CN103299898A - Molecular marker-assisted cultivating method of new high-digestibility sudangrass strain - Google Patents
Molecular marker-assisted cultivating method of new high-digestibility sudangrass strain Download PDFInfo
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- CN103299898A CN103299898A CN2013102817008A CN201310281700A CN103299898A CN 103299898 A CN103299898 A CN 103299898A CN 2013102817008 A CN2013102817008 A CN 2013102817008A CN 201310281700 A CN201310281700 A CN 201310281700A CN 103299898 A CN103299898 A CN 103299898A
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Abstract
A molecular marker-assisted cultivating method of a new high-digestibility sudangrass strain belongs to a superior sudangrass strain cultivating technology. According to the method, on the basis of the conventional cultivating methods of hybridization, backcrossing, selfing and the like, and a bmr-6 gene-carrying single plant can be selected in advance by utilizing a molecular marker identification method, thus the breeding process can be quickened. The molecular marker-assisted cultivating method comprises the steps of high-digestibility strain hybridization, three turns of backcrossing and three-time molecular marker identification, as well as the last time of selfing selection. The method is simple and easy to operate, and the breeding process can be quickened.
Description
Technical field
The auxiliary breeding method of a kind of molecular labeling of high digestibility Sudan grass new lines belongs to improved crop variety cultivation technology, says more specifically a kind of method of assisting to cultivate high digestibility Sudan grass new lines by molecular labeling.
Background technology
Arteries and veins is the variation phenomenon of a kind of vein and cane xylem in the Chinese sorghum brown.This mutant is compared digestibility than common White midrib Chinese sorghum and is improved 40%-60%, and palatability is obviously improved, and various pasture stocks are liked food especially, and same scale of feeding can obtain higher economic benefit.The arteries and veins type has kind more than 30 in the brown, and wherein digestibility the highest be the bmr-6 type.The method of arteries and veins (bmr-6 type) Sudan grass is to utilize the Chinese sorghum strain of arteries and veins in the brown and common Sudan grass strain to hybridize in the conventional cultivation brown, selects by arteries and veins phenotypic characteristic in its offspring's the brown.Because arteries and veins is recessive character in the brown, therefore when importing this proterties in Sudan grass by the mode that backcrosses, need hybridization 1 time, 3 times, selfing 3 times backcross.This method required time is long and time-consuming, also needs for 3 years even add the adding generation in winter in Hainan Island.
Summary of the invention
The objective of the invention is the induce one process of crossbreeding of the method that molecular labeling is auxiliary, cultivate the Sudan grass new lines of high digestibility, to accelerating the process of breeding, improve the operating efficiency of breeding.The present invention is achieved in that
1. the auxiliary breeding method of the molecular labeling of one kind high digestibility Sudan grass new lines is characterized in that, it comprises the steps:
1. high digestibility incross: utilize and contain the bmr-6 gene, its marker trait is the Sudan grass incross of arteries and veins in the Chinese sorghum strain of arteries and veins in the brown and the non-brown;
2. backcross for the first time: take crossbreed as maternal, the Sudan grass of arteries and veins is that male parent backcrosses in the non-brown, obtains BC1F1;
3. offspring's individual plant molecular markers for identification: at BC1F1 from generation to generation, minute individual plant extracts DNA, the individual plant that carries arteries and veins gene in the brown is carried out pcr amplification identify;
4. backcross for the second time: take the individual plant that carries the bmr-6 gene as maternal, the Sudan grass product of arteries and veins are the male parent BC2F1 that backcrosses to get in the non-brown;
5. molecular markers for identification and backcrossing for the third time for the second time: utilization and step 3. identical method identify the individual plant that carries the bmr-6 gene and are female parent, and the Sudan grass product of arteries and veins are the male parent BC3F1 that backcrosses to get in the non-brown;
6. for the third time molecular markers for identification and self cross and selection: utilize with step 3. identical method identify the then selfing of BC3F1 plant of carrying the bmr-6 gene, the individual plant that selection has an arteries and veins phenotype in the brown in the self progeny can obtain the Sudan grass new lines of high digestibility.
Beneficial effect of the present invention: the present invention hybridization, backcross and the basis of the breeding method such as selfing on, added the molecular markers for identification technology, to selecting in advance with the bmr-6 gene, therefore can accelerate breeding process.This method is easy and simple to handle, easy, can accelerate breeding process.This method also can be used for the cultivation of high digestibility Chinese sorghum strain.
Embodiment:
Below in conjunction with instantiation the present invention is conducted further description.
Embodiment 1: the molecular labeling of the Sudan grass strain of high digestibility is auxiliary to be cultivated
1. high digestibility incross: utilize arteries and veins Chinese sorghum strain U68(bmr-6 type in the brown) with Sudan grass strain S722 hybridization.
2. backcross for the first time: take crossbreed as maternal, Sudan grass strain S722 is that male parent backcrosses, and obtains BC1F1.
3. offspring's individual plant molecular markers for identification: BC1F1 plants 20 strains from generation to generation, and minute individual plant extracts DNA, the individual plant that carries arteries and veins gene in the brown is carried out pcr amplification identify.
4. backcross for the second time: take the individual plant that carries the bmr-6 gene as maternal, Sudan grass strain S722 is the male parent BC2F1 that backcrosses to get.
5. molecular markers for identification and backcrossing for the third time for the second time: the utilization method identical with step 3 identifies the individual plant that carries the bmr-6 gene and is female parent, and Sudan grass strain S722 is the male parent BC3F1 that backcrosses to get.
6. for the third time molecular markers for identification and self cross and selection: utilize the method identical with step 3 to identify the then selfing of BC3F1 plant of carrying the bmr-6 gene, the individual plant that selection has an arteries and veins phenotype in the brown in the self progeny can obtain the Sudan grass new lines of high digestibility.
Obtained the Sudan grass new lines S722-bmr6 of high digestibility by above step.Than the method for utilizing phenotypic evaluation to backcross of routine, this method has been saved one-year age.
Embodiment 2: the molecular labeling of the Chinese sorghum strain of high digestibility is auxiliary to be cultivated
1. high digestibility incross: utilize arteries and veins Chinese sorghum strain U68(bmr-6 type in the brown) with Chinese sorghum strain Tx623B hybridization.
2. backcross for the first time: take crossbreed as maternal, Chinese sorghum Tx623B is that male parent backcrosses, and obtains BC1F1.
3. offspring's individual plant molecular markers for identification: BC1F1 plants 20 strains from generation to generation, and minute individual plant extracts DNA, the individual plant that carries arteries and veins gene in the brown is carried out pcr amplification identify.
4. backcross for the second time: take the individual plant that carries the bmr-6 gene as maternal, common Chinese sorghum product are the male parent BC2F1 that backcrosses to get.
5. molecular markers for identification and backcrossing for the third time for the second time: the utilization method identical with step 3 identifies the individual plant that carries the bmr-6 gene and is female parent, and common Chinese sorghum product are the male parent BC3F1 that backcrosses to get.
6. for the third time molecular markers for identification and self cross and selection: utilize the method identical with step 3 to identify the then selfing of BC3F1 plant of carrying the bmr-6 gene, the individual plant that selection has an arteries and veins phenotype in the brown in the self progeny can obtain the New Sorghum Variety of high digestibility.
Obtained the Chinese sorghum strain Tx623B-bmr6 of high digestibility by above step.Than the method for utilizing phenotypic evaluation of routine, this method has been saved one-year age.
Claims (1)
1. the auxiliary breeding method of the molecular labeling of one kind high digestibility Sudan grass new lines is characterized in that, it comprises the steps:
1. high digestibility incross: utilize and contain the bmr-6 gene, its marker trait is the Sudan grass incross of arteries and veins in the Chinese sorghum strain of arteries and veins in the brown and the non-brown;
2. backcross for the first time: take crossbreed as maternal, the Sudan grass of arteries and veins is that male parent backcrosses in the non-brown, obtains BC1F1;
3. offspring's individual plant molecular markers for identification: at BC1F1 from generation to generation, minute individual plant extracts DNA, the individual plant that carries arteries and veins gene in the brown is carried out pcr amplification identify;
4. backcross for the second time: take the individual plant that carries the bmr-6 gene as maternal, the Sudan grass product of arteries and veins are the male parent BC2F1 that backcrosses to get in the non-brown;
5. molecular markers for identification and backcrossing for the third time for the second time: utilization and step 3. identical method identify the individual plant that carries the bmr-6 gene and are female parent, and the Sudan grass product of arteries and veins are the male parent BC3F1 that backcrosses to get in the non-brown;
6. for the third time molecular markers for identification and self cross and selection: utilize with step 3. identical method identify the then selfing of BC3F1 plant of carrying the bmr-6 gene, the individual plant that selection has an arteries and veins phenotype in the brown in the self progeny can obtain the Sudan grass new lines of high digestibility.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444518A (en) * | 2013-10-10 | 2013-12-18 | 林平 | Breeding method of high-sugar-content sorghum hybrid sudangrass variety |
| CN103960125A (en) * | 2014-05-19 | 2014-08-06 | 河北省农林科学院旱作农业研究所 | Select breeding method of new germplasm of green mowing sorghum sudanense |
| CN110679479A (en) * | 2019-11-15 | 2020-01-14 | 河北省农林科学院旱作农业研究所 | Hybrid breeding method of BMR genotype sudan grass |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898292A (en) * | 2012-08-18 | 2013-01-30 | 李杰勤 | Extraction method of sorghum brown midrib pigment cinnamic aldehyde |
| CN102893852A (en) * | 2012-08-22 | 2013-01-30 | 李杰勤 | Cultivating method of high-digestibility forage sorghum |
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2013
- 2013-07-07 CN CN2013102817008A patent/CN103299898A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898292A (en) * | 2012-08-18 | 2013-01-30 | 李杰勤 | Extraction method of sorghum brown midrib pigment cinnamic aldehyde |
| CN102893852A (en) * | 2012-08-22 | 2013-01-30 | 李杰勤 | Cultivating method of high-digestibility forage sorghum |
Non-Patent Citations (5)
| Title |
|---|
| M. D. CASLER等: "Forage Yield and Economic Losses Associated with the Brown-Midrib Trait in Sudangrass", 《CROP SCI》 * |
| 张福耀: "褐色中脉bmr高粱研究与利用进展", 《中国农业科技导报》 * |
| 李杰勤等: "高粱棕色中脉基因bmr-6的遗传分析和SSR标记定位", 《草业学报》 * |
| 詹秋文: "高粱与苏丹草的遗传及其杂种优势利用的研究", 《中国博士学位论文全文数据库 农业科技辑》 * |
| 詹秋文: "高粱苏丹草杂交种生物能源利用的前景分析", 《种子》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444518A (en) * | 2013-10-10 | 2013-12-18 | 林平 | Breeding method of high-sugar-content sorghum hybrid sudangrass variety |
| CN103960125A (en) * | 2014-05-19 | 2014-08-06 | 河北省农林科学院旱作农业研究所 | Select breeding method of new germplasm of green mowing sorghum sudanense |
| CN103960125B (en) * | 2014-05-19 | 2016-03-02 | 河北省农林科学院旱作农业研究所 | Green grass or young crops cuts the selection of class Sudan grass new germ plasm |
| CN110679479A (en) * | 2019-11-15 | 2020-01-14 | 河北省农林科学院旱作农业研究所 | Hybrid breeding method of BMR genotype sudan grass |
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Application publication date: 20130918 |