CN101358233A - QTL mapping range of bruchid resistance in mung bean - Google Patents
QTL mapping range of bruchid resistance in mung bean Download PDFInfo
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- CN101358233A CN101358233A CN 200710143831 CN200710143831A CN101358233A CN 101358233 A CN101358233 A CN 101358233A CN 200710143831 CN200710143831 CN 200710143831 CN 200710143831 A CN200710143831 A CN 200710143831A CN 101358233 A CN101358233 A CN 101358233A
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Abstract
The invention provides a linkage group mapping interval STSbr1-VrCS161of mungbean weevil resistance QTL, and the STSbr1 of the interval can be used as the molecular marker of selecting mungbean weevil resistance materials. The significance of the invention lies in providing a QTL mapping interval and providing a novel economic effective molecular breeding approach for auxiliary breeding of mungbean weevil resistance molecular markers and accelerating the breeding process to reduce the harm of the mungbean weevils.
Description
Technical field
The invention belongs to field of molecular breeding, the QTL mapping that relates to an anti-bean weevil of mung bean specifically is interval.
Background technology
Mung bean [Vigna radiate (L.) wiclzek] is the main edible legume crop of China.Yet, bean weevil (GenusCallosobruchus) is to mung bean and the most serious worldwide storage pest of other Vigna crops harm, mainly comprise Callosobruchus chinensis (Callosobrnchus.Chinesis L.), Callosobruchus maculatus (Callosobrnchus.maculates F.) and grey bean weevil (Callosobrnchus.phaseoli G.), wherein the heaviest with Callosobruchus chinensis and Callosobruchus maculatus harm.Bean weevil harm can cause the reduction of mung bean output, quality and percentage of germination.At mung bean in 1 life cycle, but because of 30%～56% of bean weevil harm lost units, heavier as infecting harm once more, even whole storehouse mung bean is impaired.Pest control with insecticide control bean weevil certainly will be caused the increase of mung bean production cost, also can pollution of ecological environment, threaten human body health.Therefore, the anti-bean weevil mung bean variety of seed selection is the optimal path of control bean weevil.
Since the eighties in 20th century, the edible beans genetic breeding scholar of states such as Japan, Thailand, Australia is devoted to the excavation and the utilization of anti-bean weevil gene.The anti-bean weevil mung bean genetic resources that filters out at present mainly contains Madagascar wild species TC1966, India cultivar V2709, Philippines cultivar V2802 and Australian wild species ACC41.TC1966 is the maximum anti-source of research, and Fujii etc. (1987) studies show that high anti-Callosobruchus chinensis of TC1966 and Callosobruchus maculatus.Kitamura etc. (1988) discover that TC1966 is controlled by the dominance single-gene the resistance of bean weevil, and (1998) such as Young etc. (1992) and Kaga have made up mung bean F respectively
2:3The genetic linkage maps of colony and Gao Dai backcross population, and all should be positioned on the 9th linkage group by anti-bean weevil major gene.(1996) such as Tomooka etc. (1992) and Watanasit utilize TC1966 to be the parent respectively and successfully cultivate the anti-bean weevil height of mung bean for strain.Ishimoto etc. (2004) are also attempting carrying out the separation of anti-bean weevil gene.Talekar etc. (1992) studies show that all anti-Callosobruchus chinensis of V2709 and V2802 and Callosobruchus maculatus, resistance main is imitated dominant gene control by 1, Prakit etc. (2007) studies show that V2709 and V2802 to the resistance of Callosobruchus chinensis and Callosobruchus maculatus probably in same site.
Quantitative character gene locus therefor QTLs (Quantitative trait loci) is meant that the variation to quantitative character has the individual gene or the closely linked gene cluster of considerable influence.The proposition of QTL is research mung bean quantitative character single gene action and make effect mutually, and the genetic analysis application of model of setting up on this basis provides theoretical foundation, for new way has been opened up in the practical enforcement of the anti-bean weevil breeding system of mung bean.
Kaga etc. (1996) utilize the middle mapping population of rice bean and red bean hybridization to locate 1 anti-Callosobruchus chinensis main effect QTL on the 2nd linkage group.Somta etc. (2006) utilize cultivation rice bean [Vigna.umbellate (Thunb.) Ohwi﹠amp; Ohashi] and wild red bean [Vigna.nakashimae (Ohwi) Ohwi ﹠amp; Ohashi] hybridization F
2For mapping population, Callosobruchus chinensis and Callosobruchus maculatus to different development stage carry out dynamic Q TL detection and hereditary effect analysis, on the 7th linkage group, detect 1 stable anti-Callosobruchus chinensis main effect QTL, on the 3rd linkage group, detect 1 stable anti-Callosobruchus maculatus main effect QTL.At present, yet there are no the report of the anti-bean weevil qtl analysis of relevant mung bean.
Summary of the invention
The object of the present invention is to provide the linkage group of an anti-bean weevil QTL of the mung bean as shown in Figure 1 interval STSbr1～VrCS161 that maps, this interval STSbr1 can be used as the molecule marker of selecting the anti-bean weevil material of mung bean.
The present invention utilizes the F of 227 strain systems that hybridization obtains in Australian high sense bean weevil mung bean cultivar Berken (Vigna.radiata ssp.radiata) and high anti-bean weevil mung bean wild species ACC41 (Vigna.radiata ssp.sublobata) subspecies
9-10Reach the genetic linkage maps that comprises 79 RFLP molecule markers that makes up in view of the above for recombinant inbred strain (Recombinant inbred line) colony, by (promptly 2000 in Australia under 3 planting environment conditions to continuous 2 years (promptly in July, 2005 and in July, 2006), 2005 in Beijing, Nanjing) indoors artificial of RIL colony of results connects worm and identifies, estimates the anti-Callosobruchus chinensis performance of recombinant inbred strain colony respectively.
Utilize the composite interval mapping method of Windows QTL cartographer Version 1.13 mapping softwares, as the examination criteria that QTL occurs, the seed rate of being injured of recombinant inbred strain is carried out the QTL mapping with LOD value 〉=3.0.The result has found to repeat under four time * place and environments interval STSbr1～VrCS161 of QTL in the I linkage group, as shown in Figure 1.
According to the naming method of Humphry (2005), will be positioned at this QTL called after brI of I linkage group to QTL.This anti-Callosobruchus chinensis QTL, the LOD value 12.99～82.74,74.05%～79.27% of interpret table form variation is 4.0～8.0cM apart from the distance of the mark STSbr1 of left wing.The additive effect of this QTL is a negative value, shows that this site allelotrope from maternal Berken can increase seed 40.75%～45.18% of the rate of being injured, and promptly resistance allele is from male parent ACC41 (table 1).Therefore, no matter from the distance of anti-Callosobruchus chinensis QTL and STSbr1 or from the contribution rate of QTL to phenotype, STSbr1～VrCS161 is the ideal mark interval of anti-Callosobruchus chinensis.Utilize the significance of the interval STSbr1～VrCS161 of mapping of an anti-Callosobruchus chinensis QTL of mung bean as shown in Figure 1 provided by the invention to be, be the anti-bean weevil molecular mark of mung bean, accelerate breeding process, improving breeding efficiency provides a kind of cost-effective molecular breeding new way.
The QTL mapping of four in table 1 time * anti-Callosobruchus chinensis of place mung bean
Description of drawings
Fig. 1: the mapping interval of anti-Callosobruchus chinensis QTL on linkage group I.
This figure is by mung bean recombinant inbred strain colony (the molecule marker linkage map of the linkage group I that Berken * ACC41) makes up.Hollow rectangle line among the figure is represented linkage group, and the left side numeral is the genetic distance between the mark, and unit is cM, and the right side is the title of RFLP molecule marker, and * * expressive notation shows separating extremely significantly partially of 0.05 level.Wherein Vr_ represents the mung bean clone, Lp_ Biao Shi Bian beans clone, and STSbr_ represents mung bean STS mark.
The trilateral on linkage group right side represents that the QTL mapping under the different year varying environment is interval, and wherein white is represented 2005 Australia, light gray expression 2006 Australia, and grey colour specification 2006 Beijing, black is represented 2006 Nanjing.The QTL mapping interval in four time * places is all at STSbr1～VrCS161.
Embodiment
Embodiment: the indoors artificial of anti-Callosobruchus chinensis connects worm and identifies
Be to preserve germplasm, seed quality and group structure still be under the situation of random population in guaranteeing family, and choosing respectively that 2 of gathering in the crops in Australian land for growing field crops in 2000 repeat respectively is 210 and 217 s' F
9RIL is a test materials, and the indoors artificial that adopts national science and technology research project to select for use connects the worm method, respectively at connecing worm in July, 2005 and in July, 2006.And in 2006 with 2005 respectively in Beijing and Nanjing plant 72 and 59 F10RIL colonies of 210 parts of F9RIL of one of them multiple results and be used to connect worm.Connect worm in 2 years and all compare for No. 1, establish 2 repetitions with medium green.60 healthy seeds of picked at random are divided into 2 parts from each family, and every part of 30 seeds are put into the small plastic box of diameter 6cm and high 1cm respectively, do not add a cover, and (66cm * 44cm * 18cm), 2 layers of black cloth of upper cover place and support on the worm frame to put into big plastics casing.Support the worm temperature and be controlled at (27 ± 2) ℃, and keep dark and relative high humidity.Put into the adults of 400～500 couples of 1～3d that just sprouted wings in each big plastics casing, be allowed to condition in the box and lay eggs at random on each material, the ovum amount and remove adult when above about 5 greatly, will connect the worm material and continue to cultivate in the insectary to feeling every seed of worm parent.Wait to contrast No. 1 seed rate of being injured of medium green and reach at 100% o'clock, investigate a number of being injured of every part of material, be converted into seed percentage of injury (percentage ratio of promptly being injured).
The linkage group of the anti-Callosobruchus chinensis QTL of mung bean the determining of interval STSbr1～VrCS161 of mapping
Utilize the structure of the genetic linkage maps with 79 RFLP molecule markers of this RIL colony structure to see document (Humphry etc., 2005).Molecule marker in the collection of illustrative plates is distributed on 13 linkage groups uniformly, has covered the about 684.7cM of mung bean genome, the shortest linkage group 5.6cM wherein, and the longest linkage group 85.9cM, mean distance is 10.4cM between mark, ultimate range is 20.7cM.
Composite interval mapping method (composite intervalmapping) with Windows QTL cartographer Version 1.13 mapping softwares, as the examination criteria that QTL occurs, the seed rate of being injured of recombinant inbred strain is carried out the QTL mapping with LOD value 〉=3.0.The result has found to repeat under the 4 years * place and environment interval STSbr1～VrCS161 of QTL in the I linkage group, shown in Fig. 1, the table 1.
Anti-Callosobruchus chinensis QTL 74.05%～79.27%, is 4.0～8.0cM apart from the distance of the mark STSbr1 of left wing to the contribution rate of phenotypic variation in STSbr1～VrCS161 interval.Therefore, no matter from the distance of anti-Callosobruchus chinensis QTL and STSbr1 or from the contribution rate of QTL to phenotype, STSbr1～VrCS161 is the ideal mark interval of anti-Callosobruchus chinensis.The additive effect of anti-Callosobruchus chinensis QTL is a negative value in STSbr1～VrCS161 interval, shows to be that resistance allele is from male parent ACC41.Utilizing STSbr1 to carry out molecule marker when selecting anti-bean weevil mung bean material, reject the breeding material that has same tag with Berken.
Claims (3)
- Interval STSbr1～the VrCS161 of the mapping of the linkage group I of 1 one anti-Callosobruchus chinensis QTL of mung bean.
- 2 intervals according to claim 1, its STSbr1 is as the molecule marker of selecting the anti-bean weevil material of mung bean.
- 3 each application in the mung bean molecular breeding according to claim 1 and 2.
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| CN 200710143831 CN101358233A (en) | 2007-08-03 | 2007-08-03 | QTL mapping range of bruchid resistance in mung bean |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103109735A (en) * | 2013-02-28 | 2013-05-22 | 江苏省农业科学院 | Method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans |
| CN105087768A (en) * | 2014-10-22 | 2015-11-25 | 中国农业科学院作物科学研究所 | Molecular marker assisted method for selectively breeding bruchid-resistant mung bean varieties |
| CN105368956A (en) * | 2015-12-11 | 2016-03-02 | 河北省农林科学院粮油作物研究所 | Molecular marker for assisted selection of green bean bruchid-resistant new gene Br3 and application of molecular marker |
| CN114381545A (en) * | 2022-01-21 | 2022-04-22 | 河北省农林科学院粮油作物研究所 | Molecular marker closely linked with bean weevil resistance QTL (quantitative trait locus) and application thereof in bean weevil resistance breeding |
-
2007
- 2007-08-03 CN CN 200710143831 patent/CN101358233A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103109735A (en) * | 2013-02-28 | 2013-05-22 | 江苏省农业科学院 | Method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans |
| CN103109735B (en) * | 2013-02-28 | 2015-04-08 | 江苏省农业科学院 | Method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans |
| CN105087768A (en) * | 2014-10-22 | 2015-11-25 | 中国农业科学院作物科学研究所 | Molecular marker assisted method for selectively breeding bruchid-resistant mung bean varieties |
| CN105087768B (en) * | 2014-10-22 | 2017-12-08 | 中国农业科学院作物科学研究所 | A kind of method of the anti-bean weevil kind of molecular marking supplementary breeding mung bean |
| CN105368956A (en) * | 2015-12-11 | 2016-03-02 | 河北省农林科学院粮油作物研究所 | Molecular marker for assisted selection of green bean bruchid-resistant new gene Br3 and application of molecular marker |
| CN105368956B (en) * | 2015-12-11 | 2019-01-08 | 河北省农林科学院粮油作物研究所 | A kind of molecular labeling and its application for the anti-bean weevil gene Br3 assisted Selection of mung bean |
| CN114381545A (en) * | 2022-01-21 | 2022-04-22 | 河北省农林科学院粮油作物研究所 | Molecular marker closely linked with bean weevil resistance QTL (quantitative trait locus) and application thereof in bean weevil resistance breeding |
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Open date: 20090204 |