CN104304197A - Indoor culture and seed production method of actias selene hubner - Google Patents
Indoor culture and seed production method of actias selene hubner Download PDFInfo
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- CN104304197A CN104304197A CN201410486156.5A CN201410486156A CN104304197A CN 104304197 A CN104304197 A CN 104304197A CN 201410486156 A CN201410486156 A CN 201410486156A CN 104304197 A CN104304197 A CN 104304197A
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 27
- 241001522853 Actias selene Species 0.000 title abstract 7
- 241000255789 Bombyx mori Species 0.000 claims abstract description 112
- 238000000034 method Methods 0.000 claims abstract description 35
- 230000000249 desinfective effect Effects 0.000 claims abstract description 3
- 241000124033 Salix Species 0.000 claims description 72
- 230000013011 mating Effects 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 230000032683 aging Effects 0.000 claims description 8
- 230000003203 everyday effect Effects 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 230000012447 hatching Effects 0.000 claims description 6
- 244000052769 pathogen Species 0.000 claims description 6
- 230000001717 pathogenic effect Effects 0.000 claims description 6
- 238000005057 refrigeration Methods 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 4
- 230000002354 daily effect Effects 0.000 claims description 3
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 claims description 3
- 238000012797 qualification Methods 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 238000009941 weaving Methods 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 5
- 235000013601 eggs Nutrition 0.000 abstract 4
- 229920001155 polypropylene Polymers 0.000 abstract 3
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 238000012827 research and development Methods 0.000 abstract 1
- 238000009987 spinning Methods 0.000 abstract 1
- 229920003266 Leaf® Polymers 0.000 description 13
- 238000011534 incubation Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 241000255794 Bombyx mandarina Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 241000565359 Fraxinus chinensis Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000255975 Saturniidae Species 0.000 description 1
- 238000012365 batch cultivation Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses an indoor culture and seed production method of actias selene hubner. The indoor culture and seed production method comprises the following steps: putting incubated actias selene hubner into a PP (Propene Polymer) microwave box for culture, spinning and cocooning, collecting silkworm cocoons, putting the silkworm cocoons into a wooden gauze type seed production box for emergence, identifying male moths and female moths, independently putting the male moths and the female moths into the seed production box to freely mate according to the ratio of the male moths to the female moths of 1:1 or 1:2, collecting silkworm eggs after the female moths freely lay eggs, disinfecting the silkworm eggs, putting the disinfected silkworm eggs into the sterilized PP microwave box to incubate and obtaining the incubated actias selene hubner. The indoor culture and seed production method has the benefits that the purposes of low disease incidence, high incubating rate and high survival rate of the actias selene hubner in the whole culture process are realized; an important method is firstly provided for indoor batch culture of the actias selene hubner; the indoor culture and seed production method is innovative, simple and clear, is convenient to implement and lays a foundation for large-scale research and development of the actias selene hubner.
Description
Technical field
What the present invention relates to is the cultural method of a kind of wild silkworm, the in particular indoor culture producing method for seed of a kind of willow silkworm.
Background technology
Willow silkworm belongs to Lepidoptera Saturniidae, one of important Silk-producing insects and wild silkworm resource, staple food willow, the blades such as Chinese ash, its pupa is individual large and be rich in protein, and cocoon shape is comparatively large, the very thin corrosion resistant of silk, the silk reaching about 300m can be reeled out, in food, clothes, medicine and other fields, all there is good DEVELOPMENT PROSPECT.But because its resource is limited, lack again ripe cultural technique and support, both at home and abroad to the research of its each side and report all very few.Therefore the indoor production of hybrid seeds of the willow silkworm that conducts a research and cultural method both can enrich the knowledge and utility to wild silkworm resource, were again that broad scale research and the exploitation of willow silkworm from now on lays the foundation.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide the indoor culture producing method for seed of a kind of willow silkworm, to provide the indoor batch cultural technique of a kind of willow silkworm.
The present invention is achieved by the following technical solutions:
The invention provides the indoor culture producing method for seed of a kind of willow silkworm, comprise the following steps:
(1) the willow silkworm after hatching is placed in sterilized PP microwave box to cultivate, described PP microwave box covers and is provided with air-vent, wherein, the vent diameter of the PP microwave lid of one newly-hatched silkworm to two in age willow in age silkworm cultivation is 0.1 ~ 0.2mm, the vent diameter of the PP microwave lid of willow silkworm cultivation in three to five ages is 0.3 ~ 0.5mm, during cultivation, every day feeds fresh willow leaf, is chosen by sick silkworm in time and carries out sterilizing;
(2) treat that willow silkworm is to five later stages in age, whole body transfers khaki to by green gradually, and during body shrinkage, is aging silkworm, is gone in the carton of filling a large amount of withy and willow leaf by aging silkworm, is placed in ventilation and treats that it is weaved silk and cocoon;
(3) gather silk cocoon after weaving silk one week, refrigeration is reserved seed for planting or is directly used in the production of hybrid seeds;
(4) silk cocoon that silk cocoon step (3) gathered or refrigeration are reserved seed for planting is placed in the woody type production of hybrid seeds box of gauze sealing, treats that silk cocoon is sprouted wings, and qualification male and female, are placed in production of hybrid seeds box according to the ratio of sex ration 1:1 or 1:2 and let alone free mating;
After (5) 36 ~ 60 hours, take out male moth, appoint female moth Free-spawning 1 ~ 2 day, then female moth is taken out, concentrated collection silkworm seed after one week, after being disinfected by silkworm seed, then is placed in sterilized PP microwave box and treats that it is hatched, obtain the willow silkworm after hatching, continue cultivation according to the method described in step (1).
In described step (1), the length, width and height size of newly-hatched silkworm to two in age willow in an age silkworm cultivation PP microwave box is 20cm × 12cm × 6cm, one age newly-hatched silkworm cultivation density be 80 ~ 100/box, two age willow silkworm cultivation density be 50 ~ 60/box; The length, width and height size of willow silkworm cultivation in three to five ages PP microwave box is 25cm × 15cm × 10cm, three age willow silkworm cultivation density be 25 ~ 35/box, four age willow silkworm cultivation density be 15 ~ 20/box; Five age willow silkworm cultivation density be 10 ~ 15/box, with avoid density excessive cause silkworm easily ill and density be too small cause space waste.
In described step (1), the method of feeding environment being carried out to sterilizing comprises: the PP microwave box changing the ill silkworm pathogen of dye in time, cultivation table top and the every daily percent by volume of surrounding environment are the ethanol disinfection 3 ~ 5 times of 75%, to reduce the density of pathogen.
In described step (1), every day according to the willow leaf of the different tenderness of the silkworm age feeding of different willow silkworm, wherein, one age newly-hatched silkworm feeding leaf bud, two to three age young silkworm feeding tender leaf, four to five age grown silkworm feeding Lao Ye.
In described step (2), the length, width and height size of carton is 30cm × 20cm × 10cm, and the cultivation density of aging silkworm is 8 ~ 12/box.
In described step (4), according to putting 1 female moth and 1 male moth in each production of hybrid seeds box, or the mode of putting 1 female moth and 2 male moths in each production of hybrid seeds box carries out free mating, mutually disturbs and makes mating abundant, improve the success rate of mating to reduce.
In described step (4), the environment of the free mating of male and female is dark peace and quiet place, in order to avoid external interference, affects mating.
In described step (5), the method for disinfecting is: silkworm seed being placed in percent by volume is after the hypochlorous acid rinsing 1 ~ 2min of 0.4%, naturally dries.
The present invention has the following advantages compared to existing technology: the indoor culture producing method for seed that the invention provides a kind of willow silkworm, the method is selected the woody type production of hybrid seeds box of the PP microwave box of high pressure steam sterilization and gauze sealing to carry out respectively cultivating and the production of hybrid seeds, by controlling cultivation density and carrying out sterilizing to surrounding environment, strict control germ grows, achieve willow silkworm morbidity in whole breeding process few, incubation rate (can more than 90% be reached) and all high object of survival rate (can more than 80% be reached), first for the indoor batch cultivation of willow silkworm provides important method, the present invention is rich in new meaning, simple and clear, be convenient to implement, for the large-scale production of willow silkworm from now on and research lay the first stone.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
Present embodiments provide the indoor culture producing method for seed of a kind of willow silkworm, comprise the following steps:
(1) the PP microwave box willow silkworm after hatching being placed in high pressure steam sterilization cultivates, the length, width and height size of the described PP microwave box of one newly-hatched silkworm to two in age willow in age silkworm cultivation is 20cm × 12cm × 6cm, one age newly-hatched silkworm cultivation density be 80 ~ 100/box, two age willow silkworm cultivation density be 50 ~ 60/box; The length, width and height size of described PP microwave box of willow silkworm cultivation in three to five ages is 25cm × 15cm × 10cm, three age willow silkworm cultivation density be 25 ~ 35/box, four age willow silkworm cultivation density be 15 ~ 20/box; Five age willow silkworm cultivation density be 10 ~ 15/box, with avoid density excessive cause silkworm easily ill and density be too small cause space waste.
Cover at described PP microwave box and some equally distributed air-vents are set, wherein, the vent diameter of the PP microwave lid of one newly-hatched silkworm to two in age willow in age silkworm cultivation is 0.1 ~ 0.2mm, and the vent diameter of the PP microwave lid of willow silkworm cultivation in three to five ages is 0.3 ~ 0.5mm.
During cultivation, one newly-hatched silkworm in age feeding every day willow leaf leaf bud, two to three young silkworm in age feeding every day willow leaf tender leafs, four to five grown silkworm in age feeding every day willow leaf Lao Ye, in time sick silkworm is chosen and the PP microwave box of the sterilizing more renewed, cultivation table top and the every daily percent by volume of surrounding environment are the ethanol disinfection 3 ~ 5 times of 75%, at utmost to reduce the density of pathogen, prevent pathogen from growing.
(2) treat that willow silkworm is to five later stages in age, whole body transfers khaki to by green gradually, and during body shrinkage, for aging silkworm, aging silkworm is gone in the carton (long 30cm × wide 20cm × high 10cm) of filling a large amount of withy and willow leaf, be placed in ventilation to treat that it is weaved silk and cocoon, in each carton, the quantity of aging silkworm controls between 8 ~ 12.
(3) gather silk cocoon after weaving silk one week, refrigeration is reserved seed for planting or is directly used in the production of hybrid seeds.
(4) silk cocoon that silk cocoon step (3) gathered or refrigeration are reserved seed for planting is placed in the woody type production of hybrid seeds box of gauze sealing, treat that silk cocoon is sprouted wings, qualification male and female, according to putting 1 female moth and 2 male moths in each production of hybrid seeds box, or the mode of 1 female moth and 2 male moths lets alone free mating, production of hybrid seeds box black cloth is blocked simultaneously, be placed in quiet place, to provide one from the environment of external interference, improve the success rate of mating;
(5) 36 ~ 60 hours, take out male moth, appoint female moth Free-spawning 1 ~ 2 day, then female moth is taken out, concentrated collection silkworm seed after one week, silkworm seed being placed in percent by volume is after the hypochlorous acid rinsing 1 ~ 2min of 0.4%, naturally dry, the PP microwave box be placed in again described in step (1) treats that it hatches into newly-hatched silkworm, newly-hatched silkworm after hatching is brushed in the PP microwave box (20cm × 12cm × 6cm) of the high pressure steam sterilization being paved with willow leaf leaf bud in advance with the most small size banister bruss brush, is continued cultivation according to the described method of step (1).
Add up incubation rate and the survival rate of willow silkworm in whole cultivation and production of hybrid seeds process, concrete outcome is as shown in table 1 below:
The result statistics of incubation rate and survival rate is raised in table 1 willow silkworm larva indoor
As can be seen from Table 1, the incubation rate of whole cultivation and production of hybrid seeds process willow silkworm is more than 90%, total survival rate is higher than 80%, simultaneously, in whole cultivation and production of hybrid seeds process, the morbidity of willow silkworm is few, and this shows, cultivation producing method for seed of the present invention can be used in the promotion and application of the extensive indoor culture of willow silkworm.
Claims (8)
1. an indoor culture producing method for seed for willow silkworm, is characterized in that, comprise the following steps:
(1) the willow silkworm after hatching is placed in sterilized PP microwave box to cultivate, described PP microwave box covers and is provided with air-vent, wherein, the vent diameter of the PP microwave lid of one newly-hatched silkworm to two in age willow in age silkworm cultivation is 0.1 ~ 0.2mm, the vent diameter of the PP microwave lid of willow silkworm cultivation in three to five ages is 0.3 ~ 0.5mm, during cultivation, every day feeds fresh willow leaf, is chosen by sick silkworm in time and carries out sterilizing;
(2) treat that willow silkworm is to five later stages in age, whole body transfers khaki to by green gradually, and during body shrinkage, is aging silkworm, is gone in the carton of filling a large amount of withy and willow leaf by aging silkworm, is placed in ventilation and treats that it is weaved silk and cocoon;
(3) gather silk cocoon after weaving silk one week, refrigeration is reserved seed for planting or is directly used in the production of hybrid seeds;
(4) silk cocoon that silk cocoon step (3) gathered or refrigeration are reserved seed for planting is placed in the woody type production of hybrid seeds box of gauze sealing, treats that it is sprouted wings, and qualification male and female, are placed in production of hybrid seeds box according to the ratio of sex ration 1:1 or 1:2 and let alone free mating;
After (5) 36 ~ 60 hours, take out male moth, appoint female moth Free-spawning 1 ~ 2 day, then female moth is taken out, concentrated collection silkworm seed after one week, after being disinfected by silkworm seed, then is placed in sterilized PP microwave box and treats that it is hatched, obtain the willow silkworm after hatching, then continue cultivation according to the method described in step (1).
2. the indoor culture producing method for seed of a kind of willow silkworm according to claim 1, it is characterized in that, in described step (1), the length, width and height size of one newly-hatched silkworm to two in age willow in age silkworm cultivation PP microwave box is 20cm × 12cm × 6cm, one age newly-hatched silkworm cultivation density be 80 ~ 100/box, two age willow silkworm cultivation density be 50 ~ 60/box; The length, width and height size of willow silkworm cultivation in three to five ages PP microwave box is 25cm × 15cm × 10cm, three age willow silkworm cultivation density be 25 ~ 35/box, four age willow silkworm cultivation density be 15 ~ 20/box; Five age willow silkworm cultivation density be 10 ~ 15/box, with avoid density excessive cause silkworm easily ill and density be too small cause space waste.
3. the indoor culture producing method for seed of a kind of willow silkworm according to claim 1, it is characterized in that, in described step (1), the method of feeding environment being carried out to sterilizing comprises: the PP microwave box changing the ill silkworm pathogen of dye in time, cultivation table top and the every daily percent by volume of surrounding environment are the ethanol disinfection 3 ~ 5 times of 75%, to reduce the density of pathogen.
4. the indoor culture producing method for seed of a kind of willow silkworm according to claim 1, it is characterized in that, in described step (1), every day is according to the willow leaf of the different tenderness of the silkworm age feeding of different willow silkworm, wherein, one age newly-hatched silkworm feeding leaf bud, two to three age young silkworm feeding tender leaf, four to five age grown silkworm feeding Lao Ye.
5. the indoor culture producing method for seed of a kind of willow silkworm according to claim 1, is characterized in that, in described step (2), the length, width and height size of carton is 30cm × 20cm × 10cm, and the cultivation density of aging silkworm is 8 ~ 12/box.
6. the indoor culture producing method for seed of a kind of willow silkworm according to claim 1, it is characterized in that, in described step (4), according to putting 1 female moth and 1 male moth in each production of hybrid seeds box, or the mode of putting 1 female moth and 2 male moths in each production of hybrid seeds box carries out free mating.
7. the indoor culture producing method for seed of a kind of willow silkworm according to claim 1, is characterized in that, in described step (4), the environment of the free mating of male and female is dark peace and quiet place.
8. the indoor culture producing method for seed of a kind of willow silkworm according to claim 1, it is characterized in that, in described step (5), the method for disinfecting is: silkworm seed being placed in percent by volume is after the hypochlorous acid rinsing 1 ~ 2min of 0.4%, naturally dries.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410486156.5A CN104304197B (en) | 2014-09-22 | 2014-09-22 | The indoor culture producing method for seed of a kind of willow silkworm |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410486156.5A CN104304197B (en) | 2014-09-22 | 2014-09-22 | The indoor culture producing method for seed of a kind of willow silkworm |
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| CN104304197A true CN104304197A (en) | 2015-01-28 |
| CN104304197B CN104304197B (en) | 2016-04-06 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015290A (en) * | 2007-03-05 | 2007-08-15 | 苏州大学 | Indoor feeding method for wild silkworm |
| CN101036454A (en) * | 2006-12-30 | 2007-09-19 | 苏州大学 | Method of breeding one-year wild silkworm |
| CN102524197A (en) * | 2011-09-01 | 2012-07-04 | 湘潭市祂施尔生物科技有限公司 | Method for trapping and breeding giant silkworms |
| CN103503840A (en) * | 2013-09-25 | 2014-01-15 | 张跃杰 | Spring silkworm breeding process |
-
2014
- 2014-09-22 CN CN201410486156.5A patent/CN104304197B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101036454A (en) * | 2006-12-30 | 2007-09-19 | 苏州大学 | Method of breeding one-year wild silkworm |
| CN101015290A (en) * | 2007-03-05 | 2007-08-15 | 苏州大学 | Indoor feeding method for wild silkworm |
| CN102524197A (en) * | 2011-09-01 | 2012-07-04 | 湘潭市祂施尔生物科技有限公司 | Method for trapping and breeding giant silkworms |
| CN103503840A (en) * | 2013-09-25 | 2014-01-15 | 张跃杰 | Spring silkworm breeding process |
Non-Patent Citations (3)
| Title |
|---|
| 孙孝龙: "柳蚕的生物学习性与饲育方法", 《昆虫知识》 * |
| 杜占军 等: "辽宁省东部地区野生柳蚕的生物学特性及人工试养初报", 《蚕业科学》 * |
| 梁吉祥 等: "柞蚕的室内养殖", 《中国林副特产》 * |
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