Trapping and the method for cultivating giant silkworm
Technical field
The method that the present invention relates to a kind of giant silkworm trapping and cultivate belongs to the biological technical field of cultivating.
Background technology
Silk is the symbol of Chinese mature culture, and China's ancient silk industry brilliant chapter that has been the Chinese national culture embroidery has been made indelible contribution to the development that promotes world's human civilization.China silk is well-known with the pattern and the rich culture intension of its remarkable quality, fineness.
Before several thousand, when silk passes to Europe along the silk road, it is taken, and is not only dress ornament, the ornaments that part is magnificent, the civilization of the ancient brilliance in east especially, and silk almost just becomes the disseminator and the symbol of the eastern civilization from that time.Known silk fabrics the earliest is to be unearthed in the good ruins at culture apart from modern about 4700 at present.China is the cradle of domestic silkworm silk, breeds silkworms, and filature is Ancient Times in China most important achievement on fiber utilization.As far back as the Neolithic Age; China has invented that silk is weaved and the cinnabar staining technique; After this along with the updating of loom, the improving constantly of printing technology, silk variety becomes increasingly abundant; And having formed a complete dyeing and weaving process system, the silk dyeing and weaving technological precedence that makes Ancient Times in China is in countries in the world.According to Chinese Eleventh Five-Year Plan, by 2010, cocoon yield should reach 85~900,000 tons, and silkworm raiser's annual income reaches 20,000,000,000 yuan; Silk output reaches 17~190,000 tons, and the overall water consumption power consumption in fields such as cocoon drying and silk printing and dyeing reduced by 10~20%, 250,000,000,000 yuan of silk textile industry annual value of production than 2005; 50~5,500,000,000 dollars of silk broadcloth year foreign exchange earnings, the preliminary target that realizes that " silk big country " changes to " silk power ".
In essence, silk is the main component of spun silk insect cocoon.The silk major part of using in the world wide at present is all from silkworm, i.e. our silkworm of saying usually.In addition, tusser silk also has produced in small quantities, but because its quality is not enough, usually will with fiber crops or cotton blending.The spun silk caste is a lot, in Neuroptera (Neuroptera), Trichoptera (Trichoptera), diptera (Diptera), coleoptera (Coleoptera) and Lepidoptera (Lepidoptera), the spun silk insect is arranged all.In all spun silk insects; The using value of Bombycidae in the Lepidoptera (Bombycidae) and Saturniidae (Saturniinae) (also being Saturniidae) is higher, and the oak Lasiocampidae (Lasiocampidae) that is distributed in Sicily and Greece in addition in addition has using value.Said wild silkworm mainly refers to the Saturniidae member.Saturniidae in China has: tussah belongs to, Philosamia cynthia belongs to, the willow silkworm belongs to, wild silkworm belongs to, the chestnut silkworm belongs to, passes through that the order giant silkworm belongs to, yellow order giant silkworm belongs to seven genus.In addition, Japanese rust staining giant silkworm genus, deer line giant silkworm belong to, cherish guppy giant silkworm genus, life giant silkworm genus, fourth order giant silkworm belongs to and close order giant silkworm genus etc., and be also on the books in China.Wherein have economic worth and can propagate artificially have 14 kinds at least, wherein economic worth the highest when number Io moths, amber silkworm, giant silkworm.The giant silkworm that is that wild or breed record are arranged in China at present.
Giant silkworm is called yamama again, belongs to Lepidoptera, and Saturniidae has another name called the mountain silkworm, and formal name used at school Antheraea yamamai Guerin-Meneville mainly is distributed in ground such as China, Korea, Korea S, Japan.It silk cocoon is green, can filature, and silk quality is graceful, soft, and dispense with dyeing just can keep natural green, and has special gloss, is made into beautiful in colour, attractive in appearance behind the silk, is senior silk fabric.Wild sky silk cocoon is oblong, and major diameter 45~53cm, minor axis 23~27cm, cocoon look have dark green light green or dark golden yellow; Major part is green, and a side color is darker, and a side is more shallow, and the sky silk cocoon of artificial feeding is a yellow green; Cocoon ability filature, but 1000 cocoon reel silk from cocoons raw silk 250~300g, the long 600~700m of silk, thick one times of fiber number than mulberry silk; Degree of stretching is 40~50%, and is higher 1.8 times than mulberry silk, and brute force is 2.5 times of mulberry silk; Being made into behind the silk beautiful in colourly, attractive in appearance, can mixing with other fiber and knit, is the silk fabrics of high level.In addition, giant silkworm except wild silk yarn, the larva of giant silkworm, silkworm chrysalis and to become moth all be extremely important medicinal material and health products, therefore, giant silkworm is the insect resources with higher economic worth and DEVELOPMENT PROSPECT.
The greatest problem that in development and use giant silkworm resource, runs at present is: 1) germplasm problem, 2) novel seeding technique, 3) how to use the technology of the wild giant silkworm cross-breeding of multiple different regions.Crossbreeding (bybridization) refers to hybridize between different population, different genotype individuality, and in its hybrid generation, breeds the method for the kind of isozygotying through selection.Hybridization can make parents' gene reconfigure, and forms various type, so that abundant material to be provided.
Traditional giant silkworm collection mode is that larva is collected or cocoon is picked up in the field; But because day silkworm larva is with to belong to other spun silk insect forms together very approaching, and larva and cocoon catch an illness easily, therefore; The giant silkworm disease is spread unchecked for a long time, can't realize large-scale cultivation and industrialization sustainable development.
Summary of the invention
For overcoming larva and cocoon is caught an illness easily, can't realize the problem of large-scale cultivation, the method that the present invention provides a kind of giant silkworm trapping and cultivates.
The present invention realizes through following technical proposal: a kind of trapping and the method for cultivating giant silkworm is characterized in that through the following step:
A. catching the phase giant silkworm moth, is under 100000~150000lux condition in luminous intensity, catches giant silkworm moth with trapper;
B. the male giant silkworm moth and the female giant silkworm moth that steps A are captured by the quantity ratio of the female giant silkworm moth of male Tian Can E ︰=3 ︰, 1-5 ︰ 3, are 24~27 ℃ in dark and temperature, and humidity is under 60-80% condition, matches, is fertilized;
C. spray the hormone mixed solution to the female giant silkworm moth of step B after fertilization; And be that 28-30 ℃, humidity are under 75% condition in temperature; On oopod, lay eggs; Wherein the hormone mixed solution is: by the amount of 10~30mg/10000 head, it is in 2.5% the glucose solution that moulting hormone is dissolved in 10L concentration, promptly gets the hormone mixed solution;
D. select the placement of scattering of silkworm seed that step C produced, and in 26~28 ℃ of held 10~18 days, ripe silkworm seed, and place 8~10 ℃ to preserve down;
E. with the ripe silkworm seed of step D, under super-clean environment,, dry up the silkworm seed of to sterilize again with aqua sterilisa washing 3 times, and with 25 ℃ of constant temperature with silkworm seed medicining liquid dipping 5 minutes;
F. at the sterilization silkworm seed surface sprinkling compositing acid solution of step e, it is that 28 ℃, humidity are 70~85% sericulture box that silkworm seed is placed temperature, and using illumination intensity is that the red light irradiation of 1500~2500lux is to hatching into newly-hatched silkworm;
G. the hatching newly-hatched silkworm of step F is placed the silkworm box after the sterilization; Put into the oak blade after the sterilization again, cover gauze, placing temperature then is that 26~28 ℃, humidity are that 60~75% sericulture box is cultivated; Grow to 5L until giant silkworm; Giant silkworm is moved in the box that spins, and in the box that spins, insert wooden stick, let giant silkworm spin;
H. collecting the silk cocoon of step G, use the compositing acid solution identical with step F to spray silk cocoon, is that 26~28 ℃, humidity are under 60~75% conditions in temperature, silk cocoon is sprouted wings after, repeating step C, D, E promptly obtain the strong ripe silkworm seed of resistance against diseases.
The giant silkworm moth seizure phase in the said steps A is: the Yunnan giant silkworm moth seizure phase at the beginning of annual 4 months~by the end of May and at the beginning of 9 months~mid-October; Hunan and Liaoning giant silkworm moth seizure phase be the mid-July~by the end of August.
The trapper of said steps A comprises the high-pressure sodium lamp that is arranged on the support, is arranged on the reflector of high-pressure sodium lamp one side, and the light of high-pressure sodium lamp is the simulation points light source, 360 degree irradiations.
The giant silkworm moth of said steps A catches: when night, female giant silkworm moth occurred; After entangling female giant silkworm moth with mesh bag; Female giant silkworm moth is hung on set high-pressure sodium lamp place, utilize the sex pheromone of female giant silkworm moth secretion to attract male giant silkworm moth, and entangle male giant silkworm moth with mesh bag.
The oopod of said step C comprises mesh bag, places the orifice plate of mesh bag, and female giant silkworm moth is laid eggs on orifice plate.
Selecting of silkworm seed is among the said step D: selection surface color homogeneous, the ovum that chorion is complete.
The silkworm seed thimerosal is that effective chlorine density is 0.8% liquor natrii hypochloritis in the said step e.
The compositing acid solution of said step F and H is composed of the following components: arginine 0.05~0.1g/L, cysteine 0.1~0.3g/L, methionine 0.05~0.3g/L, glycine 0.1~0.3g/L, tryptophan 0.05~0.1g/L, its pH value is 6.0~6.8.
Among the said step G; Sericulture box sterilization is: using concentration is that 1% liquor potassic permanganate and concentration are that 1% hydrogen peroxide solution is by the mixed mixed liquor of 1:1 volume ratio; The sericulture box was soaked in thimerosal 20 minutes, and the water flushing is to colourless, at last with 65 ℃ of oven dry again; The sterilization of oak leaf is: with fresh oak leaf water flush away dust; Again the oak leaf being immersed in volumetric concentration is in 70% the alcohol 30 seconds; The water flushing is 2 minutes then, in following thimerosal, soaks 10 minutes again: the solid-liquid mass ratio by 1 ︰ 400 is dissolved in the thimerosal that makes in 400 parts of water with 1 part of highly enriched sterilization effervescent tablet of chlorine dioxide; Water flushing back dries again, is used for the feeding giant silkworm; And in incubation, in time clear up the sericulture box, remove excreta, and per 14 days to the sterilization of sericulture box once.
The 5L that giant silkworm among the said step G grows among the 5L is the criterion of giant silkworm growth conditions, and corresponding to weaving silk early stage, the figure shows as the volume maximum, can see its sericterium transparence from the back.
The small wood that the preferred diameter of the box wooden stick that spins of said step G is 0.5 centimetre tilts to insert in the box that spins, and insertion depth is 4 centimetres, and the angle of inclination is 45 degree.
The giant silkworm of the said step G condition preferred illumination intensity that spins is the white light of 4500~6000lux, 26 ℃ of temperature, humidity 75%.
The present invention has advantage and beneficial effect: adopt such scheme, male giant silkworm moth that catches and female giant silkworm moth are matched, are fertilized, let female giant silkworm moth lay eggs afterwards; Let ovum hatch into newly-hatched silkworm; Cultivate newly-hatched silkworm to spinning, silk cocoon sprouted wings, lay eggs again, hatch, be cultured to spin, silk cocoon sprouts wings; So repeat, promptly obtain the strong ripe silkworm seed of resistance against diseases.Thoroughly solved the key problem of the wild giant silkworm utilization of resources, made the crossbreeding of giant silkworm become possibility.Giant silkworm hybrid seeding of the present invention and hand mating, the technology of laying eggs; Its spawning rate reaches more than 90%; The quantity of laying eggs is 200~450/female moth, and average every female giant silkworm moth quantity of laying eggs can reach 370, and eclosion rate can reach more than 95% in the silk cocoon 30 days.Giant silkworm indoor feeding method of the present invention can be realized high efficiency, high-servival rate and become the cocoon rate, promotes the giant silkworm industrialization effectively, to giant silkworm breeding, the production of hybrid seeds and large-scale farming, has great economic implications.
Description of drawings
Fig. 1 is the trapper structural representation, and among the figure, 1 is support, and 2 is high-pressure sodium lamp, and 3 is reflector.
Fig. 2 is the oopod structural representation, and among the figure, 10 is mesh bag, and 9 is porous plate.
Fig. 3 is a sericulture box structural representation, and among the figure, 5 is housing, and 4 is the collecting dung chamber, and 6 is silkworm nursery, and 7 are the gauze lid, and 8 for isolating gauze.
Fig. 4 is the box structural representation that spins, and among the figure, 11 is box body, and 12 is wooden stick, and 13 are the gauze lid.
Embodiment
To combine embodiment to describe the present invention below, but it will be understood to those of skill in the art that the following example only is used to explain the present invention, and should not be regarded as limiting scope of the present invention.
Embodiment 1
A. catch the phase giant silkworm moth, at the beginning of promptly annual 4 months (Yunnan), antenna height is 2 meters a support 1; And high-pressure sodium lamp 2 being set on support 1 top, the light of high-pressure sodium lamp 2 is the simulation points light source, 360 degree irradiations; Being provided with in high-pressure sodium lamp 2 one sides highly is 4 meters reflector 3, and the control luminous intensity is 100000lux, when night, female giant silkworm moth occurred; After entangling female giant silkworm moth with mesh bag; Female giant silkworm moth is hung on set high-pressure sodium lamp place, utilize the sex pheromone of female giant silkworm moth secretion to attract male giant silkworm moth, and entangle male giant silkworm moth with mesh bag;
B. the male giant silkworm moth and the female giant silkworm moth that steps A are captured by the quantity ratio of the female giant silkworm moth of male Tian Can E ︰=3 ︰ 1, are 24 ℃ in dark and temperature, and humidity is under 60% condition, matches, is fertilized;
C. spray the hormone mixed solution to the female giant silkworm moth of step B after fertilization; And be that 28 ℃, humidity are under 75% condition in temperature; On oopod, lay eggs; Wherein: the hormone mixed solution is the amount by the 10mg/10000 head, and it is the hormone mixed solution that obtains in 2.5% the glucose solution that moulting hormone is dissolved in 10L concentration; Oopod comprises mesh bag 10, places the orifice plate 9 of mesh bag 10, and female giant silkworm moth is laid eggs on orifice plate 9;
D. select the placement of scattering of the complete silkworm seed of surface color homogeneous, chorion that step C produced, and in 26 ℃ of held 18 days, ripe silkworm seed, place 80 ℃ to preserve down;
E. with the ripe silkworm seed of step D, under super-clean environment, use the silkworm seed thimerosal, promptly effective chlorine density is 0.8% liquor natrii hypochloritis, soaks 5 minutes, dries up again with conventional aqua sterilisa washing 3 times, and with 25 ℃ of constant temperature;
F. spray the compositing acid solution of an amount of following ingredients by routine on the sterilization silkworm seed surface of step e: arginine 0.05g/L, cysteine 0.1g/L, methionine 0.05g/L, glycine 0.3g/L, tryptophan 0.1g/L; The pH value is 6.0; It is that 28 ℃, humidity are the silkworm nursery 6 of 70% sericulture box that silkworm seed is placed temperature, use illumination intensity as the red light irradiation of 1500lux to hatching into newly-hatched silkworm;
G. the hatching newly-hatched silkworm of step F is placed the sericulture box after the sterilization; The sterilization of this sericulture box is: promptly using concentration is that 1% liquor potassic permanganate and concentration are that 1% hydrogen peroxide solution is by the mixed mixed liquor of 1:1 volume ratio; The sericulture box was soaked in thimerosal 20 minutes; The water flushing is to colourless, 65 ℃ of oven dry down again; Put into the oak blade after the sterilization afterwards; The sterilization of this oak leaf is: with fresh oak leaf water flush away dust, again the oak leaf being immersed in volumetric concentration is in 70% the alcohol 30 seconds, and the water flushing is 2 minutes then; Again promptly: by the solid-liquid mass ratio of 1 ︰ 400 at following thimerosal; 1 part of highly enriched sterilization effervescent tablet of chlorine dioxide is dissolved in the thimerosal that makes in 400 parts of water soaked 10 minutes, water flushing afterwards also dries, to be used for the feeding giant silkworm; Cover gauze lid 7, placing temperature then is that 26 ℃, humidity are that 60% sericulture box is cultivated, and the box of cleaning sericulture in time between culture period is removed the excreta in the sericulture box collecting dung chamber 4, and per 14 days to the sterilization of sericulture box once; Grow to 5L until giant silkworm, giant silkworm is moved in the box that spins, and on the gauze lid 13 at the box top that spins, insert wooden stick 12; These wooden stick 12 diameters are 0.5 centimetre; Tilt to insert in the box that spins, insertion depth is 4 centimetres, and the angle of inclination is 45 degree; Let the giant silkworm be the white light of 4500lux in intensity of illumination, temperature is that 26 ℃, humidity are to spin under 75% condition on wooden stick 12;
H. collecting the silk cocoon of step G, use the compositing acid solution identical with step F to spray silk cocoon, is that 26 ℃, humidity are under 60% condition in temperature, silk cocoon is sprouted wings after, repeating step C, D, E promptly obtain the strong ripe silkworm seed of resistance against diseases.
Embodiment 2
A. catch the phase giant silkworm moth, at the beginning of promptly annual 9 months (Yunnan), antenna height is 2 meters a support 1; And high-pressure sodium lamp 2 being set on support 1 top, the light of high-pressure sodium lamp 2 is the simulation points light source, 360 degree irradiations; Being provided with in high-pressure sodium lamp 2 one sides highly is 4 meters reflector 3, and the control luminous intensity is 150000lux, when night, female giant silkworm moth occurred; After entangling female giant silkworm moth with mesh bag; Female giant silkworm moth is hung on set high-pressure sodium lamp place, utilize the sex pheromone of female giant silkworm moth secretion to attract male giant silkworm moth, and entangle male giant silkworm moth with mesh bag;
B. the male giant silkworm moth and the female giant silkworm moth that steps A are captured by the quantity ratio of the female giant silkworm moth of male Tian Can E ︰=5 ︰ 3, are 27 ℃ in dark and temperature, and humidity is under 80% condition, matches, is fertilized;
C. spray the hormone mixed solution to the female giant silkworm moth of step B after fertilization; And be that 30 ℃, humidity are under 75% condition in temperature; On oopod, lay eggs; Wherein: the hormone mixed solution is the amount by the 30mg/10000 head, and it is the hormone mixed solution that obtains in 2.5% the glucose solution that moulting hormone is dissolved in 10L concentration; Oopod comprises mesh bag 10, places the orifice plate 9 of mesh bag 10, and female giant silkworm moth is laid eggs on orifice plate 9;
D. select the placement of scattering of the complete silkworm seed of surface color homogeneous, chorion that step C produced, and in 28 ℃ of held 10 days, ripe silkworm seed, place 10 ℃ to preserve down;
E. with the ripe silkworm seed of step D, under super-clean environment, use the silkworm seed thimerosal, promptly effective chlorine density is 0.8% liquor natrii hypochloritis, soaks 5 minutes, dries up again with conventional aqua sterilisa washing 3 times, and with 25 ℃ of constant temperature;
F. spray the compositing acid solution of an amount of following ingredients by routine on the sterilization silkworm seed surface of step e: arginine 0.1g/L, cysteine 0.3g/L, methionine 0.3g/L, glycine 0.1g/L, tryptophan 0.05g/L; The pH value is 6.8; It is that 28 ℃, humidity are the silkworm nursery 6 of 85% sericulture box that silkworm seed is placed temperature, use illumination intensity as the red light irradiation of 2500lux to hatching into newly-hatched silkworm;
G. the hatching newly-hatched silkworm of step F is placed the sericulture box after the sterilization; The sterilization of this sericulture box is: promptly using concentration is that 1% liquor potassic permanganate and concentration are that 1% hydrogen peroxide solution is by the mixed mixed liquor of 1:1 volume ratio; The sericulture box was soaked in thimerosal 20 minutes; The water flushing is to colourless, 65 ℃ of oven dry down again; Put into the oak blade after the sterilization afterwards; The sterilization of this oak leaf is: with fresh oak leaf water flush away dust, again the oak leaf being immersed in volumetric concentration is in 70% the alcohol 30 seconds, and the water flushing is 2 minutes then; Again promptly: by the solid-liquid mass ratio of 1 ︰ 400 at following thimerosal; 1 part of highly enriched sterilization effervescent tablet of chlorine dioxide is dissolved in the thimerosal that makes in 400 parts of water soaked 10 minutes, water flushing afterwards also dries, to be used for the feeding giant silkworm; Cover gauze lid 7, placing temperature then is that 28 ℃, humidity are that 75% sericulture box is cultivated, and the box of cleaning sericulture in time between culture period is removed the excreta in the sericulture box collecting dung chamber 4, and per 14 days to the sterilization of sericulture box once; Grow to 5L until giant silkworm, giant silkworm is moved in the box that spins, and on the gauze lid 13 at the box top that spins, insert wooden stick 12; These wooden stick 12 diameters are 0.5 centimetre; Tilt to insert in the box that spins, insertion depth is 4 centimetres, and the angle of inclination is 45 degree; Let the giant silkworm be the white light of 6000lux in intensity of illumination, temperature is that 26 ℃, humidity are to spin under 75% condition on wooden stick 12;
H. collecting the silk cocoon of step G, use the compositing acid solution identical with step F to spray silk cocoon, is that 28 ℃, humidity are under 75% condition in temperature, silk cocoon is sprouted wings after, repeating step C, D, E promptly obtain the strong ripe silkworm seed of resistance against diseases.