CN107083272A - A kind of extracting method of granada seed oil - Google Patents
A kind of extracting method of granada seed oil Download PDFInfo
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- CN107083272A CN107083272A CN201710266899.5A CN201710266899A CN107083272A CN 107083272 A CN107083272 A CN 107083272A CN 201710266899 A CN201710266899 A CN 201710266899A CN 107083272 A CN107083272 A CN 107083272A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
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Abstract
The invention mainly relates to grease processing technology field, a kind of extracting method of granada seed oil is disclosed, including：After pomegranate seed slag is fermented through wine yeast, filtering, edible alkali solution is added into a filter residue, after filtering, complex enzyme is added into secondary filter residue, carry out after enzymolysis and ultrasound, filtering, the alcoholic solution that volume fraction is 64 ~ 66%, ultrasound filtration are added into three filter residues, the alcoholic solution that volume fraction is 97 ~ 99% is added into four filter residues again, filtered after ultrasound and concussion, all filtrates are merged, macroreticular resin is passed through after concentration and is eluted, rotary evaporation in vacuo is carried out again, obtains granada seed oil；Method is simple, makes the extraction rate reached of granada seed oil to 37.31%；Pomegranate seed slag is made up of pomegranate seed, pomegranate flower, granatum and pericarpium granati, abundant raw material, and nutrition is balanced, and pomegranate acid content reaches 86.7%, increases antibacterial, skin care and the effect for promoting gastrointestinal function of granada seed oil.
Description
Technical field
The invention mainly relates to grease processing technology field, more particularly to a kind of extracting method of granada seed oil.
Background technology
Pomegranate seed is the how unsaturated conjugated fatty acid of unique plant source, and its main component is punicic acid, content up to 70 ~
80%, " super CLA " is referred to as because the structure of punicic acid and CLA (CLA) are closest, its function
Property exceed CLA in general sense, granada seed oil is also rich in Antioxidative Factors, with very strong removing free radical and
The ability of anti-aging, human immune system can be protected, prevention of arterial atherosis reduces canceration, helps human body resistance cancer
Disease, obesity, diabetes and heart disease；General pomegranate is all directly to throw away pomegranate seed after squeezing the juice, and only seldom part is by depth
Processing, causes the very big wasting of resources；And current is all the extraction for being carried out using subcritical low-temperature extraction technology granada seed oil,
Complex operation, cost is higher, accordingly, it would be desirable to a kind of extracting method of new granada seed oil.
The content of the invention
In order to make up the defect of prior art, it is an object of the invention to provide a kind of extracting method of granada seed oil.
A kind of extracting method of granada seed oil, comprises the following steps：
（1）It is placed in food steamer, steams 8 ~ 10 minutes after pomegranate seed slag is collected, removes jerky mouthfeel, it is natural beneficial to digesting and assimilating
It is cooled to 40 ~ 50 DEG C, accesses wine yeast, in 22 ~ 24 DEG C of ferment at constant temperature 4 ~ 5 days, destroys the structure of pomegranate seed, enrich pomegranate
The fragrance and nutritional ingredient of seed oil, protect body health, and filtering obtains first-time filtrate and a filter residue；
（2）Filter residue is ground to 200 ~ 260 mesh, the mass concentration for adding 30 ~ 35 times of amounts of a filter residue weight is 0.4
~ 0.6% edible alkali solution, reduces the acid number of granada seed oil, increases palatability, makes the color and luster of granada seed oil pale red, increase food
It is intended to, Shelf-life, in 1 ~ 4 DEG C, 28 ~ 30kHz ultrasounds 20 ~ 25 minutes, filtering obtains secondary filtrate and secondary filter residue；
（3）The water of secondary 30 ~ 40 times of amounts of filter residue weight is added into secondary filter residue, is well mixed, adds complex enzyme, addition is
The 0.5 ~ 0.6% of secondary filter residue weight, in 40 ~ 42 DEG C, 51 ~ 53 revs/min stirring enzymolysis 160 ~ 180 minutes, then be placed in 2 ~ 4 DEG C,
34 ~ 36kHz ultrasounds 15 ~ 20 minutes, destroy the macromolecular structure of pomegranate seed slag, promote active ingredient to leach, and improve granada seed oil
Leaching rate, make full use of raw material, save production cost, filtering obtains three filtrates and three filter residues；
（4）Three filter residue weight, 70 ~ 80 times of amounts, the alcoholic solution that volume fraction is 64 ~ 66%, stirring are added into three filter residues
Uniformly, in 1 ~ 4 DEG C, 27 ~ 29kHz ultrasounds 20 ~ 25 minutes, the content of anthocyanin is improved, strengthens healthcare function, beauty and skin care, clearly
Except free radical, cardiovascular and cerebrovascular is protected, anti-aging, filtering obtains four filtrates and four filter residues；
（5）Four filter residue weight, 90 ~ 100 times of amounts, the alcoholic solution that volume fraction is 97 ~ 99%, stirring are added into four filter residues
Uniformly, prior to 1 ~ 4 DEG C, 27 ~ 29kHz ultrasounds 20 ~ 25 minutes, shake 30 ~ 40 minutes, add then at 1 ~ 4 DEG C, 46 ~ 48 revs/min
Low temperature concussion is carried out after the alcoholic solution of high concentration, low temperature ultrasonic, promotes granada seed oil to leach, the yield of granada seed oil is improved,
Filtering, obtains five filtrates and five filter residues；
（6）All filtrates are merged, are original 1/3 through rotary evaporation in vacuo to volume, temperature is 47 ~ 49 DEG C, rotating speed be 62 ~
64 revs/min, obtain concentrate；
（7）Concentrate is passed through in pretreated D101 macroreticular resins, adsorption flow rate is 0.6 ~ 0.8ml/ minutes, completely absorption
3 ~ 4 hours are stood after 24 ~ 26 DEG C, then eluent is passed through into D101 macroreticular resins, elution flow rate is 0.9 ~ 1.1ml/ minutes,
The purity of granada seed oil is improved, eluent is collected, obtains total eluent；
（8）Total eluent is subjected to rotary evaporation in vacuo, temperature is 45 ~ 47 DEG C, rotating speed is 67 ~ 69 revs/min, and rotary evaporation is extremely
No moisture, obtains granada seed oil；
（9）Packaging, examines, obtains finished product.
The step（1）Pomegranate seed slag, be made up of the raw material of following parts by weight：Pomegranate seed 27 ~ 29, pomegranate flower 11 ~ 13,
Granatum 4 ~ 6, pericarpium granati 4 ~ 6, abundant raw material, nutrition are balanced, raising pomegranate acid content, the antibacterial of increase granada seed oil,
Skin care and the effect for promoting gastrointestinal function.
The step（3）Complex enzyme, be made up of the raw material of following parts by weight：Cellulase 22 ~ 24, chitinase 1 serving 6 ~
18th, pectase 11 ~ 13, hemicellulase 4 ~ 6.
The step（4）And step（5）Alcoholic solution, with mass fraction for 2.2 ~ 2.4% edible alkali solution adjust pH
For 6.6 ~ 6.8.
The step（7）Eluent, be the alcoholic solution that volume fraction is 88 ~ 90%.
The extracting method of the granada seed oil, extracts the granada seed oil prepared.
The application method of the granada seed oil, available for culinary art and skin care, application method is various, without any toxic chemical
Reagent is remained, safety and Health, improves the class of granada seed oil.
It is an advantage of the invention that：A kind of extracting method for granada seed oil that the present invention is provided, method is simple, can carry out batch
Operation, saves a large amount of production costs, the extraction rate reached of granada seed oil is made after extracting repeatedly to 37.31%, hence it is evident that improve economic receive
Enter；Pomegranate seed slag is made up of pomegranate seed, pomegranate flower, granatum and pericarpium granati, abundant raw material, and nutrition is balanced, and punicic acid contains
Amount reaches 86.7%, increases antibacterial, skin care and the effect for promoting gastrointestinal function of granada seed oil；After pomegranate seed slag is steamed, life is removed
Puckery mouthfeel, beneficial to digesting and assimilating, access wine yeast is fermented, and destroys the structure of pomegranate seed, enriches the perfume (or spice) of granada seed oil
Taste and nutritional ingredient, protect body health；Edible alkali solution is added into a filter residue, the acid number of granada seed oil, increase is reduced
Palatability, makes the color and luster of granada seed oil pale red, increases appetite, Shelf-life；Complex enzyme is added into secondary filter residue and carries out enzyme
Solution, destroys the macromolecular structure of pomegranate seed slag, promotes active ingredient to leach, and improves the leaching rate of granada seed oil, makes full use of original
Material, saves production cost；The alcoholic solution of low concentration is added into three filter residues, and carries out low temperature ultrasonic, anthocyanin is improved
Content, strengthens healthcare function, and beauty and skin care removes free radical, protects cardiovascular and cerebrovascular, anti-aging；Added into four filter residues
Low temperature concussion is carried out after the alcoholic solution of high concentration, low temperature ultrasonic, promotes granada seed oil to leach, the yield of granada seed oil is improved；
All extract solutions are merged after concentration, D101 macroreticular resins is passed through and is purified, the purity of granada seed oil is reached 93.4%；Stone
It is for oral administration that pomegranate seed oil can be not only used for culinary art, can also external-use skin care, application method is various, without any toxic chemical residual,
Safety and Health, improves the class of granada seed oil.
Embodiment
Illustrate the present invention with specific embodiment below.
Embodiment 1
A kind of extracting method of granada seed oil, comprises the following steps：
（1）It is placed in food steamer, steams 8 minutes after pomegranate seed slag is collected, removes jerky mouthfeel, it is naturally cold beneficial to digesting and assimilating
But to 40 ~ 50 DEG C, wine yeast is accessed, in 22 DEG C of ferment at constant temperature 4 days, the structure of pomegranate seed is destroyed, enriches granada seed oil
Fragrance and nutritional ingredient, protect body health, and filtering obtains first-time filtrate and a filter residue；
（2）Filter residue is ground to 200 mesh, add the mass concentration of 30 times of amounts of a filter residue weight for 0.4% it is edible
Aqueous slkali, reduces the acid number of granada seed oil, increases palatability, makes the color and luster of granada seed oil pale red, increases appetite, extends shelf
Phase, in 1 DEG C, 28kHz ultrasound 20 minutes, filtering, obtain secondary filtrate and secondary filter residue；
（3）The water of secondary 30 times of amounts of filter residue weight is added into secondary filter residue, is well mixed, adds complex enzyme, addition is two
The 0.5% of secondary filter residue weight, in 40 DEG C, 51 revs/min stirring enzymolysis 160 minutes, then be placed in 2 DEG C, 34kHz ultrasound 15 minutes, mistake
Filter, obtains three filtrates and three filter residues；
（4）Three filter residue weight, 70 times of amounts, the alcoholic solution that volume fraction is 64% are added into three filter residues, is stirred, in
1 DEG C, 27kHz ultrasound 20 minutes, improve anthocyanin content, strengthen healthcare function, beauty and skin care, remove free radical, protect the heart
The cerebrovascular, anti-aging, filtering obtains four filtrates and four filter residues；
（5）Four filter residue weight, 90 times of amounts, the alcoholic solution that volume fraction is 97% are added into four filter residues, is stirred, first
In 1 DEG C, 27kHz ultrasound 20 minutes, then at 1 DEG C, 46 revs/min shake 30 minutes, add high concentration alcoholic solution, low temperature surpass
Low temperature concussion is carried out after sound, promotes granada seed oil to leach, the yield of granada seed oil is improved, filtering obtains five filtrates and five filters
Slag；
（6）All filtrates are merged, are original 1/3 through rotary evaporation in vacuo to volume, temperature is 47 DEG C, rotating speed be 62 turns/
Minute, obtain concentrate；
（7）Concentrate is passed through in pretreated D101 macroreticular resins, adsorption flow rate be 0.6ml/ minute, completely adsorb after
24 DEG C stand 3 hours, then are passed through eluent into D101 macroreticular resins, and elution flow rate is 0.9ml/ minutes, improves granada seed oil
Purity, collect eluent, obtain total eluent；
（8）Total eluent is subjected to rotary evaporation in vacuo, temperature is 45 DEG C, rotating speed is 67 revs/min, rotary evaporation is to anhydrous
Point, obtain granada seed oil；
（9）Packaging, examines, obtains finished product.
The step（1）Pomegranate seed slag, be made up of the raw material of following parts by weight：Pomegranate seed 27, pomegranate flower 11, pomegranate tree
Skin 4, pericarpium granati 4, abundant raw material, nutrition are balanced, improve pomegranate acid content, increase antibacterial, skin care and the promotion of granada seed oil
The effect of gastrointestinal function.
The step（3）Complex enzyme, be made up of the raw material of following parts by weight：Cellulase 22, chitinase 1 serving 6, pectin
Enzyme 11, hemicellulase 4.
The step（4）And step（5）Alcoholic solution, the edible alkali solution for being 2.2% with mass fraction regulation pH be
6.6~6.8。
The step（7）Eluent, be the alcoholic solution that volume fraction is 88%.
The extracting method of the granada seed oil, extracts the granada seed oil prepared.
The application method of the granada seed oil, available for culinary art and skin care, application method is various, without any toxic chemical
Reagent is remained, safety and Health, improves the class of granada seed oil.
Embodiment 2
A kind of extracting method of granada seed oil, comprises the following steps：
（1）It is placed in food steamer, steams 9 minutes after pomegranate seed slag is collected, removes jerky mouthfeel, it is naturally cold beneficial to digesting and assimilating
But to 40 ~ 50 DEG C, wine yeast is accessed, in 23 DEG C of ferment at constant temperature 4.5 days, the structure of pomegranate seed is destroyed, enriches granada seed oil
Fragrance and nutritional ingredient, protect body health, filtering, obtain first-time filtrate and a filter residue；
（2）Filter residue is ground to 240 mesh, add the mass concentration of 33 times of amounts of a filter residue weight for 0.5% it is edible
Aqueous slkali, reduces the acid number of granada seed oil, increases palatability, makes the color and luster of granada seed oil pale red, increases appetite, extends shelf
Phase, in 2 DEG C, 29kHz ultrasound 23 minutes, filtering, obtain secondary filtrate and secondary filter residue；
（3）The water of secondary 35 times of amounts of filter residue weight is added into secondary filter residue, is well mixed, adds complex enzyme, addition is two
The 0.5% of secondary filter residue weight, in 41 DEG C, 52 revs/min stirring enzymolysis 170 minutes, then be placed in 3 DEG C, 35kHz ultrasound 18 minutes, mistake
Filter, obtains three filtrates and three filter residues；
（4）Three filter residue weight, 75 times of amounts, the alcoholic solution that volume fraction is 65% are added into three filter residues, is stirred, in
2 DEG C, 28kHz ultrasound 22 minutes, improve anthocyanin content, strengthen healthcare function, beauty and skin care, remove free radical, protect the heart
The cerebrovascular, anti-aging, filtering obtains four filtrates and four filter residues；
（5）Four filter residue weight, 95 times of amounts, the alcoholic solution that volume fraction is 98% are added into four filter residues, is stirred, first
In 2 DEG C, 28kHz ultrasound 23 minutes, then at 2 DEG C, 47 revs/min shake 35 minutes, add high concentration alcoholic solution, low temperature surpass
Low temperature concussion is carried out after sound, promotes granada seed oil to leach, the yield of granada seed oil is improved, filtering obtains five filtrates and five filters
Slag；
（6）All filtrates are merged, are original 1/3 through rotary evaporation in vacuo to volume, temperature is 48 DEG C, rotating speed be 63 turns/
Minute, obtain concentrate；
（7）Concentrate is passed through in pretreated D101 macroreticular resins, adsorption flow rate be 0.7ml/ minute, completely adsorb after
25 DEG C stand 3.5 hours, then are passed through eluent into D101 macroreticular resins, and elution flow rate is 1.0ml/ minutes, improves pomegranate seed
The purity of oil, collects eluent, obtains total eluent；
（8）Total eluent is subjected to rotary evaporation in vacuo, temperature is 46 DEG C, rotating speed is 68 revs/min, rotary evaporation is to anhydrous
Point, obtain granada seed oil；
（9）Packaging, examines, obtains finished product.
The step（1）Pomegranate seed slag, be made up of the raw material of following parts by weight：Pomegranate seed 28, pomegranate flower 12, pomegranate tree
Skin 5, pericarpium granati 5, abundant raw material, nutrition are balanced, improve pomegranate acid content, increase antibacterial, skin care and the promotion of granada seed oil
The effect of gastrointestinal function.
The step（3）Complex enzyme, be made up of the raw material of following parts by weight：Cellulase 23, chitinase 1 serving 7, pectin
Enzyme 12, hemicellulase 5.
The step（4）And step（5）Alcoholic solution, the edible alkali solution for being 2.3% with mass fraction regulation pH be
6.6~6.8。
The step（7）Eluent, be the alcoholic solution that volume fraction is 89%.
The extracting method of the granada seed oil, extracts the granada seed oil prepared.
The application method of the granada seed oil, available for culinary art and skin care, application method is various, without any toxic chemical
Reagent is remained, safety and Health, improves the class of granada seed oil.
Embodiment 3
A kind of extracting method of granada seed oil, comprises the following steps：
（1）It is placed in food steamer, steams 10 minutes after pomegranate seed slag is collected, removes jerky mouthfeel, it is naturally cold beneficial to digesting and assimilating
But to 50 DEG C, wine yeast is accessed, in 24 DEG C of ferment at constant temperature 5 days, the structure of pomegranate seed is destroyed, enriches the fragrance of granada seed oil
And nutritional ingredient, body health is protected, filtering obtains first-time filtrate and a filter residue；
（2）Filter residue is ground to 260 mesh, add the mass concentration of 35 times of amounts of a filter residue weight for 0.6% it is edible
Aqueous slkali, reduces the acid number of granada seed oil, increases palatability, makes the color and luster of granada seed oil pale red, increases appetite, extends shelf
Phase, in 4 DEG C, 30kHz ultrasound 25 minutes, filtering, obtain secondary filtrate and secondary filter residue；
（3）The water of secondary 40 times of amounts of filter residue weight is added into secondary filter residue, is well mixed, adds complex enzyme, addition is two
The 0.6% of secondary filter residue weight, in 42 DEG C, 53 revs/min stirring enzymolysis 180 minutes, then be placed in 4 DEG C, 36kHz ultrasound 20 minutes, mistake
Filter, obtains three filtrates and three filter residues；
（4）Three filter residue weight, 80 times of amounts, the alcoholic solution that volume fraction is 66% are added into three filter residues, is stirred, in
4 DEG C, 29kHz ultrasound 25 minutes, improve anthocyanin content, strengthen healthcare function, beauty and skin care, remove free radical, protect the heart
The cerebrovascular, anti-aging, filtering obtains four filtrates and four filter residues；
（5）Four filter residue weight, 100 times of amounts, the alcoholic solution that volume fraction is 99% are added into four filter residues, is stirred,
Prior to 4 DEG C, 29kHz ultrasound 25 minutes, then at 4 DEG C, 48 revs/min shake 40 minutes, add high concentration alcoholic solution, low temperature
Low temperature concussion is carried out after ultrasound, promotes granada seed oil to leach, the yield of granada seed oil is improved, filtering obtains five filtrates and five times
Filter residue；
（6）All filtrates are merged, are original 1/3 through rotary evaporation in vacuo to volume, temperature is 49 DEG C, rotating speed be 64 turns/
Minute, obtain concentrate；
（7）Concentrate is passed through in pretreated D101 macroreticular resins, adsorption flow rate be 0.8ml/ minute, completely adsorb after
26 DEG C stand 4 hours, then are passed through eluent into D101 macroreticular resins, and elution flow rate is 1.1ml/ minutes, improves granada seed oil
Purity, collect eluent, obtain total eluent；
（8）Total eluent is subjected to rotary evaporation in vacuo, temperature is 47 DEG C, rotating speed is 69 revs/min, rotary evaporation is to anhydrous
Point, obtain granada seed oil；
（9）Packaging, examines, obtains finished product.
The step（1）Pomegranate seed slag, be made up of the raw material of following parts by weight：Pomegranate seed 29, pomegranate flower 13, pomegranate tree
Skin 6, pericarpium granati 6, abundant raw material, nutrition are balanced, improve pomegranate acid content, increase antibacterial, skin care and the promotion of granada seed oil
The effect of gastrointestinal function.
The step（3）Complex enzyme, be made up of the raw material of following parts by weight：Cellulase 24, chitinase 1 serving 8, pectin
Enzyme 13, hemicellulase 6.
The step（4）And step（5）Alcoholic solution, the edible alkali solution for being 2.4% with mass fraction regulation pH be
6.6~6.8。
The step（7）Eluent, be the alcoholic solution that volume fraction is 90%.
The extracting method of the granada seed oil, extracts the granada seed oil prepared.
The application method of the granada seed oil, available for culinary art and skin care, application method is various, without any toxic chemical
Reagent is remained, safety and Health, improves the class of granada seed oil.
Comparative example 1
Removal step（1）In steam, remaining method, be the same as Example 1.
Comparative example 2
Removal step（1）In wine yeast fermentation, remaining method, be the same as Example 1.
Comparative example 3
Step（2）In edible alkali solution be changed to water, remaining method, be the same as Example 1.
Comparative example 4
Removal step（3）, remaining method, be the same as Example 1.
Comparative example 5
Removal step（4）, remaining method, be the same as Example 1.
Comparative example 6
Removal step（5）, remaining method, be the same as Example 1.
Comparative example 7
Removal step（7）, remaining method, be the same as Example 1.
Comparative example 8
The supercritical CO of existing traditional granada seed oil2Extracting process.
The extraction effect of embodiment and comparative example granada seed oil extracting method：
Fresh, complete, the ripe Huaiyuan pomegranate fruit 110kg of random selection, is randomly divided into 11 groups, respectively embodiment group and right
Ratio group, every group of 10kg, embodiment group and comparative example group extract granada seed oil according to the extracting method of the group respectively, detect pomegranate
The extraction effect of the extraction effect of seed oil, embodiment and comparative example granada seed oil extracting method is shown in Table 1.
Table 1：The extraction effect of embodiment and comparative example granada seed oil extracting method
| Project | Granada seed oil recovery rate（%） | Anthocyanin recovery rate（%） | Pomegranate acid content（%） | Purity（%） |
| Embodiment 1 | 37.24 | 1.34 | 86.3 | 92.8 |
| Embodiment 2 | 37.31 | 1.53 | 86.7 | 93.4 |
| Embodiment 3 | 37.29 | 1.42 | 86.5 | 93.1 |
| Comparative example 1 | 36.57 | 1.66 | 83.3 | 92.9 |
| Comparative example 2 | 34.29 | 1.15 | 82.6 | 91.5 |
| Comparative example 3 | 33.76 | 1.07 | 82.7 | 92.6 |
| Comparative example 4 | 28.64 | 0.87 | 80.5 | 92.4 |
| Comparative example 5 | 27.83 | 0.95 | 81.3 | 93.2 |
| Comparative example 6 | 29.18 | 0.89 | 81.5 | 91.7 |
| Comparative example 7 | 35.47 | 1.05 | 83.1 | 82.4 |
| Comparative example 8 | 20.32 | 0.76 | 79.8 | 92.3 |
Show from the result of table 1, the extracting method of the granada seed oil of embodiment, granada seed oil recovery rate is substantially high compared with comparative example,
Anthocyanin recovery rate and pomegranate acid content are substantially high compared with comparative example, and purity is higher, illustrate carrying for the granada seed oil that the present invention is provided
Take method that there is good extraction effect.
Claims (7)
1. a kind of extracting method of granada seed oil, it is characterised in that comprise the following steps：
（1）It is placed in food steamer, steams 8 ~ 10 minutes after pomegranate seed slag is collected, naturally cool to 40 ~ 50 DEG C, accesses grape wine ferment
Mother, in 22 ~ 24 DEG C of ferment at constant temperature 4 ~ 5 days, filtering obtained first-time filtrate and a filter residue；
（2）Filter residue is ground to 200 ~ 260 mesh, the mass concentration for adding 30 ~ 35 times of amounts of a filter residue weight is 0.4
~ 0.6% edible alkali solution, in 1 ~ 4 DEG C, 28 ~ 30kHz ultrasounds 20 ~ 25 minutes, filtering obtains secondary filtrate and secondary filter residue；
（3）The water of secondary 30 ~ 40 times of amounts of filter residue weight is added into secondary filter residue, is well mixed, adds complex enzyme, addition is
The 0.5 ~ 0.6% of secondary filter residue weight, in 40 ~ 42 DEG C, 51 ~ 53 revs/min stirring enzymolysis 160 ~ 180 minutes, then be placed in 2 ~ 4 DEG C,
34 ~ 36kHz ultrasounds 15 ~ 20 minutes, filtering, obtain three filtrates and three filter residues；
（4）Three filter residue weight, 70 ~ 80 times of amounts, the alcoholic solution that volume fraction is 64 ~ 66%, stirring are added into three filter residues
Uniformly, in 1 ~ 4 DEG C, 27 ~ 29kHz ultrasounds 20 ~ 25 minutes, filtering obtains four filtrates and four filter residues；
（5）Four filter residue weight, 90 ~ 100 times of amounts, the alcoholic solution that volume fraction is 97 ~ 99%, stirring are added into four filter residues
Uniformly, prior to 1 ~ 4 DEG C, 27 ~ 29kHz ultrasounds 20 ~ 25 minutes, shaken 30 ~ 40 minutes then at 1 ~ 4 DEG C, 46 ~ 48 revs/min, mistake
Filter, obtains five filtrates and five filter residues；
（6）All filtrates are merged, are original 1/3 through rotary evaporation in vacuo to volume, temperature is 47 ~ 49 DEG C, rotating speed be 62 ~
64 revs/min, obtain concentrate；
（7）Concentrate is passed through in pretreated D101 macroreticular resins, adsorption flow rate is 0.6 ~ 0.8ml/ minutes, completely absorption
3 ~ 4 hours are stood after 24 ~ 26 DEG C, then eluent is passed through into D101 macroreticular resins, elution flow rate is 0.9 ~ 1.1ml/ minutes,
Eluent is collected, total eluent is obtained；
（8）Total eluent is subjected to rotary evaporation in vacuo, temperature is 45 ~ 47 DEG C, rotating speed is 67 ~ 69 revs/min, and rotary evaporation is extremely
No moisture, obtains granada seed oil；
（9）Packaging, examines, obtains finished product.
2. the extracting method of granada seed oil according to claim 1, it is characterised in that the step（1）Pomegranate seed slag, by
The raw material composition of following parts by weight：Pomegranate seed 27 ~ 29, pomegranate flower 11 ~ 13, granatum 4 ~ 6, pericarpium granati 4 ~ 6.
3. the extracting method of granada seed oil according to claim 1, it is characterised in that the step（3）Complex enzyme, by with
The raw material composition of lower parts by weight：Cellulase 22 ~ 24, chitinase 1 serving 6 ~ 18, pectase 11 ~ 13, hemicellulase 4 ~ 6.
4. the extracting method of granada seed oil according to claim 1, it is characterised in that the step（4）And step（5）Wine
Smart solution, it is 6.6 ~ 6.8 to adjust pH with the edible alkali solution that mass fraction is 2.2 ~ 2.4%.
5. the extracting method of granada seed oil according to claim 1, it is characterised in that the step（7）Eluent, be body
Fraction is 88 ~ 90% alcoholic solution.
6. according to the extracting method of any one of claim 1 ~ 5 granada seed oil, it is characterised in that extract the pomegranate seed prepared
Oil.
7. the application method of granada seed oil according to claim 6, it is characterised in that available for culinary art and skin care.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723085A (en) * | 2017-11-20 | 2018-02-23 | 五河童师傅食品有限公司 | A kind of method of tea-seed oil efficient pressing |
| WO2019056920A1 (en) * | 2017-09-25 | 2019-03-28 | 嘉必优生物技术（武汉）股份有限公司 | Microbial oil and preparation method therefor |
| CN112391223A (en) * | 2020-11-18 | 2021-02-23 | 蚌埠硕佳生物科技有限公司 | High-efficiency high-quality pomegranate seed oil extraction method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1810944A (en) * | 2005-10-06 | 2006-08-02 | 安徽乳泉石榴酒业有限公司 | Cold squeezing process of pomegranate seed oil |
| CN105886100A (en) * | 2016-05-18 | 2016-08-24 | 蚌埠市九华油脂有限公司 | Preparation method of high-quality pomegranate seed oil |
-
2017
- 2017-04-21 CN CN201710266899.5A patent/CN107083272A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1810944A (en) * | 2005-10-06 | 2006-08-02 | 安徽乳泉石榴酒业有限公司 | Cold squeezing process of pomegranate seed oil |
| CN100371430C (en) * | 2005-10-06 | 2008-02-27 | 安徽乳泉石榴酒业有限公司 | Cold squeezing process of pomegranate seed oil |
| CN105886100A (en) * | 2016-05-18 | 2016-08-24 | 蚌埠市九华油脂有限公司 | Preparation method of high-quality pomegranate seed oil |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019056920A1 (en) * | 2017-09-25 | 2019-03-28 | 嘉必优生物技术（武汉）股份有限公司 | Microbial oil and preparation method therefor |
| CN107723085A (en) * | 2017-11-20 | 2018-02-23 | 五河童师傅食品有限公司 | A kind of method of tea-seed oil efficient pressing |
| CN112391223A (en) * | 2020-11-18 | 2021-02-23 | 蚌埠硕佳生物科技有限公司 | High-efficiency high-quality pomegranate seed oil extraction method |
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Application publication date: 20170822 |