CN103190381B - Double back hands over the breeding method of selection and breeding of silkworm multiple cross kind - Google Patents
Double back hands over the breeding method of selection and breeding of silkworm multiple cross kind Download PDFInfo
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- CN103190381B CN103190381B CN201310154693.5A CN201310154693A CN103190381B CN 103190381 B CN103190381 B CN 103190381B CN 201310154693 A CN201310154693 A CN 201310154693A CN 103190381 B CN103190381 B CN 103190381B
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Abstract
The invention provides the breeding method that a kind of double back hands over selection and breeding of silkworm multiple cross kind, select a two sex-limited Quaternary hybrid to be carrier kind.First by continuous backcross after the basic material of a resistance and carrier inter breed crossing, after by the basic material of an excellent silk quality again with first hybridize the identical carrier inter breed crossing importing resistant gene, then continuous backcross, obtain existing resistance, have the purebred of excellent silk quality again, through coordinate force test, finally cultivate into the practical kind of multiple cross.
Description
Technical field
The present invention relates to the breeding method of a kind of silkworm, particularly relate to a kind of multicomponent cross breeding method handing over selection and breeding of silkworm with double back.
Background technology
Crossbreeding uses the breeding method the most general, technology is the most ripe in breeding method, and in silkworm breeding, application is very extensive, be also a kind ofly constantly to make a breakthrough, technology that new method emerges in an endless stream.
The present invention selects breeding material, be dapple two sex-limited, the kind of growing fast, wide adaptability of band, simultaneously select 4 of the same hybrid combination of production purebred be carrier kind, namely select to produce upper to use but some proterties needs the Quaternary hybrid kind of improvement: MN × ST, integrated mode be in × everyday.Also be the middle in pairs system and Japanese kind selecting coordinate force good with the importing basic material that it carries out double back friendship, the object of this Selection parent is after being handed over by double back, can on the basis that coordinate force is good, the objective trait of needs can be obtained as early as possible, make seed selection material when importing two kind genes, obtain commercial variety rapidly.
The seed selection of current cultivated silkworm breed variety all adopts hybridization technique usually, a kind of specific gene of kind can be imported in another kind, makes integral economic trait reach practical level.But identical due to kind material, the kind namely using cross method to be bred as also difficulty also reaches the better level of integral economic trait, and for this reason, breeding scholar solves the deficiency existed in breeding work seeking various method.
Summary of the invention
The technical problem to be solved in the present invention is, provides a kind of double back to hand over the breeding method of selection and breeding of silkworm multiple cross kind, and it finally can obtain resistance, silk quality is excellent, coordinate force is good practical new varieties.
The present invention is achieved in that double back hands over the breeding method of selection and breeding of silkworm multiple cross kind, comprises the following steps:
(1) a Quaternary hybrid MN × ST with the two sex-limited characteristic of speckle is selected to be carrier kind.
(2) with being that purebred (M, N) hybridizes in basic material A and two of resistance, (M × A) F is obtained
1M × A(N × A) F
1M × Acrossbreed;
(3) hybridize with resistance basic material B and two Japanese purebred (S, T), obtain (S × B) F
1S × B(T × B) F
1T × Bcrossbreed;
(4) F of step (1) and step (2) is used
1crossbreed and corresponding basic material A, B continuous backcross, until obtain target resistance kind, be designated as MA, NA, SB, TB respectively;
(5) the basic material C using silk quality excellent again be that resistance kind MA, NA are hybridized in step (3) obtains, obtain middle system (MA × C) F
1(MA × C), (NA × C) F
1(NA × C);
(6) hybridize with Japanese resistance kind SB, the TB obtained through step (3) with the basic material D that silk quality is excellent, obtain Japanese (SB × D) F
1(SB × D)(TB × D) F
1(TB × D);
(7) with the F that step (4) and step (5) obtain
1crossbreed and corresponding basic material C, D continuous backcross, until obtain excellent silk quality kind on the basis of resistance, be designated as MAC, NAC, SBD, TBD respectively.
At least continuous backcross more than 5 times in step (4).
F described in step (4) and step (7)
1crossbreed is double back friendship kind.First educate resistance, after to educate silk quality excellent.Because silkworm resistance is by controlled by multiple genes-" Chinese Silkworm Egg Science " P247, the kind material of first seed selection resistance, the upper hybridization in this basis imports excellent silk quality gene, limit educate silk quality excellent while, utilize keep resistance summer and autumn, target can be reached within the shortest time like this.Vice versa, just selects in emphasis different in system of selection and spring and autumn.
The double back of described selection and breeding of silkworm hands over breeding method, and described basic material kind belongs to identical strain (i.e. Chinese strain kind and Chinese strain inter breed crossing, Japanese strain kind and Japanese strain inter breed crossing) with cultivation resistance or silk quality kind material.
The basic material (A, B) of described twice and (C, D) are has the good middle in pairs system of coordinate force and Japanese kind.
By breeding method after the individual step in (1)-(7), set up multiple system, remain (in × everyday) Quaternary hybrid pattern through selection, directive breeding, coordinate force test, assembly Combination nova (new varieties) four improved seeds MAC, NAC, SBD, TBD obtaining.
The coordinate force of homology original seed in polynary kind should be test in the seed selection of polynary kind, also to test polynary kind to the coordinate force handing over original seed simultaneously, therefore, the indices balanced growth of polynary kind also will be focused in seed selection overall process, this is different from the seed selection of binary kind from difficulty larger part, is excellent in existing crossbreed with polynary kind.The present invention carries out two excellent new silkworm variety of backcross breeding to multiple cross kind, and breeding goal obtains resistance, the excellent new silkworm variety of silk quality.
The basic material selected in the present invention is kind that is paired, resistance, and the carrier kind of selection is a two sex-limited Quaternary hybrid, with speckle feature assisted Selection, accelerates and is bred as target variety process.First educate resistance, after to educate silk quality excellent, simultaneously because selecting from same combination kind, its coordinate force is selected to obtain the good target variety of coordinate force at short notice by retentivity in breeding process, thus ensure that purity and the hybrid rate of breeding.
The double back that the present invention adopts hands over selection and breeding of silkworm kind to be also a kind of new try.The technology new opplication of a kind of crossbreeding that what double back handed over selection and breeding of silkworm polyspecific hybrids is, it is improved by cross breeding method, is applied to silkworm breeding field, and show the superiority of its uniqueness.Find and excellent silk quality and resistance reasonable combination, in the hope of reaching the excellent new varieties of incubation existing resistance, silk quality.
The explanation of part term in the present invention:
(1) basic material-resistance of resistance refers to a kind of defense response that HOST ORGANISMS is attacked pathogen, harmful substance and unsuitable environmental condition, and it is determined by the genotype of organism, is a kind of hereditary capacity.The basic material of resistance is exactly the basic silkworm race with resistance trait, and be also usually says purebred, this describing just is convenient to distinguish with the kind usually said (kind that production uses is hybrid combination).(" Chinese Silkworm Egg Science " P245)
The excellent excellent finger of basic material-silk quality of silk quality relative to other kind with strain at filament length, length of non broken filament, cleanliness, the main silk quality proterties comparatively quality product kind material such as clean.The excellent basic material of silk quality is exactly have the excellent basic silkworm race of silk quality achievement, is also usually say purebred.
(3) in be purebred-refer to Chinese system cultivated silkworm breed variety.
(4) Japanese purebred-in a few days native system cultivated silkworm breed variety.
(5) target resistance kind-refer to take resistance as the kind of breeding objective
(6) excellent silk quality kind-with the basic material of excellent silk quality is identical concept.
(7) two sex-limited-also claim speckle two sex-limited, refer to the middle system of silkworm, the larva (4,5 age silkworm just obviously can show the sex-limited characteristic of speckle) of the original seed level of Japanese kind and crossbreed level be the sex-limited proterties of male and female speckle.
In addition, the basic material selected due to the present invention is a two sex-limited Quaternary hybrid, and the new varieties be bred as after double cross are the bases of carrying out speckle marker-assisted breeding (can make molecular marker breeding again).Marker-assisted breeding is that biotechnology combines with traditional genetic breeding and formed, it can reduce the Linkage drag being difficult in traditional back cross breeding process eliminate, can also the gene pyramiding of various trait to remaining two sex-limited proterties equally, thus enhance the productivity being bred as new varieties, can male and female be divided to raise by silkworm rearing season in original silkworm rearing, also male and female can be divided to raise in the raw cocoon production phase, improve the scientific and technological content of new varieties.
Embodiment
Embodiment 1: double back hands over the breeding method of selection and breeding of silkworm multiple cross kind:
Hybrid combination before breeding is: this (in × everyday) Quaternary hybrid pattern being silkworm of (MN) × (ST).
1, breeding process is
(1) (M × A) to backcross after more than A five generation × C
(2) (N × A) to backcross after more than A five generation × C
(3) (S × B) to backcross after more than B five generation × D
(4) (T × B) to backcross after more than B five generation × D
Wherein there are twice hybridization, continuous backcross, set up multiple system, selection, directive breeding, coordinate force test, assembly Combination nova (new varieties).
2, be finally bred as:
﹛ (M × A) × C(N × A) × C) ﹜ × ﹛ (S × B) × D(T × B) × D) ﹜, Quaternary hybrid pattern that this combination of incubation remains (in × everyday).The kind be bred as combines the two sex-limited characteristic of speckle of carrier kind, coordinate force is good and acceptor varietal resistance is strong, silk quality is excellent proterties.
Claims (5)
1. double back hands over a breeding method for selection and breeding of silkworm multiple cross kind, it is characterized in that: comprise the following steps:
(1) a Quaternary hybrid MN × ST with the two sex-limited characteristic of speckle is selected to be carrier kind;
(2) with being that purebred M, N are hybridized in two of the basic material A of resistance and step (1), (M × A) F
1M × A(N × A) F
1N × Acrossbreed;
(3) hybridize with the basic material B of resistance and two Japanese purebred S, T of step (1), obtain (S × B) F
1S × B(T × B) F
1T × Bcrossbreed;
(4) F of step (2) and step (3) is used
1crossbreed and corresponding basic material A, B continuous backcross, until obtain target resistance kind, be designated as MA, NA, SB, TB respectively;
(5) the basic material C using silk quality excellent again be that resistance kind MA, NA are hybridized in step (4) obtains, obtain middle system F
1crossbreed (MA × C) F
1(MA × C), (NA × C) F
1(NA × C);
(6) the basic material D using silk quality excellent is again hybridized with Japanese resistance kind SB, the TB obtained through step (4), obtains Japanese F
1crossbreed (SB × D) F
1(SB × D)(TB × D) F
1(TB × D);
(7) with the F that step (5) and step (6) obtain
1crossbreed and corresponding basic material C, D continuous backcross, until obtain excellent silk quality kind on the basis of resistance, be designated as MAC, NAC, SBD, TBD respectively.
2. double back according to claim 1 hands over the breeding method of selection and breeding of silkworm multiple cross kind, it is characterized in that: at least continuous backcross more than 5 times in step (4) and step (7).
3. double back according to claim 1 hands over the breeding method of selection and breeding of silkworm multiple cross kind, it is characterized in that: described F
1crossbreed, step (4) is backcross first time, and step (7) is for backcrossing for the second time-planting referred to as double back friendship.
4. double back according to claim 1 hands over the breeding method of selection and breeding of silkworm multiple cross kind, it is characterized in that: the carrier kind of selection be same production uses to have four of the good breed combination of coordinate force purebred, there is the two sex-limited characteristic of speckle simultaneously.
5. double back according to claim 1 hands over the breeding method of selection and breeding of silkworm multiple cross kind, it is characterized in that: basic material kind belongs to identical strain with cultivation resistance or silk quality kind material, i.e. Chinese strain kind and Chinese strain inter breed crossing, Japanese strain kind and Japanese strain inter breed crossing.
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| CN103190381B true CN103190381B (en) | 2015-12-02 |
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| CN104430214B (en) * | 2014-12-31 | 2016-08-24 | 广东省蚕业技术推广中心 | A kind of robustness is from breeding method and the application going out the double sex-limited cultivated silkworm breed variety of cocoon speckle |
| CN107318790B (en) * | 2017-08-03 | 2019-06-28 | 广东省蚕业技术推广中心(广东省蚕业产品检测中心) | A kind of breeding method of the sex-limited cultivated silkworm breed variety of yellowish green cocoon of robustness and application |
| CN107821342B (en) * | 2017-11-30 | 2020-04-14 | 贵州省蚕业研究所 | Breeding method of silkworm with multiple silks and capable of resisting blood type pyosis |
| CN108849773B (en) * | 2018-06-25 | 2021-01-26 | 湖南省蚕桑科学研究所 | Transformation method for enabling stripes of silkworm daily-series tea-dot-restricted variety to be clearer |
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| 柞蚕新品种选育研究进展;朱有敏 等;《北方蚕业》;20120930;第33卷(第3期);第5-7页 * |
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Effective date of registration: 20180709 Address after: 551700 the first pioneer park of small and micro enterprises in Qixing District, Bijie, Guizhou Patentee after: BIJIE HUIJIANG SILKWORM DEVELOPMENT CO., LTD. Address before: 563000 new town, Guiyang, Guizhou Province, Zunyi Patentee before: Guizhou Province Silkworm Research Institute |
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Effective date of registration: 20210202 Address after: 550025 Jinzhu Town, Huaxi District, Guiyang City, Guizhou Province Patentee after: GUIZHOU SILKWORM Research Institute Address before: 551700 the first pioneer park of small and micro enterprises in Qixing District, Bijie, Guizhou Patentee before: BIJIE HUIJIANG SILKWORM DEVELOPMENT Co.,Ltd. |
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