CN114342798B - Breeding method of special rice for plant factory - Google Patents

Breeding method of special rice for plant factory Download PDF

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CN114342798B
CN114342798B CN202111615935.7A CN202111615935A CN114342798B CN 114342798 B CN114342798 B CN 114342798B CN 202111615935 A CN202111615935 A CN 202111615935A CN 114342798 B CN114342798 B CN 114342798B
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tillering
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CN114342798A (en
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吴殿星
诸方宁
王寅
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Zhejiang University ZJU
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Abstract

The invention discloses a method for breeding rice special for plant factoryThe method comprises hybridizing a multi-tillering rice plant as female parent and a plant type extremely compact rice plant as male parent to obtain F 1 Generation; and then with F 1 The generation is male parent, the mini small plant type rice is female parent, the filial generation is obtained by hybridization, the filial generation and the female parent are backcrossed, and the inbreeding is carried out to obtain BC 1 F 2 Seed generation; planting BC 1 F 2 Generation and plant line planting BC 1 F 3 Selecting single plants according to plant height, effective tillering and tillering angles; strain planting BC 1 F 4 Selecting and reserving a strain with strong sheath blight resistance, extremely compact plant type, short stalk and multiple tillers; strain planting BC 1 F 5 Carrying out density-tolerant cultivation, and selecting and reserving a high-yield high-quality disease-resistant new strain; BC 1 F 6 And (4) carrying out cultivation production tests in plant generation factories and finally selecting special industrial rice for plants. The rice obtained by the method has the remarkable characteristics of short and small plants, extremely compact plant types, multiple tillers, excellent rice quality and good disease resistance, and the iron-zinc micronutrient product has excellent quality and is particularly suitable for green, high-quality and intensive production.

Description

Breeding method of special rice for plant factory
Technical Field
The invention relates to the technical field of rice breeding, in particular to a method for breeding special rice for plant industrialization.
Background
The plant factory is a high-level stage of modern facility agricultural development, and is an agricultural production mode with high knowledge and technology density, which integrates and innovates multiple subjects such as modern biotechnology, building engineering, environmental control, mechanical transmission, material science, facility horticulture, computer science and the like. The plant factory carries out plant or crop cultivation production under the controllable facility environment, and has the remarkable advantage of annual high-efficiency production. The plant factory research and development and the industry in China show rapid development signs, and the domestic related output value reaches about 5 hundred million yuan RMB.
The rice is the staple food grain of China, and provides important guarantee for the grain safety. The rice production is combined with plant factories, the restriction of land, space and climate conditions on the traditional planting is solved, the multilayer three-dimensional cultivation can be realized, the production can be realized all the year round, and a new way for increasing the yield of grains is opened up.
Therefore, a breeding method suitable for plant factory special rice is needed to be provided, so that the bred rice is suitable for green high-quality intensive production and can also be used for experience type 'teaching through lively activities' farming culture.
Disclosure of Invention
The invention provides a method for breeding the special rice for plant factory production, the rice obtained by the breeding method has the remarkable characteristics of short and small plant, extremely compact plant type, multiple tillers, high rice quality and good disease resistance, is particularly suitable for green, high-quality and intensive production, and can also be used for experience type 'edutainment' farming culture.
The specific technical scheme is as follows:
a method for breeding the rice specially used for plant factory includes the following steps:
(1) Hybridizing the rice with multiple tillers as female parent and the rice with very compact plant type as male parent to obtain F 1 Generation; and then F 1 The generation is used as a male parent, the mini plantlet type rice is used as a female parent, hybridization is carried out, obtained filial generation is backcrossed with the mini plantlet type rice, and BC is obtained 1 F 1 Generation, BC 1 F 1 After generation selfing, harvesting BC 1 F 2 Seed generation;
(2) Mixing BC 1 F 2 The method comprises the following steps of (1) uniformly and thinly directly sowing and raising seedlings, selecting plants with the plant height of less than or equal to 25cm when seedlings are 5-6 leaves old, planting the plants individually, transplanting the plants into a field according to the row spacing of 20cm multiplied by 20cm, and selecting excellent individual plants with the plant height of less than or equal to 50cm, effective tillering of more than or equal to 40 per plant and tillering angle of less than or equal to 10 degrees when the seedlings are mature;
(3) To BC 1 F 3 Carrying out strain planting in generations, observing and identifying short stems, multi-tillering characteristics and extremely compact plant type stability, selecting and reserving excellent single plants with plant height of less than or equal to 50cm, effective tillering of more than or equal to 40 per plant and tillering angle of less than or equal to 10 degrees;
(4) To BC 1 F 4 Carrying out strain planting for the generation, identifying resistance to sheath blight indoors and outdoors, and selecting and reserving excellent strains with strong resistance to sheath blight, extremely compact plant types, short stems and multiple tillers;
(5) To BC 1 F 5 Carrying out strain planting on the generations, carrying out dense cultivation, identifying yield, quality and disease resistance, and selecting and reserving a high-yield high-quality disease-resistant new strain;
(6) To BC 1 F 6 And carrying out a plant factory cultivation production test, and finally selecting the plant factory special rice by taking the yield, the growth period and the plant height as comprehensive evaluation indexes.
Further, the multi-tillering type rice is Zhejiang rice 02, the plant type is extremely compact, zhemi rice No. 1, and the mini small-plant type rice is Zhejiang.
Zhejiang university mutagenized Yangdao rice No. 6 bred multi-tillering rice variety, has already been matched with two-line sterile line NHR111S to breed and examine a high-quality hybrid rice new variety Zhejiang rice double-excellent 12 (Zhe examined rice 2009012), and has already applied for the right protection and authorization of new plant varieties to agricultural rural departments (variety right book No. CNA 20080434.4).
Zhejiang university mutagenized Yangdao No. 6 rice variety with extremely compact plant type, zhejiang rice No. 1, has applied for plant new variety right protection and authorization to agricultural rural departments (variety title book No.: CNA 20120030.0).
The mini small plant type (mini type for short) rice variety bred by mutagenizing Yangdao No. 6 of Zhejiang university is slightly large in Zhejiang university, and the new plant variety right protection is applied to the agricultural rural department (application number: 20201007177).
Further, in the step (4), the method for identifying the resistance to sheath blight is referred to the literature (Hejia et al. Method for identifying resistance to rice blast and sheath blight. Plant science, 2020, 55 (5): 577-587).
Further, in the step (5), the judgment standard of high yield, high quality and disease resistance is that the yield is more than or equal to 550 kg/mu, the edible quality of rice reaches the second grade or more issued by the ministry, and rice blast resistance is achieved.
Further, in the step (6), the comprehensive evaluation index is: the yield is more than or equal to 700 kg/mu, the growth period is less than or equal to 130 days, and the plant height is less than or equal to 50cm.
Compared with the prior art, the invention has the following beneficial effects:
(1) The plant factory special rice bred by the invention has the remarkable characteristics of short and small plants, extremely compact plant types and multiple tillers, is particularly suitable for green, high-quality and intensive production, and meanwhile, the mini rice can also be used for experiential 'edutainment' farming culture.
(2) The plant factory special rice obtained by the invention selects three parents which are all derived from Yangdao No. 6, although the phenotypic characters are greatly different, the rice can be backcrossed for only 1 time under the same genetic background, the breeding period is obviously shortened (generally backcrossed for 4-5 times), and the rice has the outstanding advantages of short, flat, quick and high efficiency by adopting a breeding strategy.
(3) Aiming at the special scene requirements of industrial production, the invention designs and sets a breeding method of successive hybridization backcross according to the characteristics of parents in a targeted manner; mini-type rice Zhe is used as a backcross parent, and multiple tillers (obviously reducing the seed consumption) and extremely compact plant types (dense disease-resistant cultivation) are polymerized on the basis of short and small (convenient for spatial multi-layer utilization), so that the successful breeding probability is obviously improved, the practicability of the variety is greatly expanded, and the method has the outstanding advantages of higher efficiency and space utilization rate and contribution to yield increase.
(4) The rice bred by the method has high quality and good disease resistance, and particularly meets the strategic requirements of national green high-quality production. The iron and zinc micro-nutrient content is high, and the requirement of global public nutrition is met.
Detailed Description
The present invention will be further described with reference to the following specific examples, which are set forth merely to illustrate specific embodiments of the invention, but the scope of the invention is not limited thereto.
A breeding method suitable for plant factory special rice comprises the following specific steps:
(1) F obtained by hybridization of Zhejiang rice 02 as female parent and Zhejiang rice No. 1 as male parent 1 The filial generation is used as male parent, and is combined with Zhejiang as female parent, and then the Zhejiang as backcross parent is backcrossed for 1 time to obtain BC 1 F 1 Generation, BC 1 F 1 After generation selfing, BC is harvested 1 F 2 1200 seeds are used;
(2)BC 1 F 2 carrying out uniform and thin direct seeding for seedling raising, selecting 532 plants which are particularly short and have the plant height of less than or equal to 25cm when the seedlings are 5-6 leaves old, planting the plants in a single plant, transplanting the plants into a field according to the row spacing of 20cm multiplied by 20cm, and selecting 56 excellent single plants with the plant height of less than or equal to 50cm, effective tillering of more than or equal to 40 per plant and tillering angle of less than or equal to 10 degrees when the plants are mature;
(3)BC 1 F 3 planting in a strain, observing and identifying the stability of short stems, multiple tillering characteristics and extremely compact plant types, selecting and reserving 19 excellent lines with the plant height of less than or equal to 50cm, the effective tillering of more than or equal to 40 per plant and the tillering angle of less than or equal to 10 degrees;
(4)BC 1 F 4 the generation, strain planting, indoor and outdoor sheath blight resistance identification, and 11 excellent strains with strong sheath blight resistance, extremely compact plant types, short stems and multiple tillers are selected;
(5)BC 1 F 5 planting the strains, performing density-resistant cultivation identification, and selecting 5 high-yield and high-quality disease-resistant new strains, wherein the yield is more than or equal to 550 kg/mu, the rice eating quality reaches the second grade and above ministry of ministry and is resistant to rice blast;
(6)BC 1 F 6 and (3) generation, in-plant cultivation and production tests, selecting 1 rice suitable for plant factory special use and tentatively named 'multi-spike rice' by taking the short growth period and short plant height as the preferable comprehensive judgment indexes under the condition that the yield is more than or equal to 700 kg/mu.
The index character comparison of the variety in the sowing extraction period, the plant height, the effective tillering, the tillering angle, the sheath blight, the rice blast, the yield and the quality is shown in a table 1.
TABLE 1 comparison of index traits of slightly small Zhe size, zhe radiation 02 and Zhe Mi rice No. 1 selection for plant industrialized rice
Figure BDA0003436738880000031
The measurement method of each index in table 1 is as follows: the agronomic characters and the yield of the plants such as growth period, plant height and tillering are as follows: national rice variety test observation records items, methods and standards (trial); sheath blight: DB 34/T3306-2018; rice blast: DB 22-T2389-2018; polished rice rate and rice quality: NY/T593-2013; iron: GB/T5009.90-2003; zinc: GB/T5009.14-2003.
Through combined evaluation and identification, the bred special industrial rice with multiple ears has the advantages that the sowing and extraction period is 45 days, the plant height is 45cm, each plant has 52 effective tillers, the tillering angle is 0 degree, the rice sheath blight resistance and the rice blast resistance are realized, the field yield is 560 kg/mu, the industrial yield of the plant is 700 kg/mu, the whole polished rice rate is 60.6%, the rice quality reaches the ministry-issued first level, the iron content is 16.68mg/kg, the zinc content is 17.5mg/kg, and the micro-nutrition quality is excellent.
Compared with the parent Zhe-sized rice, zhe-radiance 02 and Zhe-Mi rice No. 1, the 'multi-spike rice' takes the advantages of the parent into consideration, has improved resistance to rice blast, is possibly related to uniform spike size and neat spike layer, has consistent maturity and higher rate of finished rice, and has rice quality reaching the ministry of ministry and first-level and exceeds the breeding expectation.
In addition, iron is an important component of hemoglobin and iron deficiency will lead to iron deficiency anemia. The zinc nutrition is a flower which is reputed to be micro-life, and is particularly important for the intelligence development of infants and children in vivo. The iron and zinc are the key points of microbial nutrition fortification, the contents of the two important nutrients of the multi-spike rice are 16.8mg/kg and 17.5mg/kg respectively,
therefore, the 'multi-spike rice' bred by the invention has high iron and zinc micronutrient content, which is needed by the current Chinese health and meets the global public nutrition requirement. The plant is shorter and smaller, is only 45cm, has extremely compact plant type, better rice quality and better disease resistance, and is more suitable for green high-quality intensive production.

Claims (3)

1. A method for breeding the rice specially used for plant factory is characterized by comprising the following steps:
(1) Taking multi-tillering type rice as a female parent and plant type extremely compact type rice as a male parent to carry out hybridization to obtain F 1 Generation; and then F 1 The generation is used as a male parent, the mini plantlet type rice is used as a female parent, hybridization is carried out, obtained filial generation is backcrossed with the mini plantlet type rice, and BC is obtained 1 F 1 Generation, BC 1 F 1 After generation selfing, harvesting BC 1 F 2 Seed generation; the multi-tillering type rice is Zhejiang rice 02, the plant type is extremely compact, zhejiang rice No. 1, and the mini small-plant type rice is Zhejiang rice;
(2) Mixing BC 1 F 2 The seedlings are uniformly and thinly sown directly for seedling cultivation, plants with the plant height of less than or equal to 25cm are selected when the seedlings are 5-6 leaves old, the plants are planted individually, the seedlings are transplanted to a field according to the row spacing of 20cm multiplied by 20cm, and excellent individual plants with the plant height of less than or equal to 50cm, the effective tillering of more than or equal to 40 per plant and the tillering angle of less than or equal to 10 degrees are selected when the seedlings are mature;
(3) To BC 1 F 3 Carrying out strain planting, observing and identifying the short stalk, the multi-tillering characteristic and the stability of extremely compact plant type, selecting and reserving plant height less than or equal to 50cm, and effectively tilleringMore than or equal to 40 excellent single plants per plant and with tillering angle less than or equal to 10 degrees;
(4) To BC 1 F 4 Carrying out strain planting for the generation, identifying resistance to sheath blight indoors and outdoors, and selecting and reserving excellent strains with strong resistance to sheath blight, extremely compact plant types, short stems and multiple tillers;
(5) To BC 1 F 5 Carrying out strain planting on the generations, carrying out dense cultivation, identifying yield, quality and disease resistance, and selecting and reserving a high-yield high-quality disease-resistant new strain;
(6) To BC 1 F 6 And finally, carrying out a plant factory cultivation production test, and finally selecting the plant factory special rice by taking the yield, the growth period and the plant height as comprehensive evaluation indexes.
2. A selective breeding method of plant factory dedicated rice as claimed in claim 1, wherein in step (5), said judgment standard of high yield, high quality and disease resistance is that the yield is not less than 550 kg/mu, the edible quality of rice reaches the second grade or more issued by Ministry, and rice blast resistance is achieved.
3. The method for selectively breeding the rice plant exclusively used in plant factory according to claim 1, wherein in the step (6), the comprehensive evaluation index is: the yield is more than or equal to 700 kg/mu, the growth period is less than or equal to 130 days, and the plant height is less than or equal to 50cm.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104273023A (en) * 2014-05-15 2015-01-14 浙江大学 Polymerization breeding method for rice
CN106489718A (en) * 2016-09-30 2017-03-15 杭州中泽生物科技有限公司 A kind of selection of edible high quality richness ferrum zinc Oryza sativa L.
WO2018032925A1 (en) * 2016-08-17 2018-02-22 湖南希望种业科技股份有限公司 Small-grain type sterile rice line breeding method, and simple seed production method for hybrid rice
CN108142277A (en) * 2018-02-12 2018-06-12 浙江大学 A kind of selection of the special rice variety in fish pond

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104273023A (en) * 2014-05-15 2015-01-14 浙江大学 Polymerization breeding method for rice
WO2018032925A1 (en) * 2016-08-17 2018-02-22 湖南希望种业科技股份有限公司 Small-grain type sterile rice line breeding method, and simple seed production method for hybrid rice
CN106489718A (en) * 2016-09-30 2017-03-15 杭州中泽生物科技有限公司 A kind of selection of edible high quality richness ferrum zinc Oryza sativa L.
CN108142277A (en) * 2018-02-12 2018-06-12 浙江大学 A kind of selection of the special rice variety in fish pond

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