CN103109735B - Method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans - Google Patents
Method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans Download PDFInfo
- Publication number
- CN103109735B CN103109735B CN201310062684.3A CN201310062684A CN103109735B CN 103109735 B CN103109735 B CN 103109735B CN 201310062684 A CN201310062684 A CN 201310062684A CN 103109735 B CN103109735 B CN 103109735B
- Authority
- CN
- China
- Prior art keywords
- bean
- weevil
- bruchid
- semi
- bean weevil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000011453 Vigna umbellata Nutrition 0.000 title claims abstract description 143
- 240000001417 Vigna umbellata Species 0.000 title claims abstract description 142
- 238000000034 method Methods 0.000 title claims abstract description 33
- 238000009396 hybridization Methods 0.000 title claims abstract description 27
- 235000010716 Vigna mungo Nutrition 0.000 claims abstract description 18
- 241000196324 Embryophyta Species 0.000 claims abstract description 17
- 238000012216 screening Methods 0.000 claims abstract description 14
- 238000009395 breeding Methods 0.000 claims abstract description 9
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 241000254171 Curculionidae Species 0.000 claims description 109
- 241001143309 Acanthoscelides obtectus Species 0.000 claims description 22
- 230000006378 damage Effects 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 18
- 238000011156 evaluation Methods 0.000 claims description 12
- 239000004033 plastic Substances 0.000 claims description 12
- 238000012797 qualification Methods 0.000 claims description 12
- 230000006698 induction Effects 0.000 claims description 6
- 102000002322 Egg Proteins Human genes 0.000 claims description 5
- 108010000912 Egg Proteins Proteins 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 claims description 5
- 210000004681 ovum Anatomy 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 4
- 238000003860 storage Methods 0.000 abstract description 7
- 239000000447 pesticide residue Substances 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 241000219977 Vigna Species 0.000 description 10
- 241001313742 Callosobruchus chinensis Species 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000000575 pesticide Substances 0.000 description 6
- 235000013305 food Nutrition 0.000 description 4
- IHGSAQHSAGRWNI-UHFFFAOYSA-N 1-(4-bromophenyl)-2,2,2-trifluoroethanone Chemical compound FC(F)(F)C(=O)C1=CC=C(Br)C=C1 IHGSAQHSAGRWNI-UHFFFAOYSA-N 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- 239000003905 agrochemical Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 238000003958 fumigation Methods 0.000 description 3
- 235000021374 legumes Nutrition 0.000 description 3
- 101000654381 Homo sapiens Sodium channel protein type 8 subunit alpha Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 102100031371 Sodium channel protein type 8 subunit alpha Human genes 0.000 description 2
- 240000007098 Vigna angularis Species 0.000 description 2
- 235000010711 Vigna angularis Nutrition 0.000 description 2
- 240000004922 Vigna radiata Species 0.000 description 2
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 2
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 2
- DFWFIQKMSFGDCQ-UHFFFAOYSA-N deethylatrazine Chemical compound CC(C)NC1=NC(N)=NC(Cl)=N1 DFWFIQKMSFGDCQ-UHFFFAOYSA-N 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 206010003694 Atrophy Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 108010016634 Seed Storage Proteins Proteins 0.000 description 1
- 241000003433 Vigna minima Species 0.000 description 1
- 241000039850 Vigna nakashimae Species 0.000 description 1
- 241000040274 Vigna riukiuensis Species 0.000 description 1
- 235000010726 Vigna sinensis Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 230000016571 aggressive behavior Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of plant genetics and breeding, and provides a method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans. The method comprises the steps of: taking bruchid-resistant rice beans as male parents and semi-natural red beans as female parents; hybridizing to obtain F1 generation of seeds, screening out a new variety of the semi-natural bruchid-resistant red beans by bruchid-resistant identification; hybridizing the new variety of the semi-natural bruchid-resistant red beans with the common red bean variety; carrying out bruchid-resistant identification selection on hybridized offspring seeds, screening to obtain a single plant with resistance; taking a cultivated variety as a backcross female parents and bruchid-resistant hybridized generation as the male parents to carry out rotation cross; identifying the backcross offspring, so as to obtain the variety of the red beans with the bruchid-resistant characteristic. By adopting the method for obtaining the bruchid-resistant red beans by the hybridization of the semi-natural bruchid-resistant red beans, the cost is saved; the pesticide residue is reduced; the body health of an eater is ensured; the storage period of the red beans is prolonged; the commercial quality of the red beans is improved; and the status of the red bean commodity in competition at home and abroad is also powerfully improved.
Description
Technical field
The invention belongs to Crop Genetic Breeding technical field, particularly relate to the method that the hybridization of a kind of semi-wild anti-bean weevil red bean obtains anti-bean weevil red bean.
Background technology
Red bean originates from China, and in the southeast, Asia, there is long cultivation history in northeast and Himalaya highland country, one of main Food Legume of Ye Shi China, and its protein content is high, and containing several amino acids, vitamin and abundant calcium, iron, P elements.Although red bean is regarded as " little crop ", in China, long-term sowing area has reached 400,000 hectares, Zong produce 680,000 tons, in the food consumption composition and international Food Legume trade of Asian ancestry crowd, occupies critical role.China's Germplasm Resource of Vigna Anglaris Ohwi Ohashi is very abundant, and cut-off Eleventh Five-Year Plan end, existing more than 8000 part of Germplasm Resource of Vigna Anglaris Ohwi Ohashi is catalogued, put in storage, accounts for 56% of world's Germplasm Resource of Vigna Anglaris Ohwi Ohashi.
Boring pest adzuki bean weevil seriously annoyings production and the storage of Chinese red bean always, also governs the foreign exchange earning of Chinese red bean.China's red bean Annual planting area has been increased to more than 6,000,000 mu, and wherein 10-15% is used for outlet, due to not anti-bean weevil, about has about 50% red bean quality to incur loss every year.Every year because bean weevil harm is with regard to lost units 1.7-2.6 hundred million kilograms, cause direct economic loss 8.8-10.4 hundred million yuan.
Adzuki bean weevil is the important stored grain insects of the Food Legumes such as red bean, mung bean, cowpea, in office which kind of to plant and the place of preserving red bean all can occur.In China, adzuki bean weevil is generally annual there are for 5 ~ 8 generations, and south can reach for 12 generations.The First aggression of adzuki bean weevil occurs in field, and adult lays eggs on young pod, takes food after ovum sprouts wings into larva through pod moth people Interior Seed, and is with in people storehouse with red bean results and endangers, and causes secondary infection after adult eclosion.Within a life cycle, because the harm of adzuki bean weevil can lost units 30 ~ 56%, as heavier in again infected harm, even whole storehouse red bean is damaged.
At present, the method for production preventing and treating bean weevil harm is mainly aluminum phosphide fumigation method, this not only adds edible beans production cost, and easily causes residue of pesticide, cause environmental pollution, affect eater healthy.The harm of control bean weevil is the most economical and the method for environmental protection cultivates anti-bean weevil kind, utilizes the resistance of crop itself to prevent bean weevil from endangering.
Red bean is the main host plants of adzuki bean weevil, in order to find anti-bean weevil Germplasm Resource of Vigna Anglaris Ohwi Ohashi, multiple domestic and international research institution has carried out anti-bean weevil to the cultivation of collecting and wild Germplasm Resource of Vigna Anglaris Ohwi Ohashi and has evaluated, but up to now, in existing Germplasm Resource of Vigna Anglaris Ohwi Ohashi, do not find anti-bean weevil germplasm materials, most of Germplasm Resource of Vigna Anglaris Ohwi Ohashi shows as feels bean weevil completely.But, in several Wild related germplasm of red bean, found the material that can resist bean weevil.
Owing to lacking direct anti-bean weevil Germplasm Resource of Vigna Anglaris Ohwi Ohashi, both at home and abroad about the report of red bean anti-bean weevil breeding is less.The anti-bean weevil breeding of red bean is based upon on the basis of other species of Vigna, and cultivation rice bean is considered to important anti-bean weevil gene source, because it has complete resistance to 3 kinds of bean weevils, and its edible safety is proved to be.The approach of what the anti-bean weevil breeding of red bean adopted at present is embryo rescue and Bridge parent.But the cross compatibility between red bean and rice bean is very low, when with red bean as female parent time, the tender hybridization pod of children generally can normal development, but growth about 10d, and it is sterile that young pod starts atrophy; When then can not normally bear pods as during female parent with rice bean.Before the non-abortion of hybridization pod, carry out IMMATURE EMBRYOS CULTURE, F1 generation plant can be obtained also normally solid.Kaga etc. utilize red bean " Erimoshouzu " to make female parent and rice bean " Kagoshima " is hybridized, and obtain Progeny plants by embryo rescue.Siriwardhane etc. confirm that red bean Wild related germplasm V. riukiuensis can be used as the Bridge parent between red bean and rice bean.Tomooka etc. confirmed again that red bean Wild related germplasm V.nakashimae, V.minima also can be used as the Bridge parent between red bean and rice bean afterwards, thus expanded the selectable scope of Bridge parent further.
Because anti-bean weevil characteristic comes from common rice bean, relevant genetically modified biosafety debate itself can not be caused.Bean weevil kind mass-energy as anti-in this is applied to production, will the storage period of China's red bean greatly be lengthened, is on a good wicket during China's red bean is at home and abroad competed.
Summary of the invention
The invention provides the method that the hybridization of a kind of semi-wild anti-bean weevil red bean obtains anti-bean weevil red bean, the method being intended to solve control bean weevil harm is at present mainly aluminum phosphide fumigation method, not only increase edible beans production cost, and easily cause residue of pesticide, cause environmental pollution, affect the problem that eater is healthy.
The object of the present invention is to provide a kind of semi-wild anti-bean weevil red bean to hybridize the method obtaining anti-bean weevil red bean, the method comprises the following steps:
Step one, makes male parent with anti-bean weevil rice bean, semi-wild red bean makes female parent, and hybridization obtains F1 generation seed, and goes out anti-bean weevil semi-wild red bean new germ plasm by anti-bean weevil evaluation and screening;
Step 2, hybridizes anti-bean weevil semi-wild red bean new germ plasm and conventional red bean kind, carries out anti-bean weevil qualification selection respectively, the individual plant with resistance that screening obtains to offspring's seed that hybridization obtains;
Step 3, backcrosses for male parent using cultivar as the female parent that backcrosses, with anti-bean weevil filial generation, after 5 generations that backcrossed, differentiates backcross progeny, obtains the red bean kind with anti-bean weevil characteristic.
Further, in step 2, conventional red bean kind is No. 1, Yangtze river basin main breed Su Hong.
Further, anti-bean weevil qualification adopts indoors artificial to connect worm method, by each breeding lines and anti-, the healthy seed of sense parent puts into the small plastic box of Φ 6cm and H1cm respectively, do not add a cover, put into large plastic casing at random, upper cover two layers of black cloth, be placed on foster worm frame, insectary temperature controls at 27 DEG C ± 2 DEG C, and keep dark and relative high humidity, 400 ~ 500 adults to the 1 ~ 3d that just sprouts wings are put in each large plastic casing, on average every part of identified material 8 is right, in box, each material is laid eggs at random, to sense worm parent every seed ovum amount more than 5 time, take out connecing worm material, remove all adults, after worm 40 ~ 45d waiting, investigate a number of being injured for every part of material, and evaluate resistance class according to rate of being injured.
Further, according to the evaluation criterion of rate evaluation resistance class of being injured be:
When seed rate of damage is 0 ~ 10%, be then high resistance bean weevil kind, grade is 1 grade;
When seed rate of damage is 10.1 ~ 35%, be then anti-bean weevil kind, grade is 3 grades;
When seed rate of damage is 35.1 ~ 65%, then anti-bean weevil kind in being, grade is 5 grades;
When seed rate of damage is 65.1 ~ 90%, be then induction reactance bean weevil kind, grade is 7 grades;
When seed rate of damage is greater than 90%, be then high induction reactance bean weevil kind, grade is 9 grades.
Further, in step 3, using cultivar as the female parent that backcrosses, with anti-bean weevil filial generation for male parent backcross time, need to add generation in Hainan and greenhouse.
The hybridization of semi-wild provided by the invention anti-bean weevil red bean obtains the method for anti-bean weevil red bean, makes male parent, semi-wild red bean makes female parent with anti-bean weevil rice bean, and hybridization obtains F1 generation seed, and goes out anti-bean weevil semi-wild red bean new germ plasm by anti-bean weevil evaluation and screening; Anti-bean weevil semi-wild red bean new germ plasm and conventional red bean kind are hybridized, anti-bean weevil qualification selection is carried out respectively, the individual plant with resistance that screening obtains to offspring's seed that hybridization obtains; Backcross for male parent using cultivar as the female parent that backcrosses, with anti-bean weevil filial generation, after 5 generations that backcrossed, differentiate backcross progeny, obtain the red bean kind with anti-bean weevil characteristic; The hybridization of this semi-wild anti-bean weevil red bean obtains the method for anti-bean weevil red bean, cost-saving, improve economic benefit, reduce residue of pesticide, ensure that eater's is healthy, extend red bean storage period, improve the marketing quality of red bean, effectively improve red bean commodity at home and abroad compete in status, there is stronger propagation and employment and be worth.
Accompanying drawing explanation
Fig. 1 is the realization flow figure of the method for the anti-bean weevil red bean of semi-wild anti-bean weevil red bean hybridization acquisition that the embodiment of the present invention provides;
Fig. 2 is the crossover process schematic diagram of the anti-bean weevil red bean of semi-wild anti-bean weevil red bean hybridization acquisition that the embodiment of the present invention provides.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is described in further detail.Should be appreciated that specific embodiment described herein only in order to explain the present invention, and be not used in restriction invention.
The semi-wild anti-bean weevil red bean hybridization that Fig. 1 shows the embodiment of the present invention to be provided obtains the realization flow of the method for anti-bean weevil red bean.
The method comprises the following steps:
In step S101, make male parent, semi-wild red bean makes female parent with anti-bean weevil rice bean, hybridization obtains F1 generation seed, and goes out anti-bean weevil semi-wild red bean new germ plasm by anti-bean weevil evaluation and screening;
In step s 102, anti-bean weevil semi-wild red bean new germ plasm and conventional red bean kind are hybridized, anti-bean weevil qualification selection is carried out respectively, the individual plant with resistance that screening obtains to offspring's seed that hybridization obtains;
In step s 103, backcross for male parent using cultivar as the female parent that backcrosses, with anti-bean weevil filial generation, after 5 generations that backcrossed, differentiate backcross progeny, obtain the red bean kind with anti-bean weevil characteristic.
In embodiments of the present invention, in step s 102, conventional red bean kind is No. 1, Yangtze river basin main breed Su Hong.
In embodiments of the present invention, anti-bean weevil qualification adopts indoors artificial to connect worm method, by each breeding lines and anti-, the healthy seed of sense parent puts into the small plastic box of Φ 6cm and H1cm respectively, do not add a cover, put into large plastic casing at random, upper cover two layers of black cloth, be placed on foster worm frame, insectary temperature controls at 27 DEG C ± 2 DEG C, and keep dark and relative high humidity, 400 ~ 500 adults to the 1 ~ 3d that just sprouts wings are put in each large plastic casing, on average every part of identified material 8 is right, in box, each material is laid eggs at random, to sense worm parent every seed ovum amount more than 5 time, take out connecing worm material, remove all adults, after worm 40 ~ 45d waiting, investigate a number of being injured for every part of material, and evaluate resistance class according to rate of being injured.
In embodiments of the present invention, according to the evaluation criterion of rate evaluation resistance class of being injured be:
When seed rate of damage is 0 ~ 10%, be then high resistance bean weevil kind, grade is 1 grade;
When seed rate of damage is 10.1 ~ 35%, be then anti-bean weevil kind, grade is 3 grades;
When seed rate of damage is 35.1 ~ 65%, then anti-bean weevil kind in being, grade is 5 grades;
When seed rate of damage is 65.1 ~ 90%, be then induction reactance bean weevil kind, grade is 7 grades;
When seed rate of damage is greater than 90%, be then high induction reactance bean weevil kind, grade is 9 grades.
In embodiments of the present invention, in step s 103, using cultivar as the female parent that backcrosses, with anti-bean weevil filial generation for male parent backcross time, need to add generation in Hainan and greenhouse.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As shown in Figures 1 and 2, technical scheme of the present invention is:
The creation of A semi-wild anti-bean weevil red bean new germ plasm
Utilize anti-bean weevil rice bean and semi-wild red bean preparing hybrid to combine, attention semi-wild red bean does female parent, and anti-bean weevil rice bean is male parent (reciprocal cross success rate only has orthogonal 10%).Obtain first familiar generation seed.The filial generation seed of results is carried out adding generation in Hainan winter, carries out the Preliminary Identification of anti-bean weevil in field, above filial generation is carried out to the qualification again of anti-bean weevil simultaneously in laboratory.It is for subsequent use that the individual plant filtering out high resistance bean weevil by comprehensive selection method is left to lower season.
B semi-wild anti-bean weevil red bean new germ plasm is hybridized with cultivation red bean
The anti-bean weevil semi-wild red bean new germ plasm that a upper season is obtained and Yangtze river basin main breed Su Hong No. 1 preparing hybrid combine.Anti-bean weevil selection being carried out respectively to offspring's individual plant of the hybrid combination obtained, staying standby rear use to screening the individual plant with above resistance obtained.
C obtains the red bean new germ plasm with anti-bean weevil characteristic by the method progressively backcrossed with conventional parent
By using cultivar as the female parent that backcrosses, backcross for male parent with anti-bean weevil filial generation, in 5 generations that backcrossed, to BC5 (adding generation in Hainan and greenhouse), are differentiated backcross progeny, make it have anti-bean weevil characteristic.
Beneficial effect: 1, cost-saving, increase economic efficiency: the anti-bean weevil red bean new germ plasm of acquisition is compared with traditional not anti-bean weevil red bean, thoroughly can prevent the aluminum phosphide fumigation during the agricultural chemicals of adzuki bean weevil and seed storage in abandoning tradition field, cost-saving 40-60 unit/mu, reduces the production cost of red bean.Simultaneously under the prerequisite not affecting quality, the harm that bean weevil produces red bean can be reduced, improve output and reach more than 20%;
2, reducing residue of pesticide, there is not genetically modified safety issue, environmental friendliness in environmental friendliness: traditional medicine insect protected easily causes residue of pesticide, causes environmental pollution, affects eater healthy, and the new germ plasm non agricultural chemical residuum utilizing the method to obtain yet;
3, extend red bean storage period, improve the marketing quality of red bean, improve effectively red bean commodity at home and abroad compete in status.
The present invention utilizes semi-wild red bean for medium, with anti-bean weevil rice bean
hybridize with semi-wild red bean (♀), hybridize with conventional red bean again after obtaining first familiar generation seed afterwards and this parent repeatedly backcrosses, successfully obtain the cultivation red bean new germ plasm of anti-bean weevil.Prove that semi-wild red bean can be used as the Bridge parent between red bean and rice bean, expand the selectable scope of Bridge parent.
The enforcement time: 2004-2010
Implement place: academy of agricultural sciences of Jiangsu Province, the numerous base of South of Hainan, academy of agricultural sciences of Jiangsu Province
Embodiment:
The creation of A semi-wild anti-bean weevil red bean new germ plasm
Utilize anti-bean weevil rice bean (Tr001, introduce from agriculture university of Thailand) and semi-wild red bean (Y001, introduce from agriculture university of Thailand) preparing hybrid combination, note doing female parent with Y001, Tr001 is male parent (reciprocal cross success rate only has orthogonal 10%).Obtain first familiar generation seed.The filial generation seed of results is carried out adding generation in Hainan winter, adopt nylon wire to hide isolated area method carries out anti-bean weevil qualification to above filial generation, it is for subsequent use that the individual plant filtering out high resistance bean weevil is left to lower season.
Anti-bean weevil qualification, with reference to the mung bean of ARC-AVRDC and the anti-bean weevil authenticate technology of Kidney bean of CIAT (CIAT), adopts indoors artificial to connect worm method.By each breeding lines and anti-, that the healthy seed of sense parent puts into Φ 6cm and H1cm respectively small plastic box, do not add a cover, put into large plastic casing (66cm × 44cm × 18cm) at random, upper cover two layers of black cloth, are placed on foster worm frame.Insectary temperature controls at 27 DEG C ± 2 DEG C, and keeps dark and relative high humidity.400 ~ 500 adults to the 1 ~ 3d that just sprouts wings are put in each large plastic casing, on average every part of identified material 8 is right, is allowed to condition in box and each material is laid eggs at random, ovum amount greatly about more than 5 time to sense worm parent every seed, taking out connecing worm material, removing all adults.After worm 40 ~ 45d waiting, investigate a number of being injured for every part of material, and evaluate its resistance class according to the rate of being injured, evaluation criterion is in table 1.
Table 1 red bean breeding lines resistance to insects evaluation criterion
B semi-wild anti-bean weevil red bean new germ plasm is hybridized with cultivation red bean
The anti-bean weevil semi-wild red bean new germ plasm that a upper season is obtained and Yangtze river basin main breed Su Hong No. 1 preparing hybrid combine.Anti-bean weevil selection being carried out respectively to offspring's individual plant of the hybrid combination obtained, staying standby rear use to screening the individual plant with above resistance obtained.
C obtains the red bean new germ plasm with anti-bean weevil characteristic by the method progressively backcrossed with conventional parent
By using No. 1, cultivar Su Hong as the female parent that backcrosses, with anti-bean weevil filial generation for male parent backcrosses, backcrossed for 5 generations to BC5 (adding generation in Hainan and greenhouse), per in generation, carries out with Phenotypic Selection, the methods such as laboratory qualification carry out comprehensive selection, differentiate backcross progeny, make it have anti-bean weevil characteristic, but other economical character and conventional parent backcross progeny closely.
Implementation result:
With the local red bean of routine in contrast, this patent method is adopted to obtain the cultivation red bean new germ plasm plantation results " Invest, Then Investigate " display of anti-bean weevil: worm cost 40-60 unit/mu controlled by the agricultural chemicals saving field, output increased more than 20%, after half a year is preserved in warehouse, rate of damaging by worms reduces by more than 15%.
The hybridization of the semi-wild that the embodiment of the present invention provides anti-bean weevil red bean obtains the method for anti-bean weevil red bean, makes male parent, semi-wild red bean makes female parent with anti-bean weevil rice bean, and hybridization obtains F1 generation seed, and goes out anti-bean weevil semi-wild red bean new germ plasm by anti-bean weevil evaluation and screening; Anti-bean weevil semi-wild red bean new germ plasm and conventional red bean kind are hybridized, anti-bean weevil qualification selection is carried out respectively, the individual plant with resistance that screening obtains to offspring's seed that hybridization obtains; Backcross for male parent using cultivar as the female parent that backcrosses, with anti-bean weevil filial generation, after 5 generations that backcrossed, differentiate backcross progeny, obtain the red bean kind with anti-bean weevil characteristic; The hybridization of this semi-wild anti-bean weevil red bean obtains the method for anti-bean weevil red bean, cost-saving, improve economic benefit, reduce residue of pesticide, ensure that eater's is healthy, extend red bean storage period, improve the marketing quality of red bean, effectively improve red bean commodity at home and abroad compete in status, there is stronger propagation and employment and be worth.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (1)
1. the hybridization of semi-wild anti-bean weevil red bean obtains a method for anti-bean weevil red bean, it is characterized in that, the method that the hybridization of this semi-wild anti-bean weevil red bean obtains anti-bean weevil red bean comprises the following steps:
Step one, makes male parent with anti-bean weevil rice bean, semi-wild red bean makes female parent, and hybridization obtains F1 generation seed, and goes out semi-wild anti-bean weevil red bean new germ plasm by anti-bean weevil evaluation and screening;
Step 2, anti-for semi-wild bean weevil red bean new germ plasm and conventional red bean kind are hybridized, conventional red bean kind is No. 1, Yangtze river basin main breed Su Hong, carries out anti-bean weevil qualification selection respectively, the individual plant with resistance that screening obtains to offspring's seed that hybridization obtains;
Step 3, backcrosses using No. 1, main breed Su Hong as the female parent that backcrosses, with anti-bean weevil filial generation for male parent, needs to add generation in Hainan and greenhouse, after 5 generations that backcrossed, differentiates backcross progeny, obtains the red bean kind with anti-bean weevil characteristic;
Anti-bean weevil qualification adopts indoors artificial to connect worm method, by each breeding lines and anti-, the healthy seed of sense parent puts into the small plastic box of Φ 6cm and H1cm respectively, do not add a cover, put into large plastic casing at random, upper cover two layers of black cloth, be placed on foster worm frame, insectary temperature controls at 27 DEG C ± 2 DEG C, and keep dark and relative high humidity, 400 ~ 500 adults to the 1 ~ 3d that just sprouts wings are put in each large plastic casing, on average every part of identified material 8 is right, in box, each material is laid eggs at random, to sense worm parent every seed ovum amount more than 5 time, take out connecing worm material, remove all adults, after worm 40 ~ 45d waiting, investigate a number of being injured for every part of material, and evaluate resistance class according to rate of being injured,
The evaluation criterion evaluating resistance class according to rate of being injured is:
When seed rate of damage is 0 ~ 10%, be then high resistance bean weevil kind, grade is 1 grade;
When seed rate of damage is 10.1 ~ 35%, be then anti-bean weevil kind, grade is 3 grades;
When seed rate of damage is 35.1 ~ 65%, then anti-bean weevil kind in being, grade is 5 grades;
When seed rate of damage is 65.1 ~ 90%, be then induction reactance bean weevil kind, grade is 7 grades;
When seed rate of damage is greater than 90%, be then high induction reactance bean weevil kind, grade is 9 grades.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310062684.3A CN103109735B (en) | 2013-02-28 | 2013-02-28 | Method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310062684.3A CN103109735B (en) | 2013-02-28 | 2013-02-28 | Method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103109735A CN103109735A (en) | 2013-05-22 |
| CN103109735B true CN103109735B (en) | 2015-04-08 |
Family
ID=48408122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310062684.3A Expired - Fee Related CN103109735B (en) | 2013-02-28 | 2013-02-28 | Method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103109735B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122322A (en) * | 2019-06-10 | 2019-08-16 | 江汉大学 | A method of cultivating cowpea new germ plasm |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101358232A (en) * | 2007-08-03 | 2009-02-04 | 中国农业科学院作物科学研究所 | Molecular marker linked with bruchid resistance gene in mung bean |
| CN101358233A (en) * | 2007-08-03 | 2009-02-04 | 中国农业科学院作物科学研究所 | QTL mapping range of bruchid resistance in mung bean |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004329010A (en) * | 2003-04-30 | 2004-11-25 | National Institute Of Agrobiological Sciences | Method for creating adzuki bean seed having resistance to bruchidae |
-
2013
- 2013-02-28 CN CN201310062684.3A patent/CN103109735B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101358232A (en) * | 2007-08-03 | 2009-02-04 | 中国农业科学院作物科学研究所 | Molecular marker linked with bruchid resistance gene in mung bean |
| CN101358233A (en) * | 2007-08-03 | 2009-02-04 | 中国农业科学院作物科学研究所 | QTL mapping range of bruchid resistance in mung bean |
Non-Patent Citations (4)
| Title |
|---|
| Tagging and Utilization Bruchid Resistance Gene Using PCR Markers in Mungbean;Cheng Xu-Zhen et al.;《Agricultural Sciences in China》;20051231;第4卷(第8期);第580页，右栏第4-30行 * |
| 江苏省外国专家局.抗豆象小豆分子标记的筛选研究技术交流.《海外高层次人才联系窗口,网址:http://www.chinajob.cn/jobs/detail.php?id=2473》.2007,第1页. * |
| 绿豆抗豆象育种后代 F2 群体遗传变异分析;程须珍等;《植物遗传资源学报》;20041231;第5卷(第4期);"1.2试验方法"节，表2 * |
| 远缘杂交获得抗豆象小豆新种质;陈新等;《江苏省遗传学会植物分子育种学术研讨会论文摘要汇编》;20070701;第74页摘要 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103109735A (en) | 2013-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104137764B (en) | The selection of high resistance naked body yellow leaf Characters of Giant Embryo Rice CMS line | |
| CN103918537A (en) | Processing method for Dendrobium officinale culture substrate | |
| CN103975843A (en) | Introduction aggregation breeding method of maize inbred line | |
| CN106665332A (en) | Method for carrying out recurrent selection breeding on water-saving and drought-resisting rice by utilizing dominant nuclear male sterile material | |
| CN103155808A (en) | Conjoined grafting method for plant meristem | |
| CN104221861B (en) | A kind of method utilizing embryo rescue to realize red bean and rice bean distant hybridization | |
| CN106035059A (en) | Method for artificially synthesizing novel anti-clubroot brassica napus material | |
| CN101361467B (en) | Common intertidal shellfish hybridization method | |
| CN105028184A (en) | Rice fragrant and soft rice variety selecting and culturing method | |
| CN106035177A (en) | Symbiotic planting and breeding method of soft-shelled turtles, eels, fish and lotuses | |
| CN103109735B (en) | Method for obtaining bruchid-resistant red beans by hybridization of semi-natural bruchid-resistant red beans | |
| CN105010126A (en) | Fast breeding method for black spot resistant sweet potato variety | |
| WO2010058399A1 (en) | Pest resistant plant | |
| CN1954666B (en) | Rice protection cytoplasmic male sterile line and recovery line directive breeding method | |
| Thakur et al. | Sorghum grain mold information Bulletin No. 72 | |
| CN106212036A (en) | A kind of method of the wild bean breeding progeny with corn intercropping plant | |
| CN106489727A (en) | A kind of bacterial Footrot resistance Japonica two line hybrid rice method | |
| CN109729934A (en) | A method of it is pollinated using fly auxiliary brassicaceous vegetable | |
| CN106134978B (en) | A kind of selection suitable for mulberry shoot harvesting mulberry tree breed | |
| Beatson et al. | Progress in the breeding of novel interspecific Actinidia hybrids | |
| Dhaliwal et al. | INTERTRAIT ASSOCIATIONS AND PATH ANALYSIS STUDIES IN GROUNDNUT(Arachis hypogaea L) | |
| CN104303992B (en) | A kind of breeding method of high mildew-resistance Fructus Momordicae charantiae | |
| CN107750939A (en) | A kind of rice cultivating method | |
| CN107410008A (en) | A kind of selection of resistance to lead Job's tears kind | |
| CN106212035A (en) | A kind of method of the wild bean breeding progeny with Sorghum vulgare Pers. intercropping plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150408 |