CN109717073A - A kind of novel fine cytoplasm sterile line breeding method - Google Patents
A kind of novel fine cytoplasm sterile line breeding method Download PDFInfo
- Publication number
- CN109717073A CN109717073A CN201910183637.1A CN201910183637A CN109717073A CN 109717073 A CN109717073 A CN 109717073A CN 201910183637 A CN201910183637 A CN 201910183637A CN 109717073 A CN109717073 A CN 109717073A
- Authority
- CN
- China
- Prior art keywords
- sterile
- sterile line
- breeding
- rice
- gld09
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to breeding of hybridized rice fields, disclose a kind of novel fine cytoplasm sterile line breeding method, are hybrid rice japonica rice sterile line slewing backcross breeding.Sea 86-1 × GLD09-1 is selected to make parents, selection F1 generation sterile plant and winter current year take Hainan to be hybridized with H63 with the progeny material that 86-1 × GLD09-1 osculant carries abortion gene, and 6 circulations hybridization (backcrossing) from generation to generation is educated by Nan Fanbei and imports holding system, 86-1, GLD09-1 are gone to sea as the novel sterile cytoplasm sterile line H63A of genetic background to cultivate.The characteristics of novel sterile cytoplasm sterile line H63A of the invention resistance, wide adaptability, stigma exposing ratio is high, angle of glume opening is big, Hua Shizao, compatibility and coordinate force are strong, highly resistance blast resisting.The cenospecies combination prepared with H63A sterile line goes out very strong hybrid vigour in variable rate technology, and the development of China's breeding of hybridized rice cause is enriched new cytoplasm germ plasm resource by the breeding success of the novel sterile cytoplasm sterile line of H63A again.
Description
Technical field
The invention belongs to breeding of hybridized rice field, a kind of discovery of novel sterile cytoplasm and sterile line breeding technology neck
Domain more particularly to a kind of backcross breeding method of novel fine cytoplasmic sterile line.
Background technique
Two major classes are divided into regard to the widely applied Three-line Hybrid male sterility of rice cytoplasm in current China.
(1) sporinite male sterility is to be controlled such as Yebai, ridge type by the sporinite i.e. genotype of parent to belong to
Sporinite male sterility type, the Hybrid Rice that this kind of sterile cytoplasm sterile line is prepared are produced using very extensively.
(2) gametophyte male sterility is that the pollen abortion of sterile line occurred in the andro gamete stage, and the sterility of pollen is matched
The genotype of daughter itself determine as Yunnan type, BT type belong to gametophyte male sterility type, this kind of sterile cytoplasm infertility
Using than wide in the Japonica Hybrid combination production that system prepares.
(1) gametophyte male sterile cytoplasm Yunnan type, BT type are the commonly used types of China's Japonica Hybrid, and this type is not
Educate is to restore to select recovery face relatively narrow for C system restorer substantially.
(2) the Japonica Hybrid sterile line cytoplasm applied in production at present is more single, and breeding apolegamy receives objective limit
Also scarcity is difficult to make strong superior hybrid crosses combination again restorer processed.
(3) novel fine cytoplasm H63A sterile line is matched successfully, that is, enriches sterile line cytoplasm germ plasm resource, is also preparation
Strong superior hybrid crosses Combination nova opens new apolegamy approach out, has also widened the probability of sterile restoring system breeding combo.To be
The new development of China's Japonica Hybrid Rice Breeding cause accelerates paces process.
Summary of the invention
In view of the problems of the existing technology, it was found that a kind of slewing backcrossing of novel sterile cytoplasm, sterile line
Selection.
The invention is realized in this way expanding genetic distance background by artificial hybridization formulates new source of cytoplasm, it is a kind of
The discovery of novel sterile cytoplasm, selection slewing backcross transformation infertility system, method:
Further, the novel fine cytoplasm sterile line breeding method specifically includes:
The first step selects seawater rice varieties, sea 86-1 kind as mother's sheet, with (the Shen parent GLD09-1 independently cultivated
The imperial round-grained rice 38 of agriculture 265/) as male parent progress test cross；October 20 winter in the same year, Hainan, the farm Nan Bin, agriculture and animal husbandry team breeding base,
3 plants of male sterile plants are selected from F1 group, are kept being that 63B continues bagging test cross with the precocious japonica rice newly cultivated；
The test cross seed of second step, 100% infertility of harvest bagging selfing on January 24 continues to sow, and early April agriculture and animal husbandry team educates
The first backcross generation sterile plant rate 100% of kind base plantation, sterile rate reach 98%；Sterile plant is therefrom selected again, continues bagging with 63B
Backcrossing；The B2F1 of rice breeding base kind is returned sterile rate 100%, sterile 98% or more rate；
Third step, then the strain of 100% infertility is selected, bagging, which is carried out, with 63B continues to be returned；The B3F1 backcrossing in breeding base is not
Educate rate 100%, sterile rate 99%；The strain of 100% infertility is selected again, and bagging is carried out with 63B and continues to be returned；The B4F1 in breeding base
It is returned sterile rate 100%, sterile 99% or more rate；
4th step, then the strain of 100% infertility is selected, continue to be returned with 63B bagging；
5th step, winter current year Hainan, the farm Nan Bin, agriculture and animal husbandry team breeding base B6F1 be bred as with H63 and 86-1 ×
The abortion gene of GLD09-1 osculant material carrying simultaneously educates 6 backcross generations importing holding systems by Nan Fanbei, is gone to sea with cultivating
86-1, GLD09-1 are the novel sterile cytoplasm sterile line H63A sterile line of genetic background.
In conclusion advantages of the present invention good effect are as follows: the stigma exposing ratio of novel sterile cytoplasm sterile line H63A
The features such as height, angle of glume opening are big, Hua Shizao, coordinate force are high, wide adaptability, highly resistance blast resisting.It is prepared with H63A sterile line
Cenospecies combination, goes out very strong hybrid vigour in variable rate technology, the breeding of H63A sterile line is successfully by China's breeding of hybridized rice
The development of cause enriches new intension again.
Detailed description of the invention
Fig. 1 is the orientation backcross breeding method flow diagram of novel fine cytoplasmic sterile line provided in an embodiment of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Application principle of the invention is explained in detail with reference to the accompanying drawing.
As shown in Figure 1, novel fine cytoplasm sterile line breeding method provided in an embodiment of the present invention is rice japonica type infertility
The orientation back cross breeding selection of system:
In the rice breeding base of Jiamusi on July 28 in 2016, the seawater rice product of selection Guangzhou Zhanjiang Chen victory discovery
Kind, sea 86-1 kind carry out test cross as male parent with the parent GLD09-1 independently cultivated as mother's sheet.October winter in the same year
20,3 plants of male sterile plants were selected, with the morning newly cultivated in Hainan, the farm Nan Bin, agriculture and animal husbandry team breeding base from F1 group
Ripe japonica rice keeps being that 63B continues bagging test cross, and the test cross seed of 100% infertility of harvest bagging selfing on January 24 continues to sow, and 4
The first backcross generation sterile plant rate 100% of moon the first tenday period of a month agriculture and animal husbandry team's breeding base plantation, sterile rate reach 98% or so.It therefrom selects not again
Strain is educated, continues bagging backcrossing with 63B, the B2F1 of the rice breeding base kind of Kiamusze City in 2017 is returned sterile rate 100%, infertility
98% or more rate.The strain for selecting 100% infertility again, carries out bagging with 63B and continues to be returned.Hainan of in September, 2017, the agriculture of the farm Nan Bin
The B3F1 for herding group breeding base is returned sterile rate 100%, sterile rate 99% or so.Select again 100% infertility strain, with 63B into
Row bagging continues to be returned.In December, 2017 Hainan, the farm Nan Bin agriculture and animal husbandry team breeding base B4F1 be returned sterile rate 100%, no
Educate 99% or more rate.The strain for selecting 100% infertility again continues to be returned with 63B bagging.The rice breeding base of Jiamusi in 2018
Each strain character of B5F1 reaches unanimity, and plant shape is 63B essentially the same with holding, and sterile plant rate is still 100%, sterile rate
Still remain in 99% or more.Winter current year Hainan, the farm Nan Bin, agriculture and animal husbandry team breeding base B6F1 be bred as with H63 and 86-1
The abortion gene of × GLD09-1 osculant material carrying simultaneously educates 6 backcross generations importing holding systems by Nan Fanbei, to cultivate
Extra large 86-1, GLD09-1 are the novel sterile cytoplasm sterile line H63A sterile line of genetic background.
The abortion gene carried using osculant material keeps being to cultivate with extra large 86-1, GLD09- by Backcross introgression
1 is the neoplasm japonica rice H63A sterile line of genetic background.
As a result:
Economical character neat and consistent, stable fertility abortion is thorough, and so far a kind of novel fine cytoplasmic sterile line declaration is bred as.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (2)
1. a kind of novel fine cytoplasm sterile line breeding method, which is characterized in that the breeding of the novel fine cytoplasmic sterile line
Method provides hybrid rice japonica rice sterile line slewing backcross breeding method: using in 86-1, GLD09-1 filial generation of sea
Between profile material carry abortion gene, by slewing Backcross introgression keep be, cultivate with extra large 86-1, GLD09-1 for lose
Pass the neoplasm japonica rice H63A sterile line of background.
2. the slewing selection of novel fine cytoplasmic sterile line as described in claim 1, which is characterized in that described is new
The slewing selection of type cytoplasmic male sterile line specifically includes:
The first step, 2016 summer Jiamusi: selection makees female parent with sea 86-1 (seawater rice varieties), with free strain GLD09-1 (Shen Nong
265/ imperial round-grained rice 38) hybridized as male parent；October 20 winter current year, the farm Sanya, Hainan Nan Bin agriculture and animal husbandry team breeding base, F1
For in group's selection, selection finds 3 plants of male sterile plants,
2016 winter Sanya Nan Bin farms: second step keeps being H63 and 86-1, GLD09-1 filial generation sterile plant with precocious japonica rice
It is hybridized；Harvest F1 × H63 on January 24 hybridizes grain seed, and it is 0 that it is solid, which to be selfed bag, for observation, sterile degree 100%, continues to sow Shanxi
Generation,
Third step, 2016 spring Sanya Nan Bin farms: backcrossing F1 × H63, harvest B1F1 harvest hybridization grain seed, observation selfing bag knot
Actually 0, sterile degree 100% continues to sow Shanxi generation.
4th step, 2017 summer Jiamusi: backcrossing F1 × H63, B2F1 harvest hybridization grain seed, it is 0 that it is solid, which to be selfed bag, for observation, infertility
Degree 100% continues to sow Shanxi generation.
5th step, 2017 winter Sanya Nan Bin farms: backcrossing F1 × H63, B3F1 harvest hybridization grain seed, observation are selfed that bag is solid to be
0, sterile degree 100% continues to sow Shanxi generation.
6th step, 2017 spring Sanya Nan Bin farms: backcrossing F1 × H63, B4F1 harvest hybridization grain seed, observation are selfed that bag is solid to be
0, sterile degree 100% continues to sow Shanxi generation.
5th step, 2018 summer Jiamusi: backcrossing F1 × H63, B5F1 harvest hybridization grain seed, it is 0 that it is solid, which to be selfed bag, for observation, infertility
Degree 100% continues to sow Shanxi generation.
7th step, 2018 winter Sanya Nan Bin farms: backcrossing F1 × H63, B6F1 harvest hybridization grain seed, observation are selfed that bag is solid to be
0, sterile degree 100%, sterile plant rate 100%, fertility abortion is thorough.
Extra large 86-1, GLD09-1 are the novel sterile cytoplasm H63A sterile line economical character group neat and consistent of genetic background, complete
At the procedure of breeding.
Present invention utilizes the strong rice varieties of excellent resistance, the osculant for orienting quick backcross transformation carries abortion gene material
Material carries out breeding novel fine cytoplasmic sterile line, expands genetic distance background for Japonica Hybrid and provides new germ plasm resource.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910183637.1A CN109717073A (en) | 2019-03-12 | 2019-03-12 | A kind of novel fine cytoplasm sterile line breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910183637.1A CN109717073A (en) | 2019-03-12 | 2019-03-12 | A kind of novel fine cytoplasm sterile line breeding method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109717073A true CN109717073A (en) | 2019-05-07 |
Family
ID=66302208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910183637.1A Pending CN109717073A (en) | 2019-03-12 | 2019-03-12 | A kind of novel fine cytoplasm sterile line breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109717073A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686320A (en) * | 2015-04-01 | 2015-06-10 | 内江杂交水稻科技开发中心 | Cultivation method of aromatic high-quality rice sterile line |
| CN105918111A (en) * | 2016-05-12 | 2016-09-07 | 安徽理想种业有限公司 | Breeding method for novel japonica rice cytoplasmic sterile line |
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
-
2019
- 2019-03-12 CN CN201910183637.1A patent/CN109717073A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686320A (en) * | 2015-04-01 | 2015-06-10 | 内江杂交水稻科技开发中心 | Cultivation method of aromatic high-quality rice sterile line |
| CN105918111A (en) * | 2016-05-12 | 2016-09-07 | 安徽理想种业有限公司 | Breeding method for novel japonica rice cytoplasmic sterile line |
| CN108901827A (en) * | 2018-07-13 | 2018-11-30 | 重庆市农业科学院 | Japonica hybrid rice sterile line breeding method when a kind of early blossoming |
Non-Patent Citations (1)
| Title |
|---|
| 马文东: "软米水稻新品种龙粳38的选育及高产栽培技术", 《作物杂志》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108925423B (en) | A kind of heat-resisting germplasm selection of Rise's boot period | |
| CN105002191B (en) | A kind of Oryza sativa L. CYP704B2 gene mutation body and method for identifying molecules thereof and application | |
| CN106069718B (en) | The method of rape dihaploid induction system selection and breeding cabbage type rape cytoplasmic male sterile line | |
| CN103814814A (en) | Method for selective breeding of rice thermo-sensitive permanent genic male sterile line by adopting bridge parents | |
| CN102696474A (en) | Breeding and application of cytoplasmic male sterility restoring line of brassica napus rapeseed and radish | |
| Cao et al. | Chinese experiences in breeding three-line, two-line and super hybrid rice | |
| Diao et al. | Foxtail millet breeding in China | |
| CN105409762A (en) | Cyclic breeding method of cotton varieties | |
| CN104429919B (en) | A kind of method of cabbage type rape abnormity blade new restoring system initiative | |
| CN102475058B (en) | Seed selection method of cabbage type brassica juncea cytoplasmic male sterile line | |
| CN104429918B (en) | A kind of method of Wild cabbage type recessive epistatic interaction core infertility rape temporary maintainer line improvement | |
| CN102577933B (en) | Method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice and application of method | |
| CN108770678B (en) | Cross breeding and breeding method of sea rice and japonica rice restorer | |
| CN102726285B (en) | Preparation method, seed propagation method and two-line hybrid seed propagation method based on osms4 mutant | |
| Faruque et al. | Variations in green plant regeneration response from anthers of indica rice and their hybrids with japonica cv. Taipei 309 | |
| CN102524044A (en) | Method for breeding brassica napus and mustard cytoplasm male sterility restoring line | |
| CN109717073A (en) | A kind of novel fine cytoplasm sterile line breeding method | |
| CN105613257B (en) | A kind of samsara selection of cotton sterile line | |
| CN103947541A (en) | Breeding method of rice cytoplasmic male sterile line | |
| CN109691387B (en) | Breeding method of male sterile cabbage type rape hybrid | |
| CN107410015A (en) | A kind of selection of leafy mustard cytoplasmic male sterile line | |
| CN108935068B (en) | Method for improving fertility temperature sensitivity of brassica napus pol CMS sterile line | |
| CN112042529A (en) | Breeding method of tetraploid rice | |
| CN107484655B (en) | A kind of selection of anti-clubroot preserved sichuan pickle cytoplasm male sterility hybrid | |
| Nikova et al. | Nicotiana tabacum L. as a source of cytoplasmic male sterility in interspecific cross with N. alata Link & Otto |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190507 |
|
| RJ01 | Rejection of invention patent application after publication |