CN107410008A - A kind of selection of resistance to lead Job's tears kind - Google Patents
A kind of selection of resistance to lead Job's tears kind Download PDFInfo
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- CN107410008A CN107410008A CN201710373865.6A CN201710373865A CN107410008A CN 107410008 A CN107410008 A CN 107410008A CN 201710373865 A CN201710373865 A CN 201710373865A CN 107410008 A CN107410008 A CN 107410008A
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- 241000209205 Coix Species 0.000 title claims abstract description 53
- 235000007354 Coix lacryma jobi Nutrition 0.000 title claims abstract description 46
- 241000196324 Embryophyta Species 0.000 claims abstract description 26
- 230000035784 germination Effects 0.000 claims abstract description 17
- 238000009395 breeding Methods 0.000 claims abstract description 9
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000012216 screening Methods 0.000 claims abstract description 5
- 238000009396 hybridization Methods 0.000 claims abstract description 3
- 238000009399 inbreeding Methods 0.000 claims abstract description 3
- 230000001954 sterilising effect Effects 0.000 claims description 13
- RLJMLMKIBZAXJO-UHFFFAOYSA-N lead nitrate Chemical compound [O-][N+](=O)O[Pb]O[N+]([O-])=O RLJMLMKIBZAXJO-UHFFFAOYSA-N 0.000 claims description 12
- 239000004576 sand Substances 0.000 claims description 12
- 238000003306 harvesting Methods 0.000 claims description 11
- 238000005286 illumination Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- 230000005059 dormancy Effects 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000012549 training Methods 0.000 claims description 6
- 230000009418 agronomic effect Effects 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000002955 isolation Methods 0.000 claims description 5
- 230000008774 maternal effect Effects 0.000 claims description 5
- 238000009331 sowing Methods 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 4
- 238000013459 approach Methods 0.000 abstract description 2
- 239000013043 chemical agent Substances 0.000 abstract 1
- 230000010154 cross-pollination Effects 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 7
- 240000002853 Nelumbo nucifera Species 0.000 description 6
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 6
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 208000035240 Disease Resistance Diseases 0.000 description 3
- 241000167782 Lithospermum virginianum Species 0.000 description 3
- 208000036626 Mental retardation Diseases 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000010814 metallic waste Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of selection of resistance to lead Job's tears kind, the characteristics of it utilizes Job's tears cross-pollination heterozygosity high, parent is used as using two or more Job's tears kinds, after its natural hybridization, specific chemical agent is respectively adopted with seedling stage in the germination period of seed obtained by female parent to be handled, with screening obtain with lead resistance can specific plant, then after plant is planted it is homozygous through inbreeding of more generation, obtain the resistance to lead Job's tears kind that botany dimensionally stable, character are unified, yield is suitable.The inventive method can shorten the seed selection time of Job's tears kind, improve the Breeding Efficiency of the resistance to lead kind of Job's tears, and a kind of efficient, reliable approach is provided for the seed selection of resistance to lead Job's tears kind.
Description
Technical field
The invention belongs to crop breeding technique field, and in particular to a kind of selection of resistance to lead Job's tears kind.
Background technology
With China's expanding economy, heavy metal waste pollution has become the major issue for endangering health of people.According to report
Road, in China's cultivated area, heavy metal pollution area nearly 20,000,000 hm2, account for the whole nation 1/5.It is heavy metal-polluted in soil
The more difficult movement of thing is contaminated, is easily detained, can also be transmitted by water and the plant being grown in soil, serious threat the mankind's
Health.
One of most common and stronger heavy metal of toxic is exactly lead in environment, and heavy metal lead is to the very harmful of human body.
A large amount of use of the development of modern industry and transportation in recent years and agrochemical fertilizer make it that the lead contamination in soil is more next
It is more serious, after the lead in soil is absorbed by plants, human body is entered by food chain, be its important channel for being detrimental to health it
One.Lead in nature by alimentary canal in addition to entering human body, moreover it is possible to enters human body by respiratory tract and skin, to the god of people
The harm of lifelong participation is caused, and body's immunity can be reduced through, the system such as blood, angiocarpy, causes hepatic sclerosis or hepatonecrosis,
Mental retardation of children is caused what is more.
The content of the invention
It is an object of the invention to provide a kind of selection of resistance to lead Job's tears kind, this method can quick breeding go out it is resistance to
The Job's tears new varieties of lead, significantly improve the resistance to lead ability of Job's tears.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of selection of resistance to lead Job's tears kind, it is using two or more Job's tears kinds as parent, by its natural hybridization
Afterwards, lead nitrate solution is respectively adopted with seedling stage is handled in the germination period of seed obtained by female parent, being obtained with screening has resistance to lead
The plant of performance, then the resistance to lead Job's tears self-mating system stablized after plant is planted through inbreeding of more generation homozygosis, acquisition Agronomic trait；Its
Comprise the following steps：
A, hybridize：
1）Using two or more Job's tears kinds as parent；
2）Arrange to sow according to the breeding time of Job's tears kind used, the flowering time interval of different cultivars Job's tears used is less than 7
My god；
3）Post flowering, the kind wherein as female parent is removed into stamen, male parent, female parent is freely hybridized；
4）Divide male parent, maternal progress sowing；
5）The F1 seeds that Second Year plantation harvests from female parent, and isolation is performed, to harvest F2 seeds；
B, germination period is handled：
1）By gained F2 seeds in toasting 4h in 38 DEG C of drying bakers, with Dormancy breaking；
2）The seed after dormancy will be cracked and sterilize 20min with 5vol% liquor natrii hypochloritises, then with deionized water rinsing 3-5 times,
Again surface moisture is blotted with filter paper；
3）Seed after sterilization is placed in and is lined with the germination box of one layer of filter paper, per box seed by be paved with cassette bottom, it is not overlapping be defined,
And the lead nitrate solution for adding 1000mg/L extremely submerges the surface of the seed as nutrient solution as degree, is subsequently placed in 28-30 DEG C of illumination training
Support in case, daily illumination 8h carries out vernalization；1 nutrient solution is changed in pregermination procedure daily, by seed deionized water before changing
Rinse 3 times, and the surface of the seed moisture is blotted with blotting paper；Germination box used need to be sterilized with 75 vol% alcohol wipes in advance, filter paper
Sterilized with high-pressure sterilizing pot；
C, seedling stage is handled：
Germinate after 7-10d, normal seedling individual plant will be grown and be transplanted to using river sand in the flowerpot of matrix, to use matrix volume daily
10% 200mg/L lead nitrate solution is poured, and is screened normal growth and can be extracted the plant of young leaves out, that is, obtaining has lead resistance
The coix plant of energy；River sand used is through sterilization in advance；
D, selfing is homozygous：
1）The coix plant with lead resistance energy of acquisition is transplanted to crop field and planted；
2）Bagging is carried out before plant blossom, in favor of selfing, obtains selfed seed；
3）The lead content in seed is determined, filters out the self-mating system that lead tolerance is not higher than 0.2mg/kg；
4）Selected self-mating system is pressed into step 1 under D items）-3）Repeated, it is selfed 6-9 generations, obtain botany
The stable resistance to lead Job's tears self-mating system of shape.
The remarkable advantage of the present invention is：The present invention can substantially shorten the seed selection time of Job's tears kind, improve the resistance to lead of Job's tears
The Breeding Efficiency of kind, a kind of efficient, reliable approach is provided for the seed selection of resistance to lead Job's tears kind.
Embodiment
In order that content of the present invention easily facilitates understanding, with reference to embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
By with it is temperature automatically controlled, control it is wet, control illumination and ventilation condition incubator and field test condition exemplified by, using training
Support case and carry out bud seedling screening, crop field is hybridized, is selfed, be homozygous with screening.
Embodiment 1
A, hybridize：
1）With the Pu heart of a lotus seed, No. 6 are female parent, and wild Job's tears is male parent；
2）Arrange to sow according to the breeding time of Job's tears kind used, the flowering time interval of different cultivars Job's tears used is less than 7
My god；
3）Post flowering, the stamen of female parent is removed, male parent, female parent is freely hybridized；
4）Divide male parent, maternal progress sowing；Dry, store；
5）The F1 seeds that Second Year plantation harvests from female parent, and isolation is performed, to harvest F2 seeds；Gained F2 seeds dry standby
With；
B, germination period is handled：
1）By gained F2 seeds in toasting 4h in 38 DEG C of drying bakers, with Dormancy breaking；
2）The seed after dormancy will be cracked and sterilize 20min with 5vol% liquor natrii hypochloritises, then with deionized water rinsing 3 times, then
Surface moisture is blotted with filter paper；
3）Seed after sterilization is placed in and is lined with the germination box of one layer of filter paper, per box seed by be paved with cassette bottom, it is not overlapping be defined,
And the lead nitrate solution for adding 1000mg/L extremely submerges the surface of the seed as nutrient solution as degree, is subsequently placed in 28-30 DEG C of illumination training
Support in case, daily illumination 8h carries out vernalization；1 nutrient solution is changed in pregermination procedure daily, by seed deionized water before changing
Rinse 3 times, and the surface of the seed moisture is blotted with blotting paper；Germination box used need to be sterilized with 75 vol% alcohol wipes in advance, filter paper
Sterilized with high-pressure sterilizing pot；Mouldy rotten seed is removed in time；
C, seedling stage is handled：
Germinate after 7d, normal seedling individual plant will be grown and be transplanted in the flowerpot equipped with river sand, daily with river sand volume 10%
200mg/L lead nitrate solution is poured, and is screened normal growth and can be extracted the plant of young leaves out, that is, obtains the Job's tears with lead resistance energy
Plant；River sand used is through sterilization in advance；
D, selfing is homozygous：
1）The coix plant with lead resistance energy of acquisition is transplanted to crop field and planted；
2）Field carries out Disease Resistance Identification and Yield comparison；
3）Bagging is carried out before plant blossom, in favor of selfing, obtains selfed seed；
4）The lead content in seed is determined, lead tolerance is filtered out and is less than national standard（≤0.2mg/kg）Self-mating system；
5）Selected self-mating system is pressed into step 1 under D items）-4）Repeated, it was selfed for 6 generations, obtain Agronomic trait
Stable resistance to lead Job's tears self-mating system.
The resistance to lead Job's tears self-mating system of gained is planted in high lead soil to, after testing, its yield and the Pu heart of a lotus seed 6 as female parent
Without significant difference, gained seed lead tolerance is not higher than 0.2mg/kg, it was demonstrated that and it has good lead resistance energy, and through planting for many years,
Characters of progenies is stable.
Embodiment 2
A, hybridize：
1）It is female parent with the peaceful heart of a lotus seed 1, wild Job's tears is male parent；
2）Arrange to sow according to the breeding time of Job's tears kind used, the flowering time interval of different cultivars Job's tears used is less than 7
My god；
3）Post flowering, the stamen of female parent is removed, male parent, female parent is freely hybridized；
4）Divide male parent, maternal progress sowing；Dry, store；
5）The F1 seeds that Second Year plantation harvests from female parent, and isolation is performed, to harvest F2 seeds；Gained F2 seeds dry standby
With；
B, germination period is handled：
1）By gained F2 seeds in toasting 4h in 38 DEG C of drying bakers, with Dormancy breaking；
2）The seed after dormancy will be cracked and sterilize 20min with 5vol% liquor natrii hypochloritises, then with deionized water rinsing 4 times, then
Surface moisture is blotted with filter paper；
3）Seed after sterilization is placed in and is lined with the germination box of one layer of filter paper, per box seed by be paved with cassette bottom, it is not overlapping be defined,
And the lead nitrate solution for adding 1000mg/L extremely submerges the surface of the seed as nutrient solution as degree, is subsequently placed in 28-30 DEG C of illumination training
Support in case, daily illumination 8h carries out vernalization；1 nutrient solution is changed in pregermination procedure daily, by seed deionized water before changing
Rinse 3 times, and the surface of the seed moisture is blotted with blotting paper；Germination box used need to be sterilized with 75 vol% alcohol wipes in advance, filter paper
Sterilized with high-pressure sterilizing pot；Mouldy rotten seed is removed in time；
C, seedling stage is handled：
Germinate after 8d, normal seedling individual plant will be grown and be transplanted in the flowerpot equipped with river sand, daily with river sand volume 10%
200mg/L lead nitrate solution is poured, and is screened normal growth and can be extracted the plant of young leaves out, that is, obtains the Job's tears with lead resistance energy
Plant；River sand used is through sterilization in advance；
D, selfing is homozygous：
1）The coix plant with lead resistance energy of acquisition is transplanted to crop field and planted；
2）Field carries out Disease Resistance Identification and Yield comparison；
3）Bagging is carried out before plant blossom, in favor of selfing, obtains selfed seed；
4）The lead content in seed is determined, lead tolerance is filtered out and is less than national standard（≤0.2mg/kg）Self-mating system；
5）Selected self-mating system is pressed into step 1 under D items）-4）Repeated, it was selfed for 8 generations, obtain Agronomic trait
Stable resistance to lead Job's tears self-mating system.
The resistance to lead Job's tears self-mating system of gained is planted in high lead soil to, after testing, its yield and the peaceful heart of a lotus seed 1 as female parent
Without significant difference, gained seed lead tolerance is not higher than 0.2mg/kg, it was demonstrated that and it has good lead resistance energy, and through planting for many years,
Characters of progenies is stable.
Embodiment 3
A, hybridize：
1）It is female parent with the imperial heart of a lotus seed 1, wild Job's tears is male parent；
2）Arrange to sow according to the breeding time of Job's tears kind used, the flowering time interval of different cultivars Job's tears used is less than 7
My god；
3）Post flowering, the stamen of female parent is removed, male parent, female parent is freely hybridized；
4）Divide male parent, maternal progress sowing；Dry, store；
5）The F1 seeds that Second Year plantation harvests from female parent, and isolation is performed, to harvest F2 seeds；Gained F2 seeds dry standby
With；
B, germination period is handled：
1）By gained F2 seeds in toasting 4h in 38 DEG C of drying bakers, with Dormancy breaking；
2）The seed after dormancy will be cracked and sterilize 20min with 5vol% liquor natrii hypochloritises, then with deionized water rinsing 5 times, then
Surface moisture is blotted with filter paper；
3）Seed after sterilization is placed in and is lined with the germination box of one layer of filter paper, per box seed by be paved with cassette bottom, it is not overlapping be defined,
And the lead nitrate solution for adding 1000mg/L extremely submerges the surface of the seed as nutrient solution as degree, is subsequently placed in 28-30 DEG C of illumination training
Support in case, daily illumination 8h carries out vernalization；1 nutrient solution is changed in pregermination procedure daily, by seed deionized water before changing
Rinse 3 times, and the surface of the seed moisture is blotted with blotting paper；Germination box used need to be sterilized with 75 vol% alcohol wipes in advance, filter paper
Sterilized with high-pressure sterilizing pot；Mouldy rotten seed is removed in time；
C, seedling stage is handled：
Germinate after 10d, normal seedling individual plant will be grown and be transplanted in the flowerpot equipped with river sand, daily with river sand volume 10%
200mg/L lead nitrate solution is poured, and is screened normal growth and can be extracted the plant of young leaves out, that is, obtains the Job's tears with lead resistance energy
Plant；River sand used is through sterilization in advance；
D, selfing is homozygous：
1）The coix plant with lead resistance energy of acquisition is transplanted to crop field and planted；
2）Field carries out Disease Resistance Identification and Yield comparison；
3）Bagging is carried out before plant blossom, in favor of selfing, obtains selfed seed；
4）The lead content in seed is determined, lead tolerance is filtered out and is less than national standard（≤0.2mg/kg）Self-mating system；
5）Selected self-mating system is pressed into step 1 under D items）-4）Repeated, it was selfed for 9 generations, obtain Agronomic trait
Stable resistance to lead Job's tears self-mating system.
The resistance to lead Job's tears self-mating system of gained is planted in high lead soil to, after testing, its yield and the imperial heart of a lotus seed 1 as female parent
Without significant difference, gained seed lead tolerance is not higher than 0.2mg/kg, it was demonstrated that and it has good lead resistance energy, and through planting for many years,
Characters of progenies is stable.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (2)
1. a kind of selection of resistance to lead Job's tears kind, it is characterised in that using two or more Job's tears kinds as parent, by it
After natural hybridization, lead nitrate solution is respectively adopted in germination period and the seedling stage of seed obtained by female parent and is handled, obtained with screening
Plant with lead resistance energy, then the resistance to lead Job's tears stablized after plant is planted through inbreeding of more generation homozygosis, acquisition Agronomic trait
Self-mating system.
2. the selection of resistance to lead Job's tears kind according to claim 1, it is characterised in that comprise the following steps：
A, hybridize：
1）Using two or more Job's tears kinds as parent；
2）Arrange to sow according to the breeding time of Job's tears kind used, the flowering time interval of different cultivars Job's tears used is less than 7
My god；
3）Post flowering, the kind wherein as female parent is removed into stamen, male parent, female parent is freely hybridized；
4）Divide male parent, maternal progress sowing；
5）The F1 seeds that Second Year plantation harvests from female parent, and isolation is performed, to harvest F2 seeds；
B, germination period is handled：
1）By the F2 seeds of harvest in toasting 4h in 38 DEG C of drying bakers, with Dormancy breaking；
2）The seed after dormancy will be cracked and sterilize 20min with 5vol% liquor natrii hypochloritises, then with deionized water rinsing 3-5 times,
Again surface moisture is blotted with filter paper；
3）Seed after sterilization is placed in and is lined with the germination box of one layer of filter paper, per box seed by be paved with cassette bottom, it is not overlapping be defined,
And the lead nitrate solution for adding 1000mg/L extremely submerges the surface of the seed as nutrient solution as degree, is subsequently placed in 28-30 DEG C of illumination training
Support in case, daily illumination 8h carries out vernalization；1 nutrient solution is changed in pregermination procedure daily, by seed deionized water before changing
Rinse 3 times, and the surface of the seed moisture is blotted with blotting paper；
C, seedling stage is handled：
Germinate after 7-10d, normal seedling individual plant will be grown and be transplanted to using river sand in the flowerpot of matrix, to use matrix volume daily
10% 200mg/L lead nitrate solution is poured, and is screened normal growth and can be extracted the plant of young leaves out, that is, obtaining has lead resistance
The coix plant of energy；
D, selfing is homozygous：
1）The coix plant with lead resistance energy of acquisition is transplanted to crop field and planted；
2）Bagging is carried out before plant blossom, in favor of selfing, obtains selfed seed；
3）The lead content in seed is determined, filters out the self-mating system that lead tolerance is not higher than 0.2mg/kg；
4）Selected self-mating system is pressed into step 1 under D items）-3）Repeated, it is selfed 6-9 generations, obtain botany
The stable resistance to lead Job's tears self-mating system of shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710373865.6A CN107410008A (en) | 2017-05-24 | 2017-05-24 | A kind of selection of resistance to lead Job's tears kind |
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| CN201710373865.6A CN107410008A (en) | 2017-05-24 | 2017-05-24 | A kind of selection of resistance to lead Job's tears kind |
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| CN107410008A true CN107410008A (en) | 2017-12-01 |
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| CN201710373865.6A Pending CN107410008A (en) | 2017-05-24 | 2017-05-24 | A kind of selection of resistance to lead Job's tears kind |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109892175A (en) * | 2019-01-09 | 2019-06-18 | 云南省农业科学院经济作物研究所 | A kind of identification method of anti-plumbing hemp germ plasm resource |
-
2017
- 2017-05-24 CN CN201710373865.6A patent/CN107410008A/en active Pending
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| Title |
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| 丁显萍等: "解除水稻种子休眠方法探讨", 《安徽农学通报》 * |
| 夏法刚等: "铜胁迫对薏苡种子萌发及幼苗生长的影响研究", 《中国种业》 * |
| 李容等: "石墨炉原子吸收法测定薏苡仁中4 种重金属含量", 《广西工学院学报》 * |
| 覃初贤等: "薏苡种子发芽技术", 《种子世界》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109892175A (en) * | 2019-01-09 | 2019-06-18 | 云南省农业科学院经济作物研究所 | A kind of identification method of anti-plumbing hemp germ plasm resource |
| CN109892175B (en) * | 2019-01-09 | 2021-11-05 | 云南省农业科学院经济作物研究所 | Identification method of lead-resistant industrial hemp germplasm resource |
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