108935068B (en) | Method for improving fertility temperature sensitivity of brassica napus pol CMS sterile line | |
| CN103733982B (en) | A kind of selection of tomato parent pure lines of anti-tomato late blight | |
| CN1943321B (en) | Cross breeding mating group and mating planting method for round-grained nonglutinous rice in vitro ear | |
| CN100451120C (en) | Molecular breeding method of ternary cotton hybrid with insect-resisting transgene | |
| CN100475026C (en) | Hybrid-rice breeding method | |
| Panda et al. | Crossability relationship between wild cotton Gossypium armourianum and Gossypium gossypoides with American cotton | |
| CN101606484A (en) | A kind of method of utilizing colored cotton specialized character seed selection hybrid cotton | |
| JP2958457B1 (en) | Production of hybrid seeds of the first generation and production, maintenance and propagation of seed parent lines | |
| CN105309302B (en) | The seed selection of the capsicum material that CMV resistances are controlled by single recessive gene and authentication method | |
| CN105284592B (en) | A kind of method of Ogura cytoplasmic male sterility system seed selection | |
| CN109006465A (en) | A kind of new varieties HZ-148 selection of no film cotton |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110720 |