CN106472934A - A kind of mushroom beverage concentrate of enhance immunity and preparation method - Google Patents
A kind of mushroom beverage concentrate of enhance immunity and preparation method Download PDFInfo
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- CN106472934A CN106472934A CN201610875997.4A CN201610875997A CN106472934A CN 106472934 A CN106472934 A CN 106472934A CN 201610875997 A CN201610875997 A CN 201610875997A CN 106472934 A CN106472934 A CN 106472934A
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 74
- 230000036039 immunity Effects 0.000 title claims abstract description 39
- 235000008504 concentrate Nutrition 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000000203 mixture Substances 0.000 claims abstract description 45
- 241000221377 Auricularia Species 0.000 claims abstract description 17
- 241000222336 Ganoderma Species 0.000 claims abstract description 17
- 240000000599 Lentinula edodes Species 0.000 claims abstract description 17
- 235000001715 Lentinula edodes Nutrition 0.000 claims abstract description 17
- 244000168667 Pholiota nameko Species 0.000 claims abstract description 17
- 235000014528 Pholiota nameko Nutrition 0.000 claims abstract description 17
- 235000013405 beer Nutrition 0.000 claims abstract description 17
- 150000004676 glycans Chemical class 0.000 claims abstract description 17
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 17
- 239000005017 polysaccharide Substances 0.000 claims abstract description 17
- 238000004140 cleaning Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000000605 extraction Methods 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims description 66
- 238000002203 pretreatment Methods 0.000 claims description 32
- 239000000463 material Substances 0.000 claims description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 29
- 239000002244 precipitate Substances 0.000 claims description 21
- 238000001035 drying Methods 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 16
- 241000241413 Propolis Species 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 15
- 229940069949 propolis Drugs 0.000 claims description 15
- 230000002000 scavenging effect Effects 0.000 claims description 15
- 238000001291 vacuum drying Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000003513 alkali Substances 0.000 claims description 12
- 229960004756 ethanol Drugs 0.000 claims description 12
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 10
- 239000012467 final product Substances 0.000 claims description 10
- 238000002137 ultrasound extraction Methods 0.000 claims description 10
- 235000013361 beverage Nutrition 0.000 claims description 9
- -1 compound vitamin Chemical class 0.000 claims description 7
- 238000005070 sampling Methods 0.000 claims description 7
- 239000011782 vitamin Substances 0.000 claims description 7
- 235000013343 vitamin Nutrition 0.000 claims description 7
- 229940088594 vitamin Drugs 0.000 claims description 7
- 229930003231 vitamin Natural products 0.000 claims description 7
- 239000005711 Benzoic acid Substances 0.000 claims description 5
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 claims description 5
- 235000010233 benzoic acid Nutrition 0.000 claims description 5
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 5
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 claims description 5
- 238000002372 labelling Methods 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 239000011669 selenium Substances 0.000 claims 1
- 229910052711 selenium Inorganic materials 0.000 claims 1
- 239000011691 vitamin B1 Substances 0.000 claims 1
- 239000011701 zinc Substances 0.000 claims 1
- 229910052725 zinc Inorganic materials 0.000 claims 1
- 241000233866 Fungi Species 0.000 abstract description 14
- 240000001080 Grifola frondosa Species 0.000 abstract description 10
- 235000007710 Grifola frondosa Nutrition 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 9
- 239000000796 flavoring agent Substances 0.000 abstract description 6
- 235000019634 flavors Nutrition 0.000 abstract description 6
- 238000009924 canning Methods 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 235000008935 nutritious Nutrition 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 12
- 150000003384 small molecules Chemical class 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000012041 food component Nutrition 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 235000015092 herbal tea Nutrition 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940088592 immunologic factor Drugs 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- 235000019590 thick flavour Nutrition 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Non-Alcoholic Beverages (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention proposes a kind of edible fungi beverage concentrate with auxiliary enhance immunity effect and preparation method, and its preparation method comprises the following steps：With mushroom such as Ganoderma, Lentinus Edodess, agrocyb eaegerita, Auricularia, Caulis Bambusae In Taeniam, Pholiota nameko, Grifola frondosa as major ingredient, after cleaning and crush, add medicated beer extraction, prepared mushroom concentrated liquid；And mix with prepared mushroom albumen and mushroom polysaccharide, add dispensing, homogenizing, sterilization, hot canning, sealed cans, cooling.The beverage concentrate obtaining produced according to the present invention is nutritious, unique flavor, and entirely technical conditions are gentle, can be effectively maintained the active component of edible fungi, is a kind of edible fungi beverage concentrate with auxiliary enhance immunity effect.
Description
Technical field
The present invention relates to a kind of preparation method of beverage concentrate is and in particular to a kind of have auxiliary enhance immunity effect
Edible fungi beverage concentrate and preparation method.
Background technology
Immunity is the defense mechanism of human body itself, is that human bioequivalence is (viral, thin with any foreign body eliminating external intrusion
Bacterium etc.), process aging, damage, the dead, own cells of degeneration, and identification and process vivo mutations cell and viral infection
The ability of cell, immunity is also that the mankind adapt to environment for a long time, in order to seek the opposing that survival and development are formed in body interior
Disease, is divided into specific immunity and nonspecific immunity, not only relevant with the immunogene of human body itself and immune factor etc., also with
Posteriori living habit is closely related with environment.Therefore, improve the joint demand that immunity is people.
Invention describes a kind of enhance immunity beverage concentrate and preparation method, by the food of multiple food and medicament dual-purposes according to
Certain ratio mixing, makes a kind of comprehensive beverage, this beverage is with Auricularia, Lentinus Edodess, Ganoderma, agrocyb eaegerita, Pholiota nameko, ash tree
Based on the edible fungi such as flower, Caulis Bambusae In Taeniam, it is obtained balanced in nutrition, sweet mouthfeel, enhance immunity act on significant beverage concentrate；Wherein plus
Enter medicated beer as extracting solution, not only add wheat fragrance and unique beer acerbity, make concentrated solution have more local flavor, and in medicated beer
Rich in multiple nutritional components such as aminoacid, mineral, yeast, improve the nutritive value of concentrated solution, promote the suction of concentrated solution
Receive and utilize, the ethanol in medicated beer can dissolve the small-molecule active substances such as phenols in edible fungi, pigment, albumen；In extracting solution
Well and there is synergism in small molecule for the mushroom albumen adding and polysaccharide energy, promotes enhance immunity effect；As concentration
Liquid can be with save space, cost-effective, although whole technical process mild condition takes longer, equipment in storage, transport
Input cost is higher, is suitable for producing in enormous quantities.
At present, the concentration herbal tea Product Report being processed into by mushroom is few, and " one kind has antitumor and exempts from Chinese invention patent
Composition of multiple fungus of epidemic disease adjustment effect and preparation method thereof and purposes "（Number of patent application：CN200610131969.8）" a kind of
The preparation method of edible fungus nutritional health-care functional drink "（Number of patent application：) and " human body can be strengthened CN201110291447.5
Immunity, health food of resisting fatigue and preparation method thereof "（Number of patent application：) etc. CN201310593906.4 the beverage described in
It is all that Multiple components are combined, but preparation technology is simpler；And patent of the present invention is with various mushroom as raw material, with medicated beer for extracting
Liquid, increases unique nutrition and local flavor, and the addition of mushroom albumen and polysaccharide can promote the absorption of small-molecule substance, greatly improves food
Active component with bacterium；And thick flavor is all, mouthfeel is soft, is that a kind of edible fungi with auxiliary enhance immunity effect concentrates drink
Material.
Content of the invention
The present invention provides a kind of enhance immunity beverage concentrate and preparation method, and whole production process meets 5S management body
System, technical conditions are gentle, can greatly retain the active component of edible fungi, its object is to by improving beverage concentrate
Formula and production technology, provide a kind of balanced in nutrition, sweet mouthfeel and have significant enhance immunity effect, meet people
Life lengthening, the pursuit remained youthful forever.
A kind of mushroom beverage concentrate of enhance immunity, is prepared from the following raw materials in parts by weight：Mushroom extracting solution 100-150
Part, mushroom polysaccharide 5-10 part, mushroom albumen 5-10 part, 0.02 part of oligomeric isomaltose, 0.01 part of compound vitamin and benzoic acid
0.001 part.
The preparation method of described mushroom extracting solution, concretely comprises the following steps：
（1）Weigh raw material Auricularia 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pholiota nameko 10-20g, ash
Tree flower 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 5-10min, water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted 2h, ultrasonic 20-35min with the medicated beer of its 30 times of quality；
（7）Ultrasonic rear mixed liquor is concentrated into the 1/3-1/2 of original volume, obtains final product mushroom extracting solution.
The preparation method of described mushroom albumen, concretely comprises the following steps：
（1）Weigh raw material Auricularia 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pholiota nameko 10-20g, ash
Tree flower 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 5-10min, water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added edible alkali, carries out ultrasound assisted extraction with ultrasonic cleaner, ultrasonic time is 20-
35min, ultrasonic temperature is 45-55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）Be centrifuged the precipitate that obtains, adjust pH to albumen isoelectric point, IP with citric acid, after precipitation is redissolved, adjust pH in
Property, obtain mushroom albumen after being dried.
The preparation method of described mushroom polysaccharide, concretely comprises the following steps：
（1）Weigh raw material Auricularia 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pholiota nameko 10-20g, ash
Tree flower 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 5-10min, water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added the edible alkali of 20-30 times of quality, carries out ultrasound assisted extraction with ultrasonic cleaner,
Ultrasonic time is 20-35min, and ultrasonic temperature is 45-55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）It is centrifuged the supernatant concentration that obtains to the 1/3-1/2 of original volume；
（9）By step（8）The concentrated solution being concentrated to give 4 DEG C of 3 times of volume dehydrated alcohol overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）It is centrifuged the precipitate obtaining to redissolve, be dried, obtain final product mushroom polysaccharide.
Described edible alkali is 20-30 with the mass ratio of pre-treatment mixture:1.
A kind of method of the mushroom beverage concentrate preparing described enhance immunity, comprises the following steps that：
（1）Weigh each material by formula proportion respectively；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With multi-functional extraction concentrator by step（2）Mixed material carries out being concentrated into the 1/3-1/2 of original volume, makes dense
Contracting liquid；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 120-140 DEG C, and the time is 4-6s；
（5）Canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
Beneficial effects of the present invention：
（1）The wheat that medicated beer can be increased using medicated beer is fragrant, make concentrated solution have more in local flavor and medicated beer rich in aminoacid, mineral,
The multiple nutritional components such as yeast, improve the nutritive value of concentrated solution, promote absorbing of concentrated solution, the ethanol in medicated beer
The small-molecule active substances such as phenols in edible fungi, pigment, flavone can be dissolved.；
（2）Polysaccharide in edible fungi, albumen and small molecule active composition have the feature of enhance immunity to human body；Mushroom albumen
Addition with polysaccharide can occur synergism with the small-molecule substance in medicated beer extracting solution, and the addition of compound vitamin can make immunity
Power activity greatly enhances；
（3）Whole technical conditions are gentle, greatly remain the active component of edible fungi, and make concentrated solution and be more beneficial for
Preserve, save space, cost-effective, convenient transport for long-distance, be a kind of to there is auxiliary enhance immunity effect and convenient store
Beverage concentrate.
Specific embodiment
For fully disclosing enhance immunity beverage concentrate of the present invention and preparation method thereof, illustrate with reference to example.
Embodiment 1
A kind of mushroom beverage concentrate of enhance immunity, is prepared from the following raw materials in parts by weight：100 parts of mushroom extracting solution, mushroom is many
5 parts of sugar, 5 parts of mushroom albumen, 0.02 part of oligomeric isomaltose, 0.01 part of compound vitamin and 0.001 part of benzoic acid.
The preparation method of described mushroom extracting solution, concretely comprises the following steps：
（1）Weigh raw material Auricularia 10g, Lentinus Edodess 10g, Ganoderma 10g, agrocyb eaegerita 10g, Pholiota nameko 10g, Grifola frondosa 10g, Caulis Bambusae In Taeniam 10g
With propolis 5g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 5min, water temperature is 25 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 2h, and temperature is 35 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted 2h, ultrasonic 20min with the medicated beer of its 30 times of quality；
（7）Ultrasonic rear mixed liquor is concentrated into the 1/3 of original volume, obtains final product mushroom extracting solution.
The preparation method of described mushroom albumen, concretely comprises the following steps：
（1）Weigh raw material Auricularia 10g, Lentinus Edodess 10g, Ganoderma 10g, agrocyb eaegerita 10g, Pholiota nameko 10g, Grifola frondosa 10g, Caulis Bambusae In Taeniam 10g
With propolis 5g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 5min, water temperature is 25 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 2h, and temperature is 35 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added the edible alkali of 20 times of quality, carry out ultrasound assisted extraction with ultrasonic cleaner, ultrasonic
Time is 20min, and ultrasonic temperature is 45 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）Be centrifuged the precipitate that obtains, adjust pH to albumen isoelectric point, IP with citric acid, after precipitation is redissolved, adjust pH in
Property, obtain mushroom albumen after being dried.
The preparation method of described mushroom polysaccharide, concretely comprises the following steps：
（1）Weigh raw material Auricularia 10g, Lentinus Edodess 10g, Ganoderma 10g, agrocyb eaegerita 10g, Pholiota nameko 10g, Grifola frondosa 10g, Caulis Bambusae In Taeniam 10g
With propolis 5g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 5min, water temperature is 25 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 2h, and temperature is 35 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added the edible alkali of 20 times of quality, carry out ultrasound assisted extraction with ultrasonic cleaner, ultrasonic
Time is 20min, and ultrasonic temperature is 45 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）It is centrifuged the supernatant concentration that obtains to the 1/3 of original volume；
（9）By step（8）The concentrated solution being concentrated to give carries out 4 DEG C of 3 times of volume dehydrated alcohol overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）It is centrifuged the precipitate obtaining to redissolve, be dried, obtain final product mushroom polysaccharide.
A kind of method of the mushroom beverage concentrate preparing described enhance immunity, comprises the following steps that：
（1）Weigh each material by formula proportion respectively；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With multi-functional extraction concentrator by step（2）Mixed material carries out being concentrated into the 1/3 of original volume, makes concentrated solution；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 120- DEG C, and the time is 4s；
（5）Canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
Embodiment 2
A kind of mushroom beverage concentrate of enhance immunity, is prepared from the following raw materials in parts by weight：120 parts of mushroom extracting solution, mushroom is many
7 parts of sugar, 7 parts of mushroom albumen, 0.02 part of oligomeric isomaltose, 0.01 part of compound vitamin and 0.001 part of benzoic acid.
The preparation method of described mushroom extracting solution, concretely comprises the following steps：
（1）Weigh raw material Auricularia 15g, Lentinus Edodess 15g, Ganoderma 15g, agrocyb eaegerita 15g, Pholiota nameko 15g, Grifola frondosa 15g, Caulis Bambusae In Taeniam 15g
With propolis 8g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 8min, water temperature is 28 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 3h, and temperature is 40 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted 2h, ultrasonic 28min with the medicated beer of its 30 times of quality；
（7）Ultrasonic rear mixed liquor is concentrated into the 1/3 of original volume, obtains final product mushroom extracting solution.
The preparation method of described mushroom albumen, concretely comprises the following steps：
（1）Weigh raw material Auricularia 15g, Lentinus Edodess 15g, Ganoderma 15g, agrocyb eaegerita 15g, Pholiota nameko 15g, Grifola frondosa 15g, Caulis Bambusae In Taeniam 15g
With propolis 8g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 8min, water temperature is 28 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 3h, and temperature is 40 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added the edible alkali of 25 times of quality, carry out ultrasound assisted extraction with ultrasonic cleaner, ultrasonic
Time is 28min, and ultrasonic temperature is 50 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）Be centrifuged the precipitate that obtains, adjust pH to albumen isoelectric point, IP with citric acid, after precipitation is redissolved, adjust pH in
Property, obtain mushroom albumen after being dried.
The preparation method of described mushroom polysaccharide, concretely comprises the following steps：
（1）Weigh raw material Auricularia 15g, Lentinus Edodess 15g, Ganoderma 15g, agrocyb eaegerita 15g, Pholiota nameko 15g, Grifola frondosa 15g, Caulis Bambusae In Taeniam 15g
With propolis 8g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 8min, water temperature is 28 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 3h, and temperature is 40 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added the edible alkali of 25 times of quality, carry out ultrasound assisted extraction with ultrasonic cleaner, ultrasonic
Time is 28min, and ultrasonic temperature is 50 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）It is centrifuged the supernatant concentration that obtains to the 1/3 of original volume；
（9）By step（8）The concentrated solution being concentrated to give carries out 4 DEG C of 3 times of volume dehydrated alcohol overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）It is centrifuged the precipitate obtaining to redissolve, be dried, obtain final product mushroom polysaccharide.
A kind of method of the mushroom beverage concentrate preparing described enhance immunity, comprises the following steps that：
（1）Weigh each material by formula proportion respectively；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With multi-functional extraction concentrator by step（2）Mixed material carries out being concentrated into the 1/3 of original volume, makes concentrated solution；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 130 DEG C, and the time is 5s；
（5）Canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
Embodiment 3
A kind of mushroom beverage concentrate of enhance immunity, is prepared from the following raw materials in parts by weight：150 parts of mushroom extracting solution, mushroom is many
10 parts of sugar, mushroom protein 10 part, 0.02 part of oligomeric isomaltose, 0.01 part of compound vitamin and 0.001 part of benzoic acid.
The preparation method of described mushroom extracting solution, concretely comprises the following steps：
（1）Weigh raw material Auricularia 20g, Lentinus Edodess 20g, Ganoderma 20g, agrocyb eaegerita 20g, Pholiota nameko 20g, Grifola frondosa 20g, Caulis Bambusae In Taeniam 20g
With propolis 10g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 10min, water temperature is 30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 4h, and temperature is 45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted 2h, ultrasonic 35min with the medicated beer of its 30 times of quality；
（7）Ultrasonic rear mixed liquor is concentrated into the 1/2 of original volume, obtains final product mushroom extracting solution.
The preparation method of described mushroom albumen, concretely comprises the following steps：
（1）Weigh raw material Auricularia 20g, Lentinus Edodess 20g, Ganoderma 20g, agrocyb eaegerita 20g, Pholiota nameko 20g, Grifola frondosa 20g, Caulis Bambusae In Taeniam 20g
With propolis 10g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 10min, water temperature is 30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 4h, and temperature is 45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added edible alkali, carries out ultrasound assisted extraction with ultrasonic cleaner, ultrasonic time is
35min, ultrasonic temperature is 55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）Be centrifuged the precipitate that obtains, adjust pH to albumen isoelectric point, IP with citric acid, after precipitation is redissolved, adjust pH in
Property, obtain mushroom albumen after being dried.
The preparation method of described mushroom polysaccharide, concretely comprises the following steps：
（1）Weigh raw material Auricularia 20g, Lentinus Edodess 20g, Ganoderma 20g, agrocyb eaegerita 20g, Pholiota nameko 20g, Grifola frondosa 20g, Caulis Bambusae In Taeniam 20g
With propolis 10g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 10min, water temperature is 30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 4h, and temperature is 45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added the edible alkali of 30 times of quality, carry out ultrasound assisted extraction with ultrasonic cleaner, ultrasonic
Time is 35min, and ultrasonic temperature is 55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）It is centrifuged the supernatant concentration that obtains to the 1/3 of original volume；
（9）By step（8）The concentrated solution being concentrated to give carries out 4 DEG C of 3 times of volume dehydrated alcohol overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）It is centrifuged the precipitate obtaining to redissolve, be dried, obtain final product mushroom polysaccharide.
A kind of method of the mushroom beverage concentrate preparing described enhance immunity, comprises the following steps that：
（1）Weigh each material by formula proportion respectively；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With multi-functional extraction concentrator by step（2）Mixed material carries out being concentrated into the 1/2 of original volume, makes concentrated solution；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 140 DEG C, and the time is 6s；
（5）Canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
The research impact to white mice immunity for the mushroom beverage concentrate.By close to 100 20 ages in days, quality, health status
Good white mice is randomly divided into 2 groups, i.e. matched group and administration group, carries out the detection of every immune indexes after 30 days, and research is sent out
Existing, the white mice immunoglobulin of administration group, immunocyte and liver index substantially increase compared with matched group, and experiment shows, mushroom is dense
Contracting beverage has functions that preferably to assist enhance immunity.
Method using sampling survey carries out quality index detection to beverage concentrate.Sampling Detection, sample is under natural light
Observe, color and luster is light yellow；There is the distinctive fragrance of edible fungi and the Fructus Hordei Germinatus of medicated beer are fragrant, no other abnormal flavour；Mouthfeel is smooth, tart flavour
Soft pure；Clarification, no precipitates, no suspended substance；Physical and chemical index is total acid（With citrometer）>4.5g/100mL, reducing sugar（With
Glucose meter）>1.5g/100mL, lead 2.0mg/L；Sanitary index is miscellaneous bacteria index 500CFU/mL, escherichia coli
0.43CFU/ml, mycete and yeast 100CFU/ml, other bacterium must not detect.
Table 1 is the profile of embodiment 1, and endoplasm quality shows
Table 2 is embodiment 1 physics and chemistry hygienic quality situation
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with repair
Decorations, all should belong to the covering scope of the present invention.
Claims (8)
1. a kind of enhance immunity mushroom beverage concentrate it is characterised in that：It is prepared from the following raw materials in parts by weight：Mushroom extracting solution
100-150 part, mushroom polysaccharide 5-10 part, mushroom albumen 5-10 part, 0.02 part of oligomeric isomaltose, 0.01 part of compound vitamin and
0.001 part of benzoic acid.
2. enhance immunity according to claim 1 mushroom beverage concentrate it is characterised in that：Described mushroom extracting solution
Preparation method, concretely comprise the following steps：
（1）Weigh raw material Auricularia 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pholiota nameko 10-20g, ash
Tree flower 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 5-10min, water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is extracted 2h, ultrasonic 20-35min with the medicated beer of its 30 times of quality；
（7）Ultrasonic rear mixed liquor is concentrated into the 1/3-1/2 of original volume, obtains final product mushroom extracting solution.
3. enhance immunity according to claim 1 mushroom beverage concentrate it is characterised in that：Described mushroom albumen
Preparation method, concretely comprises the following steps：
（1）Weigh raw material Auricularia 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pholiota nameko 10-20g, ash
Tree flower 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 5-10min, water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added edible alkali, carries out ultrasound assisted extraction with ultrasonic cleaner, ultrasonic time is 20-
35min, ultrasonic temperature is 45-55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）It is centrifuged the precipitate obtaining, with acid tune pH to albumen isoelectric point, IP, after precipitation is redissolved, adjust pH to neutrality,
Mushroom albumen is obtained after drying.
4. enhance immunity according to claim 3 mushroom beverage concentrate it is characterised in that：Described acid is Fructus Citri Limoniae
Acid.
5. enhance immunity according to claim 1 mushroom beverage concentrate it is characterised in that：Described mushroom polysaccharide
Preparation method, concretely comprises the following steps：
（1）Weigh raw material Auricularia 10-20g, Lentinus Edodess 10-20g, Ganoderma 10-20g, agrocyb eaegerita 10-20g, Pholiota nameko 10-20g, ash
Tree flower 10-20g, Caulis Bambusae In Taeniam 10-20g and propolis 5-10g；
（2）Raw material Spray-cleaning Machine is respectively washed, scavenging period is 5-10min, water temperature is 25-30 DEG C；
（3）By step（2）Raw material is respectively placed in vacuum drying oven and dries, and the time is 2-4h, and temperature is 35-45 DEG C；
（4）With Universalpulverizer to step（3）Raw material after drying is pulverized respectively, crosses 30 mesh sieves, stand-by；
（5）By step（4）Each material powder mixing of gained, obtains pre-treatment mixture；
（6）Pre-treatment mixture is added edible alkali, carries out ultrasound assisted extraction with ultrasonic cleaner, ultrasonic time is 20-
35min, ultrasonic temperature is 45-55 DEG C；
（7）By step（6）Mixture be placed in refrigerated centrifuger, under 8000rpm be centrifuged 20min；
（8）By step（7）It is centrifuged the supernatant concentration that obtains to the 1/3-1/2 of original volume；
（9）By step（8）The concentrated solution being concentrated to give carries out 4 DEG C of 3 times of volume dehydrated alcohol overnight precipitate with ethanol；
（10）By step（9）The liquid of precipitate with ethanol is according to step（7）Method be centrifuged；
（11）By step（10）It is centrifuged the precipitate obtaining to redissolve, be dried, obtain final product mushroom polysaccharide.
6. the enhance immunity according to claim 3 or 5 mushroom beverage concentrate it is characterised in that：Described edible alkali
Mass ratio with pre-treatment mixture is 20-30:1.
7. a kind of method of the mushroom beverage concentrate preparing enhance immunity as claimed in claim 1 it is characterised in that：Specifically
Step is as follows：
（1）Weigh each material by formula proportion respectively；
（2）With high pressure homogenizer by step（1）Material is sufficiently mixed 1.5h；
（3）With multi-functional extraction concentrator by step（2）Mixed material carries out being concentrated into the 1/3-1/2 of original volume, makes dense
Contracting liquid；
（4）With beverage tubular sterilization machine by step（3）Concentrated solution is sterilized, and temperature is 120-140 DEG C, and the time is 4-6s；
（5）Canned under sterile vacuum environment, it is cooled to room temperature, labeling, vanning, sampling observation.
8. according to claim 7 preparation enhance immunity mushroom beverage concentrate method it is characterised in that：Described
Compound vitamin include：Vitamin B10.03 μ g/kg, B20.004 μ g/kg, zinc 0.2mg/kg, calcium 0.05 μ g/kg, selenium
0.1ng/kg.
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| CN109393451A (en) * | 2017-08-18 | 2019-03-01 | 青岛瑞思德生物科技有限公司 | A kind of health food that immunity can be improved containing Enteromorpha |
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| CN1176748A (en) * | 1997-05-17 | 1998-03-25 | 严良荣 | Process for extracting polysaccharide and polyphenol mixed crystal from fresh crude and old tea leaves |
| CN105360859A (en) * | 2015-11-23 | 2016-03-02 | 王健夫 | Method for processing termitomyces albuminosus milk beverage |
| CN105901697A (en) * | 2016-04-15 | 2016-08-31 | 邵素英 | Fungal extract probiotic functional food |
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|---|---|---|---|---|
| CN1176748A (en) * | 1997-05-17 | 1998-03-25 | 严良荣 | Process for extracting polysaccharide and polyphenol mixed crystal from fresh crude and old tea leaves |
| CN105360859A (en) * | 2015-11-23 | 2016-03-02 | 王健夫 | Method for processing termitomyces albuminosus milk beverage |
| CN105901697A (en) * | 2016-04-15 | 2016-08-31 | 邵素英 | Fungal extract probiotic functional food |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109393451A (en) * | 2017-08-18 | 2019-03-01 | 青岛瑞思德生物科技有限公司 | A kind of health food that immunity can be improved containing Enteromorpha |
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