CN101401567A - Eremophyte steeping specimen and method for making protective color - Google Patents
Eremophyte steeping specimen and method for making protective color Download PDFInfo
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Abstract
The invention discloses a method for manufacturing desert plant immersing specimen and color retention thereof. The method comprises the following steps of selecting materials and collecting, pruning and cleaning, immersing and preparing stationary liquid, storing the immersed stationary liquid, as well as bottling, sealing and labeling. The immersing steps of color retaining stationary liquid and a storing liquid aim to color systems of green flowers and red flowers, and are based on the characteristic that different contents of color elements are contained in plant organ positions. Continuous adjustment to chemical medicine properties, and color showing series screening for the stationary liquid and the storing liquid reasonably control acid base balance degree, and ensure that structures of color element molecules are stable; and specimen subjected to color retention treatment can clearly show morphosis of plants, and the prepared plant immersed specimen has bright color and luster, strong third dimension and lifelike form, can completely show fine morphological differences of flowers, fruit and leaves of the plants, and enrich the researches on specimen taxology and preserving method. The method uses chemical liquid medicine to immerse the specimen and retain color for unique desert plant in Xinjiang, adopts glass ceramic utensil, takes chemical agent as a common agent, and has low cost, strong operability and good market prospect.
Description
Technical field
The present invention relates to the making of herbarium,, be particularly suitable for the preparation method of eremophytes dipping specimen and guarantor's look especially at the making of dipping specimen and guarantor's look.
Background technology
The preservation of herbarium has important practical value to phytological research and means of taxonomic research.Classic methods has two kinds: the one, and pressing, be plant complete stool or branch wounded in the battle, the band fruit, be layered on the blotting paper and set level, step up, place the ventilation natural air drying with clamping plate, this method can be preserved sample in a large number, but also there are many problems,, easily make the phyllomorphosis of plant flowers and fruits as in the absorbent drying process, color change causes the closely-related information of plant and form to be lost and mislead; From the angle of botanical system classification, the integrality of plant relevant information identifies to taxonomy and just seems particularly important that the kind of some plant and the difference of kind just are the trickle morphological differences of flowers and fruits leaf of plant.The 2nd, with FAA fixer preservation method, because the effect of formaldehyde, glacial acetic acid, the long-term meeting polymerization reaction take place of placing, produce the white depositions of paraformaldehyde, make fixer become muddy, can not preserve original color and luster of herbarium, the herbarium of collection at present makes its complete, no mouldy, variable color, goes bad is a stubborn problem.
About the plant dipping specimen and protect look, fresh-keeping preparation method, disclosed the Chinese medicine dipping specimen during " polychrome is killed livestock fixation to keeping the experimental study of the former plant color and luster of Chinese medicine " of looking into new finding " Henan College Of Traditional Chinese Medicine journal " the 4th phase comrade Liu Ruoyong July in 2003 write articles and protected the experiment of look, but with the present invention at the plant object different different because the small imbalance of anthocyan soda acid in the plant corpus can make pattern change.Disclosed application experiment during in addition " plant magazine " comrade Bian Yong in 1996 " some of dipping herbarium known from experience " write articles to guarantor's look of fruit in the vegetable melon and fruit, but still with the present invention at the plant object different different.The present invention is directed to the peculiar eremophytes in Xinjiang, utilize the method for chemical liquid humid preparation, carried out the maceration extract proportioning test,, obtained best keeping color agent prescription by contrast, screening, herbarium through protecting the look processing can clearly show the form structure of plant corpus, the plant humid preparation lovely luster of making, third dimension is strong, and form is true to nature, the trickle morphological differences of flowers and fruits leaf of displaying plant that can be intact has been enriched the Study on Diversity of sample taxonomy and method for preserving.
Summary of the invention
The objective of the invention is to: the preparation method that the eremophytes dipping specimen is provided, particularly at the distinctive eremophytes of Xinjiang region, carried out the dipping experiment, observed maceration extract, be intended to obtain the scientific formula that dipping specimen is made the dipping of sample and the research of long preservation.
Another object of the present invention is: the prescription of indication can make dipping specimen keep primary colors and image appearance, and under minute eka-ytterbium clear in structure, kytoplasm, karyon are painted good, the color ratio degree is suitable, clear, has expanded eremophytes sample means of taxonomic research.
Eremophytes dipping specimen of the present invention and the preparation method of protecting look, the making of dipping specimen comprises: selection collection → pruning cleaning → fixer infuse → preservation liquid infuse → go into bottle to seal labelled step; It protects color fixing liquid:
Pink colour system flower fixer infuse: adopt in the mixed liquor of 1.8-4ml concentration 40% formalin, 2-4g boric acid, concentration 20% alcohol 8-12ml and 800-1200ml water, soak 22-26h; When non-pigment or impurity are separated out, move in the preservation liquid of 20-40ml concentration 6% sulfurous acid, 18-22g boric acid and 900-1100ml water, treat long preservation;
Green leaf, little design of scattered small flowers and plants series fixer infuse: adopt concentration 5% copper-bath, sample is soaked, this look section when presenting green → brown → yellow → green, the set time is 3-6d, shift out flush away copper sulphate, with concentration 2%～3% sulfurous acid bleaching 1-2d; When non-pigment or impurity are separated out, move in the preservation liquid of sulfurous acid, 38-42ml formaldehyde, 38-42ml glycerine, 900-1100ml water of 8-12ml concentration 6%, treat long preservation;
Red flower is the fixer infuse: adopt in the mixed liquor by 3-5ml concentration 40% formalin, 2-4g boric acid and 380-420ml water, soak 7-10d; When non-pigment or impurity are separated out, move in the preservation liquid of 18-22ml concentration 10% sulfurous acid, 8-12g boric acid and 900-1100ml water, treat long preservation;
Yellow is the fixer infuse: adopt in the formulated fixer of 38-42ml concentration 6% sulfurous acid and water 800-1000ml, soak 4-7d; When non-pigment or impurity are separated out, move in the preservation liquid of 23-27ml formalin, 23-27ml glycerine and 900-1100ml water, treat long preservation;
Peony, purple flower are the fixer infuse: adopt the magnesium chloride solution of concentration 20% to soak 2-5d, soak successively with the magnesium chloride solution of concentration 30%, 50%, 75% respectively then, soak 4-8d at every turn, control PH is 7.5-9; Soak with the magnesium chloride solution of concentration 85% respectively again, soak 6-10d at every turn after, in oversaturated magnesium chloride fixer, keep primary colors; When non-pigment or impurity are separated out, move in the preservation liquid of magnesium chloride solution of concentration 20%, treat long preservation.
The dipping specimen in last step and the preparation method of protecting look, excellent good fixer with preservation liquid infusion step is:
The pink flower is the fixer infuse: adopt in the mixed liquor of 2-3ml concentration 40% formalin, 2-3g boric acid, concentration 20% alcohol 9-11ml and 900-1100ml water, soak 23-25h; When non-pigment or impurity are separated out, move in the preservation liquid of 25-35ml concentration 6% sulfurous acid, 19-21g boric acid and 950-1050ml water, treat long preservation;
Green leaf, little design of scattered small flowers and plants series fixer infuse: adopt concentration 5% copper-bath, sample is soaked, this look section when presenting green → brown → yellow → green, the set time is 3-5d, shift out flush away copper sulphate, with concentration 2%-3% sulfurous acid bleaching 1-1.5d; When non-pigment or impurity are separated out, move in the preservation liquid of sulfurous acid, 39-41ml formaldehyde, 39-41ml glycerine, 950-1050ml water of 9-11ml concentration 6%, treat long preservation;
Red flower is the fixer infuse: adopt in the mixed liquor by 3-5ml concentration 40% formalin, 2-4g boric acid and 380-420ml water, soak 7-9d; When non-pigment or impurity are separated out, move in the preservation liquid of 19-21ml concentration 10% sulfurous acid, 9-11g boric acid and 950-1050ml water, treat long preservation;
Yellow is the fixer infuse: adopt in the formulated fixer of 39-41ml concentration 6% sulfurous acid and water 850-950ml, soak 4-6d; When non-pigment or impurity are separated out, move in the preservation liquid of 24-26ml formalin, 24-26ml glycerine and 950-1050ml water, treat long preservation;
Peony, purple flower are the fixer infuse: adopt the magnesium chloride solution of concentration 20% to soak 2-4d, soak successively with the magnesium chloride solution of concentration 30%, 50%, 75% respectively then, soak 5-7d at every turn, control PH is 7.8-8.5; Soak with the magnesium chloride solution of concentration 85% respectively again, soak 7-9d at every turn after, in oversaturated magnesium chloride fixer, keep primary colors; When non-pigment or impurity are separated out, move in the preservation liquid of magnesium chloride solution of concentration 20%, treat long preservation.
The dipping specimen in last step and the preparation method of protecting look, optimal fixation liquid with preservation liquid infusion step is:
The pink flower is the fixer infuse: adopt in the mixed liquor of 2ml concentration 40% formalin, 3g boric acid, concentration 20% alcohol 10ml and 1000ml water, soak 24h; When non-pigment or impurity are separated out, move in the preservation liquid of 30ml concentration 6% sulfurous acid, 20g boric acid and 1000ml water, treat long preservation;
Green leaf, little design of scattered small flowers and plants series fixer infuse: adopt concentration 5% copper-bath, sample is soaked, until the look section that presents green → brown → yellow → green, the set time is 4d, shifts out flush away copper sulphate, with concentration 2%～3% sulfurous acid bleaching 1d; When non-pigment or impurity are separated out, move in the preservation liquid of sulfurous acid, 40ml formaldehyde, 40ml glycerine, 1000ml water of 10ml concentration 6%, treat long preservation;
Red flower is the fixer infuse: adopt in the mixed liquor by 4ml concentration 40% formalin, 3g boric acid and 400ml water, soak 8d; When non-pigment or impurity are separated out, move in the preservation liquid of 20ml concentration 10% sulfurous acid, 10g boric acid and 1000ml water, treat long preservation;
Yellow is the fixer infuse: adopt in the formulated fixer of 40ml concentration 6% sulfurous acid and water 900ml, soak 5d; When non-pigment or impurity are separated out, move in the preservation liquid of 25ml formalin, 25ml glycerine and 1000ml water, treat long preservation;
Peony, purple flower are the fixer infuse: adopt the magnesium chloride solution of concentration 20% to soak 3d, soak successively with the magnesium chloride solution of concentration 30%, 50%, 75% respectively then, soak 6d at every turn, controlling PH is 8; Soak with the magnesium chloride solution of concentration 85% respectively again, soak 8d at every turn after, in oversaturated magnesium chloride fixer, keep primary colors; When non-pigment or impurity are separated out, move in the preservation liquid of magnesium chloride solution of concentration 20%, treat long preservation.
The preparation method of described dipping specimen, choosing is adopted sample and is got the feature typical case, the fresh anosis pityriasis simplex of branch, flower or fruit, branch length is controlled at 10-20cm, and the openness of flower is preferably 7-8, and fruit is sharp very, full.
The preparation method of described dipping specimen, the branch of pruning, flower, fruit can't harm, and cleaning is removed dust, and plant submergence section is 1.8-2.5cm, uses the glass bar shaping, and loam cake seals, and lucifuge is deposited.
The preparation method of described dipping specimen, the sample clear water rinsing that infuse is good, is immersed in and preserves in the liquid fixedly moulding, and paraffin sealing pastes tag along sort.
Described dipping specimen and the preparation method of protecting look, the fixer of dipping specimen recovers dip time 3-7 days of plant primary colors, meat, berry plant 60-90 days; Preserve and to use for a long time after liquid was changed in 1 year.
Described dipping specimen and the preparation method of protecting look, the container that sample infuse and fixation are deposited is glassware or pottery.
Research experiment mechanism of the present invention shows: the color of plant is to be determined by pigment, and the main component that presents chlorophyll, flavonoids and the carotenoid of pigment is an anthocyan, because the compound mode difference of pigment monoid, it is also different to present color, be exactly similar pigment, the difference on the amount also can cause the difference of color and luster.
Chlorophyll molecule structural formula such as figure below: the porphyrin ring compound is that four pyrrole rings and four=CH-form a big ring, conjugated double bond forms a big π key, there are a magnesium atom in porphyrin ring and ring central authorities, decision chlorophyll color, therefore when the dipping green plants, select for use certain density copper-bath to carry out substitution reaction, with the Mg in the porphyrin ring of green plants
2+By Cu
2++Replace, by Cu
2+After the replacement, it is green that plant still keeps, and through substitution reaction, makes the core texture that has copper atom in the chlorophyll molecule, and the green color relation that present in this moment can not be dissolved in organic solutions such as formaldehyde or alcohol, has stability, thereby reach the green and fresh-keeping state of long-term guarantor.
It is flower and mellow fruit that plant corpus presents red position, mainly is carotenoid and flavonoids, and flavonoids is water-soluble cytochrome, wherein mainly is anthocyan, and basic structure is that two aromatic rings (A ring and B ring) are formed structural formula such as right figure:
B ring hydroxyl increases, and purple is increased, and shows as pink, redness when containing a hydroxyl, shows as aubergine when containing two hydroxyls, shows as bluish violet when containing three hydroxyls; Hydroxyl is during by methyl oxidation, and color transforms to red aspect again; Anthocyan also often is connected with glycan molecule in spending, and forms glucosides, and it can make pattern become rich and gaudy, distinct; Therefore, in experimental formula, fix anthocyan B ring hydroxyl, and reasonably control the acid base equilibrium of solution, reach and protect look, fresh-keeping purpose with the concentration of formalin, magnesium chloride solution.
Preparation method of the present invention shows: in the dipping specimen of eremophytes is made, colour system at green and red two Da Hua, adopt scientific formula, through protecting color fixing liquid and preserving the liquid screening, make the structure of pigment molecular in the plant become stable, reach the optimum that plant corpus presents primary colors, research mechanism and experimental design have embodied the reasonability and the novelty of inventive concept, show the progress of science and technology.
Embodiment
Below in conjunction with embodiment the present invention is further described.
The preparation method of eremophytes dipping specimen of the present invention and guarantor's look as follows:
Embodiment 1
The infuse method of the green staggered flowering plum sample of powder:
Plucking openness is that 7-8 becomes, and length is the flowering plum branch of 10-20cm, prunes arrangement, makes branch, leaf, spends scattered causing, and washing branch dust gets final product infuse, for overcoming the branch come-up, ties up pressure with weight, submergence section 2-3cm; With 2ml concentration 40% formalin, 3g boric acid, 10ml concentration 20% alcohol and 1000ml water mixed stationary liquid fixation, the time is 24h; After fixation is stable, when non-pigment or impurity are separated out, take out and clean, move into 30ml concentration 6% sulfurous acid, in the preservation liquid of 20g boric acid and 1000ml water; Paraffin envelope bottleneck is labelled; Preservation liquid to be replaced gets final product long preservation.
Embodiment 2
The infuse method of the sample of yellowish green staggered saussurea involucrata:
The harvesting openness is about 8 one-tenth a lanatechead saussurea herb with flower, and plant height 10-20cm contains yellow bract of 5-6 sheet and 4-5 sheet greenery; Prune the arrangement branches and leaves, the earth of flushing root gets final product infuse, for overcoming the branch come-up, ties up on root submergence section 2-3cm with weight; Be placed on refrigerator 0-5 ℃, treat fixation; With 5% copper-bath fixer fixation, until presenting green → brown → yellow → green look section, this process is 4-5d; Shift out flush away copper sulphate, with 2%～3% sulfurous acid bleaching 1d; After fixation is stable, when non-pigment or impurity are separated out, take out and clean, immerse in the preservation liquid of 10ml 6% sulfurous acid, 40ml formaldehyde, glycerine 40ml and 1000ml water, paraffin envelope bottleneck is labelled; Preservation liquid to be replaced gets final product long preservation.
Can also flood Radix Calligoni Mongolici, sacsaoul and Chinese ephedra plant etc. with the method.
Embodiment 3
Open the infuse method of the Chinese rose sample of rose flower:
Plucking openness is the China rose branch that 7-8 becomes, the long 10-20cm of bar, lace 5-6 sheet leaf; Prune arrangement branch, leaf, spend scattered causing, the dust of washing branch gets final product infuse, for overcoming the branch come-up, ties up pressure with weight, submergence section 2-3cm; With the mixed stationary liquid fixation of 4ml concentration 40% formalin, 3g boric acid and 400ml water, time 8d; After fixation is stable, when non-pigment or impurity are separated out, take out and clean, immerse 20ml concentration 10% sulfurous acid, 10g boric acid and 1000ml water are preserved in the liquid, and paraffin envelope bottleneck is labelled; Preservation liquid to be replaced gets final product long preservation.
Embodiment 4
The infuse method of bur marigold sample:
Plucking openness is the Aztec marigold branch that 8-9 becomes, the long 10-20cm of bar; Flower band 9-10 sheet leaf; Prune arrangement, leaf, spend scattered causing, the dust of washing branch gets final product infuse, for overcoming the branch come-up, ties up pressure with weight, submergence section 2-3cm; With the mixed stationary liquid fixation of 40ml concentration 6% sulfurous acid and water 900ml, time 5d; After fixation is stable, when non-pigment or impurity are separated out, take out and clean, immerse 25ml formalin, in the preservation liquid of 25ml glycerine and 1000ml water, paraffin envelope bottleneck is labelled; But preservation liquid long preservation to be replaced.
Embodiment 5
Open the infuse method of the Ageratum conyzoides sample of purple flower:
Plucking openness is the Ageratum conyzoides branch that 8-9 becomes, the long 5-10cm of bar, 3-4 lace 9-10 sheet leaf; Prune arrangement branch, leaf, spend scattered causing, washing branch dust gets final product infuse, for overcoming the branch come-up, ties up pressure with weight, submergence section 2cm; Soak 1～4d with 20% magnesium chloride solution, soak successively with 30%, 50%, 75% magnesium chloride solution respectively then, soak 5～7d at every turn, PH is adjusted to 8, soak with 85% magnesium chloride solution respectively again, soak 7～10d at every turn, in oversaturated magnesium chloride fixer, keep primary colors; After fixation is stable, when non-pigment or impurity are separated out, take out and clean, the magnesium chloride solution of immersion 20% is preserved in the liquid, and paraffin envelope bottleneck is labelled; Preservation liquid to be replaced gets final product long preservation.
Claims (8)
1, the preparation method of eremophytes dipping specimen and guarantor's look, it is characterized in that: the making of dipping specimen comprises: selection collection → pruning cleaning → fixer infuse → preservation liquid infuse → go into bottle to seal a labelled step; It protects color fixing liquid:
Pink colour system flower fixer infuse: adopt in the mixed liquor of 1.8-4ml concentration 40% formalin, 2-4g boric acid, concentration 20% alcohol 8-12ml and 800-1200ml water, soak 22-26h; When non-pigment or impurity are separated out, move in the preservation liquid of 20-40ml concentration 6% sulfurous acid, 18-22g boric acid and 900-1100ml water, treat long preservation;
Green leaf, little design of scattered small flowers and plants series fixer infuse: adopt concentration 5% copper-bath, sample is soaked, this look section when presenting green → brown → yellow → green, the set time is 3-6d, shift out flush away copper sulphate, with concentration 2%～3% sulfurous acid bleaching 1-2d; When non-pigment or impurity are separated out, move in the preservation liquid of sulfurous acid, 38-42ml formaldehyde, 38-42ml glycerine, 900-1100ml water of 8-12ml concentration 6%, treat long preservation;
Red flower is the fixer infuse: adopt in the mixed liquor by 3-5ml concentration 40% formalin, 2-4g boric acid and 380-420ml water, soak 7-10d; When non-pigment or impurity are separated out, move in the preservation liquid of 18-22ml concentration 10% sulfurous acid, 8-12g boric acid and 900-1100ml water, treat long preservation;
Yellow is the fixer infuse: adopt in the formulated fixer of 38-42ml concentration 6% sulfurous acid and water 800-1000ml, soak 4-7d; When non-pigment or impurity are separated out, move in the preservation liquid of 23-27ml formalin, 23-27ml glycerine and 900-1100ml water, treat long preservation;
Peony, purple flower are the fixer infuse: adopt the magnesium chloride solution of concentration 20% to soak 2-5d, soak successively with the magnesium chloride solution of concentration 30%, 50%, 75% respectively then, soak 4-8d at every turn, control PH is 7.5-9; Soak with the magnesium chloride solution of concentration 85% respectively again, soak 6-10d at every turn after, in oversaturated magnesium chloride fixer, keep primary colors; When non-pigment or impurity are separated out, move in the preservation liquid of magnesium chloride solution of concentration 20%, treat long preservation.
2, the preparation method of dipping specimen according to claim 1 and guarantor's look is characterized in that: fixer with preservation liquid infusion step is:
The pink flower is the fixer infuse: adopt in the mixed liquor of 2-3ml concentration 40% formalin, 2-3g boric acid, concentration 20% alcohol 9-11ml and 900-1100ml water, soak 23-25h; When non-pigment or impurity are separated out, move in the preservation liquid of 25-35ml concentration 6% sulfurous acid, 19-21g boric acid and 950-1050ml water, treat long preservation;
Green leaf, little design of scattered small flowers and plants series fixer infuse: adopt concentration 5% copper-bath, sample is soaked, this look section when presenting green → brown → yellow → green, the set time is 3-5d, shift out flush away copper sulphate, with concentration 2%-3% sulfurous acid bleaching 1-1.5d; When non-pigment or impurity are separated out, move in the preservation liquid of sulfurous acid, 39-41ml formaldehyde, 39-41ml glycerine, 950-1050ml water of 9-11ml concentration 6%, treat long preservation;
Red flower is the fixer infuse: adopt in the mixed liquor by 3-5ml concentration 40% formalin, 2-4g boric acid and 380-420ml water, soak 7-9d; When non-pigment or impurity are separated out, move in the preservation liquid of 19-21ml concentration 10% sulfurous acid, 9-11g boric acid and 950-1050ml water, treat long preservation;
Yellow is the fixer infuse: adopt in the formulated fixer of 39-41ml concentration 6% sulfurous acid and water 850-950ml, soak 4-6d; When non-pigment or impurity are separated out, move in the preservation liquid of 24-26ml formalin, 24-26ml glycerine and 950-1050ml water, treat long preservation;
Peony, purple flower are the fixer infuse: adopt the magnesium chloride solution of concentration 20% to soak 2-4d, soak successively with the magnesium chloride solution of concentration 30%, 50%, 75% respectively then, soak 5-7d at every turn, control PH is 7.8-8.5; Soak with the magnesium chloride solution of concentration 85% respectively again, soak 7-9d at every turn after, in oversaturated magnesium chloride fixer, keep primary colors; When non-pigment or impurity are separated out, move in the preservation liquid of magnesium chloride solution of concentration 20%, treat long preservation.
3, the preparation method of dipping specimen according to claim 1 and guarantor's look is characterized in that: fixer with preservation liquid infusion step is:
The pink flower is the fixer infuse: adopt in the mixed liquor of 2ml concentration 40% formalin, 3g boric acid, concentration 20% alcohol 10ml and 1000ml water, soak 24h; When non-pigment or impurity are separated out, move in the preservation liquid of 30ml concentration 6% sulfurous acid, 20g boric acid and 1000ml water, treat long preservation;
Green leaf, little design of scattered small flowers and plants series fixer infuse: adopt concentration 5% copper-bath, sample is soaked, until the look section that presents green → brown → yellow → green, the set time is 4d, shifts out flush away copper sulphate, with concentration 2%～3% sulfurous acid bleaching 1d; When non-pigment or impurity are separated out, move in the preservation liquid of sulfurous acid, 40ml formaldehyde, 40ml glycerine, 1000ml water of 10ml concentration 6%, treat long preservation;
Red flower is the fixer infuse: adopt in the mixed liquor by 4ml concentration 40% formalin, 3g boric acid and 400ml water, soak 8d; When non-pigment or impurity are separated out, move in the preservation liquid of 20ml concentration 10% sulfurous acid, 10g boric acid and 1000ml water, treat long preservation;
Yellow is the fixer infuse: adopt in the formulated fixer of 40ml concentration 6% sulfurous acid and water 900ml, soak 5d; When non-pigment or impurity are separated out, move in the preservation liquid of 25ml formalin, 25ml glycerine and 1000ml water, treat long preservation;
Peony, purple flower are the fixer infuse: adopt the magnesium chloride solution of concentration 20% to soak 3d, soak successively with the magnesium chloride solution of concentration 30%, 50%, 75% respectively then, soak 6d at every turn, controlling PH is 8; Soak with the magnesium chloride solution of concentration 85% respectively again, soak 8d at every turn after, in oversaturated magnesium chloride fixer, keep primary colors; When non-pigment or impurity are separated out, move in the preservation liquid of magnesium chloride solution of concentration 20%, treat long preservation.
4, the preparation method of dipping specimen according to claim 1 is characterized in that: choosing is adopted sample and is got the feature typical case, the fresh anosis pityriasis simplex of branch, flower or fruit, and branch length is controlled at 10-20cm, and the openness of flower is preferably 7-8, and fruit is sharp very, full.
5, the preparation method of dipping specimen according to claim 1 is characterized in that: the branch of pruning, flower, fruit can't harm, and cleaning is removed dust, and plant submergence section is 1.8-2.5cm, uses the glass bar shaping, and loam cake seals, and lucifuge is deposited.
6, the preparation method of dipping specimen according to claim 1 is characterized in that: the sample clear water rinsing that infuse is good, and fixedly moulding, be immersed in and preserve in the liquid, paraffin sealing pastes tag along sort.
7, according to the preparation method of claim 1,2,3 described dipping specimens and guarantor's look, it is characterized in that: the fixer of dipping specimen recovers dip time 3-7 days of plant primary colors, meat, berry plant 60-90 days; Preserve and to use for a long time after liquid was changed in 1 year.
8, according to the preparation method of claim 1,2,3 described dipping specimens and guarantor's look, it is characterized in that: the container that sample infuse and fixation are deposited is glassware or pottery.
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| CNA2008100728371A CN101401567A (en) | 2008-03-19 | 2008-03-19 | Eremophyte steeping specimen and method for making protective color |
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