CN105918111A - Breeding method for novel japonica rice cytoplasmic sterile line - Google Patents
Breeding method for novel japonica rice cytoplasmic sterile line Download PDFInfo
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- CN105918111A CN105918111A CN201610310300.9A CN201610310300A CN105918111A CN 105918111 A CN105918111 A CN 105918111A CN 201610310300 A CN201610310300 A CN 201610310300A CN 105918111 A CN105918111 A CN 105918111A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a breeding method for a novel japonica rice cytoplasmic sterile line. The breeding method includes the steps that 1, a japonica rice maintainer line with good comprehensive characters is bred; 2, an Indonesia paddy field valley type cytoplasmic indica sterile line (such as quan 9311A, zhong 9A and II-32A) hybridizes with the japonica rice maintainer line to harvest sterile line seeds; 3, the japonica rice maintainer line serves as recurrent parent and continuously backcrosses with the sterile line seeds until characters of the sterile line are basically consistent with those of the japonica rice maintainer line, and the sterile line is stable and regular; 4, the stable and uniform Indonesia paddy field valley type cytoplasmic japonica rice sterile line is used for hybridizing with an Indica restoring line, and a good Indica-japonica hybrid rice combination is bred. According to the breeding method for the novel japonica rice cytoplasmic sterile line, the bred Indonesia paddy field valley type cytoplasmic indica sterile line is stable in fertility and free of self-fruitfulness and is not affected by high-temperature environment, and the good Indica-japonica hybrid rice combination usable in production can be obtained through matching.
Description
Technical field
The present invention relates to Oryza sativa L. breeding technique field, particularly relate to the selection of a kind of novel japonica rice cytoplasmic male sterile line.
Background technology
The most domestic three is that japonica rice sterile substantially has three classes by Cytoplasm division, and one is the Yunnan type Cytoplasm of Yunnan Prov Agriculture University Li Zheng friend invention.Two is the BT type Cytoplasm invented by Japan university of Ryukyu ShinjyoC and O.mura of domestic introduction.This two classes Cytoplasm japonica rice sterile large-area applications the most at home, allot the superior hybrid crosses japonica rice combination used in multiple production or Hybrid Rice Combination handed over by Xian round-grained rice, but shortcoming is sterile line fertility instability, can be produced self-fertility by Effect of Hyperthermic Environment, causes seed purity defective.Three is the Yebai cytoplasmic japonica rice sterile of the institute of agricultural sciences of Guangde County of Anhui Province selection-breeding, this sterile line stable fertility, is not had self-fertility by Effect of Hyperthermic Environment, and seed purity is guaranteed, but can not find extensive source (restorer) and Hybrid Rice Combination cannot be allotted, produce and cannot use.The novel japonica rice cytoplasmic male sterile line of selection-breeding of the present invention, not only stable fertility, be there is no self-fertility phenomenon by Effect of Hyperthermic Environment, and combo can go out to produce the upper excellent Xian round-grained rice friendship Hybrid Rice Combination that can utilize.
Summary of the invention
The invention aims to solve shortcoming present in prior art, and the selection of a kind of novel japonica rice cytoplasmic male sterile line proposed.
To achieve these goals, present invention employs following technical scheme:
A kind of selection of novel japonica rice cytoplasmic male sterile line, the selection of this novel japonica rice cytoplasmic male sterile line: utilize Indonesia's paddy field paddy type Cytoplasm indica cms line and japonica rice to keep system's hybridization, backcross through many generations, select Indonesia's paddy field paddy type Cytoplasm japonica rice sterile line；
Specifically comprise the following steps that
The excellent japonica rice of S1: selection-breeding Comprehensive Traits keeps system, japonica rice strain round-grained rice 9523(force fortune round-grained rice No. 7/round-grained rice 12-5 with yield is high, strain Leaf pattern is good) it is maternal, it is paternal hybrid with Japan's western light of japonica rice variety, with round-grained rice 9523/western light as female parent, doing paternal hybrid with blast resisting material round-grained rice 01-2, adding is commissioned to train educates；
S2: keep system hybridize and gather in the crops male-sterile seed with japonica rice with Indonesia paddy field paddy type Cytoplasm indica cms line (such as an aromatic plant metioned in ancient books 9311A, middle 9A, II-32A etc.), selecting the individual plant that plant type is good, meter Zhi Hao, disease resistance are strong, setting percentage is high, stigma exposing ratio is high in the F7 cultivated in S1 is that male parent hybridizes with an aromatic plant metioned in ancient books 9311A；
S3: keep system as recurrent parent and male-sterile seed continuous backcross using japonica rice, be returned to sterile line and keep being that character is basically identical with japonica rice, and stable neat；
S4: hybridize with indica restorer line with Indonesia's paddy field paddy type Cytoplasm japonica rice sterile of stable and consistent, selects excellent Xian round-grained rice and hands over Hybrid Rice Combination.
Preferably, the rice cultivating place in described step S1 is Hainan and Anhui.
Preferably, adding, in described step S2 and S3, the cultivation algebraically educated of being commissioned to train is 6-10 generation.
The selection of a kind of novel japonica rice cytoplasmic male sterile line that the present invention provides, Indonesia's paddy field paddy Cytoplasm indica cms line and japonica rice is utilized to keep system's hybridization, and gather in the crops male-sterile seed, select excellent Xian round-grained rice and hand over Hybrid Rice Combination, Indonesia's paddy field paddy Cytoplasm japonica rice sterile line of selection-breeding, not only stable fertility, is not had self-fertility phenomenon by Effect of Hyperthermic Environment, and combo can go out to produce the upper excellent Xian round-grained rice friendship Hybrid Rice Combination that can utilize.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with specific embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1
A kind of selection of novel japonica rice cytoplasmic male sterile line, the selection of this novel japonica rice cytoplasmic male sterile line: utilize Indonesia's paddy field paddy type Cytoplasm indica cms line and japonica rice to keep system's hybridization, backcross through many generations, select Indonesia's paddy field paddy type Cytoplasm japonica rice sterile line；
Specifically comprise the following steps that
The excellent japonica rice of S1: selection-breeding Comprehensive Traits keeps system, japonica rice strain round-grained rice 9523(force fortune round-grained rice No. 7/round-grained rice 12-5 with yield is high, strain Leaf pattern is good) it is maternal, it is paternal hybrid with Japan's western light of japonica rice variety, with round-grained rice 9523/western light as female parent, doing paternal hybrid with blast resisting material round-grained rice 01-2, adding is commissioned to train educates；
S2: keep system hybridize and gather in the crops male-sterile seed with japonica rice with Indonesia paddy field paddy type Cytoplasm indica cms line (such as an aromatic plant metioned in ancient books 9311A, middle 9A, II-32A etc.), selecting the individual plant that plant type is good, meter Zhi Hao, disease resistance are strong, setting percentage is high, stigma exposing ratio is high in the F7 cultivated in S1 is that male parent hybridizes with an aromatic plant metioned in ancient books 9311A；
S3: keep system as recurrent parent and male-sterile seed continuous backcross using japonica rice, be returned to sterile line and keep being that character is basically identical with japonica rice, and stable neat.
S4: hybridize with indica restorer line with Indonesia's paddy field paddy type Cytoplasm japonica rice sterile of stable and consistent, selects excellent Xian round-grained rice and hands over Hybrid Rice Combination；
Further, the rice cultivating place in described step S1 is Hainan and Anhui, and the cultivation algebraically that adding in described step S2 and S3 is commissioned to train educates is 6-10 generation.
The concrete cultivation flow process of the selection of this novel japonica rice cytoplasmic male sterile line is as follows:
The japonica rice strain round-grained rice 9523(force fortune round-grained rice No. 7/round-grained rice 12-5 that First Year winter-Second Year Hainan in spring yield is high, strain Leaf pattern is good) it is maternal, it is paternal hybrid with Japan's western light of japonica rice variety.
Second Year positive season plantation round-grained rice 9523/western light, and with round-grained rice 9523/western light as female parent, do paternal hybrid with blast resisting material round-grained rice 01-2.
-the three year spring of Second Year Dong adds generation to Hainan.
Separating plantation 1800 strains 3rd year positive season F2, autumn is selected precocious, plant type is good, fringe is big, setting percentage is high, proper height, stigma exposing ratio height individual plant 18.
-the four year spring of 3rd year Dong adds generation at Hainan plantation F3, and plant type is good, setting percentage is high, stigma exposing ratio height individual plant 25 for results.
4th year positive season plantation F4, the later stage eliminates that ripe color is undesirable, rice matter difference and the strain of not blast resisting, finally retains 22 individual plants.
-the five year spring Hainan F5 of 4th year Dong adds generation, and plant type is good, meter Zhi Hao, disease resistance are strong, setting percentage is high, stigma exposing ratio height individual plant 17 in selection.
5th year positive season plantation F6, plant type is good, setting percentage is high, meter Zhi Hao, stigma exposing ratio height individual plant 6 in selection.
-the six year spring of 5th year Dong plants F7 in Hainan.Character stable and consistent.Plant type is good, meter Zhi Hao, disease resistance are strong, setting percentage is high in selection, the high numbered 10-5 individual plant of stigma exposing ratio is that male parent hybridizes with an aromatic plant metioned in ancient books 9311A, receives hybrid seed 30.
6th year positive season plantation BF1,30 seeds of Hainan results are planted 20 strains, are made recurrent parent sterile line similar to 10-5 to strain Leaf pattern with 10-5 and backcross.
6th year Dong-the seven year spring Hainan plantation BF2, continuation 10-5 make recurrent parent sterile line similar to 10-5 to strain Leaf pattern and backcross.
7th year positive season plantation BF3, makees recurrent parent sterile line similar to 10-5 to strain Leaf pattern with 10-5 and backcrosses.
7th year Dong-the eight year spring Hainan plantation BF4, continuation 10-5 make recurrent parent sterile line similar to 10-5 to strain Leaf pattern and backcross.
8th year positive season plantation BF5, makees recurrent parent sterile line similar to 10-5 to strain Leaf pattern with 10-5 and backcrosses.
BF6 is planted in 8th year Dong spring sowing in-the nine year, makees recurrent parent sterile line similar to 10-5 to strain Leaf pattern with 10-5 and backcrosses.
9th year positive season plantation BF7, makees recurrent parent sterile line similar to 10-5 to strain Leaf pattern with 10-5 and backcrosses.
BF8 is planted in 9th year Dong spring sowing in-the ten year, makees recurrent parent sterile line similar to 10-5 to strain Leaf pattern with 10-5 and backcrosses.Backcross sterile line character stable and consistent, essentially identical with 10-5 strain Leaf pattern.Sterile line is named: three leather 2A.Survey advantage is hybridized with multiple Indica Rice Restorer Lines and three leather 2A.
10th year positive season plantation Combination nova identifies advantage, and institute's combo is closed all can be the most solid, and hybrid vigor is strong.
The selection of a kind of novel japonica rice cytoplasmic male sterile line that the present invention provides, the novel Indonesia paddy field paddy type Cytoplasm japonica rice sterile line of selection-breeding, not only stable fertility, be there is no self-fertility phenomenon by Effect of Hyperthermic Environment, and combo can go out to produce the upper excellent Xian round-grained rice friendship Hybrid Rice Combination that can utilize.
The above; it is only the present invention preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; any those familiar with the art is in the technical scope that the invention discloses; according to technical scheme and inventive concept equivalent or change in addition thereof, all should contain within protection scope of the present invention.
Claims (3)
1. the selection of a novel japonica rice cytoplasmic male sterile line, it is characterized in that: the selection of this novel japonica rice cytoplasmic male sterile line is: utilize Indonesia's paddy field paddy type Cytoplasm indica cms line and japonica rice to keep system's hybridization, backcross through many generations, select Indonesia's paddy field paddy type Cytoplasm japonica rice sterile line；
Specifically comprise the following steps that
The excellent japonica rice of S1: selection-breeding Comprehensive Traits keeps system, japonica rice strain round-grained rice 9523(force fortune round-grained rice No. 7/round-grained rice 12-5 with yield is high, strain Leaf pattern is good) it is maternal, it is paternal hybrid with Japan's western light of japonica rice variety, with round-grained rice 9523/western light as female parent, doing paternal hybrid with blast resisting material round-grained rice 01-2, adding is commissioned to train educates；
S2: keep system hybridize and gather in the crops male-sterile seed with japonica rice with Indonesia paddy field paddy type Cytoplasm indica cms line (such as an aromatic plant metioned in ancient books 9311A, middle 9A, II-32A etc.), selecting the individual plant that plant type is good, meter Zhi Hao, disease resistance are strong, setting percentage is high, stigma exposing ratio is high in the F7 cultivated in S1 is that male parent hybridizes with an aromatic plant metioned in ancient books 9311A；
S3: keep system as recurrent parent and male-sterile seed continuous backcross using japonica rice, be returned to sterile line and keep being that character is basically identical with japonica rice, and stable neat；
S4: hybridize with indica restorer line with Indonesia's paddy field paddy type Cytoplasm japonica rice sterile of stable and consistent, selects excellent Xian round-grained rice and hands over Hybrid Rice Combination.
The selection of a kind of novel japonica rice cytoplasmic male sterile line the most according to claim 1, it is characterised in that: the rice cultivating place in described step S1 is Hainan and Anhui.
The selection of a kind of novel japonica rice cytoplasmic male sterile line the most according to claim 1, it is characterised in that: the cultivation algebraically that adding in described step S2 and S3 is commissioned to train educates is 6-10 generation.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220330A (en) * | 2017-12-30 | 2018-06-29 | 青岛袁策生物科技有限公司 | Utilize the method for Albino Seedling initiative rice sterile line |
| CN108849473A (en) * | 2018-06-28 | 2018-11-23 | 定远县鑫理想农业科技有限公司 | A kind of double season hybrid rice breeding methods |
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
| CN109717073A (en) * | 2019-03-12 | 2019-05-07 | 于春河 | A kind of novel fine cytoplasm sterile line breeding method |
| CN114009332A (en) * | 2021-07-14 | 2022-02-08 | 安徽红旗种业科技有限公司 | Multi-grain three-line japonica rice sterile line Tai765A breeding method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220330A (en) * | 2017-12-30 | 2018-06-29 | 青岛袁策生物科技有限公司 | Utilize the method for Albino Seedling initiative rice sterile line |
| CN108849473A (en) * | 2018-06-28 | 2018-11-23 | 定远县鑫理想农业科技有限公司 | A kind of double season hybrid rice breeding methods |
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
| CN109717073A (en) * | 2019-03-12 | 2019-05-07 | 于春河 | A kind of novel fine cytoplasm sterile line breeding method |
| CN114009332A (en) * | 2021-07-14 | 2022-02-08 | 安徽红旗种业科技有限公司 | Multi-grain three-line japonica rice sterile line Tai765A breeding method and application thereof |
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