CN106520422A - Anti-aging mushroom concentrated beverage and preparation method thereof - Google Patents
Anti-aging mushroom concentrated beverage and preparation method thereof Download PDFInfo
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Abstract
The invention provides edible mushroom concentrated beverage with an auxiliary anti-aging effect and a preparation method thereof. The preparation method comprises the following steps: cleaning and crushing mushrooms such as tricholoma lobayense heim, shiitake, lucid ganoderma, agrocybe cylindracea, pleurotus ostreatus, pholiota nameko and dictyophora indusiata which serve as raw materials, and adding beer for leaching and extracting to obtain mushroom concentrate; and uniformly mixing with prepared mushroom proteins and mushroom polysaccharide, adding ingredients, and performing homogenization, sterilization, hot-canning, sealing and cooling. The concentrated beverage prepared based on the preparation method has the advantages of rich nutrition and unique flavor, and the conditions of whole technological process are mild, active ingredients of edible mushrooms can be well retained. The concentrated beverage is edible mushroom concentrated beverage with the auxiliary anti-aging effect.
Description
Technical field
The present invention relates to a kind of preparation method of beverage concentrate, and in particular to a kind of with the edible of auxiliary senile-resistant efficacy
Bacterium beverage concentrate and preparation method.
Background technology
Aging is a kind of natural phenomenon, is the inexorable law of life hit, be body degenerate period function reduction and
The general performance of physiologic derangement.Although can not resist, can delay.Edible fungi has higher edible and medical value,
Containing multivitamin, mineral and aminoacid needed by human, also containing more bioactive substance, such as polysaccharide, triterpene
Class compound etc., its senile-resistant efficacy significantly, are extensively applied in fields such as food, medicine and cosmetics.
Invention describes a kind of defying age beverage concentrate and preparation method, by the food of various food and medicament dual-purposes according to certain
Ratio mixing, make a kind of comprehensive beverage, the beverage is with Tricholoma lobayense Heim, Lentinus Edodess, Ganoderma, agrocyb eaegerita, Pleurotus ostreatus, Pholiota nameko, bamboo
Based on the edible fungi such as sweet-smelling grass, nutrition equilibrium, the significant beverage concentrate of sweet mouthfeel, anti-aging effects is obtained；Medicated beer is wherein added to make
For extracting solution, the beer acerbity of wheat fragrance and uniqueness is not only added, make concentrated solution amino is rich in having more local flavor, and medicated beer
The multiple nutritional components such as acid, mineral, yeast, improve the nutritive value of concentrated solution, promote absorbing for concentrated solution, beer
Ethanol in wine can dissolve the small-molecule active substances such as phenols in edible fungi, pigment, flavone；The mushroom added in extracting solution
Albumen and polysaccharide can occur synergism with small molecule well, promote senile-resistant efficacy；As concentrated solution in storage, transport
Can be with save space, cost-effective, although whole technical process mild condition is time-consuming longer, equipment investment is relatively costly, fits
Close and produce in enormous quantities.
At present, the concentration herbal tea Product Report being processed into by mushroom is few, and Chinese invention patent is " with consolidating for nourishing the stomach effect
Body beverage and preparation method thereof "（Number of patent application：CN201610035197.1）" a kind of antitumor, the sea of enhance immunity
Foreign book on Chinese herbal medicine compound preparation and preparation method thereof "（Number of patent application：) and " red monas rice-green tea heart protection dietetic therapy is matched somebody with somebody CN201510414763.5
Eat or for food product "（Number of patent application：) etc. CN201210311064.4 the beverage described in is not to aid in defying age as main work(
Effect；And patent of the present invention is with various mushroom as raw material, with medicated beer as extracting solution, increase unique nutrition and local flavor, mushroom albumen and
The addition of polysaccharide can promote the absorption of small-molecule substance, greatly improve the active component of edible fungi；And thick flavor is all, mouthfeel is soft
With, be it is a kind of with auxiliary senile-resistant efficacy edible fungi beverage concentrate.
The content of the invention
The present invention provides a kind of defying age beverage concentrate and preparation method, and whole production process meets 5S management systems, work
Skill process condition is gentle, can greatly retain the active component of edible fungi, its object is to the formula by improving beverage concentrate
And production technology, there is provided a kind of nutrition equilibrium, sweet mouthfeel simultaneously have significant anti-aging effects, meet people's life lengthening,
The pursuit remained youthful forever.
Foregoing invention purpose is achieved in that and employs technical scheme below：
A kind of mushroom beverage concentrate of defying age, it is characterised in that：It is made up of following raw material：Mushroom extracting solution 100-
150 parts, mushroom polysaccharide 5-10 parts, mushroom albumen 5-10 parts, 0.01 part of oligomeric isomaltose, 0.8 part of resveratrol and benzoic acid
0.001 part.
The preparation method of described mushroom extracting solution, concretely comprises the following steps：
（1）Raw material Tricholoma lobayense Heim 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pleurotus ostreatus 10-20g is weighed, it is sliding
Mushroom 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 5-10min, and water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted into 2h, ultrasonic 20-35min with the medicated beer of its 30 times of quality；
（7）Mixed liquor after ultrasound is concentrated into into the 1/3-1/2 of original volume, mushroom extracting solution is obtained final product.
The preparation method of described mushroom albumen and mushroom polysaccharide, concretely comprises the following steps：
（1）Raw material Tricholoma lobayense Heim 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pleurotus ostreatus 10-20g is weighed, it is sliding
Mushroom 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 5-10min, and water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）In the front edible alkali for processing 20-30 times of addition in mixture, ultrasound assisted extraction is carried out with ultrasonic cleaner, surpassed
The sound time is 20-35min, and ultrasonic temperature is 45-55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The precipitate that centrifugation is obtained, adjusts pH to albumen isoelectric point, IP with citric acid, and after precipitation is redissolved, tune pH is into
Property, mushroom albumen is obtained after being dried.
The preparation method of described mushroom polysaccharide, concretely comprises the following steps
（1）Raw material Tricholoma lobayense Heim 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pleurotus ostreatus 10-20g is weighed, it is sliding
Mushroom 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 5-10min, and water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）Edible alkali being added in front process mixture, ultrasound assisted extraction being carried out with ultrasonic cleaner, ultrasonic time is
20-35min, ultrasonic temperature are 45-55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The 1/3-1/2 of the supernatant concentration to original volume that centrifugation is obtained；
（9）By step（8）The concentrated solution being concentrated to give carries out 4 DEG C of 3 times of volume dehydrated alcohol overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）The precipitate that centrifugation is obtained redissolves, and is dried, obtains final product mushroom polysaccharide.
It is 20-30 with the mass ratio of pre-treatment mixture that the liquid ratio of described edible alkali is edible alkali:1
A kind of method of the mushroom beverage concentrate for preparing described defying age, comprises the following steps that：
（1）Raw material is weighed by formula proportion；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With the multi-functional concentrator that extracts by step（2）Mixed material is concentrated into original volume 1/3-1/2, makes concentrated solution；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 120-140 DEG C, and the time is 4-6s；
（5）It is canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
Beneficial effects of the present invention：
（1）The wheat that medicated beer can be increased using medicated beer is fragrant, make concentrated solution have more in local flavor and medicated beer rich in aminoacid, mineral,
The multiple nutritional components such as yeast, improve the nutritive value of concentrated solution, promote absorbing for concentrated solution, the ethanol in medicated beer
The small-molecule active substances such as phenols in edible fungi, pigment, flavone can be dissolved；
（2）Polysaccharide, albumen and small molecule active composition in edible fungi has the feature of defying age to human body；Mushroom albumen and many
Adding for sugar can occur synergism with the small-molecule substance in medicated beer extracting solution, and the addition of resveratrol can make activity of fighting against senium
Greatly enhance；
（3）Whole technical conditions are gentle, greatly remain the active component of edible fungi, and make concentrated solution and be more beneficial for
Preserve, save space is cost-effective, it is convenient to transport for long-distance, be it is a kind of with auxiliary senile-resistant efficacy and convenient storage concentration
Beverage.
Specific embodiment：
For fully open defying age beverage concentrate of the present invention and preparation method thereof, illustrate with reference to example.
Embodiment 1
A kind of mushroom beverage concentrate of defying age, it is characterised in that：It is made up of following raw material：100 parts of mushroom extracting solution,
0.001 part of 5 parts of mushroom polysaccharide, 5 parts of mushroom albumen, 0.01 part of oligomeric isomaltose, 0.8 part of resveratrol and benzoic acid
The preparation method of described mushroom extracting solution, concretely comprises the following steps：
（1）Weigh raw material Tricholoma lobayense Heim 10g, Lentinus Edodess 10g, Ganoderma 10g, agrocyb eaegerita 10g, Pleurotus ostreatus 10g, Pholiota nameko 10g, Caulis Bambusae In Taeniam 10g and
Propolis 5g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 5min, and water temperature is 25 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 2h, and temperature is 35 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted into 2h, ultrasonic 20min with the medicated beer of its 30 times of quality；
（7）Mixed liquor after ultrasound is concentrated into into the 1/3 of original volume, mushroom extracting solution is obtained final product.
The preparation method of described mushroom albumen and mushroom polysaccharide, concretely comprises the following steps：
（1）Weigh raw material Tricholoma lobayense Heim 10g, Lentinus Edodess 10g, Ganoderma 10g, agrocyb eaegerita 10g, Pleurotus ostreatus 10g, Pholiota nameko 10g, Caulis Bambusae In Taeniam 10g and
Propolis 5g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 5min, and water temperature is 25 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 2h, and temperature is 35 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）In the front edible alkali for processing 20 times of quality of addition in mixture, ultrasound assisted extraction is carried out with ultrasonic cleaner, surpassed
The sound time is 20min, and ultrasonic temperature is 45 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The precipitate that centrifugation is obtained, adjusts pH to albumen isoelectric point, IP with citric acid, and after precipitation is redissolved, tune pH is into
Property, mushroom albumen is obtained after being dried.
The preparation method of described mushroom polysaccharide, concretely comprises the following steps
（1）Weigh raw material Tricholoma lobayense Heim 10g, Lentinus Edodess 10g, Ganoderma 10g, agrocyb eaegerita 10g, Pleurotus ostreatus 10g, Pholiota nameko 10g, Caulis Bambusae In Taeniam 10g and
Propolis 5g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 5min, and water temperature is 25 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 2h, and temperature is 35 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）In the front edible alkali for processing 20 times of quality of addition in mixture, ultrasound assisted extraction is carried out with ultrasonic cleaner, surpassed
The sound time is 20min, and ultrasonic temperature is 45 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The supernatant concentration that obtains of centrifugation is to the 1/3 of original volume；
（9）By step（8）The concentrated solution being concentrated to give carries out 4 DEG C of 3 times of volume dehydrated alcohol overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）The precipitate that centrifugation is obtained redissolves, and is dried, obtains final product mushroom polysaccharide.
A kind of method of the mushroom beverage concentrate for preparing described defying age, comprises the following steps that：
（1）Raw material is weighed by formula proportion；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With the multi-functional concentrator that extracts by step（2）Mixed material is concentrated into original volume 1/3, makes concentrated solution；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 120 DEG C, and the time is 4s；
（5）It is canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
Embodiment 2
A kind of mushroom beverage concentrate of defying age, it is characterised in that：It is made up of following raw material：120 parts of mushroom extracting solution,
0.001 part of 7 parts of mushroom polysaccharide, 7 parts of mushroom albumen, 0.01 part of oligomeric isomaltose, 0.8 part of resveratrol and benzoic acid.
The preparation method of described mushroom extracting solution, concretely comprises the following steps：
（1）Weigh raw material Tricholoma lobayense Heim 15g, Lentinus Edodess 15g, Ganoderma 15g, agrocyb eaegerita 15g, Pleurotus ostreatus 15g, Pholiota nameko 15g, Caulis Bambusae In Taeniam 15g and
Propolis 8g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 8min, and water temperature is 28 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 3h, and temperature is 40 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted into 2h, ultrasonic 30min with the medicated beer of its 30 times of quality；
（7）Mixed liquor after ultrasound is concentrated into into the 1/3 of original volume, mushroom extracting solution is obtained final product.
The preparation method of described mushroom albumen, concretely comprises the following steps：
（1）Weigh raw material Tricholoma lobayense Heim 15g, Lentinus Edodess 15g, Ganoderma 15g, agrocyb eaegerita 15g, Pleurotus ostreatus 15g, Pholiota nameko 15g, Caulis Bambusae In Taeniam 15g and
Propolis 8g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 8min, and water temperature is 28 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 3h, and temperature is 40 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）In the front edible alkali for processing 25 times of quality of addition in mixture, ultrasound assisted extraction is carried out with ultrasonic cleaner, surpassed
The sound time is 28min, and ultrasonic temperature is 50 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The precipitate that centrifugation is obtained, adjusts pH to albumen isoelectric point, IP with citric acid, and after precipitation is redissolved, tune pH is into
Property, mushroom albumen is obtained after being dried.
The preparation method of described mushroom polysaccharide, concretely comprises the following steps
（1）Weigh raw material Tricholoma lobayense Heim 15g, Lentinus Edodess 15g, Ganoderma 15g, agrocyb eaegerita 15g, Pleurotus ostreatus 15g, Pholiota nameko 15g, Caulis Bambusae In Taeniam 15g and
Propolis 8g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 8min, and water temperature is 28 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 3h, and temperature is 40 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）In the front edible alkali for processing 25 times of quality of addition in mixture, ultrasound assisted extraction is carried out with ultrasonic cleaner, surpassed
The sound time is 30min, and ultrasonic temperature is 50 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The supernatant concentration that obtains of centrifugation is to the 1/3 of original volume；
（9）By step（8）The concentrated solution being concentrated to give carries out 4 DEG C of 3 times of volume dehydrated alcohol overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）The precipitate that centrifugation is obtained redissolves, and is dried, obtains final product mushroom polysaccharide.
A kind of method of the mushroom beverage concentrate for preparing described defying age, comprises the following steps that：
（1）Raw material is weighed by formula proportion；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With the multi-functional concentrator that extracts by step（2）Mixed material is concentrated into original volume 1/3, makes concentrated solution；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 130 DEG C, and the time is 5s；
（5）It is canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
Embodiment 3
A kind of mushroom beverage concentrate of defying age, it is characterised in that：It is made up of following raw material：150 parts of mushroom extracting solution,
0.001 part of 10 parts of mushroom polysaccharide, mushroom protein 10 part, 0.01 part of oligomeric isomaltose, 0.8 part of resveratrol and benzoic acid.
The preparation method of described mushroom extracting solution, concretely comprises the following steps：
（1）Weigh raw material Tricholoma lobayense Heim 20g, Lentinus Edodess 20g, Ganoderma 20g, agrocyb eaegerita 20g, Pleurotus ostreatus 20g, Pholiota nameko 20g, Caulis Bambusae In Taeniam 20g and
Propolis 10g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 10min, and water temperature is 30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 4h, and temperature is 45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted into 2h, ultrasonic 35min with the medicated beer of its 30 times of quality；
（7）Mixed liquor after ultrasound is concentrated into into the 1/2 of original volume, mushroom extracting solution is obtained final product.
The preparation method of described mushroom albumen, concretely comprises the following steps：
（1）Weigh raw material Tricholoma lobayense Heim 20g, Lentinus Edodess 20g, Ganoderma 20g, agrocyb eaegerita 20g, Pleurotus ostreatus 20g, Pholiota nameko 20g, Caulis Bambusae In Taeniam 20g and
Propolis 10g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 10min, and water temperature is 30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 4h, and temperature is 45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）In the front edible alkali for processing 30 times of addition in mixture, ultrasound assisted extraction is carried out with ultrasonic cleaner, when ultrasonic
Between be 35min, ultrasonic temperature be 55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The precipitate that centrifugation is obtained, adjusts pH to albumen isoelectric point, IP with citric acid, and after precipitation is redissolved, tune pH is into
Property, mushroom albumen is obtained after being dried.
The preparation method of described mushroom polysaccharide, concretely comprises the following steps
（1）Weigh raw material Tricholoma lobayense Heim 20g, Lentinus Edodess 20g, Ganoderma 20g, agrocyb eaegerita 20g, Pleurotus ostreatus 20g, Pholiota nameko 20g, Caulis Bambusae In Taeniam 20g and
Propolis 10g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 10min, and water temperature is 30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 4h, and temperature is 45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）In the front edible alkali for processing 30 times of quality of addition in mixture, ultrasound assisted extraction is carried out with ultrasonic cleaner, surpassed
The sound time is 35min, and ultrasonic temperature is 55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The supernatant concentration that obtains of centrifugation is to the 1/2 of original volume；
（9）By step（8）The concentrated solution being concentrated to give carries out 4 DEG C of 3 times of volume dehydrated alcohol overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）The precipitate that centrifugation is obtained redissolves, and is dried, obtains final product mushroom polysaccharide.
A kind of method of the mushroom beverage concentrate for preparing described defying age, comprises the following steps that：
（1）Raw material is weighed by formula proportion；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With the multi-functional concentrator that extracts by step（2）Mixed material is concentrated into original volume 1/2, makes concentrated solution；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 140 DEG C, and the time is 6s；
（5）It is canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
Research anti-aging effects of the mushroom beverage concentrate to Male Drosophila, test with the black abdomen Male Drosophila of wild type as animal
2d age Male Drosophilas are randomly divided into 2 groups by model, 200 per group, i.e. matched group and administration group, time recording fruit bat death toll
Mesh draws the average longevity of administration group fruit bat counting the average life and maximum life span of fruit bat by drawing life span of drosophila melanogaster curve chart
Life and maximum life span significantly extend compared with matched group, it follows that mushroom beverage concentrate has the work(of preferably auxiliary defying age
Effect.
Quality index detection is carried out to beverage concentrate using the method for sampling survey.Sampling Detection, sample is under natural light
Observation, color and luster are light yellow；Fructus Hordei Germinatus with the distinctive fragrance of edible fungi and medicated beer are fragrant, without other abnormal flavour；Mouthfeel is smooth, tart flavour
It is soft pure；Clarification, without precipitation, no suspended substance；Physical and chemical index is total acid（With citrometer）>4.5g/100mL, reducing sugar（With
Glucose meter）>1.5g/100mL, lead 2.0mg/L；Sanitary index be miscellaneous bacteria index 500CFU/mL, escherichia coli
0.43CFU/ml, mycete and yeast 100CFU/ml, other bacterium must not be detected.
Profile of the table 1 for embodiment 1, the performance of endoplasm quality
Table 2 is 1 physics and chemistry hygienic quality situation of embodiment
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with repair
Decorations, should all belong to the covering scope of the present invention.
Claims (7)
1. the mushroom beverage concentrate of a kind of defying age, it is characterised in that：It is made up of following raw material：Mushroom extracting solution 100-
150 parts, mushroom polysaccharide 5-10 parts, mushroom albumen 5-10 parts, 0.01 part of oligomeric isomaltose, 0.8 part of resveratrol and benzoic acid
0.001 part.
2. the mushroom beverage concentrate of defying age according to claim 1, it is characterised in that：The system of described mushroom extracting solution
Preparation Method, concretely comprises the following steps：
Weigh raw material Tricholoma lobayense Heim 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pleurotus ostreatus 10-20g, Pholiota nameko
10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 5-10min, and water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted into 2h, ultrasonic 20-35min with the medicated beer of its 30 times of quality；
（7）Mixed liquor after ultrasound is concentrated into into the 1/3-1/2 of original volume, mushroom extracting solution is obtained final product.
3. the mushroom beverage concentrate of defying age according to claim 1, it is characterised in that：The preparation of described mushroom albumen
Method, concretely comprises the following steps：
（1）Raw material Tricholoma lobayense Heim 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pleurotus ostreatus 10-20g is weighed, it is sliding
Mushroom 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 5-10min, and water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）Edible alkali being added in front process mixture, ultrasound assisted extraction being carried out with ultrasonic cleaner, ultrasonic time is
20-35min, ultrasonic temperature are 45-55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The precipitate that centrifugation is obtained, adjusts pH to albumen isoelectric point, IP with acid, after precipitation is redissolved, adjusts pH to neutrality,
Mushroom albumen is obtained after drying.
4. the mushroom beverage concentrate of defying age according to claim 3, it is characterised in that：Described acid is citric acid.
5. the mushroom beverage concentrate of defying age according to claim 1, it is characterised in that：The preparation of described mushroom polysaccharide
Method, concretely comprises the following steps：
（1）Raw material Tricholoma lobayense Heim 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pleurotus ostreatus 10-20g is weighed, it is sliding
Mushroom 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material is respectively washed with Spray-cleaning Machine, scavenging period is 5-10min, and water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven drying, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is crushed respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder of gained mixes, and obtains pre-treatment mixture；
（6）In the front edible alkali for processing 20-30 times of addition in mixture, ultrasound assisted extraction is carried out with ultrasonic cleaner, surpassed
The sound time is 20-35min, and ultrasonic temperature is 45-55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, 20min is centrifuged under 8000rpm；
（8）By step（7）The 1/3-1/2 of the supernatant concentration to original volume that centrifugation is obtained；
（9）By step（8）4 DEG C of 3 times of volume dehydrated alcohol of the concentrated solution being concentrated to give overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）The precipitate that centrifugation is obtained redissolves, and is dried, obtains final product mushroom polysaccharide.
6. the mushroom beverage concentrate of the defying age according to claim 3 or 5, it is characterised in that：Described edible alkali with it is front
The mass ratio for processing mixture is 20-30:1.
7. a kind of method of the mushroom beverage concentrate for preparing defying age as claimed in claim 1, it is characterised in that：
Comprise the following steps that：
（1）Raw material is weighed by formula proportion；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With the multi-functional concentrator that extracts by step（2）Mixed material is concentrated into original volume 1/3-1/2, makes concentrated solution；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 120-140 DEG C, and the time is 4-6s；
（5）It is canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
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| CN109043495A (en) * | 2018-07-25 | 2018-12-21 | 辽宁省农业科学院 | A kind of preparation method of edible fungi root extract |
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