CN103098700A - Breeding method of cotton cytoplasmic male sterile line - Google Patents
Breeding method of cotton cytoplasmic male sterile line Download PDFInfo
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- CN103098700A CN103098700A CN 201210468807 CN201210468807A CN103098700A CN 103098700 A CN103098700 A CN 103098700A CN 201210468807 CN201210468807 CN 201210468807 CN 201210468807 A CN201210468807 A CN 201210468807A CN 103098700 A CN103098700 A CN 103098700A
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Abstract
The invention discloses a breeding method of a cotton cytoplasmic male sterile line. The method comprises the steps of hybridizing cotton Thurber's cotton (G.thurbtri) and upland cotton (G.hirsutum), selecting a sterile plant from filial generation; then carrying out backcross on the sterile plant, peach and an insect resistant material 6001, thereby obtaining the cotton cytoplasmic male sterile line after 12 generations of male sterile backcross transformation for at least seven years. The cotton cytoplasmic male sterile line screened and cultivated by the method disclosed by the invention is consistent in agronomic trait, stable and through in incompatibility, insect-resistant, and disease-resistant; the restorer recovery rate is 100%; the outcrossing rate is 91.2%; and the breeding method has wide development prospect and better utilization value.
Description
Technical field
The present invention relates to the cotton breeding field, particularly relate to the cytoplasm male sterility line selection that grows cotton.
Background technology
In the cotton hybrid vigor breeding, generally adopt at present the artificial emasculation pollination to produce hybrid seed both at home and abroad, utilize male sterile line preparing hybrid seed to use less in actual production.Artificial emasculation is produced hybrid seed, and time-consuming, cost is high, and seed quality is difficult to guarantee.Along with the development of economic society and rural laborer's significantly minimizing, the method for artificial emasculation more and more can not adapt to the needs of Agriculture Production.Utilize cotton sterile male line preparing hybrid seed, the time and labour saving, cost is low, and seed quality is guaranteed.This is the inevitable requirement of Cotton Production, is also the hope place of Cotton seed industry.
Use in cytoplasm male sterility line research, generally adopt the cytoplasm such as Hackney cotton, sea-island cotton, because there is obvious negative effect in its sterile cell to filial generation, and hybrid cotton that can not the strong advantage of seed selection is applied to produce, and has seriously restricted the wide application of hybrid cotton.
Summary of the invention
Main purpose of the present invention is to provide the cytoplasm male sterility line selection that grows cotton, and resulting male sterile line economical character is consistent, and sterility is stable, thoroughly.
In order to realize the foregoing invention purpose, the technical solution used in the present invention is as follows:
One aspect of the present invention relates to the cytoplasm male sterility line selection that grows cotton, and it is characterized in that comprising the steps:
(1) hybridize with cotton plucked instrument Bai Shi cotton (G.thurbtri) and upland cotton (G.hirsutum), select sterile strain from filial generation, then get F1 generation with large peach, pest-resistant material 6001 with sterile strain combo; F1 therefrom filters out sterile strain in torrid zone plantation;
(2) use in the torrid zone 6001 with sterile strain backcross to get F1B1 generation, the same year in the temperate zone or the subtropics backcross with F1B1 generation with 6001 again and obtain F1B2, then use in the torrid zone 6001 and F1B2 backcross;
(3) process of repeating step (2), the male sterile backcross transformation through 7 years 12 generations obtains the cotton cytoplasm male sterility line at least.
In a preferred embodiment of the present invention, the described torrid zone refers to Hainan, is preferably the Sanya.
In another preferred embodiment of the present invention, described temperate zone or subtropics refer to Hunan or Hubei, are preferably Yueyang.
Cultivate cotton cells matter male sterile line by implementing method screening of the present invention, its economical character is consistent, and sterility is stable, thorough, pest-resistant, disease-resistant, restorer recovery rate 100%, outcrossing seed-setting rate 91.2% has wide DEVELOPMENT PROSPECT and value preferably.
Description of drawings
Fig. 1: backcross breeding collection of illustrative plates.
Embodiment
Below in conjunction with embodiment, foregoing invention content of the present invention is described in further detail.
plucked instrument Bai Shi cotton (G.thurbtri) and upland cotton (G.hirsutum) hybridize, (sterile strain is without pollen to select sterile strain from filial generation, select from the field flowering stage), then use large peach, (this is our breeding material to pest-resistant material 6001, can be called the cotton strain) get F1 generation with sterile strain combo, F1 is for planting in the Sanya, major part is sterile strain, therefrom filter out sterile strain, use in the Sanya 6001 with sterile strain backcross to get F1B1 generation, F1B1 representative existing 100% is sterile, again with 6001 with F1B1 for backcross obtain F1B2 in Yueyang the same year, show as 100% sterile, process above repeating, use again 6001 to backcross with F1B2 in the Sanya, male sterile backcross transformation (backcross breeding collection of illustrative plates shown in Figure 1) through 7 years 12 generations, cultivate into male sterile line 6001A, reached economical character consistent, sterility is stable, thoroughly.
The male sterile line principal character characteristic of institute's seed selection
1) fertility.6001A male sterile line sterility shows as non-pollen type.In the Breeding Process in 7 years 12 generations, each field investigation from generation to generation that backcrosses all shows as 100% without pollen male sterile.Sterility is always stable, thorough.6001A to the reproductive population of strain more than 1000 carried out field investigation in 2010, sterile plant rate 100%, sterile degree 100%.Under manually isolation condition of 50 strains, self-fruitful rate is 0.1250 flowers of artificial pollination become 1140, bell, become bell rate 91.2%, 25.8, average single bell seed.
2) economical character.140-150 centimetre of male sterile line plant height, the plant turriform, looser, the few hair of cane.The first fruit branch joint position 6-7, effective 20 left and right of fruit branch, fruit branch length is medium, slightly upwards lifts.The cotyledon blade is larger, kidney shape, true leaf median size, bottle green.The corolla milky, flower pesticide is faint yellow.The long oval of bell, cotton boll 4-5 chamber.The seed oval, aterrimus is with short flannel.In sowing on Yueyang April 20, flower at the beginning of July 5, late August blow-of-cottons, the 120-125 days time of infertility.
3) flowering habit.Begin to bloom about July 5, enter full-bloom stage on July-20 on the 15th, in 8 months, the last ten-days period quantity of blooming reduce, in 9 months, still have a small amount of invalid flower the last ten-days period.In one day, fine day 6:30 in morning begins to bloom, bloom in a large number to 8:00, and the 9:00 end of blooming.Overcast and rainy morning, 8:00-9:00 began to bloom, and 11:00 is bloomed the left and right in a large number, until afternoon, 14:00-15:00 can be bloomed successively.The high temperature fine day is bloomed many, blooms concentrated, and the low temperature overcast and rainy day sky is bloomed few, longer duration.
Need to prove, be more than Breeding Process and the result of a wherein experimental field; In the concrete process of implementing of the present invention, 9 experimental field have independently been used altogether, be divided into 3 years (2002,2003,2004) annual 3 experimental field carry out seed selection successively, all successfully obtained the male sterile line of same characteristic, can find out that selection of the present invention has extraordinary repeatability and reproducibility.
Should be understood that; specific embodiments of the invention are only the purposes of property explanation presented for purpose of illustration; it limits protection scope of the present invention never in any form; those skilled in the art can be improved or conversion according to the above description, and all these improve and conversion all should belong to the protection domain of claims of the present invention.
Claims (3)
1. the cytoplasm male sterility line selection that grows cotton, is characterized in that comprising the steps:
(1) hybridize with cotton plucked instrument Bai Shi cotton (G.thurbtri) and upland cotton (G.hirsutum), select sterile strain from filial generation, then get F1 generation with large peach, pest-resistant material 6001 with sterile strain combo; F1 therefrom filters out sterile strain in torrid zone plantation;
(2) use in the torrid zone 6001 with sterile strain backcross to get F1B1 generation, the same year in the temperate zone or the subtropics backcross with F1B1 generation with 6001 again and obtain F1B2, then use in the torrid zone 6001 and F1B2 backcross;
(3) process of repeating step (2), the male sterile backcross transformation through 7 years 12 generations obtains the cotton cytoplasm male sterility line at least.
2. selection according to claim 1, the described torrid zone refers to Hainan, is preferably the Sanya.
3. selection according to claim 1, described temperate zone or subtropics refer to Hunan or Hubei, are preferably Yueyang.
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| CN 201210468807 CN103098700A (en) | 2012-11-09 | 2012-11-09 | Breeding method of cotton cytoplasmic male sterile line |
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| CN 201210468807 CN103098700A (en) | 2012-11-09 | 2012-11-09 | Breeding method of cotton cytoplasmic male sterile line |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598083A (en) * | 2013-09-11 | 2014-02-26 | 山西省农业科学院棉花研究所 | Method of synchronously breeding cotton restorer line and sterile line |
| CN106755081A (en) * | 2017-01-25 | 2017-05-31 | 广西大学 | A kind of method that cotton transgenic formulates cytoplasmic male sterile line |
| CN107950382A (en) * | 2017-12-04 | 2018-04-24 | 广西大学 | It is a kind of to utilize the method for joining core kapok initiative cotton cells matter male sterile line |
| CN107996388A (en) * | 2017-12-04 | 2018-05-08 | 广西大学 | A kind of method using freestone kapok initiative cotton cells matter male sterile line |
-
2012
- 2012-11-09 CN CN 201210468807 patent/CN103098700A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598083A (en) * | 2013-09-11 | 2014-02-26 | 山西省农业科学院棉花研究所 | Method of synchronously breeding cotton restorer line and sterile line |
| CN106755081A (en) * | 2017-01-25 | 2017-05-31 | 广西大学 | A kind of method that cotton transgenic formulates cytoplasmic male sterile line |
| CN106755081B (en) * | 2017-01-25 | 2020-06-16 | 广西大学 | Method for creating cytoplasmic male sterile line by cotton transgenosis |
| CN107950382A (en) * | 2017-12-04 | 2018-04-24 | 广西大学 | It is a kind of to utilize the method for joining core kapok initiative cotton cells matter male sterile line |
| CN107996388A (en) * | 2017-12-04 | 2018-05-08 | 广西大学 | A kind of method using freestone kapok initiative cotton cells matter male sterile line |
| CN107996388B (en) * | 2017-12-04 | 2021-03-30 | 广西大学 | Method for creating cotton cytoplasmic male sterile line by using karyorrhoea batal |
| CN107950382B (en) * | 2017-12-04 | 2021-03-30 | 广西大学 | Method for creating cotton cytoplasmic male sterile line by using binuclear kapok |
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Owner name: HUNAN DONGTING GAOKE SEED INDUSTRY CO., LTD. Effective date: 20130701 |
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Effective date of registration: 20130701 Address after: Hunan province Yueyang City Banqiao road 414000 No. 452 Applicant after: Yueyang Institute of Agricultural Sciences Applicant after: Hunan Dongting hi tech seed industry Limited by Share Ltd Address before: Hunan province Yueyang City Banqiao road 414000 No. 452 Applicant before: Yueyang Institute of Agricultural Sciences |
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Application publication date: 20130515 |